27 results on '"Ngandjio A"'
Search Results
2. Genomic history of the seventh pandemic of cholera in Africa
- Author
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Weill, François-Xavier, Domman, Daryl, Njamkepo, Elisabeth, Tarr, Cheryl, Rauzier, Jean, Fawal, Nizar, Keddy, Karen H., Salje, Henrik, Moore, Sandra, Mukhopadhyay, Asish K., Bercion, Raymond, Luquero, Francisco J., Ngandjio, Antoinette, Dosso, Mireille, Monakhova, Elena, Garin, Benoit, Bouchier, Christiane, Pazzani, Carlo, Mutreja, Ankur, Grunow, Roland, Sidikou, Fati, Bonte, Laurence, Breurec, Sébastien, Damian, Maria, Njanpop-Lafourcade, Berthe-Marie, Sapriel, Guillaume, Page, Anne-Laure, Hamze, Monzer, Henkens, Myriam, Chowdhury, Goutam, Mengel, Martin, Koeck, Jean-Louis, Fournier, Jean-Michel, Dougan, Gordon, Grimont, Patrick A. D., Parkhill, Julian, Holt, Kathryn E., Piarroux, Renaud, Ramamurthy, Thandavarayan, Quilici, Marie-Laure, and Thomson, Nicholas R.
- Published
- 2017
3. First report of New Delhi metallo-beta-lactamase-5 (NDM-5)-producing Escherichia coli isolates from water environment in Cameroon
- Author
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Megueya, Armelle Leslie, Makuetche, Kévine, Scaccia, Nazareno, Costa, Silvia Figueiredo, da Silva Foncesca, Joyce Vanessa, Komurian-Pradel, Florence, Tchatchouang, Serges, Simo, Pierrette, Tchatchueng, Jules, Nono, Arsène Djoko, Ngang, Essia, Ngandjio, Antoinette, and Nzouankeu, Ariane
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- 2024
- Full Text
- View/download PDF
4. Author Correction: Genomic analysis of sewage from 101 countries reveals global landscape of antimicrobial resistance (Nature Communications, (2022), 13, 1, (7251), 10.1038/s41467-022-34312-7)
- Author
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Munk, Patrick, Brinch, Christian, Møller, Frederik Duus, Petersen, Thomas N., Hendriksen, Rene S., Seyfarth, Anne Mette, Kjeldgaard, Jette S., Svendsen, Christina Aaby, van Bunnik, Bram, Berglund, Fanny, Bego, Artan, Power, Pablo, Rees, Catherine, Lambrinidis, Dionisia, Neilson, Elizabeth Heather Jakobsen, Gibb, Karen, Coventry, Kris, Collignon, Peter, Cassar, Susan, Allerberger, Franz, Begum, Anowara, Hossain, Zenat Zebin, Worrell, Carlon, Vandenberg, Olivier, Pieters, Ilse, Victorien, Dougnon Tamègnon, Gutierrez, Angela Daniela Salazar, Soria, Freddy, Grujić, Vesna Rudić, Mazalica, Nataša, Rahube, Teddie O., Tagliati, Carlos Alberto, Rodrigues, Dalia, Oliveira, Guilherme, de Souza, Larissa Camila Ribeiro, Ivanov, Ivan, Juste, Bonkoungou Isidore, Oumar, Traoré, Sopheak, Thet, Vuthy, Yith, Ngandjio, Antoinette, Nzouankeu, Ariane, Olivier, Ziem A.Abah Jacques, Yost, Christopher K., Kumar, Pratik, Brar, Satinder Kaur, Tabo, Djim Adjim, Adell, Aiko D., Paredes-Osses, Esteban, Koopmans, Marion, and Virology
- Abstract
In this article, the author name Antoinette Ngandjio was incorrectly written as Antoinette Ngandijo. In this article, the affiliation details for Author Sara Cuadros-Orellana were incorrectly given as ‘Centro de Biotecnologνa de los Recursos Naturales, Facultad de Ciencias Agrarias y Forestales, Talca, Chile’ but should have been ‘Universidad Catolica del Maule, Centro de Biotecnología de los Recursos Naturales, Facultad de Ciencias Agrarias y Forestales, Talca, Chile’. The original article has been corrected.
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- 2023
5. Multidrug-Resistant Salmonella enterica Serotype Typhi, Gulf of Guinea Region, Africa
- Author
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Murielle Baltazar, Antoinette Ngandjio, Kathryn Elizabeth Holt, Elodie Lepillet, Maria Pardos de la Gandara, Jean-Marc Collard, Raymond Bercion, Ariane Nzouankeu, Simon Le Hello, Gordon Dougan, Marie-Christine Fonkoua, and François-Xavier Weill
- Subjects
Salmonella enterica serotype Typhi ,bacteria ,multidrug resistance ,MDR ,pulsed-field gel electrophoresis ,PFGE ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Abstract
We identified 3 lineages among multidrug-resistant (MDR) Salmonella enterica serotype Typhi isolates in the Gulf of Guinea region in Africa during the 2000s. However, the MDR H58 haplotype, which predominates in southern Asia and Kenya, was not identified. MDR quinolone-susceptible isolates contained a 190-kb incHI1 pST2 plasmid or a 50-kb incN pST3 plasmid.
