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Plasma medicine is a multidisciplinary field of science that uses cold atmospheric pressure plasmas for biomedical purposes. Several applications have been proposed in the last years, including disinfection of bacteria and fungi, wound healing, dermatological diseases and selective killing of cancer cells. Several plasma production schemes and plasma source designs have been proposed, most of which have obtained interesting therapeutic effects on biological samples. However, an overall understanding of the mechanisms that induce such benefices is still missing. Such an understanding is not trivial, considering the multiplicity of possible interactions between complex systems, such as cold plasmas and biological tissues.

Among human and animal diseases agents, parasites are those that show, with very few exceptions, an environmental resistance far superior with respect other pathogens [1]. This is due to the complex structure of the wall of their infective stages, that makes them particularly resistant to almost all physical and chemical agents. In this work, a novel technique to inactivate coccidian parasites in liquid environment has been investigated and implemented. A Petri dish filled with 20 ml of water contaminated by Eimeria spp. oocysts has been placed within a plasma reactor constituted by a lower HV exposed electrode and an upper grounded copper strip electrode covered by a ceramic slab (Fig. 1). The reactor has been supplied with sinusoidal and nanosecond-pulsed voltage waveforms varying the application time. In both cases an atmospheric pressure Dielectric Barrier Discharge (DBD) has been ignited in the air gap above the liquid surface and in contact with it [2]. The temperature of the solution and its pH level were not significantly changed by plasma treatment. The concentration of ozone and hydrogen peroxide increase by increasing treatment time [2]. Plasma treatment efficacy has been detected considering a representative population of 100 oocysts. For both discharges, treatment efficacy has been found to be dose dependent (Fig. 2). After an energy dose treatment of about 100 kJ/l, the number of sporulated oocytes are roughly halved with respect the control sample. Results are statistically significant with a p

INTRODUCTION. The use of plasma for sanitization purpose is of increasing interest. Annular plasma synthetic jet actuators (PSJAs) had demonstrated their ability to produce and induced tubular flow (Neretti et al., 2017, J. Phys. D: Appl. Phys. 50 015210, 9pp) normal to the surface where the dielectric barrier discharge is ignited. This typology of reactor enhances the transport of reactive species toward the treated samples (Taglioli et al., 2016, Plasma sources Sci. Technol., 25 06LT01-5pp.). Long life charged particles are generated within the plasma region and then can be advected together with the induced flow. In this work the effect of free charges into induced flow have been tested against Candida guilliermondii both in agar plates and in water suspension. MATERIALS AND METHODS. Eighty ml suspension of C. guiliiermondii in saline solution (0.5 Mcfarland opacity) was prepared. The CFU of untreated suspension was determined performing multiple dilution that were plated on Sabouraud Dextrose Agar (SDA) petri dishes that were incubated at 26°C for 48 hours. Aliquots of 100 μl of suspension were spread on the surface of 6 SDA plates (9 cm diameter); aliquots of 20 ml of suspension were put into six 9 cm plates. Agar plates were subjected to induced flow for 30 sec, 2 and 5 minutes and suspension plates for 5, 10 and 15 minutes, both with and without blocking the charges by a grounded metallic mesh. After treating, from each suspension CFU was calculated as previous described, while in the agar plates the colonies were directly counted after incubation. RESULTS AND CONCLUSIONS. The untreated yeast suspension contained 5.21 x 105 CFU/100 μl. In treated samples, the reduction of the CFU of the yeast was related to the treatment time and to presence/absence of free charges. The effect was greater on agar plate than on suspension: at the same time of treatment (5 minutes), only 1-log reduction of CFU were observed on suspension, while 4-log reduction was observed in agar plates. Additional 1-log reduction was observed when free charges have been allowed to reach the sample. On suspension, longer treatment time (15 min) was needed to obtain 2-log reduction without free charges and 4-log reduction with free charges.

