1. Characterisation of IL-21 and IL-21Rα in grass carp: IL-21-producing cells are upregulated during Flavobacterium columnare infection
- Author
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Jiawen Xu, Junya Wang, Qingyu Min, Wei Wang, Yuting Qin, Lina Lei, Qian Gao, and Jun Zou
- Subjects
IL-21 ,IL-21Rα ,Gene expression ,IL-21-Producing cell ,Bacterial and viral infection ,Fish ,Aquaculture. Fisheries. Angling ,SH1-691 - Abstract
Interleukin-21 (IL-21) is produced mainly by activated CD4+ T cells and promotes the proliferation, survival and differentiation of immune cells. This study characterised a high affinity binding receptor of IL-21 (IL-21 Rα) from grass carp (Ctenopharyngodon idella, Ci) and analyzed the expression of CiIL-21 and CiIL-21 Rα. It has been shown that they were modulated by PAMPs and during infection with bacterial and viral pathogens. Recombinant CiIL-21 protein produced in bacteria was active in inducing the expression of genes involved in Th responses. A monoclonal CiIL-21 antibody was generated against the rCiIL-21 and showed good reactive specificity with the rCiIL-21 proteins expressed in E. coli cells, and HEK293 T cells and the native protein from PHA-stimulated kidney leukocytes. In addition, the IL-21-producing cells detected by the CiIL-21 monoclonal antibody were found to be increased to in the kidney, spleen and hindgut after infection with Flavobacterium columnare. Our data indicate that IL-21 is involved in the regulation of immune response to bacterial and viral infection.
- Published
- 2024
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