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We studied the possibility of evaluation of the intensity of pathological oxidative processes in rat liver using an integral bioluminescent test with controlled perfusion of the isolated organ. The test revealed a significant correlation between the level of TBA-reactive products and bioluminescence intensity.

ATP activity in mouse bone marrow cells was studied in in vitro experiments. ATP in physiological and supraphysiological concentrations stimulated blebbing of the plasma membrane. Subacute administration of doxorubicin (inductor of oxidative stress) decreased cell sensitivity to ATP, which corresponded to changes in membrane lipid peroxidation.

Studies by the method of splenic exocolonies with serotonin receptor blockers methysergide and cyproheptadine showed that S2 receptors are involved in the stimulatory effect of serotonin on hemopoietic bone marrow stem cells.

We studied in vitro cytostatic effect of doxorubicin on bone marrow cells preincubated with NAD and reduced glutathione. The presence of NAD and glutathione in the medium potentiated membrane toxicity of this antibiotic, but prevented its toxic effects on intracellular pool of pyridine nucleotides during oxidative stress.

We studied the effects of cis-dichlorodiammine platinum (II) on different pathways of cell death in cultured Ehrlich ascites carcinoma in vitro and on subsequent growth of transplanted tumor in vivo. One-hour incubation with the cytostatic modulated apoptosis in cell culture. However, exposure of cell culture to a minimal effective concentration of cis-platinum(II)diammine dichloride promoted the growth of transplanted tumor.

The metabolic state of rat liver was studied in vivo 1, 6, and 18 h after single hyperthermia (42 degrees C). It was shown that intracellular compensatory reactions involved in restoration of the energy state and realization of endogenous cytoprotective mechanisms play a role during recovery.