33 results on '"Royet, J.-P."'
Search Results
2. Subliminal fear priming potentiates negative facial reactions to food pictures in women with anorexia nervosa
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Soussignan, R., Jiang, T., Rigaud, D., Royet, J. P., and Schaal, B.
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- 2010
3. Role of verbal encoding in shortand long-term odor recognition
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Jehl, C., Royet, J. P., and Holley, A.
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- 1997
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4. Olfactory hypersensitivity in migraineurs: a H215O-PET study
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Demarquay, G, Royet, J P, Mick, G, and Ryvlin, P
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- 2008
5. Odor discrimination and recognition memory as a
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Jehl, C., Royet, J. P., and Holley, A.
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- 1995
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6. Rating of olfactory judgements in migraine patients
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Demarquay, G, Royet, J P, Giraud, P, Chazot, G, Valade, D, and Ryvlin, P
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- 2006
7. Very short term recognition memory for odors
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Jehl, C., Royet, J. -P., and Holley, A.
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- 1994
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8. Evaluation of the precision of systematic sampling: Nugget effect and covariogram modelling.
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THIOULOUSE, J., ROYET, J. P., PLOYE, H., and HOULLIER, F.
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- 1993
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9. Is Short-term Odour Recognition Predictable from Odour Profile?
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Royet, J.-P., Paugam-Moisy, H., Rouby, C., Zighed, D., Nicoloyannis, N., Amghar, S., and Sicard, G.
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- 1996
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10. Morphometric modifications associated with early sensory experience in the rat olfactory bulb: I. Volumetric study of the bulbar layers.
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Royet, J. P., Jourdan, F., and Ploye, H.
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- 1989
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11. Morphometric modifications associated with early sensory experience in the rat olfactory bulb: II. Stereological study of the population of olfactory glomeruli.
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Royet, J. P., Jourdan, F., Ploye, H., and Souchier, C.
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- 1989
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12. Morphometric study of the glomerular population in the mouse olfactory bulb: Numerical density and size distribution along the rostrocaudal axis.
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Royet, J. P., Souchier, C., Jourdan, F., and Ploye, H.
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- 1988
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13. Both of Us Disgusted in My Insula The Common Neural Basis of Seeing and Feeling Disgust
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Wicker, B, Keysers, C, Plailly, J, Royet, J P, Gallese, V, and Rizzolatti, G
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OLD-WORLD MONKEY ,FACIAL EXPRESSIONS ,PREMOTOR CORTEX ,TEMPORAL CORTEX ,RECOGNITION ,EMOTION ,PAIN ,HUMAN CINGULATE CORTEX ,NEURONS ,HUMAN AMYGDALA ,humanities - Abstract
What neural mechanism underlies the capacity to understand the emotions of others? Does this mechanism involve brain areas normally involved in experiencing the same emotion? We performed an fMRI study in which participants inhaled odorants producing a strong feeling of disgust. The same participants observed video clips showing the emotional facial expression of disgust. Observing such faces and feeling disgust activated the same sites in the anterior insula and to a lesser extent in the anterior cingulate cortex. Thus, as observing hand actions activates the observer's motor representation of that action, observing an emotion activates the neural representation of that emotion. This finding provides a unifying mechanism for understanding the behaviors of others.
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14. 5-hydroxytryptamine action in the rat olfactory bulb: In vitro electrophysiological patch-clamp recordings of juxtaglomerular and mitral cells
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Hardy, A., Palouzier-Paulignan, B., Duchamp, A., Royet, J.-P., and Duchamp-Viret, P.
