Search

Your search keyword '"Réhault-Godbert, Sophie"' showing total 129 results
Start Over Author "Réhault-Godbert, Sophie" Language english
129 results on '"Réhault-Godbert, Sophie"'

9. Millifluidic Sensor Designed to Perform the Microwave Dielectric Spectroscopy of Biological Liquids

Author

Kozhemyakin, Yegor, Réhault-Godbert, Sophie, Dubuc, David, Grenier, Katia, Équipe Micro et nanosystèmes HyperFréquences Fluidiques (LAAS-MH2F), Laboratoire d'analyse et d'architecture des systèmes (LAAS), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT), Biologie des Oiseaux et Aviculture (BOA), Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Europe, projet européen PPILOW n°816172, and European Project: 816172,H2020-EU.3.2.1.1. - Increasing production efficiency and coping with climate change, while ensuring sustainability and resilience ,PPILOW (2019)

Subjects
liquids, [SPI.ELEC]Engineering Sciences [physics]/Electromagnetism, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, coplanar waveguide, transmission coefficient, millifluidic channel, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, microwave spectroscopy
Abstract

International audience; This paper presents a microwave sensor composed of one millifluidic channel that operates over a frequency range from 1 GHz to 4 GHz and is used for microwave spectroscopy of liquids with millilitre volumes. The accuracy of the device is first evaluated with BSA solutions of different concentrations and then with complex biological liquids such as hen egg yolk and albumen samples. Low standard deviations of the measured values of modulus and phase contrasts of the transmission coefficient S21 are obtained. These values correspond to 0.02 and 0,18 respectively for BSA solutions, as well as 0.05 and 0.03 for 10 samples of hen egg yolk and white respectively over the selected frequency range. Such accuracies enable the use of such a sensor for various applicative domains, including agronomy for which the investigation of the dielectric properties of eggs may be of interest for quality, freshness or status discrimination purpose.

Published
2022

10. The PPILOW project: Innovations improving welfare in low input and organic pig and poultry farms

Author

Collin, Anne, Meloni, Giuditta, Bonnefous, Claire, Re, Martina, van Vooren, Laura, Niemi, Jarkko, Väre, Minna, Lähtinen, Katja, Tuyttens, Frank A.M., Graat, Evelien, Vanden Hole, Charlotte, Rodenburg, Tb (bas), Kliphuis, Saskia, Giersberg, Mona, Tavares, Olivia, Desaint, Brieuc, Steenfeldt, Sanna, Pedersen, Lene, Engberg, Ricarda M., Almadani Mohamad, Isam, Carelli, Riccardo, Sciarretta, Marlene, Guilloteau, Laurence, Réhault-Godbert, Sophie, Gautron, Joël, Le Bihan-Duval, Elisabeth, Mignon-Grasteau, Sandrine, Berri, Cécile, Guettier, Elodie, Baéza, Elisabeth, Chartrin, Pascal, Bordeau, Thierry, Raynaud, Emilie, Couroussé, Nathalie, Cailleau-Audouin, Estelle, Crochet, Sabine, Tourneur, Léa, Guichaoua, Adrien, van den Brand, Henry, Castellini, Cesare, Reverchon, Maxime, Sourdioux, Michel, Akakpo, Roland, Rangel Pedersen, Ninfa, Schepens, Rene, Almind, Maria, Grenier, Katia, Dubuc, David, Le Lann, Marie-Véronique, Ponzio, Raffaella, Mainardi, Marina, Accotto, Caterina, Coletta, Monica, Guesdon, Vanessa, Leruste, Hélène, Billiard, Bérangère, Ferreira, Vitor Hugo Bessa, Baldinger, Lisa, Pluschke, Helen, Delanoue, Elsa, Laura, Warin, Pertusa, Marion, Stomp, Mathilde, Travel, Angelique, Bouvarel, Isabelle, Germain, Karine, Ravon, Laure, Calandreau, Ludovic, Labas, Valérie, Prunier, Armelle, Merlot, Elodie, Tallet, Céline, Perruchot, Marie-Hélène, Louveau, Isabelle, van Milgen, Jaap, Dessauge, Frederic, Clouard, Caroline, Lebret, Bénédicte, Montagne, Lucile, Faure, Justine, Zuliani, Anna, Venezia, Pietro, Canario, Laurianne, Ferchaud, Stéphane, Cozma, Vasile, Spinu, Marina, Horia Bǎieş, Mihai, Courboulay, Valérie, Roguet, Christine, Gaudré, Didier, Chevillon, Patrick, Alibert, Laurent, Decruyenaere, Virginie, Wavreille, José, Vanggaard, Pia, Vanggaard, Jan, Micheloni, Cristina, Thobe, Petra, Lombard, Sarah, Caillaud, Laura, Bernardet, Nelly, Collet, Julie, Molenaar, Roos, Mattioli, Simona, Hill, Nigel, Cadudal, François, Quentin, Maxime, Leterrier, Christine, Teixeira-Gomes, Ana-Paula, Uzbekova, Svetlana, Maugrion, Emilie, Rocchi, Lucia, Biologie des Oiseaux et Aviculture (BOA), Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Università degli Studi di Perugia = University of Perugia (UNIPG), Associazione Italiana per l'Agricoltura Biologica (AIAB), Scuela Santa Anna (SSSA), Scuola Universitaria Superiore Sant'Anna [Pisa] (SSSUP), BioForum Vlaanderen, Natural Resources Institute Finland (LUKE), Research Institute for Agricultural, Fisheries and Food (ILVO), Utrecht University [Utrecht], Institut Technique de l'Agriculture Biologique (ITAB), Aarhus University [Aarhus], Thuenen Institute of Farm Economics, European Association for Animal Production (EAAP), Les instituts techniques agricoles (Acta), Wageningen University and Research [Wageningen] (WUR), Syndicat des Sélectionneurs Avicoles et Aquacoles Français (SYSAAF), Fermentation Experts, Équipe Micro et nanosystèmes HyperFréquences Fluidiques (LAAS-MH2F), Laboratoire d'analyse et d'architecture des systèmes (LAAS), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT), Équipe DIagnostic, Supervision et COnduite (LAAS-DISCO), Slow Food Biodiversity, JUNIA (JUNIA), Université catholique de Lille (UCL), Thuenen-Institute of Organic Farming, ITAVI, Elevage Alternatif et Santé des Monogastriques (UE EASM), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Physiologie, Environnement et Génétique pour l'Animal et les Systèmes d'Elevage [Rennes] (PEGASE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-INSTITUT AGRO Agrocampus Ouest, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), SIVtro VSF Italia, Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-École nationale supérieure agronomique de Toulouse (ENSAT), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité Expérimentale Elevages Porcins Innovants (GenESI), USAMV Cluj-Napoca, Institut du Porc (IFIP), Centre Wallon de Recherches Agronomiques (CRA-W), Vanggaard Staldmontage, Harper Adams University, EAAP, PPILOW, and European Project: 816172,H2020-EU.3.2.1.1. - Increasing production efficiency and coping with climate change, while ensuring sustainability and resilience ,PPILOW (2019)

