42 results on '"Pozzi, Ernesto"'
Search Results
2. EGFR and KRAS mutational profiling in fresh non-small cell lung cancer (NSCLC) cells
- Author
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Stella, Giulia M., Scabini, Roberta, Inghilleri, Simona, Cemmi, Francesca, Corso, Simona, Pozzi, Ernesto, Morbini, Patrizia, Valentini, Adele, Dore, Roberto, Ferrari, Simona, Luisetti, Maurizio, and Zorzetto, Michele
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- 2013
- Full Text
- View/download PDF
3. Rescue use of beclomethasone and albuterol in a single inhaler for mild asthma
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Papi, Alberto, Canonica, Giorgio W., Maestrelli, Piero, Paggiaro, Pierluigi, Olivieri, Dario, Pozzi, Ernesto, Crimi, Nunzio, Vignola, Antonio M., Morelli, Paolo, Nicolini, Gabriele, and Fabbri, Leonardo M.
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Asthma -- Drug therapy ,Beclomethasone dipropionate -- Dosage and administration ,Albuterol -- Dosage and administration ,Inhalers -- Usage - Abstract
A study to investigate whether the use of a combination of beclomethasone dipropionate and albuterol in a single inhaler would be as effective as the regular use of inhaled beclomethasone, was conducted. The symptom-driven use of inhaled beclomethasone and albuterol in a single inhaler was found to be as effective as regular use of inhaled beclomethasone.
- Published
- 2007
4. Caveolin-1 as a promoter of tumour spreading: when, how, where and why
- Author
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Senetta, Rebecca, Stella, Giulia, Pozzi, Ernesto, Sturli, Niccolo, Massi, Daniela, and Cassoni, Paola
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- 2013
- Full Text
- View/download PDF
5. MET mutations in cancers of unknown primary origin (CUPs)
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Stella, Giulia M., Benvenuti, Silvia, Gramaglia, Daniela, Scarpa, Aldo, Tomezzoli, Anna, Cassoni, Paola, Senetta, Rebecca, Venesio, Tiziana, Pozzi, Ernesto, Bardelli, Alberto, and Comoglio, Paolo M.
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- 2011
- Full Text
- View/download PDF
6. Long-term Outcome after Pulmonary Endarterectomy
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Corsico, Angelo G., DʼArmini, Andrea M., Cerveri, Isa, Klersy, Catherine, Ansaldo, Elena, Niniano, Rosanna, Gatto, Elena, Monterosso, Cristian, Morsolini, Marco, Nicolardi, Salvatore, Tramontin, Corrado, Pozzi, Ernesto, and Viganò, Mario
- Published
- 2008
- Full Text
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7. Expression of Receptor for Advanced Glycation End Products in Sarcoid Granulomas
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Campo, Ilaria, Morbini, Patrizia, Zorzetto, Michele, Tinelli, Carmine, Brunetta, Enrico, Villa, Chiara, Bombieri, Cristina, Cuccia, Mariaclara, Agostini, Carlo, Bozzi, Valeria, Facoetti, Angelica, Ferrarotti, Ilaria, Mazzola, Paola, Scabini, Roberta, Semenzato, Gianpietro, Pignatti, Pier Franco, Pozzi, Ernesto, and Luisetti, Maurizio
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- 2007
- Full Text
- View/download PDF
8. Assessment of Emphysema in COPD*: A Functional and Radiologic Study
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Cerveri, Isa, Dore, Roberto, Corsico, Angelo, Zoia, Maria C., Pellegrino, Riccardo, Brusasco, Vito, and Pozzi, Ernesto
- Published
- 2004
9. Complement Receptor 1 Gene Polymorphisms Are Associated with Idiopathic Pulmonary Fibrosis
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Zorzetto, Michele, Ferrarotti, Ilaria, Trisolini, Rocco, Agli, Luigi Lazzari, Scabini, Roberta, Novo, Monique, De Silvestri, Annalisa, Patelli, Marco, Martinetti, Miryam, Cuccia, MariaClara, Poletti, Venerino, Pozzi, Ernesto, and Luisetti, Maurizio
- Published
- 2003
- Full Text
- View/download PDF
10. Complement Receptor 1 Gene Polymorphisms in Sarcoidosis
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Zorzetto, Michele, Bombieri, Cristina, Ferrarotti, Ilaria, Medaglia, Sara, Agostini, Carlo, Tinelli, Carmine, Malerba, Giovanni, Carrabino, Natalia, Beretta, Anna, Casali, Lucio, Pozzi, Ernesto, Pignatti, Pier Franco, Semenzato, Gianpietro, Cuccia, Maria Clara, and Luisetti, Maurizio
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- 2002
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11. Circulating Adhesion Molecules in Cystic Fibrosis
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DE ROSE, VIRGINIA, OLIVA, ALESSANDRO, MESSORE, BARBARA, GROSSO, BIANCA, MOLLAR, CINZIA, and POZZI, ERNESTO
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- 1998
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12. PHARMACOLOGICAL ANALYSIS OF SIGNAL TRANSDUCTION PATHWAYS REQUIRED FOR MYCOBACTERIUM TUBERCULOSIS -INDUCED IL-8 AND MCP-1 PRODUCTION IN HUMAN PERIPHERAL MONOCYTES
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Fietta, Anna M, Morosini, Monica, Meloni, Federica, Bianco, Alessia Marone, and Pozzi, Ernesto
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- 2002
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13. Release of prostaglandin E2 and leukotriene B4 by alveolar macrophages from patients with sarcoidosis
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De Rose, Virginia, Trentin, Livio, Crivellari, Maria T., Cipriani, Angiolo, Grassi, Giuliana Gialdroni, Pozzi, Ernesto, Folco, Giancarlo, and Semenzato, Gianpietro
- Published
- 1997
14. Successful whole lung lavage in pulmonary alveolar proteinosis secondary to lysinuric protein intolerance: a case report
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Baritussio Aldo, Bolongaro Antonia, Adami Andrea, Stella Giulia M, Rodi Giuseppe, Ceruti Michele, Pozzi Ernesto, and Luisetti Maurizio
- Subjects
Medicine - Abstract
Abstract Background Pulmonary alveolar proteinosis (PAP) is a rare disease characterised by accumulation of lipoproteinaceous material within alveoli, occurring in three clinically distinct forms: congenital, acquired and secondary. Among the latter, lysinuric protein intolerance (LPI) is a rare genetic disorder caused by defective transport of cationic amino acids. Whole Lung Lavage (WLL) is currently the gold standard therapy for severe cases of PAP. Case presentation We describe the case of an Italian boy affected by LPI who, by the age of 10, developed digital clubbing and, by the age of 16, a mild restrictive functional impairment associated with a high-resolution computed tomography (HRCT) pattern consistent with pulmonary alveolar proteinosis. After careful assessment, he underwent WLL. Conclusion Two years after WLL, the patient has no clinical, radiological or functional evidence of pulmonary disease recurrence, thus suggesting that WLL may be helpful in the treatment of PAP secondary to LPI.
