12 results on '"Nicolas Morand"'
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2. Agut-Labordère D. et Redon B. (éd.), Les vaisseaux du désert et des steppes : les camélidés dans l’Antiquité
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Nicolas Morand
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Archaeology ,CC1-960 ,Ancient history ,D51-90 ,Medieval history ,D111-203 - Full Text
- View/download PDF
3. Active Nanofibrous Membrane Effects on Gingival Cell Inflammatory Response
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David-Nicolas Morand, Olivier Huck, Laetitia Keller, Nadia Jessel, Henri Tenenbaum, and Jean-Luc Davideau
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anti-inflammatory agents ,lipopolysaccharides ,fibroblasts ,epithelial cells ,polycaprolactone ,cytokines ,Technology ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Microscopy ,QH201-278.5 ,Descriptive and experimental mechanics ,QC120-168.85 - Abstract
Alpha-melanocyte stimulating hormone (α-MSH) is involved in normal skin wound healing and also has anti-inflammatory properties. The association of α-MSH to polyelectrolyte layers with various supports has been shown to improve these anti-inflammatory properties. This study aimed to evaluate the effects of nanofibrous membrane functionalized with α-MSH linked to polyelectrolyte layers on gingival cell inflammatory response. Human oral epithelial cells (EC) and fibroblasts (FB) were cultured on plastic or electrospun Poly-#-caprolactone (PCL) membranes with α-MSH covalently coupled to Poly-L-glutamic acid (PGA-α-MSH), for 6 to 24 h. Cells were incubated with or without Porphyromonas gingivalis lipopolysaccharide (Pg-LPS). Cell proliferation and migration were determined using AlamarBlue test and scratch assay. Expression of interleukin-6 (IL-6), tumor necrosis factor (TNF-α), and transforming growth factor-beta (TGF-β) was evaluated using RT-qPCR method. Cell cultures on plastic showed that PGA-α-MSH reduced EC and FB migration and decreased IL-6 and TGF-β expression in Pg-LPS stimulated EC. PGA-α-MSH functionalized PCL membranes reduced proliferation of Pg-LPS stimulated EC and FB. A significant decrease of IL-6, TNF-α, and TGF-β expression was also observed in Pg-LPS stimulated EC and FB. This study showed that the functionalization of nanofibrous PCL membranes efficiently amplified the anti-inflammatory effect of PGA-α-MSH on gingival cells.
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- 2015
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4. Sex in the city: Uncovering sex-specific management of equine resources from prehistoric times to the Modern Period in France
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Benoît Clavel, Sébastien Lepetz, Lorelei Chauvey, Stéphanie Schiavinato, Laure Tonasso-Calvière, Xuexue Liu, Antoine Fages, Naveed Khan, Andaine Seguin-Orlando, Clio Der Sarkissian, Pierre Clavel, Oscar Estrada, Duha Alioğlu, Charleen Gaunitz, Jean-Marc Aury, Maude Barme, Pierre Bodu, Monique Olive, Olivier Bignon-Lau, Jean-Christophe Castel, Myriam Boudadi-Maligne, Nicolas Boulbes, Alice Bourgois, Franck Decanter, Sylvain Foucras, Stéphane Frère, Armelle Gardeisen, Gaëtan Jouanin, Charlotte Méla, Nicolas Morand, Ariadna Nieto Espinet, Aude Perdereau, Olivier Putelat, Julie Rivière, Opale Robin, Marilyne Salin, Silvia Valenzuela-Lamas, Christian Vallet, Jean-Hervé Yvinec, Patrick Wincker, Ludovic Orlando, Ethnologie préhistorique, Archéologies et Sciences de l'Antiquité (ArScAn), Université Paris 1 Panthéon-Sorbonne (UP1)-Université Paris 8 Vincennes-Saint-Denis (UP8)-Université Paris Nanterre (UPN)-Ministère de la Culture et de la Communication (MCC)-Institut national de recherches archéologiques préventives (Inrap)-Centre National de la Recherche Scientifique (CNRS)-Université Paris 1 Panthéon-Sorbonne (UP1)-Université Paris 8 Vincennes-Saint-Denis (UP8)-Université Paris Nanterre (UPN)-Ministère de la Culture et de la Communication (MCC)-Institut national de recherches archéologiques préventives (Inrap)-Centre National de la Recherche Scientifique (CNRS), Technologie et Ethnologie des Mondes Préhistoriques (TEMPS), Université Paris 1 Panthéon-Sorbonne (UP1)-Université Paris Nanterre (UPN)-Centre National de la Recherche Scientifique (CNRS), Archéozoologie, archéobotanique : sociétés, pratiques et environnements (AASPE), Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS), Centre d'anthropologie et de génomique de Toulouse (CAGT), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Génomique métabolique (UMR 8030), Genoscope - Centre national de séquençage [Evry] (GENOSCOPE), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche Archéologique de la Vallée de l'Oise (CRAVO), Muséum national d'Histoire naturelle (MNHN), Université Paris 1 Panthéon-Sorbonne (UP1)-Université Paris 8 Vincennes-Saint-Denis (UP8)-Université Paris Nanterre (UPN)-Ministère de la Culture et de la Communication (MCC)-Centre National de la Recherche Scientifique (CNRS), Université Paris 1 Panthéon-Sorbonne (UP1)-Université Paris 8 Vincennes-Saint-Denis (UP8)-Université Paris Nanterre (UPN)-Ministère de la Culture et de la Communication (MCC)-Centre National de la Recherche Scientifique (CNRS)-Université Paris 1 Panthéon-Sorbonne (UP1)-Université Paris 8 Vincennes-Saint-Denis (UP8)-Université Paris Nanterre (UPN)-Ministère de la Culture et de la Communication (MCC)-Centre National de la Recherche Scientifique (CNRS), Museum d'Histoire Naturelle [Genève] (MHN), De la Préhistoire à l'Actuel : Culture, Environnement et Anthropologie (PACEA), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), Histoire naturelle de l'Homme préhistorique (HNHP), Muséum national d'Histoire naturelle (MNHN)-Université de Perpignan Via Domitia (UPVD)-Centre National de la Recherche Scientifique (CNRS), Institut national de recherches archéologiques préventives (Inrap), Archéologie et Archéométrie (ArAr), Université Lumière - Lyon 2 (UL2)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Archéologie des Sociétés Méditerranéennes (ASM), Université Paul-Valéry - Montpellier 3 (UPVM)-Centre National de la Recherche Scientifique (CNRS)-Ministère de la Culture (MC), Direction Archéologie et Muséum de la ville d'Aix-en-Provence, Centre Technique Municipal RTE des Milles Aix-en-Provence, Institut Royal des Sciences Naturelles de Belgique (IRSNB), Archéologie et histoire ancienne : Méditerranée - Europe (ARCHIMEDE), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Ministère de la Culture et de la Communication (MCC)-Centre National de la Recherche Scientifique (CNRS), Institución Milá y Fontanals de investigación en Humanidades (IMF), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), CEA- Saclay (CEA), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), ANR-10-INBS-0009,France-Génomique,Organisation et montée en puissance d'une Infrastructure Nationale de Génomique(2010), ANR-17-EURE-0010,CHESS,Toulouse Graduate School défis en économie et sciences sociales quantitatives(2017), European Project: 681605,PEGASUS, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Université Paris 1 Panthéon-Sorbonne (UP1)-Université Paris 8 Vincennes-Saint-Denis (UP8)-Université Paris Nanterre (UPN)-Ministère de la Culture et de la Communication (MCC)-Institut national de recherches archéologiques préventives (Inrap)-Centre National de la Recherche Scientifique (CNRS), Departamento de Arqueologı'a y Antropologı'a, Istitucio' Mila' i Fontanals, (IMF CSIC), Consejo Superior de Investigaciones Cientıficas (IMF-CSIC), Centre National de la Recherche Scientifique (France), European Research Council, France Génomique, Université de Toulouse, Villum Fonden, and Agence Nationale de la Recherche (France)
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Archeology ,Ancient DNA ,[SHS.ARCHEO]Humanities and Social Sciences/Archaeology and Prehistory ,Iron age ,City ,Breeding ,Horse ,Middle ages ,Roman period ,[SHS]Humanities and Social Sciences ,Donkey ,[SDE]Environmental Sciences ,Husbandry ,Hunting ,Mule ,Archaeozoology - Abstract
Sex identification from fragmentary archeozoological assemblages is particularly challenging in the Equid family, including for horses, donkeys and their hybrids. This limitation has precluded in-depth investigations of sex-ratio variation in various temporal, geographic and social contexts. Recently, shallow DNA sequencing has offered an economical solution to equine sex determination, even in environments where DNA preservation conditions is not optimal. In this study, we applied state-of-the-art methods in ancient DNA-based equine sex determination to 897 osseous remains in order to assess whether equal proportions of males and females could be found