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- 2015
- Full Text
- View/download PDF
6. Histoplasmosis in HIV-Infected Persons, Yaoundé, Cameroon
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Christine E. Mandengue, Antoinette Ngandjio, and Paul J.A. Atangana
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Cameroon ,HIV/AIDS and other retroviruses ,tuberculosis ,histoplasmosis ,fungi ,viruses ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Published
- 2015
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- View/download PDF
7. Klebsiella pneumoniae resistant to third-generation cephalosporins in five African and two Vietnamese major towns: multiclonal population structure with two major international clonal groups, CG15 and CG258
- Author
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Breurec, S., Guessennd, N., Timinouni, M., Le, T.T.H., Cao, V., Ngandjio, A., Randrianirina, F., Thiberge, J.M., Kinana, A., Dufougeray, A., Perrier-Gros-Claude, J.D., Boisier, P., Garin, B., and Brisse, S.
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- 2013
- Full Text
- View/download PDF
8. The Lake Chad Basin, an Isolated and Persistent Reservoir of Vibrio cholerae O1: A Genomic Insight into the Outbreak in Cameroon, 2010.
- Author
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Rolf S Kaas, Antoinette Ngandjio, Ariane Nzouankeu, Achiraya Siriphap, Marie-Christine Fonkoua, Frank M Aarestrup, and Rene S Hendriksen
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Medicine ,Science - Abstract
The prevalence of reported cholera was relatively low around the Lake Chad basin until 1991. Since then, cholera outbreaks have been reported every couple of years. The objective of this study was to investigate the 2010/2011 Vibrio cholerae outbreak in Cameroon to gain insight into the genomic make-up of the V. cholerae strains responsible for the outbreak. Twenty-four strains were isolated and whole genome sequenced. Known virulence genes, resistance genes and integrating conjugative element (ICE) elements were identified and annotated. A global phylogeny (378 genomes) was inferred using a single nucleotide polymorphism (SNP) analysis. The Cameroon outbreak was found to be clonal and clustered distant from the other African strains. In addition, a subset of the strains contained a deletion that was found in the ICE element causing less resistance. These results suggest that V. cholerae is endemic in the Lake Chad basin and different from other African strains.
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- 2016
- Full Text
- View/download PDF
9. Trends of Antibacterial Resistance at the National Reference Laboratory in Cameroon: Comparison of the Situation between 2010 and 2017
- Author
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Marie-Christine Fonkoua, M. Massongo, W. Mbanzouen, D. Barger, L. Ngando, Ariane Nzouankeu, J. Tchatchueng, E.W. Pefura Yone, M. C. Tejiokem, A. Ngandjio, Université de Yaoundé I, Jamot hospital, Service de bactériologie [Yaoundé, Cameroun], Centre Pasteur du Cameroun, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Laboratoire d'Epidémiologie et de Santé Publique, Bordeaux population health (BPH), and Université de Bordeaux (UB)-Institut de Santé Publique, d'Épidémiologie et de Développement (ISPED)-Institut National de la Santé et de la Recherche Médicale (INSERM)
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0301 basic medicine ,Adult ,Male ,Veterinary medicine ,Salmonella ,Article Subject ,Adolescent ,medicine.drug_class ,030106 microbiology ,Antibiotics ,Drug resistance ,Microbial Sensitivity Tests ,medicine.disease_cause ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,0302 clinical medicine ,Antibiotic resistance ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,Drug Resistance, Bacterial ,medicine ,Humans ,030212 general & internal medicine ,Cameroon ,Medical prescription ,Child ,Retrospective Studies ,General Immunology and Microbiology ,biology ,Bacteria ,Pseudomonas aeruginosa ,business.industry ,Infant ,General Medicine ,Bacterial Infections ,Acinetobacter ,Middle Aged ,biology.organism_classification ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,3. Good health ,Anti-Bacterial Agents ,Multiple drug resistance ,Child, Preschool ,Medicine ,Female ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,business ,Research Article - Abstract
Introduction. Antimicrobial resistance represents a growing public health threat. One of the World Health Organization’s strategic objectives is “strengthening knowledge through surveillance and research.” Sub-Saharan African countries are still far from achieving this objective. We aimed to estimate and compare the prevalence of antibacterial resistance in 2010 and 2017 in Cameroon. Methods. We conducted a retrospective study on all clinical specimens cultured in Centre Pasteur du Cameroun (CPC) in 2010 and 2017. Data were extracted from the CPC’s laboratory data information system software and then managed and analyzed using R. Bacterial resistance rates were calculated in each year and compared using chi-square or Fisher’s tests, and relative changes were calculated. Outcomes included acquired resistance (AR), WHO priority resistant pathogens, some specific resistances of clinical interest, and resistance patterns (multi, extensively, and pan drug resistances) for five selected pathogens. Results. A total of 10,218 isolates were analyzed. The overall AR rate was 96.0% (95% CI: 95.4–96.6). Most of WHO priority bacterial resistance rates increased from 2010 to 2017. The most marked increases expressed as relative changes concerned imipenem-resistant Acinetobacter (6.2% vs. 21.6%, +248.4%, p = 0.02 ), imipenem-resistant Pseudomonas aeruginosa (13.5% vs. 23.5%, +74.1%, p < 0.01 ), 3rd generation-resistant Enterobacteriaceae (23.8% vs. 40.4%, +65.8%, p < 10 − 15 ), methicillin-resistant Staphylococcus aureus (27.3% vs. 46.0%, +68.6%, p < 0.002 ), fluoroquinolone-resistant Salmonella (3.9% vs. 9.5%, +142.9%, p = 0.03 ), and fluoroquinolone-resistant Enterobacteriaceae (32.6% vs. 54.0%, +65.8%, p < 10 − 15 ). For selected pathogens, global multidrug resistance was high in 2010 and 2017 (74.9% vs. 78.0% +4.1%, p = 0.01 ), intensively drug resistance rate was 5.8% (7.0% vs. 4.7%; p = 0.07 ), and no pan drug resistance has been identified. Conclusion. Bacterial resistance to antibiotics of clinical relevance in Cameroon was high and appeared to increase between 2010 and 2017. There is a need for regular surveillance of antibacterial resistance to inform public health strategies and empirically inform prescription practices.
