16 results on '"Pinto, Susana C."'
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2. Boosting in vitro cartilage tissue engineering through the fabrication of polycaprolactone-gelatin 3D scaffolds with specific depth-dependent fiber alignments and mechanical stimulation
- Author
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Semitela, Ângela, Girão, André F., Fernandes, Carla, Ramalho, Gonçalo, Pinto, Susana C., Completo, António, and Marques, Paula A.A.P.
- Published
- 2021
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3. Multifunctional hybrid structures made of open-cell aluminum foam impregnated with cellulose/graphene nanocomposites
- Author
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Pinto, Susana C., Silva, Nuno H.C.S., Pinto, Ricardo J.B., Freire, Carmen S.R., Duarte, Isabel, Vicente, Romeu, Vesenjak, Matej, and Marques, Paula A.A.P.
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- 2020
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4. Bacterial cellulose/graphene oxide aerogels with enhanced dimensional and thermal stability
- Author
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Pinto, Susana C., Gonçalves, Gil, Sandoval, Stefania, López-Periago, Ana M., Borras, Alejandro, Domingo, Concepción, Tobias, Gerard, Duarte, Isabel, Vicente, Romeu, and Marques, Paula A.A.P.
- Published
- 2020
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5. Characterization and physical properties of aluminium foam–polydimethylsiloxane nanocomposite hybrid structures
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Pinto, Susana C., Marques, Paula A.A.P., Vesenjak, Matej, Vicente, Romeu, Godinho, Luís, Krstulović-Opara, Lovre, and Duarte, Isabel
- Published
- 2019
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6. Development and characterization of aluminum alloy foam - cork hybrid structures
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Sousa, João D. P., Pinto, Susana C., Vesenjak, Matej, Carneiro, Vitor H., Krstulović-Opara, Lovre, Marques, Paula A. P., and Duarte, Isabel
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Hybrid structures ,Manufacturing ,Characterization ,Aluminium alloy foam ,Cork - Abstract
Cellular solids and porous materials have been considered as one of the most suitable lightweight multifunctional materials for a wide range of commercial and industrial applications, e.g. in medicine, military. Their use contributes to an immediate and significant weight reduction and material savings of the components but also to multifunctionality due to their 3D cellular structures (open-cells or closedcells). Herein, hybrid structures based on cellular materials are developed and studied by combining open-cell aluminum foam with cork. These hybrid structures were prepared by infiltrating a mixture containing polymer-coated cork powders into the open-cell foam. The samples are geometrically analyzed using X-ray microcomputed tomography to extract morphological and topological properties of the voids and the solid phase. The mechanical, thermal, acoustic, and fire retardancy properties of these aluminum foam-cork hybrid structures are evaluated and compared with their individual components (open-cell aluminum alloy foam and agglomerated cork). published
- Published
- 2022
7. Multiscale Sensing of Bone-Implant Loosening for Multifunctional Smart Bone Implants: Using Capacitive Technologies for Precision Controllability.
- Author
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Peres, Inês, Rolo, Pedro, Ferreira, Jorge A. F., Pinto, Susana C., Marques, Paula A. A. P., Ramos, António, and Soares dos Santos, Marco P.
