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The study was carried out in three selected metro areas of Chattogram city of Bangladesh covering 90 sample households. Proportionate random sampling technique was followed. Results revealed that the average rooftop space per household was recorded as 2248.23 sq. feet; whereas 1268.4 sq. feet (56.42%) was under rooftop garden (RTG), 588.5 sq. feet (26.17%) was remained as open and 391.3 sq. feet (17.41%) was identified as potential space for expanding the garden. All of the gardens were installed at 2-10th stories of the buildings. As crop diversity 26 types of vegetables, 39 types of fruits, 11 types spices and 22 types of flower/ornamental and medicinal plants were found to be grown in the current RTG’s. But the number of crops varies significantly among the garden and locations. About 16 types of containers were used for growing plants. The total yield was recorded to be 135.38 kg per garden from 22 types of vegetables in the year of 2019. Among them, the highest yield was received from bottle gourd, 17.98 kg followed by tomato, 9.33 kg and country bean, 8.99 kg. In the case of fruits, the total yield was recorded as 77.24 kg per garden from 18 types of fruits in the same year. Among these, mango gave the highest yield (8.57 kg), followed by papaya (8.38 kg) and guava (7.52 kg). Research should be carried out on crop selection, fertilizer and irrigation management under different container systems and to develop a suitable RTG model for greening city of Bangladesh.

H7N9, a novel strain of avian origin influenza was the first recorded incidence where a human was transited by a N9 type influenza virus. Effective vaccination against influenza A (H7N9) is a major concern, since it has emerged as a life threatening viral pathogen. Here, an in silico reverse vaccinology strategy was adopted to design a unique chimeric subunit vaccine against avian influenza A (H7N9). Induction of humoral and cell-mediated immunity is the prime concerned characteristics for a peptide vaccine candidate, hence both T cell and B cell immunity of viral proteins were screened. Antigenicity testing, transmembrane topology screening, allergenicity and toxicity assessment, population coverage analysis and molecular docking approach were adopted to generate the most antigenic epitopes of avian influenza A (H7N9) proteome. Further, a novel subunit vaccine was designed by the combination of highly immunogenic epitopes along with suitable adjuvant and linkers. Physicochemical properties and secondary structure of the designed vaccine were assessed to ensure its thermostability, hydrophilicity, theoretical PI and structural behavior. Homology modeling, refinement and validation of the designed vaccine allowed to construct a three dimensional structure of the predicted vaccine, further employed to molecular docking analysis with different MHC molecules and human immune TLR8 receptor present on lymphocyte cells. Moreover, disulfide engineering was employed to lessen the high mobility region of the designed vaccine in order to extend its stability. Furthermore, we investigated the molecular dynamic simulation of the modeled subunit vaccine and TLR8 complexed molecule to strengthen our prediction. Finally, the suggested vaccine was reverse transcribed and adapted forE. colistrain K12 prior to insertion within pET28a(+) vector for checking translational potency and microbial expression.