1,079 results on '"Nebulization"'
Search Results
2. Effect of Nebulized and Intravenous Hypertonic Saline 3% on the Management of Patients With Acute Respiratory Distress Syndrome
- Author
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Mohamed Elsayed Mohamed Elfakhrany, Resident of Anesthesiology, Surgical Intensive Care and Pain Medicine, Faculty of Medicine, Tanta University, Tanta, Egypt.
- Published
- 2024
3. Aerosolized Antibiotic Therapy in Mechanically Ventilated Patients.
- Author
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Ehrmann, Stephan, Barbier, François, and Li, Jie
- Subjects
VENTILATOR-associated pneumonia ,LUNG infections ,CRITICALLY ill ,INTUBATION ,PHARMACOKINETICS - Abstract
Delivering antibiotics directly to the respiratory tract through inhalation to address lung infections has garnered clinical and scientific interest for decades, given the potential favorable pharmacokinetic profile of this administration route. Among critically ill patients, the burden of healthcare-associated pulmonary infections particularly drove continued interest in delivering inhaled antibiotics to intubated patients. We present a concise overview of the existing rationale and evidence and provide guidance for implementing inhaled antibiotics among ventilated critically ill patients, emphasizing insights from recent literature. [ABSTRACT FROM AUTHOR]
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- 2024
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4. The Effects of Nebulized Lidocaine on the Laryngeal Adductor Reflex.
- Author
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Sánchez Roldán, M. Ángeles, Duque, Héctor, Masso, Bernat, Moncho, Dulce, Vilallonga, Ramón, Armengol, Manuel, and González, Óscar
- Abstract
The laryngeal adductor reflex (LAR) is a brainstem reflex that closes the vocal fold and constitutes a new method for continuously monitoring the vagus and laryngeal nerves during different surgeries. Previous reports concluded that topical lidocaine in spray inhibited LAR responses. However, topical anesthesia in the upper airway may be necessary in awake intubation. We present six patients who underwent neck endocrine surgery due to an intrathoracic goiter that compromised the airway. Before awake intubation, a nebulization of lidocaine 5% was applied for at least 10 min. The intubation procedure was well tolerated, and bilateral LAR with suitable amplitudes for monitoring was obtained in all cases. In our series, the nebulization of lidocaine 5% did not affect the laryngeal adductor reflex. Laryngoscope, 134:4161–4164, 2024 [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
5. Comparison of nebulization with lignocaine and dexamethasone for attenuation of post-operative sore throat: A randomized controlled trial.
- Author
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Saini, Rahul, Johar, Sanjay, Deora, Rahul, Yadav, Pritam, Gehlaut, Preeti, and Kumar, Prashant
- Subjects
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RANDOMIZED controlled trials , *LIDOCAINE , *DEXAMETHASONE , *PATIENT satisfaction , *THROAT - Abstract
Background: Post-operative sore throat (POST) is a very common anesthesia-related event which may hamper patient satisfaction and increase treatment cost. Aims and Objectives: This study aimed to compare the effectiveness of nebulization with dexamethasone and lignocaine to prevent POST. Materials and Methods: This randomized controlled study involved 135 patients randomly divided into three equal groups: Group D - (n=45) received 8 mg (2 mL) dexamethasone plus 3 mL of distilled water, Group L - (n=45) received 80 mg (4% lignocaine 2 mL) plus 3 mL distilled, and Group S - (n=45) received nebulization with 5 mL normal saline. Results: At 4 h postoperatively, the incidence of POST was 15.6% in the dexamethasone group, 33.3% in the lignocaine group, and 73.3% in the saline group. The score was significantly higher in the saline group (P=0.001) compared to the dexamethasone and lignocaine group, while it was comparable between dexamethasone and lignocaine group (P=0.188). Similarly, dexamethasone and lignocaine groups were comparable at immediately post-operative, 2, 8, 12, and 24 h post-operative time points. Post-operative hoarseness scores of all three groups were comparable at all-time points. A significant increase in the heart rate (HR) and mean arterial pressure (MAP) after intubation was observed in the saline and dexamethasone group as compared to the lignocaine group (P=0.001) while HR and MAP were comparable in dexamethasone and saline group (P=1.000). Conclusion: Dexamethasone and lignocaine nebulization are both effective and comparable prophylaxis for POST and lignocaine nebulization has added advantage of blunting pressor response to endotracheal intubation over dexamethasone nebulization. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
6. Prevalence and Management of Chronic Obstructive Pulmonary Disease in the Gulf Countries with a Focus on Inhaled Pharmacotherapy.