Subjects
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences, [SCCO]Cognitive science, [SDV.GEN]Life Sciences [q-bio]/Genetics, [SDV.BA]Life Sciences [q-bio]/Animal biology, [SDV.BDD]Life Sciences [q-bio]/Development Biology, [SHS]Humanities and Social Sciences
Abstract

International audience; The PPILOW project aims to co-construct innovations to improve Poultry and Pig Welfare in Low-input outdoor and Organic farming systems through a multi-actor approach. PPILOW implements a participatory approach for proposing and studying welfare-improvement levers. It will provide a combination of practical solutions that can be applied at a pan-European level with specific adjustments depending on citizen’s expectations and the target market. The multi-actor approach consists in involving end-users including farmers, breeding companies, feed producers, consumer associations, retailers, advisers, processors, and scientists in National Practitioner Groups (NPG) in six participating countries. PPILOW partners facilitate the groups by connecting NPG at European level, transferring scientific information, interacting with partners engaged in animal experiments, and co-creating innovations rising from NPG-specific demands. They co-build with PPILOW partners welfare self-assessment tools (development of the PIGLOW app for pigs and refinement of the EBENE® app for poultry), and innovative breeding, feeding, and rearing strategies and techniques to improve the welfare of animals. They co-design protocols, test innovations on farm, and disseminate the results. In turn, they receive insights on methods and scientific results, and inputs from other NPG reinforcing the value of the expected outcomes. Approaches focus on avoiding physical damage and the elimination of layer male chicks, on reducing boar taint of intact male pigs, promoting positive behaviours, animal health, and robustness through field studies with pigs and poultry. Multicriteria analyses of the most effective levers of welfare improvement will be performed to evaluate their economic, social, and environmental impacts based on the ‘One Welfare’ concept; economic and business models will also be developed. To ensure the rapid uptake of the project results by end-users, the close involvement of PPILOW’s NPG throughout the EU will ensure disseminationactivities and the facilitation of change. The PPILOW project has received funding from the European Union’s Horizon 2020 Research and Innovation Programme under grant agreement N°816172. www.ppilow.eu.

Published
2022

14. Structural modifications of the chorioallantoic membrane and the inner eggshell during chicken embryonic development

Author

Halgrain, Maëva, Bernardet, Nelly, Georgeault, Sonia, Hincke, Maxwell, Réhault-Godbert, Sophie, REHAULT-GODBERT, Sophie, Biologie des Oiseaux et Aviculture (BOA), Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Plateforme IBISA de Microscopie Electronique [CHRU de Tours] (UNIV Tours), Centre Hospitalier Régional Universitaire de Tours (CHRU Tours)-Université de Tours (UT), University of Ottawa [Ottawa], LE STUDIUM (LE STUDIUM), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de recherche pour le développement [IRD] : UR-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio]
Abstract

International audience; In oviparous species, the egg contains protective systems and provides energy and nutrients that support the development of the embryo until hatching. During embryonic development, extraembryonic structures are vital to assimilate egg nutrients, to protect the embryo, to ensure gaseous exchange and to accumulate metabolic waste. The chorioallantoic membrane (CAM), which develops in close contact with the inner eggshell from day 5 of incubation, is a highly vascularized structure. The CAM is located between the eggshell membranes (ESM, outer side) and the allantoic fluid that collects embryonic waste (inner side). The CAM was reported to be involved in decalcification of the inner eggshell and mineral transport from the eggshell to the embryo (for mineralization of its skeleton), in acid-base homeostasis, and in ion and H2O reabsorption from the allantoic fluid. To decipher the kinetics of its structural maturation, we compared the structure of the inner and outer surfaces of the CAM by electron microscopy. In parallel, ESM and the eggshell were analyzed to better appreciate the impact of CAM development on its surrounding structures. Eggs from Ross 308 broiler hens were incubated for 11 or 15 days (ED11 vs ED15, early and mature stage of CAM development, respectively). At each time point, eggs were broken, and the eggshell, ESM and the CAM were gently separated for analysis by scanning electron microscopy. Images corresponding to the cross-section and the inner side of the eggshell, eggshell membranes (ESM, outer and inner sides) and CAM (outer and inner sides) were obtained. At ED15, results showed that the inner side of the eggshell is degraded (decalcification) during embryonic development while the mammillary knobs of the eggshell remain tightly attached to the external membrane of the ESM. The CAM also undergoes major structural changes with some villous cells that emerge from its outer surface to reach the eggshell membrane and the eggshell. The molecular components associated with eggshell decalcification and mineral transport from the eggshell to the embryo via CAM’s vasculature remain essentially unknown. This study has been recently complemented by a transcriptomic analysis of the CAM (RNA sequencing) at these two developmental stages. Altogether, we believe that the combined findings of this work will lead to better understanding of CAM functions that are vital for embryonic development and skeletal mineralization during incubation.

Published
2022

15. Proteome and biological functions of inner and outer perivitelline layers in the chicken egg

Author

Brégeon, Mégane, Tomas, Daniel, Bernay, Benoit, Chessé, Magali, Zatylny-Gaudin, Céline, Labas, Valérie, Réhault-Godbert, Sophie, Guyot, Nicolas, Guyot, Nicolas, Biologie des Oiseaux et Aviculture (BOA), Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Plate-forme Phénotypage par imagerie in/ex vivo de l'Animal à la Molécule (Plate-forme PIXANIM), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Plateforme Proteogen, SFR ICORE 4206, Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Normandie Université (NU), Biologie des Organismes et Ecosystèmes Aquatiques (BOREA), and Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio]
Abstract

International audience

Published
2022

16. Welfare issues and potential solutions for laying hens in free range and organic production systems : a review based on literature and interviews