This paper deals with the design of an experiment devoted to MHD flow control, to be performed in CIRA GHIBLI hypersonic facility. The criteria utilized to define the proper test conditions and to design and realize the model are here described. The expansion in the nozzle has been investigated through numerical simulations in order to have a preliminary estimate of the ionization degree and of the heat fluxes over the test article. Moreover, an MFD code has been used in order to calculate the expected interaction effect, under the assumption of low Rem. The same procedure has been utilized for the design of an MHD test to be performed in the CIRA SCIROCCO facility.

Atmospheric-pressure plasma treatments for industrial and biomedical applications are often performed using Dielectric Barrier Discharge reactors. Dedicated power supplies are needed to provide the high voltage frequency waveforms to operate these nonlinear and time-dependent loads. Moreover, there is a growing technical need for reliable and reproducible treatments, which require the discharge parameters to be actively controlled. In this work, we illustrate a low-cost power supply topology based on a push-pull converter. We perform experimental measurements on two different reactor topologies (surface and volumetric), showing that open loop operation of the power supply leads to a temperature and average power increase over time. The temperature increases by Delta T-vol similar to 120 degrees C and Delta T-sup similar to 70 degrees C, while the power increases by Delta P-vol similar to 78% and Delta P-sup similar to 60% for the volumetric (40 s) and superficial reactors (120 s), respectively. We discuss how these changes are often unwanted in practical applications. A simplified circuital model of the power supply-reactor system is used to infer the physical relation between the observed reactor thermal behavior and its electrical characteristics. We then show a control strategy for the power supply voltage to ensure constant average power operation of the device based on real-time power measurements on the high voltage side of the power supply and an empirical expression relating the delivered power to the power supply output voltage. These are performed with an Arduino Due microcontroller unit, also used to control the power supply. In a controlled operation the measured power stays within 5% of the reference value for both configurations, reducing the temperature increments to Delta T-vol similar to 80 degrees C and Delta T-sup similar to 44 degrees C, respectively. The obtained results show that the proposed novel control strategy is capable of following the transient temperature behavior, achieving a constant average power operation and subsequently limiting the reactor thermal stress.

This paper presents recent activities covering different plasma fields, from both theoretical and experimental point of views. An overview of the present interests of the scientific community is reported here. Starting from a brief description of the role of collisions in astrophysical plasmas, some fundamental aspects of gas discharges modelling, such as superelastic collisions and the basic concept of vibrational temperature, are discussed. Different plasma sources, as DBD and microwave discharges with their own specific applications, are reported. Edge plasmas in nuclear fusion reactors are investigated, focusing on the cooling mechanisms resulting from nitrogen puffing in the divertor region. Graphic abstract: [Figure not available: see fulltext.].

In this paper, a multi-stage numerical methodology for the description of the Dielectric Barrier Discharge physics in air is discussed. The behavior of the heavy species is computed using drift-diffusion equations. Electrons are taken into account by solving a non-linear formulation of electrostatics. The physical effects of the steamer discharges are modelled by means of a simplified 0D approach. The model also includes a semi-implicit 0D model for the assessment of the elementary chemical processes occurring in air. The developed methodology is employed for the simulation of a volumetric Dielectric Barrier Discharge reactor. The obtained species number density and surface charge deposition rates and are shown and discussed.

In this paper a high-voltage sinusoidal power supply controlled by Arduino DUE micro-controller is described. This generator can feed a dielectric barrier discharge (DBD) load with sinusoidal voltages up to 20 kV peak and frequencies in the range 10&ndash, 60 kHz, with a maximum output power of 200 W. Output voltage can be produced either in a continuous mode, or with on/off modulation cycles, according to treatment/application requirements. This power source is equipped with on-board diagnostics used to measure the output voltage and the charge delivered to the load. With a sample frequency of 500 kHz, Arduino DUE allows to evaluate both the high voltage and the average power feeding the discharge without the use of an expensive external measurement setup. Lissajous techniques are utilized to calculate discharge average power in a quasi-real-time manner. When a load is connected to high-voltage terminals, a self-tuning procedure is carried out to obtain the best working frequency. This parameter allows to minimize power-electronic component stress and to maximize generator efficiency.

