41 results on '"Ru, Zhengang"'
Search Results
2. Author Correction: Homology-mediated inter-chromosomal interactions in hexaploid wheat lead to specific subgenome territories following polyploidization and introgression
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Jia, Jizeng, Xie, Yilin, Cheng, Jingfei, Kong, Chuizheng, Wang, Meiyue, Gao, Lifeng, Zhao, Fei, Guo, Jingyu, Wang, Kai, Li, Guangwei, Cui, Dangqun, Hu, Tiezhu, Zhao, Guangyao, Wang, Daowen, Ru, Zhengang, and Zhang, Yijing more...
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- 2024
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3. Identification and fine mapping of a QTL-rich region for yield- and quality-related traits on chromosome 4BS in common wheat (Triticum aestivum L.)
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Li, Jinghui, Zhao, Huanhuan, Zhang, Minghu, Bi, Chan, Yang, Xiaoyuan, Shi, Xintian, Xie, Chaojie, Li, Baoyun, Ma, Guangbin, Ru, Zhengang, Hu, Tiezhu, and You, Mingshan
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- 2024
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4. Stem characteristics and yield of wheat is regulated to improve planting efficiency and reduce lodging risk by fertilizer rate and irrigation stage
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Feng, Suwei, Shi, Chenchen, Wang, Peiyu, Chang, Sujing, Hu, Tiezhu, and Ru, Zhengang
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- 2024
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5. Integrative multi-omics analysis reveals the underlying toxicological mechanisms of enrofloxacin on the growth of wheat seedling roots
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Wu, Xiaojun, Chen, Xiangdong, Zhang, Dazhong, Hu, Xigui, Ding, Weihua, Wang, Yuquan, Li, Gan, Dong, Na, Hu, Haiyan, Hu, Tiezhu, and Ru, Zhengang
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- 2024
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6. Cleaved amplified polymorphic sequence (CAP) marker development and haplotype geographic distribution of TaBOR1.2 associated with grain number in common wheat in China
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Wang, Yuquan, Hu, Xigui, Fu, Lina, Wu, Xiaojun, Niu, Zhipeng, Liu, Mingjiu, and Ru, Zhengang
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- 2023
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7. Genome resources for the elite bread wheat cultivar Aikang 58 and mining of elite homeologous haplotypes for accelerating wheat improvement
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Jia, Jizeng, Zhao, Guangyao, Li, Danping, Wang, Kai, Kong, Chuizheng, Deng, Pingchuan, Yan, Xueqing, Zhang, Xueyong, Lu, Zefu, Xu, Shujuan, Jiao, Yuannian, Chong, Kang, Liu, Xu, Cui, Dangqun, Li, Guangwei, Zhang, Yijing, Du, Chunguang, Wu, Liang, Li, Tianbao, Yan, Dong, Zhan, Kehui, Chen, Feng, Wang, Zhiyong, Zhang, Lichao, Kong, Xiuying, Ru, Zhengang, Wang, Daowen, and Gao, Lifeng more...
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- 2023
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8. Centromere repositioning and shifts in wheat evolution
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Zhao, Jing, Xie, Yilin, Kong, Chuizheng, Lu, Zefu, Jia, Haiyan, Ma, Zhengqiang, Zhang, Yijing, Cui, Dangqun, Ru, Zhengang, Wang, Yuquan, Appels, Rudi, Jia, Jizeng, and Zhang, Xueyong
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- 2023
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9. Identification and validation of QTLs for kernel number per spike and spike length in two founder genotypes of wheat
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Xu, Xin, Li, Xiaojun, Zhang, Dehua, Zhao, Jishun, Jiang, Xiaoling, Sun, Haili, and Ru, Zhengang
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- 2022
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10. Homology-mediated inter-chromosomal interactions in hexaploid wheat lead to specific subgenome territories following polyploidization and introgression
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Jia, Jizeng, Xie, Yilin, Cheng, Jingfei, Kong, Chuizheng, Wang, Meiyue, Gao, Lifeng, Zhao, Fei, Guo, Jingyu, Wang, Kai, Li, Guangwei, Cui, Dangqun, Hu, Tiezhu, Zhao, Guangyao, Wang, Daowen, Ru, Zhengang, and Zhang, Yijing more...
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- 2021
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11. Optimizing Wheat Planting Density by Adjusting Population Structure and Stabilizing Stem Strength to Achieve High and Stable Yields.
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Feng, Suwei, Shi, Chenchen, Wang, Peiyu, Chang, Sujing, Liu, Chaoyang, Shen, Chenwei, Li, Shilong, Hu, Tiezhu, and Ru, Zhengang
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PLANT spacing ,POPULATION density ,FIELD research ,PRODUCTION methods ,PLANT stems - Abstract
Increasing wheat (Triticum aestivum L.) planting density is the most effective production management method for increasing yields; however, excessive crop populations under high planting densities may experience elevated risk of stem lodging. We conducted this study to assess the relationship between reduced lodging and increased yield, investigate the effects of planting density on wheat population structure, stem strength, and material transport, and provide a basis for rationale planting densities. The experiments were carried out using a split-plot design with three replicates. The main plots contained two wheat varieties: Bainong 5819 (BN5819) and Bainong 4199 (BN4199), and the sub-plots contained four planting density treatments: 90 × 10
4 plants/ha (D1), 180 × 104 plants/ha (D2), 270 × 104 plants/ha (D3), and 360 × 104 plants/ha (D4). A two-year field trial was conducted in 2021–2023. The relationships between population structure characteristics, changes in stem strength, activation, and retransport of stem material after anthesis, and achievement of high and stable yields were investigated at the different planting densities. When the planting density of wheat increased from D1 to D4 treatment, the activity of fructan hydrolase was significantly increased. Compared with D1 treatment, the highest activity of fructan hydrolase was increased by 457.47 μg/h/g under D4 treatment. At the same time, the increase of density also increased the contribution rate of dry matter accumulation (CDMA) to grain after anthesis increased, with the highest increase in CDMA at 33.67%, which significantly reduced stem strength. Correlation analysis revealed a significant negative association between CDMA and stem strength. Specifically, CDMA levels were significantly lower with the D3 treatment than the D4 treatment, while stem strength remained higher after anthesis as an adaptive response to mitigate lodging risk. Stem storage compounds can promote grain filling and a weight increase in inferior grains. The number of spikes per unit area increased significantly with increasing planting density, but the number of grains per spike and 1000-grain weight decreased significantly. In two years, the number of spikes in D3 treatment increased by a maximum of 211.67 × 104 ha−1 and 99.17 × 104 ha−1 , respectively, compared to D1 and D2 treatments. The number of grains per spike was significantly higher than that of D4 treatment, the highest being 3.68 grains. Therefore, in the North China Plain with suitable water, fertilizer, and temperature, the sowing density of 270 × 104 plants/ha established population structure, significantly reduced CDMA, maintained post-anthesis stem strength, enhanced resilience of stems against post-anthesis lodging, and resulted in high yields by stabilizing the number of grains per spike and increasing the number of wheat spikes. [ABSTRACT FROM AUTHOR] more...- Published
- 2024
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12. Regulation of carbohydrate metabolism during anther development in a thermo‐sensitive genic male‐sterile wheat line.
