349 results on '"Ng, Lisa F. P."'
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2. Author Correction: Higher Delta variant-specific neutralizing antibodies prevented infection in close contacts vaccinated with ancestral mRNA vaccines during the SARS-CoV-2 Delta wave
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Goh, Yun Shan, Fong, Siew-Wai, Tay, Matthew Zirui, Rouers, Angeline, Chang, Zi Wei, Chavatte, Jean-Marc, Hor, Pei Xiang, Loh, Chiew Yee, Huang, Yuling, Tan, Yong Jie, Wang, Bei, Ngoh, Eve Zi Xian, Mohd Salleh, Siti Nazihah, Lee, Raphael Tze Chuen, Lim, Georgina, Maurer-Stroh, Sebastian, Wang, Cheng-I, Leo, Yee‐Sin, Lin, Raymond T. P., Lam, Meng Chon, Lye, David C., Young, Barnaby Edward, Ng, Lisa F. P., and Renia, Laurent
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- 2024
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3. Employment of a high throughput functional assay to define the critical factors that influence vaccine induced cross-variant neutralizing antibodies for SARS-CoV-2
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Gu, Yue, Shunmuganathan, Bhuvaneshwari, Qian, Xinlei, Gupta, Rashi, Tan, Rebecca S. W., Kozma, Mary, Purushotorman, Kiren, Murali, Tanusya M., Tan, Nikki Y. J., Preiser, Peter R., Lescar, Julien, Nasir, Haziq, Somani, Jyoti, Tambyah, Paul A., Smith, Kenneth G. C., Renia, Laurent, Ng, Lisa F. P., Lye, David C., Young, Barnaby E., and MacAry, Paul A.
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- 2023
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4. Higher Delta variant-specific neutralizing antibodies prevented infection in close contacts vaccinated with ancestral mRNA vaccines during the SARS-CoV-2 Delta wave
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Goh, Yun Shan, Fong, Siew-Wai, Tay, Matthew Zirui, Rouers, Angeline, Chang, Zi Wei, Chavatte, Jean-Marc, Hor, Pei Xiang, Loh, Chiew Yee, Huang, Yuling, Tan, Yong Jie, Wang, Bei, Ngoh, Eve Zi Xian, Mohd Salleh, Siti Nazihah, Lee, Raphael Tze Chuen, Lim, Georgina, Maurer-Stroh, Sebastian, Wang, Cheng-I, Leo, Yee‐Sin, Lin, Raymond T. P., Lam, Meng Chon, Lye, David C., Young, Barnaby Edward, Ng, Lisa F. P., and Renia, Laurent
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- 2023
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5. Myeloperoxidase inhibition may protect against endothelial glycocalyx shedding induced by COVID-19 plasma
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Teo, Andrew, Chan, Louisa L. Y., Cheung, Christine, Chia, Po Ying, Ong, Sean Wei Xiang, Fong, Siew Wai, Ng, Lisa F. P., Renia, Laurent, Lye, David Chien, Young, Barnaby Edward, and Yeo, Tsin Wen
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- 2023
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6. Author Correction: Chikungunya fever
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Bartholomeeusen, Koen, Daniel, Matthieu, LaBeaud, Desiree A., Gasque, Philippe, Peeling, Rosanna W., Stephenson, Kathryn E., Ng, Lisa F. P., and Ariën, Kevin K.
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- 2023
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7. Chikungunya fever
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Bartholomeeusen, Koen, Daniel, Matthieu, LaBeaud, Desiree A., Gasque, Philippe, Peeling, Rosanna W., Stephenson, Kathryn E., Ng, Lisa F. P., and Ariën, Kevin K.
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- 2023
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8. Monkeypox: disease epidemiology, host immunity and clinical interventions
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Lum, Fok-Moon, Torres-Ruesta, Anthony, Tay, Matthew Z., Lin, Raymond T. P., Lye, David C., Rénia, Laurent, and Ng, Lisa F. P.
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- 2022
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9. Lower vaccine-acquired immunity in the elderly population following two-dose BNT162b2 vaccination is alleviated by a third vaccine dose
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Renia, Laurent, Goh, Yun Shan, Rouers, Angeline, Le Bert, Nina, Chia, Wan Ni, Chavatte, Jean-Marc, Fong, Siew‐Wai, Chang, Zi Wei, Zhuo, Nicole Ziyi, Tay, Matthew Zirui, Chan, Yi-Hao, Tan, Chee Wah, Yeo, Nicholas Kim‐Wah, Amrun, Siti Naqiah, Huang, Yuling, Wong, Joel Xu En, Hor, Pei Xiang, Loh, Chiew Yee, Wang, Bei, Ngoh, Eve Zi Xian, Salleh, Siti Nazihah Mohd, Carissimo, Guillaume, Dowla, Samanzer, Lim, Alicia Jieling, Zhang, Jinyan, Lim, Joey Ming Er, Wang, Cheng-I., Ding, Ying, Pada, Surinder, Sun, Louisa Jin, Somani, Jyoti, Lee, Eng Sing, Ong, Desmond Luan Seng, Leo, Yee‐Sin, MacAry, Paul A., Lin, Raymond Tzer Pin, Wang, Lin-Fa, Ren, Ee Chee, Lye, David C., Bertoletti, Antonio, Young, Barnaby Edward, and Ng, Lisa F. P.
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- 2022
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10. Evaluation of the safety and immunogenicity of different COVID-19 vaccine combinations in healthy individuals: study protocol for a randomized, subject-blinded, controlled phase 3 trial [PRIBIVAC]
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Poh, Xuan Ying, Lee, I. Russel, Lim, Clarissa, Teo, Jefanie, Rao, Suma, Chia, Po Ying, Ong, Sean W. X., Lee, Tau Hong, Lin, Ray J. H., Ng, Lisa F. P., Ren, Ee Chee, Lin, Raymond T. P., Wang, Lin-Fa, Renia, Laurent, Lye, David Chien, and Young, Barnaby E.
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- 2022
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11. Functional and Immunologic Mapping of Domains of the Reticulocyte-Binding Protein Plasmodium vivax PvRBP2a.
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Tay, Matthew Zirui, Tang, Weiyi, Lee, Wenn-Chyau, Ong, Alice Soh Meoy, Novera, Wisna, Malleret, Benoît, Carissimo, Guillaume, Chacko, Ann-Marie, El-Sahili, Abbas, Lescar, Julien, Fan, Yiping, McGready, Rose M, Chu, Cindy S, Chan, Jerry Kok Yen, Ng, Lisa F P, Russell, Bruce, Nosten, François, and Rénia, Laurent
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PLASMODIUM vivax ,MALARIA vaccines ,RETICULOCYTES ,ANTIBODY formation ,LINEAR operators - Abstract
We previously described a novel Plasmodium vivax invasion mechanism into human reticulocytes via the PvRBP2a-CD98 receptor-ligand pair. Using linear epitope mapping, we assessed the PvRBP2a epitopes involved in CD98 binding and recognized by antibodies from patients who were infected. We identified 2 epitope clusters mediating PvRBP2a-CD98 interaction. Cluster B (PvRBP2a
431-448 , TAALKEKGKLLANLYNKL) was the target of antibody responses in humans infected by P vivax. Peptides from each cluster were able to prevent live parasite invasion of human reticulocytes. These results provide new insights for development of a malaria blood-stage vaccine against P vivax. [ABSTRACT FROM AUTHOR]- Published
- 2024
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12. Robust Virus-Specific Adaptive Immunity in COVID-19 Patients with SARS-CoV-2 Δ382 Variant Infection
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Fong, Siew-Wai, Yeo, Nicholas Kim-Wah, Chan, Yi-Hao, Goh, Yun Shan, Amrun, Siti Naqiah, Ang, Nicholas, Rajapakse, Menaka Priyadharsani, Lum, Josephine, Foo, Shihui, Lee, Cheryl Yi-Pin, Carissimo, Guillaume, Chee, Rhonda Sin-Ling, Torres-Ruesta, Anthony, Tay, Matthew Zirui, Chang, Zi Wei, Poh, Chek Meng, Young, Barnaby Edward, Tambyah, Paul A., Kalimuddin, Shirin, Leo, Yee-Sin, Lye, David C., Lee, Bernett, Biswas, Subhra, Howland, Shanshan Wu, Renia, Laurent, and Ng, Lisa F. P.