in a range of archeological contexts in France. We found Magdalenian horse hunt not focused on isolated bachelors, and Upper Paleolithic habitats and natural traps equally balancing sex ratios. In contrast, Iron Age sacrificial rituals appeared to have been preferentially oriented to male horses and this practice extended into the Roman Period. During Antiquity, the Middle Ages and the Modern Period, cities emerged as environments largely dominated by horse males. This strong sex-bias was considerably reduced, and sometimes even absent, in various rural contexts. Combined with previous archaeozoological work and textual evidence, our results portray an urban economy fueled by adult, often old, males, and rural environments where females and subadults of both sexes were maintained to sustain production demands., We thank Agnès Orsoni, Michela Leonardi, and Stefanie Wagner for lab assistance and all members of the AGES research team at CAGT for fruitful discussions. We also thank all archaeologists, curators and staff in charge of archaeological warehouses, who have facilitated access to the material analyzed in this study. Pierre Clavel’s PhD position is funded by the CNRS MITI interdisciplinary programme (‘Mission pour les Initiatives Transverses et Interdisciplinaires’). Xuexue Liu was supported by the European Union’s Horizon 2020 research and Innovation pro- gramme under the Marie Skłodowska-Curie grant agreement 101027750. This work was supported by the France G ́enomique Appel `a Grand Projet (ANR-10-INBS-09-08, BUCEPHALE project); the Initiative d’Excellence Chaires d’attractivit ́e, Universit ́e de Toulouse (OURASI) and the Villum Fonden miGENEPI research project. Andaine Seguin- Orlando acknowledges IAST for funding from ANR (France) under grant ANR-17-EURE-0010 (‘Investissements d’Avenir’ programme). This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement No. 681605).
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- 2022
5. Porphyromonas gingivalis Differentially Modulates Cell Death Profile in Ox-LDL and TNF-α Pre-Treated Endothelial Cells.
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Isaac Maximiliano Bugueno, Yacine Khelif, Narendra Seelam, David-Nicolas Morand, Henri Tenenbaum, Jean-Luc Davideau, and Olivier Huck
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Medicine ,Science - Abstract
OBJECTIVE:Clinical studies demonstrated a potential link between atherosclerosis and periodontitis. Porphyromonas gingivalis (Pg), one of the main periodontal pathogen, has been associated to atheromatous plaque worsening. However, synergism between infection and other endothelial stressors such as oxidized-LDL or TNF-α especially on endothelial cell (EC) death has not been investigated. This study aims to assess the role of Pg on EC death in an inflammatory context and to determine potential molecular pathways involved. METHODS:Human umbilical vein ECs (HUVECs) were infected with Pg (MOI 100) or stimulated by its lipopolysaccharide (Pg-LPS) (1μg/ml) for 24 to 48 hours. Cell viability was measured with AlamarBlue test, type of cell death induced was assessed using Annexin V/propidium iodide staining. mRNA expression regarding caspase-1, -3, -9, Bcl-2, Bax-1 and Apaf-1 has been evaluated with RT-qPCR. Caspases enzymatic activity and concentration of APAF-1 protein were evaluated to confirm mRNA results. RESULTS:Pg infection and Pg-LPS stimulation induced EC death. A cumulative effect has been observed in Ox-LDL pre-treated ECs infected or stimulated. This effect was not observed in TNF-α pre-treated cells. Pg infection promotes EC necrosis, however, in infected Ox-LDL pre-treated ECs, apoptosis was promoted. This effect was not observed in TNF-α pre-treated cells highlighting specificity of molecular pathways activated. Regarding mRNA expression, Pg increased expression of pro-apoptotic genes including caspases-1,-3,-9, Bax-1 and decreased expression of anti-apoptotic Bcl-2. In Ox-LDL pre-treated ECs, Pg increased significantly the expression of Apaf-1. These results were confirmed at the protein level. CONCLUSION:This study contributes to demonstrate that Pg and its Pg-LPS could exacerbate Ox-LDL and TNF-α induced endothelial injury through increase of EC death. Interestingly, molecular pathways are differentially modulated by the infection in function of the pre-stimulation.