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Gao, Huanting, Li, Dongxiao, Hu, Haiyan, Zhou, Feng, Yu, Yongang, Wei, Qichao, Liu, Qili, Liu, Mingjiu, Hu, Ping, Chen, Eryong, Song, Puwen, Su, Xiaojia, Guan, Yuanyuan, Qiao, Mei, Ru, Zhengang, and Li, Chengwei more...
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MALE sterility in plants ,CARBOHYDRATE metabolism ,METABOLIC regulation ,ANTHER ,SUCROSE ,WINTER wheat ,WHEAT - Abstract
Bainong sterility (BNS) is a thermo‐sensitive genic male sterile wheat line, characterised by anther fertility transformation in response to low temperature (LT) stress during meiosis, the failure of vacuole decomposition and the absence of starch accumulation in sterile bicellular pollen. Our study demonstrates that the late microspore (LM) stage marks the transition from the anther growth to anther maturation phase, characterised by the changes in anther structure, carbohydrate metabolism and the main transport pathway of sucrose (Suc). Fructan is a main storage polysaccharide in wheat anther, and its synthesis and remobilisation are crucial for anther development. Moreover, the process of pollen amylogenesis and the fate of the large vacuole in pollen are closely intertwined with fructan synthesis and remobilisation. LT disrupts the normal physiological metabolism of BNS anthers during meiosis, particularly affecting carbohydrate metabolism, thus determining the fate of male gametophytes and pollen abortion. Disruption of fructan synthesis and remobilisation regulation serves as a decisive event that results in anther abortion. Sterile pollen exhibits common traits of pollen starvation and impaired starch accumulation due to the inhibition of apoplastic transport starting from the LM stage, which is regulated by cell wall invertase TaIVR1 and Suc transporter TaSUT1. Summary statement: The manuscript provides new insight into the role of carbohydrate metabolism in regulating anther development and the molecular mechanisms underlying pollen abortion in a thermo‐sensitive genic male sterile winter wheat line under low temperature during meiosis. [ABSTRACT FROM AUTHOR] more...
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- 2024
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13. Low pH stress responsive transcriptome of seedling roots in wheat (Triticum aestivum L.)
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Hu, Haiyan, He, Jie, Zhao, Junjie, Ou, Xingqi, Li, Hongmin, and Ru, Zhengang
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- 2018
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14. Responses of root characteristics and nitrogen absorption and assimilation to different pH gradients of winter wheat at seedling stage.
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Shi, Chenchen, Wang, Peiyu, Wang, Guangtao, Hu, Tiezhu, Ru, Zhengang, and Feng, Suwei
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WINTER wheat ,WHEAT ,NITRATE reductase ,GLUTAMINE synthetase ,SOIL acidity ,SEEDLINGS - Abstract
Nitrogen (N) and rhizosphere pH are the two main factors restricting the growth of winter wheat (Triticum aestivum L.) in North China Plain. Soil nutrient availability is affected by soil acidity and alkalinity. In order to understand the effect of rhizosphere pH value on wheat nitrogen metabolism and the response of wheat growth to pH value at seedling stage, winter wheat varieties 'Aikang 58' (AK58) and 'Bainong 4199' (BN4199) were tested in hydroponics under three pH treatments (pH = 4.0, 6.5, and 9.0). The results showed that the accumulation of dry matter in root and above ground under pH 4.0 and pH 9.0 treatments was lower than that under pH 6.5 treatments, and the root/shoot ratio increased with the increase of pH value. Regardless of pH value, 'BN4199' had higher root dry weight, root length, root surface area, root activity and root tip than 'AK58'. Therefore, wheat that is tolerant to extreme pH is able to adapt to the acid-base environment by changing root characteristics. At pH 4.0, the net H
+ outflow rate of wheat roots was significantly lower than that of the control group, and the net NO3 − flux of wheat roots was also low. The net H+ outflow occurred at pH 6.5 and 9.0, and at the same time, the net NO3 − flux of roots also increased, and both increased with the increase of pH. The activity of nitrate reductase (NR) in stem of pH 9.0 treatment was significantly higher than that of other treatments, while the activity of glutamine synthetase (GS) in root and stem of pH 6.5 treatment was significantly higher than that of other treatments. Under pH 4.0 and pH 9.0 treatments, the activities of NR and GS in 'BN4199' were higher than those in 'AK58', The root respiration of 'BN4199' was significantly higher than that of 'AK58' under pH 4.0 and pH 9.0 treatment, and 'BN4199' had higher NO3 − net flux, key enzyme activity of root nitrogen metabolism and root respiration. Therefore, we believe that 'BN4199' has strong resistance ability to extreme pH stress, and high root/shoot ratio and strong root respiration can be used as important indicators for wheat variety screening adapted to the alkaline environment at the seedling stage. [ABSTRACT FROM AUTHOR] more...- Published
- 2023
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15. Genome-Wide Association Study on Seedling Phenotypic Traits of Wheat under Different Nitrogen Conditions.
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Hu, Chenchen, Li, Jinghui, Liu, Jiajia, Zhang, Dazhong, Jin, Liqiao, Yang, Nian, Bai, Bipo, Wang, Zenghao, Feng, Suwei, Ru, Zhengang, and Hu, Tiezhu
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GENOME-wide association studies ,OXIDOREDUCTASES ,ACYLTRANSFERASES ,NITROGEN fertilizers ,WHEAT ,PHENOTYPES ,SINGLE nucleotide polymorphisms - Abstract
Nitrogen fertilizer input is the main determinant of wheat yield, and heavy nitrogen fertilizer application causes serious environmental pollution. It is important to understand the genetic response mechanism of wheat to nitrogen and select wheat germplasm with high nitrogen efficiency. In this study, 204 wheat species were used to conduct genome-wide association analysis. Nine phenotypic characteristics were obtained at the seedling stage in hydroponic cultures under low-, normal, and high-nitrogen conditions. A total of 765 significant loci were detected, including 438, 261, and 408 single nucleotide polymorphisms (SNPs) associated with high-, normal, and low-nitrogen conditions, respectively. Among these, 14 SNPs were identified under three conditions, for example, AX-10887638 and AX-94875830, which control shoot length and root–shoot ratio on chromosomes 6A and 6D, respectively. Additionally, 39 SNPs were pleiotropic for multiple traits. Further functional analysis of the genes near the 39 SNPs shows that some candidate genes play key roles in encoding proteins/enzymes, such as transporters, hydrolases, peroxidases, glycosyltransferases, oxidoreductases, acyltransferases, disease-resistant proteins, ubiquitin ligases, and sucrose synthetases. Our results can potentially be used to develop low-nitrogen-tolerant species using marker-assisted selection and provide a theoretical basis for breeding efficient nitrogen-using wheat species. [ABSTRACT FROM AUTHOR] more...
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- 2023
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16. Improving Stem Lodging Resistance, Yield, and Water Efficiency of Wheat by Adjusting Supplemental Irrigation Frequency.