Author

Bonnefous, Claire, Collin, Anne, Guilloteau, Laurence A, Guesdon, Vanessa, Filliat, Christine, Réhault-Godbert, Sophie, Rodenburg, T Bas, Tuyttens, Frank A M, Warin, Laura, Steenfeldt, Sanna, Baldinger, Lisa, Re, Martina, Ponzio, Raffaella, Zuliani, Anna, Venezia, Pietro, Väre, Minna, Parrott, Patricia, Walley, Keith, Niemi, Jarkko K, Leterrier, Christine, AISS Animal Welfare, Gebhardt-Henrich, Sabine G., Biologie des Oiseaux et Aviculture (BOA), Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), JUNIA (JUNIA), Université catholique de Lille (UCL), VETOPOLE 26, Utrecht University [Utrecht], Ghent University Hospital, Instituut voor Landbouw en Visserijonderzoek, ITAVI, Aarhus University [Aarhus], Thuenen-Institute of Organic Farming, Associazione Italiana per l'Agricoltura Biologica (AIAB), Slow Food Italy, Slow Food International, Veterinari Senza Frontiere Italia, Sede c/o Istituto Zooprofilattico Sperimentale delle Venezie viale dell'Università, Natural Resources Institute Finland (LUKE), Harper Adams University, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), The PPILOW project has received funding from the European Union's Horizon 2020 research and innovation program under grant agreement N°816172., European Project: 816172,H2020-EU.3.2.1.1. - Increasing production efficiency and coping with climate change, while ensuring sustainability and resilience ,PPILOW (2019), and AISS Animal Welfare

Subjects
free range, General Veterinary, HELMINTH, HOUSING SYSTEM, pullet, [SDV]Life Sciences [q-bio], poultry, organic, health, POULTRY RED MITE, FERMENTED FEED, KEEL BONE-FRACTURES, veterinary(all), hen, GROWTH-PERFORMANCE, welfare, DERMANYSSUS-GALLINAE, INFECTIONS, EGG, QUALITY, Veterinary Sciences, FEATHER-PECKING BEHAVIOR, ENVIRONMENTAL ENRICHMENT
Abstract

International audience; In free-range and organic production systems, hens can make choices according to their needs and desires, which is in accordance with welfare definitions. Nonetheless, health and behavioral problems are also encountered in these systems. The aim of this article was to identify welfare challenges observed in these production systems in the EU and the most promising solutions to overcome these challenges. It is based on a review of published literature and research projects complemented by interviews with experts. We selected EU specific information for welfare problems, however, the selected literature regarding solutions is global. Free range use may increase the risk of infection by some bacteria, viruses and parasites. Preventive methods include avoiding contamination thanks to biosecurity measures and strengthening animals' natural defenses against these diseases which can be based on nutritional means with new diet components such as insect-derived products, probiotics and prebiotics. Phytotherapy and aromatherapy can be used as preventive and curative medicine and vaccines as alternatives to antibiotics and pesticides. Bone quality in pullets and hens prevents keel deviations and is favored by exercise in the outdoor range. Free range use also lead to higher exposure to variable weather conditions and predators, therefore shadow, fences and guard animals can be used to prevent heat stress and predation respectively. Granting a free range provides opportunities for the expression of many behaviors and yet many hens usually stay close to the house. Providing the birds with trees, shelters or attractive plants can increase range use. Small flock sizes, early experiences of enrichment and personality traits have also been found to enhance range use. Severe feather pecking can occur in free range production systems, although flocks using the outdoor area have better plumage than indoors. While many prevention strategies are facilitated in free range systems, the influence of genetics, prenatal and nutritional factors in free range hens still need to be investigated. This review provides information about practices that have been tested or still need to be explored and this information can be used by stakeholders and researchers to help them evaluate the applicability of these solutions for welfare improvement.

Published
2022

18. The multi-actor PPILOW European project: a participative approach to co-build innovations for welfare improvement in organic pig and poultry farms

Author

Micheloni, Cristina, Jamart, An, Re, Martina, van Vooren, Laura, Niemi, Jarkko, Tuyttens, Frank A.M., Rodenburg, Tb (bas), Roinsard, Antoine, Steenfeldt, Sanna, Thobe, Petra, Carelli, Riccardo, Sciarretta, Marlene, Bonnefous, Claire, Bodin, Pauline, Rosati, Andrea, van den Brand, Henry, Rocchi, Lucia, Reverchon, Maxime, Decruyenaere, Virginie, Wavreille, José, Grete, Brunsgaard, Depraetere, Stefaan, Grenier, Katia, Vanggaard, Jan, Ponzio, Raffaella, Guesdon, Vanessa, Leruste, Hélène, Walley, Keith, Cozma, Vasile, Le Bihan-Duval, Elisabeth, Engberg, Ricarda M., Réhault-Godbert, Sophie, Baldinger, Lisa, Thys, Mirjan, Prunier, Armelle, Merlot, Elodie, Tallet, Céline, Montagne, Lucile, Canario, Laurianne, Delanoue, Elsa, Courboulay, Valérie, Hercule, Jonathan, Leterrier, Christine, Latchoumia, Joselle, Laura, Warin, Collin, Anne, Associazione Italiana per l'Agricoltura Biologica (AIAB), BioForum Vlaanderen, Scuela Santa Anna (SSSA), Scuola Universitaria Superiore Sant'Anna [Pisa] (SSSUP), Natural Resources Institute Finland (LUKE), Research Institute for Agricultural, Fisheries and Food (ILVO), Utrecht University [Utrecht], Institut Technique de l'Agriculture Biologique (ITAB), Aarhus University [Aarhus], Thuenen Institute of Farm Economics, European Association for Animal Production (EAAP), Biologie des Oiseaux et Aviculture (BOA), Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Les instituts techniques agricoles (Acta), Wageningen University and Research [Wageningen] (WUR), Università degli Studi di Perugia = University of Perugia (UNIPG), Syndicat des Sélectionneurs Avicoles et Aquacoles Français (SYSAAF), Centre Wallon de Recherches Agronomiques (CRA-W), Fermentation Experts, Circular Organics, Équipe Micro et nanosystèmes HyperFréquences Fluidiques (LAAS-MH2F), Laboratoire d'analyse et d'architecture des systèmes (LAAS), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT), Vanggaard Staldmontage, Slow Food Biodiversity, JUNIA (JUNIA), Université catholique de Lille (UCL), Harper Adams University, USAMV Cluj-Napoca, Thuenen-Institute of Organic Farming, Physiologie, Environnement et Génétique pour l'Animal et les Systèmes d'Elevage [Rennes] (PEGASE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-INSTITUT AGRO Agrocampus Ouest, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-École nationale supérieure agronomique de Toulouse (ENSAT), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut de l'élevage (IDELE), Institut du Porc (IFIP), ITAVI, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), INRAE Transfert, The PPILOW project has received funding from the European Union’s Horizon 2020 Research and Innovation Programme under grant agreement N°816172., IFOAM, PPILOW, and European Project: 816172,H2020-EU.3.2.1.1. - Increasing production efficiency and coping with climate change, while ensuring sustainability and resilience ,PPILOW (2019)

Subjects
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences, [SDV.GEN]Life Sciences [q-bio]/Genetics, [SCCO]Cognitive science, [SDV.BA]Life Sciences [q-bio]/Animal biology, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, [SDV.BDD]Life Sciences [q-bio]/Development Biology, ComputingMilieux_MISCELLANEOUS, [SHS]Humanities and Social Sciences
Abstract