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- 2016
- Full Text
- View/download PDF
6. Modification of poly(styrene‐b‐(ethylene‐co‐butylene)‐b‐styrene) via free‐radical grafting and its photo‐crosslinking
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Jean-Jacques Robin, Marcos Batistella, Thomas Brossier, Arnaud Regazzi, Sébastien Blanquer, Nicolas Morand, J.M. Lopez-Cuesta, Agnès Harlay, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Polymères Composites et Hybrides (PCH - IMT Mines Alès), IMT - MINES ALES (IMT - MINES ALES), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT), ARMOR, Durabilité des éco-Matériaux et Structures (DMS), Laboratoire de Mécanique et Génie Civil (LMGC), and Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-IMT - MINES ALES (IMT - MINES ALES)
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thermoplastic elastomers ,Ethylene ,Materials science ,Polymers and Plastics ,4-vinyl-1-cyclohexene 1 ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Styrene ,chemistry.chemical_compound ,[SPI]Engineering Sciences [physics] ,Polymer chemistry ,Photo crosslinking ,Materials Chemistry ,Thermoplastic elastomer ,technology, industry, and agriculture ,General Chemistry ,021001 nanoscience & nanotechnology ,Grafting ,0104 chemical sciences ,photo-crosslinking ,chemistry ,0210 nano-technology ,2-epoxide ,free-radical grafting ,poly(styrene-ethylene-butylene-styrene) SEBS - Abstract
International audience; Poly(styrene-b-(ethylene-co-butylene)-b-styrene) (SEBS) is functionalized via radical grafting with 4-vinyl-1-cyclohexene 1,2-epoxide (VCHO) initiated using 2,5-Bis(tert-butylperoxy)-2,5-dimethylhexane. The existence of the epoxide group on the SEBS backbone is evidenced by 1H nuclear magnetic resonance. A size exclusion chromatography (SEC) study reveals some chain coupling, and this phenomenon is limited by controlling the quantities of peroxide and monomer reagents used during the radical grafting. Photo-crosslinking of SEBS-g-VCHO under ultraviolet irradiation in the presence of a cationic initiator is then successfully performed with resultant gel contents higher than 85%. Mechanical properties of SEBS and crosslinked materials are measured by tensile tests on thin films. On the one hand, those tests reveal a significant increase of Young's modulus of the crosslinked materials. On the other hand, the diminution of elongation at break is much more limited; crosslinked materials retain their elastomeric properties with an elongation at break greater than 200%. Finally, the photosensitive SEBS-g-VCHO is used to show the adhesion performance of the photo-crosslinking coating as well as a resin for the stereolithography process.