To investigate ozone yield limit in low temperature plasmas, a detailed thermodynamic model is developed to calculate theoretical ozone yield for the first time. Theoretical ozone yield is calculated both from overall reaction and detailed reactions. In the former case, the highest theoretical ozone yield of 1211±2 g kWh−1 is obtained when final gas temperature equals the initial one, and all energy is effectively utilized to synthesize ozone. When final gas temperature is not equal to the initial one, theoretical ozone yield increases with the increase of oxygen admixture ratio and oxygen conversion ratio as well as the decrease of final gas temperature. Theoretical ozone yields are 921.22 g kWh−1 and 487.54 g kWh−1 in pure oxygen and in synthetic air respectively at final gas temperature of 400 K and oxygen conversion ratio of 10%. When detailed reactions and electron energy distribution function is considered, theoretical ozone yield rapidly increases by enhancing reduced field. Oxygen admixture ratio also has non-negligible effects on ozone yield. A higher oxygen admixture ratio leads to higher energy efficiency. The theoretical ozone yields are 238.92 g kWh−1 and 191.14 g kWh−1 in pure oxygen and in synthetic air at reduced field of 300 Td respectively. Graphical abstract: [Figure not available: see fulltext.].

In this study we examine both the effect of changing the applied voltage waveform shape and the modulation on-time on the amount of ozone dissolved within a liquid in a dielectric barrier discharges (DBD) bubble reactor. In this device, the discharge forms at the gas–liquid interface allowing for effective transfer of the plasma effluent into the liquid. To produce different voltage waveforms, a multilevel inverter power supply capable of generating arbitrary waveforms without switching-on and switching-off transients has been used. Of the four waveforms used in the study (sinusoidal, sawtooth, square and short-pulse), the square waveform was found to be the most efficient at producing the highest ozone concentration for a fixed peak voltage and average power. To determine the effect of the modulation on-time, the number of cycles during the on-time were increased from 1 up to 1000, adjusting the off-time accordingly to maintain the same duty cycle. Shorter on-time periods were found to be more efficient. Experimental and computational results indicate that the time between subsequent discharges is critical for increased ozone generation efficacy, as this needs to be long enough for ozone produced in one discharge event to diffuse away from the discharge region before the next discharge event occurs, thereby avoiding its partial destruction in the plasma. This insight provides a valuable criterion for the optimization of DBD reactors used in novel biomedical, agricultural and environmental applications.

This paper reports the characterization of a low-power, atmospheric pressure plasma source developed for and successfully tested in applications of plasma medicine. The source produces plasma by ionizing a helium flow containing traces of air. The effluent gas is thus enriched in reactive oxygen and nitrogen species as well as helium metastable excited states. Electrical measurements, Schlieren imaging, OES and various chemical analyses and probes enabled us to fully characterize the plasma source and to determine some fundamental parameters for treating living samples such as the power feeding the discharge, the optimal source to target distance, the maximum exposure time, and to identify the major chemical species responsible for the biological action on exposed living matter.

Annular plasma synthetic jet actuators demonstrated their ability to produce a tubular flow normal to the surface where the dielectric barrier discharge (DBD) is ignited. These fluid-dynamic actuators enhance the delivery of reactive species towards the target to be treated. In these actuators, long life charged particles are generated within the plasma region and then carried on by the induced flow. In this work, the potential distribution induced by charges deposited over an insulating target has been measured. Surface DBD actuators, made with different dielectric materials, have been supplied by different sinusoidal voltages at a constant average power supplying the discharge. Actuators with the exposed electrode connected to both the high voltage potential and grounded have been tested. The charge distribution accumulated on a target surface perpendicular to the flow has been measured at different time intervals with the plasma on. Charges advected by the flow are always positive. The potential distribution on the target surface generated by the charges has been measured. For both configurations, firstly an M-shaped distribution develops and, later on, it becomes bell-shaped. A charge build-up mechanism has been found to be faster when the exposed electrode is connected to the high voltage terminal. The target has been placed at a variable distance from 1 to 5 cm from the actuator surface. At a distance of 5 cm, induced potentials are two times smaller than those with the target at 1 cm. Measurements allow us to estimate a charge flux toward the target on the order of 1011 particles (cm2 s)−1. The results presented in this work show that the presence of charged particles in the jet flow outside the plasma could be an important factor to be accounted for when these plasma actuators are used for treatment purposes (indirect plasma treatment).