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SEROTONIN , *NEUROTRANSMITTERS , *GABA , *CELLS - Abstract
Abstract: The olfactory bulb, first relay of olfactory pathways, is densely innervated by serotoninergic centrifugal fibers originating from the raphe nuclei. Although serotonin innervation was reported to be involved in olfactory learning in mammals, the action of this neurotransmitter on its putative cellular targets has been never described through unitary recordings. This lack of data initiated the present study where the effects of 5HT on juxtaglomerular and mitral cells are analyzed using whole-cell recordings on olfactory bulb slices. Serotonin depolarizes 34% of 525 JG cells. A multivariate statistical analysis of juxtaglomerular cells characteristics shows that the serotonin responsive cell group can be individualized regarding their tonic discharge-mode in response to a direct current injection, their lower expression of hyperpolarization-activated cation current and their low membrane capacities. The use of ion channel blockers and ramp voltage protocol indicate that serotoninergic depolarization of juxtaglomerular cells may be due to a nonselective cation current with a reversal potential of −44 mV. Pharmacological tests with serotonin receptor antagonists and agonists reveal that 5HT action on juxtaglomerular cells would be mainly mediated by 5HT2C receptors. In mitral cells, serotonin acts on 49.1% of the 242 tested cells, inducing two types of responses. A first subset of mitral cells (26.8%, n=65) were hyperpolarized by serotonin. This response would be indirect and mediated by action of GABA on GABAA receptors since it was antagonized by bicuculline. The involved GABAergic neurons are hypothesized to be juxtaglomerular and granular cells, on which serotonin would act mainly via 5HT2C and via 5HT2A receptors respectively. The second subset of mitral cells (22.3%, n=54) were directly depolarized by serotonin acting through 5HT2A receptors. Our data on serotonin action on juxtaglomerular cells and mitral cells reveal a part of functional mechanisms whereby serotonin can act on olfactory bulb network. This is expected to enrich the understanding of its determining role in olfactory learning. [Copyright &y& Elsevier]
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- 2005
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15. Brainstem changes in 5-HT1A receptor availability during migraine attack.
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Demarquay G, Lothe A, Royet JP, Costes N, Mick G, Mauguière F, and Ryvlin P
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- Adult, Humans, Image Processing, Computer-Assisted, Migraine Disorders drug therapy, Piperazines, Positron-Emission Tomography, Radiopharmaceuticals, Serotonin 5-HT1 Receptor Antagonists therapeutic use, Brain Stem diagnostic imaging, Brain Stem metabolism, Migraine Disorders diagnostic imaging, Migraine Disorders metabolism, Receptor, Serotonin, 5-HT1A biosynthesis
- Abstract
Background: Among serotonin receptors, 5-HT(1A) receptors are implicated in the regulation of central serotoninergic tone and could be involved in the abnormal brain 5-HT turnover suspected in migraineurs. The aim of this study was to investigate 5-HT(1A) receptors' availability during migraine attacks., Methods: Ten patients suffering from odor-triggered migraine attacks and 10 control subjects were investigated using positron emission tomography (PET) and [(18)F]MPPF PET tracer, a selective 5-HT(1A) antagonist. All subjects underwent calibrated olfactory stimulations prior to the PET study., Results: Four patients developed a migraine attack during the PET study. In these patients, statistical parametrical mapping and region of interest analyses showed an increased [(18)F]MPPF binding potential (BP(ND)) in the pontine raphe when compared to headache-free migraineurs and control subjects. This ictal change was confirmed at the individual level in each of the four affected patients. In comparison with the headache-free migraineurs, patients with a migraine attack also showed significantly increased [(18)F]MPPF BP(ND) in the left orbitofrontal cortex, precentral gyrus and temporal pole. No significant change in [(18)F]MPPF BP(ND) was observed between headache-free migraineurs and controls., Conclusions: Our results emphasize the role of 5HT(1A) receptors in the pontine raphe nuclei during the early stage of migraine attacks.
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- 2011
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16. Cerebral imaging and olfactory disorders: a review.
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Plailly J, Demarquay G, and Royet JP
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- Humans, Reproducibility of Results, Cerebrum, Diagnostic Imaging methods, Olfaction Disorders diagnosis
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Cerebral imaging and olfactory disorders: a review. Olfactory disorders are often misjudged and rarely given due clinical consideration. Nevertheless, they occur in a wide range of neurological disorders, and their evaluation can help in diagnosis. Whereas psychophysical tests have been used to evaluate olfactory dysfunction in numerous diseases, functional brain imaging using olfactory stimuli is an emergent technique and few studies have been published to date. After a reminder of cerebral imaging and analysis techniques and a rapid description of our actual knowledge of olfactory processes in healthy subjects, the current review focuses on cerebral imaging studies performed on patients with neurological disorders and presenting olfactory dysfunction. Neurological disorders such as Alzheimer's disease, Parkinson's disease, epilepsy, migraine, multiple chemical sensitivity and schizophrenia are examined.
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- 2009
17. A stimulation method using odors suitable for PET and fMRI studies with recording of physiological and behavioral signals.
