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658 results on '"Patil, Rahul A."'

28. Single-Dose Drug Development Candidate for Schistosomiasis.

Author

Leas, Derek A., Keiser, Jennifer, Charman, Susan A., Shackleford, David M., Jones, Jeremy O., Campbell, Michael, Chen, Gong, Katneni, Kasiram, Patil, Rahul, Hu, Meiyu, Pham, Thao, Häberli, Cécile, Schulze, Thomas T., Neville, Andrew J., Wang, Xiaofang, Dong, Yuxiang, Davis, Paul H., and Vennerstrom, Jonathan L.

Published
2024
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34. Changing pattern of admissions for acute myocardial infarction in India during the COVID-19 pandemic

Author

Zachariah, Geevar, Ramakrishnan, Sivasubramanian, Das, Mrinal Kanti, Jabir, Abdullakutty, Jayagopal, Pathiyil Balagopalan, Venugopal, Krishnannair, Mani, Kalaivani, Khan, Amal Kumar, Malviya, Amit, Gupta, Anshul, Goyal, Ashok, Singh, B.P., Mohan, Bishav, Bharti, Bishwa Bhushan, Majumder, Biswajit, Wilson, Bivin, Karunadas, Chakkalakkal Prabhakaran, Meena, Chandra Bhan, Manjunath, Cholenahally Nanjappa, Cibu, Mathew, Roy, Debabrata, Choudhary, Dinesh, Das, Dipak Ranjan, Sarma, Dipak, Girish, Meennahalli Palleda, Wander, Gurpreet Singh, Wardhan, Harsh, Ezhilan, Janakiraman, Tummala, Karthik, Katyal, Virender Kumar, Goswami, Kewal, Subramanyam, Kodangala, Goyal, Krishna Kishore, Kumar, Kenchappa, Pathak, Lekha Adik, Bansal, Manish, Mandal, Manoranjan, Gupta, Mohit Dayal, Khanna, Narendra Nath, Hanumanthappa, Natesh Bangalore, Bardoloi, Neil, Modi, Nitin, Naik, Nitish, Hasija, Pradeep K., Kerkar, Prafulla, Bhattacharyya, Pranab Jyoti, Gadkari, Pushkraj, Chakraborthy, Rabindra Nath, Patil, Rahul Raosaheb, Gupta, Rakesh, Yadav, Rakesh, Murty, Rambhatla Suryanarayana, Nath, Ranjit Kumar, Sivakumar, Rathinavel, Sethi, Rishi, Baruah, Rituparna, Tyagi, Sanjay, Guha, Santanu, Krishnappa, Santhosh, Kumar, Satish, Routray, Satya Narayan, Tewari, Satyendra, Ray, Saumitra, Reddy, Seemala Saikrishna, Chandra, Sharad, Gupta, Shashi Bhushan, Chatterjee, Shashi Shekhar, Siddiqui, K.K.H., Sivabalan, Maduramuthu, Yerram, Sreekanth, Kumar, Sudeep, Nagarajan, Swaminathan, Devasia, Tom, Jadhav, Uday, Narain, Varun Shankar, Garg, Vijay Kumar, Gupta, Vitull K., Prabhakaran, Dorairaj, Deb, Pradip Kumar, and Mohanan, Padinhare Purayil

Published
2021
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45. Extracellular purines in lung endothelial permeability and pulmonary diseases.

Author

Gerasimovskaya, Evgenia, Patil, Rahul S., Davies, Adrian, Maloney, McKenzie E., Simon, Liselle, Mohamed, Basmah, Cherian-Shaw, Mary, and Verin, Alexander D.

Subjects
ADENINE nucleotides, ENDOTHELIAL cells, VASCULAR endothelium, PURINERGIC receptors, LUNG diseases
Abstract

The purinergic signaling system is an evolutionarily conserved and critical regulatory circuit that maintains homeostatic balance across various organ systems and cell types by providing compensatory responses to diverse pathologies. Despite cardiovascular diseases taking a leading position in human morbidity and mortality worldwide, pulmonary diseases represent significant health concerns as well. The endothelium of both pulmonary and systemic circulation (bronchial vessels) plays a pivotal role in maintaining lung tissue homeostasis by providing an active barrier and modulating adhesion and infiltration of inflammatory cells. However, investigations into purinergic regulation of lung endothelium have remained limited, despite widespread recognition of the role of extracellular nucleotides and adenosine in hypoxic, inflammatory, and immune responses within the pulmonary microenvironment. In this review, we provide an overview of the basic aspects of purinergic signaling in vascular endothelium and highlight recent studies focusing on pulmonary microvascular endothelial cells and endothelial cells from the pulmonary artery vasa vasorum. Through this compilation of research findings, we aim to shed light on the emerging insights into the purinergic modulation of pulmonary endothelial function and its implications for lung health and disease. [ABSTRACT FROM AUTHOR]

Published
2024
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46. Improved methods for total and chloroplast protein extraction from Cajanus species for two-dimensional gel electrophoresis and mass spectrometry.

