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516 results on '"Pizza, Mariagrazia"'

3. Scientists’ call to action: Microbes, planetary health, and the Sustainable Development Goals

Author

Crowther, Thomas W., Rappuoli, Rino, Corinaldesi, Cinzia, Danovaro, Roberto, Donohue, Timothy J., Huisman, Jef, Stein, Lisa Y., Timmis, James Kenneth, Timmis, Kenneth, Anderson, Matthew Z., Bakken, Lars R., Baylis, Matthew, Behrenfeld, Michael J., Boyd, Philip W., Brettell, Ian, Cavicchioli, Ricardo, Delavaux, Camille S., Foreman, Christine M., Jansson, Janet K., Koskella, Britt, Milligan-McClellan, Kat, North, Justin A., Peterson, Devin, Pizza, Mariagrazia, Ramos, Juan L., Reay, David, Remais, Justin V., Rich, Virginia I., Ripple, William J., Singh, Brajesh K., Smith, Gabriel Reuben, Stewart, Frank J., Sullivan, Matthew B., van den Hoogen, Johan, van Oppen, Madeleine J.H., Webster, Nicole S., Zohner, Constantin M., and van Galen, Laura G.

Published
2024
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4. A next-generation GMMA-based vaccine candidate to fight shigellosis

Author

Rossi, Omar, Citiulo, Francesco, Giannelli, Carlo, Cappelletti, Emilia, Gasperini, Gianmarco, Mancini, Francesca, Acquaviva, Alessandra, Raso, Maria Michelina, Sollai, Luigi, Alfini, Renzo, Aruta, Maria Grazia, Vitali, Claudia Giorgina, Pizza, Mariagrazia, Necchi, Francesca, Rappuoli, Rino, Martin, Laura B., Berlanda Scorza, Francesco, Colucci, Anna Maria, and Micoli, Francesca

Published
2023
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8. Urgent Need to Understand and Prevent Gonococcal Infection: From the Laboratory to Real-World Context.

Author

García, Yara Ruiz, Marrazzo, Jeanne, Martinón-Torres, Federico, Workowski, Kimberly, Giordano, Giulia, Pizza, Mariagrazia, and Sohn, Woo-Yun

Subjects
EXTRACELLULAR vesicles, NEISSERIA gonorrhoeae, NEISSERIA meningitidis, VACCINE effectiveness, CELL surface antigens
Abstract

Neisseria gonorrhoeae is widespread globally. Primary prevention is unsuccessful and antimicrobial resistance threatens optimal management. There is no specific vaccine and natural infection studies show that N gonorrhoeae can avoid and suppress immune responses. In addition to extensive variation in expression and specificity of many gonococcal surface antigens, it induces a robust inflammatory response through the Th17 pathway with a large influx of neutrophils and inflammatory cytokines but evades macrophages. The Th1- and Th2-mediated response is suppressed, resulting in low, short-lived antibody titers. Real-world evidence suggests that gonorrhea cases are reduced among recipients of Neisseria meningitidis group B vaccines containing outer membrane vesicles (OMVs). Although the first randomized trial of an OMV-containing MenB vaccine against N gonorrhoeae infection did not show statistically significant vaccine efficacy, ongoing trials might shed further light. Several candidate vaccine antigens for a gonococcal-specific vaccine are being evaluated preclinically but only one has reached clinical trials. [ABSTRACT FROM AUTHOR]

Published
2024
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15. Development of a visual Adhesion/Invasion Inhibition Assay to assess the functionality of Shigella-specific antibodies.