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CAPACITIVE sensors ,SENSOR networks ,RASPBERRY Pi ,INTERNET servers ,REOPERATION ,WEB hosting ,ARTIFICIAL joints - Abstract
The world population growth and average life expectancy rise have increased the number of people suffering from non-communicable diseases, namely osteoarthritis, a disorder that causes a significant increase in the years lived with disability. Many people who suffer from osteoarthritis undergo replacement surgery. Despite the relatively high success rate, around 10% of patients require revision surgeries, mostly because existing implant technologies lack sensing devices capable of monitoring the bone–implant interface. Among the several monitoring methodologies already proposed as substitutes for traditional imaging methods, cosurface capacitive sensing systems hold the potential to monitor the bone–implant fixation states, a mandatory capability for long-term implant survival. A multifaceted study is offered here, which covers research on the following points: (1) the ability of a cosurface capacitor network to effectively monitor bone loosening in extended peri-implant regions and according to different stimulation frequencies; (2) the ability of these capacitive architectures to provide effective sensing in interfaces with hydroxyapatite-based layers; (3) the ability to control the operation of cosurface capacitive networks using extracorporeal informatic systems. In vitro tests were performed using a web-based network sensor composed of striped and interdigitated capacitive sensors. Hydroxyapatite-based layers have a minor effect on determining the fixation states; the effective operation of a sensor network-based solution communicating through a web server hosted on Raspberry Pi was shown. Previous studies highlight the inability of current bone–implant fixation monitoring methods to significantly reduce the number of revision surgeries, as well as promising results of capacitive sensing systems to monitor micro-scale and macro-scale bone–interface states. In this study, we found that extracorporeal informatic systems enable continuous patient monitoring using cosurface capacitive networks with or without hydroxyapatite-based layers. Findings presented here represent significant advancements toward the design of future multifunctional smart implants. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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8. Association of NIPA1 repeat expansions with amyotrophic lateral sclerosis in a large international cohort
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Tazelaar, Gij SHP, Dekker, Annelot M, van Vugt, Joke JFA, van der Spek, Rick A, Westeneng, Henk-Jan, Kool, Lindy JBG, Kenna, Kevin P, van Rheenen, Wouter, Pulit, Sara L, McLaughlin, Russell L, Sproviero, William, Iacoangeli, Alfredo, Huebers, Annemarie, Brenner, David, Morrison, Karen E, Shaw, Pamela J, Shaw, Christopher E, Povedano Panades, Monica, Mora Pardina, Jesus S, Glass, Jonathan D, Hardiman, Orla, Al-Chalabi, Ammar, van Damme, Philip, Robberecht, Wim, Landers, John E, Ludolph, Albert C, Weishaupt, Jochen H, van den Berg, Leonard H, Veldink, Jan H, van Es, Michael A, Akcimen, Fulya, Al Khleifat, Ahmad, Andersen, Peter, Basak, A Nazli, Bauer, Denis C, Blair, Ian, Brands, William J, Byrne, Ross P, Calvo, Andrea, Gonzalez, Yolanda Campos, Chio, Adriano, Cooper-Knock, Jonothan, Corcia, Philippe, Couratier, Philippe, de Carvalho, Mamede, Drory, Vivian E, Eitan, Chen, Garcia Redondo, Alberto, Gellera, Cinzia, Gotkine, Marc, Hornstein, Eran, Kenna, Brendan, Kiernan, Matthew C, Kocoglu, Cemile, Kooyman, Maarten, Lopez Alonso, Victoria, Middelkoop, Bas, Mill, Jonathan, Mitne-Neto, Miguel, Moisse, Matthieu, Pinto, Susana C, Ratti, Antonia, Schellevis, Raymond D, Shatunov, Aleksey, Silani, Vincenzo, Staiger, Christine, Tazelaar, Gijs HP, Ticozzi, Nicola, Tunca, Ceren, Twine, Nathalie A, van Doormaal, Perry TC, van Eijk, Kristel R, Visscher, Peter M, Vourch, Patrick, Weber, Markus, Williams, Kelly L, Wray, Naomi, Yang, Jian, Zatz, Mayana, and Zhang, Katharine
