1. Cell Adhesion to Unnatural Ligands Mediated by a Bifunctional Protein.
- Author
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Sánchez-Cortés, Juan, Bähr, Katinka, and Mrksich, Milan
- Subjects
- *
LIGANDS (Chemistry) , *CARBONIC anhydrase , *CELL adhesion , *PEPTIDES , *CELL communication - Abstract
This paper describes a molecular strategy to restore adhesion of cells to surfaces that otherwise do not present ligands that can mediate adhesion. The approach is based on a carbonic anhydrase fusion protein that binds benzenesulfonamides and that also includes the RGD peptide motif that can bind to cell-surface integrin adhesion receptors. In this way, the fusion protein can bind to a monolayer that presents the benzenesulfonamide ligand, thereby positioning the RGD peptide at the surface, where it can mediate the adhesion and spreading of cells. This strategy may provide a general method for promoting the adhesion of cells to non-natural surfaces or to defective biological matrices. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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