Author

Batani, Giampiero, Vezzani, Giacomo, Lashchuk, Sabrina, Allaoui, Abdelmounaaim, Cardamone, Dario, Raso, Maria Michelina, Boero, Elena, Roscioli, Emanuele, Ridelfi, Matteo, Gasperini, Gianmarco, Pizza, Mariagrazia, Rossi, Omar, Scorza, Francesco Berlanda, Micoli, Francesca, Rappuoli, Rino, and Sala, Claudia

Subjects
SHIGELLOSIS, BACTERIAL antibodies, BACTERIAL adhesion, IMMUNOGLOBULINS, EPITHELIAL cells
Abstract

Introduction: Shigella is the etiologic agent of a bacillary dysentery known as shigellosis, which causes millions of infections and thousands of deaths worldwide each year due to Shigella's unique lifestyle within intestinal epithelial cells. Cell adhesion/invasion assays have been extensively used not only to identify targets mediating host-pathogen interaction, but also to evaluate the ability of Shigella-specific antibodies to reduce virulence. However, these assays are time-consuming and labor-intensive and fail to assess differences at the single-cell level. Objectives and methods: Here, we developed a simple, fast and high-content method named visual Adhesion/Invasion Inhibition Assay (vAIA) to measure the ability of anti-Shigella antibodies to inhibit bacterial adhesion to and invasion of epithelial cells by using the confocal microscope Opera Phenix. Results: We showed that vAIA performed well with a pooled human serum from subjects challenged with S. sonnei and that a specific anti-IpaD monoclonal antibody effectively reduced bacterial virulence in a dose-dependent manner. Discussion: vAIA can therefore inform on the functionality of polyclonal and monoclonal responses thereby supporting the discovery of pathogenicity mechanisms and the development of candidate vaccines and immunotherapies. Lastly, this assay is very versatile and may be easily applied to other Shigella species or serotypes and to different pathogens. [ABSTRACT FROM AUTHOR]

Published
2024
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16. Enhancing a multi-purpose artificial urine for culture and gene expression studies of uropathogenic Escherichia coli strains.

Author

Rimbi, Patricia T, O'Boyle, Nicky, Douce, Gillian R, Pizza, Mariagrazia, Rosini, Roberto, and Roe, Andrew J

Subjects
LIQUID chromatography-mass spectrometry, GENE expression, ESCHERICHIA coli, URODYNAMICS, URINE, GENE expression profiling
Abstract

Aims The main objective of this study was to modify a recently reported multi-purpose artificial urine (MP-AU) for culture and gene expression studies of uropathogenic Escherichia coli (UPEC) strains. Methods and results We used liquid chromatography mass spectrometry (LC-MS) to identify and adjust the metabolic profile of MP-AU closer to that of pooled human urine (PHU). Modification in this way facilitated growth of UPEC strains with growth rates similar to those obtained in PHU. Transcriptomic analysis of UPEC strains cultured in enhanced artificial urine (enhanced AU) and PHU showed that the gene expression profiles are similar, with <7% of genes differentially expressed between the two conditions. Conclusions Enhancing an MP-AU with metabolites identified in PHU allows the enhanced AU to be used as a substitute for the culture and in vitro gene expression studies of UPEC strains. [ABSTRACT FROM AUTHOR]

Published
2024
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20. Meningococcal serogroup B strain coverage of the multicomponent 4CMenB vaccine with corresponding regional distribution and clinical characteristics in England, Wales, and Northern Ireland, 2007–08 and 2014–15: a qualitative and quantitative assessment

Author

Parikh, Sydel R, Newbold, Lynne, Slater, Stephanie, Stella, Maria, Moschioni, Monica, Lucidarme, Jay, De Paola, Rosita, Giuliani, Maria, Serino, Laura, Gray, Stephen J, Clark, Stephen A, Findlow, Jamie, Pizza, Mariagrazia, Ramsay, Mary E, Ladhani, Shamez N, and Borrow, Ray

Published
2017
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21. Identification of Vaccine Candidates against Serogroup B Meningococcus by Whole-Genome Sequencing

Author

Pizza, Mariagrazia, Scarlato, Vincenzo, Masignani, Vega, Giuliani, Marzia Monica, Aricò, Beatrice, Comanducci, Maurizio, Jennings, Gary T., Baldi, Lucia, Bartolini, Erika, Capecchi, Barbara, Galeotti, Cesira L., Luzzi, Enrico, Manetti, Roberto, Marchetti, Elisa, Mora, Marirosa, Nuti, Sandra, Ratti, Giulio, Santini, Laura, Savino, Silvana, Scarselli, Maria, Storni, Elisa, Zuo, Peijun, Broeker, Michael, Hundt, Erika, Knapp, Bernard, Blair, Eric, Mason, Tanya, Tettelin, Hervé, Hood, Derek W., Jeffries, Alex C., Saunders, Nigel J., Granoff, Dan M., Venter, J. Craig, Moxon, E. Richard, Grandi, Guido, and Rappuoli, Rino