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0301 basic medicine ,Oncology ,Male ,Aging ,Geriatrics & Gerontology ,Internationality ,Cohort Studies ,0302 clinical medicine ,Copy-number variation ,Amyotrophic lateral sclerosis ,Non-U.S. Gov't ,DNA Repeat Expansion ,General Neuroscience ,Research Support, Non-U.S. Gov't ,Amyotrophic Lateral Sclerosis/genetics ,GENOME ,Cohort ,Female ,Life Sciences & Biomedicine ,Cohort study ,medicine.medical_specialty ,Hereditary spastic paraplegia ,Neuroscience(all) ,NIPA1 ,Clinical Neurology ,HEREDITARY SPASTIC PARAPLEGIA ,Research Support ,Article ,03 medical and health sciences ,DNA Repeat Expansion/genetics ,Meta-Analysis as Topic ,Internal medicine ,Angelman syndrome ,medicine ,Journal Article ,Humans ,Membrane Proteins/genetics ,Genetic Association Studies ,Science & Technology ,business.industry ,Neurosciences ,Membrane Proteins ,Repeat expansion ,medicine.disease ,Ageing ,030104 developmental biology ,Logistic Models ,Neurosciences & Neurology ,Neurology (clinical) ,Geriatrics and Gerontology ,Peptides ,business ,Trinucleotide repeat expansion ,Peptides/genetics ,030217 neurology & neurosurgery ,Developmental Biology - Abstract
NIPA1 (nonimprinted in Prader-Willi/Angelman syndrome 1) mutations are known to cause hereditary spastic paraplegia type 6, a neurodegenerative disease that phenotypically overlaps to some extent with amyotrophic lateral sclerosis (ALS). Previously, a genomewide screen for copy number variants found an association with rare deletions in NIPA1 and ALS, and subsequent genetic analyses revealed that long (or expanded) polyalanine repeats in NIPA1 convey increased ALS susceptibility. We set out to perform a large-scale replication study to further investigate the role of NIPA1 polyalanine expansions with ALS, in which we characterized NIPA1 repeat size in an independent international cohort of 3955 patients with ALS and 2276 unaffected controls and combined our results with previous reports. Meta-analysis on a total of 6245 patients with ALS and 5051 controls showed an overall increased risk of ALS in those with expanded (>8) GCG repeat length (odds ratio = 1.50, p = 3.8×10-5). Together with previous reports, these findings provide evidence for an association of an expanded polyalanine repeat in NIPA1 and ALS. ispartof: NEUROBIOLOGY OF AGING vol:74 ispartof: location:United States status: published
- Published
- 2019
9. Multifuncional hybrid foams composed by aluminium open-cell foam filled with polymers
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Pinto, Susana C., Marques, Paula A.A.P., Vesenjak, Matej, Vicente, Romeu, Krstulović-Opara, Lovre, Duarte, Isabel, Duarte, Isabel, Vesenjak, Matej, and Ren, Zoran
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open-cell aluminium foam ,polydimenthylsiloxane ,hybrid foam ,graphene-based materials ,compressive behaviour - Abstract
Multifunctional materials represent one of the most current and promising class of materials for engineering applications. This type of materials shows enhanced performance resulting from the combination of the individual constituents’ properties [1]. Hybrid foams are a particular class of these materials usually prepared by filling the voids of the foam with a secondary material. Aluminum open-cell foams are one of the most interesting multifunctional materials with applicable properties, such as high thermal and electrical conductivities and high internal surface area, recyclability and non-flammability. However, they are mechanically weak compared to other materials. This drawback can be overcome with the combination of a stronger filling material (polymer) [2, 3]. In this study, an aluminum open-cell (OC) foam was filled with polydimethylsiloxane (PDMS), resulting in polydimethylsiloxane-aluminum (PDMS-OC) hybrid foam. Quasi-static and dynamic uniaxial compressive tests and infrared thermography were performed to compare the PDMS-OC hybrid foams with the individual components (OC foam and PDMS). The effect of the incorporation at a low content of graphene-based materials (GBMs) dispersed into the PDMS matrix was also evaluated. Results show an improvement of the compressive strength and energy absorption capacity of hybrid foams compared to the individual components (OC foams and PDMS). The use of the PDMS as a void filler also changed the typical layer-wise collapse mechanism of the aluminum open-cell foam. Simultaneously, the elastomeric behavior of PDMS allowed that the material can undergo higher loads without cracking. The PDMS enforced a symmetric deformation by folding in the middle of the hybrid foams. The high energy absorption values of aluminum OC foams embedded with PDMS compensated for the mass increase due to the PDMS filler. The incorporation of GBMs introduces voids into the PDMS matrix and avoids higher degree of crosslinking/polymerization in PDMS. The thermal conductivity of the OC/PDMS hybrid foams was slightly higher comparatively to the PDMS specimens. This effect is more pronounced with the addition of GBMs in the PDMS matrix.
