Search

Pre-clinical and clinical studies revealed that mesenchymal stromal cell (MSC) transplants elicit tissue repair. Conditioning MSC prior to transplantation may boost their ability to support repair. We investigated macrophage-derived inflammation as a means to condition MSC by comprehensively analyzing their transcriptome and secretome. Conditioning MSC with macrophage-derived inflammation resulted in 3208 differentially expressed genes, which were annotated with significantly enriched GO terms for 1085 biological processes, 85 cellular components, and 79 molecular functions. Inflammation-mediated conditioning increased the secretion of growth factors that are key for tissue repair, including vascular endothelial growth factor, hepatocyte growth factor, nerve growth factor and glial-derived neurotrophic factor. Furthermore, we found that inflammation-mediated conditioning induces transcriptomic changes that challenge the viability and mobility of MSC. Our data support the notion that macrophage-derived inflammation stimulates MSC to augment their paracrine repair-supporting activity. The results suggest that inflammatory pre-conditioning enhances the therapeutic potential of MSC transplants.

Large national programs in the United States and several Asian countries have defined and characterised their marine methane hydrate occurrences in some detail, but European hydrate occurrence has received less attention. The European Union-funded project “Marine gas hydrate – an indigenous resource of natural gas for Europe” (MIGRATE) aimed to determine the European potential inventory of exploitable gas hydrate, to assess current technologies for their production, and to evaluate the associated risks. We present a synthesis of results from a MIGRATE working group that focused on the definition and assessment of hydrate in Europe. Our review includes the western and eastern margins of Greenland, the Barents Sea and onshore and offshore Svalbard, the Atlantic margin of Europe, extending south to the northwestern margin of Morocco, the Mediterranean Sea, the Sea of Marmara, and the western and southern margins of the Black Sea. We have not attempted to cover the high Arctic, the Russian, Ukrainian and Georgian sectors of the Black Sea, or overseas territories of European nations. Following a formalised process, we defined a range of indicators of hydrate presence based on geophysical, geochemical and geological data. Our study was framed by the constraint of the hydrate stability field in European seas. Direct hydrate indicators included sampling of hydrate; the presence of bottom simulating reflectors in seismic reflection profiles; gas seepage into the ocean; and chlorinity anomalies in sediment cores. Indirect indicators included geophysical survey evidence for seismic velocity and/or resistivity anomalies, seismic reflectivity anomalies or subsurface gas escape structures; various seabed features associated with gas escape, and the presence of an underlying conventional petroleum system. We used these indicators to develop a database of hydrate occurrence across Europe. We identified a series of regions where there is substantial evidence for hydrate occurrence (some areas offshore Greenland, offshore west Svalbard, the Barents Sea, the midNorwegian margin, the Gulf of Cadiz, parts of the eastern Mediterranean, the Sea of Marmara and the Black Sea) and regions where the evidence is more tenuous (other areas offshore Greenland and of the eastern Mediterranean, onshore Svalbard, offshore Ireland and offshore northwest Iberia). We provide an overview of the evidence for hydrate occurrence in each of these regions. We conclude that around Europe, areas with strong evidence for the presence of hydrate commonly coincide with conventional thermogenic hydrocarbon provinces., Sí

The relatively high installation costs for different types of shallow geothermal energy systems are obstacles that have lowered the impact of geothermal solutions in the renewable energy market. In order to reduce planning costs and obtain a lithological overview of geothermal potentials and drilling conditions, a pan-European geological overview map was created using freely accessible JRC (Joint Research Centre) data and ArcGIS software. JRC data were interpreted and merged together in order to collect information about the expenditure of installing geothermal systems in specific geological set-ups, and thereby select the most economic drilling technique. Within the four-year project of the European Union’s Horizon 2020 Research and Innovation Program, which is known as “Cheap-GSHPs” (the Cheap and efficient application of reliable Ground Source Heat exchangers and Pumps), the most diffused lithologies and corresponding drilling costs were analyzed to provide a 1 km × 1 km raster with the required underground information. The final outline map should be valid throughout Europe, and should respect the INSPIRE (INfrastructure for SPatial InfoRmation in Europe) guidelines.

Sustained ocean observations are crucial to understand both natural processes occurring in the ocean and human influence on the marine ecosystems. The information they provide increases our understanding and is therefore beneficial to the society as a whole because it contributes to a more efficient use and protection of the marine environment, upon which human livelihood depends. In addition the oceans, which occupy 73% of the planet surface and host 93% of the biosphere, play a massive role in controlling the climate. Eulerian or fixed-point observatories are an essential component of the global ocean observing system as they provide several unique features that cannot be found in other systems and are therefore complementary to them. In addition they provide a unique opportunity for multidisciplinary and interdisciplinary work, combining physical, chemical and biological observations on several time scales. The fixed-point open ocean observatory network (FixO3) integrates the 23 European open ocean fixed-point observatories in the Atlantic Ocean and in the Mediterranean Sea. The programme also seeks to improve access to key installations and the knowledge they provide for the wider community, from scientists, to businesses, to civil society. This paper summarises the rationale behind open ocean observatories monitoring the essential ocean variables. It also provides an estimate of the costs to operate a typical fixed-point observatory such as those included in the FixO3 network. Finally an assessment of the type of data and services provided by ocean observations and their value to society is also given.