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Vigouroux M, Bertrand B, Farget V, Plailly J, and Royet JP
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- Adult, Behavioral Sciences instrumentation, Behavioral Sciences methods, Brain anatomy & histology, Brain physiology, Brain Mapping instrumentation, Brain Mapping methods, Drug Delivery Systems instrumentation, Female, Galvanic Skin Response physiology, Humans, Magnetic Resonance Imaging, Magnetics, Optics and Photonics, Physical Stimulation instrumentation, Physical Stimulation methods, Plethysmography, Positron-Emission Tomography, Respiratory Physiological Phenomena, Signal Processing, Computer-Assisted instrumentation, Drug Delivery Systems methods, Odorants, Smell physiology
- Abstract
A design for a semi-automatic olfactometric system is described for PET and fMRI experiments. The olfactometer presents several advantages because it enables the use of an 'infinite' number of odorants and the synchronization of stimuli with breathing. These advantages mean that the subject is recorded while breathing normally during olfactory judgment tasks. In addition, the design includes a system for recording the behavioral (rating scale) and physiological (breathing, electrodermal reaction (ED), plethysmography (PL)) signals given by the subject. Both systems present the advantage of being compatible with fMRI magnetic fields since no ferrous material is used in the Faraday cage and signals are transmitted via an optical transmission interface to an acquisition system.
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- 2005
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18. Odorants elicit evoked potentials in the human amygdala.
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Hudry J, Ryvlin P, Royet JP, and Mauguière F
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- Adolescent, Adult, Electrodes, Implanted, Electroencephalography, Epilepsies, Partial diagnosis, Epilepsies, Partial surgery, Female, Humans, Male, Middle Aged, Sensory Thresholds physiology, Stimulation, Chemical, Amygdala physiology, Evoked Potentials physiology, Odorants
- Abstract
Electroencephalographical (EEG) recording studies have shown that odorants produce olfactory evoked potentials (OEPs) on the scalp surface. However, EEGs can only provide limited information about the intracerebral sources from where the OEPs are generated. By contrast, intracerebral EEG recordings enable direct examination of the electrophysiological activity from a given cerebral area. In the present study, neural activity was recorded from the amygdala of seven epileptic patients undergoing intracerebral EEG recordings prior to surgical treatment for relief of intractable seizures. Two olfactory tests were used: a passive-stimulation test consisting of the successive presentation of 12 common odorants and a suprathreshold detection test including both odorant and non-odorant stimulations. Recordings from the amygdala revealed that all odorant stimulations induced large and reproducible OEPs, whereas the non-odorant stimulations did not. It was also found that repetition of the same odorant stimulation led to a decrease in the latency of the first OEP component. This modulation, which corresponds to a faster olfactory processing, strongly suggests that the amygdala is involved in early olfactory attentional processes. In conclusion, it appears that the human amygdala discriminates the incoming information from the nasal airflow as being odorant or not and, additionally, that its speed of processing is sensitive to recent experience with an odor.
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- 2001
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19. Rating of different olfactory judgements in Alzheimer's disease.
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Royet JP, Croisile B, Williamson-Vasta R, Hibert O, Serclerat D, and Guerin J
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- Adult, Aged, Aged, 80 and over, Analysis of Variance, Cognition physiology, Cognition Disorders diagnosis, Cognition Disorders etiology, Female, Humans, Identification, Psychological, Judgment physiology, Male, Middle Aged, Odorants, Olfaction Disorders diagnosis, Recognition, Psychology physiology, Reference Values, Smell physiology, Alzheimer Disease complications, Alzheimer Disease physiopathology, Cognition Disorders physiopathology, Olfaction Disorders etiology, Olfaction Disorders physiopathology
- Abstract
Using simple successive tasks we assessed the influence of Alzheimer's disease on the processing of different odours. Fifteen patients with Alzheimer's disease, 15 old control subjects and 15 young control subjects were tested. The experiment included two sessions. Initially 12 odorants were presented, one odorant every minute. For each odour the subjects were asked to rate intensity, pleasantness, familiarity and edibility using linear rating scales. The odorants were then presented a second time and the subjects were asked to identify them. The results show that the intensity scores were lower in old control subjects and Alzheimer patients than in the young control subjects and that familiarity and identification scores were lower in Alzheimer patients than in old control and young control subjects. When we compared the five olfactory tasks the impairment of performance in Alzheimer patients was relatively higher for identification than familiarity, itself higher than the intensity judgement. No difference was observed between the three groups of subjects for pleasantness and edibility judgements.
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- 2001
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20. Functional neuroanatomy of different olfactory judgments.