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- 2022
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13. Coronaviruses in animals and humans
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Ng, Lisa F P and Hiscox, Julian A
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- 2020
14. Plasmodium vivax binds host CD98hc (SLC3A2) to enter immature red blood cells
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Malleret, Benoît, El Sahili, Abbas, Tay, Matthew Zirui, Carissimo, Guillaume, Ong, Alice Soh Meoy, Novera, Wisna, Lin, Jianqing, Suwanarusk, Rossarin, Kosaisavee, Varakorn, Chu, Trang T. T., Sinha, Ameya, Howland, Shanshan Wu, Fan, Yiping, Gruszczyk, Jakub, Tham, Wai-Hong, Colin, Yves, Maurer-Stroh, Sebastian, Snounou, Georges, Ng, Lisa F. P., Chan, Jerry Kok Yen, Chacko, Ann-Marie, Lescar, Julien, Chandramohanadas, Rajesh, Nosten, François, Russell, Bruce, and Rénia, Laurent
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- 2021
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15. Immunity, endothelial injury and complement-induced coagulopathy in COVID-19
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Perico, Luca, Benigni, Ariela, Casiraghi, Federica, Ng, Lisa F. P., Renia, Laurent, and Remuzzi, Giuseppe
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- 2021
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16. Analysis of an Ebola virus disease survivor whose host and viral markers were predictive of death indicates the effectiveness of medical countermeasures and supportive care
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Bosworth, Andrew, Rickett, Natasha Y., Dong, Xiaofeng, Ng, Lisa F. P., García-Dorival, Isabel, Matthews, David A., Fletcher, Tom, Jacobs, Michael, Thomson, Emma C., Carroll, Miles W., and Hiscox, Julian A.
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- 2021
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17. Resistance of SARS-CoV-2 variants to neutralization by convalescent plasma from early COVID-19 outbreak in Singapore
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Wang, Bei, Goh, Yun Shan, Prince, Tessa, Ngoh, Eve Zi Xian, Salleh, Siti Nazihah Mohd, Hor, Pei Xiang, Loh, Chiew Yee, Fong, Siew Wai, Hartley, Catherine, Tan, Seow-Yen, Young, Barnaby Edward, Leo, Yee-Sin, Lye, David C., Maurer-Stroh, Sebastian, Ng, Lisa F. P., Hiscox, Julian A., Renia, Laurent, and Wang, Cheng-I
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- 2021
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18. The trinity of COVID-19: immunity, inflammation and intervention
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Tay, Matthew Zirui, Poh, Chek Meng, Rénia, Laurent, MacAry, Paul A., and Ng, Lisa F. P.
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- 2020
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19. COVID-19 vaccines and kidney disease
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Windpessl, Martin, Bruchfeld, Annette, Anders, Hans-Joachim, Kramer, Holly, Waldman, Meryl, Renia, Laurent, Ng, Lisa F. P., Xing, Zhou, and Kronbichler, Andreas
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- 2021
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20. Correction to: Robust Virus‐Specific Adaptive Immunity in COVID‐19 Patients with SARS‐CoV‐2 Δ382 Variant Infection
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Fong, Siew‐Wai, Yeo, Nicholas Kim‐Wah, Chan, Yi‐Hao, Goh, Yun Shan, Amrun, Siti Naqiah, Ang, Nicholas, Rajapakse, Menaka Priyadharsani, Lum, Josephine, Foo, Shihui, Lee, Cheryl Yi‐Pin, Carissimo, Guillaume, Chee, Rhonda Sin‐Ling, Torres‐Ruesta, Anthony, Tay, Matthew Zirui, Chang, Zi Wei, Poh, Chek Meng, Young, Barnaby Edward, Tambyah, Paul A., Kalimuddin, Shirin, Leo, Yee‐Sin, Lye, David C., Lee, Bernett, Biswas, Subhra, Howland, Shanshan Wu, Renia, Laurent, and Ng, Lisa F. P.
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- 2022
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21. Design and Characterization of a New Formulation for the Delivery of COVID-19-mRNA Vaccine to the Nasal Mucosa.
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Altay Benetti, Ayça, Tan, Eugene Yang Zhi, Chang, Zi Wei, Bae, Ki Hyun, Thwin, Ma Thinzar, Muthuramalingam, Ram Pravin Kumar, Liao, Kuo-Chieh, Wan, Yue, Ng, Lisa F. P., Renia, Laurent, Liu, Jianping, Chen, Xiaoyuan, Yang, Yi Yan, White, Kevin P., and Pastorin, Giorgia
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NASAL mucosa ,SARS-CoV-2 ,EPITHELIAL cells ,DENDRITIC cells ,ANTIBODY formation - Abstract
Chitosan, a natural polysaccharide derived from chitin, possesses biocompatibility, biodegradability, and mucoadhesive characteristics, making it an attractive material for the delivery of mRNA payloads to the nasal mucosa and promoting their uptake by target cells such as epithelial and immune cells (e.g., dendritic cells and macrophages). In this project, we aimed at developing novel lipid-based nanoformulations for mRNA delivery to counteract the pandemic caused by SARS-CoV-2 virus. The formulations achieved a mRNA encapsulation efficiency of ~80.2% with chitosan-lipid nanoparticles, as measured by the RiboGreen assay. Furthermore, the evaluation of SARS-CoV-2 Spike (S) receptor-binding domain (RBD) expression via ELISA for our vaccine formulations showed transfection levels in human embryonic kidney cells (HEK 293), lung carcinoma cells (A549), and dendritic cells (DC 2.4) equal to 9.9 ± 0.1 ng/mL (174.7 ± 1.1 fold change from untreated cells (UT)), 7.0 ± 0.2 ng/mL (128.1 ± 4.9 fold change from UT), and 0.9 ± 0.0 ng/mL (18.0 ± 0.1 fold change from UT), respectively. Our most promising vaccine formulation was also demonstrated to be amenable to lyophilization with minimal degradation of loaded mRNA, paving the way towards a more accessible and stable vaccine. Preliminary in vivo studies in mice were performed to assess the systemic and local immune responses. Nasal bronchoalveolar lavage fluid (BALF) wash showed that utilizing the optimized formulation resulted in local antibody concentrations and did not trigger any systemic antibody response. However, if further improved and developed, it could potentially contribute to the management of COVID-19 through nasopharyngeal immunization strategies. [ABSTRACT FROM AUTHOR]
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- 2024
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22. Longitudinal Study of Cellular and Systemic Cytokine Signatures to Define the Dynamics of a Balanced Immune Environment During Disease Manifestation in Zika Virus–Infected Patients
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Lum, Fok-Moon, Lye, David C. B., Tan, Jeslin J. L., Lee, Bernett, Chia, Po-Ying, Chua, Tze-Kwang, Amrun, Siti N., Kam, Yiu-Wing, Yee, Wearn-Xin, Ling, Wei-Ping, Lim, Vanessa W. X., Pang, Vincent J. X., Lee, Linda K., Mok, Esther W. H., Chong, Chia-Yin, Leo, Yee-Sin, and Ng, Lisa F. P.