- Published
- 2021
10. Global phylogeography and evolutionary history of Shigella dysenteriae type 1
- Author
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Njamkepo, Elisabeth, Fawal, Nizar, Tran-Dien, Alicia, Hawkey, Jane, Strockbine, Nancy, Jenkins, Claire, Talukder, Kaisar A., Bercion, Raymond, Kuleshov, Konstantin, Kolínská, Renáta, Russell, Julie E., Kaftyreva, Lidia, Accou-Demartin, Marie, Karas, Andreas, Vandenberg, Olivier, Mather, Alison E., Mason, Carl J., Page, Andrew J., Ramamurthy, Thandavarayan, Bizet, Chantal, Gamian, Andrzej, Carle, Isabelle, Sow, Amy Gassama, Bouchier, Christiane, Wester, Astrid Louise, Lejay-Collin, Monique, Fonkoua, Marie-Christine, Le Hello, Simon, Blaser, Martin J., Jernberg, Cecilia, Ruckly, Corinne, Mérens, Audrey, Page, Anne-Laure, Aslett, Martin, Roggentin, Peter, Fruth, Angelika, Denamur, Erick, Venkatesan, Malabi, Bercovier, Hervé, Bodhidatta, Ladaporn, Chiou, Chien-Shun, Clermont, Dominique, Colonna, Bianca, Egorova, Svetlana, Pazhani, Gururaja P., Ezernitchi, Analia V., Guigon, Ghislaine, Harris, Simon R., Izumiya, Hidemasa, Korzeniowska-Kowal, Agnieszka, Lutyńska, Anna, Gouali, Malika, Grimont, Francine, Langendorf, Céline, Marejková, Monika, Peterson, Lorea A.M., Perez-Perez, Guillermo, Ngandjio, Antoinette, Podkolzin, Alexander, Souche, Erika, Makarova, Mariia, Shipulin, German A., Ye, Changyun, Žemličková, Helena, Herpay, Mária, Grimont, Patrick A. D., Parkhill, Julian, Sansonetti, Philippe, Holt, Kathryn E., Brisse, Sylvain, Thomson, Nicholas R., and Weill, François-Xavier
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- 2016
- Full Text
- View/download PDF
11. Histoplasmosis in HIV-infected persons, Yaounde, Cameroon
- Author
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Mandengue, Christine E., Ngandjio, Antoinette, and Atangana, Paul J.A.
- Subjects
HIV patients -- Health aspects ,Histoplasmosis -- Health aspects ,Health - Abstract
To the Editor: In HIV-infected persons in Cameroon (Central Africa), histoplasmosis is still misdiagnosed as tuberculosis because of clinical similarities (1,2). These patients are automatically given presumptive antituberculous therapy, although [...]
- Published
- 2015
12. Emergence of multi-drug resistant Salmonella enterica serotype Stanleyville infections among children in Yaounde, Cameroon
- Author
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Ngandjio, Antoinette, Tchendjou, Patrice, Koki Ndombo, Paul, Gonsu Kamga, Hortense, and Fonkoua, Marie-Christine
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- 2012
- Full Text
- View/download PDF
13. Restriction endonuclease patterns of the omp1 gene of reference Chlamydia trachomatis strains and characterization of isolates from Cameroonian students
- Author
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Ngandjio, Antoinette, Clerc, Maithe, Fonkoua, Marie Christine, Thonnon, Jocelyn, Lunel, Françoise, Bébéar, Christiane, Bianchi, Anne, and de Barbeyrac, Bertille
- Published
- 2004
14. Trends of Antibacterial Resistance at the National Reference Laboratory in Cameroon: Comparison of the Situation between 2010 and 2017.
- Author
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Massongo, M., Ngando, L., Pefura Yone, E. W., NZouankeu, Ariane, Mbanzouen, W., Fonkoua, M. C., Ngandjio, A., Tchatchueng, J., Barger, D., and Tejiokem, M. C.