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Feng, Suwei, Shi, Chenchen, Wang, Peiyu, Ding, Weihua, Hu, Tiezhu, and Ru, Zhengang
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WATER efficiency ,IRRIGATION ,GRAIN yields ,DRY farming ,WINTER wheat ,WHEAT ,FIELD research ,STATISTICAL correlation - Abstract
Optimizing supplemental irrigation (SI) measures and enhancing stem lodging resistance can be the keys to achieving a high and stable yield and high efficiency in wheat. The experiment was established as a two-factor field trial in 2018–2020. We used four SI combinations at different stages: rain-fed (T0), SI at jointing (T1), SI at jointing + anthesis (T2), and SI at regreening + jointing + anthesis (T3) with 'Bainong4199' (BN4199) and 'Zhoumai18' (ZM18) as experimental materials. We researched the effects of different SI combinations on the stem characteristics, stem vigor, grain filling, and yield of winter wheat. The results suggest that the basal internode at the anthesis stage grew with the increase in SI amount, but the stem fracture resistance of T1 and T2 was higher than that of T0 and T3. As grain filling continued, the lodging index increased and stem vigor decreased. In comparison with T3, the average stem lodging index of T2 decreased by 21.92% for 'BN4199' and 36.63% for 'ZM18', but the WUE increased by 29.76% and 14.92%, respectively. The grain yield increased with the increase in irrigation times during the growth period; there was no significant difference between T2 and T3 in 2018–2019. In a biennial comparison, the grain yield of all treatments in 2019–2020 was significantly lower than those in 2018–2019, and the grain yield of 'ZM 18' was lower than that of 'BN 4199'. Correlation analysis displayed that there were significant positive correlations between post-anthesis stem vigor and the dry matter contribution rate of post-anthesis to grains and between the grain filling rate at 21–28 days after anthesis (DAA) and stem strength at 30 DAA. In summary, selecting a high-yield lodging-resistant wheat variety with SI at jointing and anthesis was beneficial for forming strong stems and maintaining higher stem vigor at the later growth stage for grain filling, which reduced lodging risk and ensured high yield and high WUE. [ABSTRACT FROM AUTHOR] more...
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- 2023
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17. Optical and physical mapping with local finishing enables megabase-scale resolution of agronomically important regions in the wheat genome
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Keeble-Gagnère, Gabriel, Rigault, Philippe, Tibbits, Josquin, Pasam, Raj, Hayden, Matthew, Forrest, Kerrie, Frenkel, Zeev, Korol, Abraham, Huang, B. Emma, Cavanagh, Colin, Taylor, Jen, Abrouk, Michael, Sharpe, Andrew, Konkin, David, Sourdille, Pierre, Darrier, Benoît, Choulet, Frédéric, Bernard, Aurélien, Rochfort, Simone, Dimech, Adam, Watson-Haigh, Nathan, Baumann, Ute, Eckermann, Paul, Fleury, Delphine, Juhasz, Angela, Boisvert, Sébastien, Nolin, Marc-Alexandre, Doležel, Jaroslav, Šimková, Hana, Toegelová, Helena, Šafář, Jan, Luo, Ming-Cheng, Câmara, Francisco, Pfeifer, Matthias, Isdale, Don, Nyström-Persson, Johan, IWGSC, Koo, Dal-Hoe, Tinning, Matthew, Cui, Dangqun, Ru, Zhengang, and Appels, Rudi more...
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- 2018
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18. Quantitative trait loci controlling amino acid contents in wheat ('Triticum aestivum' L.)
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Jiang, Xiaoling, Deng, Zhiying, Ru, Zhengang, Wu, Peng, and Tian, Jichun
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- 2013
19. Characterization of the complete mitochondrial genome of Elymus magellanicus (É.Desv.) Á.Löve (Poaceae, Pooideae).
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Chen, Xiangdong, Wu, Xiaojun, Zhang, Jinlong, Zhang, Man, You, Junchao, and Ru, Zhengang
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MITOCHONDRIAL DNA ,HORDEUM ,GRASSES ,MICROSATELLITE repeats ,BARLEY ,WHEAT - Abstract
Elymus magellanicus (É.Desv.) Á.Löve is a foliage accent plant that found in South America. In this study, the mitochondrial genome of E. magellanicus was sequenced, assembled, and annotated. The complete circular mitogenome of E. magellanicus is 583,450 bp in length and the overall G + C content of mitogenome is 42.27%. It harbors 39 protein-coding genes, 64 transfer RNA genes, eight ribosomal RNA genes, and 20 simple sequence repeats. Phylogenetic analysis indicates a relatively close relationship of E. magellanicus to Hordeum vulgare subsp. Spontaneum, Aegilops speltoides, Triticum aestivum, and T. aestivum cv. Chinese Yumai. [ABSTRACT FROM AUTHOR] more...
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- 2023
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20. Improvement of plant cryosection
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Zhang, Zhaopei, Niu, Liyuan, Chen, Xiling, Xu, Xu, and Ru, Zhengang
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- 2012
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21. Genetic Analysis and Molecular Mapping of a Novel Gene Conferring Resistance to Rice Stripe Virus
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Zhao, Feng, Cai, Zhijun, Hu, Tiezhu, Yao, Haigen, Wang, Li, Dong, Na, Wang, Bin, Ru, Zhengang, and Zhai, Wenxue
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- 2010
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22. Cloning a second form of adenine phosphoribosyl transferase gene ( TaAPT2) from wheat and analysis of its association with thermo-sensitive genic male sterility (TGMS)
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Xing, Quanhua, Ru, Zhengang, Li, Jun, Zhou, Chunjiang, Jin, Demin, Sun, Yan, and Wang, Bin
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- 2005
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23. Transcriptome analysis reveals important candidate genes involved in grain-size formation at the stage of grain enlargement in common wheat cultivar 'Bainong 4199'
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Gan Li, Guang Zhang, Wenhui Wei, Ru Zhengang, Xiao-Ling Jiang, Yang Zhang, Yuanyuan Guan, Zhaopu Wen, Yuquan Wang, and Zongli Chu
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0106 biological sciences ,0301 basic medicine ,Candidate gene ,Gene Expression ,01 natural sciences ,Biochemistry ,Starches ,Transcriptome ,Gene Expression Regulation, Plant ,Triticum ,Protein Metabolism ,Genetics ,Regulation of gene expression ,Multidisciplinary ,Organic Compounds ,Gene Ontologies ,food and beverages ,Eukaryota ,Genomics ,Plants ,Chemistry ,Wheat ,Physical Sciences ,Seeds ,Medicine ,Databases, Nucleic Acid ,Transcriptome Analysis ,Research Article ,Signal Transduction ,Science ,Carbohydrates ,Biology ,03 medical and health sciences ,Gene Regulation ,Grasses ,Common wheat ,KEGG ,Gene ,cDNA library ,Gene Expression Profiling ,Organic Chemistry ,Organisms ,Chemical Compounds ,Biology and Life Sciences ,Computational Biology ,Cell Biology ,Genome Analysis ,Gene expression profiling ,030104 developmental biology ,Metabolism ,010606 plant biology & botany - Abstract
Grain-size is one of the yield components, and the first 14 days after pollination (DAP) is a crucial stage for wheat grain-size formation. To understand the mechanism of grain-size formation at the whole gene expression level and to identify the candidate genes related to grain pattern formation, cDNA libraries from immature grains of 5 DAP and 14 DAP were constructed. According to transcriptome analysis, a total of 12,555 new genes and 9,358 differentially expressed genes (DEGs) were obtained. In DEGs, 2,876, 3,357 and 3,125 genes were located on A, B and D subgenome respectively. 9,937 (79.15%) new genes and 9,059 (96.80%) DEGs were successfully annotated. For DEGs, 4,453 were up-regulated and 4,905 were down-regulated at 14 DAP. The Gene Ontology (GO) database indicated that most of the grain-size-related genes were in the same cluster. The Kyoto Encyclopedia of Genes and Genomes (KEGG) database analysis showed that 130, 129 and 20 DEGs were respectively involved in starch and sucrose metabolism, plant hormone signal transduction and ubiquitin-mediated proteolysis. Expression levels of 8 randomly selected genes were confirmed by qRT-PCR, which was consistent with the transcriptome data. The present database will help us understand the molecular mechanisms underlying early grain development and provide the foundation for increasing grain-size and yield in wheat breeding programs. more...