- Author
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Tashkin, Donald P., Barjaktarevic, Igor, Gomez-Seco, Julio, Behbehani, Naser Hassan, Koltun, Arkady, and Siddiqui, Urooj Alam
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CHRONIC obstructive pulmonary disease , *METERED-dose inhalers , *SMOKING , *AIR pollutants , *OLDER patients - Abstract
Background: Chronic obstructive pulmonary disease (COPD) is a preventable, progressive disease and the third leading cause of death worldwide. The epidemiological data of COPD from Gulf countries are very limited, as it remains underdiagnosed and underestimated. Risk factors for COPD include tobacco cigarette smoking, water pipe smoking (Shisha), exposure to air pollutants, occupational dusts, fumes, and chemicals. Inadequate treatment of COPD leads to worsening of disease. The 2024 GOLD guidelines recommend use of inhaled bronchodilators, corticosteroids, and adjunct therapies for treatment and management of COPD patients based on an individual assessment of the severity of symptoms and risk of exacerbations. This article reviews COPD pharmacotherapy in the Gulf countries and explores the role of nebulization in the management of COPD in this region. Methods: To review the COPD pharmacotherapy in the Gulf Countries, literature search was conducted using PubMed, Medline, Cochrane Systematic Reviews, and Google Scholar databases (before December 2022), using search terms such as COPD, nebulization, inhalers/inhalation, aerosols, and Gulf countries. Relevant articles from the reference list of identified studies were reviewed. Consensus statements, expert opinion, and other published review articles were included. Results: In the Gulf countries, pressurized metered-dose inhalers (pMDIs), dry powder inhalers (DPIs), soft mist inhalers, and nebulizers are used for drug delivery to COPD patients. pMDIs and DPIs are most prone to errors in technique and other common device handling errors. Nebulization is another mode of inhalation drug delivery, which is beneficial in certain patient populations such as the elderly and patients with cognitive impairment, motor or neuromuscular disorders, and other comorbidities. Conclusion: There is no major difference between Gulf countries and rest of the world in the approach to management of COPD. Nebulizers should be considered for patients who have difficulties in accessing or using MDIs and DPIs, irrespective of geographical location. [ABSTRACT FROM AUTHOR]
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- 2024
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7. Nebulized Glutathione as a Key Antioxidant for the Treatment of Oxidative Stress in Neurodegenerative Conditions.