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CONFIDENCE intervals ,LABORATORIES ,RETROSPECTIVE studies ,FISHER exact test ,DISEASE prevalence ,CHI-squared test ,DESCRIPTIVE statistics ,DRUG resistance in microorganisms ,DATA analysis software - Abstract
Introduction. Antimicrobial resistance represents a growing public health threat. One of the World Health Organization's strategic objectives is "strengthening knowledge through surveillance and research." Sub-Saharan African countries are still far from achieving this objective. We aimed to estimate and compare the prevalence of antibacterial resistance in 2010 and 2017 in Cameroon. Methods. We conducted a retrospective study on all clinical specimens cultured in Centre Pasteur du Cameroun (CPC) in 2010 and 2017. Data were extracted from the CPC's laboratory data information system software and then managed and analyzed using R. Bacterial resistance rates were calculated in each year and compared using chi-square or Fisher's tests, and relative changes were calculated. Outcomes included acquired resistance (AR), WHO priority resistant pathogens, some specific resistances of clinical interest, and resistance patterns (multi, extensively, and pan drug resistances) for five selected pathogens. Results. A total of 10,218 isolates were analyzed. The overall AR rate was 96.0% (95% CI: 95.4–96.6). Most of WHO priority bacterial resistance rates increased from 2010 to 2017. The most marked increases expressed as relative changes concerned imipenem-resistant Acinetobacter (6.2% vs. 21.6%, +248.4%, p = 0.02), imipenem-resistant Pseudomonas aeruginosa (13.5% vs. 23.5%, +74.1%, p < 0.01), 3rd generation-resistant Enterobacteriaceae (23.8% vs. 40.4%, +65.8%, p < 10 − 15 ), methicillin-resistant Staphylococcus aureus (27.3% vs. 46.0%, +68.6%, p < 0.002), fluoroquinolone-resistant Salmonella (3.9% vs. 9.5%, +142.9%, p = 0.03), and fluoroquinolone-resistant Enterobacteriaceae (32.6% vs. 54.0%, +65.8%, p < 10 − 15 ). For selected pathogens, global multidrug resistance was high in 2010 and 2017 (74.9% vs. 78.0% +4.1%, p = 0.01), intensively drug resistance rate was 5.8% (7.0% vs. 4.7%; p = 0.07), and no pan drug resistance has been identified. Conclusion. Bacterial resistance to antibiotics of clinical relevance in Cameroon was high and appeared to increase between 2010 and 2017. There is a need for regular surveillance of antibacterial resistance to inform public health strategies and empirically inform prescription practices. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
15. Characterisation of class 3 integrons with oxacillinase gene cassettes in hospital sewage and sludge samples from France and Luxembourg
- Author
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Simo Tchuinte, Pierrette Landrie, Stalder, Thibault, Venditti, Silvia, Ngandjio, Antoinette, Dagot, Christophe, Ploy, Marie-Cécile, and Barraud, Olivier
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- 2016
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16. The Lake Chad Basin, an Isolated and Persistent Reservoir of Vibrio cholerae O1: A Genomic Insight into the Outbreak in Cameroon, 2010
- Author
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Frank Møller Aarestrup, Antoinette Ngandjio, Rene S. Hendriksen, Achiraya Siriphap, Ariane Nzouankeu, Marie-Christine Fonkoua, and Rolf Sommer Kaas
- Subjects
Bacterial Diseases ,0301 basic medicine ,Disease reservoir ,Marine and Aquatic Sciences ,lcsh:Medicine ,Plant Science ,Pathology and Laboratory Medicine ,Plant Genetics ,medicine.disease_cause ,El Tor ,Genome ,Disease Outbreaks ,Geographical Locations ,Cholera ,Medicine and Health Sciences ,Plant Genomics ,Cameroon ,lcsh:Science ,Phylogeny ,Genetics ,Multidisciplinary ,biology ,Vibrio cholerae O1 ,Genomics ,Anti-Bacterial Agents ,Bacterial Pathogens ,Infectious Diseases ,Medical Microbiology ,Vibrio cholerae ,Pathogens ,Research Article ,Neglected Tropical Diseases ,Freshwater Environments ,Biotechnology ,Genotype ,Chad ,Microbial Sensitivity Tests ,Serogroup ,History, 21st Century ,Polymorphism, Single Nucleotide ,Microbiology ,03 medical and health sciences ,SDG 3 - Good Health and Well-being ,Phylogenetics ,Microbial Control ,Vibrio Cholerae ,parasitic diseases ,medicine ,Humans ,Microbial Pathogens ,Disease Reservoirs ,Vibrio ,Pharmacology ,Bacteria ,Ecology and Environmental Sciences ,lcsh:R ,Organisms ,Biology and Life Sciences ,Aquatic Environments ,Outbreak ,Sequence Analysis, DNA ,Bodies of Water ,Tropical Diseases ,medicine.disease ,biology.organism_classification ,Lakes ,030104 developmental biology ,People and Places ,Africa ,Earth Sciences ,Multilocus sequence typing ,Plant Biotechnology ,lcsh:Q ,Antimicrobial Resistance ,Genome, Bacterial ,Multilocus Sequence Typing - Abstract
The prevalence of reported cholera was relatively low around the Lake Chad basin until 1991. Since then, cholera outbreaks have been reported every couple of years. The objective of this study was to investigate the 2010/2011 Vibrio cholerae outbreak in Cameroon to gain insight into the genomic make-up of the V. cholerae strains responsible for the outbreak. Twenty-four strains were isolated and whole genome sequenced. Known virulence genes, resistance genes and integrating conjugative element (ICE) elements were identified and annotated. A global phylogeny (378 genomes) was inferred using a single nucleotide polymorphism (SNP) analysis. The Cameroon outbreak was found to be clonal and clustered distant from the other African strains. In addition, a subset of the strains contained a deletion that was found in the ICE element causing less resistance. These results suggest that V. cholerae is endemic in the Lake Chad basin and different from other African strains.