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Royet JP, Hudry J, Zald DH, Godinot D, Grégoire MC, Lavenne F, Costes N, and Holley A
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- Adult, Brain Mapping, Cerebral Cortex diagnostic imaging, Dominance, Cerebral physiology, Emotions physiology, Female, Frontal Lobe blood supply, Frontal Lobe diagnostic imaging, Humans, Imaging, Three-Dimensional, Magnetic Resonance Imaging, Male, Mental Recall physiology, Regional Blood Flow physiology, Cerebral Cortex blood supply, Smell physiology, Tomography, Emission-Computed
- Abstract
Humans routinely make judgments about olfactory stimuli. However, few studies have examined the functional neuroanatomy underlying the cognitive operations involved in such judgments. In order to delineate this functional anatomy, we asked 12 normal subjects to perform different judgments about olfactory stimuli while regional cerebral blood flow (rCBF) was measured with PET. In separate conditions, subjects made judgments about the presence (odor detection), intensity, hedonicity, familiarity, or edibility of different odorants. An auditory task served as a control condition. All five olfactory tasks induced rCBF increases in the right orbitofrontal cortex (OFC), but right OFC activity was highest during familiarity judgments and lowest during the detection task. Left OFC activity increased significantly during hedonic and familiarity judgments, but not during other odor judgments. Left OFC activity was significantly higher during hedonicity judgments than during familiarity or other olfactory judgments. These data demonstrate that aspects of odor processing in the OFC are lateralized depending on the type of olfactory task. They support a model of parallel processing in the left and right OFC in which the relative level of activation depends on whether the judgment involves odor recognition or emotion. Primary visual areas also demonstrated a differential involvement in olfactory processing depending on the type of olfactory task: significant rCBF increases were observed in hedonic and edibility judgments, whereas no significant rCBF increases were found in the other three judgments. These data indicate that judgments of hedonicity and edibility engage circuits involved in visual processing, but detection, intensity, and familiarity judgments do not., (Copyright 2001 Academic Press.)
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- 2001
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21. Evidence for separate perceptive and semantic memories for odours: a priming experiment.
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Koenig O, Bourron G, and Royet JP
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- Adolescent, Adult, Female, Humans, Male, Memory, Odorants, Perception, Semantics, Smell
- Abstract
Sixty-four subjects participated in an olfactory priming experiment comprising separate study and test phases. Priming was measured within the olfactory modality (intramodal condition) and from the visual modality to the olfactory modality (intermodal condition). In the study phase of the intramodal condition, subjects were exposed twice to a series of odours: once performing a semantic orientation task (deciding which of seven categories odour stimuli belonged to) and once performing a perceptual orientation task (judging the intensity, the hedonicity and the familiarity of odour stimuli). Half of the odour stimuli corresponded to edible products, the other half did not. The study phase of the intermodal condition was similar, with the exception that the names of the odours (instead of the odours themselves) were presented. In the test phase, subjects were presented with primed and non-primed odour targets and had to decide as fast as possible whether the target corresponded to an edible product or not. Response times and types were recorded by a computer. The analysis of response times revealed a priming effect in the intramodal condition only. Results are discussed in terms of separate perceptual and semantic subsystems that store odour representations.
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- 2000
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22. Emotional responses to pleasant and unpleasant olfactory, visual, and auditory stimuli: a positron emission tomography study.
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Royet JP, Zald D, Versace R, Costes N, Lavenne F, Koenig O, and Gervais R
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- Adult, Brain diagnostic imaging, Brain Mapping, Data Display, Female, Humans, Male, Odorants, Pilot Projects, Reaction Time physiology, Reference Values, Stimulation, Chemical, Tomography, Emission-Computed, Acoustic Stimulation methods, Brain physiology, Emotions physiology, Photic Stimulation methods, Smell physiology
- Abstract
Neural correlates of responses to emotionally valenced olfactory, visual, and auditory stimuli were examined using positron emission tomography. Twelve volunteers were scanned using the water bolus method. For each sensory modality, regional cerebral blood flow (rCBF) during presentation of both pleasant and unpleasant stimuli was compared with that measured during presentation of neutral stimuli. During the emotionally valenced conditions, subjects performed forced-choice pleasant and unpleasant judgments. During the neutral conditions, subjects were asked to select at random one of a two key-press buttons. All stimulations were synchronized with inspiration, using an airflow olfactometer, to present the same number of stimuli for each sensory modality. A no-stimulation control condition was also performed in which no stimulus was presented. For all three sensory modalities, emotionally valenced stimuli led to increased rCBF in the orbitofrontal cortex, the temporal pole, and the superior frontal gyrus, in the left hemisphere. Emotionally valenced olfactory and visual but not auditory stimuli produced additional rCBF increases in the hypothalamus and the subcallosal gyrus. Only emotionally valenced olfactory stimuli induced bilateral rCBF increases in the amygdala. These findings suggest that pleasant and unpleasant emotional judgments recruit the same core network in the left hemisphere, regardless of the sensory modality. This core network is activated in addition to a number of circuits that are specific to individual sensory modalities. Finally, the data suggest a superior potency of emotionally valenced olfactory over visual and auditory stimuli in activating the amygdala.