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- 2018
23. Specific Biomarkers Associated With Neurological Complications and Congenital Central Nervous System Abnormalities From Zika Virus–Infected Patients in Brazil
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The Zika-Unicamp Network, Kam, Yiu-Wing, Leite, Juliana Almeida, Lum, Fok-Moon, Tan, Jeslin J. L., Lee, Bernett, Judice, Carla C., de Toledo Teixeira, Daniel Augusto, Andreata-Santos, Robert, Vinolo, Marco A., Angerami, Rodrigo, Resende, Mariangela Ribeiro, Freitas, Andre Ricardo Ribas, Amaral, Eliana, Passini, Renato, Costa, Maria Laura, Guida, José Paulo, Arns, Clarice Weis, Ferreira, Luis Carlos S., Rénia, Laurent, Proença-Modena, Jose Luiz, Ng, Lisa F. P., and Costa, Fabio T. M.
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- 2017
24. A Sensitive Method for Detecting Zika Virus Antigen in Patients' Whole-Blood Specimens as an Alternative Diagnostic Approach
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Lum, Fok-Moon, Lin, Cui, Susova, Olga Y., Teo, Teck-Hui, Fong, Siew-Wai, Mak, Tze-Minn, Lee, Linda Kay, Chong, Chia-Yin, Lye, David C. B., Lin, Raymond T. P., Merits, Andres, Leo, Yee-Sin, and Ng, Lisa F. P.
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- 2017
25. Zika Virus Infects Human Fetal Brain Microglia and Induces Inflammation
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Lum, Fok-Moon, Low, Donovan K. S., Fan, Yiping, Tan, Jeslin J. L., Lee, Bernett, Chan, Jerry K. Y., Rénia, Laurent, Ginhoux, Florent, and Ng, Lisa F. P.
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- 2017
26. Severity of Plasma Leakage Is Associated With High Levels of Interferon γ–Inducible Protein 10, Hepatocyte Growth Factor, Matrix Metalloproteinase 2 (MMP-2), and MMP-9 During Dengue Virus Infection
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SlgN Immunomonitoring Platform, Her, Zhisheng, Kam, Yiu-Wing, Gan, Victor C., Lee, Bernett, Thein, Tun-Linn, Tan, Jeslin J. L., Lee, Linda K., Fink, Katja, Lye, David C., Rénia, Laurent, Leo, Yee-Sin, and Ng, Lisa F. P.
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- 2017
27. Chikungunya virus: an update on the biology and pathogenesis of this emerging pathogen
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Burt, Felicity J, Chen, Weiqiang, Miner, Jonathan J, Lenschow, Deborah J, Merits, Andres, Schnettler, Esther, Kohl, Alain, Rudd, Penny A, Taylor, Adam, Herrero, Lara J, Zaid, Ali, Ng, Lisa F P, and Mahalingam, Suresh
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- 2017
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28. Variation around the dominant viral genome sequence contributes to viral load and outcome in patients with Ebola virus disease
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Dong, Xiaofeng, Munoz-Basagoiti, Jordana, Rickett, Natasha Y., Pollakis, Georgios, Paxton, William A., Günther, Stephan, Kerber, Romy, Ng, Lisa F. P., Elmore, Michael J., Magassouba, N’faly, Carroll, Miles W., Matthews, David A., and Hiscox, Julian A.
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- 2020
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29. Two linear epitopes on the SARS-CoV-2 spike protein that elicit neutralising antibodies in COVID-19 patients
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Poh, Chek Meng, Carissimo, Guillaume, Wang, Bei, Amrun, Siti Naqiah, Lee, Cheryl Yi-Pin, Chee, Rhonda Sin-Ling, Fong, Siew-Wai, Yeo, Nicholas Kim-Wah, Lee, Wen-Hsin, Torres-Ruesta, Anthony, Leo, Yee-Sin, Chen, Mark I-Cheng, Tan, Seow-Yen, Chai, Louis Yi Ann, Kalimuddin, Shirin, Kheng, Shirley Seah Gek, Thien, Siew-Yee, Young, Barnaby Edward, Lye, David C., Hanson, Brendon John, Wang, Cheng-I, Renia, Laurent, and Ng, Lisa F. P.
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- 2020
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30. TREM-1 activation is a potential key regulator in driving severe pathogenesis of enterovirus A71 infection
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Amrun, Siti Naqiah, Tan, Jeslin J. L., Rickett, Natasha Y., Cox, Jonathan A., Lee, Bernett, Griffiths, Michael J., Solomon, Tom, Perera, David, Ooi, Mong How, Hiscox, Julian A., and Ng, Lisa F. P.
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- 2020
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31. Rare predicted loss-of-function variants of type I IFN immunity genes are associated with life-threatening COVID-19
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Matuozzo, Daniela, Talouarn, Estelle, Marchal, Astrid, Zhang, Peng, Manry, Jeremy, Seeleuthner, Yoann, Zhang, Yu, Bolze, Alexandre, Chaldebas, Matthieu, Milisavljevic, Baptiste, Gervais, Adrian, Bastard, Paul, Asano, Takaki, Bizien, Lucy, Barzaghi, Federica, Abolhassani, Hassan, Abou Tayoun, Ahmad, Aiuti, Alessandro, Alavi Darazam, Ilad, Allende, Luis M., Alonso-Arias, Rebeca, Arias, Andrés Augusto, Aytekin, Gokhan, Bergman, Peter, Bondesan, Simone, Bryceson, Yenan T., Bustos, Ingrid G., Cabrera-Marante, Oscar, Carcel, Sheila, Carrera, Paola, Casari, Giorgio, Chaïbi, Khalil, Colobran, Roger, Condino-Neto, Antonio, Covill, Laura E., Delmonte, Ottavia M., El Zein, Loubna, Flores, Carlos, Gregersen, Peter K., Gut, Marta, Haerynck, Filomeen, Halwani, Rabih, Hancerli, Selda, Hammarström, Lennart, Hatipoğlu, Nevin, Karbuz, Adem, Keles, Sevgi, Kyheng, Christèle, Leon-Lopez, Rafael, Franco, Jose Luis, Mansouri, Davood, Martinez-Picado, Javier, Metin Akcan, Ozge, Migeotte, Isabelle, Morange, Pierre-Emmanuel, Morelle, Guillaume, Martin-Nalda, Andrea, Novelli, Giuseppe, Novelli, Antonio, Ozcelik, Tayfun, Palabiyik, Figen, Pan-Hammarström, Qiang, de