6 results on '"STYLIANOPOULOS, ANASTASIA"'
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2. Molecular characterization of Campylobacter spp. recovered from beef, chicken, lamb and pork products at retail in Australia.
- Author
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Wallace, Rhiannon L., Bulach, Dieter M., Jennison, Amy V., Valcanis, Mary, McLure, Angus, Smith, James J., Graham, Trudy, Saputra, Themy, Firestone, Simon, Symes, Sally, Waters, Natasha, Stylianopoulos, Anastasia, Kirk, Martyn D., and Glass, Kathryn
- Subjects
CAMPYLOBACTER ,PORK products ,BEEF products ,DRUG resistance in microorganisms ,BETA lactam antibiotics ,MACROLIDE antibiotics ,GENETIC mutation ,BEEF - Abstract
Australian rates of campylobacteriosis are among the highest in developed countries, yet only limited work has been done to characterize Campylobacter spp. in Australian retail products. We performed whole genome sequencing (WGS) on 331 C. coli and 285 C. jejuni from retail chicken meat, as well as beef, chicken, lamb and pork offal (organs). Campylobacter isolates were highly diverse, with 113 sequence types (STs) including 38 novel STs, identified from 616 isolates. Genomic analysis suggests very low levels (2.3โ15.3%) of resistance to aminoglycoside, beta-lactam, fluoroquinolone, macrolide and tetracycline antibiotics. A majority (>90%) of isolates (52/56) possessing the fluoroquinolone resistance-associated T86I mutation in the gyrA gene belonged to ST860, ST2083 or ST7323. The 44 pork offal isolates were highly diverse, representing 33 STs (11 novel STs) and harboured genes associated with resistance to aminoglycosides, lincosamides and macrolides not generally found in isolates from other sources. Prevalence of multidrug resistant genotypes was very low (<5%), but ten-fold higher in C. coli than C. jejuni. This study highlights that Campylobacter spp. from retail products in Australia are highly genotypically diverse and important differences in antimicrobial resistance exist between Campylobacter species and animal sources. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
3. Prevalence of Campylobacter coli and Campylobacter jejuni in Retail Chicken, Beef, Lamb, and Pork Products in Three Australian States.
- Author
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WALKER, LIZ J., WALLACE, RHIANNON L., SMITH, JAMES J., GRAHAM, TRUDY, SAPUTRA, THEMY, SYMES, SALLY, STYLIANOPOULOS, ANASTASIA, POLKINGHORNE, BENJAMIN G., KIRK, MARTYN D., and GLASS, KATHRYN
- Abstract
The aim of this study was to investigate the prevalence and distribution of Campylobacter species in a variety of fresh and frozen meat and offal products collected from retail outlets in New South Wales (NSW), Queensland (Qld), and Victoria (Vic). A total of 1,490 chicken, beef, lamb, and pork samples were collected from Australian supermarkets and butcher shops over a 2-year sampling period (October 2016 to October 2018). Campylobacter spp. were detected in 90% of chicken meat and 73% of chicken offal products (giblet and liver), with significantly lower prevalence in lamb (38%), pork (31%), and beef (14%) offal (kidney and liver). Although retail chicken meat was frequently contaminated with Campylobacter, the level of contamination was generally low. Where quantitative analysis was conducted, 98% of chicken meat samples, on average, had <10,000 CFU Campylobacter per carcass, with 10% <21 CFU per carcass. Campylobacter coli was the most frequently recovered species in chicken meat collected in NSW (53%) and Vic (56%) and in chicken offal collected in NSW (77%), Qld (59%), and Vic (58%). In beef, lamb, and pork offal, C. jejuni was generally the most common species (50 to 86%), with the exception of pork offal collected in NSW, where C. coli was more prevalent (69%). Campylobacter prevalence was significantly higher in fresh lamb (46%) and pork (31%) offal than in frozen offal (17 and 11%, respectively). For chicken, beef, and pork offal, the prevalence of Campylobacter spp. was significantly higher on delicatessen products compared with prepackaged products. This study demonstrated that meat and offal products are frequently contaminated with Campylobacter. However, the prevalence is markedly different in different meats, and the level of chicken meat portion contamination is generally low. By identifying the types of meat and offal products types that pose the greatest risk of Campylobacter infection to consumers, targeted control strategies can be developed. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
4. 832Detecting spatial clusters of human salmonellosis in Victoria.