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- 2021
7. Synthesis of a Novel Electrospun Polycaprolactone Scaffold Functionalized with Ibuprofen for Periodontal Regeneration: An In Vitro andIn Vivo Study
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Fareeha Batool, David-Nicolas Morand, Lionel Thomas, Isaac Maximiliano Bugueno, Javier Aragon, Silvia Irusta, Laetitia Keller, Nadia Benkirane-Jessel, Henri Tenenbaum, and Olivier Huck
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regeneration ,periodontitis ,membrane ,GTR ,NSAIDs ,Technology ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Microscopy ,QH201-278.5 ,Descriptive and experimental mechanics ,QC120-168.85 - Abstract
Ibuprofen (IBU) has been shown to improve periodontal treatment outcomes. The aim of this study was to develop a new anti-inflammatory scaffold by functionalizing an electrospun nanofibrous poly-ε-caprolactone membrane with IBU (IBU-PCL) and to evaluate its impact on periodontal inflammation, wound healing and regeneration in vitro and in vivo. IBU-PCL was synthesized through electrospinning. The effects of IBU-PCL on the proliferation and migration of epithelial cells (EC) and fibroblasts (FB) exposed to Porphyromonas gingivlais lipopolysaccharide (Pg-LPS) were evaluated through the AlamarBlue test and scratch assay, respectively. Anti-inflammatory and remodeling properties were investigated through Real time qPCR. Finally, the in vivo efficacy of the IBU-PCL membrane was assessed in an experimental periodontitis mouse model through histomorphometric analysis. The results showed that the anti-inflammatory effects of IBU on gingival cells were effectively amplified using the functionalized membrane. IBU-PCL reduced the proliferation and migration of cells challenged by Pg-LPS, as well as the expression of fibronectin-1, collagen-IV, integrin α3β1 and laminin-5. In vivo, the membranes significantly improved the clinical attachment and IBU-PCL also reduced inflammation-induced bone destruction. These data showed that the IBU-PCL membrane could efficiently and differentially control inflammatory and migratory gingival cell responses and potentially promote periodontal regeneration.
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- 2018
- Full Text
- View/download PDF
8. The exploitation of molluscs and other invertebrates in Alexandria (Egypt) from the Hellenistic period to Late antiquity
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Nicolas Morand, Centre de Recherche Archéologique de la Vallée de l'Oise (CRAVO), and Muséum national d'Histoire naturelle (MNHN)
- Subjects
[SHS.ARCHEO]Humanities and Social Sciences/Archaeology and Prehistory ,Alexandrie (Egypte) ,Hellenistic period ,Context (language use) ,Consumption (sociology) ,Late Antiquity ,Mediterranean sea ,époque hellénistique ,archéomalacologie ,ComputingMilieux_MISCELLANEOUS ,Taxonomy ,Invertebrate ,corail ,alimentation ,mollusques ,Excavation ,Biodiversity ,Archaeology ,commerce ,Basse Egypte ,Antiquité tardive ,Geography ,nacre ,Aquatic environment ,Anthropology ,Mer Méditerranée ,Animal Science and Zoology ,Mer Rouge - Abstract
A number of archaeological salvage excavations conducted in Alexandria (Lower Egypt) by the Centre d'Etudes alexandrines have provided a corpus of around 2000 fragments of marine, freshwater and terrestrial invertebrates. These archeomalacological remains come from several occupation layers of the same district within the town, the Brucheion, dating from the end of the 4th century BC until the 6th century AD. After macroscopic observations and through a binocular microscope, the analysis of the malacofauna vestiges has provided previously unknown information regarding the exploitation of the aquatic environment by Alexandrians during antiquity. In addition, some residues of mineral material preserved on the shells have been analysed under a scanning electron microscope (SEM). This study sheds light on both consumption choices and on the variety of uses for shells (container, decoration, raw material) within the Alexandrian domestic context. Certain species from the Red Sea and the Western Mediterranean Sea provide new data on the movement of products of marine origin within Ptolemaic and Roman Egypt.