Diego, Rebeca Pérez, Planas-Serra, Laura, Pleguezuelo, Daniel E., Prando, Carolina, Pujol, Aurora, Reyes, Luis Felipe, Rivière, Jacques G., Rodriguez-Gallego, Carlos, Rojas, Julian, Rovere-Querini, Patrizia, Schlüter, Agatha, Shahrooei, Mohammad, Sobh, Ali, Soler-Palacin, Pere, Tandjaoui-Lambiotte, Yacine, Tipu, Imran, Tresoldi, Cristina, Troya, Jesus, van de Beek, Diederik, Zatz, Mayana, Zawadzki, Pawel, Al-Muhsen, Saleh Zaid, Alosaimi, Mohammed Faraj, Alsohime, Fahad M., Baris-Feldman, Hagit, Butte, Manish J., Constantinescu, Stefan N., Cooper, Megan A., Dalgard, Clifton L., Fellay, Jacques, Heath, James R., Lau, Yu-Lung, Lifton, Richard P., Maniatis, Tom, Mogensen, Trine H., von Bernuth, Horst, Lermine, Alban, Vidaud, Michel, Boland, Anne, Deleuze, Jean-François, Nussbaum, Robert, Kahn-Kirby, Amanda, Mentre, France, Tubiana, Sarah, Gorochov, Guy, Tubach, Florence, Hausfater, Pierre, Abel, Laurent, Al-Muhsen, Saleh, Al-Mulla, Fahd, Anderson, Mark S., Andreakos, Evangelos, Arias, Andrés A., Feldman, Hagit Baris, Belot, Alexandre, Biggs, Catherine M., Bogunovic, Dusan, Bondarenko, Anastasiia, Bousfiha, Ahmed A., Brodin, Petter, Bryceson, Yenan, Bustamante, Carlos D., Chakravorty, Samya, Christodoulou, John, Desai, Murkesh, Drolet, Beth A., Baghdadi, Jamila El, Espinosa-Padilla, Sara, Franco, José Luis, Froidure, Antoine, Hagin, David, Henrickson, Sarah E., Hsieh, Elena W. Y., Husebye, Eystein, Imai, Kohsuke, Itan, Yuval, Jarvis, Erich D., Karamitros, Timokratis, Kisand, Kai, Ku, Cheng-Lung, Ling, Yun, Lucas, Carrie L., Maródi, László, Meyts, Isabelle, Milner, Joshua D., Mironska, Kristina, Morio, Tomohiro, Ng, Lisa F. P., Notarangelo, Luigi D., O’Farrelly, Cliona, Okada, Satoshi, Planas, Anna M., Quintana-Murci, Lluis, Renia, Laurent, Resnick, Igor, Rodríguez-Gallego, Carlos, Sancho-Shimizu, Vanessa, Sediva, Anna, Seppänen, Mikko R. J., Shcherbina, Anna, Slaby, Ondrej, Snow, Andrew L., Soler-Palacín, Pere, Spaan, András N., Tancevski, Ivan, Tangye, Stuart G., Ramaswamy, Sathishkumar, Turvey, Stuart E., Uddin, Furkan, Uddin, Mohammed J., Vinh, Donald C., Su, Helen C., Casanova, Jean-Laurent, Bureau, Serge, Vacher, Yannick, Gysembergh-Houal, Anne, Demerville, Lauren, Chachoua, Abla, Abad, Sebastien, Abassi, Radhiya, Abdellaoui, Abdelrafie, Abdelmalek, Abdelkrim, Abdoul, Hendy, Abergel, Helene, Abeud, Fariza, Abgrall, Sophie, Abisror, Noemie, Adechian, Marylise, Aderdour, Nordine, Admane, Hakeem Farid, Adnet, Frederic, Afritt, Sara, Agostini, Helene, Aguilar, Claire, Agut, Sophie, Aiello, Tommaso Francesco, Kaci, Marc Ait, Oufella, Hafid Ait, Ajeenthiravasan, Gokula, Alauzy, Virginie, Alby-Laurent, Fanny, Allard, Lucie, Alyanakian, Marie-Alexandra, Borrero, Blanca Amador, Amam, Sabrina, Amrouche, Lucile, Andronikof, Marc, Anglicheau, Dany, Anguel, Nadia, Annane, Djillali, Aounzou, Mohammed, Aparicio, Caroline, Aratus, Gladys, Arlet, Jean-Benoit, Arzoine, Jeremy, Aslangul, Elisabeth, Assefi, Mona, Aubry, Adeline, Audiffred, Laetitia, Audureau, Etienne, Auger, Christelle Nathalie, Auregan, Jean-Charles, Awotar, Celine, Milla, Sonia Ayllon, Azan, Delphine, Azemar, Laurene, Azzouguen, Billal, Elrufaai, Marwa Bachir, Badsi, Aïda, Bakouboula, Prissile, Balcerowiak, Coline, Balde, Fanta, Baldivia, Elodie, Bangamingo, Eliane-Flore, Baptiste, Amandine, Baran-Marszak, Fanny, Barau, Caroline, Barget, Nathalie, Baronnet, Flore, Barthelemy, Romain, Baudel, Jean-Luc, Baudry, Camille, Baudry, Elodie, Beaugerie, Laurent, Belamri, Adel, Belaube, Nicolas, Belilita, Rhida, Bellassen, Pierre, Belmokhtar, Rawan, Beltran, Isabel, Benainous, Ruben, Benallaoua, Mourad, Benamouzig, Robert, Benbara, Amélie, Benhida, Jaouad, Benkhelouf, Anis, Benlagha, Jihene, Benmostafa, Chahinez, Benothmane, Skander, Bentifraouine, Miassa, Berard, Laurence, Bernier, Quentin, Berti, Enora, Bertier, Astrid, Berton, Laure, Bessis, Simon, Beurton, Alexandra, Bianco, Celine, Bianquis, Clara, Bidar, Frank, Blanche, Philippe, Blayau, Clarisse, Bleibtreu, Alexandre, Blin, Emmanuelle, Bloch-Queyrat, Coralie, Boissier, Marie-Christophe, Bollens, Diane, Bolzoni, Marion, Bompard, Rudy pierre, Bonnet, Nicolas, Bonnouvrier, Justine, Botha, Shirmonecrystal, Boucenna, Wissam, Bouchama, Fatiha, Bouchaud, Olivier, Bouchghoul, Hanane, Boudjebla, Taoueslylia, Boudjema, Noel, Bouffard, Catherine, Bougle, Adrien, Bouguerra, Meriem, Bouras, Leila, Bourcier, Agnes, Durand, Anne Bourgarit, Bourrier, Anne, Bouscarat, Fabrice, Bouvry, Diane, Bouziri, Nesrine, Bouzrara, Ons, Bribier, Sarah, Brugier, Delphine, Brunel, Melanie, Bui, Eida, Buisson, Anne, Bukreyeva, Iryna, Bureau, Côme, Cadranel, Jacques, Cailhol, Johann, Calin, Ruxandra, Vega, Clara Campos, Canavaggio, Pauline, Cancella, Marta, Cantin, Delphine, Cao, Albert, Carbillon, Lionel, Carlier, Nicolas, Cassard, Clementine, Castor, Guylaine, Cauchy, Marion, Cha, Olivier, Chaigne, 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Gonzalo, Okamoto, Keisuke, Oualha, Mehdi, Ouedrani, Amani, Özçelik, Tayfun, Ozkaya-Parlakay, Aslinur, Pagani, Michele, Papadaki, Maria, Parola, Philippe, Pascreau, Tiffany, Paul, Stéphane, Paz-Artal, Estela, Pedraza, Sigifredo, Pellecer, Nancy Carolina González, Pellegrini, Silvia, Pérez-Fernández, Xosé Luis, Philippe, Aurélien, Philippot, Quentin, Picod, Adrien, de Chambrun, Marc Pineton, Piralla, Antonio, Ploin, Dominique, Poissy, Julien, Poncelet, Géraldine, Poulakou, Garyphallia, Pouletty, Marie S., Pourshahnazari, Persia, Qiu-Chen, Jia Li, Quentric, Paul, Rambaud, Thomas, Raoult, Didier, Raoult, Violette, Rebillat, Anne-Sophie, Redin, Claire, Resmini, Léa, Ricart, Pilar, Richard, Jean-Christophe, Rigo-Bonnin, Raúl, rivet, Nadia, Rocamora-Blanch, Gemma, Rodero, Mathieu