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- 2019
24. A new, harmless, high-throughput endosperm-based DNA extraction method for wheat
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Wenhui Wei, Ru Zhengang, Zhihui Ma, and Yuquan Wang
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Population ,Triticum aestivum ,Trigo ,Endosperm ,lcsh:Agriculture ,Segregation population ,extração de DNA ,lcsh:Agriculture (General) ,education ,education.field_of_study ,General Veterinary ,biology ,endosperma ,Sequencia ,lcsh:S ,0402 animal and dairy science ,food and beverages ,04 agricultural and veterinary sciences ,biology.organism_classification ,lcsh:S1-972 ,040201 dairy & animal science ,DNA extraction ,Molecular biology ,SSR ,Agarose gel electrophoresis ,Wheat ,040103 agronomy & agriculture ,0401 agriculture, forestry, and fisheries ,Microsatellite ,Animal Science and Zoology ,Agronomy and Crop Science - Abstract
EnglishIn this study, a non-destructive, high-throughput, endosperm-based DNA extraction method was developed. To verify the nondestructive nature of this method, a germination test was performed on 288 seeds after sampling their endosperm, which gave a seedling emergence rate that was higher (97.6%) than that of the control group (92%). To confirm the feasibility of the new method, DNA was extracted from plants of a BC1F2 population by two different methods, namely, from endosperm using our rapid, high-throughput method (ER-DNAand from young leaves emerging from the same sampled seed using the CTAB method (LC-DNA). The ER-DNA was undetectable by agarose gel electrophoresis, but was found to be an adequate replacement for LC-DNA for the amplification and detection of simple sequence repeats (SSRs). Further analysis revealed that ER-DNA was generally suitable for the generation of specific 500–750-bp fragments, but not for the amplification of 1,000–2,000-bp fragments. Our rapid, high-throughput method therefore has no deleterious effects on wheat seeds and yields DNA for SSR genotyping that is a suitable alternative to traditionally obtained DNA. portuguesNeste estudo, foi desenvolvido um metodo de extracao de DNA nao destrutivo, de alto debito e endosperma. Para verificar a natureza nao destrutiva deste metodo, um teste de germinacao foi realizado em 288 sementes apos a amostragem do endosperma, o que deu uma taxa de emergencia de plântulas maior (97,6%) do que a do grupo controle (92%). Para confirmar a viabilidade do novo metodo, o DNA foi extraido de plantas de uma populacao de BC1F2 por dois metodos diferentes, a saber, do endosperma usando nosso metodo rapido de alto rendimento (ER-DNA) e de folhas jovens que emergem da mesma semente amostrada usando o metodo CTAB (LC-DNA). O ER-DNA foi indetectavel por eletroforese em gel de agarose, mas foi encontrado como uma substituicao adequada para LC-DNA para a amplificacao e deteccao de repeticoes simples de sequencia (SSRs). Uma analise posterior revelou que o ER-DNA era geralmente adequado para a geracao de fragmentos especificos de 500-750 pb, mas nao para a amplificacao de fragmentos de 1.000-2.000 pb. Nosso metodo rapido e de alto debito, portanto, nao tem efeitos deleterios sobre as sementes de trigo e produz DNA para a genotipagem de SSR que e uma alternativa adequada ao DNA obtido tradicionalmente more...
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- 2019
25. De novo assembly and characterization of the complete chloroplast genome of Elymus magellanicus (É.Desv.) Á.Löve, 1984 (Poaceae, Pooideae).
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Wu, Xiaojun, Chen, Xiangdong, Huang, Zhongwen, Ren, Cuicui, Hu, Tiezhu, and Ru, Zhengang
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CHLOROPLAST DNA ,GRASSES ,FOLIAGE plants ,TRANSFER RNA - Abstract
Elymus magellanicus (É.Desv.) Á.Löve is a foliage accent plant that originated in South America. In this study, the complete chloroplast genome of E. magellanicus is reported. It was found to have a total size of 133,249 bp. The chloroplast genome was found to consist of two inverted repeats (IRA and IRB) of 21,421 bp each, a small single-copy region of 12,709 bp, and a large single-copy region (77,698 bp). The annotation results show the GC content of the chloroplast genome to be 38.47%, including 40 tRNA genes, 82 protein-coding genes, and 8 rRNA genes. Phylogenetic analysis of 29 species revealed that E. magellanicus is closely related to E. arenarius. [ABSTRACT FROM AUTHOR] more...
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- 2022
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26. Dissection of Superior Alleles for Yield-Related Traits and Their Distribution in Important Cultivars of Wheat by Association Mapping.
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Li, Xiaojun, Xu, Xin, Liu, Weihua, Li, Xiuquan, Yang, Xinming, Ru, Zhengang, and Li, Lihui
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ALLELES in plants ,WHEAT genetics ,ALLELES ,WHEAT ,ECOLOGICAL regions ,CULTIVARS - Abstract
Uncovering the genetic basis of yield-related traits is important for molecular improvement of wheat cultivars. In this study, a genome-wide association study was conducted using the wheat 55K genotyping assay and a diverse panel of 384 wheat genotypes. The accessions used included 18 founder parents and 15 widely grown cultivars with annual maximum acreages of over 667,000 ha, and the remaining materials were elite cultivars and breeding lines from several major wheat ecological areas of China. Field trials were conducted in five major wheat ecological regions of China over three consecutive years. A total of 460 significant loci were detected for eight yield-related traits. Forty-five superior alleles distributed over 31 loci for which differences in phenotypic values grouped by single nucleotide polymorphism (SNP) reached significant levels (P < 0.05) in nine or more environments, were detected; some of these loci were previously reported. Eleven of the 31 superior allele loci on chromosomes 4A, 5A, 3B, 5B, 6B, 7B, 5D, and 7D had pleiotropic effects. For example, AX-95152512 on 5D was simultaneously related to increased grain weight per spike (GWS) and decreased plant height (PH); AX-109860828 on 5B simultaneously led to a high 1,000-kernel weight (TKW) and short PH; and AX-111600193 on 4A was simultaneously linked to a high TKW and GWS, and short PH. The favorable alleles in each accession ranged from 2 to 30 with an average of 16 at the thirty-one loci in the population, and six accessions (Zhengzhou683, Suzhou7829, Longchun7, Ningmai6, Yunmai35 and Zhen7630) contained more than 27 favorable alleles. A significant association between the number of favorable alleles and yield was observed (r = 0.799, p < 0.0001), suggesting that pyramiding multiple QTL with marker-assisted selection may effectively increase yield of wheat. Furthermore, distribution of superior alleles in founder parents and widely grown cultivars was also discussed here. This study is useful for marker-assisted selection for yield improvement and dissecting the genetic mechanism of important cultivars in wheat. [ABSTRACT FROM AUTHOR] more...