Published
2000

22. Complete Genome Sequence of Neisseria meningitidis Serogroup B Strain MC58

Author

Tettelin, Hervé, Saunders, Nigel J., Heidelberg, John, Jeffries, Alex C., Nelson, Karen E., Eisen, Jonathan A., Ketchum, Karen A., Hood, Derek W., Peden, John F., Dodson, Robert J., Nelson, William C., Gwinn, Michelle L., DeBoy, Robert, Peterson, Jeremy D., Hickey, Erin K., Haft, Daniel H., Salzberg, Steven L., White, Owen, Fleischmann, Robert D., Dougherty, Brian A., Mason, Tanya, Ciecko, Anne, Parksey, Debbie S., Blair, Eric, Cittone, Henry, Clark, Emily B., Cotton, Matthew D., Utterback, Terry R., Khouri, Hoda, Qin, Haiying, Vamathevan, Jessica, Gill, John, Scarlato, Vincenzo, Masignani, Vega, Pizza, Mariagrazia, Grandi, Guido, Sun, Li, Smith, Hamilton O., Fraser, Claire M., Moxon, E. Richard, Rappuoli, Rino, and Venter, J. Craig

Published
2000

24. Specific Proteomic Identification of Collagen-Binding Proteins in Escherichia coli O157:H7: Characterisation of OmpA as a Potent Vaccine Antigen

Author

Monteiro, Ricardo, Chafsey, Ingrid, Caccia, Nelly, Ageorges, Valentin, Leroy, Sabine, Viala, Didier, Hébraud, Michel, Livrelli, Valérie, Pizza, Mariagrazia, Pezzicoli, Alfredo, Desvaux, Mickaël, Microbiologie Environnement Digestif Santé (MEDIS), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), GlaxoSmithKline [Siena, Italy] (GSK), Universidade do Porto = University of Porto, Plateforme Exploration du Métabolisme (PFEM), MetaboHUB-Clermont, MetaboHUB-MetaboHUB-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Imperial College London, ANR-17-CE21-0002,PathoFood,Décrypter l'hétérogénéité cellulaire et la distribution spatiale de pathogènes dans les matrices alimentaires en interaction avec les communautés microbiennes(2017), and European Project: 607611,EC:FP7:PEOPLE,FP7-PEOPLE-2013-ITN,DISCO(2014)

Subjects
collagen, host cell-bacteria interaction, [SDV]Life Sciences [q-bio], Escherichia coli, cell aggregation, intestinal epithelial cell, cell adhesion, cell surface protein, extracellular matrix (ECM), conserved antigens, diarrhoeagenic and intestinal bacterial pathogen
Abstract

International audience; Escherichia coli is a versatile commensal species of the animal gut that can also be a pathogen able to cause intestinal and extraintestinal infections. The plasticity of its genome has led to the evolution of pathogenic strains, which represent a threat to global health. Additionally, E. coli strains are major drivers of antibiotic resistance, highlighting the urgent need for new treatment and prevention measures. The antigenic and structural heterogeneity of enterohaemorrhagic E. coli colonisation factors has limited their use for the development of effective and cross-protective vaccines. However, the emergence of new strains that express virulence factors deriving from different E. coli diarrhoeagenic pathotypes suggests that a vaccine targeting conserved proteins could be a more effective approach. In this study, we conducted proteomics analysis and functional protein characterisation to identify a group of proteins potentially involved in the adhesion of E. coli O157:H7 to the extracellular matrix and intestinal epithelial cells. Among them, OmpA has been identified as a highly conserved and immunogenic antigen, playing a significant role in the adhesion phenotype of E. coli O157:H7 and in bacterial aggregation. Furthermore, antibodies raised against recombinant OmpA effectively reduced the adhesion of E. coli O157:H7 to intestinal epithelial cells. The present work highlights the role of OmpA as a potent antigen for the development of a vaccine against intestinal pathogenic E. coli.