P., Rodrigo, Carlos, Rodriguez, Luis Antonio, Rodriguez-Palmero, Agustí, Romero, Carolina Soledad, Rothenbuhler, Anya, Roux, Damien, Rovina, Nikoletta, Rozenberg, Flore, Ruch, Yvon, Ruiz, Montse, del Prado, Maria Yolanda Ruiz, Ruiz-Rodriguez, Juan Carlos, Sabater-Riera, Joan, Saks, Kai, Salagianni, Maria, Sanchez, Oliver, Sánchez-Montalvá, Adrián, Sánchez-Ramón, Silvia, Schidlowski, Laire, Schluter, Agatha, Schmidt, Julien, Schmidt, Matthieu, Schuetz, Catharina, Schweitzer, Cyril E., Scolari, Francesco, Seijo, Luis, Seminario, Analia Gisela, Seng, Piseth, Senoglu, Sevtap, Seppänen, Mikko, Llovich, Alex Serra, Siguret, Virginie, Siouti, Eleni, Smadja, David M., Smith, Nikaia, Solanich, Xavier, Solé-Violán, Jordi, Soler, Catherine, Sözeri, Betül, Stella, Giulia Maria, Stepanovskiy, Yuriy, Stoclin, Annabelle, Taccone, Fabio, Taupin, Jean-Luc, Tavernier, Simon, Tello, Loreto Vidaur, Terrier, Benjamin, Thiery, Guillaume, Thorball, Christian, Thorn, Karolina, Thumerelle, Caroline, Tolstrup, Martin, Tomasoni, Gabriele, Toubiana, Julie, Alvarez, Josep Trenado, Triantafyllia, Vasiliki, Trouillet-Assant, Sophie, Troya, Jesús, Tsang, Owen T. Y., Tserel, Liina, Tso, Eugene Y. K., Tucci, Alessandra, Öz, Şadiye Kübra Tüter, Ursini, Matilde Valeria, Utsumi, Takanori, Vabres, Pierre, Valencia-Ramos, Juan, Van Den Rym, Ana Maria, Vandernoot, Isabelle, Velez-Santamaria, Valentina, Veliz, Silvia Patricia Zuniga, Vidigal, Mateus C., Viel, Sébastien, Villain, Cédric, Vilaire-Meunier, Marie E., Villar-García, Judit, Vincent, Audrey, Volokha, Alla, Vuotto, Fanny, Wauters, Els, Wauters, Joost, Wu, Alan K. L., Wu, Tak-Chiu, Yahşi, Aysun, Yesilbas, Osman, Yildiz, Mehmet, Young, Barnaby E., Yükselmiş, Ufuk, Zecca, Marco, Zuccaro, Valentina, Van Praet, Jens, Lambrecht, Bart, Van Braeckel, Eva, Bosteels, Cedric, Hoste, Levi, Hoste, Eric, Bauters, Fre, Heijmans, Catherine, Slabbynck, Hans, Naesens, Leslie, Florkin, Benoit, Boulanger, Cécile, Vanderlinden, Dimitri, Berkell, Matilda, Carelli, Valerio, Fiorentino, Alessio, Malhotra, Surbi, Mattiaccio, Alessandro, Pippucci, Tommaso, Seri, Marco, Tacconelli, Evelina, van Agtmael, Michiel, Algera, Anne Geke, Appelman, Brent, van Baarle, Frank, Bax, Diane, Beudel, Martijn, Bogaard, Harm Jan, Bomers, Marije, Bonta, Peter, Bos, Lieuwe, Botta, Michela, de Brabander, Justin, de Bree, Godelieve, de Bruin, Sanne, Buis, David T. P., Bugiani, Marianna, Bulle, Esther, Cloherty, Osoul Chouchane Alex, Dijkstra, Mirjam, Dongelmans, Dave A., Dujardin, Romein W. G., Elbers, Paul, Fleuren, Lucas, Geijtenbeek, Suzanne Geerlings Theo, Girbes, Armand, Goorhuis, Bram, Grobusch, Martin P., Hafkamp, Florianne, Hagens, Laura, Hamann, Jorg, Harris, Vanessa, Hemke, Robert, Heunks, Sabine M. Hermans Leo, Hollmann, Markus, Horn, Janneke, Hovius, Joppe W., de Jong, Menno D., Koning, Rutger, Lim, Endry H. T., van Mourik, Niels, Nellen, Jeaninne, Nossent, Esther J., Paulus, Frederique, Peters, Edgar, Pina-Fuentes, Dan A. I., van der Poll, Tom, Preckel, Bennedikt, Prins, Jan M., Raasveld, Jorinde, Reijnders, Tom, de Rotte, Maurits C. F. J., Schinkel, Michiel, Schultz, Marcus J., Schrauwen, Femke A. P., Schuurmans, Alex, Schuurmans, Jaap, Sigaloff, Kim, Slim, Marleen A., Smeele, Patrick, Smit, Marry, Stijnis, Cornelis S., Stilma, Willemke, Teunissen, Charlotte, Thoral, Patrick, Tsonas, Anissa M., Tuinman, Pieter R., van der Valk, Marc, Veelo, Denise, Volleman, Carolien, de Vries, Heder, Vught, Lonneke A., van Vugt, Michèle, Wouters, Dorien, Zwinderman, A. H., Brouwer, Matthijs C., Wiersinga, W. Joost, Vlaar, Alexander P. J., Tompkins, Miranda F., Alba, Camille, Hupalo, Daniel N., Rosenberger, John, Sukumar, Gauthaman, Wilkerson, Matthew D., Zhang, Xijun, Lack, Justin, Oler, Andrew J., Dobbs, Kerry, Danielson, Jeffrey J., Biondi, Andrea, Bettini, Laura Rachele, D’Angio’, Mariella, Beretta, Ilaria, Imberti, Luisa, Sottini, Alessandra, Quaresima, Virginia, Quiros-Roldan, Eugenia, Rossi, Camillo, Zhang, Shen-Ying, Declercq, Jozefien, Puel, Anne, Boisson-Dupuis, Stephanie, Boisson, Bertrand, Jouanguy, Emmanuelle, Zhang, Qian, Cobat, Aurélie, COVID Human Genetic Effort, [missing], COVIDeF Study Group, [missing], French COVID Cohort Study Group, [missing], CoV-Contact Cohort, [missing], COVID-STORM Clinicians, [missing], COVID Clinicians, [missing], Orchestra Working Group, [missing], Amsterdam UMC Covid-19 Biobank, [missing], and NIAID-USUHS COVID Study Group, [missing]
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Medicine and Health Sciences ,Genetics ,Molecular Medicine ,Molecular Biology ,Genetics (clinical) - Published
- 2023
32. Specific inhibition of NLRP3 in chikungunya disease reveals a role for inflammasomes in alphavirus-induced inflammation
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Chen, Weiqiang, Foo, Suan-Sin, Zaid, Ali, Teng, Terk-Shin, Herrero, Lara J., Wolf, Stefan, Tharmarajah, Kothila, Vu, Luan D., van Vreden, Caryn, Taylor, Adam, Freitas, Joseph R., Li, Rachel W., Woodruff, Trent M., Gordon, Richard, Ojcius, David M., Nakaya, Helder I., Kanneganti, Thirumala-Devi, O’Neill, Luke A. J., Robertson, Avril A. B., King, Nicholas J., Suhrbier, Andreas, Cooper, Matthew A., Ng, Lisa F. P., and Mahalingam, Suresh
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- 2017
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33. A Systematic Meta-analysis of Immune Signatures in Patients With Acute Chikungunya Virus Infection
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Teng, Terk-Shin, Kam, Yiu-Wing, Lee, Bernett, Hapuarachchi, Hapuarachchige Chanditha, Wimal, Abeyewickreme, Ng, Lee-Ching, and Ng, Lisa F. P.