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- 2000
23. Functional anatomy of perceptual and semantic processing for odors.
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Royet JP, Koenig O, Gregoire MC, Cinotti L, Lavenne F, Le Bars D, Costes N, Vigouroux M, Farget V, Sicard G, Holley A, Mauguière F, Comar D, and Froment JC
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- Adult, Analysis of Variance, Brain blood supply, Female, Humans, Male, Odorants, Olfactory Pathways diagnostic imaging, Regional Blood Flow, Semantic Differential, Tomography, Emission-Computed, Brain Mapping, Olfactory Pathways physiology, Smell physiology
- Abstract
The functional anatomy of perceptual and semantic processings for odors was studied using positron emission tomography (PET). The first experiment was a pretest in which 71 normal subjects were asked to rate 185 odorants in terms of intensity, familiarity, hedonicity, and comestibility and to name the odorants. This pretest was necessary to select the most appropriate stimuli for the different cognitive tasks of the second experiment. The second one was a PET experiment in which 15 normal subjects were scanned using the water bolus method to measure regional cerebral blood flow (rCBF) during the performance in three conditions. In the first (perceptual) condition, subjects were asked to judge whether an odor was familiar or not. In the second (semantic) condition, subjects had to decide whether an odor corresponded to a comestible item or not. In the third (detection) condition, subjects had to judge whether the perceived stimulus was made of an odor or was just air. It was hypothetized that the three tasks were hierarchically organized from a superficial detection level to a deep semantic level. Odorants were presented with an air-flow olfactometer, which allowed the stimulations to be synchronized with breathing. Subtraction of activation images obtained between familiarity and control judgments revealed that familiarity judgments were mainly associated with the activity of the right orbito-frontal area, the subcallosal gyrus, the left inferior frontal gyrus, the left superior frontal gyrus, and the anterior cingulate (Brodmann's areas 11, 25, 47, 9, and 32, respectively). The comestibility minus familiarity comparison showed that comestibility judgments selectively activated the primary visual areas. In contrast, a decrease in rCBF was observed in these same visual areas for familiarity judgments and in the orbito-frontal area for comestibility judgments. These results suggest that orbito-frontal and visual regions interact in odor processing in a complementary way, depending on the task requirements.
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- 1999
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24. A re-estimation of the number of glomeruli and mitral cells in the olfactory bulb of rabbit.
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Royet JP, Distel H, Hudson R, and Gervais R
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- Analysis of Variance, Animals, Image Processing, Computer-Assisted, Male, Mice, Rabbits, Rats, Rats, Wistar, Reproducibility of Results, Species Specificity, Olfactory Bulb cytology
- Abstract
Although morphological characteristics of mammalian olfactory bulb (OB) are well documented in rodents (rat, mice), only one study has been performed in rabbit, which is also commonly used in olfactory research. The study carried out by Allison and Warwick in 1949 led to surprising results still quoted in recent literature. The present study re-examined this question in young rabbit OB, while it was also conducted with rat as control. In five animals of each species, areas and coordinates of glomeruli and mitral profiles were measured in 10 coronal sections uniformly distributed along the rostrocaudal axis of the OB, and a distribution-free stereological method was used to compute values along this axis. For glomeruli, the estimated number was 4200 in rat and 6300 in rabbit. While this estimation matched with those found in literature in rat, it strikingly differed from the Allison and Warwick's value of 1900 only. For mitral cell number, we found 59,600 while the preceding study found 45,000 only. In contrast to the number of glomeruli, the mitral cell number in rat and rabbit were very close. Indeed, results showed 56, 200 in rat. The results suggest that the numbers of olfactory glomeruli and mitral cells has been previously underestimated in rabbit, and that the number of glomeruli changes as a function of species. In addition, both the results of the present study and reports in the literature suggest the number of mitral cells to be rather similar in mouse, rat and rabbit. As a consequence, the glomerular/mitral cell ratio is likely to varied in a wide range across species., (Copyright 1998 Elsevier Science B.V.)