- Published
- 2007
- Full Text
- View/download PDF
15. Intercellular Adhesion Molecule-1 Is Upregulated on Peripheral Blood T Lymphocyte Subsets in Dual Asthmatic Responders
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De Rose, Virginia, Rolla, Giovanni, Bucca, Caterina, Ghio, Paolo, Bertoletti, Massimo, Baderna, Paolo, and Pozzi, Ernesto.
- Published
- 1994
16. SP-A binds alpha1-antitrypsin in vitro and reduces the association rate constant for neutrophil elastase
- Author
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Carrabino Natalia, Dalzoppo Daniele, Rognoni Paola, Santos Conceição, Iadarola Paolo, Lupi Anna, Gorrini Marina, Pozzi Ernesto, Baritussio Aldo, and Luisetti Maurizio
- Subjects
Diseases of the respiratory system ,RC705-779 - Abstract
Abstract Background α1-antitrypsin and surfactant protein-A (SP-A) are major lung defense proteins. With the hypothesis that SP-A could bind α1-antitrypsin, we designed a series of in vitro experiments aimed at investigating the nature and consequences of such an interaction. Methods and results At an α1-antitrypsin:SP-A molar ratio of 1:1, the interaction resulted in a calcium-dependent decrease of 84.6% in the association rate constant of α1-antitrypsin for neutrophil elastase. The findings were similar when SP-A was coupled with the Z variant of α1-antitrypsin. The carbohydrate recognition domain of SP-A appeared to be a major determinant of the interaction, by recognizing α1-antitrypsin carbohydrate chains. However, binding of SP-A carbohydrate chains to the α1-antitrypsin amino acid backbone and interaction between carbohydrates of both proteins are also possible. Gel filtration chromatography and turnover per inactivation experiments indicated that one part of SP-A binds several molar parts of α1-antitrypsin. Conclusion We conclude that the binding of SP-A to α1-antitrypsin results in a decrease of the inhibition of neutrophil elastase. This interaction could have potential implications in the physiologic regulation of α1-antitrypsin activity, in the pathogenesis of pulmonary emphysema, and in the defense against infectious agents.
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- 2005
- Full Text
- View/download PDF
17. Non-neuronal cholinergic system in airways and lung cancer susceptibility
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Saracino, Laura, Zorzetto, Michele, Inghilleri, Simona, Pozzi, Ernesto, and Stella, Giulia Maria
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Review Article - Abstract
In the airway tract acetylcholine (ACh) is known to be the mediator of the parasympathetic nervous system. However ACh is also synthesized by a large variety of non-neuronal cells. Strongest expression is documented in neuroendocrine and in epithelial cells (ciliated, basal and secretory elements). Growing evidence suggests that a cell-type specific Ach expression and release do exist and act with local autoparacrine loop in the non-neuronal airway compartment. Here we review the molecular mechanism by which Ach is involved in regulating various aspects of innate mucosal defense, including mucociliary clearance, regulation of macrophage activation as well as in promoting epithelial cells proliferation and conferring susceptibility to lung carcinoma onset. Importantly this non-neuronal cholinergic machinery is differently regulated than the neuronal one and could be specifically therapeutically targeted.
- Published
- 2013
18. Mutational profiling of EGFR and KRAS oncogenes in non-small cell lung cancer (NSCLC) cells obtained through fine needle aspiration cytology (FNAC)
- Author
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Cemmi, Francesca, Stella, Giulia Maria, Zorzetto, Michele, Inghilleri, Simona, Morbini, Patrizia, Dore, Roberto, and Pozzi, Ernesto
- Abstract
Cancer is a genetic disease and this concept has now been widely exploited by both biologists and clinicians to design new targeted therapeutical approaches. Indeed many data have already allowed us to ameliorate not only our knowledge about cancer onset, but also about patient treatment. Correlation between mutations in cancer alleles and drug response is a crucial point to identify drugs or drug combinations that match the genetic profile of individual tumors. On the other hand, experiences derived from receptor tyrosine kinases (RTKs) inhibition have pointed out that targeted treatment is really successful only in a small subset of tumors. The latter are eventually addicted to the genetic alterations responsible for receptors activation and continued expression of their signaling pathways. Therefore, switching off the oncogenic activity by specific inhibitors will trigger an “oncogenic shock” which eventually will lead tumor cell to die. Overall these observations provide a strong rationale for molecular-based diagnosis and cancer patients selection for targeted treatment. Understanding how the mutational status of oncogenic alleles affects sensibility or resistance to drugs represents the most potential strategy in identifying patients for effective personalized therapies. In consideration of the high incidence and the poor prognosis of affected patients, lung carcinoma has been considered and still identify major objective of the targeted therapeutic approach. Experiences derived from this disease, and mainly from non-small cell lung cancer (NSCLC), represent fundamental achievement of translational research. From this perspective, members of the EGFR signal transduction pathway play a key role in lung carcinogenesis and act as genetic markers predictive of response to anti-EGFR therapy. In unselected NSCLCs EGFR activating mutations are rarely present (10%); mutation frequency increases to over 50% in a restricted subset of NSCLC patients: East-Asian, women, non smokers, affected by ADC (mainly BAC variant). Moreover the incidence of EGFR mutations in NSCLCs increases up to 77% among EGFR TKIs responders, while it is 7% in unsensitive cases. This project aims to evaluate in a cohort of NSCLC patients the prevalence of activating mutations in EGFR and KRAS genes by analyzing tumor cells obtained through transthoracic biopsy by FNAC, which actually represents the most appropriate diagnostic tool for peripheral lesions, such as ADKs that actually account for 40% of NSCLC diagnosis. Validation of sequencing technology on tumor samples constituted by only few cells has two relevant implications. First it might allows routinary tumor molecular profiling as powerful integration of conventional histo-pathological diagnosis. Besides the clinical management of NSCLC will take advantages from the characterization of EGFR-KRAS mutational status in order to develop personalized and effective treatment strategies., Bollettino della Società Medico Chirurgica di Pavia, Vol 123, N° 2 (2010)
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- 2010
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19. The combination of sorafenib and everolimus shows antitumor activity in preclinical models of malignant pleural mesothelioma.