International audience

Published
2021

19. PPILOW 1st technical report. Part B

Author

Collin, Anne, Niemi, Jarkko, Leterrier, Christine, Walley, Keith, Thobe, Petra, Micheloni, Cristina, Re, Martina, Tuyttens, Frank A.M., Warin, Laura, Rocchi, Lucia, Bas Rodenburg, T., Prunier, Armelle, Roinsard, Antoine, Baldinger, Lisa, Réhault-Godbert, Sophie, Steenfeldt, Sanna, Le Bihan-Duval, Elisabeth, van den Brand, Henry, Engberg, Ricarda M., Canario, Laurianne, Castellini, Cesare, Hercule, Jonathan, Carelli, Riccardo, Sciarretta, Marlene, Lagendijk, Emmanuelle, Vermue, Anthony, Biologie des Oiseaux et Aviculture (BOA), Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Natural Resources Institute Finland (LUKE), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Harper Adams University, Thuenen Institute of Farm Economics, Associazione Italiana per l'Agricoltura Biologica (AIAB), Scuela Santa Anna (SSSA), Scuola Universitaria Superiore Sant'Anna [Pisa] (SSSUP), Research Institute for Agricultural, Fisheries and Food (ILVO), ITAVI, Università degli Studi di Perugia = University of Perugia (UNIPG), Utrecht University [Utrecht], Physiologie, Environnement et Génétique pour l'Animal et les Systèmes d'Elevage [Rennes] (PEGASE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Rennes Angers, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut Technique de l'Agriculture Biologique (ITAB), Thuenen-Institute of Organic Farming, Aarhus University [Aarhus], Wageningen University and Research [Wageningen] (WUR), Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-École nationale supérieure agronomique de Toulouse (ENSAT), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), European Association for Animal Production (EAAP), INRAE Transfert, The project PPILOW has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement N°816172, INRAE, PPILOW, and European Project: 816172,H2020-EU.3.2.1.1. - Increasing production efficiency and coping with climate change, while ensuring sustainability and resilience ,PPILOW (2019)

Subjects
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences, [SCCO]Cognitive science, [SDV.GEN]Life Sciences [q-bio]/Genetics, [SDV.BA]Life Sciences [q-bio]/Animal biology, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, [SDV.BDD]Life Sciences [q-bio]/Development Biology, [SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM], [SHS]Humanities and Social Sciences
Published
2021

20. The eggshell microbiome

Author

Réhault-Godbert, Sophie, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), LE STUDIUM | Institute for advanced studies - Loire Valley, and REHAULT-GODBERT, Sophie

Subjects
[SDV] Life Sciences [q-bio], animal structures, [SDV]Life Sciences [q-bio], embryonic structures
Abstract

International audience; Avian eggs possess very efficient and orchestrated systems to protect the embryo during incubation, until hatch. Although the internal components of the egg are assumed to be sterile, the surface of the eggshell is covered by microbes (essentially bacteria) that may contribute to prevent eggshell colonization by pathogenic bacteria, through direct inhibition and/or competitive exclusion. The composition of the eggshell microbiome is a heritage from both maternal microbiota (caeca/faeces) where the egg meets caecal secretions in the cloacal segment during oviposition, and from the nesting environment (contaminated litter/feathers and air environment including dust). At laying time, the egg surface is still moisturized, but will progressively dehydrate during incubation. The surface characteristics of the egg, the loss of the moisture layer and the presence of antimicrobial molecules composing the cuticle are likely to dictate the bacterial communities that will survive on the surface of the eggshell. Although the literature on the composition of the eggshell microbiome of eggs originating from current commercial chicken hens is quite sporadic, this talk aims to provide an overview of the bacterial communities that colonize the chicken eggshell surface, and will discuss the role of the eggshell microbiota as the first barrier against pathogenic bacteria.

Published
2021

21. Innate immunity in a biomineralized context: trade-offs or synergies?

Author

Hincke, Maxwell, Réhault-Godbert, Sophie, University of Ottawa [Ottawa], Biologie des Oiseaux et Aviculture (BOA), Université de Tours-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), LE STUDIUM | Institute for advanced studies - Loire Valley, Maxwell Hincke, Sophie Réhault-Godbert, and Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
[SDV]Life Sciences [q-bio], bacteria, biochemical phenomena, metabolism, and nutrition
Abstract

International audience; Biomineralized structures can function as a barrier to the external environment, and as such are conceptually entwined with innate immune processes. Disentangling immune and biomineralization mechanisms represents a significant challenge for researchers trying to understand how organisms could integrate biomineral formation and plasticity with maintenance of critical innate immune protection. In fact, there is increasing evidence that immune proteins can serve genuine dual-functional roles, both in regulation of biomineralization, as well as resisting pathogens. This awareness is growing in models as diverse as the dual-functionning haemocytes of marine bivalves, and in mineralization / demineralization of the avian eggshell. Moreover, reef corals, in which calcification is coupled to the photosynthetic activity of their mutualistic symbiots, must adapt their innate immune system to achieve this tolerance. Cnidarian immune-related processes in response to abiotic stresses are increasingly implicated in loss of symbiosis and coral bleaching.This conference aims to bring together scientists working with diverse models of biomineralization, for an exchange of perspectives on the innate immune function of the calcified barrier and the dual role played by specific genes / proteins in these two critical functions.

Published
2021

22. How Egg Storage Duration Prior to Incubation Impairs Egg Quality and Chicken Embryonic Development: Contribution of Imaging Technologies.

Author

Adriaensen, Hans, Parasote, Vanille, Castilla, Ines, Bernardet, Nelly, Halgrain, Maeva, Lecompte, François, and Réhault-Godbert, Sophie

Subjects
EMBRYOLOGY, EGG quality, EGG storage, MAGNETIC resonance imaging, EGGS
Abstract

Storing fertilised eggs prior to incubation is a frequent practice in commercial hatcheries to coordinate activities and synchronise hatchings. However, the conditions used to store eggs can have major impacts on egg quality and the subsequent viability of chicken embryos. While storage temperatures of 16–18°C are classically used in hatcheries, the duration of storage varies from three to more than 10 days. We explored the effect of storage duration (zero, three or 10 days; D0, D3 and D10, respectively) at 16°C, 80% relative humidity (RH) on egg quality (Broiler, Ross 308), using computed tomography (CT) and classical measurements (egg weight, eggshell strength, egg white pH, Haugh units, yolk index and colour). The results revealed that a storage duration of up to 10 days negatively affected some egg quality traits (yolk index and volume, air chamber volume and egg white pH). Eggs stored for three or 10 days were further incubated for 11, 13 or 15 days (37.8°C, 55% RH). Eggs were analysed by magnetic resonance imaging (MRI) and CT to assess the development of the embryo and internal egg changes occurring during incubation. First, data showed that the fertility and sex ratio of eggs were not affected by storage duration. However, the mortality of viable eggs was increased in the D10 group compared to the D3 group. Results of non-invasive imaging technologies revealed that the storage of eggs for 10 days impaired embryo growth as early as 11 days of incubation (decrease in brain and embryo volumes). Collectively, these data provide new evidence that the duration of egg storage negatively affects embryonic growth. They further corroborate that this parameter is likely to be crucial to synchronising embryonic stages and maybe reducing the hatching window, hence limiting the time spent by newborn chicks in hatchers. In addition, our results highlight that CT and MRI imaging technologies are useful non-invasive tools to evaluate egg quality prior to incubation and the impact of storage (or incubation) practices on developmental growth of the embryo. [ABSTRACT FROM AUTHOR]