- Published
- 1998
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25. Stereology: a method for analyzing images.
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Royet JP
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- Artifacts, Models, Neurological, Mathematics, Neurobiology methods
- Abstract
This review deals with notions of shape, sizes, numbers, densities and orientation in space, all basic concepts in stereology. With the initiation by Delesse in 1847, but mainly since the beginning of the XXth century, many stereological methods have been published allowing us to relate two-dimensional measurements easily obtainable on flat histological images with three-dimensional characteristics of the structure analysed. Looking at these methods, the neurobiologist, generally impermeable to concepts of sampling, statistical bias, efficiency, cost of effort and distribution-free, is discountenanced and continues old laboratory usages and customs. Furthermore, for the last ten years, the advent of a plethora of new powerful tools, considered as assumption-free and more efficient than the previous ones, increase the risk proportionately the disarray of the potential user. The purpose of this review is to present synthetically all traditional and actual aspects of stereology in order to guide the reader in the labyrinth of this speciality. The necessarily short exposition is compensated by many references to which the beginner or the initiated can refer.
- Published
- 1991
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26. Some effects of conditioned aversion on food intake and olfactory bulb electrical responses in the rat.
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Pager J and Royet JP
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- Animals, Apomorphine poisoning, Association, Electrophysiology, Hunger, Male, Odorants, Rats, Satiation, Avoidance Learning physiology, Conditioning, Operant physiology, Feeding Behavior physiology, Olfactory Bulb physiology, Taste physiology
- Abstract
Rats maintained on an unadulterated synthetic food, available from 8 a.m. to 10 a.m. everyday, were submitted to an aversive conditioning schedule on which a first ingestion of eucalyptol-flavored food (EF) was followed by an apomorphine injection (20 mg/kg, ip). In the first experiment the daily food intake was measured from Day 1 to 17, during the first and second hours of the meal. The EF was offered on Days 8, 9, and 17 during the first or the second hour of the meal (Series B or A). On Day 8, the meal was followed in a group of rats by the apomorphine injection. As compared with the intake of Day 8, the mean EF intake of Day 9 was significantly decreased in Series A and B, and of Day 17 in Series A only. No significant EF-intake modification could be observed in a saline-injected group or in an untreated control group. In the second experiment, rats bearing bulbar electrodes for the chronic recording of multiunit mitral cell responses received a 2-hr EF meal before the apomorphine injection. They were stimulated by puffs of odors of pure eucalyptol, unadulterated food, and EF and recorded in hungry and satiated states. Before the aversive conditioning, a significantly greater occurrence of positive responses to the odors of unadulterated food and EF was observed in hungry rats compared with satiated rats. The eucalyptol odor yielded equivalent patterns of responses in hungry and satiated rats before and after conditioning. Conditioning did not alter the modulated responses to unadulterated food odor (a greater occurrence of positive responses was still observed in hungry rats) but modified the responses to the odor of EF (the same high rate of positive responses was then observed in satiated and hungry rats). Electrophysiological data are discussed in terms of palatability changes and food-odor meaning.
- Published
- 1976
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27. An olfactometric cage suitable for short duration stimulations of unrestrained small animals.
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Vigouroux M and Royet JP
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- Animals, Equipment and Supplies, Methods, Odorants, Smell
- Abstract
An olfactometric cage was constructed in order to perform experiments on olfactory perception in unrestrained rats. Olfactory stimuli were measured with a flame ionization detector (FID). A cylindrical cage was divided into 2 compartments, an upper expansion chamber (B) and a lower stimulation chamber (A) with a perforated metal plate. Before entering B, the odours were diluted with purified air in a mixing chamber. The odorized air was then delivered to a small chamber on top of the cage and distributed by a thin slit surrounding B. A homogeneous distribution of holes in the metal plate provided a laminar flow characterized by a parabolic front. This flow was suitable for long duration exposures of animals of odours. However, for short stimulations (10 sec), variations of amplitude and delay were observed in the horizontal plane of the olfactometric cage. To flatten this parabolic front a new distribution of holes was calculated. A partially turbulent air flow was obtained in A. creating some fluctuations in the time-course of stimulations. However, the amplitude and the delay of the signals were reasonably constant in all parts of the cage floor. It is possible to use this apparatus for other purposes than those originally intended, e.g. behavioural studies.