- Published
- 2016
17. Multidrug-Resistant Salmonella enterica Serotype Typhi, Gulf of Guinea Region, Africa
- Author
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Pardos de la Gandara, Maria, Baltazar, Murielle, Ngandjio, Antoinette, Holt, Kathryn Elizabeth, Lepillet, Elodie, Pardos De La Gándara, María, Collard, Jean-Marc, Bercion, Raymond, Nzouankeu, Ariane, Le Hello, Simon, Dougan, Gordon, Fonkoua, Marie-Christine, Weill, François-Xavier, Institut Pasteur [Paris], Centre Pasteur du Cameroun, Réseau International des Instituts Pasteur (RIIP), University of Melbourne, The Wellcome Trust Sanger Institute [Cambridge], Centre National de Référence - National Reference Center Escherichia coli, Shigella et Salmonella (CNR-ESS), Institut Scientifique de Santé Publique [Belgique] - Scientific Institute of Public Health [Belgium] (WIV-ISP), Institut Pasteur de Bangui, This study was supported by the Institut Pasteur, the Réseau International des Instituts Pasteur, the Institut de Veille Sanitaire, and the French Government Investissement d’Avenir Program (Integrative Biology of Emerging Infectious Diseases, Laboratory of Excellence, grant ANR-10-LABX-62-IBEID)., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), and Institut Pasteur [Paris] (IP)
- Subjects
Serotype ,MESH: Geography ,Epidemiology ,lcsh:Medicine ,Salmonella enterica serotype Typhi ,Drug resistance ,Salmonella typhi ,Plasmid ,Drug Resistance, Multiple, Bacterial ,MDR ,MESH: Genetic Variation ,bacteria ,0303 health sciences ,MESH: Guinea ,MESH: Microbial Sensitivity Tests ,Geography ,biology ,MESH: Polymorphism, Single Nucleotide ,MESH: Salmonella typhi ,Dispatch ,PFGE ,Anti-Bacterial Agents ,Infectious Diseases ,Salmonella enterica ,pulsed-field gel electrophoresis ,Microbiology (medical) ,Microbial Sensitivity Tests ,Polymorphism, Single Nucleotide ,Typhoid fever ,Microbiology ,lcsh:Infectious and parasitic diseases ,03 medical and health sciences ,multidrug resistance ,MESH: Anti-Bacterial Agents ,medicine ,Humans ,lcsh:RC109-216 ,MESH: Typhoid Fever ,Typhoid Fever ,030304 developmental biology ,Multidrug-Resistant Salmonella enterica Serotype Typhi, Gulf of Guinea Region, Africa ,MESH: Humans ,030306 microbiology ,Haplotype ,lcsh:R ,Genetic Variation ,MESH: Drug Resistance, Multiple, Bacterial ,MESH: Haplotypes ,biology.organism_classification ,medicine.disease ,Virology ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,Multiple drug resistance ,Haplotypes ,Guinea ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie - Abstract
International audience; We identified 3 lineages among multidrug-resistant (MDR) Salmonella enterica serotype Typhi isolates in the Gulf of Guinea region in Africa during the 2000s. However, the MDR H58 haplotype, which predominates in southern Asia and Kenya, was not identified. MDR quinolone-susceptible isolates contained a 190-kb incHI1 pST2 plasmid or a 50-kb incN pST3 plasmid.
- Published
- 2015
18. Klebsiella pneumoniae resistant to third-generation cephalosporins in five African and two Vietnamese major towns: multiclonal population structure with two major international clonal groups, CG15 and CG258
- Author
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Jean-David Perrier-Gros-Claude, V Cao, T A H Le, Benoit Garin, N Guessennd, Sylvain Brisse, M Timinouni, A Ngandjio, Jean-Michel Thiberge, Frédérique Randrianirina, Alfred Dieudonné Kinana, P. Boisier, A. Dufougeray, Sébastien Breurec, Institut Pasteur de Dakar, Réseau International des Instituts Pasteur (RIIP), Institut Pasteur de Côte d'Ivoire, Institut Pasteur du Maroc, Institut National d'Hygiène et d'Épidémiologie de Hanoi (NIHE), Institut Pasteur d'Ho Chi Minh Ville, Centre Pasteur du Cameroun, Institut Pasteur de Madagascar, Génotypage des Pathogènes et Santé Publique (Plate-forme) (PF8), Institut Pasteur [Paris], We thank Fatou Bintou Dieye (Institut Pasteur, Dakar, Senegal), Laure Diancourt and Patrice Courvalin (Institut Pasteur, Paris, France), Guillaume Arlet (Hoˆ pital Tenon, Paris, France) and Marie Christine Ploy (CHU Dupuytren, Limoges, France) for their contributions, as well as all the clinicians involved in this study., and Institut Pasteur [Paris] (IP)
- Subjects
Microbiology (medical) ,CTX-M-15 ,Veterinary medicine ,Genotype ,Klebsiella pneumoniae ,multilocus sequence typing ,beta-Lactam Resistance ,Microbiology ,03 medical and health sciences ,Intergenic region ,Antibiotic resistance ,Genetic variation ,parasitic diseases ,Humans ,antimicrobial resistance ,Developing Countries ,030304 developmental biology ,CG15 ,0303 health sciences ,Genetic diversity ,Molecular Epidemiology ,Molecular epidemiology ,biology ,030306 microbiology ,1. No poverty ,Genetic Variation ,General Medicine ,extended-spectrum β-lactamase ,biology.organism_classification ,extended-spectrum b-lactamase ,3. Good health ,Anti-Bacterial Agents ,Cephalosporins ,Klebsiella Infections ,Infectious Diseases ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,Vietnam ,Genes, Bacterial ,Africa ,CG258 ,Multilocus sequence typing ,epidemiology ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie - Abstract
International audience; The molecular epidemiology of third-generation cephalosporin-resistant (3GC-R) Klebsiella pneumoniae in developing countries is poorly documented. From February 2007 to March 2008, we collected 135 3GC-R K. pneumoniae isolates from seven major towns in Maghreb (Morocco), West Africa (Senegal, Côte d'Ivoire), Central Africa (Cameroon), East Africa (Madagascar) and Southeast Asia (Vietnam). Their genetic diversity, assessed by multilocus sequence typing, was high (60 sequence types), reflecting multiclonality. However, two major clonal groups, CG15 (n = 23, 17% of isolates) and CG258 (n = 18, 13%), were detected in almost all participating centres. The two major clonal groups have previously been described in other parts of the world, indicating their global spread. The high diversity of enterobacterial repetitive intergenic consensus sequence-PCR banding patterns at the local level indicates that most isolates were epidemiologically unrelated. The isolates were characterized by the presence of multiple resistance determinants, most notably the concomitant presence of the aac(6')-Ib-cr, qnr and bla(CTX-M-15) genes in 61 isolates (45%) belonging to 31 sequence types. These isolates were detected across a large geographical area including Cameroon (n = 1), Vietnam (n = 4), Madagascar (n = 10), Côte d'Ivoire (n = 12), Morocco (n = 13) and Senegal (n = 21). These results have major implications for patient management and highlight a potential reservoir for resistance determinants.