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- 2020
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27. Molecular cytogenetic identification of a novel wheat-Agropyron elongatum chromosome translocation line with powdery mildew resistance
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Xiaohui Gao, Xiao-Ling Jiang, Yajuan Xiao, Xiang-Dong Chen, Ru Zhengang, Jie Song, Li Xiaojun, and Cuicui Ren
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0106 biological sciences ,0301 basic medicine ,lcsh:Medicine ,Chromosomal translocation ,Plant Science ,01 natural sciences ,Translocation, Genetic ,Agropyron ,lcsh:Science ,In Situ Hybridization, Fluorescence ,Disease Resistance ,Genetics ,Expressed Sequence Tags ,Multidisciplinary ,biology ,medicine.diagnostic_test ,Fluorescent in Situ Hybridization ,Chromosome Biology ,Meiotic metaphase I ,Plant Fungal Pathogens ,food and beverages ,Agriculture ,Plants ,Chromosomal Aberrations ,Phenotype ,Wheat ,Cytogenetic Analysis ,Translocations ,Powdery mildew ,Research Article ,Genetic Markers ,Plant Pathogens ,Molecular Probe Techniques ,Plant disease resistance ,Research and Analysis Methods ,Chromosomes ,Chromosomes, Plant ,03 medical and health sciences ,Ascomycota ,medicine ,Grasses ,Molecular Biology Techniques ,Molecular Biology ,Plant Diseases ,lcsh:R ,Organisms ,Chromosome ,Biology and Life Sciences ,Cell Biology ,Plant Pathology ,biology.organism_classification ,Chromosome Pairs ,Powdery Mildew ,Probe Hybridization ,Agronomy ,Plant Breeding ,030104 developmental biology ,Genetic marker ,Seedlings ,lcsh:Q ,Thinopyrum ponticum ,Cytogenetic Techniques ,010606 plant biology & botany ,Fluorescence in situ hybridization ,Microsatellite Repeats - Abstract
Agropyron elongatum (Host.) Neviski (synonym, Thinopyrum ponticum Podp., 2n = 70) has been used extensively as a valuable source for wheat breeding. Numerous chromosome fragments containing valuable genes have been successfully translocated into wheat from A. elongatum. However, reports on the transfer of powdery mildew resistance from A. elongatum to wheat are rare. In this study, a novel wheat-A. elongatum translocation line, 11-20-1, developed and selected from the progenies of a sequential cross between wheat varieties (Lankaoaizaoba, Keyu 818 and BainongAK 58) and A. elongatum, was evaluated for disease resistance and characterized using molecular cytogenetic methods. Cytological observations indicated that 11-20-1 had 42 chromosomes and formed 21 bivalents at meiotic metaphase I. Genomic in situ hybridization analysis using whole genomic DNA from A. elongatum as a probe showed that the short arms of a pair of wheat chromosomes were replaced by a pair of A. elongatum chromosome arms. Fluorescence in situ hybridization, using wheat D chromosome specific sequence pAs1 as a probe, suggested that the replaced chromosome arms of 11-20-1 were 5DS. This was further confirmed by wheat SSR markers specific for 5DS. EST-SSR and EST-STS multiple loci markers confirmed that the introduced A. elongatum chromosome arms belonged to homoeologous group 5. Therefore, it was deduced that 11-20-1 was a wheat-A. elongatum T5DL∙5AgS translocation line. Both resistance observation and molecular marker analyses using two specific markers (BE443538 and CD452608) of A. elongatum in a F2 population from a cross between line 11-20-1 and a susceptible cultivar Yannong 19 verified that the A. elongatum chromosomes were responsible for the powdery mildew resistance. This work suggests that 11-20-1 likely contains a novel resistance gene against powdery mildew. We expect this line to be useful for the genetic improvement of wheat. more...
- Published
- 2017
28. A novel wheat lodging resistance evaluation method and device based on the thrust force of the stalks.
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Feng, Suwei, Kong, Dechuan, Ding, Weihua, Ru, Zhengang, Li, Gan, and Niu, Liyuan
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WHEAT ,EVALUATION methodology ,THRUST ,WIND speed ,WIND tunnels - Abstract
Wind speed is the most essential factor causing wheat lodging. Accurate understanding of the wind speed characteristics at near-surface layer of wheat fields and its effect on lodging is the basis of objective evaluation of wheat lodging resistance. In this paper, the characteristics of wind speed at the near-surface layer of wheat fields and their impact on lodging were studied. A new device was proposed for directly measuring the critical thrust force of wheat population lodging resistance in the field based on the black box method. A novel wheat stem lodging resistance evaluation method/model was established based on the critical thrust force of wheat population stem lodging and the wind speed characteristics of field near-surface layer. The method used the lodging critical wind speed as the index of wheat lodging resistance, which was verified by wind tunnel and field experiment. The results showed that there was a significant positive correlation between the critical wind speed of wheat lodging resistance and its critical thrust force. The values of wheat canopy apparent roughness length, wind attack angle, ventilation coefficient and other wind field characteristics had important effects on the calculation of wheat lodging resistance critical wind speed. The method can eliminate bias when calculating wheat lodging resistance by considering only one or a few indicators and the results of field lodging evaluation were consistent with those of field lodging survey. The method is simple and can be used to assess the lodging resistance of wheat, select extension regions for wheat varieties, and evaluate lodging factors in the field. [ABSTRACT FROM AUTHOR] more...
- Published
- 2019
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- View/download PDF
29. Transcriptome analysis reveals important candidate genes involved in grain-size formation at the stage of grain enlargement in common wheat cultivar “Bainong 4199”.
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Guan, Yuanyuan, Li, Gan, Chu, Zongli, Ru, Zhengang, Jiang, Xiaoling, Wen, Zhaopu, Zhang, Guang, Wang, Yuquan, Zhang, Yang, and Wei, Wenhui
- Subjects
GRAIN ,WHEAT breeding ,WHEAT ,GENES ,STARCH metabolism ,GENE expression - Abstract
Grain-size is one of the yield components, and the first 14 days after pollination (DAP) is a crucial stage for wheat grain-size formation. To understand the mechanism of grain-size formation at the whole gene expression level and to identify the candidate genes related to grain pattern formation, cDNA libraries from immature grains of 5 DAP and 14 DAP were constructed. According to transcriptome analysis, a total of 12,555 new genes and 9,358 differentially expressed genes (DEGs) were obtained. In DEGs, 2,876, 3,357 and 3,125 genes were located on A, B and D subgenome respectively. 9,937 (79.15%) new genes and 9,059 (96.80%) DEGs were successfully annotated. For DEGs, 4,453 were up-regulated and 4,905 were down-regulated at 14 DAP. The Gene Ontology (GO) database indicated that most of the grain-size-related genes were in the same cluster. The Kyoto Encyclopedia of Genes and Genomes (KEGG) database analysis showed that 130, 129 and 20 DEGs were respectively involved in starch and sucrose metabolism, plant hormone signal transduction and ubiquitin-mediated proteolysis. Expression levels of 8 randomly selected genes were confirmed by qRT-PCR, which was consistent with the transcriptome data. The present database will help us understand the molecular mechanisms underlying early grain development and provide the foundation for increasing grain-size and yield in wheat breeding programs. [ABSTRACT FROM AUTHOR] more...