- Published
- 2015
34. Decoding the Human Genetic and Immunological Basis of COVID-19 mRNA Vaccine-Induced Myocarditis
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Bolze, Alexandre, Mogensen, Trine H., Zhang, Shen-Ying, Abel, Laurent, Andreakos, Evangelos, Arkin, Lisa M., Borghesi, Alessandro, Brodin, Petter, Hagin, David, Novelli, Giuseppe, Okada, Satoshi, Peter, Jonny, Renia, Laurent, Severe, Karine, Tiberghien, Pierre, Vinh, Donald C., Aiuti, Alessandro, Al-Muhsen, Saleh, Al-Mulla, Fahd, Amara, Ali, Anderson, Mark S., Arias, Andrés A., Feldman, Hagit Baris, Bastard, Paul, Belot, Alexandre, Biggs, Catherine M., Bogunovic, Dusan, Bousfiha, Ahmed A., Butte, Manish J., Christodoulou, John, Cobat, Aurelie, Colobran, Roger, Condino-Neto, Antonio, Constantinescu, Stefan N., Dalgard, Clifton L., Duval, Xavier, Eloy, Philippine, Espinosa-Padilla, Sara, Fellay, Jacques, Flores, Carlos, Franco, José Luis, Froidure, Antoine, Gorochov, Guy, Gregersen, Peter K., Haerynck, Filomeen, Halwani, Rabih, Hammarström, Lennart, Itan, Yuval, Jouanguy, Emmanuelle, Karamitros, Timokratis, Lau, Yu-Lung, Mansouri, Davood, Mentre, France, Meyts, Isabelle, Mironska, Kristina, Morio, Tomohiro, Ng, Lisa F. P., Novelli, Antonio, O’Farrelly, Cliona, Okamoto, Keisuke, Ozcelik, Tayfun, Pan-Hammarström, Qiang, de Diego, Rebeca Perez, Perez-Tur, Jordi, Perlin, David S., Pesole, Graziano, Planas, Anna M., Prando, Carolina, Pujol, Aurora, Quintana-Murci, Lluis, Resnick, Igor, Rodríguez-Gallego, Carlos, Sancho-Shimizu, Vanessa, Sediva, Anna, Seppänen, Mikko R. J., Shahrooei, Mohammed, Shcherbina, Anna, Slaby, Ondrej, Soler-Palacín, Pere, Soumelis, Vassili, Spaan, András N., Tancevski, Ivan, Tangye, Stuart G., Tayoun, Ahmad Abou, Temel, Şehime G. lsün, Thorball, Christian, Trouillet-Assant, Sophie, Turvey, Stuart E., Uddin, K. MFurkan, van de Beek, Diederik, von Bernuth, Horst, Zhang, Qian, Cirulli, Elizabeth T., Casanova, Jean-Laurent, Hsieh, Elena W. Y., Neurology, AII - Infectious diseases, and ANS - Neuroinfection & -inflammation
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Myocarditis ,COVID-19 Vaccines ,Settore MED/03 ,Vaccination ,Immunology ,Humans ,COVID-19 ,Immunology and Allergy ,Human Genetics ,IMUNOGENICIDADE DA VACINA - Abstract
More than 10 billion doses of COVID-19 vaccines have been administered worldwide in the span of 18 months, providing an unprecedented opportunity to study and understand immunological responses and clinical reactions to vaccines. While severe adverse reactions to live-attenuated viral or bacterial vaccines have been successfully deciphered since the 1950s, with the discovery of a wide range of underlying inborn errors of immunity [1, 2], there is currently no molecular, cellular, and immunological explanation for life-threatening reactions to any other type of vaccine. COVID-19 mRNA vaccines are very effective at preventing hypoxemic COVID-19 pneumonia. For example, their efficacy for preventing invasive mechanical ventilation and in-hospital death has been estimated at 90% (95% CI = 88–91%) [3]. COVID-19 mRNA vaccines are also well tolerated by most people, with either no side effects or mild local/systemic reactions, such as pain at the injection site, fever, or chills in the days following vaccination [4]. However, rare but serious adverse events have been observed, including anaphylaxis, myocarditis, Guillain-Barré syndrome, transverse myelitis, Bell’s palsy, and multisystem inflammatory syndrome [4,5,6,7]. These adverse events have a combined prevalence of about 90 per million doses administered [4]. It is unknown whether they are triggered by the adjuvant (lipid nanoparticles for the mRNA vaccines), the vaccine antigen (the perfusion-stabilized spike protein translated by the human cells), the core components of the vaccine (the nucleoside-modified mRNA), or a combination thereof. Some possible mechanisms have been suggested [8,9,10], but the underlying immunopathology and, hence, the risk factors predisposing a minority of vaccinees to experience any of these severe reactions remain unknown.
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- 2022
35. Fingolimod treatment abrogates chikungunya virus–induced arthralgia
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Teo, Teck-Hui, Chan, Yi-Hao, Lee, Wendy W. L., Lum, Fok-Moon, Amrun, Siti Naqiah, Her, Zhisheng, Rajarethinam, Ravisankar, Merits, Andres, Rötzschke, Olaf, Rénia, Laurent, and Ng, Lisa F. P.
- Published
- 2017
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36. Age has a role in driving host immunopathological response to alphavirus infection
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Chan, Yi‐Hao and Ng, Lisa F. P.
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- 2017
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- View/download PDF
37. Multimodal assessments of Zika virus immune pathophysiological responses in marmosets
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Lum, Fok-Moon, Zhang, Wei, Lim, Kheng-Choon, Malleret, Benoit, Teo, Teck-Hui, Koh, Jun-Jia, Lee, Kuan J., Chua, Tze-Kwang, Kam, Yiu-Wing, Yee, Wearn-Xin, Huen, Isaac, Tan, Jeslin J. L., Amrun, Siti Naqiah, Prakash KN, Bhanu, Cozzone, Patrick J., Renia, Laurent, Lee, Philip T. H., and Ng, Lisa F. P.
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- 2018
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- View/download PDF
38. Plasmodium co-infection protects against chikungunya virus-induced pathologies
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Teo, Teck-Hui, Lum, Fok-Moon, Ghaffar, Khairunnisa, Chan, Yi-Hao, Amrun, Siti Naqiah, Tan, Jeslin J. L., Lee, Cheryl Y. P., Chua, Tze-Kwang, Carissimo, Guillaume, Lee, Wendy W. L., Claser, Carla, Rajarethinam, Ravisankar, Rénia, Laurent, and Ng, Lisa F. P.
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- 2018
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- View/download PDF
39. Recessive inborn errors of type I IFN immunity in children with COVID-19 pneumonia
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Zhang, Qian, Matuozzo, Daniela, Le Pen, Jérémie, Lee, Danyel, Moens, Leen, Asano, Takaki, Bohlen, Jonathan, Liu, Zhiyong, Moncada-Velez, Marcela, Kendir-Demirkol, Yasemin, Jing, Huie, Bizien, Lucy, Marchal, Astrid, Abolhassani, Hassan, Delafontaine, Selket, Bucciol, Giorgia, COVID Human Genetic Effort, Bayhan, Gulsum Ical, Keles, Sevgi, Kiykim, Ayca, Hancerli, Selda, Haerynck, Filomeen, Florkin, Benoit, Hatipoglu, Nevin, Ozcelik, Tayfun, Morelle, Guillaume, Zatz, Mayana, Ng, Lisa F P, Lye, David Chien, Young, Barnaby Edward, Leo, Yee-Sin, Dalgard, Clifton L, Lifton, Richard P, Renia, Laurent, Meyts, Isabelle, Jouanguy, Emmanuelle, Hammarström, Lennart, Pan-Hammarström, Qiang, Boisson, Bertrand, Bastard, Paul, Su, Helen C, Boisson-Dupuis, Stéphanie, Abel, Laurent, Rice, Charles M, Zhang, Shen-Ying, Cobat, Aurélie, Casanova, Jean-Laurent, Froidure, Antoine, School of Biological Sciences, Lee Kong Chian School of Medicine (LKCMedicine), A*STAR Infectious Diseases Labs, National Centre for Infectious Diseases, National University of Singapore, Tan Tock Seng Hospital, UCL - SSS/IREC/PNEU - Pôle de Pneumologie, ORL et Dermatologie, AII - Infectious diseases, Amsterdam Neuroscience - Neuroinfection & -inflammation, Neurology, and Özçelik, Tayfun
- Subjects
Adult ,SARS-CoV-2 ,Immunology ,Inheritance Patterns ,COVID-19 ,Pneumonia ,COVID-19/genetics ,Simplex-Virus Encephalitis ,Settore MED/03 ,Interferon Type I ,Pyogenic Bacterial-Infections ,Medicine and Health Sciences ,Immunology and Allergy ,Humans ,Medicine [Science] ,Child - Abstract
Recessive or dominant inborn errors of type I interferon (IFN) immunity can underlie critical COVID-19 pneumonia in unvaccinated adults. The risk of COVID-19 pneumonia in unvaccinated children, which is much lower than in unvaccinated adults, remains unexplained. In an international cohort of 112 children (
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- 2022
40. Prolonged inflammation in patients hospitalized for coronavirus disease 2019 (COVID‐19) resolves 2 years after infection.