- Published
- 2012
19. Screening of Volunteer Students in Yaounde (Cameroon, Central Africa) for Chlamydia trachomatis Infection and Genotyping of Isolated C. trachomatis Strains
- Author
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Françoise Lunel, Jocelyn Thonnon, Antoinette Ngandjio, Friede Njock, Marie Christine Fonkoua, Régis Pouillot, Christiane Bébéar, Bertille de Barbeyrac, M. Clerc, and Anne Bianchi
- Subjects
Microbiology (medical) ,Sexually transmitted disease ,Adult ,Male ,Genotype ,Chlamydiology and Rickettsiology ,Porins ,Chlamydia trachomatis ,medicine.disease_cause ,Risk Factors ,Prevalence ,Medicine ,Humans ,Chlamydiaceae ,Cameroon ,Genotyping ,Chlamydia ,Molecular epidemiology ,biology ,business.industry ,Chlamydia Infections ,biology.organism_classification ,medicine.disease ,Virology ,Chlamydiales ,Female ,business - Abstract
The prevalence of Chlamydia trachomatis infection was 3.78% out of 1,277 volunteer students screened by direct fluorescence assay and Cobas Amplicor PCR. The infection was associated with the nonuse or inconsistent use of condoms in women ( P = 0.026) and a previous sexually transmitted infection in men ( P = 0.023). The most frequent genotypes determined by sequencing the omp1 genes of 25 clinical isolates were E (44%) and F (20%), and some strains harbored mutations, but E genotype strains did not.
- Published
- 2003
20. The Lake Chad Basin, an Isolated and Persistent Reservoir of Vibrio cholerae O1: A Genomic Insight into the Outbreak in Cameroon, 2010.
- Author
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Kaas, Rolf S., Ngandjio, Antoinette, Nzouankeu, Ariane, Siriphap, Achiraya, Fonkoua, Marie-Christine, Aarestrup, Frank M., and Hendriksen, Rene S.
- Subjects
- *
VIBRIO cholerae , *DISEASE prevalence , *DISEASE outbreaks , *MICROBIAL virulence , *SINGLE nucleotide polymorphisms - Abstract
The prevalence of reported cholera was relatively low around the Lake Chad basin until 1991. Since then, cholera outbreaks have been reported every couple of years. The objective of this study was to investigate the 2010/2011 Vibrio cholerae outbreak in Cameroon to gain insight into the genomic make-up of the V. cholerae strains responsible for the outbreak. Twenty-four strains were isolated and whole genome sequenced. Known virulence genes, resistance genes and integrating conjugative element (ICE) elements were identified and annotated. A global phylogeny (378 genomes) was inferred using a single nucleotide polymorphism (SNP) analysis. The Cameroon outbreak was found to be clonal and clustered distant from the other African strains. In addition, a subset of the strains contained a deletion that was found in the ICE element causing less resistance. These results suggest that V. cholerae is endemic in the Lake Chad basin and different from other African strains. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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21. Multidrug-Resistant Salmonella enterica Serotype Typhi, Gulf of Guinea Region, Africa.
- Author
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Baltazar, Murielle, Ngandjio, Antoinette, Holt, Kathryn Elizabeth, Lepillet, Elodie, Pardos de la Gandara, Maria, Collard, Jean-Marc, Bercion, Raymond, Nzouankeu, Ariane, Le Hello, Simon, Dougan, Gordon, Fonkoua, Marie-Christine, and Weill, François-Xavier
- Subjects
- *
MULTIDRUG resistance in bacteria , *SALMONELLA enterica serovar Typhi , *HAPLOTYPES , *PLASMIDS - Abstract
We identified 3 lineages among multidrug-resistant (MDR) Salmonella enterica serotype Typhi isolates in the Gulf of Guinea region in Africa during the 2000s. However, the MDR H58 haplotype, which predominates in southern Asia and Kenya, was not identified. MDR quinolone-susceptible isolates contained a 190-kb incHI1 pST2 plasmid or a 50-kb incN pST3 plasmid. [ABSTRACT FROM AUTHOR]
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- 2015
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22. Prevalence and Antimicrobial Resistance of SalmonellaSerotypes in Chickens from Retail Markets in Yaounde (Cameroon).