Author

S., Arunima, Das, Alakesh, Kalita, Prakash Jyoti, Patil, Rahul Ishwar, Pandey, Neha, Bhattacharjee, Mamta, Sharma, Bidyut Kumar, Das, Debajit, and Acharjee, Sumita

Subjects
LIQUID chromatography-mass spectrometry, PIGEON pea, CHLOROPLAST DNA, PEA proteins, TWO-dimensional electrophoresis
Abstract

The recent advances in pigeon pea genomics, including high-quality whole genome and chloroplast genome sequence information helped develop improved varieties. However, a comprehensive Cajanus proteome, including the organelle proteome, is yet to be fully mapped. The spatial delineation of pigeon pea proteins at sub-cellular levels and inter-organelle communication could offer valuable insights into its defense mechanism against various stresses. However, the major bottleneck in the proteomic study is the lack of a suitable method of protein extraction and sample preparation compatible with two-dimensional gel electrophoresis (2D-PAGE), liquid chromatography-mass spectrometry (LCMS), or matrix-assisted laser desorption ionization-time of flight (MALDi-ToF). Our study introduces two efficient methods, one for isolating total proteins and another for organelle (chloroplast) proteins from various Cajanus spp. For total protein extraction, we have optimized a protocol using phenol in combination with a reducing agent (DTT) and protease inhibitor cocktail, also washing (6–7 times) with ice-cold acetone after overnight protein precipitation of total proteins. Our modified extraction method using phenol for total leaf protein yielded approximately 2-fold more proteins than the previously reported protocols from C. cajan (3.18 ± 0.11 mg/gm) and C. scarabaeoides (2.06 ± 0.08 mg/gm). We have also optimized a protocol for plastid protein extraction, which yielded 1.33 ± 0.25 mg/10 gm plastid proteins from C. cajan and 0.88 ± 0.19 mg/10 gm plastid proteins from C. scarabaeoides. The 2D-PAGE analysis revealed 678 ± 08 reproducible total protein spots from C. cajan and 597 ± 22 protein spots from C. scarabaeoides. Similarly, we found 566 ± 10 and 486 ± 14 reproducible chloroplast protein spots in C. cajan and C. scarabaeoides, respectively. We confirmed the plastid protein fractions through immunoblot analysis using antibodies against LHCb1/LHCⅡ type Ⅰ protein. We found both methods suitable for 2D-PAGE and mass spectrometry (MS). This is the first report on developing protocols for total and chloroplastic protein extraction of Cajanus spp. suitable for advanced proteomics research. [ABSTRACT FROM AUTHOR]

Published
2024
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47. Improving FFMPEG Performance with RTMP Server Using SDNControlled Routing in Containerized Wireless Mesh Networks.

Author

Kadam, Sanjay, Pawar, Vaibhav, Sale, Hanamant, Patil, Rahul, Gund, Ankush, Patel, Pravinkumar, and Jadhav, Mahadeo

Subjects
COMPUTER network traffic, STREAMING video & television, SOFTWARE-defined networking, COMPUTER network security, INTERNET service providers, WIRELESS mesh networks
Abstract

Because traditional networks lack flexibility, agility, and scalability, the highly demanding Over-The-Top (OTT) multimedia apps pose additional challenges for Internet Service Providers (ISPs) in providing a good Quality of Experience (QoE) to their customers. Through the use of software-defined networks (SDN) and network function virtualization, future networks will cloudify their network resources (NFV). New technologies, such as softwaredefined networking and network function virtualization, play a very important role in modernizing wireless mesh networks and improving bandwidth, latency, packet drop, and network security. In this paper we compared the multipath routing and forward routing. In this architecture we used unicast transmission through multipath packet forwarding using the POX controller and Forward routing sends network traffic from a source node to a destination node over a single route. This technique defines the route statically or dynamically depending on network factors such connection availability, congestion, and latency. Even if there are numerous paths in the network, all multipath packet forwarding is restricted to one path by using this controller. Real-time Message Protocol server for internet video networks: FFmpeg. Video streaming allows encoding, transcoding, effects, and more. FFmpeg simplifies live stream production and other video operations. FFmpeg streams RTMP, the early internet video streaming protocol. TCP-based RTMP provides persistent, low-latency streaming. This study proposes streaming the video of a virtualized wireless host container to wired and wireless clients using an FFmpeg RTMP server address outside the wireless mesh architecture and assessing its controller performance by collecting data from wired and wireless hosts. The container in the wireless mesh topology and outside topology may transmit video. [ABSTRACT FROM AUTHOR]

Published
2024

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