- Author
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Lana, João Vitor, Rios, Alexandre, Takeyama, Renata, Santos, Napoliane, Pires, Luyddy, Santos, Gabriel Silva, Rodrigues, Izair Jefthé, Jeyaraman, Madhan, Purita, Joseph, and Lana, Jose Fábio
- Abstract
Glutathione (GSH), a tripeptide synthesized intracellularly, serves as a pivotal antioxidant, neutralizing reactive oxygen species (ROS) and reactive nitrogen species (RNS) while maintaining redox homeostasis and detoxifying xenobiotics. Its potent antioxidant properties, particularly attributed to the sulfhydryl group (-SH) in cysteine, are crucial for cellular health across various organelles. The glutathione-glutathione disulfide (GSH-GSSG) cycle is facilitated by enzymes like glutathione peroxidase (GPx) and glutathione reductase (GR), thus aiding in detoxification processes and mitigating oxidative damage and inflammation. Mitochondria, being primary sources of reactive oxygen species, benefit significantly from GSH, which regulates metal homeostasis and supports autophagy, apoptosis, and ferroptosis, playing a fundamental role in neuroprotection. The vulnerability of the brain to oxidative stress underscores the importance of GSH in neurological disorders and regenerative medicine. Nebulization of glutathione presents a novel and promising approach to delivering this antioxidant directly to the central nervous system (CNS), potentially enhancing its bioavailability and therapeutic efficacy. This method may offer significant advantages in mitigating neurodegeneration by enhancing nuclear factor erythroid 2-related factor 2 (NRF2) pathway signaling and mitochondrial function, thereby providing direct neuroprotection. By addressing oxidative stress and its detrimental effects on neuronal health, nebulized GSH could play a crucial role in managing and potentially ameliorating conditions such as Parkinson's Disease (PD) and Alzheimer's Disease (AD). Further clinical research is warranted to elucidate the therapeutic potential of nebulized GSH in preserving mitochondrial health, enhancing CNS function, and combating neurodegenerative conditions, aiming to improve outcomes for individuals affected by brain diseases characterized by oxidative stress and neuroinflammation. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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8. Solid Lipid Nanoparticles Loaded with Dexamethasone Palmitate for Pulmonary Inflammation Treatment by Nebulization Approach.
- Author
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Chen, Hsin-Hung, Sang, Chen-Hsiang, Chou, Chang-Wei, Lin, Yi-Ting, Chang, Yi-Shou, and Chiu, Hsin-Cheng
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ALVEOLAR macrophages , *COLLOIDAL stability , *NANOPARTICLES , *LUNGS , *INFECTION control - Abstract
Pneumonia stands as the leading infectious cause of childhood mortality annually, underscoring its significant impact on pediatric health. Although dexamethasone (DXMS) is effective for treating pulmonary inflammation, its therapeutic potential is compromised by systemic side effects and suboptimal carrier systems. To address this issue, the current study introduces solid lipid nanoparticles encapsulating hydrophobic dexamethasone palmitate (DXMS-Pal-SLNs) as an anti-inflammatory nanoplatform to treat pneumonia. The specialized nanoparticle formulation is characterized by high drug loading efficiency, low drug leakage and excellent colloidal stability in particular during nebulization and is proficiently designed to target alveolar macrophages in deep lung regions via local delivery with the nebulization administration. In vitro analyses revealed substantial reductions in the secretions of tumor necrosis factor-α and interleukin-6 from alveolar macrophages, highlighting the potential efficacy of DXMS-Pal-SLNs in alleviating pneumonia-related inflammation. Similarly, in vivo experiments showed a significant reduction in the levels of these cytokines in the lungs of mice experiencing lipopolysaccharide-induced pulmonary inflammation after the administration of DXMS-Pal-SLNs via nebulization. Furthermore, the study demonstrated that DXMS-Pal-SLNs effectively control acute infections without causing pulmonary infiltration or excessive recruitment of immunocytes in lung tissues. These findings highlight the potential of nebulized DXMS-Pal-SLNs as a promising therapeutic strategy for mitigating pneumonia-related inflammations. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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9. Histological Assessment of Respiratory Tract and Liver of BALB/c Mice Nebulized with Tocilizumab.
