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Objective This study evaluates goat sperm motility in response to metabolic substrates and various inhibitors, aiming to assess the relative contribution of glycolysis and mitochondrial oxidation for sperm movement and adenosine triphosphate (ATP) production. Methods In the present study, two main metabolic substrates; 0 to 0.5 mM glucose and 0 to 30 mM pyruvate were used to evaluate their contribution to sperm movements of goats. Using a 3-chloro-1,2-propanediol (3-MCPD), a specific inhibitor for glycolysis, and carbonyl cyanide 3-chlorophenylhydrazone as an inhibitor for oxidative phosphorylation, cellular mechanisms into ATP-generating pathways in relation to sperm movements and ATP production were observed. Data were analysed using one-way analysis of variance for multiple comparisons. Results Sperm motility analysis showed that either glucose or pyruvate supported sperm movement during 0 to 30 min incubation. However, the supporting effects were abolished by the addition of a glycolysis inhibitor or mitochondrial uncoupler, concomitant with a significant decrease in ATP production. Although oxidative phosphorylation produces larger ATP concentrations than those from glycolysis, sperm progressivity in relation to these two metabolic pathways is comparable. Conclusion Based on the present study, we suggest that goat sperm use glucose and pyruvate to generate cellular energy through glycolysis and mitochondrial respiration pathways to maintain sperm movement.

ABSTRACT. Sapi perah yang berproduksi tinggi membutuhkan ransum berenergi tinggi dan asam lemak esensial. Penambahan lemak dalam ransum dapat mengatasi kekurangan energi dan asam lemak esensial. Permasalahan umum dari pemberian lemak atau minyak pada dosis tertentu dapat mengganggu ekosistem rumen, sehingga dalam pemberiannya perlu diproteksi. Ruminer adalah suplemen komersial berupa minyak sawit yang diproteksi mineral kalsium. Penelitian ditujukan untuk mengetahui penambahan ruminer dalam ransum lengkap terhadap produksi susu 4% FCM dan produksi nutrien susu sapi perah. Penelitian dilakukan secara eksperimental terhadap 10 ekor sapi perah Friesian Holstein pada masa laktasi sama. Sapi tersebut dibagi dua, masing-masing lima ekor. Perlakuan berupa ransum tanpa ditambah ruminer (P0) dan ransum ditambah 250 gram ruminer.ekor-1.hari-1 (P1). Peubah yang diamati adalah produksi susu 4% FCM diukur setiap hari selama 60 hari dan kualitas susu diukur setiap 10 hari. Hasil penelitian menunjukkan bahwa produksi susu 4% FCM, produksi total padatan, lemak, protein dan laktosa susu pada sapi diberi ruminer lebih tinggi (P0,05) daripada sapi yang tidak diberi ruminer. Disimpulkan bahwa pemberian ruminer meningkatkan produksi susu 4% FCM, total padatan, lemak, protein dan laktosa susu. Pemberian ruminer sebanyak 250 gram.ekor-1hari-1 mampu meningkatkan 2 kg produksi susu 4% FCM pada sapi perah laktasi. (Effects of ruminer addition in dairy cattle rations on milk 4% FCM and nutrient production) ABSTRAK. High-yielding dairy cows require high-energy rations and essential fatty acids. The addition of fat in the ration can overcome the lack of energy and essential fatty acids. The general problem of giving fat or oil at certain doses can disrupt the rumen ecosystem, so that it needs to be protected. Ruminer is a commercial supplement in the form of calcium mineral protected palm oil. This research was aimed to determine the addition of ruminants in complete rations on the production of 4% FCM milk and the nutrient production of dairy cow milk. The research was carried out experimentally on 10 dairy cows during the same lactation period. The cow is divided into two, five each. The treatments were rations without adding ruminer (P0) and rations added by 250 grams of ruminer. head-1 day-1 (P1). The variables observed were 4% FCM milk production measured every day for 60 days and milk quality was measured every 10 days. The results showed that the production of 4% FCM milk, the total production of solids, fat, protein and milk lactose in cows fed with ruminer was higher (P 0.05) than cows that were not given ruminer. It was concluded that the provision of ruminer increased milk production by 4% FCM, total solids, fat, protein and milk lactose. Giving a ruminer of 250 grams of head-1.day-1 was able to increase 2 kg of 4% FCM milk production in lactating dairy cows.

Abstract Lemna minor is a water plant that is easily obtained and has a good nutrient content that is high in protein. High protein for livestock can be used for production purposes. The study aims to determine the effect of administration of lemna minor on fat content, specific gravity, and dry matter without fat milk of friesien holstein dairy cows. The object of the study was 20 second-to-four-month FH lactating cows in the Dairy Farmer Group of Kp Tanggung Renteng Village, Pamegatan Village, Cikajang District, Garut Regency, West Java Province, then kept for 60 days and given feed according to treatment, namely R0 = Grass 60% + Concentrate 40%, R1 = 50% Grass + 40% Concentrate + 10% wet Lemna, R2 = 60% Grass + 37% Concentrate + 3% Dry Lemna, R3 = 50% Grass + 37% Concentrate + 10% Wet Lemna + 3% Dry Lemna, R4 = Grass 45% + Concentrate 38% + Wet Lemna 15% + Dry Lemna 2%. The results showed that the effect of administering lemna minor did not affect on fat content, specific gravity, and dry matter without milk fat. The conclusion that administration of lemna minor in dairy cattle feed cannot increase fat content, specific gravity, and dry ingredients without fat Holstein friesien milk. Keywords : lemna minor, fat content, density, solid non fat Abstrak Lemna minor merupakan tanaman air yang mudah diperoleh dan memiliki kandungan nutrient yang baik yaitu protein tinggi. Protein yang tinggi bagi ternak dapat dimanfaatkan untuk meningkatkan produksi. Penelitian bertujuan untuk mengetahui pengaruh pemberian lemna minor terhadap kadar lemak, berat jenis, dan bahan kering tanpa lemak susu sapi perah friesien holstein. Objek penelitian yang digunakan adalah 20 ekor sapi FH laktasi kedua sampai empat bulan di Kelompok Peternak Sapi Perah Kp Tanggung Renteng Desa Pamegatan Kecamatan Cikajang Kabupaten Garut Provinsi Jawa Barat kemudian dipelihara selama 60 hari dan diberikan pakan sesuai dengan perlakuan yaitu R0= Rumput 60% + Konsentrat 40%, R1= Rumput 50% + Konsentrat 40% + Lemna basah 10%, R2= Rumput 60% + Konsentrat 37% + Lemna Kering 3%, R3= Rumput 50% + Konsentrat 37% + Lemna Basah 10% + 3% Lemna Kering, R4= Rumput 45% + Konsentrat 38% + lemna basah 15% + Lemna Kering 2%. Hasil penelitian menunjukkan bahwa pengaruh pemberian lemna minor tidak berpengaruh terhadap (P>0,05) kadar lemak, berat jenis, dan bahan kering tanpa lemak susu. Kesimpulan bahwa pemberian lemna minor dalam pakan sapi perah tidak dapat meningkatkan kandungan lemak, berat jenis, dan bahan kering tanpa lemak susu friesien Holstein. Kata kunci : lemna minor, kadar lemak, berat jenis, bahan kering tanpa lemak