- Author
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Fong, Siew‐Wai, Goh, Yun Shan, Torres‐Ruesta, Anthony, Chang, Zi Wei, Chan, Yi‐Hao, Neo, Vanessa Kexin, Lee, Bernett, Duan, Kaibo, Amrun, Siti Naqiah, Yeo, Nicholas Kim‐Wah, Chen, Hsiuyi V., Tay, Matthew Zirui, Carissimo, Guillaume, Tan, Seow Yen, Leo, Yee‐Sin, Lye, David C., Renia, Laurent, Young, Barnaby Edward, and Ng, Lisa F. P.
- Subjects
SARS-CoV-2 ,COVID-19 ,CORONAVIRUS diseases - Abstract
Long‐term complications from coronavirus disease 2019 (COVID‐19) are concerning, as survivors can develop subclinical multiorgan dysfunction. It is unknown if such complications are due to prolonged inflammation, and severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) vaccination may reduce sequela. We conducted a prospective longitudinal study on hospitalized patients over 24 months. Clinical symptoms were collected by self‐reporting during follow‐up, along with blood samples for quantification of inflammatory markers and immune cell frequencies. All patients were given one dose of mRNA vaccine at 12−16 months. Their immune profiles at 12 and 24 months were compared. Approximately 37% and 39% of our patients reported post‐COVID‐19 symptoms at 12 and 24 months, respectively. The proportion of symptomatic patients with more than one symptom decreased from 69% at 12 months to 56% at 24 months. Longitudinal cytokine profiling revealed a cluster of individuals with persistently high inflammatory cytokine levels 12 months after infection. Patients with prolonged inflammation showed elevated terminally differentiated memory T cells in their blood; 54% had symptoms at 12 months. The majority of inflammatory markers and dysregulated immune cells in vaccinated patients recovered to a healthy baseline at 24 months, even though symptoms persisted. Post‐COVID‐19 symptoms can linger for 2 years after the initial infection and are associated with prolonged inflammation. Prolonged inflammation in hospitalized patients resolves after 2 years. We define a set of analytes associated with persistent inflammation and presence of symptoms, which could be useful biomarkers for identifying and monitoring high‐risk survivors. [ABSTRACT FROM AUTHOR]
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- 2023
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- View/download PDF
41. Reply to Noret et al
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Her, Zhisheng, Lum, Fok-Moon, Chow, Angela, Leo, Yee-Sin, Rénia, Laurent, Chiocchia, Gilles, and Ng, Lisa F. P.
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- 2012
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- View/download PDF
42. Early Appearance of Neutralizing Immunoglobulin G3 Antibodies Is Associated With Chikungunya Virus Clearance and Long-term Clinical Protection
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Kam, Yiu-Wing, Simarmata, Diane, Chow, Angela, Her, Zhisheng, Teng, Terk-Shin, Ong, Edward K. S., Rénia, Laurent, Leo, Yee-Sin, and Ng, Lisa F. P.
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- 2012
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43. Antibody Response of Heterologous vs Homologous Messenger RNA Vaccine Boosters Against the Severe Acute Respiratory Syndrome Coronavirus 2 Omicron Variant: Interim Results from the PRIBIVAC Study, a Randomized Clinical Trial.
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Poh, Xuan Ying, Tan, Chee Wah, Lee, I Russel, Chavatte, Jean-Marc, Fong, Siew-Wai, Prince, Tessa, Hartley, Catherine, Yeoh, Aileen Y Y, Rao, Suma, Chia, Po Ying, Ong, Sean W X, Lee, Tau Hong, Sadarangani, Sapna P, Lin, Ray J H, Lim, Clarissa, Teo, Jefanie, Lim, Daniel R X, Chia, Wanni, Hiscox, Julian A, and Ng, Lisa F P
- Subjects
STATISTICAL significance ,SARS-CoV-2 ,COVID-19 ,IMMUNIZATION ,SCIENTIFIC observation ,CONFIDENCE intervals ,COVID-19 vaccines ,MULTIPLE regression analysis ,IMMUNOCOMPROMISED patients ,FISHER exact test ,MANN Whitney U Test ,ANTIBODY formation ,RANDOMIZED controlled trials ,T-test (Statistics) ,MATHEMATICAL variables ,MESSENGER RNA ,DESCRIPTIVE statistics ,CHI-squared test ,NEUTRALIZATION tests ,VIRAL antibodies ,STATISTICAL sampling ,DATA analysis software ,IMMUNOSUPPRESSIVE agents ,LONGITUDINAL method - Abstract
Background Waning antibody levels post-vaccination and the emergence of variants of concern (VOCs) capable of evading protective immunity have raised the need for booster vaccinations. However, which combination of coronavirus disease 2019 (COVID-19) vaccines offers the strongest immune response against the Omicron variant is unknown. Methods This randomized, participant-blinded, controlled trial assessed the reactogenicity and immunogenicity of different COVID-19 vaccine booster combinations. A total of 100 BNT162b2-vaccinated individuals were enrolled and randomized 1:1 to either homologous (BNT162b2 + BNT162b2 + BNT162b2; "BBB") or heterologous messenger RNA (mRNA) (BNT162b2 + BNT162b2 + mRNA-1273; "BBM") booster vaccine. The primary end point was the level of neutralizing antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) wild-type and VOCs at day 28. Results A total of 51 participants were allocated to BBB and 49 to BBM; 50 and 48, respectively, were analyzed for safety and immunogenicity outcomes. At day 28 post-boost, mean SARS-CoV-2 spike antibody titers were lower with BBB (22 382 IU/mL; 95% confidence interval [CI], 18 210 to 27 517) vs BBM (29 751 IU/mL; 95% CI, 25 281 to 35 011; P =.034) as was the median level of neutralizing antibodies: BBB 99.0% (interquartile range [IQR], 97.9% to 99.3%) vs BBM 99.3% (IQR, 98.8% to 99.5%; P =.021). On subgroup analysis, significant higher mean spike antibody titer, median surrogate neutralizing antibody level against all VOCs, and live Omicron neutralization titer were observed only in older adults receiving BBM. Both vaccines were well tolerated. Conclusions Heterologous mRNA-1273 booster vaccination compared with homologous BNT123b2 induced a stronger neutralizing response against the Omicron variant in older individuals. Clinical Trials Registration NCT05142319. [ABSTRACT FROM AUTHOR]
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- 2022
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44. DECADE IN REVIEW—CNS INFECTIONS: Major advances against a moving target of CNS infections
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Ng, Lisa F. P. and Solomon, Tom
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- 2015
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45. Conserved longitudinal alterations of anti-S-protein IgG subclasses in disease progression in initial ancestral Wuhan and vaccine breakthrough Delta infections.