- Published
- 1981
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28. Olfactory bulb responsiveness to an aversive or novel food odor in ;the unrestrained rat.
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Royet JP and Pager J
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- Animals, Food, Hunger, Learning, Male, Rats, Rats, Inbred Strains, Satiation, Odorants, Olfactory Bulb physiology, Smell
- Abstract
The mechanisms subserving neophobia and learned aversion have been investigated by recording multiunit olfactory bulb discharges either in hungry rats following food deprivation or in satiated rats. Under the two conditions, rats were stimulated with the smell of their familiar maintenance diet or that of a novel food or of control food-unrelated odor. Responses to the odor of the novel food were tested, following a pairing of the first or the second intake of that food with a LiCl injection, or following its first intake paired with a NaCl control injection. All rats exhibited enhanced level of discharges when they were stimulated in the hungry state with the smell of the familiar food and not when stimulated with the non-alimentary control odor. The hunger to satiety modulation of olfactory bulb discharges, also exhibited in rats tested with the smell of the novel food, previously paired with NaCl, was absent after a LiCl-induced taste aversion to this odor. The small, although significant, modulation observed when the conditioning of aversion occurred with the less novel food is consistent with the view that learned safety prevails upon learned harmfulness. Results are discussed in terms of relations of olfactory bulb electrical responses to odors with food palatability, neophobia and learned aversion.
- Published
- 1981
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29. Specificity of spatial patterns of glomerular activation in the mouse olfactory bulb: computer-assisted image analysis of 2-deoxyglucose autoradiograms.
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Royet JP, Sicard G, Souchier C, and Jourdan F
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- Animals, Autoradiography, Deoxyglucose metabolism, Hemiterpenes, Image Processing, Computer-Assisted, Mice, Odorants, Pentanoic Acids, Pentanols, Glucose metabolism, Olfactory Bulb physiology, Smell physiology
- Abstract
We have developed a computer-assisted method for analyzing the 2-deoxyglucose (2-DG) autoradiograms of mice olfactory bulbs. The purpose of the study was to numerize the maps of glomerular activation in order to achieve a statistical comparison of the glomerular patterns evoked by different stimuli. The spatial distribution of glomerular activation was displayed on unfolded representations of the glomerular layer which were built up using glomerular optical densities (OD) measured systematically within 13 sections per bulb. Each bulbar sample was converted into an 'OD profile'. A matrix composed of 18 OD profiles was submitted to a principal component analysis. The first factor which accounted for 28% of the variance separated unambiguously two clusters corresponding to the bulbs issued from animals stimulated with amylacetate and isovaleric acid, respectively. The second and third factors which accounted for 14% and 12% of the variance segregated the control group (animals exposed to pure air) from the odor-stimulated ones. It was demonstrated that the cluster separation was actually due to the specific spatial distribution of the most-labelled glomeruli. A particular attention was paid to the well-delineated glomerular activation evoked by isovaleric acid. The results demonstrate the specificity and reliability of the glomerular 2-DG patterns. The method should be useful for further comparisons of patterns elicited by larger sets of odorant compounds.
- Published
- 1987
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30. Effect of local 6-OHDA and 5,6-DHT injections into the rat olfactory bulb on neophobia and learned aversion to a novel food.
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Royet JP, Gervais R, and Araneda S
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- Animals, Discrimination Learning drug effects, Eating drug effects, Male, Medial Forebrain Bundle drug effects, Norepinephrine metabolism, Oxidopamine, Rats, Rats, Inbred Strains, Serotonin metabolism, Smell drug effects, 5,6-Dihydroxytryptamine pharmacology, Avoidance Learning drug effects, Hydroxydopamines pharmacology, Olfactory Bulb drug effects, Taste drug effects
- Abstract
This study investigated the role of the noradrenergic (NA) and the serotoninergic (5-HT) centrifugal fibers on the olfactory bulb (OB). For this purpose, behavioral responses largely depending on olfactory information were measured in rats after discrete bilateral injections into the OB with either 6-hydroxydopamine (6-OHDA) or 5,6-dihydroxytryptamine (5,6-DHT) solutions. The evaluation of the level of endogenous 5-HT in the OB was measured by high performance liquid chromatography. The NA fibers appeared lesioned after the 6-OHDA treatment only, whereas the 5-HT level was significantly reduced in 5,6-DHT treated bulbs only (34% of the control value). The animals were fed on usual stock-diet (S) and had the choice between S and a novel food (V) on neophobia test, and again on the following day after aversive conditioning (0.9 mEq/kg LiCl i.p.). The behavioral results showed that the drug administrations were followed by a significant effect in one case only: the intake of V was significantly enhanced in 6-OHDA treated animals during neophobia test. Thus, neophobia was reduced by 6-OHDA. The data are interpreted as follows: (1) the lesion of the bulbar NA or 5-HT fibers does not induce total anosmia; and (2) the NA innervation of the OB could play a role in the modulation of behavioral responses to biological odorants.