Published
2023
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25. Expression of factor H binding protein in meningococcal strains can vary at least 15-fold and is genetically determined

Author

Biagini, Massimiliano, Spinsanti, Marco, De Angelis, Gabriella, Tomei, Sara, Ferlenghi, Ilaria, Scarselli, Maria, Rigat, Fabio, Messuti, Nicola, Biolchi, Alessia, Muzzi, Alessandro, Anderloni, Giulia, Brunelli, Brunella, Cartocci, Elena, Buricchi, Francesca, Tani, Chiara, Stella, Maria, Moschioni, Monica, Del Tordello, Elena, Colaprico, Annalisa, Savino, Silvana, Giuliani, Marzia M., Delany, Isabel, Pizza, Mariagrazia, Costantino, Paolo, Norais, Nathalie, Rappuoli, Rino, and Masignani, Vega

Published
2016

29. Structure of the meningococcal vaccine antigen NadA and epitope mapping of a bactericidal antibody

Author

Malito, Enrico, Biancucci, Marco, Faleri, Agnese, Ferlenghi, Ilaria, Scarselli, Maria, Maruggi, Giulietta, Surdo, Paola Lo, Veggi, Daniele, Liguori, Alessia, Santini, Laura, Bertoldi, Isabella, Petracca, Roberto, Marchi, Sara, Romagnoli, Giacomo, Cartocci, Elena, Vercellino, Irene, Savino, Silvana, Spraggon, Glen, Norais, Nathalie, Pizza, Mariagrazia, Rappuoli, Rino, Masignani, Vega, and Bottomley, Matthew James

Published
2014

33. Characterization of Enzyme-Linked Immunosorbent Assay (ELISA) for Quantification of Antibodies against Salmonella Typhimurium and Salmonella Enteritidis O-Antigens in Human Sera.

Author

Aruta, Maria Grazia, Lari, Elisa, De Simone, Daniele, Semplici, Bianca, Semplici, Claudia, Dale, Helen, Chirwa, Esmelda, Kadwala, Innocent, Mbewe, Maurice, Banda, Happy, Iturriza-Gomara, Miren, Gordon, Melita, Nyirenda, Tonney, Piu, Pietro, Pizza, Mariagrazia, Berlanda Scorza, Francesco, Grappi, Silvia, Canals, Rocío, and Rossi, Omar

Subjects
SALMONELLA typhimurium, ENZYME-linked immunosorbent assay, SALMONELLA enteritidis, IMMUNOGLOBULINS, VACCINE trials, VACCINE immunogenicity
Abstract

Nontyphoidal Salmonella (NTS) is a leading cause of morbidity and mortality caused by enteric pathogens worldwide in both children and adults, and vaccines are not yet available. The measurement of antigen-specific antibodies in the sera of vaccinated or convalescent individuals is crucial to understand the incidence of disease and the immunogenicity of vaccine candidates. A solid and standardized assay used to determine the level of specific anti-antigens IgG is therefore of paramount importance. In this work, we presented the characterization of a customized enzyme-linked immunosorbent assay (ELISA) with continuous readouts and a standardized definition of EU/mL. We assessed various performance parameters: standard curve accuracy, dilutional linearity, intermediate precision, specificity, limits of blanks, and quantification. The simplicity of the assay, its high sensitivity and specificity coupled with its low cost and the use of basic consumables and instruments without the need of high automation makes it suitable for transfer and application to different laboratories, including resource-limiting settings where the disease is endemic. This ELISA is, therefore, fit for purpose to be used for quantification of antibodies against Salmonella Typhimurium and Salmonella Enteritidis O-antigens in human samples, both for vaccine clinical trials and large sero-epidemiological studies. [ABSTRACT FROM AUTHOR]

Published
2023
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34. Predicted strain coverage of a meningococcal multicomponent vaccine (4CMenB) in Europe: a qualitative and quantitative assessment