- Author
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Pignochino, Ymera, Dell'Aglio, Carmine, Inghilleri, Simona, Zorzetto, Michele, Basiricò, Marco, Capozzi, Federica, Canta, Marta, Piloni, Davide, Cemmi, Francesca, Sangiolo, Dario, Gammaitoni, Loretta, Soster, Marco, Marchiò, Serena, Pozzi, Ernesto, Morbini, Patrizia, Luisetti, Maurizio, Aglietta, Massimo, Grignani, Giovanni, and Stella, Giulia M.
- Subjects
EVEROLIMUS ,ANTINEOPLASTIC agents ,DRUG activation ,CLINICAL trials ,RADIXIN ,IMMUNOPHENOTYPING - Abstract
Background: Malignant Pleural Mesothelioma (MPM) is an aggressive tumor arising from mesothelial cells lining the pleural cavities characterized by resistance to standard therapies. Most of the molecular steps responsible for pleural transformation remain unclear; however, several growth factor signaling cascades are known to be altered during MPM onset and progression. Transducers of these pathways, such as PIK3CA-mTOR-AKT, MAPK, and ezrin/radixin/moesin (ERM) could therefore be exploited as possible targets for pharmacological intervention. This study aimed to identify 'druggable' pathways in MPM and to formulate a targeted approach based on the use of commercially available molecules, such as the multikinase inhibitor sorafenib and the mTOR inhibitor everolimus. Methods: We planned a triple approach based on: i) analysis of immunophenotypes and mutational profiles in a cohort of thoracoscopic MPM samples, ii) in vitro pharmacological assays, ii) in vivo therapeutic approaches on MPM xenografts. No mutations were found in 'hot spot' regions of the mTOR upstream genes (e.g. EGFR, KRAS and PIK3CA). Results: Phosphorylated mTOR and ERM were specifically overexpressed in the analyzed MPM samples. Sorafenib and everolimus combination was effective in mTOR and ERM blockade; exerted synergistic effects on the inhibition of MPM cell proliferation; triggered ROS production and consequent AMPK-p38 mediated-apoptosis. The antitumor activity was displayed when orally administered to MPM-bearing NOD/SCID mice. Conclusions: ERM and mTOR pathways are activated in MPM and 'druggable'by a combination of sorafenib and everolimus. Combination therapy is a promising therapeutic strategy against MPM. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
20. Oncogenes in non-small-cell lung cancer: emerging connections and novel therapeutic dynamics.
- Author
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Stella, Giulia M, Luisetti, Maurizio, Pozzi, Ernesto, and Comoglio, Paolo M
- Subjects
ONCOGENES ,EPITHELIAL cells ,LUNG cancer treatment ,CARCINOGENESIS ,LUNG cancer prognosis ,GENE expression ,CELLULAR signal transduction ,CLINICAL trials - Abstract
Summary: Non-small-cell lung cancer is a heterogeneous disease that is difficult to treat. Through efforts to define the molecular mechanisms involved in lung oncogenesis, molecularly targeted approaches for patients with lung cancer have now reached the clinical arena. Despite elucidation of some molecular mechanisms of lung carcinogenesis, prognosis for patients remains poor. This Review aims to highlight the functional associations between key oncogenes that drive lung tumorigenesis and are distinct targetable molecules. Oncogenes are defined by acquisition of mutations, which results in a dominant gain-of-function of the targeted protein. In this situation, a single mutated allele is sufficient to induce malignant transformation. Importantly, tumours become addicted to particular genetic alterations that cause oncogene activation and the continued expression of the signalling. An increasing amount of evidence sustains the rationale for targeting of oncogenic pathways rather than a single oncogene. A clear priority for both researchers and clinicians is to better understand the complexity of biological networks underlying lung cancer pathogenesis. This paradigmatic shift in tailoring therapies should effectively improve outcomes for patients. [ABSTRACT FROM AUTHOR]
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- 2013
- Full Text
- View/download PDF
21. Successful whole lung lavage in pulmonary alveolar proteinosis secondary to lysinuric protein intolerance: a case report.
- Author
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Ceruti, Michele, Rodi, Giuseppe, Stella, Giulia M., Adami, Andrea, Bolongaro, Antonia, Baritussio, Aldo, Pozzi, Ernesto, and Luisetti, Maurizio
- Subjects
LUNG diseases ,GENETIC disorders ,AMINO acids ,THERAPEUTICS ,CLINICAL medicine ,MEDICAL care - Abstract
Background: Pulmonary alveolar proteinosis (PAP) is a rare disease characterised by accumulation of lipoproteinaceous material within alveoli, occurring in three clinically distinct forms: congenital, acquired and secondary. Among the latter, lysinuric protein intolerance (LPI) is a rare genetic disorder caused by defective transport of cationic amino acids. Whole Lung Lavage (WLL) is currently the gold standard therapy for severe cases of PAP. Case presentation: We describe the case of an Italian boy affected by LPI who, by the age of 10, developed digital clubbing and, by the age of 16, a mild restrictive functional impairment associated with a high-resolution computed tomography (HRCT) pattern consistent with pulmonary alveolar proteinosis. After careful assessment, he underwent WLL. Conclusion: Two years after WLL, the patient has no clinical, radiological or functional evidence of pulmonary disease recurrence, thus suggesting that WLL may be helpful in the treatment of PAP secondary to LPI. [ABSTRACT FROM AUTHOR]