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- 2020
9. Synthesis of a Novel Electrospun Polycaprolactone Scaffold Functionalized with Ibuprofen for Periodontal Regeneration: An In Vitro andIn Vivo Study
- Author
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Laetitia Keller, David-Nicolas Morand, Javier Aragón, Isaac Maximiliano Bugueno, Nadia Benkirane-Jessel, Henri Tenenbaum, Lionel Thomas, Silvia Irusta, Olivier Huck, Fareeha Batool, Regenerative NanoMedicine (UMR 1260), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Faculté de chirurgie dentaire - Strasbourg, Université de Strasbourg (UNISTRA), Département de Radiobiologie, Hadronthérapie et Imagerie Moléculaire (DRHIM-IPHC), Institut Pluridisciplinaire Hubert Curien (IPHC), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Department of chemical engineering INA University of Zaragoza, Hopitaux universitaires de Strasbourg Pole de médecine et chirurgie bucco-dentaire, Gaillard, Brigitte, and Université de Strasbourg (UNISTRA)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)
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Lipopolysaccharide ,NSAIDs ,[PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph] ,Cell ,Integrin ,02 engineering and technology ,lcsh:Technology ,Article ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,In vivo ,medicine ,General Materials Science ,lcsh:Microscopy ,periodontitis ,membrane ,ComputingMilieux_MISCELLANEOUS ,lcsh:QC120-168.85 ,Periodontitis ,[PHYS.PHYS.PHYS-BIO-PH] Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph] ,lcsh:QH201-278.5 ,biology ,lcsh:T ,Regeneration (biology) ,technology, industry, and agriculture ,030206 dentistry ,021001 nanoscience & nanotechnology ,medicine.disease ,In vitro ,Cell biology ,regeneration ,GTR ,medicine.anatomical_structure ,chemistry ,lcsh:TA1-2040 ,biology.protein ,lcsh:Descriptive and experimental mechanics ,lcsh:Electrical engineering. Electronics. Nuclear engineering ,lcsh:Engineering (General). Civil engineering (General) ,0210 nano-technology ,Wound healing ,lcsh:TK1-9971 - Abstract
Ibuprofen (IBU) has been shown to improve periodontal treatment outcomes. The aim of this study was to develop a new anti-inflammatory scaffold by functionalizing an electrospun nanofibrous poly-ε-caprolactone membrane with IBU (IBU-PCL) and to evaluate its impact on periodontal inflammation, wound healing and regeneration in vitro and in vivo. IBU-PCL was synthesized through electrospinning. The effects of IBU-PCL on the proliferation and migration of epithelial cells (EC) and fibroblasts (FB) exposed to Porphyromonas gingivlais lipopolysaccharide (Pg-LPS) were evaluated through the AlamarBlue test and scratch assay, respectively. Anti-inflammatory and remodeling properties were investigated through Real time qPCR. Finally, the in vivo efficacy of the IBU-PCL membrane was assessed in an experimental periodontitis mouse model through histomorphometric analysis. The results showed that the anti-inflammatory effects of IBU on gingival cells were effectively amplified using the functionalized membrane. IBU-PCL reduced the proliferation and migration of cells challenged by Pg-LPS, as well as the expression of fibronectin-1, collagen-IV, integrin α3β1 and laminin-5. In vivo, the membranes significantly improved the clinical attachment and IBU-PCL also reduced inflammation-induced bone destruction. These data showed that the IBU-PCL membrane could efficiently and differentially control inflammatory and migratory gingival cell responses and potentially promote periodontal regeneration.
- Published
- 2018
- Full Text
- View/download PDF
10. High frequency of Nichols-like strains and increased levels of macrolide resistance in Treponema pallidum in clinical samples from Buenos Aires, Argentina
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Nicolas Morando, Eliška Vrbová, Asunta Melgar, Roberto Daniel Rabinovich, David Šmajs, and María A. Pando
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Medicine ,Science - Abstract
Abstract Globally, 94% of Treponema pallidum subsp. pallidum (TPA) clinical strains belong to the SS14-like group and 6% to the Nichols-like group, with a prevalence of macrolide resistance of 90%. Our goal was to determine whether local TPA strain distribution and macrolide resistance frequency have changed significantly since our last report, which revealed that Buenos Aires had a high frequency of Nichols-like strains (27%) and low levels of macrolide resistance (14%). Swab samples from patients with suspected syphilis were collected during 2015–2019 and loci TP0136, TP0548, TP0705 were sequenced in order to perform multilocus sequence typing. Strains were classified as Nichols-like or SS14-like. The presence of macrolide resistance-associated mutations was determined by examination of the 23S rDNA gene sequence. Of 46 typeable samples, 37% were classified as Nichols-like and 63% as SS14-like. Macrolide resistance prevalence was 45.7%. Seven allelic profiles were found, five were SS14-like and two were Nichols-like. The frequency of Nichols-like strains increased between studies (26.8% vs. 37%, p = 0.36). A dramatic increase was found in the frequency of macrolide resistant strains between studies (14.3% vs. 45.7%, p = 0.005). Our results are in agreement with international trends and underscore the need to pursue further TPA molecular typing studies in South America.