- Author
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Morgan, Hannah, Cutcher, Zoe, Firestone, Simon, Stevenson, Mark, Stylianopoulos, Anastasia, Arnold, Anna-Lena, and Pfeiffer, Caitlin
- Subjects
SCAN statistic ,PUBLIC health surveillance ,SALMONELLA diseases ,STATISTICAL software ,ALGORITHMS ,DECISION making ,DATA integrity - Abstract
Focus of Presentation 'Cluster Tracker' is an automated tool for spatial cluster detection of notifiable disease data collected by the Department of Health (DH), Victoria. The tool combines R statistical software and a SaTScan cluster detection algorithm (prospective space-time permutation scan statistic) to detect notifiable disease case clusters in Victoria and is presently implemented for salmonellosis (categorised by type and/or MLVA). The objective of the tool is to conduct an initial screening of case data to improve the prioritisation of salmonellosis cases for epidemiological investigation. Findings The Cluster Tracker tool parameters have been validated using historical data from 2017-2018, comparing DH outbreak and cluster investigations identified by usual surveillance activities with clusters detected by the Cluster Tracker tool. Parameter selection considered cluster detection agreement and disagreement, disease-specific epidemiological characteristics, and operational requirements. The Cluster Tracker tool was able to provide closely-aligned agreement with existing DH outbreak and cluster investigations using the validated parameters. Implications This automated spatial cluster detection tool complements existing desktop surveillance of salmonellosis notifications to enhance public health decision making, and serves as an example of how spatial methods can improve real-time surveillance. Key messages Advanced spatial statistical tools have a role alongside traditional methods to make better use of limited epidemiological capacity and improve the timeliness and prioritisation of surveillance activities for notifiable diseases. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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5. Is penicillin and/or erythromycin resistance present in clinical isolates of group B streptococcus in our community?
- Author
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Stylianopoulos, Anastasia, Kelly, Nigel, and Garland, Suzanne
- Published
- 2002
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6. A foodborne outbreak of campylobacteriosis at a wedding - Melbourne, Australia, 2022.
- Author
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McAllister J, Gregory J, Adamopoulos J, Walsh M, Stylianopoulos A, Arnold AL, Stafford R, Andersson P, and Stewart T
- Subjects
- Humans, Case-Control Studies, Australia epidemiology, Disease Outbreaks, Diarrhea, Campylobacter Infections epidemiology, Gastroenteritis epidemiology
- Abstract
Campylobacter is the most common bacterial cause of foodborne gastroenteritis in Australia; however, outbreaks caused by the pathogen are relatively uncommon. In March 2022, the Victorian Department of Health was notified of a gastrointestinal illness in 20 guests following attendance at a wedding reception. Two of these individuals were notified with laboratory-confirmed campylobacteriosis, and an investigation was undertaken to identify the source of the infection and implement strategies to prevent further illness. A case-control study was conducted to determine the likely source of infection. Cases were defined as attendees of the wedding reception, with onset of diarrhoea and/or abdominal cramping 1-10 days after attending the function. Controls were randomly selected from the remaining list of non-ill guests. Cases and controls were interviewed using a standardised, menu-based questionnaire. Food preparation processes were documented, and food samples collected. A total of 29 wedding guests met the case definition. Cases reported onset of illness 2-5 days following the wedding and major symptoms included abdominal cramping (100%), diarrhoea (90%), headache (79%), and fever (62%). Two cases were hospitalised, one with ongoing secondary neurological sequelae. Illness was significantly associated with consumption of a duck breast brioche canapé containing duck liver parfait (odds ratio = 2.85; 95% confidence interval: 1.03-7.86). No leftover food samples were available for testing. The investigation found that the duck canapé was the likely vehicle of infection. Consistent with the literature on Campylobacter transmission, it is likely that inadequate cooking of the duck liver for the parfait was the contributing factor that led to illness. This highlights the risks posed by undercooked poultry dishes, and shows that education of food handlers remains a priority., (© Commonwealth of Australia CC BY-NC-ND.)
- Published
- 2023
- Full Text
- View/download PDF
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