- Published
- 2007
- Full Text
- View/download PDF
22. Exacerbations as a starting point of pro-active chronic obstructive pulmonary disease management.
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Zoia, Maria C., Corsico, Angelo G., Beccaria, Massimiliano, Guarnone, Roberta, Cervio, Gabriella, Testi, Renato, Bressan, Maria A., Pozzi, Ernesto, and Cerveri, Isa
- Abstract
Summary: Chronic obstructive pulmonary disease (COPD) exacerbations could represent an opportunity for pro-active COPD management rather than mere treatment if previously unknown disease is discovered; the extent of underdiagnosis and undertreatment of COPD in patients attending an emergency department (ED) with an exacerbation is not known. During 2002, we recalled 131 COPD patients in stable conditions, 4–8 weeks after they had attended the ED or been discharged from our University Hospital (North-West of Italy). Information on diagnosis and management prior to the ED attendance were collected; spirometry and arterial blood gas analyses were performed. One-third of patients had never been diagnosed and treated even though 83% of them had moderate-to-very-severe COPD and about 30% already had respiratory failure. Only 20% had received information on the nature of the disease and none had received a written action plan. Only 60% were receiving long-acting bronchodilators and 41% of patients with respiratory failure were receiving long-term oxygen. A substantial number of undiagnosed and untreated patients with moderate-to-very-severe COPD came to our attention through an exacerbation. This enforces the importance of exacerbations as the starting point of pro-active COPD management and of the ED as a valuable sentinel to identify this subset of patients. [Copyright &y& Elsevier]
- Published
- 2005
- Full Text
- View/download PDF
23. SP-A binds alpha1-antitrypsin in vitro and reduces the association rate constant for neutrophil elastase.
- Author
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Gorrini, Marina, Lupi, Anna, Iadarola, Paolo, dos Santos, Conceição, Rognoni, Paola, Dalzoppo, Daniele, Carrabino, Natalia, Pozzi, Ernesto, Baritussio, Aldo, and Luisetti, Maurizio
- Subjects
TRYPSIN inhibitors ,LEUCOCYTE elastase ,OBSTRUCTIVE lung diseases ,PULMONARY emphysema ,MEDICAL research - Abstract
Background: α1-antitrypsin and surfactant protein-A (SP-A) are major lung defense proteins. With the hypothesis that SP-A could bind α1-antitrypsin, we designed a series of in vitro experiments aimed at investigating the nature and consequences of such an interaction. Methods and results: At an α1-antitrypsin:SP-A molar ratio of 1:1, the interaction resulted in a calcium-dependent decrease of 84.6% in the association rate constant of α1-antitrypsin for neutrophil elastase. The findings were similar when SP-A was coupled with the Z variant of α1-antitrypsin. The carbohydrate recognition domain of SP-A appeared to be a major determinant of the interaction, by recognizing α1-antitrypsin carbohydrate chains. However, binding of SP-A carbohydrate chains to the α1-antitrypsin amino acid backbone and interaction between carbohydrates of both proteins are also possible. Gel filtration chromatography and turnover per inactivation experiments indicated that one part of SP-A binds several molar parts of α1-antitrypsin. Conclusion: We conclude that the binding of SP-A to α1-antitrypsin results in a decrease of the inhibition of neutrophil elastase. This interaction could have potential implications in the physiologic regulation of α1-antitrypsin activity, in the pathogenesis of pulmonary emphysema, and in the defense against infectious agents. [ABSTRACT FROM AUTHOR]
- Published
- 2005
- Full Text
- View/download PDF
24. Primary human mesothelioma cells express class II MHC, ICAM-1 and B7-2 and can present recall antigens to autologous blood lymphocytes.
- Author
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Mutti, Luciano, Valle, Maria T., Balbi, Bruno, Orengo, Anna M., Lazzaro, Antonio, Alciato, Paolo, Gatti,, Emiliano, Betta, Pier G., and Pozzi, Ernesto
- Published
- 1998
- Full Text
- View/download PDF
25. Clinical efficacy of dirithromycin versus miocamycin in the treatment of acute bronchitis or acute exacerbations of chronic bronchitis.
- Author
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Pozzi, Ernesto
- Abstract
A multicentre trial was carried out to compare the efficacy and safety of dirithromycin with miocamycin in the treatment of patients with acute bronchitis or acute exacerbations of chronic bronchitis. The study was a single-blind, randomized, parallel-group study. Dirithromycin was administered orally at a dosage of 500 mg once daily and miocamycin was administered orally at a dosage of 600 mg twice daily; the duration of therapy was five to seven days for both drugs. The results, in 161 assessable patients (78 taking dirithromycin; 83 taking miocamycin), show that dirithromycin and miocamycin have comparable efficacy and safety in the treatment of bronchitis caused by susceptible bacterial pathogens. [ABSTRACT FROM PUBLISHER]
- Published
- 1993
26. Relationship Between Diffuse Pulmonary Fibrosis, Alveolar Proteinosis, and Granulocyte-Macrophage Colony Stimulating Factor Autoantibodies.
- Author
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Luisetti, Maurizio, Bruno, Pierdonato, Kadija, Zamir, Suzuki, Takuji, Raffa, Salvatore, Torrisi, Maria Rosaria, Campo, Ilaria, Mariani, Francesca, Pozzi, Ernesto, Trapnell, Bruce C., and Mariotta, Salvatore
- Subjects
LUNG radiography ,PREDNISONE ,PULMONARY fibrosis treatment ,BRONCHOALVEOLAR lavage ,DYSPNEA ,GRANULOCYTE-macrophage colony-stimulating factor ,LUNG diseases ,OXYGEN therapy ,PULMONARY fibrosis ,PULMONARY function tests ,PULMONARY gas exchange ,PULMONARY surfactant ,RESPIRATORY insufficiency ,DIAGNOSIS - Abstract
Extensive pulmonary fibrosis is a rare occurrence in pulmonary alveolar proteinosis. We report 2 cases that have interesting implications. A female patient was diagnosed with autoimmune pulmonary alveolar proteinosis that evolved over 7 years into diffuse fibrosis. In a male patient with diffuse fibrosis we incidentally detected electron microscopic features of alveolar surfactant accumulation and positive autoantibodies to granulocyte-macrophage colony stimulating factor. In the male patient we speculated that the pulmonary fibrosis might have been preceded by an asymptomatic phase of autoimmune pulmonary alveolar proteinosis, and that we should investigate the involvement of surfactant dysfunction in the pathogenesis of fibrotic lung disease. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
27. “Nonobstructive” emphysema of the lung.