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- 2022
- Full Text
- View/download PDF
11. Porphyromonas gingivalis Differentially Modulates Cell Death Profile in Ox-LDL and TNF-α Pre-Treated Endothelial Cells
- Author
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Olivier Huck, Jean-Luc Davideau, Henri Tenenbaum, Narendra Seelam, David-Nicolas Morand, Yacine Khelif, and Isaac Maximiliano Bugueno
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0301 basic medicine ,Lipopolysaccharides ,Aucun ,lcsh:Medicine ,Gene Expression ,Apoptosis ,030204 cardiovascular system & hematology ,Pathology and Laboratory Medicine ,Vascular Medicine ,chemistry.chemical_compound ,0302 clinical medicine ,Fluorescence Microscopy ,Medicine and Health Sciences ,Bacteroidaceae Infections ,lcsh:Science ,Immune Response ,Cells, Cultured ,bcl-2-Associated X Protein ,Staining ,Microscopy ,Multidisciplinary ,biology ,Cell Death ,Caspase 3 ,Caspase 1 ,Cell Staining ,Light Microscopy ,Caspase 9 ,Endothelial stem cell ,Lipoproteins, LDL ,Proto-Oncogene Proteins c-bcl-2 ,Cell Processes ,Tumor necrosis factor alpha ,Porphyromonas gingivalis ,Research Article ,Programmed cell death ,Imaging Techniques ,Cell Survival ,Immunology ,Research and Analysis Methods ,Necrotic Cell Death ,03 medical and health sciences ,Bcl-2-associated X protein ,Signs and Symptoms ,Diagnostic Medicine ,Fluorescence Imaging ,Genetics ,Human Umbilical Vein Endothelial Cells ,Humans ,Propidium iodide ,Viability assay ,Periodontitis ,Inflammation ,Tumor Necrosis Factor-alpha ,lcsh:R ,Biology and Life Sciences ,Cell Biology ,biology.organism_classification ,Atherosclerosis ,Molecular biology ,030104 developmental biology ,Apoptotic Protease-Activating Factor 1 ,chemistry ,Specimen Preparation and Treatment ,biology.protein ,lcsh:Q - Abstract
Objective Clinical studies demonstrated a potential link between atherosclerosis and periodontitis. Porphyromonas gingivalis (Pg), one of the main periodontal pathogen, has been associated to atheromatous plaque worsening. However, synergism between infection and other endothelial stressors such as oxidized-LDL or TNF-α especially on endothelial cell (EC) death has not been investigated. This study aims to assess the role of Pg on EC death in an inflammatory context and to determine potential molecular pathways involved. Methods Human umbilical vein ECs (HUVECs) were infected with Pg (MOI 100) or stimulated by its lipopolysaccharide (Pg-LPS) (1μg/ml) for 24 to 48 hours. Cell viability was measured with AlamarBlue test, type of cell death induced was assessed using Annexin V/propidium iodide staining. mRNA expression regarding caspase-1, -3, -9, Bcl-2, Bax-1 and Apaf-1 has been evaluated with RT-qPCR. Caspases enzymatic activity and concentration of APAF-1 protein were evaluated to confirm mRNA results. Results Pg infection and Pg-LPS stimulation induced EC death. A cumulative effect has been observed in Ox-LDL pre-treated ECs infected or stimulated. This effect was not observed in TNF-α pre-treated cells. Pg infection promotes EC necrosis, however, in infected Ox-LDL pre-treated ECs, apoptosis was promoted. This effect was not observed in TNF-α pre-treated cells highlighting specificity of molecular pathways activated. Regarding mRNA expression, Pg increased expression of pro-apoptotic genes including caspases-1,-3,-9, Bax-1 and decreased expression of anti-apoptotic Bcl-2. In Ox-LDL pre-treated ECs, Pg increased significantly the expression of Apaf-1. These results were confirmed at the protein level. Conclusion This study contributes to demonstrate that Pg and its Pg-LPS could exacerbate Ox-LDL and TNF-α induced endothelial injury through increase of EC death. Interestingly, molecular pathways are differentially modulated by the infection in function of the pre-stimulation.