The electrohydrodynamic (EHD) interaction induced in atmospheric air pressure by a surface dielectric barrier discharge (DBD) actuator has been experimentally investigated. Plasma synthetic jet actuators (PSJAs) are DBD actuators able to induce an air stream perpendicular to the actuator surface. These devices can be used in the field of aerodynamics to prevent or induce flow separation, modify the laminar to turbulent transition inside the boundary layer, and stabilize or mix air flows. They can also be used to enhance indirect plasma treatment effects, increasing the reactive species delivery rate onto surfaces and liquids. This can play a major role in plasma processing and chemical kinetics modelling, where often only diffusive mechanisms are considered. This paper reports on the importance that different electrode geometries can have on the performance of different PSJAs. A series of DBD aerodynamic actuators designed to produce perpendicular jets has been fabricated on two-layer printed circuit boards (PCBs). Both linear and annular geometries were considered, testing different upper electrode distances in the linear case and different diameters in the annular one. An AC voltage supplied at a peak of 11.5 kV and a frequency of 5 kHz was used. Lower electrodes were connected to the ground and buried in epoxy resin to avoid undesired plasma generation on the lower actuator surface. Voltage and current measurements were carried out to evaluate the active power delivered to the discharges. Schlieren imaging allowed the induced jets to be visualized and gave an estimate of their evolution and geometry. Pitot tube measurements were performed to obtain the velocity profiles of the PSJAs and to estimate the mechanical power delivered to the fluid. The optimal values of the inter-electrode distance and diameter were found in order to maximize jet velocity, mechanical power or efficiency. Annular geometries were found to achieve the best performance.

This work reports an experimental investigation performed in wind tunnel facility on a NACA0015 airfoil equipped with a set of vectorizable fluid-dynamic DBD plasma actuators. The ability of the actuators to recover the stall condition with free stream velocities in the range 5-23 m/s has been investigated. The discharge has been generated by feeding the actuators with a sinusoidal voltage waveform with a frequency of 15 kHz and voltages up to 7.5 kV peak. The actuator positioned on the leading edge exhibits highest ability in the stall recovery. Unsteady actuation obtained by alternatively switching on and off the discharge is more effective in the stall recovery when compared with a steady actuation. The frequency that optimizes the stall recovery has been found to be a function of the velocity with the power of 1.5. This result leads to the determination of a constant Strouhal number if the boundary layer thickness is used as characteristic length. Finally the lift coefficient, obtained when the plasma actuator has been turned on, has been found to be a linear function of the applied voltage.

An advanced flow control experiment, concerning magnetohydrodynamics (MHD) interaction over a blunt body, has been designed by Alta, CIRA and Department of Electrical Engineering (DIE) of University of Bologna and to be realized in CIRA's plasma wind tunnel "Scirocco" under a technology research program (TRP) funded by the European Space Agency (ESA). This work is a further step of the researches performed during the last decade by Alta and DIE in the field of MHD interaction. This experimental campaign foresees the employment of a 50 kW electromagnet, enclosed into an insulated blunt-shaped metallic TPS convectively cooled and electrically insulated by a ceramic coating. The test article has been designed for an air flow with nominal stagnation conditions of p0=2 bar and h0=22 MJ/kg, but several other conditions are planned to be investigated. The aim of the experimental campaign, along with a better understanding of the physical phenomena occurring within an highly ionized shock layer with an applied magnetic field, is the comparison of the collected data with the numerical predictions of CFD codes developed by CIRA. © 2011 by the American Institute of Aeronautics and Astronautics, Inc. All rights reserved.