- Author
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Corsico, Angelo G., Niniano, Rosanna, Gatto, Elena, Zoia, Maria C., Corsico, Andrea, Cremaschi, Paolo, Pozzi, Ernesto, and Cerveri, Isa
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OBSTRUCTIVE lung diseases ,PULMONARY hypertension ,BLOOD circulation disorders ,PULMONARY function tests - Abstract
Summary: An unusual case of smoking-related centrilobular emphysema with normal spirometry. A 64-year-old man presented with severe dyspnoea and respiratory failure. Pulmonary function and mechanics were normal except for a marked reduction in diffusing capacity of the lung. High-resolution CT scan showed diffuse centrilobular emphysema also involving lower lobes. Pulmonary embolism, cardiac or pulmonary shunt and immunopathologically based vasculitis were excluded. Pulmonary pressure was at the upper limit of normality but within few months he developed a severe pulmonary hypertension. Although spirometry is the only physiologic measure recommended by the updated Global Initiative for Chronic Obstructive Lung Disease guidelines for confirming the diagnosis it should be recognized that diffuse emphysema may occur with only abnormalities in gas exchange without airflow obstruction. The identification of different phenotypes within COPD is important for understanding disease heterogeneity and progression. [Copyright &y& Elsevier]
- Published
- 2007
- Full Text
- View/download PDF
28. Estrogen and progesterone receptors in women with non-small-cell lung cancer: a potential therapeutic target?
- Author
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Bencardino, Katia, Manzoni, Mariangela, Mariucci, Sara, Morbini, Patrizia, Delfanti, Sara, Chatzileontiadou, Sofia, Rovati, Bianca, Nascimbene, Caterina, Pozzi, Ernesto, and Danova, Marco
- Published
- 2007
- Full Text
- View/download PDF
29. Prognostic significance of Ki67 labelling in resected non small cell lung cancer
- Author
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Scagliotti, Giorgio Vittorio, Micela, Marco, Gubetta, Luciano, Leonardo, Eugenio, Cappia, Susanna, Borasio, Piero, and Pozzi, Ernesto
- Published
- 1993
- Full Text
- View/download PDF
30. Systemic inflammatory response and downmodulation of peripheral CD25+Foxp3+ T-regulatory cells in patients undergoing radiofrequency thermal ablation for lung cancer
- Author
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Fietta, Anna Maria, Morosini, Monica, Passadore, Ileana, Cascina, Alessandro, Draghi, Paola, Dore, Roberto, Rossi, Sandro, Pozzi, Ernesto, and Meloni, Federica
- Subjects
- *
IMMUNOLOGY of inflammation , *RADIO frequency , *THERMOTHERAPY , *LUNG cancer treatment , *T cell receptors , *INTERLEUKIN-2 , *GENETIC regulation , *CELL proliferation , *TREATMENT effectiveness - Abstract
Abstract: Radiofrequency thermal ablation (RFTA) is a local tumor-destructing technique that can potentially modulate the host immune response through mechanisms that are not clearly defined. We assessed whether RFTA could affect multiple systemic inflammatory and immunological parameters, including CD25+Foxp3+ cells, in patients with primary or metastatic lung tumors. Three days after RFTA, a moderate and temporary systemic inflammatory response developed, as demonstrated by the increase in peripheral neutrophils and monocytes and in plasma levels of proinflammatory chemokines (MIP-1α, MIP-1β, eotaxin, and interleukin[IL]-8) and acute phase reactants (complement C3 and C4, serum amyloid, α1 antichymotrypsin, and C-reactive protein). Moreover, we found a concomitant release of the anti-inflammatory factor IL-10. Thirty days after RFTA, a significant reduction in CD25+Foxp3+ counts with an increase in CD4+ T-cell proliferation and number of interferon-γ-secreting cells was observed. The reduction in CD25+Foxp3+ cells lasted up to 90 days after treatment. The use of RFTA in lung cancer patients has an immunomodulatory activity: it induces a self-limiting systemic inflammation early and later a reduction of circulating CD25+Foxp3+ Tregs. In addition to tumor ablation, downmodulation of this regulatory subset might be an important mechanism involved in the long-term clinical efficacy of RFTA. [Copyright &y& Elsevier]
- Published
- 2009
- Full Text
- View/download PDF
31. Bronchoalveolar Lavage Fluid Proteome in Bronchiolitis Obliterans Syndrome: Possible Role for Surfactant Protein A in Disease Onset
- Author
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Meloni, Federica, Salvini, Roberta, Bardoni, Anna Maria, Passadore, Ileana, Solari, Nadia, Vitulo, Patrizio, Oggionni, Tiberio, Viganò, Mario, Pozzi, Ernesto, and Fietta, Anna Maria
- Subjects
- *
BRONCHOALVEOLAR lavage , *BRONCHIOLE diseases , *LUNG transplantation , *MASS spectrometry , *GEL electrophoresis , *PATIENTS - Abstract
Background: Bronchiolitis obliterans syndrome (BOS) affects long-term survival of lung transplant (Tx) recipients (LTRs), with no consistently effective treatment strategy. Identifying early markers of BOS is of paramount importance for improving graft survival. Methods: We used 2-dimensional gel electrophoresis and protein identification by mass spectrometry to compare the protein profile of bronchoalveolar lavage fluid (BALf) in two groups of LTRs: one composed of patients with BOS and the other composed of patients with good graft function at >5 years post-surgery (stable LTRs). Based on the hypothesis that only proteins of lung origin could represent reliable BOS markers, we also evaluated paired plasma samples. Proteins of interest were also assessed in the BALf of control subjects and results confirmed by dot- blot analysis. Results: Among 11 differentially expressed proteins, we identified 2 locally produced factors: peroxiredoxin II (PRXII), exclusively expressed in BOS; and surfactant protein A (SP-A), expressed consistently less in BOS patients than in stable LTRs. PRXII expression was never observed in BALf from control subjects, whereas SP-A was present in higher amounts compared with stable LTRs and BOS patients. Finally, the time course of SP-A was studied in 5 LTRs who subsequently developed BOS. A reduction in BALf SP-A content was detectable early after Tx, preceding BOS onset in 4 of 5 patients. Conclusions: Our results suggest that testing SP-A levels in BALf could predict LTR patients who are at higher risk of BOS development. [Copyright &y& Elsevier]
- Published
- 2007
- Full Text
- View/download PDF
32. Foxp3 Expressing CD4+ CD25+ and CD8+CD28− T Regulatory Cells in the Peripheral Blood of Patients with Lung Cancer and Pleural Mesothelioma
- Author
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Meloni, Federica, Morosini, Monica, Solari, Nadia, Passadore, Ileana, Nascimbene, Caterina, Novo, Monique, Ferrari, Marco, Cosentino, Marco, Marino, Franca, Pozzi, Ernesto, and Fietta, Anna M.