Published
2022
Full Text
View/download PDF

23. Concomitant Morphological Modifications of the Avian Eggshell, Eggshell Membranes and the Chorioallantoic Membrane During Embryonic Development.

Author

Halgrain, Maeva, Georgeault, Sonia, Bernardet, Nelly, Hincke, Maxwell T., and Réhault-Godbert, Sophie

Subjects
CHORIOALLANTOIS, EMBRYOLOGY, EGGSHELLS, CALCIUM ions, SCANNING electron microscopy
Abstract

The chicken eggshell (ES) consists of 95% calcium carbonate and 3.5% organic matter, and represents the first physical barrier to protect the developing embryo, while preventing water loss. During the second half of development, calcium ions from the inner ES are progressively solubilized to support mineralization of the embryonic skeleton. This process is mediated by the chorioallantoic membrane (CAM), which is an extraembryonic structure that adheres to the eggshell membranes (ESM) lining the inner ES. The CAM surrounds the embryo and all egg contents by day 11 of incubation (Embryonic Incubation Day 11, EID11) and is fully differentiated and functionally active by day 15 of incubation (Embryonic Incubation Day 15, EID15). In this study, we explored the simultaneous morphological modifications in the ES, ESM and the CAM at EID11 and EID15 by scanning electron microscopy. We observed that the tips of the mammillary knobs of the ES remain tightly attached to the ESM fibers, while their bases become progressively eroded and then detached from the bulk ES. Concomitantly, the CAM undergoes major structural changes that include the progressive differentiation of villous cells whose villi extend to reach the ESM and the ES. These structural data are discussed with respect to the importance of ES decalcification in providing the calcium necessary for mineralization of embryo's skeleton. In parallel, eggshell decalcification and weakening during incubation is likely to impair the ability of the ES to protect the embryo. It is assumed that the CAM could counteract this apparent weakening as an additional layer of physical, cellular and molecular barriers against environmental pressures, including pathogens, dehydration and shocks. However, such hypothesis needs to be further investigated. [ABSTRACT FROM AUTHOR]

Published
2022
Full Text
View/download PDF

24. Storage Temperature or Thermal Treatments During Long Egg Storage Duration Influences Hatching Performance and Chick Quality.

Author

Guinebretière, Maryse, Puterflam, Julie, Keïta, Alassane, Réhault-Godbert, Sophie, Thomas, Rodolphe, Chartrin, Pascal, Cailleau-Audouin, Estelle, Coudert, Edouard, and Collin, Anne

Subjects
EGG storage, CHICKS, EMBRYOLOGY, EGGS
Abstract

This study was designed to improve the hatching performance, chick robustness and poultry health in the event of long-term egg storage and suboptimal age of the reproductive flock. A total of 9,600 eggs from one young breeder flock (28 weeks of age, batch B) and 9,600 eggs from an older breeder flock (59 weeks of age, batch E) were used (ROSS 308). Each batch was separated into three sub-groups and stored for 14 days. The first sub-group of eggs (Cool, group C) was stored at 11.6°C. The second sub-group of eggs (Warm, group W) was stored at 18.3°C with two pre-incubation on days 6 and 10 of the storage period. The final sub-group of eggs (Control, group Ct) was stored at 18.3°C throughout the storage period. Eggs were similarly incubated and hatched birds were raised on the same experimental farm. In both batches, embryonic development was significantly more advanced in W eggs than in C and Ct eggs (p < 0.01). In both batches, C and W treatments decreased early embryonic mortality by more than 10% compared with Ct, decreased the proportion of late-hatched chicks and improved the percentage of first grade chicks: in batch E, 42% of Ct eggs were first grade chicks vs. 57% in group W and 59% in group C. Benefits were even higher in batch B, where only 60% of Ct eggs gave first grade chicks vs. 83% in others groups. The hatching rate was thus higher in groups C and W regardless of flock age: for batch B eggs, 85% hatched in W and 84% in C vs. 62% in Ct, while for batch E eggs, 59% hatched in W and 61% in C vs. 45% in Ct. Day-old Ct chicks from batch E were heavier than W and C ones, and heavier than W chicks from batch B (p < 0.05). Long-term parameters on farm were not significantly different between groups. Thermal treatments during the storage of eggs from both young and old breeder flocks counterbalance the negative effects of prolonged egg storage on hatching rate, without altering chicken performance during rearing. [ABSTRACT FROM AUTHOR]

Published
2022
Full Text
View/download PDF

25. Impact of variation in energy stores on reproduction and egg characteristics of meat-type chickens

Author

Metayer-Coustard, Sonia, Franzoni, Alessandro, Réhault-Godbert, Sophie, Collin, Anne, Travel, Angélique, RAYNAUD, Emilie, Baumard, Yannick, Delaveau, Joël, Rat, Christophe, Audouin, Estelle, Godet, Estelle, Brionne, Aurélien, Berri, Cécile, Duval, Elisabeth, Biologie des Oiseaux et Aviculture (BOA), Institut National de la Recherche Agronomique (INRA)-Université de Tours, ITAVI, Pôle d'Expérimentation Avicole de Tours (UE PEAT), Institut National de la Recherche Agronomique (INRA), and Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)

Subjects
[SDV.GEN]Life Sciences [q-bio]/Genetics, [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2019

26. Metabolomic analysis of nutrient sources in the embryonic egg of two divergent lines for meat ultimate pH

Author

Petit, Angélique, Réhault-Godbert, Sophie, Nadal-Desbarats, Lydie, Audouin, Estelle, Chartrin, Pascal, RAYNAUD, Emilie, Bernardet, Nelly, Chessé, Magali, Duval, Elisabeth, Metayer-Coustard, Sonia, Biologie des Oiseaux et Aviculture (BOA), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Université de Tours (UT), Institut National de la Recherche Agronomique (INRA)-Université de Tours, and Université de Tours

Subjects
[SDV.GEN]Life Sciences [q-bio]/Genetics, [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2019