- Published
- 2019
10. Catalytic activity of trypsin entrapped in electrospun poly(ϵ-caprolactone) nanofibers.
- Author
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Pinto, Susana C., Rodrigues, Ana R., Saraiva, Jorge A., and Lopes-da-Silva, José A.
- Subjects
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TRYPSIN , *CAPROLACTONES , *NANOFIBERS , *EMULSIONS , *DIMETHYLFORMAMIDE , *CATALYTIC activity , *MOLECULAR weights , *ELECTROSPINNING - Abstract
Trypsin was successfully entrapped in situ into nanofibers of poly(ϵ-caprolactone) (PCL) prepared by electrospinning. The spinning dope was an emulsion consisting of an aqueous phase with the solubilized enzyme in a pH buffer plus an oil phase of the polymer solubilized in chloroform (CF)/dimethylformamide (DMF). The optimized materials were composed by random arrays of bead-free fibers with outer diameters in the range 110–180 nm without showing core–shell structure. The fiber size and morphology, membrane porosity and surface properties were shown to be influenced by the polymer concentration and the composition ratio of the solvent mixture, and also by the presence of the enzyme. The activity of the immobilized trypsin was studied toward both a low-molecular weight synthetic substrate (BAPNA) and a protein (casein). Fluorescence microscopy, the increasing hydrophilicity of the fibrous membrane and the observed catalytic activity confirmed the entrapment of the enzyme into the PCL nanofibers. The best activity retention (∼66% toward BAPNA) was achieved using 0.20 g/mL PCL in CF/DMF [75:25], with trypsin in an aqueous buffer at pH 7.1 in the presence of benzamidine and Span80. The immobilized enzyme showed satisfactory operational stability retaining ∼59% of its initial activity after five reaction cycles. Compared with the free enzyme, the storage (at 4 °C) and thermal stability of the immobilized enzyme were highly improved. The retained catalytic activity and the observed reusability can be explained by a heterogeneous distribution of the enzyme within the polymer fiber influenced by the electrostatic field during the electrospinning process, enabling a preferential location near the fiber surface but simultaneously assuring minimal leaching out during operations. Results suggest that trypsin-PCL fibrous membranes may be useful for concomitant proteolytic and separation commercial applications. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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11. Hybrid Structures Made of Polyurethane/Graphene Nanocomposite Foams Embedded within Aluminum Open-Cell Foam.
- Author
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Pinto, Susana C., Marques, Paula A. A. P., Vicente, Romeu, Godinho, Luís, and Duarte, Isabel
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CARBON foams ,ALUMINUM foam ,POROUS materials ,FOAM ,NANOCOMPOSITE materials ,URETHANE foam - Abstract
This paper focuses on the development of hybrid structures containing two different classes of porous materials, nanocomposite foams made of polyurethane combined with graphene-based materials, and aluminum open-cell foams (Al-OC). Prior to the hybrid structures preparation, the nanocomposite foam formulation was optimized. The optimization consisted of studying the effect of the addition of graphene oxide (GO) and graphene nanoplatelets (GNPs) at different loadings (1.0, 2.5 and 5.0 wt%) during the polyurethane foam (PUF) formation, and their effect on the final nanocomposite properties. Globally, the results showed enhanced mechanical, acoustic and fire-retardant properties of the PUF nanocomposites when compared with pristine PUF. In a later step, the hybrid structure was prepared by embedding the Al-OC foam with the optimized nanocomposite formulation (prepared with 2.5 wt% of GNPs (PUF/GNPs2.5)). The process of filling the pores of the Al-OC was successfully achieved, with the resulting hybrid structure retaining low thermal conductivity values, around 0.038 W∙m
−1 ∙K−1 , and presenting an improved sound absorption coefficient, especially for mid to high frequencies, with respect to the individual foams. Furthermore, the new hybrid structure also displayed better mechanical properties (the stress corresponding to 10% of deformation was improved in more than 10 and 1.3 times comparatively to PUF/GNPs2.5 and Al-OC, respectively). [ABSTRACT FROM AUTHOR]- Published
- 2020
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12. Mechanical, Thermal, and Acoustic Properties of Aluminum Foams Impregnated with Epoxy/Graphene Oxide Nanocomposites.