- Subjects
- *
LUNG cancer , *MESOTHELIOMA , *T cells , *SUPPRESSOR cells , *IMMUNOSUPPRESSION - Abstract
Abstract: The role of T regulatory (Treg) cells in human cancer has not yet been clarified. We assessed the presence and function of CD4+ and CD8+ Treg cell subsets in the peripheral blood of patients with lung cancer (LC) and pleural mesothelioma (PM). We found a low but significant increase in the number of CD4+ T cells with phenotype and functional features of Treg cells in LC patients compared to normal healthy controls (NHC). Furthermore, total CD4+ T cells from LC patients proliferated less than cells from controls, suggesting that the increase in the CD4+ Treg cell pool has functional importance. LC patients also showed an expansion of the CD8+CD28− T cell subset and these cells expressed Foxp3 mRNA, as recently observed in alloantigen-specific CD8+CD28− T suppressor cells. No variation of peripheral Treg cell subsets was found in patients with PM, a disease with a predominantly localized nature. However, the lack of correlation between cancer stage and the number or the function of peripheral Treg cells in LC patients refuted the hypothesis that these cells are involved in tumor spreading. A possible involvement of the peripheral Treg cell pool in cancer development and/or in inducing systemic immunosuppression in LC patients can be hypothesized. [Copyright &y& Elsevier]
- Published
- 2006
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33. PHARMACOLOGICAL ANALYSIS OF SIGNAL TRANSDUCTION PATHWAYS REQUIRED FOR MYCOBACTERIUM TUBERCULOSIS -INDUCED IL-8 AND MCP-1 PRODUCTION IN HUMAN PERIPHERAL MONOCYTES
- Author
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Fietta, Anna M, Morosini, Monica, Meloni, Federica, Bianco, Alessia Marone, and Pozzi, Ernesto
- Subjects
- *
MONOCYTES , *INTERLEUKIN-8 , *MYCOBACTERIUM tuberculosis - Abstract
Signalling cascades involved in chemokine production by human phagocytes following infection withMycobacterium tuberculosis are still not defined. We used specific pharmacologic inhibitors to identify the signalling molecules which lead to interleukin (IL)-8 and MCP-1 production in human monocytes in response to M. tuberculosis infection. Inhibition of extracellular signal-regulated (ERK) or p38 mitogen-activated protein kinase by PD 98059 and SB 203580 respectively, significantly affected chemokine production. However, only the presence of both inhibitors completely blocked the release. A down-regulation of chemokine secretion was found in presence of inhibitors of protein kinase (PK)C and phospholipase C. Moreover, production depended on transcription activation via the nuclear factor-kappa B (NF-κB), as demonstrated by treatment with actinomycin D and caffeic acid phenethyl ester. In addition, activation of PKA and the phosphoinoside 3-kinase (PI-3k)/p70 ribosomal S6 kinase cascade was required to have maximal MCP-1 but not IL-8 production. In conclusion, this study provides evidence that multiple signal transduction pathways are involved in M. tuberculosis -induced chemokine secretion by human monocytes. Moreover, for the first time this report indicates that inhibitors of some signalling molecules are able to dissociate IL-8 from MCP-1 secretion. Differences in the regulatory pathways of chemokine production can potentially be exploited therapeutically. [Copyright &y& Elsevier]
- Published
- 2002
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34. Non-neuronal cholinergic system in airways and lung cancer susceptibility.
- Author
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Saracino L, Zorzetto M, Inghilleri S, Pozzi E, and Stella GM
- Abstract
In the airway tract acetylcholine (ACh) is known to be the mediator of the parasympathetic nervous system. However ACh is also synthesized by a large variety of non-neuronal cells. Strongest expression is documented in neuroendocrine and in epithelial cells (ciliated, basal and secretory elements). Growing evidence suggests that a cell-type specific Ach expression and release do exist and act with local autoparacrine loop in the non-neuronal airway compartment. Here we review the molecular mechanism by which Ach is involved in regulating various aspects of innate mucosal defense, including mucociliary clearance, regulation of macrophage activation as well as in promoting epithelial cells proliferation and conferring susceptibility to lung carcinoma onset. Importantly this non-neuronal cholinergic machinery is differently regulated than the neuronal one and could be specifically therapeutically targeted.
- Published
- 2013
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35. Synchronous lung cancers: when same histological types feature different molecular profiles and response phenotypes.
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Stella GM, Cemmi F, Inghilleri S, Zorzetto M, Luisetti M, and Pozzi E
- Abstract
We discuss the case of synchronous bilateral lung cancers which feature the same histological phenotype and a different EGFR mutational profile. Both histological and molecular characterizations were performed on specimens derived thorough CT-guided fine needle aspiration. A first-line chemotherapy was unsuccessful. Subsequent objective response to the EGFR inhibitor Erlotinib was clearly coherent with the sequencing data and the mutated nodule was effectively reduced (> 50%) after therapy, while the lesion assessed as EGFR wild type featured a slight response. This report has two relevant implications. It points out that in case of multiple malignant lesions at time of diagnosis, molecular profiling should be as extensive as possible and it might contribute to clarify the association between the lesions found. Besides the molecular analysis on cytology specimens could identify an accurate and safe diagnostic approach for clinical use.
- Published
- 2011
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- View/download PDF
36. Factors influencing oxidative imbalance in pulmonary fibrosis: an immunohistochemical study.
- Author
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Inghilleri S, Morbini P, Campo I, Zorzetto M, Oggionni T, Pozzi E, and Luisetti M
- Abstract
Background. Idiopathic Pulmonary Fibrosis (IPF) is a fatal lung disease of unknown etiology characterized by interstitial fibrosis determining irreversible distortion of pulmonary architecture. Reactive oxygen species (ROS) and markers of oxidative stress play a pivotal role in human IPF pathology, possibly through induction of epithelial-mesenchymal transition (EMT). Methods. We investigated by immunohistochemistry, in UIP and COP tissue samples, the expression of most relevant markers of the molecular interplay involving RAGE, oxidant/antioxidant balance regulation, tissue nitrosylation, and mediators of EMT. Results. In both UIP and COP, the degree of RAGE expression was similarly high, while SODs and i-NOS, diffusely present in COP endoalveolar plugs, were almost absent in UIP fibroblast foci. A lower degree of tissue nitrosilation was observed in UIP than in COP. Conclusions. Fibroblast lesions of UIP and of COP share a similar degree of activation of RAGE, while antioxidant enzyme expression markedly reduced in UIP.
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- 2011
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37. Systemic inflammatory response and downmodulation of peripheral CD25+Foxp3+ T-regulatory cells in patients undergoing radiofrequency thermal ablation for lung cancer.