- Author
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Marguerite Wouafo, Ariane Nzouankeu, Junie Atangana Kinfack, Marie-Christine Fonkoua, Guy Ejenguele, Thomas Njine, and Antoinette Ngandjio
- Subjects
- *
DISEASE prevalence , *ANTI-infective agents , *DRUG resistance in microorganisms , *SALMONELLA infections in poultry , *CHICKEN industry , *RETAIL industry , *DISEASE susceptibility - Abstract
From February 2006 to January 2007, 150 chickens were collected from eight retail markets in Yaounde, and 90 (60%) tested positive for Salmonella. Seventy-nine chickens were contaminated with only one Salmonellaserotype, 10 with two different serotypes, and 1 with four serotypes. The most prevalent serotypes were Enteritidis (47 strains) and Hadar (29 strains). The isolates were tested for their susceptibilities to amoxicillin, amoxicillin/clavulanic acid, cefoxitin, cefotaxime, gentamicin, streptomycin, tetracycline, chloramphenicol, sulfonamides, nalidixic acid, ciprofloxacin, and trimethoprim/sulfamethazole by disk diffusion assay. Minimum inhibitory concentrations of ampicillin, streptomycin, tetracycline, sulfonamides, and nalidixic acid were determined for the resistant strains by agar dilution method. Eleven isolates (10.7%) of the 103 tested were susceptible to all antimicrobials. Resistance was most observed to tetracycline (84.5%), streptomycin (44.7%), and nalidixic acid (34%). Forty-one isolates (39.8%) were multidrug resistant (resistant to three or more antimicrobials from different classes), of which 68.3% were Hadar and 21.9% Enteritidis. The most frequent resistant pattern in Hadar was streptomycin–tetracycline–nalidixic acid. These results highlight once more the need for surveillance of Salmonellacontamination in poultry. [ABSTRACT FROM AUTHOR]
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- 2010
- Full Text
- View/download PDF
23. Erratum: Global phylogeography and evolutionary history of Shigella dysenteriae type 1.
- Author
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Njamkepo E, Fawal N, Tran-Dien A, Hawkey J, Strockbine N, Jenkins C, Talukder KA, Bercion R, Kuleshov K, Kolínská R, Russell JE, Kaftyreva L, Accou-Demartin M, Karas A, Vandenberg O, Mather AE, Mason CJ, Page AJ, Ramamurthy T, Bizet C, Gamian A, Carle I, Sow AG, Bouchier C, Wester AL, Lejay-Collin M, Fonkoua MC, Le Hello S, Blaser MJ, Jernberg C, Ruckly C, Mérens A, Page AL, Aslett M, Roggentin P, Fruth A, Denamur E, Venkatesan M, Bercovier H, Bodhidatta L, Chiou CS, Clermont D, Colonna B, Egorova S, Pazhani GP, Ezernitchi AV, Guigon G, Harris SR, Izumiya H, Korzeniowska-Kowal A, Lutyńska A, Gouali M, Grimont F, Langendorf C, Marejková M, Peterson LA, Perez-Perez G, Ngandjio A, Podkolzin A, Souche E, Makarova M, Shipulin GA, Ye C, Žemličková H, Herpay M, Grimont PA, Parkhill J, Sansonetti P, Holt KE, Brisse S, Thomson NR, and Weill FX
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- 2016
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24. Multiple contaminations of chickens with Campylobacter, Escherichia coli and Salmonella in Yaounde (Cameroon).
- Author
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Nzouankeu A, Ngandjio A, Ejenguele G, Njine T, and Ndayo Wouafo M
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- Animals, Cameroon, Campylobacter Infections microbiology, Enterobacteriaceae Infections microbiology, Prevalence, Campylobacter Infections veterinary, Campylobacter coli isolation & purification, Campylobacter jejuni isolation & purification, Chickens microbiology, Enterobacteriaceae Infections veterinary, Escherichia coli isolation & purification, Salmonella isolation & purification
- Abstract
Introduction: Food-borne diseases associated with Campylobacter, Escherichia coli, and Salmonella are mainly caused by the consumption of raw or undercooked poultry meat. The objective of this study was to evaluate the prevalence of Campylobacter, Escherichia coli, and Salmonella in chickens., Methodology: One hundred and fifty chickens collected from eight retail markets in Yaounde were examined for the presence of Campylobacter, Escherichia coli, and Salmonella using standard bacteriological procedures., Results: Of the 150 chickens collected, 135 (90%) were contaminated with Campylobacter (68.9% C. coli and 31.1% C. jejuni). All the chickens were positive for E. coli. Among the 150 isolates, 17 (11.3%) were enteropathogenic E. coli (EPEC). Additionally, 103 Salmonella strains were recovered from 90 chickens. Salmonella Enteritidis (45.6%) and Salmonella Hadar (28.1%) were the most frequent serotypes. Multiple contamination was found in 142 chickens (94.6%), of which 83 (55.3%) were concurrently contaminated with Campylobacter, Escherichia coli, and Salmonella., Conclusion: These results show that chickens in Cameroon are highly contaminated with Campylobacter, Escherichia coli, and Salmonella. The multiple contaminations of chickens is a potential risk of infection for consumers and highlights the necessity of public awareness for food safety.
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- 2010
- Full Text
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25. Prevalence and antimicrobial resistance of Salmonella serotypes in chickens from retail markets in Yaounde (Cameroon).