Author

Vogel, Ulrich, Taha, Muhamed-Kheir, Vazquez, Julio A, Findlow, Jamie, Claus, Heike, Stefanelli, Paola, Caugant, Dominique A, Kriz, Paula, Abad, Raquel, Bambini, Stefania, Carannante, Anna, Deghmane, Ala Eddine, Fazio, Cecilia, Frosch, Matthias, Frosi, Giacomo, Gilchrist, Stefanie, Giuliani, Marzia M, Hong, Eva, Ledroit, Morgan, Lovaglio, Pietro G, Lucidarme, Jay, Musilek, Martin, Muzzi, Alessandro, Oksnes, Jan, Rigat, Fabio, Orlandi, Luca, Stella, Maria, Thompson, Danielle, Pizza, Mariagrazia, Rappuoli, Rino, Serruto, Davide, Comanducci, Maurizio, Boccadifuoco, Giuseppe, Donnelly, John J, Medini, Duccio, and Borrow, Ray

Published
2013
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35. Defining a protective epitope on factor H binding protein, a key meningococcal virulence factor and vaccine antigen

Author

Malito, Enrico, Faleri, Agnese, Surdo, Paola Lo, Veggi, Daniele, Maruggi, Giulietta, Grassi, Eva, Cartocci, Elena, Bertoldi, Isabella, Genovese, Alessia, Santini, Laura, Romagnoli, Giacomo, Borgogni, Erica, Brier, Sébastien, Passo, Carla Lo, Domina, Maria, Castellino, Flora, Felici, Franco, van der Veen, Stijn, Johnson, Steven, Lea, Susan M., Tang, Christoph M., Pizza, Mariagrazia, Savino, Silvana, Norais, Nathalie, Rappuoli, Rino, Bottomley, Matthew J., and Masignani, Vega

Published
2013

37. Identification by Reverse Vaccinology of Three Virulence Factors in Burkholderia cenocepacia That May Represent Ideal Vaccine Antigens.

Author

Irudal, Samuele, Scoffone, Viola Camilla, Trespidi, Gabriele, Barbieri, Giulia, D'Amato, Maura, Viglio, Simona, Pizza, Mariagrazia, Scarselli, Maria, Riccardi, Giovanna, and Buroni, Silvia

Subjects
BURKHOLDERIA cenocepacia, EXTRACELLULAR vesicles, BURKHOLDERIA cepacia, ANTIGENS, GREATER wax moth
Abstract

The Burkholderia cepacia complex comprises environmental and clinical Gram-negative bacteria that infect particularly debilitated people, such as those with cystic fibrosis. Their high level of antibiotic resistance makes empirical treatments often ineffective, increasing the risk of worst outcomes and the diffusion of multi-drug resistance. However, the discovery of new antibiotics is not trivial, so an alternative can be the use of vaccination. Here, the reverse vaccinology approach has been used to identify antigen candidates, obtaining a short-list of 24 proteins. The localization and different aspects of virulence were investigated for three of them—BCAL1524, BCAM0949, and BCAS0335. The three antigens were localized in the outer membrane vesicles confirming that they are surface exposed. We showed that BCAL1524, a collagen-like protein, promotes bacteria auto-aggregation and plays an important role in virulence, in the Galleria mellonella model. BCAM0949, an extracellular lipase, mediates piperacillin resistance, biofilm formation in Luria Bertani and artificial sputum medium, rhamnolipid production, and swimming motility; its predicted lipolytic activity was also experimentally confirmed. BCAS0335, a trimeric adhesin, promotes minocycline resistance, biofilm organization in LB, and virulence in G. mellonella. Their important role in virulence necessitates further investigations to shed light on the usefulness of these proteins as antigen candidates. [ABSTRACT FROM AUTHOR]

Published
2023
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38. Functional assays to evaluate antibody-mediated responses against Shigella: a review.