- Author
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Fietta AM, Morosini M, Passadore I, Cascina A, Draghi P, Dore R, Rossi S, Pozzi E, and Meloni F
- Subjects
- Acute-Phase Proteins analysis, Aged, 80 and over, Animals, Blood Proteins analysis, Cell Proliferation, Cytokines blood, Down-Regulation, Electrophoresis, Gel, Two-Dimensional, Enzyme-Linked Immunosorbent Assay, Female, Flow Cytometry, Forkhead Transcription Factors blood, Humans, Interleukin-2 Receptor alpha Subunit blood, Lung Neoplasms pathology, Lymphocyte Count, Lymphocyte Subsets cytology, Lymphocyte Subsets metabolism, Male, Middle Aged, T-Lymphocytes, Regulatory metabolism, Time Factors, Treatment Outcome, Catheter Ablation methods, Inflammation Mediators blood, Lung Neoplasms blood, Lung Neoplasms surgery, T-Lymphocytes, Regulatory cytology
- Abstract
Radiofrequency thermal ablation (RFTA) is a local tumor-destructing technique that can potentially modulate the host immune response through mechanisms that are not clearly defined. We assessed whether RFTA could affect multiple systemic inflammatory and immunological parameters, including CD25+Foxp+ cells, in patients with primary or metastatic lung tumors. Three days after RFTA, a moderate and temporary systemic inflammatory response developed, as demonstrated by the increase in peripheral neutrophils and monocytes and in plasma levels of proinflammatory chemokines (MIP-1alpha, MIP-1beta, eotaxin, and interleukin[IL]-8) and acute phase reactants (complement C3 and C4, serum amyloid, alpha1 antichymotrypsin, and C-reactive protein). Moreover, we found a concomitant release of the anti-inflammatory factor IL-10. Thirty days after RFTA, a significant reduction in CD25+Foxp3+ counts with an increase in CD4+ T-cell proliferation and number of interferon-gamma-secreting cells was observed. The reduction in CD25+Foxp3+ cells lasted up to 90 days after treatment. The use of RFTA in lung cancer patients has an immunomodulatory activity: it induces a self-limiting systemic inflammation early and later a reduction of circulating CD25+Foxp3+ Tregs. In addition to tumor ablation, downmodulation of this regulatory subset might be an important mechanism involved in the long-term clinical efficacy of RFTA.
- Published
- 2009
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38. Foxp3 expressing CD4+ CD25+ and CD8+CD28- T regulatory cells in the peripheral blood of patients with lung cancer and pleural mesothelioma.
- Author
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Meloni F, Morosini M, Solari N, Passadore I, Nascimbene C, Novo M, Ferrari M, Cosentino M, Marino F, Pozzi E, and Fietta AM
- Subjects
- Aged, CD28 Antigens analysis, CD4 Antigens analysis, CD8 Antigens analysis, CD8-Positive T-Lymphocytes classification, Female, Forkhead Transcription Factors genetics, Humans, Immune Tolerance, Lung Neoplasms pathology, Male, Mesothelioma pathology, Middle Aged, Pleural Neoplasms pathology, Receptors, Interleukin-2 analysis, T-Lymphocyte Subsets immunology, T-Lymphocytes, Regulatory classification, CD8-Positive T-Lymphocytes immunology, Forkhead Transcription Factors metabolism, Lung Neoplasms immunology, Mesothelioma immunology, Pleural Neoplasms immunology, T-Lymphocytes, Regulatory immunology
- Abstract
The role of T regulatory (Treg) cells in human cancer has not yet been clarified. We assessed the presence and function of CD4+ and CD8+ Treg cell subsets in the peripheral blood of patients with lung cancer (LC) and pleural mesothelioma (PM). We found a low but significant increase in the number of CD4+ T cells with phenotype and functional features of Treg cells in LC patients compared to normal healthy controls (NHC). Furthermore, total CD4+ T cells from LC patients proliferated less than cells from controls, suggesting that the increase in the CD4+ Treg cell pool has functional importance. LC patients also showed an expansion of the CD8+CD28- T cell subset and these cells expressed Foxp3 mRNA, as recently observed in alloantigen-specific CD8+CD28- T suppressor cells. No variation of peripheral Treg cell subsets was found in patients with PM, a disease with a predominantly localized nature. However, the lack of correlation between cancer stage and the number or the function of peripheral Treg cells in LC patients refuted the hypothesis that these cells are involved in tumor spreading. A possible involvement of the peripheral Treg cell pool in cancer development and/or in inducing systemic immunosuppression in LC patients can be hypothesized.
- Published
- 2006
- Full Text
- View/download PDF
39. SP-A binds alpha1-antitrypsin in vitro and reduces the association rate constant for neutrophil elastase.
- Author
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Gorrini M, Lupi A, Iadarola P, Dos Santos C, Rognoni P, Dalzoppo D, Carrabino N, Pozzi E, Baritussio A, and Luisetti M
- Subjects
- Enzyme Activation, Kinetics, Protein Binding, Leukocyte Elastase chemistry, Pulmonary Surfactant-Associated Protein A chemistry, alpha 1-Antitrypsin chemistry
- Abstract
Background: Alpha1-antitrypsin and surfactant protein-A (SP-A) are major lung defense proteins. With the hypothesis that SP-A could bind alpha1-antitrypsin, we designed a series of in vitro experiments aimed at investigating the nature and consequences of such an interaction., Methods and Results: At an alpha1-antitrypsin:SP-A molar ratio of 1:1, the interaction resulted in a calcium-dependent decrease of 84.6% in the association rate constant of alpha1-antitrypsin for neutrophil elastase. The findings were similar when SP-A was coupled with the Z variant of alpha1-antitrypsin. The carbohydrate recognition domain of SP-A appeared to be a major determinant of the interaction, by recognizing alpha1-antitrypsin carbohydrate chains. However, binding of SP-A carbohydrate chains to the alpha1-antitrypsin amino acid backbone and interaction between carbohydrates of both proteins are also possible. Gel filtration chromatography and turnover per inactivation experiments indicated that one part of SP-A binds several molar parts of alpha1-antitrypsin., Conclusion: We conclude that the binding of SP-A to alpha1-antitrypsin results in a decrease of the inhibition of neutrophil elastase. This interaction could have potential implications in the physiologic regulation of alpha1-antitrypsin activity, in the pathogenesis of pulmonary emphysema, and in the defense against infectious agents.
- Published
- 2005
- Full Text
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40. NOD2/CARD15 gene polymorphisms in idiopathic pulmonary fibrosis.