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Yun Shan Goh, Siew-Wai Fong, Pei Xiang Hor, Amrun, Siti Naqiah, Cheryl Yi-Pin Lee, Young, Barnaby Edward, Po Ying Chia, Tambyah, Paul A., Kalimuddin, Shirin, Pada, Surinder, Seow-Yen Tan, Jin Sun, Louisa, Chen, Mark I.-Cheng, Yee-Sin Leo, Lye, David C., Ng, Lisa F. P., and Renia, Laurent
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BREAKTHROUGH infections ,DISEASE progression ,SARS-CoV-2 Delta variant ,IMMUNOSPECIFICITY ,COVID-19 vaccines ,IMMUNOGLOBULINS ,IMMUNOGLOBULIN G ,IMMUNOGLOBULIN M - Abstract
Introduction: COVID-19 has a wide disease spectrum ranging from asymptomatic to severe. While humoral immune responses are critical in preventing infection, the immune mechanisms leading to severe disease, and the identification of biomarkers of disease progression and/or resolution of the infection remains to be determined. Methods: Plasma samples were obtained from infections during the initial wave of ancestral wildtype SARS-CoV-2 and from vaccine breakthrough infections during the wave of Delta variant, up to six months post infection. The spike-specific antibody profiles were compared across different severity groups and timepoints. Results: We found an association between spike-specific IgM, IgA and IgG and disease severity in unvaccinated infected individuals. In addition to strong IgG1 and IgG3 response, patients with severe disease develop a robust IgG2 and IgG4 response. A comparison of the ratio of IgG1 and IgG3 to IgG2 and IgG4 showed that disease progression is associated with a smaller ratio in both the initial wave of WT and the vaccine breakthrough Delta infections. Time-course analysis revealed that smaller (IgG1 and IgG3)/(IgG2 and IgG4) ratio is associated with disease progression, while the reverse associates with clinical recovery. Discussion: While each IgG subclass is associated with disease severity, the balance within the four IgG subclasses may affect disease outcome. Acute disease progression or infection resolution is associated with a specific immunological phenotype that is conserved in both the initial wave of WT and the vaccine breakthrough Delta infections. [ABSTRACT FROM AUTHOR]
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- 2022
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46. Mouse models for Chikungunya virus: deciphering immune mechanisms responsible for disease and pathology
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Teo, Teck-Hui, Lum, Fok-Moon, Lee, Wendy W. L., and Ng, Lisa F. P.
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- 2012
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47. Discrepant serological findings in SARS‐CoV‐2 PCR‐negative hospitalized patients with fever and acute respiratory symptoms during the pandemic.
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Cross, Gail B., Naftalin, Claire M., Ngiam, Jinghao N., Bagdasarian, Natasha, Poh, Chek M., Goh, Yun S., Chia, Wan N., Amrun, Siti N., Tham, Sai M., Teng, Hazel, Alagha, Rawan, Kumar, Shoban K., Tan, Shaun S. Y., Wang, Lin F., Tambyah, Paul A., Renia, Laurent, Fisher, Dale, and Ng, Lisa F. P.
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SARS-CoV-2 ,HOSPITAL patients ,CORONAVIRUS diseases ,COVID-19 - Abstract
Coronavirus Disease 2019 (COVID‐19) serology has an evolving role in the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection. However, its use in hospitalized patients with acute respiratory symptoms remains unclear. Hospitalized patients with acute respiratory illness admitted to an isolation ward were recruited. All patients had negative nasopharyngeal swab polymerase chain reaction (PCR) for SARS‐CoV‐2. Serological studies using four separate assays (cPass: surrogate neutralizing enzyme‐linked immunosorbent assay [ELISA]; Elecsys: N‐antigen based chemiluminescent assay; SFB: S protein flow‐based; epitope peptide‐based ELISA) were performed on stored plasma collected from patients during the initial hospital stay, and a convalescent visit 4–12 weeks later. Of the 51 patients studied (aged 54, interquartile range 21–84; 62.7% male), no patients tested positive on the Elecsys or cPass assays. Out of 51 patients, 5 had antibodies detected on B‐cell Epitope Assay and 3/51 had antibodies detected on SFB assay. These 8 patients with positive serological test to COVID‐19 were more likely to have a high‐risk occupation (p = 0.039), bacterial infection (p = 0.028), and neutrophilia (p = 0.013) during their initial hospital admission. Discrepant COVID‐19 serological findings were observed among those with recent hospital admissions and bacterial infections. The positive serological findings within our cohort raise important questions about the interpretation of sero‐epidemiology during the current pandemic. [ABSTRACT FROM AUTHOR]
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- 2022
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- View/download PDF
48. Catching bird flu in a droplet
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Pipper, Juergen, Inoue, Masafumi, Ng, Lisa F-P, Neuzil, Pavel, Zhang, Yi, and Novak, Lukas
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- 2007
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49. Viral dynamics and immune correlates of COVID-19 disease severity
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Young, Barnaby E, Ong, Sean W X, Ng, Lisa F P, Anderson, Danielle E, Chia, Wan Ni, Chia, Po Ying, Ang, Li Wei, Mak, Tze-Minn, Kalimuddin, Shirin, Chai, Louis Yi Ann, Pada, Surinder, Tan, Seow Yen, Sun, Louisa, Parthasarathy, Purnima, Fong, Siew-Wai, Chan, Yi-Hao, Tan, Chee Wah, Lee, Bernett, Rötzschke, Olaf, Ding, Ying, Tambyah, Paul, Low, Jenny G H, Cui, Lin, Barkham, Timothy, Lin, Raymond Tzer Pin, Leo, Yee-Sin, Renia, Laurent, Wang, Lin-Fa, and Lye, David Chien
- Subjects
immunology ,AcademicSubjects/MED00290 ,Severe acute respiratory syndrome-related coronavirus ,SARS-CoV-2 ,Major Article ,viral culture ,COVID-19 ,serology ,Humans ,Antibodies, Viral ,cytokines - Abstract
Background Key knowledge gaps remain in the understanding of viral dynamics and immune response of SARS-CoV-2 infection. Methods We evaluated these characteristics and established their association with clinical severity in a prospective observational cohort study of 100 patients with PCR-confirmed SARS-CoV-2 infection (mean age 46 years, 56% male, 38% with comorbidities). Respiratory samples (n=74) were collected for viral culture, serum samples for measurement of IgM/IgG levels (n=30), and plasma samples for levels of inflammatory cytokines and chemokines (n=81). Disease severity was correlated with results from viral culture, serologic testing, and immune markers. Results 57 (57%) patients developed viral pneumonia, of whom 20 (20%) required supplemental oxygen including 12 (12%) invasive mechanical ventilation. Viral culture from respiratory samples was positive for 19 of 74 patients (26%). No virus was isolated when the PCR cycle threshold (Ct) value was >30 or >14 days after symptom onset. Seroconversion occurred at a median of 12.5 days (IQR 9-18) for IgM and 15.0 days (IQR 12-20) for IgG; 54/62 patients (87.1%) sampled at day 14 or later seroconverted. Severe infections were associated with earlier seroconversion and higher peak IgM and IgG levels. Levels of IP-10, HGF, IL-6, MCP-1, MIP-1α, IL-12p70, IL-18, VEGF-A, PDGF-BB and IL-1RA significantly correlated with disease severity. Conclusion We found virus viability was associated with lower PCR Ct value in early illness. A stronger antibody response was associated with disease severity. The overactive proinflammatory immune signatures offers targets for host-directed immunotherapy which should be evaluated in randomised controlled trials.
- Published
- 2020
50. Macrophage Migration Inhibitory Factor Receptor CD74 Mediates Alphavirus-Induced Arthritis and Myositis in Murine Models of Alphavirus Infection
- Author
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Herrero, Lara J., Sheng, Kuo-Ching, Jian, Peng, Taylor, Adam, Her, Zhisheng, Herring, Belinda L., Chow, Angela, Leo, Yee-Sin, Hickey, Michael J., Morand, Eric F., Ng, Lisa F. P., Bucala, Richard, and Mahalingam, Suresh
- Published
- 2013
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