- Author
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de Andres, Paloma Jimena, Ferreiro, Sergio, Flores, Angela, Garcia, Almudena, and Henriquez-Camacho, Cesar
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NASAL mucosa , *ALVEOLAR macrophages , *DRUG absorption , *SALINE solutions , *BLOOD flow , *LUNGS - Abstract
Pulmonary drug delivery offers a minimally invasive and efficient method for treating lung conditions, leveraging the lungs' extensive surface area and blood flow for rapid drug absorption. Nebulized therapies aim to deliver drugs directly to the lung tissue. This study investigates the histological impact of nebulized tocilizumab—a monoclonal antibody targeting IL-6, traditionally administered intravenously for rheumatoid arthritis and severe COVID-19—on a murine model. Thirty BALB/c mice were nebulized with tocilizumab (10 mg, 5 mg, and 2.5 mg) and six controls were nebulized with saline solution. They were euthanized 48 h later, and their organs (lungs, nasal mucosa, and liver) were analyzed by a microscopic histological evaluation. The results indicate that all the mice survived the 48 h post-nebulization period without systemic compromise. The macroscopic examination showed no abnormalities, and the histopathological analysis revealed greater lung vascular changes in the control group than in the nebulized animals, which is attributable to the euthanasia with carbon dioxide. Additionally, increased alveolar macrophages were observed in the nebulized groups compared to controls. No significant histological changes were observed in the liver, indicating the safety of nebulized tocilizumab. In conclusion, these findings suggest the potential of nebulized tocilizumab for treating pulmonary inflammation, warranting further research to establish its efficacy and safety in clinical settings. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
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10. Nebulized platelet-derived extracellular vesicles attenuate chronic cigarette smoke-induced murine emphysema.
- Author
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Xuan, Weixia, Wang, Shaohua, Alarcon-Calderon, Amarilys, Bagwell, Monique Simone, Para, Rachel, Wang, Faping, Zhang, Chujie, Tian, Xue, Stalboerger, Paul, Peterson, Timothy, Sabbah, Michael S., Du, Zeji, Sarrafian, Tiffany, Mahlberg, Ryan, Hillestad, Matthew L., Rizzo, Skylar A., Paradise, Christopher R., Behfar, Atta, and Vassallo, Robert
- Abstract
Chronic obstructive pulmonary disease (COPD) is a prevalent lung disease usually resulting from cigarette smoking (CS). Cigarette smoking induces oxidative stress, which causes inflammation and alveolar epithelial cell apoptosis and represents a compelling therapeutic target for COPD. Purified human platelet-derived exosome product (PEP) is endowed with antioxidant enzymes and immunomodulatory molecules that mediate tissue repair. In this study, a murine model of CS-induced emphysema was used to determine whether nebulized PEP can influence the development of CS-induced emphysema through the mitigation of oxidative stress and inflammation in the lung. Nebulization of PEP effectively delivered the PEP vesicles into the alveolar region, with evidence of their uptake by type I and type II alveolar epithelial cells and macrophages. Lung function testing and morphometric assessment showed a significant attenuation of CS-induced emphysema in mice treated with nebulized PEP thrice weekly for 4 weeks. Whole lung immuno-oncology RNA sequencing analysis revealed that PEP suppressed several CS-induced cell injuries and inflammatory pathways. Validation of inflammatory cytokines and apoptotic protein expression on the lung tissue revealed that mice treated with PEP had significantly lower levels of S100A8/A9 expressing macrophages, higher levels of CD4+/FOXP3+ Treg cells, and reduced NF-κB activation, inflammatory cytokine production, and apoptotic proteins expression. Further validation using in vitro cell culture showed that pretreatment of alveolar epithelial cells with PEP significantly attenuated CS extract-induced apoptotic cell death. These data show that nebulization of exosomes like PEP can effectively deliver exosome cargo into the lung, mitigate CS-induced emphysema in mice, and suppress oxidative lung injury, inflammation, and apoptotic alveolar epithelial cell death. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
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11. Pilot study to evaluate the Effectiveness of Novel Designed Nebulizer (NDN) on Compliance among Preschoolers undergoing nebulization in selected Hospital, Salem.
- Author
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Immaculate, Stephina and Tamizharasi, K.