27. From eggshell decalcification to skeleton mineralization of chicken embryos during incubation

Author

Crépeau, Marianne, Bernardet, Nelly, Meme, Nathalie, Narcy, Agnès, Hincke, Maxwell, Réhault-Godbert, Sophie, Biologie des Oiseaux et Aviculture (BOA), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), University of Ottawa [Ottawa], and Institut National de la Recherche Agronomique (INRA)-Université de Tours

Subjects
[SDV.GEN]Life Sciences [q-bio]/Genetics, [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2019

28. Evolution of innate immunity components during incubation of the avian egg

Author

Réhault-Godbert, Sophie, Da Silva, Mylène, Guyot, Nicolas, Gautron, Joël, GUABIRABA-BRITO, Rodrigo, Hincke, Maxwell, Biologie des Oiseaux et Aviculture (BOA), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Infectiologie et Santé Publique (UMR ISP), University of Ottawa [Ottawa], and Institut National de la Recherche Agronomique (INRA)-Université de Tours

Subjects
[SDV.GEN]Life Sciences [q-bio]/Genetics, [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2019

29. Novel egg components and human health

Author

Réhault-Godbert, Sophie, Biologie des Oiseaux et Aviculture (BOA), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), and Institut National de la Recherche Agronomique (INRA)-Université de Tours

Subjects
[SDV.GEN]Life Sciences [q-bio]/Genetics, [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, egg, human health, neutraceuticals, digestion, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2019

30. Gga-AvBD11, the archetype of a new structural family of defensins

Author

Meudal, H., Guyot, Nicolas, Loth, K., Jousset, G., Trapp, S., Iochmann, S., Silvestre, A., Labas, Valérie, Reverdiau, P., Hervé, V., Aucagne, V., Delmas, A.F., Réhault-Godbert, Sophie, Landon, C., Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Biologie des Oiseaux et Aviculture (BOA), Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Centre d’Etude des Pathologies Respiratoires (CEPR), UMR 1100 (CEPR), Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), Neurobiologie et diversité cellulaire (NDC), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM), Frapart, Isabelle, Université de Tours-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National de la Recherche Agronomique (INRA)-Université de Tours, and Université de Tours-Institut National de la Santé et de la Recherche Médicale (INSERM)

Subjects
[SDV]Life Sciences [q-bio], [CHIM] Chemical Sciences, [CHIM]Chemical Sciences, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2019

31. A new structural family for antimicrobial defensins

Author

Meudal, H., Loth, K., Jousset, G., Aucagne, V., Guyot, Nicolas, Réhault-Godbert, Sophie, Delmas, A.F., Landon, C., Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Biologie des Oiseaux et Aviculture (BOA), Université de Tours-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Frapart, Isabelle, and Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
[CHIM] Chemical Sciences, [CHIM]Chemical Sciences, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2019

32. CHAPTER 14. Bioactive Minor Egg Components

Author

Gautron, Joël, Guyot, Nicolas, Brionne, Aurélien, Réhault-Godbert, Sophie, Biologie des Oiseaux et Aviculture (BOA), Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Wu J., and Université de Tours-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
[SDV.GEN]Life Sciences [q-bio]/Genetics, lysosyme, nutriment, [SDV]Life Sciences [q-bio], coquille d'oeuf, santé humaine, ovotransferrine, [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, ovalbumine, aliment santé pour l'homme, protéine, [INFO]Computer Science [cs], composition des aliments, ComputingMilieux_MISCELLANEOUS, oeuf de poule
Abstract

International audience

Published
2019

33. Proteomic study of inner and outer hen egg vitelline membranes: insights into the biological functions of vitelline membrane layers

Author

Brégeon, Mégane, Bernay, B., Labas, Valérie, Chessé, Magali, Zatylny-Gaudin, Céline, Réhault-Godbert, Sophie, Guyot, Nicolas, and REHAULT-GODBERT, Sophie

Subjects
[SDV] Life Sciences [q-bio], proteomics, protein identification, protein function, hen egg, outer/inner vitelline membrane
Abstract

The hen egg vitelline membrane (VM) is a proteinaceous membrane separating the egg white from the yolk. The VM is involved in fertilization, in early stages of embryogenesis but also in protection of the embryo. It consists of the inner layer (IVM) and the outer layer (OVM), that are in contact with the yolk and the egg white, respectively. These two layers are synthesized by distinct tissues namely the ovary/liver for IVM and the oviduct for OVM. About 140 different proteins were identified in the VM to date; however, the distribution of most of these proteins between IVM and OVM remains to be elucidated. The VM contains several ZP proteins that are likely to be involved in sperm-oocyte interactions during fertilization. VM is also crucial for embryonic development as it provides a substratum for embryonic and yolk sac expansion in early incubation. Moreover, VM is a physical barrier protecting the embryo from the egg white alkalinity and from potential microbial contaminations, and the high amount of antimicrobials in VM (lysozyme, AvBD11...) also contributes to the antimicrobial defense of the developing embryo. Thus, VM plays a key role, not only in fertilization, but also during incubation. The present study aims at determining the protein composition of IVM and OVM to identify the intrinsic putative functions of each VM layer. IVM and OVM were manually separated from the VM of freshly-laid eggs and proteins from these two layers were independently solubilized, and analyzed by SDS-PAGE and GeLC-MS/MS. More than 550 proteins were identified with two peptides in the whole VM. Around 440 and 380 proteins were identified in IVM and OVM, respectively. Preliminary data resulting from the functional annotation of proteins (gene ontology/bibliography) revealed the presence of many proteins putatively associated with cell adhesion and migration, in addition to proteins involved in fertilization and antimicrobial defense. Altogether, the data generated from this study will give new insights into the structure and composition of the chicken egg VM and will provide an integrative overview of the respective physiological functions of IVM and OVM. Next, it will be interesting to further study how storage conditions prior to incubation may alter the structural/molecular integrity of IVM and OVM, and consequently negatively or positively impact the revival of embryonic development during incubation.

Published
2019

34. Three‐dimensional structures of avian beta‐microseminoproteins: insight from the chicken egg‐specific beta‐microseminoprotein 3 paralog.