- Author
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Pinto, Susana C., Marques, Paula A.A.P., Vesenjak, Matej, Vicente, Romeu, Godinho, Luís, Krstulović-Opara, Lovre, and Duarte, Isabel
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GRAPHENE oxide ,ALUMINUM foam ,ABSORPTION of sound ,ABSORPTION coefficients ,COMPRESSIVE strength ,THERMAL stability ,EPOXY resins - Abstract
Hybrid structures with epoxy embedded in open-cell aluminum foam were developed by combining open-cell aluminum foam specimens with unreinforced and reinforced epoxy resin using graphene oxide. These new hybrid structures were fabricated by infiltrating an open-cell aluminum foam specimen with pure epoxy or mixtures of epoxy and graphene oxide, completely filling the pores. The effects of graphene oxide on the mechanical, thermal, and acoustic performance of epoxy/graphene oxide-based nanocomposites are reported. Mechanical compression analysis was conducted through quasi-static uniaxial compression tests at two loading rates (0.1 mm/s and 1 mm/s). Results show that the thermal stability and the sound absorption coefficient of the hybrid structures were improved by the incorporation of the graphene oxide within the epoxy matrix. However, the incorporation of the graphene oxide into the epoxy matrix can create voids inside the epoxy resin, leading to a decrease of the compressive strength of the hybrid structures, thus no significant increase in the energy absorption capability was observed. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
13. Graphene-Enriched Agglomerated Cork Material and Its Behaviour under Quasi-Static and Dynamic Loading.
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Ptak, Mariusz, Kaczyński, Paweł, Wilhelm, Johannes, Margarido, José M. T., Marques, Paula A. A. P., Pinto, Susana C., Alves de Sousa, Ricardo J., and Fernandes, Fábio A. O.
- Subjects
GRAPHENE oxide ,QUASIPARTICLES ,NANOSTRUCTURED materials ,NANOPARTICLES ,CORK oak - Abstract
The use of cork for a variety of applications has been gaining significance due to environmental concerns and political agendas. Consequently, its range of applications is growing rapidly. In this work, aiming to improve its mechanical response for crashworthiness applications, cork agglomerates were enriched by small quantities of graphene oxide or graphene nanoplates in order to observe a resulting improvement of the mechanical behaviour during quasi-static and dynamic compressive loading cases. To produce homogenous cork agglomerates including graphene, the material was previously dispersed into granulated cork using stirrers to achieve a good distribution. Then, the typical procedure of compression and curing was carried out. Magnified images attest a good dispersion of graphene into the cork matrix. Mechanical testing was performed for a variety of graphene concentrations (0.1, 0.5 and 1.0 weight %), becoming clear that the beneficial effect of including graphene (either oxide or nanoplates) is related to a later densification stage while keeping the same stress plateau levels. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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14. Fabrication of Customizable and Reproducible 3D Chondrocyte-Laden Nanofibrous Architectures: Effect of Specific Fiber Alignments and Porosities on Chondrocyte Response under Cyclic Compression.
- Author
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Semitela Â, Pinto SC, Capitão A, Marques PAAP, and Completo A
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- Tissue Scaffolds chemistry, Porosity, Water, Chondrocytes, Nanofibers chemistry
- Abstract
Electrospinning has been widely employed to fabricate complex extracellular matrix-like microenvironments for tissue engineering due to its ability to replicate structurally biomimetic micro- and nanotopographic cues. Nevertheless, these nanofibrous structures are typically either confined to bidimensional systems or confined to three-dimensional ones that are unable to provide controlled multiscale patterns. Thus, an electrospinning modality was used in this work to fabricate chondrocyte-laden nanofibrous scaffolds with highly customizable three-dimensional (3D) architectures in an automated manner, with the ultimate goal of recreating a suitable 3D scaffold for articular cartilage tissue engineering. Three distinct architectures were designed and fabricated by combining multiple nanofibrous and chondrocyte-laden hydrogel layers and tested in vitro in a compression bioreactor system. Results demonstrated that it was possible to precisely control the placement and alignment of electrospun polycaprolactone and gelatin nanofibers, generating three unique architectures with distinctive macroscale porosity, water absorption capacity, and mechanical properties. The architecture organized in a lattice-like fashion was highly porous with substantial pore interconnectivity, resulting in a high-water absorption capacity but a poor compression modulus and relatively weaker energy dissipation capacity. The donut-like 3D geometry was the densest, with lower swelling, but the highest compression modulus and improved energy dissipation ability. The third architecture combined a lattice and donut-like fibrous arrangement, exhibiting intermediary behavior in terms of porosity, water absorption, compression modulus, and energy dissipation capacity. The properties of the donut-like 3D architecture demonstrated great potential for articular cartilage tissue engineering, as it mimicked key topographic, chemical, and mechanical characteristics of chondrocytes' surrounding environment. In fact, the combination of these architectural features with a dynamically compressive mechanical stimulus triggered the best in vitro results in terms of viability and biosynthetic production.