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PATIENT compliance ,RESPIRATORY therapy ,CLINICAL trials ,BLIND experiment ,RESPIRATORY diseases ,HOSPITAL patients ,JUDGMENT sampling ,DESCRIPTIVE statistics ,MANN Whitney U Test ,THEOPHYLLINE ,TREATMENT effectiveness ,CONTROL groups ,PRE-tests & post-tests ,NEBULIZERS & vaporizers ,DRUG efficacy ,RESEARCH methodology ,FRIEDMAN test (Statistics) ,DRUGS ,EVALUATION ,CHILDREN - Abstract
The present pilot study is as a part of Ph.D. Programme under THE TAMILNADU Dr. M.G.R Medical University. The study was designed to assess the effectiveness of Novel Designed Nebulizer (NDN) Compliance among Pre- schoolers undergoing nebulization in selected Hospital, Salem. Quasi experimental research design was adopt- ed. 20 children (10 experimental, 10 control group) who were admitted in pediatric wards with respiratory con- ditions and receiving nebulization between the age group of 3 to 6 years were selected by purposive sampling. Nebulization was given to experimental group with Novel Designed Nebulizer (NDN) and control group with routine nebulization. The results showed that the post test scores reveal that experimental group all 10(10%) the preschoolers were having high level of compliance and in control group all 10(10%) the preschoolers were having Medium level of compliance to nebulization procedure.The Friedman test value is 9.235 which is greater than the table value which indicates that the Novel designed nebulization is effective in increasing the compli- ance in experimental group.overall mean and SD in Experimental group is 2.31±0.23and the overall mean and SD in control group is 4.19 ±0.46. The mean difference is 1.88. The Z value is 3.800 which is higher than the table value which shows that the Novel designed nebulization is effective in improving the compliance among Preschoolers in experimental group. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
12. Comparison of nebulization with lignocaine and dexamethasone for attenuation of post-operative sore throat: A randomized controlled trial
- Author
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Rahul Saini, Sanjay Johar, Rahul Deora, Pritam Yadav, Preeti Gehlaut, and Prashant Kumar
- Subjects
post-operative sore throat ,lignocaine ,nebulization ,dexamethasone ,Medicine - Abstract
Background: Post-operative sore throat (POST) is a very common anesthesia-related event which may hamper patient satisfaction and increase treatment cost. Aims and Objectives: This study aimed to compare the effectiveness of nebulization with dexamethasone and lignocaine to prevent POST. Materials and Methods: This randomized controlled study involved 135 patients randomly divided into three equal groups: Group D – (n=45) received 8 mg (2 mL) dexamethasone plus 3 mL of distilled water, Group L – (n=45) received 80 mg (4% lignocaine 2 mL) plus 3 mL distilled, and Group S – (n=45) received nebulization with 5 mL normal saline. Results: At 4 h postoperatively, the incidence of POST was 15.6% in the dexamethasone group, 33.3% in the lignocaine group, and 73.3% in the saline group. The score was significantly higher in the saline group (P=0.001) compared to the dexamethasone and lignocaine group, while it was comparable between dexamethasone and lignocaine group (P=0.188). Similarly, dexamethasone and lignocaine groups were comparable at immediately post-operative, 2, 8, 12, and 24 h post-operative time points. Post-operative hoarseness scores of all three groups were comparable at all-time points. A significant increase in the heart rate (HR) and mean arterial pressure (MAP) after intubation was observed in the saline and dexamethasone group as compared to the lignocaine group (P=0.001) while HR and MAP were comparable in dexamethasone and saline group (P=1.000). Conclusion: Dexamethasone and lignocaine nebulization are both effective and comparable prophylaxis for POST and lignocaine nebulization has added advantage of blunting pressor response to endotracheal intubation over dexamethasone nebulization.