- Author
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Wouafo M, Nzouankeu A, Kinfack JA, Fonkoua MC, Ejenguele G, Njine T, and Ngandjio A
- Subjects
- Animals, Anti-Bacterial Agents pharmacology, Cameroon, Drug Resistance, Multiple, Bacterial, Electrophoresis, Gel, Pulsed-Field methods, Microbial Sensitivity Tests, Prevalence, Salmonella isolation & purification, Serotyping, Chickens microbiology, Drug Resistance, Bacterial, Food Contamination, Meat microbiology, Salmonella classification, Salmonella drug effects
- Abstract
From February 2006 to January 2007, 150 chickens were collected from eight retail markets in Yaounde, and 90 (60%) tested positive for Salmonella. Seventy-nine chickens were contaminated with only one Salmonella serotype, 10 with two different serotypes, and 1 with four serotypes. The most prevalent serotypes were Enteritidis (47 strains) and Hadar (29 strains). The isolates were tested for their susceptibilities to amoxicillin, amoxicillin/clavulanic acid, cefoxitin, cefotaxime, gentamicin, streptomycin, tetracycline, chloramphenicol, sulfonamides, nalidixic acid, ciprofloxacin, and trimethoprim/sulfamethazole by disk diffusion assay. Minimum inhibitory concentrations of ampicillin, streptomycin, tetracycline, sulfonamides, and nalidixic acid were determined for the resistant strains by agar dilution method. Eleven isolates (10.7%) of the 103 tested were susceptible to all antimicrobials. Resistance was most observed to tetracycline (84.5%), streptomycin (44.7%), and nalidixic acid (34%). Forty-one isolates (39.8%) were multidrug resistant (resistant to three or more antimicrobials from different classes), of which 68.3% were Hadar and 21.9% Enteritidis. The most frequent resistant pattern in Hadar was streptomycin-tetracycline-nalidixic acid. These results highlight once more the need for surveillance of Salmonella contamination in poultry.
- Published
- 2010
- Full Text
- View/download PDF
26. Antimicrobial resistance and molecular characterization of Vibrio cholerae O1 during the 2004 and 2005 outbreak of cholera in Cameroon.
- Author
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Ngandjio A, Tejiokem M, Wouafo M, Ndome I, Yonga M, Guenole A, Lemee L, Quilici ML, and Fonkoua MC
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- Cameroon epidemiology, Cholera epidemiology, Colony Count, Microbial, Disease Outbreaks, Electrophoresis, Gel, Pulsed-Field, Feces microbiology, Food Microbiology, Humans, Microbial Sensitivity Tests, Polymerase Chain Reaction, Ribotyping, Vibrio cholerae O1 genetics, Vibrio cholerae O1 isolation & purification, Anti-Bacterial Agents pharmacology, Cholera microbiology, DNA, Bacterial analysis, Drug Resistance, Multiple, Bacterial, Vibrio cholerae O1 drug effects
- Abstract
There was an outbreak of cholera in Cameroon during 2004 and 2005; the epidemic began in Douala in January 2004 and spread throughout the south of the country. The World Health Organization (WHO) reported 8005 cases in 2004 and 2847 cases in 2005. Five hundred eighty-nine stool samples were received in the Pasteur Centre of Cameroon and 352 were microbiologically confirmed to be positive for Vibrio cholerae O1. Isolated strains were tested for their antimicrobial susceptibilities. All the strains were multidrug resistant and predominantly showed a common resistance pattern at the beginning of the outbreak. Tetracycline, recommended by the WHO for treating cholera in adults, was effective against all the strains tested. Cotrimoxazole (trimethoprim/sulfamethoxazole), previously a first-line treatment in children, was ineffective in vitro for all the clinical isolates and was quickly replaced by amoxicillin. Ampicillin resistance emerged at the end of 2004 and was the leading resistance pattern observed in the second half of 2005. This therefore represented the second major resistance pattern. These two major resistance profiles were not associated with patient characteristics (sex and age) or to the geographic origin of strains. However, there was a highly significant relationship between resistance patterns and the year of isolation (p < 0.001). The strains possessed genes ctxA and ctxB encoding the two cholera toxin subunits and were very closely related, irrespective of their antimicrobial resistance patterns. They were not differentiated by molecular typing methods and gave similar ribotyping and pulsed-field gel electrophoresis patterns.
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- 2009
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27. Screening of volunteer students in Yaounde (Cameroon, Central Africa) for Chlamydia trachomatis infection and genotyping of isolated C. trachomatis strains.
- Author
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Ngandjio A, Clerc M, Fonkoua MC, Thonnon J, Njock F, Pouillot R, Lunel F, Bebear C, De Barbeyrac B, and Bianchi A
- Subjects
- Adult, Cameroon epidemiology, Chlamydia Infections diagnosis, Chlamydia Infections etiology, Chlamydia trachomatis isolation & purification, Female, Genotype, Humans, Male, Porins chemistry, Porins genetics, Prevalence, Risk Factors, Chlamydia Infections epidemiology, Chlamydia trachomatis classification
- Abstract
The prevalence of Chlamydia trachomatis infection was 3.78% out of 1,277 volunteer students screened by direct fluorescence assay and Cobas Amplicor PCR. The infection was associated with the nonuse or inconsistent use of condoms in women (P = 0.026) and a previous sexually transmitted infection in men (P = 0.023). The most frequent genotypes determined by sequencing the omp1 genes of 25 clinical isolates were E (44%) and F (20%), and some strains harbored mutations, but E genotype strains did not.
- Published
- 2003
- Full Text
- View/download PDF
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