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The influenceof maturation medium, serum and hormonal supplements, type of bovine oocyte, as well as the co-culture with granulosa cells for in vitro bovine oocytes maturation on the in vitro fertilization rate were investigated. Immature oocytes with cumulus cells were cultured with two different maturation media (M-199 and DM EM; Experiment 1). In Experiment 2, in vitro maturation was performed with two serum supplements and one hormonal supplement (FCS. ECS and PMSG+HCG). In Experiment 3. on vitro maturation was performed with two different bovine oocyte types (CEO and CDC) In Experiment 4. in vitro maturation was performed with or without the addition of granulosa cells. After in vitro oocyte maturation, oocytes were fertilized using frozen-thawed semen which was previously capacitated with bovine serum albumin and heparin for 15 minutes. The M-199 showed a fertilization rate higher (55 percent; p

Three experiments were carried out to investigate whether cumulus cells, Bovine Follicular Fluid (BFF) and granulosa cells, when added as supplements to the maturation medium, support the in virto maturation of immature bovine oocytes. In the first experiment, the maturation medium was supplemented with three different concentrations of the BF F (10, 20 and 50 p.100, respectively). In the second experiment, the effect of three different concentrations of the granulosa (1,10 y 100x 106 cells/ml, respectively), on in vitro oocyte maturation porcentages were evaluated. In the third experiment, the effect of the presence or absence of cumulus cells on the in vitro oocyte maturation rates was compared. Maturation rates at the metaphase II stage, following supplementation with 20 p.100 BFF (60 p.100) were significantly higher (p"O.05) than those obtained in control group (45 p.100). Significant differences between the 10 p.100 BFF, 20 p.100 BFF and 50 p.100 BFF treatments, were not observed. ml and the control treatments (90 p.100 and 85 p.100, respectively) were significantly higher (pa0.001) than when 10 and 10000" cells/ml (66 p.100 and 47 p.100, respectively) were added. Significant differences in the presence (78 p.100) or absence (62 p.100) of cumulus cells (p

In a sample of Retinto Beef Cattle under control of a breeding program in Andalusia, the average current inbreeding coefficient has been estimated. Ancestral information on 492 dams and 25 sires was analysed. The average current inbreeding coefficient (F) calculaled for the whole sample was 1.52 p.100 but the pedigree information was very incomplete. Taking into consideration only the females with information equal or greater than 25 p.100 in the third ancestral generation, the current inbreeding coefficient (F) ranged from 2.06 p,100 to 3.32 p.100 with information percentage at 100 p.100. The estimated F value inthese animals compared with those values obtained in other beef breeds is the highest at the present time, only being comparable to those reported by Kidd et al. (1980) for the Iberian Mertolenga and de Lidia breed. This work confirmed Kidd"s hypothesis (1980) concerning the great F value calculated in this breed. This is explained by breeding practices and inbreeding from mating dosely related animals (12.2 p.100 females showed a value of F>0 and 3-9 p. 100 showed F=25 p.100) Further work must be done in this field to estimate the current inbreeding coefficient of the breed which appears extremely high and to establish a mating policy. Se ha estimado la media del coeficiente actual de consanguinidad en una muestra de ganado vacuno de carne de raza Retinta. Esta muestra consiste en los animales bajo control del plan de mejora llevado a cabo en poblaciones andaluzas. Se han analizado genealogías de 492 vacas madres y 25 sementales. La media calculada del coeficiente actual de consanguinidad (F) para la muestra es F=1,52 p-100, pero hay que tener en cuenta que la información genealógica era muy incompleta. Considerando sólo las hembras que cuentan con una información en la tercera generación igual o mayor al 25 p-100, el valor de F tiene un rango entre 2,06 y 3,32 p-100 cuando el porcentaje de información es del 100 p.100. El valor estimado de la F es muy elevado si lo compararnos con los obtenidos para otras razas de carne, siendo comparable sólo con los valores calculados por Kidd et al. (1980) para las razas ibéricas Meridenga, Toro de Lidia y Retinto. Este trabajo confirma las hipótesis de Kidd et al. (1980) sobre el alto valor de F estimado por ellos en el ganado Retinto. Este elevado valor podría explicarse por la costumbre de los criadores de ulilizar un reducido número de sementales de la propia ganadería y al cruzamiento de individuos estrechamente emparentados (12,2 p.100 de las vacas madres analizadas presentaban un valor de F>0 y el 3,2 p.100 de F=25 p.100). Esos resultados señalan la necesidad de ampliar estos estudios a un mayor número de individuos de la raza para estimar el coeficiente de consanguinidad que parece ser extremadamente alto, asi como establecer una política de cruzamientos.

The weekly milkyield of72 canarygoat breeding group raised on the experimental farm of the Centro de Investigación y Tecnología Agraria (Tenerife. Canary Islands) was recorded. All does were in their first lactation and were selected from the most representative local farms. During the control day does were hand milked once a day starting one week after kidding until the 32" week of lactation. Milk yield increased from 2.4 kg in the first control to milk yield peak (2.5 kg) in the fourth week, then decreased gradually and it was found that at the 32" week the milk yield was 1.4 kg. To fit our data the function proposed by Cooby and Le Du (1978) had the smaller error mean square. y-=a( I -e-h")-cx Han sido controladas las producciones de 72 cabras de la Agrupación Caprina Canaria mantenidas en condiciones experimentales en el Centro de Investigación y Tecnología Agraria (Tenerife. Islas Canarias). Todas fas cabras se encontraban en su primera lactación y fueron selecionadas en las granjas más representativas de las islas. Durante el control las cabras fueron ordeñadas a mano una vez, al día, comenzando una semana después del parto hasta la 32 semana de lactación. La producción se incrementó de 2,4 kg en el primer control hasta alcanzar el pico de producción (2,5 kg) en la cuarta semana, entonces decreció gradualmente hasta de 1,4 kg en el control de la semana 32. Con el ajuste de nuestros datos a diversas funciones, el modelo propuesto por Coobyy Le Du (1978) ofreció el valor más pequeño del cuadrado medio residual.

In this paper an evaluation in dollars of the cost of the Andalusian rare indegenaus breeds conservation by several methods is presented. It constitutets a preliminary aproach to the development of conservation and preservation planes from the official and privates institutions. In this study we include seven cattle breeds, fo+ir sheep breeds, and three goat breed. En este trabajo se presenta una evaluación en dólares del coste de la conservación por diversos métodos de las raza minoritarias indígenas Andaluzas. Esto constituye una primera aproximación a el desarrollo de planes de conservación ypreservación desde las instituciones públicas y privadas. En este estudio incluimos siete razas bovinas, cuatro razas ovinas y tres caprinas.

An intersex goat of Malagueña breed with a karyotype which is clearly different from that of the rest of the species as regards number and shape was studied. The karyotype was formed by 59 chromosomes all of them acrocentric with the exception of one large metacentric chromosome. The morphometric study of the latter showed us that it was the result of a robertsonian translocation between chromosomes of pairs 10 and 12. Se ha estudiado una cabra intersexo de la raza Malagueña con un cariotipo diferente del de la especie en cuanto a número y forma. Este se encontraba formado por 59 cromosomas, todos ellos acrocéntricos con la excepción de un gran cromosoma metacéntrico. El estudio morfométrico del mismo nos indicó ser el fruto de una translocación robertsoniana entre cromosomas de los pares 10 y 12.