- Published
- 2024
- Full Text
- View/download PDF
13. Nebulization Versus Spray-as-You-Go Airway Topical Anesthesia Using Dexmedetomidine and Lidocaine Mixture During Awake Flexible Fiberoptic Intubation in Temporomandibular Ankylosis
- Author
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Mahmoud Hassan El-Baradei, Resident of Anesthesiology, Surgical Intensive Care and Pain Medicine
- Published
- 2023
14. Core 2.0 Nebulization Technique - A Sustainable Denim Finishing Approach
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Shah Md. Maruf Hasan, Md. Nahid-Ull-Islam, Md. Kamrul Hassan Chowdhury, Mahmuda Akter, and Md Shahidul Islam Sakib
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denim ,sustainability ,nebulization ,core machine ,environmental impact measuring (eim) software ,Textile bleaching, dyeing, printing, etc. ,TP890-933 ,Large industry. Factory system. Big business ,HD2350.8-2356 - Abstract
Sustainability has emerged as a key concept in the modern techniques of washing and treating denim. The clothing industry, specifically the denim washing sector, confronts substantial sustainability issues due to its impact on water pollution and the generation of large quantities of chemical waste. This research aims to investigate the long-term sustainability of the nebulization process in the context of denim finishing. The goal is to assess the dependability of the technology as a feasible option by demonstrating its effectiveness using environmental impact measuring software. The environmental impact assessment assesses the ecological consequences of clothing across four fundamental areas: water usage, chemical utilisation, energy consumption in the production process, and labour implications. The aforementioned methods proved to have low energy and water demands, resulting in less waste and pollution. This study evaluated several aspects of nebulization on denim and concluded that nebulization is a beneficial method for applying eco-friendly finishing.
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- 2024
- Full Text
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15. Efficient Generation of Plasma‐Activated Aerosols with High Concentrations of Reactive Species via Silicon Dioxide Coated Surface Acoustic Wave Devices.
- Author
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Chew, Nicholas S. L., Roudini, Mehrzad, Winkler, Andreas, Ooi, Chien W., Yeo, Leslie Y., and Tan, Ming K.
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ACOUSTIC surface wave devices , *MICROBIOLOGICAL aerosols , *SILICA , *ACOUSTIC surface waves , *AEROSOLS , *SURFACE coatings - Abstract
An effective method to deliver plasma‐activated water onto contaminated surfaces is with a surface acoustic wave (SAW) nebulizer that can efficiently produce plasma‐activated aerosols. While higher reactive species concentrations in the plasma‐activated aerosols promote more efficient bacterial inactivation and hence reduce the amount of aerosols needed to completely eradicate the bacteria colonies, higher concentrations of reactive species themselves are found to lead to lower nebulization rates due to the increased electrical conductivity of the solution. To circumvent this problem, it is shown that coating the SAW substrate with a thin insulating layer of silicon dioxide (SiO2) can significantly mitigate the nebulization rate reduction. Specifically, when the electrical conductivity increases from 0.25 to 4.0 mS cm−1, the 62% drop in the nebulization rate is observed with the uncoated SAW devices is substantially reduced to 19% simply with the SiO2 coating. This can be attributed to the synergistic effects of increased wettability and the suppression of the electrical body force acting on the liquid film. The concomitant increase in the bacterial colony reduction from 17% to 76% demonstrates this solution to be a facile yet effective way of enhancing the efficiency for spray‐based bacterial inactivation on contaminated surfaces. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
16. The Effects of Inspiratory Flows, Inspiratory Pause, and Suction Catheter on Aerosol Drug Delivery with Vibrating Mesh Nebulizers During Mechanical Ventilation.