- Published
- 2023
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15. Interfacing reduced graphene oxide with an adipose-derived extracellular matrix as a regulating milieu for neural tissue engineering.
- Author
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Barroca N, da Silva DM, Pinto SC, Sousa JPM, Verstappen K, Klymov A, Fernández-San-Argimiro FJ, Madarieta I, Murua O, Olalde B, Papadimitriou L, Karali K, Mylonaki K, Stratakis E, Ranella A, and Marques PAAP
- Subjects
- Neurons, Extracellular Matrix chemistry, Tissue Engineering, Graphite chemistry
- Abstract
Enthralling evidence of the potential of graphene-based materials for neural tissue engineering is motivating the development of scaffolds using various structures related to graphene such as graphene oxide (GO) or its reduced form. Here, we investigated a strategy based on reduced graphene oxide (rGO) combined with a decellularized extracellular matrix from adipose tissue (adECM), which is still unexplored for neural repair and regeneration. Scaffolds containing up to 50 wt% rGO relative to adECM were prepared by thermally induced phase separation assisted by carbodiimide (EDC) crosslinking. Using partially reduced GO enables fine-tuning of the structural interaction between rGO and adECM. As the concentration of rGO increased, non-covalent bonding gradually prevailed over EDC-induced covalent conjugation with the adECM. Edge-to-edge aggregation of rGO favours adECM to act as a biomolecular physical crosslinker to rGO, leading to the softening of the scaffolds. The unique biochemistry of adECM allows neural stem cells to adhere and grow. Importantly, high rGO concentrations directly control cell fate by inducing the differentiation of both NE-4C cells and embryonic neural progenitor cells into neurons. Furthermore, primary astrocyte fate is also modulated as increasing rGO boosts the expression of reactivity markers while unaltering the expression of scar-forming ones., (Copyright © 2023. Published by Elsevier B.V.)
- Published
- 2023
- Full Text
- View/download PDF
16. Ultraviolet Functionalization of Electrospun Scaffolds to Activate Fibrous Runways for Targeting Cell Adhesion.
- Author
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Girão AF, Wieringa P, Pinto SC, Marques PAAP, Micera S, van Wezel R, Ahmed M, Truckenmueller R, and Moroni L
- Abstract
A critical challenge in scaffold design for tissue engineering is recapitulating the complex biochemical patterns that regulate cell behavior in vivo . In this work, we report the adaptation of a standard sterilization methodology-UV irradiation-for patterning the surfaces of two complementary polymeric electrospun scaffolds with oxygen cues able to efficiently immobilize biomolecules. Independently of the different polymer chain length of poly(ethylene oxide terephthalate)/poly(butylene terephthalate) (PEOT/PBT) copolymers and PEOT/PBT ratio, it was possible to easily functionalize specific regions of the scaffolds by inducing an optimized and spatially controlled adsorption of proteins capable of boosting the adhesion and spreading of cells along the activated fibrous runways. By allowing an efficient design of cell attachment patterns without inducing any noticeable change on cell morphology nor on the integrity of the electrospun fibers, this procedure offers an affordable and resourceful approach to generate complex biochemical patterns that can decisively complement the functionality of the next generation of tissue engineering scaffolds.
- Published
- 2019
- Full Text
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