- Published
- 1983
- Full Text
- View/download PDF
31. Lesions of the olfactory pathways affecting neophobia and learned aversion differentially.
- Author
-
Royet JP and Pager J
- Subjects
- Animals, Chlorides poisoning, Dominance, Cerebral physiology, Eating drug effects, Lithium poisoning, Lithium Chloride, Male, Rats, Rats, Inbred Strains, Retention, Psychology physiology, Avoidance Learning physiology, Central Nervous System physiology, Olfactory Pathways physiology, Smell physiology, Taste physiology
- Abstract
The contribution of ascending olfactory pathways in neophobia and learned aversion to the same food was investigated in male rats bearing lesions of both olfactory peduncles, or one olfactory peduncle and the opposite lateral olfactory tract or anterior limb of the anterior commissure. The animals were fed on usual stock diet (S) offered as a choice with novel vanilla food (V) on test days: during neophobia (N), then before and after aversive conditioning (Aa, At). Daily food intake was measured, and the preference was expressed as V/(V + S). Experiment 1 included a neophobia test, before aversive conditioning (3 mEq/kg LiCl, i.p.). In Experiment 2, aversion only was studied (0.9 mEq/kg). In the neophobia test, the preference ratio was 7% in unoperated controls, and 43-52% in the 3 lesioned groups. The same controls had preference ratios equal to 64% and 22%, before and after aversive learning. Similar drops were observed for any lesioned group in Expt. 1. The decrease was less obvious, although significant, in rats of Expt. 2 with asymmetric lesions; those with both olfactory peduncles cut through maintained the same preference ratio (48%) before and after LiCl treatment. The data are interpreted assuming that: (1) lateral olfactory tract and anterior commissure both contribute to information processing in neophobia and aversion; (2) olfactory cues subserve neophobia prepotently; and (3) one cannot account for the sensory determinism of neophobia and aversion calling for a single mechanism.
- Published
- 1982
- Full Text
- View/download PDF
32. Neophobic behavior to food and electrical responses of olfactory bulb in rat.
- Author
-
Royet JP and Pager J
- Subjects
- Animals, Evoked Potentials, Habituation, Psychophysiologic physiology, Hunger physiology, Male, Neurons physiology, Rats, Satiation physiology, Smell physiology, Feeding Behavior physiology, Olfactory Bulb physiology
- Published
- 1980
- Full Text
- View/download PDF
33. A comparative study of 2-deoxyglucose patterns of glomerular activation in the olfactory bulbs of C57 BL/6J and AKR/J mice.
- Author
-
Sicard G, Royet JP, and Jourdan F
- Subjects
- Action Potentials, Animals, Hemiterpenes, Mice, Mice, Inbred AKR physiology, Mice, Inbred C57BL physiology, Olfactory Bulb physiology, Deoxy Sugars metabolism, Deoxyglucose metabolism, Mice, Inbred AKR metabolism, Mice, Inbred C57BL metabolism, Olfactory Bulb metabolism, Pentanoic Acids, Pentanols, Valerates
- Abstract
We have studied the patterns of glomerular activation evoked in the olfactory bulbs of C57BL/6J and AKR/J mice by olfactory stimulations with amyl acetate or isovaleric acid. Patterns of glomerular activation were obtained with the 2-deoxyglucose method and subsequently compared using a computer-assisted image analysis. The mice of both inbred strains stimulated with amyl acetate or isovaleric acid were characterized by areas of high 2-deoxyglucose uptake in their glomerular layer. Statistical comparisons of the patterns demonstrated that they were odor-specific. Although C57BL/6J mice have been found to be specifically anosmic to isovaleric acid, our findings indicate that their olfactory system is topographically activated by olfactory stimulations with this odorant. However, patterns of glomerular activity evoked by isovaleric acid stimulations are not similar in the two studied strains.
- Published
- 1989
- Full Text
- View/download PDF
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