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Zorzetto M, Ferrarotti I, Campo I, Trisolini R, Poletti V, Scabini R, Ceruti M, Mazzola P, Crippa E, Ottaviani S, Agostini C, Semenzato G, Pozzi E, and Luisetti M
- Subjects
- Aged, Amino Acid Substitution, DNA Primers, Female, Genetic Carrier Screening, Genotype, Homozygote, Humans, Italy, Male, Middle Aged, Nod2 Signaling Adaptor Protein, Reference Values, Sarcoidosis genetics, White People, Intracellular Signaling Peptides and Proteins genetics, Polymorphism, Single Nucleotide, Pulmonary Fibrosis genetics
- Abstract
Background: Micro-organisms, behaving in a non-infectious fashion, may be among the exogenous factor(s) believed to trigger idiopathic pulmonary fibrosis (IPF). One possible strategy to identify an individual's susceptibility to such microbial triggers, which are likely to be ubiquitous, is to investigate the molecular processes involved in their recognition. NOD2/CARD15 is a specific pattern recognition receptor protein, whose genetic variants have been previously associated with susceptibility to Crohn's disease., Aim: The aim of this work was to determine the frequencies of the three major NOD2/CARD 15 gene mutations (R702W, G908R and 1007fsinC) in a series of 76 subjects affected by IPF, and to compare them with those found in three groups of controls: a group with sarcoidosis (a disorder in which an involvement of the NOD2/CARD15 gene has already been investigated and rejected in different ethnic groups; 67 subjects) and two groups of healthy subjects (218 and 208 subjects, respectively), matched for gender, age, and ethnicity., Results: We found no differences in frequencies of NOD2/CARD15 gene polymorphisms among the four groups investigated., Conclusion: We conclude that the NOD2/CARD15 gene is not likely to be involved in susceptibility to IPF in Italians.
- Published
- 2005
41. BAL cytokine profile in different interstitial lung diseases: a focus on systemic sclerosis.
- Author
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Meloni F, Caporali R, Marone Bianco A, Paschetto E, Morosini M, Fietta AM, Patrizio V, Bobbio-Pallavicini F, Pozzi E, and Montecucco C
- Subjects
- Adult, Aged, Bronchoalveolar Lavage Fluid chemistry, Cytokines immunology, Female, Humans, Lung Diseases, Interstitial diagnosis, Lung Diseases, Interstitial immunology, Male, Middle Aged, Predictive Value of Tests, Pulmonary Fibrosis diagnosis, Sarcoidosis, Pulmonary diagnosis, Scleroderma, Systemic complications, Scleroderma, Systemic diagnosis, Bronchoalveolar Lavage Fluid immunology, Cytokines analysis, Pulmonary Fibrosis immunology, Sarcoidosis, Pulmonary immunology, Scleroderma, Systemic immunology
- Abstract
Background and Aim: Fibrosing alveolitis develops in up to 80% of systemic sclerosis patients (SSc) but progression to end stage fibrosis occurs in about 15% of cases. Mechanisms leading to the process remain mostly unknown. We compared cytokine profiles of broncho-alveolar lavage fluids (BAL-f) from patients with SSc associated interstitial lung disease (SSc-ILD) (n. 34), idiopathic pulmonary fibrosis (IPF) (n. 13), stage II sarcoidosis (n. 14) and 9 controls., Methods: Interleukin (IL) 8, monocyte chemoattractant protein 1 (MCP-1), gamma-interferon (IFN-gamma), IL12, IL18 and IL10 and transforming growth factor-beta (TGF-beta) were assessed by ELISA in concentrated BAL-f., Results: Levels of IL8 and MCP-1 were significantly elevated in SSc-ILD and in IPF as compared with controls (Mann Whitney test p < 0.05), while MCP-1 values were significantly lower in SSc-ILD than in IPF. A significant correlation between neutrophils and IL8 levels (p = 0.047), as well as between eosinophils and MCP-1 levels (p = 0.004) was also observed. IFN-gamma levels were slightly higher than normal only in sarcoidosis (p = 0.06), whereas IL12 levels increased both in sarcoidosis and SSc-ILD (p < 0.05). No differences were found in IL18 and TGF-beta levels. Finally, IL10 levels were higher in SSc-ILD and sarcoidosis than in controls and IPF (p < 0.05)., Conclusion: BAL-f cytokine profile differentiates ILD associated with SSc from IPF. The lower expression of MCP-1 and the higher expression of the anti-fibrotic IL12 and the anti-inflammatory IL10, observed both in sarcoidosis and in SSc-ILD, could account for the better prognosis of these ILDs. Further longitudinal studies are required to confirm whether a different cytokine phenotype may be considered predictive of clinical outcome in SSc-ILD.
- Published
- 2004
42. Regulatory CD4+CD25+ T cells in the peripheral blood of lung transplant recipients: correlation with transplant outcome.
- Author
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Meloni F, Vitulo P, Bianco AM, Paschetto E, Morosini M, Cascina A, Mazzucchelli I, Ciardelli L, Oggionni T, Fietta AM, Pozzi E, and Viganò M
- Subjects
- Adult, Aged, Bronchiolitis Obliterans immunology, CD4 Lymphocyte Count, CD4-Positive T-Lymphocytes chemistry, Female, Graft Rejection immunology, Humans, Male, Middle Aged, Receptors, Interleukin-2 analysis, Treatment Outcome, CD4-Positive T-Lymphocytes cytology, CD4-Positive T-Lymphocytes immunology, Lung Transplantation immunology
- Abstract
Background: The subset of CD4+CD25+ regulatory T cells, recently identified in humans, may play a central role in the regulation of immune tolerance to graft survival., Methods: This study assesses the frequency and functional profile of CD4+CD25+CD69- cells in the peripheral blood of lung transplant recipients (>3 years from transplantation), 10 of whom were in a stable clinical condition and 11 of whom demonstrated chronic rejection (bronchiolitis obliterans syndrome). We also studied a group of seven healthy subjects., Results: The frequency of CD4+ T cells expressing CD25 (CD4+CD25+) and the highest levels (CD25) were lower in patients with bronchiolitis obliterans syndrome compared with healthy subjects and subjects in a stable clinical condition (P < or = 0.01). Purified CD4+CD25+ cells exhibited a regulatory functional profile in vitro: they were hyporesponsive, suppressed the proliferation of CD4+CD25- cells, and produced interleukin-10., Conclusion: These results provide in vivo evidence that peripheral CD4+CD25+ T cells may represent an important regulatory subset in lung transplantation.
- Published
- 2004
- Full Text
- View/download PDF
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