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Poster.-- 4th Meeting of Medicinal Biotechnology, Porto, May 17th 2019, Streptococcus includes numerous mastitis causing species, being responsible for high economic losses as well as human health issues Recently, new rapid molecular microbial diagnostic methods based on genomics and proteomics have been developed in order to achieve faster and more effectively species identification than classical and culture based methods which are labour intensive Liquid chromatography electrospray ionization tandem mass spectrometry (LC ESI MS/MS) has been used for the analysis of bacterial pathogen strain specific diagnostic peptides 1 2 As bacteriophages are high specific to their host pathogens, their nucleic acids, antibodies, phage display peptides ( and most recently phage’s receptor binding proteins ( have been studied as biosensor for pathogen detection 3 4 In addition, LC ESI MS/MS techniques have been employed for the identification and detection of bacterial bacteriophages 5 However, any study has been published for Streptococcus phage detection and identification by LC MS MS so far, This work was funded by the Spanish Ministry of Economy and Competitivity Project AGL 2013 48244 R and by the European Regional Development Fund ( 2007 2013 Ana Gonzalez Abril pre doctoral scholarships was funded by Xunta de Galicia and the European Union (European Social Fund ESF)