Author

Coste, Franck, Moreau, Thierry, Labas, Valérie, Chessé, Magali, Bregeon, Mégane, Meudal, Hervé, Loth, Karine, Castaing, Bertrand, Guyot, Nicolas, Réhault‐Godbert, Sophie, and Wlodawer, Alex

Subjects
BASIC proteins, HENS, BIRD conservation, EGG whites, CHICKENS, BIRD eggs, THIOUREA
Abstract

Beta‐microseminoproteins (MSMBs) are small disulfide‐rich proteins that are conserved among vertebrates. These proteins exhibit diverse biological activities and were mainly reported to play a role in male fertility, immunity, and embryogenesis. In this work, we focused on the chicken MSMB3 protein that was previously depicted as an egg antibacterial protein. We report that MSMB3 protein is exclusively expressed in the reproductive tissues of laying hens (in contrast to chicken MSMB1 and MSMB2 paralogs), to be incorporated in the egg white during the process of egg formation. We also showed that chicken MSMB3 possesses highly conserved orthologs in bird species, including Neognathae and Palaeognathae. Chicken MSMB3 was purified from egg white using heparin affinity chromatography and was analyzed by top‐down and bottom‐up proteomics. Several proteoforms could be characterized, and a homodimer was further evidenced by NMR spectroscopy. The X‐ray structure of chicken MSMB3 was solved for the first time, revealing that this protein adopts a novel dimeric arrangement. The highly cationic MSMB3 protein exhibits a distinct electrostatic distribution compared with chicken MSMB1 and MSMB2 structural models, and with published mammalian MSMB structures. The specific incorporation of MSMB3 paralog in the egg, and its phylogenetic conservation in birds together with its peculiar homodimer arrangement and physicochemical properties, suggests that the MSMB3 protein has evolved to play a critical role during the embryonic development of avian species. These new data are likely to stimulate research to elucidate the structure/function relationships of MSMB paralogs and orthologs in the animal kingdom. [ABSTRACT FROM AUTHOR]

Published
2021
Full Text
View/download PDF

35. Transcriptomic profiling of proteases and antiproteases in the liver of sexually mature hens in relation to vitellogenesis

Author

Bourin Marie, Gautron Joël, Berges Magali, Hennequet-Antier Christelle, Cabau Cédric, Nys Yves, and Réhault-Godbert Sophie

Subjects
Proteases, Antiproteases, Liver, Sex steroids, Metabolism, Transcriptomics, Chicken egg, Vitellogenesis, Biotechnology, TP248.13-248.65, Genetics, QH426-470
Abstract

Abstract Background Most egg yolk precursors are synthesized by the liver, secreted into the blood and transferred into oocytes, to provide nutrients and bioactive molecules for the avian embryo. Three hundred and sixteen distinct proteins have been identified in egg yolk. These include 37 proteases and antiproteases, which are likely to play a role in the formation of the yolk (vitellogenesis), as regulators of protein metabolism. We used a transcriptomic approach to define the protease and antiprotease genes specifically expressed in the hen liver in relation to vitellogenesis by comparing sexually mature and pre-laying chickens showing different steroid milieu. Results Using a 20 K chicken oligoarray, a total of 582 genes were shown to be over-expressed in the liver of sexually mature hens (1.2 to 67 fold-differences). Eight of the top ten over-expressed genes are known components of the egg yolk or perivitelline membrane. This list of 582 genes contains 12 proteases and 3 antiproteases. We found that “uncharacterized protein LOC419301/similar to porin” (GeneID:419301), an antiprotease and “cathepsin E-A-like/similar to nothepsin” (GeneID:417848), a protease, were the only over-expressed candidates (21-fold and 35-fold difference, respectively) that are present in the egg yolk. Additionally, we showed the 4-fold over-expression of “ovochymase-2/similar to oviductin” (GeneID:769290), a vitelline membrane-specific protease. Conclusions Our approach revealed that three proteases and antiproteases are likely to participate in the formation of the yolk. The role of the other 12 proteases and antiproteases which are over-expressed in our model remains unclear. At least 1/3 of proteases and antiproteases identified in egg yolk and vitelline membrane proteomes are expressed similarly in the liver regardless of the maturity of hens, and have been initially identified as regulators of haemostasis and inflammatory events. The lack of effect of sex steroids on these genes expressed in the liver but the products of which are found in the yolk suggests that these may be passively incorporated into the yolk rather than actively produced for that purpose. These results raise the question of the biological significance of egg yolk proteases and antiproteases, and more generally of all minor proteins that have been identified in egg yolk.

Published
2012
Full Text
View/download PDF

36. Gene expression profiling to identify eggshell proteins involved in physical defense of the chicken egg

Author

Sibut Vonick, Cabau Cédric, Hennequet-Antier Christelle, Réhault-Godbert Sophie, Jonchère Vincent, Cogburn Larry A, Nys Yves, and Gautron Joel

Subjects
Biotechnology, TP248.13-248.65, Genetics, QH426-470
Abstract

Abstract Background As uricoletic animals, chickens produce cleidoic eggs, which are self-contained bacteria-resistant biological packages for extra-uterine development of the chick embryo. The eggshell constitutes a natural physical barrier against bacterial penetration if it forms correctly and remains intact. The eggshell's remarkable mechanical properties are due to interactions among mineral components and the organic matrix proteins. The purpose of our study was to identify novel eggshell proteins by examining the transcriptome of the uterus during calcification of the eggshell. An extensive bioinformatic analysis on genes over-expressed in the uterus allowed us to identify novel eggshell proteins that contribute to the egg's natural defenses. Results Our 14 K Del-Mar Chicken Integrated Systems microarray was used for transcriptional profiling in the hen's uterus during eggshell deposition. A total of 605 transcripts were over-expressed in the uterus compared with the magnum or white isthmus across a wide range of abundance (1.1- to 79.4-fold difference). The 605 highly-expressed uterine transcripts correspond to 469 unique genes, which encode 437 different proteins. Gene Ontology (GO) analysis was used for interpretation of protein function. The most over-represented GO terms are related to genes encoding ion transport proteins, which provide eggshell mineral precursors. Signal peptide sequence was found for 54 putative proteins secreted by the uterus during eggshell formation. Many functional proteins are involved in calcium binding or biomineralization--prerequisites for interacting with the mineral phase during eggshell fabrication. While another large group of proteins could be involved in proper folding of the eggshell matrix. Many secreted uterine proteins possess antibacterial properties, which would protect the egg against microbial invasion. A final group includes proteases and protease inhibitors that regulate protein activity in the acellular uterine fluid where eggshell formation takes place. Conclusions Our original study provides the first detailed description of the chicken uterus transcriptome during formation of the eggshell. We have discovered a cache of about 600 functional genes and identified a large number of encoded proteins secreted into uterine fluid for fabrication of the eggshell and chemical protection of the egg. Some of these uterine genes could prove useful as biological markers for genetic improvement of phenotypic traits (i.e., egg and eggshell quality).