Author

Boero, Elena, Vezzani, Giacomo, Micoli, Francesca, Pizza, Mariagrazia, and Rossi, Omar

Subjects
IMMUNOGLOBULINS, SHIGELLA, SHIGELLOSIS, MIDDLE-income countries, BUILDING protection, INTRACELLULAR pathogens
Abstract

Shigella is a major global pathogen and the etiological agent of shigellosis, a diarrheal disease that primarily affects low- and middle-income countries. Shigellosis is characterized by a complex, multistep pathogenesis during which bacteria use multiple invasion proteins to manipulate and invade the intestinal epithelium. Antibodies, especially against the O-antigen and some invasion proteins, play a protective role as titres against specific antigens inversely correlate with disease severity; however, the context of antibody action during pathogenesis remains to be elucidated, especially with Shigella being mostly an intracellular pathogen. In the absence of a correlate of protection, functional assays rebuilding salient moments of Shigella pathogenesis can improve our understanding of the role of protective antibodies in blocking infection and disease. In vitro assays are important tools to build correlates of protection. Only recently animal models to recapitulate human pathogenesis, often not in full, have been established. This review aims to discuss in vitro assays to evaluate the functionality of anti-Shigella antibodies in polyclonal sera in light of the multistep and multifaced Shigella infection process. Indeed, measurement of antibody level alone may limit the evaluation of full vaccine potential. Serum bactericidal assay (SBA), and other functional assays such as opsonophagocytic killing assays (OPKA), and adhesion/invasion inhibition assays (AIA), are instead physiologically relevant and may provide important information regarding the role played by these effector mechanisms in protective immunity. Ultimately, the review aims at providing scientists in the field with new points of view regarding the significance of functional assays of choice which may be more representative of immunemediated protection mechanisms. [ABSTRACT FROM AUTHOR]

Published
2023
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42. Qualitative and quantitative assessment of meningococcal antigens to evaluate the potential strain coverage of protein-based vaccines

Author

Donnelly, John, Medini, Duccio, Boccadifuoco, Giuseppe, Biolchi, Alessia, Ward, Joel, Frasch, Carl, Moxon, E. Richard, Stella, Maria, Comanducci, Maurizio, Bambini, Stefania, Muzzi, Alessandro, Andrews, William, Chen, Jie, Santos, George, Santini, Laura, Boucher, Philip, Serruto, Davide, Pizza, Mariagrazia, Rappuoli, Rino, and Giuliani, Marzia Monica

Published
2010

43. Identification of protective and broadly conserved vaccine antigens from the genome of extraintestinal pathogenic Escherichia coli

Author

Moriel, Danilo Gomes, Bertoldi, Isabella, Spagnuolo, Angela, Marchi, Sara, Rosini, Roberto, Nesta, Barbara, Pastorello, Ilaria, Corea, Vanja A. Mariani, Torricelli, Giulia, Cartocci, Elena, Savino, Silvana, Scarselli, Maria, Dobrindt, Ulrich, Hacker, Jörg, Tettelin, Hervé, Tallon, Luke J., Sullivan, Steven, Wieler, Lothar H., Ewers, Christa, Pickard, Derek, Dougan, Gordon, Fontana, Maria Rita, Rappuoli, Rino, Pizza, Mariagrazia, and Serino, Laura

Published
2010

44. Neisseria Meningitidis GNA2132, a Heparin-Binding Protein That Induces Protective Immunity in Humans

Author

Serruto, Davide, Spadafina, Tiziana, Ciucchi, Laura, Lewis, Lisa A., Ram, Sanjay, Tontini, Marta, Santini, Laura, Biolchi, Alessia, Seib, Kate L., Giuliani, Marzia M., Donnelly, John J., Berti, Francesco, Savino, Silvana, Scarselli, Maria, Costantino, Paolo, Kroll, J. Simon, O'Dwyer, Clíona, Qiu, Jiazhou, Plaut, Andrew G., Moxon, Richard, Rappuoli, Rino, Pizza, Mariagrazia, and Aricò, Beatrice

Published
2010
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45. A Universal Vaccine for Serogroup B Meningococcus

Author

Giuliani, Marzia M., Adu-Bobie, Jeannette, Comanducci, Maurizio, Aricò, Beatrice, Savino, Silvana, Santini, Laura, Brunelli, Brunella, Bambini, Stefania, Biolchi, Alessia, Capecchi, Barbara, Cartocci, Elena, Ciucchi, Laura, Di Marcello, Federica, Ferlicca, Francesca, Galli, Barbara, Luzzi, Enrico, Masignani, Vega, Serruto, Davide, Veggi, Daniele, Contorni, Mario, Morandi, Maurizio, Bartalesi, Alessandro, Cinotti, Vanda, Mannucci, Donatella, Titta, Francesca, Ovidi, Elisa, Welsch, Jo Anne, Granoff, Dan, Rappuoli, Rino, and Pizza, Mariagrazia

Published
2006
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46. Vaccines for a sustainable planet.