- Published
- 2019
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30. Molecular cytogenetic identification of a novel wheat-Agropyron elongatum chromosome translocation line with powdery mildew resistance.
- Author
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Li, Xiaojun, Jiang, Xiaoling, Chen, Xiangdong, Song, Jie, Ren, Cuicui, Xiao, Yajuan, Gao, Xiaohui, and Ru, Zhengang
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WHEAT powdery mildew disease ,PLANT molecular genetics ,CYTOGENETICS ,PLANT chromosomes ,WHEATGRASSES ,PLANT translocation - Abstract
Agropyron elongatum (Host.) Neviski (synonym, Thinopyrum ponticum Podp., 2n = 70) has been used extensively as a valuable source for wheat breeding. Numerous chromosome fragments containing valuable genes have been successfully translocated into wheat from A. elongatum. However, reports on the transfer of powdery mildew resistance from A. elongatum to wheat are rare. In this study, a novel wheat-A. elongatum translocation line, 11-20-1, developed and selected from the progenies of a sequential cross between wheat varieties (Lankaoaizaoba, Keyu 818 and BainongAK 58) and A. elongatum, was evaluated for disease resistance and characterized using molecular cytogenetic methods. Cytological observations indicated that 11-20-1 had 42 chromosomes and formed 21 bivalents at meiotic metaphase I. Genomic in situ hybridization analysis using whole genomic DNA from A. elongatum as a probe showed that the short arms of a pair of wheat chromosomes were replaced by a pair of A. elongatum chromosome arms. Fluorescence in situ hybridization, using wheat D chromosome specific sequence pAs1 as a probe, suggested that the replaced chromosome arms of 11-20-1 were 5DS. This was further confirmed by wheat SSR markers specific for 5DS. EST-SSR and EST-STS multiple loci markers confirmed that the introduced A. elongatum chromosome arms belonged to homoeologous group 5. Therefore, it was deduced that 11-20-1 was a wheat-A. elongatum T5DL∙5AgS translocation line. Both resistance observation and molecular marker analyses using two specific markers (BE443538 and CD452608) of A. elongatum in a F
2 population from a cross between line 11-20-1 and a susceptible cultivar Yannong 19 verified that the A. elongatum chromosomes were responsible for the powdery mildew resistance. This work suggests that 11-20-1 likely contains a novel resistance gene against powdery mildew. We expect this line to be useful for the genetic improvement of wheat. [ABSTRACT FROM AUTHOR] more...- Published
- 2017
- Full Text
- View/download PDF
31. Sequencing and characterization of the complete mitochondrial genome of Thinopyrum obtusiflorum (DC.) Banfi, 2018 (Poaceae).
- Author
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Wu, Xiaojun, Hu, Xigui, Chen, Xiangdong, Zhang, Jinlong, Ren, Cuicui, Song, Lintong, Fang, Fang, Dong, Na, Hu, Tiezhu, and Ru, Zhengang
- Subjects
MICROSATELLITE repeats ,GRASSES ,WHEAT ,MITOCHONDRIA ,GENOMES - Abstract
In this study, the mitochondrial genome of Thinopyrum obtusiflorum was sequenced, assembled, and annotated. The complete circular mitogenome of Th. obtusiflorum is 390,725 bp in length and the overall A + T content of mitogenome is 55.61%. It harbors 33 protein-coding genes (PCGs), 21 transfer RNA genes (tRNAs), six ribosomal RNA genes (rRNAs), and 20 simple sequence repeats (SSRs). Phylogenetic analysis indicates that Th. obtusiflorum is a sister to the clade including Aegilops speltoides, Triticum aestivum, and Triticum aestivum cultivar Chinese Yumai in the Triticeae. [ABSTRACT FROM AUTHOR] more...
- Published
- 2022
- Full Text
- View/download PDF
32. Rapid determination of single-stalk and population lodging resistance strengths and an assessment of the stem lodging wind speeds for winter wheat
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Niu, Liyuan, Feng, Suwei, Ru, Zhengang, Li, Gang, Zhang, Zhaopei, and Wang, Zhiwei
- Subjects
- *
LODGING of crops , *PLANT stems , *WINTER wheat , *WIND speed , *PLANT populations , *WHEAT varieties - Abstract
Abstract: Lodging is a major limiting factor on wheat production worldwide but due to experimental restrictions, there is little accurate information on wheat single-stalk lodging resistance strength, particularly at the population level. The single-stalk and population lodging resistance strength of 10 winter wheat varieties (Triticum aestivum L.) were measured in the field between 2010 and 2012 using a crop lodging resistance electronic measuring device and, based on the population lodging resistance strength, wind speed and wind pressure theory, the population stem lodging wind speed for each wheat variety was estimated. The single-stalk lodging resistance strength was 2.50–10.79g-force stalk−1; the population lodging resistance strength was 20.35–65.06Nm−1 and the population stem lodging wind speed was 11.93–24.69ms−1 depending on variety and the growth stage. These results were consistent with general observations taken in the field and suggest that the method used in this study for population lodging resistance measurement and lodging wind speed evaluation may be suitable for the rapid identification of wheat variety lodging resistance in the field. [Copyright &y& Elsevier] more...
- Published
- 2012
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33. Optimizing the spatial distribution of roots by supplemental irrigation to improve grain yield and water use efficiency of wheat in the North China Plain.