Published
2010
Full Text
View/download PDF

37. Exploring the role of the chicken amniotic fluid during embryonic development

Author

Da Silva, Mylène, Brionne, Aurélien, Labas, Valérie, Combes Soia, Lucie, Mills, Maryse, Chessé, Magali, Nys, Yves, Réhault-Godbert, Sophie, Unité de Recherches Avicoles (URA), Institut National de la Recherche Agronomique (INRA), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Plate-forme d'Analyse Intégrative des Biomolécules, World's Poultry Science Association (WPSA). INT., ProdInra, Migration, and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, [SDV.BDLR] Life Sciences [q-bio]/Reproductive Biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2016

38. The chicken egg: a multifaced and sophisticated assembly of protective systems

Author

Réhault-Godbert, Sophie, Unité de Recherches Avicoles (URA), and Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV.GEN]Life Sciences [q-bio]/Genetics, [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2016

40. Protein digestion re-visited by proteomics

Author

Duclos, Michel Jacques, Recoules, Emilie, Réhault-Godbert, Sophie, Lessire, Michel, Narcy, Agnès, Gabriel, Irène, Recherches Avicoles (SRA), Institut National de la Recherche Agronomique (INRA), Unité de Recherches Avicoles (URA), and ProdInra, Archive Ouverte

Subjects
volaille, nutrition, [SDV.BA] Life Sciences [q-bio]/Animal biology, [SDV.BA]Life Sciences [q-bio]/Animal biology, protéomique
Abstract

absent

Published
2015

41. Identification of protein fractions in digesta of broilers fed with four different protein sources

Author

Recoules, Emilie, Sabboh-Jourdan, Houda, Narcy, Agnès, LESSIRE, Michel, Harichaux, Grégoire, Duclos, Michel Jacques, Réhault-Godbert, Sophie, Unité de Recherches Avicoles (URA), Institut National de la Recherche Agronomique (INRA), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), ProdInra, Migration, and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV] Life Sciences [q-bio], volaille, digestion, protéomique, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2015

42. Antibacterial activity of egg albumen during incubation

Author

Guyot, Nicolas, Slugocki, Cindy, Helloin, Emmanuelle, Gautron, Joël, Nys, Yves, Réhault-Godbert, Sophie, Unité de Recherches Avicoles (URA), Institut National de la Recherche Agronomique (INRA), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Région, World's Poultry Science Association (WPSA). INT., and Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, embryonic structures
Abstract

National audience; The aim of the present work was to analyse the change of egg white antibacterial activities during egg incubation. Embryonic (E) and unfertilized (U) eggs were incubated for several periods of time, from 0 to 12 days, and the resulting egg whites were analyzed for their pH, protein content and for their antibacterial properties against 5 pathogenic strains. The changes in egg white pH values differed between U and E eggs. Egg white concentrations remained stable during incubation for U eggs but significantly increased for E eggs. At constant protein concentration and for an dentical duration of incubation, egg white activities against Listeria monocytogenes et Streptococcus uberis were similar between E and U groups. A progressive decrease in these activities was however observed depending on the incubation time, regardless of the egg group (U or E). A discrete change in the profile of egg white proteins is also observed during incubation but no difference was noticeable between both groups (E versus U). To conclude, the antibacterial activity of egg white proteins progressively decreased during egg incubation. This observation could result from the alteration of specific antimicrobial proteins. Taken together, these data support that the egg white antibacterial defence is altered during egg incubation, suggesting that new compensatory protection systems might be set up during embryonic development to overcome this apparent weakening.

Published
2015

43. Egg ovalbumins revisited: investigating the specificities of ovalbumin, ovalbumin-related protein X and ovalbumin-related protein Y

Author

Réhault-Godbert, Sophie, Da Silva, Mylène, Beauclercq, Stéphane, Harichaux, Grégoire, Labas, Valérie, Mouton, Grégoire, Guyot, Nicolas, Gautron, Joël, Nys, Yves, ProdInra, Migration, Unité de Recherches Avicoles (URA), Institut National de la Recherche Agronomique (INRA), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], egg, [INFO] Computer Science [cs], protein, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2015

44. Investigating the role of amniotic and chorioallantoic fluids in the protection of the chicken embryo

Author

Da Silva, Mylène, Nys, Yves, Réhault-Godbert, Sophie, ProdInra, Migration, Unité de Recherches Avicoles (URA), Institut National de la Recherche Agronomique (INRA), and Région Centre Val de Loire

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], chicken, allantoic fluid, embryo, amniotic fluid, [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2015

45. Protein digestion in broiler: what are the specificities induced by the protein source in the diet

Author

Recoules, Emilie, Sabboh-Jourdan, Houda, Narcy, Agnès, LESSIRE, Michel, Harichaux, Grégoire, Duclos, Michel Jacques, Réhault-Godbert, Sophie, ProdInra, Migration, Unité de Recherches Avicoles (URA), Institut National de la Recherche Agronomique (INRA), and European Federation of Animal Science (EAAP). INT.

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], proteines, digestion, électrophorèse, [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2015

47. Egg white antimicrobial proteins: identification, characterization and regulation of antimicrobial activities

Author

Nys, Yves, Réhault-Godbert, Sophie, Guyot, Nicolas, Gautron, Joël, Unité de Recherches Avicoles (URA), Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2014

48. Insights into the structure-function relationship of egg defensins

Author

Guyot, Nicolas, Hervé, Virginie, Meudal, Hervé, Madinier, Jean-Baptiste, Labas, Valérie, Berges, Magali, Gautron, Joël, Réhault-Godbert, Sophie, Delmas, Agnès F., Landon, Céline, Nys, Yves, Unité de Recherches Avicoles (URA), Institut National de la Recherche Agronomique (INRA), Université Francois Rabelais [Tours], Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), World's Poultry Science Association (WPSA). INT., Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2014

49. Ovalbumin related protein X is a heparin-binding glycoprotein exhibiting antimicrobial activities

Author

Réhault-Godbert, Sophie, Beauclercq, Stéphane, Chessé, Magali, Gautron, Joël, Coste, F., Guyot, Nicolas, Nys, Yves, Unité de Recherches Avicoles (URA), Institut National de la Recherche Agronomique (INRA), and Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2014

50. Gallin is a chicken egg beta-defensin with a narrow antibacterial spectrum

Author

Hervé, Virginie, Guyot, Nicolas, Meudal, H., Labas, Valérie, Réhault-Godbert, Sophie, Gautron, Joël, Chessé, Magali, Delmas, A.F., Nys, Yves, Landon, C., ProdInra, Migration, Unité de Recherches Avicoles (URA), Institut National de la Recherche Agronomique (INRA), Université Francois Rabelais [Tours], Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Plate-forme d'Analyse Intégrative des Biomolécules, French National Research Agency (OVO-mining, ANR-09-BLAN-0136), Société Française des Peptides Antimicrobiens (SFPA). FRA. Laboratoire de Biotechnologie et Chimie Marines (LBCM). Université de Bretagne Sud (Lorient Vannes) (UBS)., and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], [SDV.OT] Life Sciences [q-bio]/Other [q-bio.OT], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2014

Catalog

Books, media, physical & digital resources
See catalog results