Author

Pecetta, Simone, Nandi, Arindam, Weller, Charlie, Harris, Vanessa, Fletcher, Helen, Berlanda Scorza, Francesco, Pizza, Mariagrazia, Salisbury, David, Moxon, Richard, Black, Steve, Bloom, David E., and Rappuoli, Rino

Subjects
MICROBIAL diversity, DRUG resistance in microorganisms, VACCINES, SUSTAINABLE development, CLIMATE change
Abstract

The health of the planet is one objective of the United Nations' Sustainable Development Goals. Vaccines can affect not only human health but also planet health by reducing poverty, preserving microbial diversity, reducing antimicrobial resistance, and preventing an increase in pandemics that is fueled partly by climate change. [ABSTRACT FROM AUTHOR]

Published
2023
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47. Exploring the Role of GMMA Components in the Immunogenicity of a 4-Valent Vaccine against Shigella.

Author

Mancini, Francesca, Alfini, Renzo, Caradonna, Valentina, Monaci, Valentina, Carducci, Martina, Gasperini, Gianmarco, Piccioli, Diego, Biagini, Massimiliano, Giannelli, Carlo, Rossi, Omar, Pizza, Mariagrazia, and Micoli, Francesca

Subjects
VACCINE immunogenicity, SHIGELLA, IMMUNOGLOBULINS, DRUG resistance in microorganisms, HUMORAL immunity, TOLL-like receptors
Abstract

Shigellosis is the leading cause of diarrheal disease, especially in children of low- and middle-income countries, and is often associated with anti-microbial resistance. Currently, there are no licensed vaccines widely available against Shigella, but several candidates based on the O-antigen (OAg) portion of lipopolysaccharides are in development. We have proposed Generalized Modules for Membrane Antigens (GMMA) as an innovative delivery system for OAg, and a quadrivalent vaccine candidate containing GMMA from S. sonnei and three prevalent S. flexneri serotypes (1b, 2a and 3a) is moving to a phase II clinical trial, with the aim to elicit broad protection against Shigella. GMMA are able to induce anti-OAg-specific functional IgG responses in animal models and healthy adults. We have previously demonstrated that antibodies against protein antigens are also generated upon immunization with S. sonnei GMMA. In this work, we show that a quadrivalent Shigella GMMA-based vaccine is able to promote a humoral response against OAg and proteins of all GMMA types contained in the investigational vaccine. Proteins contained in GMMA provide T cell help as GMMA elicit a stronger anti-OAg IgG response in wild type than in T cell-deficient mice. Additionally, we observed that only the trigger of Toll-like Receptor (TLR) 4 and not of TLR2 contributed to GMMA immunogenicity. In conclusion, when tested in mice, GMMA of a quadrivalent Shigella vaccine candidate combine both adjuvant and carrier activities which allow an increase in the low immunogenic properties of carbohydrate antigens. [ABSTRACT FROM AUTHOR]

Published
2023
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49. Effectiveness of meningococcal B vaccine against endemic hypervirulent Neisseria meningitidis W strain, England

Author

Ladhani, Shamez N., Giuliani, Marzia Monica, Biolchi, Alessia, Pizza, Mariagrazia, Beebeejaun, Kazim, Lucidarme, Jay, Findlow, Jamie, Ramsay, Mary E., and Borrow, Ray

Subjects
Meningococcal infections -- Prevention, Bacterial vaccines -- Testing, Health
Abstract

Invasive meningococcal disease (IMD) has been declining in the United Kingdom since the early 2000s (1). Historically, serogroup W Neisseria meningitides meningococci (MenW) have been causal organisms for 1%-2% of [...]

Published
2016
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