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Feng, Suwei, Ding, Weihua, Shi, Chenchen, Zhu, Xiaoling, Hu, Tiezhu, and Ru, Zhengang
- Subjects
- *
WATER efficiency , *GRAIN yields , *WINTER wheat , *GRAIN , *IRRIGATION , *WHEAT , *SOIL management - Abstract
Soil moisture affects the yield and water use efficiency (WUE) of winter wheat (Triticum aestivum L.) by regulating the spatial distribution of roots. Therefore, appropriate supplemental irrigation (SI) schemes are essential to achieve high yield and high WUE of wheat by constructing a reasonable root structure. In this study, field experiments were conducted the North China Plain (NCP) from 2018 to 2020. Based on the soil moisture management at sowing on a consistent basis, four SI regimes were set up: no irrigation after seedling emergence (W0), SI at jointing (W1), SI at jointing and anthesis (W2), and SI at regreening, jointing, and anthesis (W3). The results showed that although the grain yield of wheat increased with the SI frequency, the WUE decreased with increasing SI amount. SI at jointing and anthesis significantly increased WUE without reducing or slightly reducing the grain yield. The grain yield and WUE in 2018–2019 were significantly higher than those of the corresponding treatments in 2019–2020, and the yield of Bainong 4199 was higher than that of Zhoumai 18. The grain yield and WUE in 2018–2019 were significantly higher than those of the corresponding treatments in 2019–2020. The root length density (RLD) in the 0–20-cm soil layer increased with increasing SI frequency, but the proportion of RLD in the 40–60-cm soil layer decreased. The average RLD in the 0–60-cm soil layer and grain yield of Bainong 4199 were significantly greater than that of Zhoumai 18. Correlation analysis showed that the grain yield and WUE was positively correlated with the RLD in the 0–20-cm soil layer, and reached a very significant level with RLD in the 0–20-cm soil layer between rows. W2 and W3 in terms of root vigor and the grain filling rate 30 days after anthesis were significantly higher than W1 and W0 and also increased the contribution of post-anthesis dry matter to grain, resulting in an increase in grain weight. These results demonstrated that SI at jointing and anthesis could optimize the spatial distribution of roots, maintain higher root activity and filling rate at the late stage, increase accumulation and translocation of post-anthesis dry matter, and finally improve the yield and WUE. Therefore, we propose that Bainong 4199 was the preferred cultivars for this experiment,under sufficient soil moisture conditions before sowing, SI at jointing and anthesis is the optimal irrigation scheme for wheat on the NCP. • Increasing RLD in the 0–20-cm soil layer is beneficial to improve yield and WUE. • Reasonable root spatial distribution is very important to improve yield and WUE. • Selecting wheat varieties with higher yield is more conducive to the improvement of water use efficiency. • High WUE was achieved by SI at joining and anthesis without reducing or with slight reduction grain yield. [ABSTRACT FROM AUTHOR] more...
- Published
- 2023
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34. Helitron and CACTA DNA transposons actively reshape the common wheat - AK58 genome.
- Author
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Wang, Zhiyong, Zhao, Guangyao, Yang, Qinghua, Gao, Lifeng, Liu, Chunyuan, Ru, Zhengang, Wang, Daowen, Jia, Jizeng, and Cui, Dangqun
- Subjects
- *
TRANSPOSONS , *PLANT genomes , *GENOMES , *DNA , *WHEAT , *SEED dormancy - Abstract
Transposable elements (TEs) play a pivotal role in reshaping the plant genome. Helitrons represent a new class of transposable elements recently discovered in animals and plants. Helitrons , DNA transposons that replicate via a rolling-circle replication mechanism, are a major driving force behind genome evolution. Since the recent divergence of the modern cultivars (e.g., AK58) and landraces (e.g., Chinese Spring), Helitrons appear to have contributed greatly to genome variability. We first identified 214,665 Helitrons in AK58 by HelitronScanner software and further detected 18,668 tandem duplicated Helitron regions (TDHRs) from all the Helitrons identified. There are 39% of TDHRs (7289) translocated since the divergence of the AK58 and Chinese Spring genomes. What interested us even more are the 462 TDHRs exclusive to the AK58 genome. We also found 235 TDHRs in the 21 centromeric regions and these TDHRs contributed to centromere plasticity. Another very interesting DNA transposon, CACTA, accounting for 15% of AK58 genome, was also the focus of this study because they often inserted into gene rich regions. We found that CACTAs have inserted into many agronomically important genes, such as seed dormancy gene TaMFT and vernalization gene TaVrn1 , indicating the important role of CACTAs in modern wheat adaptation. • Our search led to the identification of 18,668 tandem duplicated regions potentially mediated by Helitrons, (TDHRs), of which significant translocations (39%) occurred after the divergence between AK58 and Chinese Spring. • Of importance, we discovered 462 THDRs exclusive to AK58 and 235 TDHRs localized to centromeric regions potentially contributing to genome plasticity. • From these observations we conclude the Helitrons contributed immensely towards the domestication of wheat. • We also perform analysis of the CACTA family of transposable elements which constitutes ~15% of the genome and their insertion into genes bestowing agronomic important traits as an evidence into their important role in adaptation. [ABSTRACT FROM AUTHOR] more...
- Published
- 2022
- Full Text
- View/download PDF
35. Characterization of the complete chloroplast genome of Chasmanthium latifolium (Michx.) H.O.Yates, 1966 (Poaceae).
- Author
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Fang G, Jiang H, Zhang J, Wang R, Chen X, Hu T, Wu X, and Ru Z
- Abstract
Chasmanthium latifolium (Michx.) H.O.Yates is a popular ornamental plant native to southeastern North America. Genomic data and genetic studies related to Chasmanthium latifolium are limited. Therefore, the complete chloroplast genome of Chasmanthium latifolium was sequenced, assembled, and characterized in this study. The complete chloroplast genome was 138,934 bp in length and contained 105 unique genes (77 protein-coding genes, 24 tRNA genes, and 4 rRNA genes). Phylogenetic analyses showed that Chasmanthium latifolium and Chasmanthium laxum clustered into a separate clade with the closest affinity to the clade comprising Zeugites pittieri Hack and Lophatherum gracile Brongn. In conclusion, our study describes the complete chloroplast genome of Chasmanthium latifolium for the first time, contributing to a better understanding of its taxonomy and evolution., Competing Interests: No potential conflict of interest was reported by the author(s)., (© 2025 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.) more...
- Published
- 2025
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- View/download PDF
36. Characterization of the complete mitochondrial genome of Elymus magellanicus (É.Desv.) Á.Löve (Poaceae, Pooideae).
- Author
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Chen X, Wu X, Zhang J, Zhang M, You J, and Ru Z
- Abstract
Elymus magellanicus (É.Desv.) Á.Löve is a foliage accent plant that found in South America. In this study, the mitochondrial genome of E. magellanicus was sequenced, assembled, and annotated. The complete circular mitogenome of E. magellanicus is 583,450 bp in length and the overall G + C content of mitogenome is 42.27%. It harbors 39 protein-coding genes, 64 transfer RNA genes, eight ribosomal RNA genes, and 20 simple sequence repeats. Phylogenetic analysis indicates a relatively close relationship of E. magellanicus to Hordeum vulgare subsp. Spontaneum , Aegilops speltoides , Triticum aestivum , and T. aestivum cv. Chinese Yumai., Competing Interests: No potential conflict of interest was reported by the authors., (© 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.) more...
- Published
- 2023
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- View/download PDF
37. De novo assembly and characterization of the complete chloroplast genome of Elymus magellanicus (É.Desv.) Á.Löve, 1984 (Poaceae, Pooideae).
- Author
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Wu X, Chen X, Huang Z, Ren C, Hu T, and Ru Z
- Abstract
Elymus magellanicus (É.Desv.) Á.Löve is a foliage accent plant that originated in South America. In this study, the complete chloroplast genome of E. magellanicus is reported. It was found to have a total size of 133,249 bp. The chloroplast genome was found to consist of two inverted repeats (IRA and IRB) of 21,421 bp each, a small single-copy region of 12,709 bp, and a large single-copy region (77,698 bp). The annotation results show the GC content of the chloroplast genome to be 38.47%, including 40 tRNA genes, 82 protein-coding genes, and 8 rRNA genes. Phylogenetic analysis of 29 species revealed that E. magellanicus is closely related to E. arenarius ., Competing Interests: No potential conflict of interest was reported by the author(s)., (© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.) more...