- Published
- 2016
12. Active implant combining human stem cell microtissues and growth factors for bone-regenerative nanomedicine
- Author
-
Nadia Benkirane-Jessel, Laetitia Keller, Jean Christophe Lutz, Didier Mainard, Jessica Schiavi, Natalia de Isla, Pascale Schwinté, Olivier Huck, David-Nicolas Morand, Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Lausanne (UNIL), Université Paris-Est Marne-la-Vallée (UPEM), LAB'URBA (LAB'URBA), Université Paris-Est Marne-la-Vallée (UPEM)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Etude des Civilisations de l'Antiquité (UMR 7044), Centre National de la Recherche Scientifique (CNRS)-Université Marc Bloch - Strasbourg II-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA)), Institut Charles Sadron (ICS), Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Matériaux et nanosciences d'Alsace (FMNGE), Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Biomatériaux et ingénierie tissulaire, Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM), Immuno-Rhumatologie Moléculaire, Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Matériaux et nanosciences d'Alsace, Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Hôpital Central, Service de Chirurgie Orthopédique (UMR7561 CNRS), Inconnu, Université de Lausanne = University of Lausanne (UNIL), Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Université Marc Bloch - Strasbourg II-Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Matériaux et nanosciences d'Alsace (FMNGE), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, and Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Male ,MESH: Bone Morphogenetic Protein 7 ,Bone Morphogenetic Protein 7 ,Nanofibers ,Medicine (miscellaneous) ,Regenerative Medicine ,Regenerative medicine ,MESH: Tissue Engineering ,MESH: Cellular Microenvironment ,Human mesenchymal stem cells ,MESH: Bone Regeneration ,Mice ,BMP-7 ,Tissue engineering ,bone regeneration ,Osteogenesis ,MESH: Collagen ,MESH: Tissue Scaffolds ,General Materials Science ,MESH: Animals ,MESH: Osteogenesis ,Bone growth ,Tissue Scaffolds ,Cell Differentiation ,Anatomy ,[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM] ,Cell biology ,Bone morphogenetic protein 7 ,Nanomedicine ,medicine.anatomical_structure ,Cellular Microenvironment ,Collagen ,Stem cell ,MESH: Cell Differentiation ,Materials science ,Biomedical Engineering ,Mice, Nude ,Microtissues ,Bioengineering ,MESH: Bone Substitutes ,Development ,Mesenchymal Stem Cell Transplantation ,medicine ,MESH: Mice, Nude ,Animals ,Humans ,MESH: Mesenchymal Stem Cell Transplantation ,[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM] ,Bone regeneration ,MESH: Mice ,MESH: Humans ,Tissue Engineering ,Collagen nanofibers implant ,Mesenchymal stem cell ,[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,Regenerative nanomedicine ,MESH: Nanofibers ,MESH: Male ,MESH: Nanomedicine ,MESH: Regenerative Medicine ,Bone Substitutes ,Bone marrow ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology - Abstract
Aims: Mesenchymal stem cells (MSCs) from adult bone marrow provide an exciting and promising stem cell population for the repair of bone in skeletal diseases. Here, we describe a new generation of collagen nanofiber implant functionalized with growth factor BMP-7 nanoreservoirs and equipped with human MSC microtissues (MTs) for regenerative nanomedicine. Materials & methods: By using a 3D nanofibrous collagen membrane and by adding MTs rather than single cells, we optimize the microenvironment for cell colonization, differentiation and growth. Results & conclusion: Furthermore, in this study, we have shown that by combining BMP-7 with these MSC MTs in this double 3D environment, we further accelerate bone growth in vivo. The strategy described here should enhance the efficiency of therapeutic implants compared with current simplistic approaches used in the clinic today based on collagen implants soaked in bone morphogenic proteins.
- Published
- 2015
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