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Your search keyword '"Nicolai Sundgaard Bekker"' showing total 3 results
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3 results on '"Nicolai Sundgaard Bekker"'

1. Optimizing monosaccharide production from liquid hot water pretreatment and enzymatic hydrolysis of grass-clover press cake

Author

Nicolai Sundgaard Bekker, Saqib Sohail Toor, Kamaldeep Sharma, Thomas Helmer Pedersen, and Lars Haastrup Pedersen

Subjects
Liquid hot water, Enzymatic hydrolysis, Green biorefinery, Grass pulp, Agro-industrial residues, Monosaccharides, Science (General), Q1-390, Social sciences (General), H1-99
Abstract

In the present study, clover-grass press cake was treated by liquid hot water at temperatures of 180–200 °C for a reaction time of 5–10 min. Evaluation of pretreatments was based on the monosaccharide yield after enzymatic hydrolysis of the pretreated slurry and solid fraction, respectively. Extraction of up to 48% hemicellulose and 4% cellulose was observed during pretreatment. The optimal pretreatment conditions were identified as 190 °C and 10 min resulting in monosaccharide yields of 90% and 73% of the theoretical maximum by slurry and solid conversion, respectively. At optimal conditions, the C6 monosaccharide yield (83–90%) was fairly equal compared to the C5 monosaccharide yield (56–89%), which increased by slurry conversion due to near-complete monomerization of soluble xylo-oligosaccharides. In this study, we showed that clover-grass press cake possesses considerable potential as feedstock for production of fermentable sugars in a biorefinery context.

Published
2023
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2. Genetic potential for exopolysaccharide synthesis in activated sludge bacteria uncovered by genome-resolved metagenomics

Author

Morten Kam Dahl Dueholm, Maaike Besteman, Emil Juel Zeuner, Marie Riisgaard-Jensen, Morten Eneberg Nielsen, Sofie Zacho Vestergaard, Søren Heidelbach, Nicolai Sundgaard Bekker, and Per Halkjær Nielsen

Subjects
Exopolysaccharides, Environmental Engineering, WIMEK, Ecological Modeling, MicPhys, Pollution, Microbiology, Activated sludge, Microbiologie, Genome mining, EPS, Waste Management and Disposal, Water Science and Technology, Civil and Structural Engineering
Abstract

A good floc formation of activated sludge (AS) is crucial for solid-liquid separation and production of clean effluent during wastewater treatment. Floc formation is partly controlled by self-produced extracellular polymeric substances (EPS) such as exopolysaccharides, proteins, and nucleic acids. Little is known about the composition, structure, and function of EPS in AS and which bacteria produce them. To address this knowledge gap for the exopolysaccharides, we took advantage of 1083 high-quality metagenome-assembled genomes (MAGs) obtained from 23 Danish wastewater treatment plants. We investigated the genomic potential for exopolysaccharide biosynthesis in bacterial species typical in AS systems based on genome mining and gene synteny analyses. Putative gene clusters associated with the biosynthesis of alginate, cellulose, curdlan, diutan, hyaluronic acids, Pel, poly-β-1,6-N-acetyl-D-glucosamine (PNAG), Psl, S88 capsular polysaccharide, salecan, succinoglycan, and xanthan were identified and linked to individual MAGs, providing a comprehensive overview of the genome-resolved potential for these exopolysaccharides in AS bacteria. The approach and results provide a starting point for a more comprehensive understanding of EPS composition in wastewater treatment systems, which may facilitate a more refined regulation of the activated sludge process for improved stability.

Published
2023
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3. The effects of different potato dextrose agar media on secondary metabolite production in Fusarium

Author

Teis Esben Sondergaard, Emil Juel Zeuner, Morten Eneberg Nielsen, Katrine Hartmann Thomsen, Sofie Zacho Vestergaard, Jens Laurids Sørensen, Nicolai Sundgaard Bekker, Christian Kjær Olesen, Klaus Ringsborg Westphal, Sara Kramer Niebling, Søren Heidelbach, Marie Riisgaard-Jensen, and Jesper Skovmark

Subjects
Fusarium, Solanine, Metabolite, education, Secondary Metabolism, Biology, Secondary metabolite, Microbiology, 03 medical and health sciences, chemistry.chemical_compound, Nutrient, Potato dextrose agar, medicine, Food science, Mycotoxin, 030304 developmental biology, Solanum tuberosum, 0303 health sciences, 030306 microbiology, Secondary metabolites, food and beverages, General Medicine, Mycotoxins, biology.organism_classification, Culture Media, Agar, Glucose, chemistry, Composition (visual arts), Food Science, medicine.drug, Regulation
Abstract

Potatoes contain several nutrients essential for fungal growth, making them an excellent component of media such as the popular Potato Dextrose Agar (PDA) medium. Commercially, PDA is available from multiple retailers offering virtually the same product. These media, however, could contain small differences in composition of nutrients affecting the expression of secondary metabolites. This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum). Secondary metabolites were analysed using HPLC-HRMS, from which statistically significant differences in intensities were observed for 9 out of 10 metabolites.

Published
2021
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