- Published
- 2022
- Full Text
- View/download PDF
38. Molecular characterization of the genome-wide BOR transporter family and their responses to boron conditions in common wheat ( Triticum aestivum L.).
- Author
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Wang Y, Niu Z, Hu X, Wu X, Yang Z, Hao C, Zhou M, Yang S, Dong N, Liu M, and Ru Z
- Abstract
Boron (B) deficiency is an agricultural problem that causes significant yield losses in many countries. B transporters ( BOR s) are responsible for B uptake and distribution and play important roles in yield formation. A comprehensive analysis of the BOR family members in common wheat is still lacking. In the present study, to clarify the molecular characterization and response to B status, genome-wide TaBOR genes and expression patterns were investigated. Fourteen TaBOR genes were identified in common wheat by a homology search. The corresponding phylogenetic tree indicated that 14 TaBOR genes were separately classified into subfamilies of TaBOR1 , TaBOR3 , and TaBOR4 . All TaBOR genes had 12-14 extrons and 11-13 introns. Most TaBOR proteins contained 10 conserved motifs, and motifs 1, 2, 3, 4, and 6 constituted the conserved bicarbonate (HCO
3 - ) domain. Fourteen TaBOR genes were mapped on 13 chromosomes mainly distributed in the first, third, fifth, and seventh homologous groups. The promoters of TaBOR genes consisted of phytohormones, light responses, and stress-related cis-elements. GO analysis indicated that TaBOR genes were enriched in terms of transmembrane transport and ion homeostasis. TaBOR genes showed diverse expression profiles in different tissues. The members of the TaBOR1 subfamily showed high expression in grains, leaves, roots, stems, and spikes, but members of the TaBOR4 subfamily were highly expressed only in spikes and grains. RT-qPCR indicated that TaBOR1-5A , TaBOR1-5B , and TaBOR1-5D were induced by low B concentrations and had much higher expression in roots than in shoots. TaBOR3-3A , TaBOR3-3B , TaBOR3-3D , TaBOR4-1A , TaBOR4-1B , TaBOR4-1D , and TaBOR3-4B were induced by low and high B concentrations and had high expression in roots and shoots. TaBOR3-4D and TaBOR3-7B were upregulated by low and high B concentrations, respectively, but had expression only in roots. Our results provide basic information on the TaBOR family, which is beneficial for elucidating the functions of TaBOR genes to overcome the problem of B deficiency., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Wang, Niu, Hu, Wu, Yang, Hao, Zhou, Yang, Dong, Liu and Ru.) more...- Published
- 2022
- Full Text
- View/download PDF
39. Sequencing and characterization of the complete mitochondrial genome of Thinopyrum obtusiflorum (DC.) Banfi, 2018 (Poaceae).
- Author
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Wu X, Hu X, Chen X, Zhang J, Ren C, Song L, Fang F, Dong N, Hu T, and Ru Z
- Abstract
In this study, the mitochondrial genome of Thinopyrum obtusiflorum was sequenced, assembled, and annotated. The complete circular mitogenome of Th. obtusiflorum is 390,725 bp in length and the overall A + T content of mitogenome is 55.61%. It harbors 33 protein-coding genes (PCGs), 21 transfer RNA genes (tRNAs), six ribosomal RNA genes (rRNAs), and 20 simple sequence repeats (SSRs). Phylogenetic analysis indicates that Th. obtusiflorum is a sister to the clade including Aegilops speltoides , Triticum aestivum , and Triticum aestivum cultivar Chinese Yumai in the Triticeae., Competing Interests: No potential conflict of interest was reported by the author(s)., (© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.) more...
- Published
- 2022
- Full Text
- View/download PDF
40. A Ricin B-Like Lectin Protein Physically Interacts with TaPFT and Is Involved in Resistance to Fusarium Head Blight in Wheat.
- Author
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Song P, Zhang L, Wu L, Hu H, Liu Q, Li D, Hu P, Zhou F, Bu R, Wei Q, Yu Y, Guan Y, Chen E, Su X, Huang Z, Qiao M, Ru Z, and Li C
- Subjects
- Disease Resistance genetics, Plant Diseases, Quantitative Trait Loci, Triticum genetics, Fusarium, Ricin
- Abstract
Fusarium head blight (FHB), mainly caused by Fusarium graminearum , has become one of the most serious diseases that damage wheat. The TaPFT (pore-forming toxin-like) and TaHRC (histidine-rich calcium-binding protein) genes at the quantitative trait locus Fhb1 were identified to confer resistance to FHB in the wheat cultivar Sumai 3. In this study, a wheat ricin B-like lectin gene (designated TaRBL ) that interacted with TaPFT was isolated by a yeast two-hybrid screen of a wheat cDNA library. A yeast two-hybrid and bimolecular fluorescence complementation study further verified that TaRBL interacted with TaPFT but not with TaHRC. Gene expression studies showed that upon F. graminearum infection, TaRBL expression was upregulated in resistant cultivars but downregulated in susceptible cultivars. Furthermore, knockdown of TaRBL expression by barley stripe mosaic virus-induced gene silencing significantly reduced the resistance of wheat to FHB in both the resistant cultivar Sumai 3 and the susceptible cultivar Jimai 22. Thus, we conclude that TaRBL encodes a ricin B-like lectin protein that interacts with TaPFT and is involved in resistance to FHB in wheat. more...
- Published
- 2021
- Full Text
- View/download PDF
41. Comparison of A and B starch granules from three wheat varieties.
- Author
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Zeng J, Li G, Gao H, and Ru Z
- Subjects
- Amylose analysis, Calorimetry, Differential Scanning, Chemical Fractionation, Spectroscopy, Fourier Transform Infrared, Starch ultrastructure, Temperature, X-Ray Diffraction, Starch chemistry, Triticum chemistry
- Abstract
Three starches from the wheat varieties AK58, ZM18 and YZ4110 were separated into large (A) and small (B) granules, which were characterized structurally and evaluated for their functional properties. SEM results showed that the size of A-granules from ZM18 and YZ4110 were about the same, but the sizes of A-granules and B-granules from AK58 were larger than those of ZM18 and YZ4110. FTIR spectra showed that all the samples exhibited a similar pattern, with seven main modes with maximum absorbance peaks near 3,500, 3,000, 1,600, 1,400, 1,000, 800, 500 cm-1. The B-granules of ZM18 and YZ4110 had less amylose content, although the difference among the total amylose contents of the three unfractionated starches was not significant. X-ray diffraction (XRD) patterns showed predominantly A-type crystallinity for all the starches. The A-granules showed sharper XRD patterns than the other starches. DSC analysis showed that the A-granules had broader ranges of gelatinization temperatures than the B-granules from the same wheat variety. The gelatinization enthalpy (ΔH) of A-granules was higher than that of B-granules. AK58 exhibited the smallest enthalpy, while ZM18 showed the largest enthalpy. In pasting tests, the A-granule starch of AK58 had higher peak, final and setback viscosity, lower breakdown and pasting temperature, and the B-granule starch and unfractionated starch of AK58 had lower peak, breakdown, final and setback viscosity and higher pasting temperature than ZM18 and YZ4110. more...
- Published
- 2011
- Full Text
- View/download PDF
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