- Author
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Lin, Hui-Ling, Fink, James B., and Li, Jie
- Subjects
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ARTIFICIAL respiration , *NEBULIZERS & vaporizers , *AEROSOLS , *CATHETERS , *ULTRAVIOLET spectrophotometry - Abstract
Background: Some experts recommend specific ventilator settings during nebulization for mechanically ventilated patients, such as inspiratory pause, high inspiratory to expiratory ratio, and so on. However, it is unclear whether those settings improve aerosol delivery. Thus, we aimed to evaluate the impact of ventilator settings on aerosol delivery during mechanical ventilation (MV). Methods: Salbutamol (5.0 mg/2.5 mL) was nebulized by a vibrating mesh nebulizer (VMN) in an adult MV model. VMN was placed at the inlet of humidifier and 15 cm away from the Y-piece of the inspiratory limb. Eight scenarios with different ventilator settings were compared with endotracheal tube (ETT) connecting 15 cm from the Y-piece, including tidal volumes of 6–8 mL/kg, respiratory rates of 12–20 breaths/min, inspiratory time of 1.0–2.5 seconds, inspiratory pause of 0–0.3 seconds, and bias flow of 3.5 L/min. In-line suction catheter was utilized in two scenarios. Delivered drug distal to the ETT was collected by a filter, and drug was assayed by an ultraviolet spectrophotometry (276 nm). Results: Compared to the use of inspiratory pause, the inhaled dose without inspiratory pause was either higher or similar across all ventilation settings. Inhaled dose was negatively correlated with inspiratory flow with VMN placed at 15 cm away from the Y-piece (rs = −0.68, p < 0.001) and at the inlet of humidifier (rs = −0.83, p < 0.001). The utilization of in-line suction catheter reduced inhaled dose, regardless of the ventilator settings and nebulizer placements. Conclusions: When VMN was placed at the inlet of humidifier, directly connecting the Y-piece to ETT without a suction catheter improved aerosol delivery. In this configuration, the inhaled dose increased as the inspiratory flow decreased, inspiratory pause had either no or a negative impact on aerosol delivery. The inhaled dose was greater with VMN placed at the inlet of humidifier than 15 cm away the Y-piece. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
17. Nebulized Dexmedetomidine Alleviates Oxidative Stress in Ventilator-induced Lung Injury via Keap1-Nrf2-ARE Pathway.
- Author
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Jun Zha, Youjia Yu, Ji Zhu, Guiru Li, Xiaolin Deng, and Hong Xie
- Subjects
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OXIDATIVE stress , *POSITIVE end-expiratory pressure , *LUNG injuries , *DEXMEDETOMIDINE , *HEMATOXYLIN & eosin staining - Abstract
This study aimed to explore the underlying mechanism of nebulized dexmedetomidine (DEX) in ameliorating ventilator-induced lung injury (VILI)-induced oxidative stress in rats. Forty 7 to 8-week-old Sprague-Dawley rats at the specific pathogen-free level were randomized into the control group, model group, nebulized dexmedetomidine (WH-YM) group, and dexmedetomidine intravenous infusion (JM-YM) group, each containing 10 rats. Except for the control group, rats in the other groups underwent mechanical ventilation (tidal volume, 40 mL/kg; respiratory rate, 70 breaths per minute; inspiratory-to-expiratory ratio, 1:2; fraction of inspired oxygen, 21%; positive end-expiratory pressure, 0 cmH2O). Nebulized DEX (6.3 µg/kg), and isodose intravenous DEX were given to rats of WH-YM and JM-YM groups prior to ventilation. Post 4-hour ventilation, rats were euthanized. Lung tissue wet-to-dry weight ratio, H&E staining for assessing diffuse alveolar damage (DAD), and expression levels of Nrf2 and Keap1 detected by qRT-PCR and Western blot were compared. Inflammatory markers TNF-α, IL-2, and IL-6, and oxidative stress indices malondialdehyde (MDA) and superoxide dismutase (SOD), were quantified in lung tissues and serum samples using commercial kits. Rats in the WH-YM and JM-YM groups demonstrated significant ameliorations in the wetto- dry weight ratio and DAD score, decreased Keap1, TNF-α, IL-2, and IL-6 levels in lung tissues and serum samples, but increased Nrf2 and SOD level than those of controls. These changes were more pronounced in the WH-YM group than in the JM-YM group. DEX effectively alleviates VILI-induced oxidative stress and inflammation via the Keap1- Nrf2-ARE signaling pathway., especially in the nebulized administration. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
18. Aerosolized Antifungals for the Treatment of Pulmonary Fungal Diseases.
- Author
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Muthu, Valliappan, Sehgal, Inderpaul Singh, and Agarwal, Ritesh
- Abstract
Purpose of Review: Inhaled antifungals achieve high lung concentrations with minimal systemic adverse events. Despite being available for over five decades, data supporting their use outside the mold-active prophylaxis setting are lacking. Herein, we review the recent evidence on inhaled antifungals in treating pulmonary aspergillosis (allergic, chronic, and invasive) and mucormycosis. Recent Findings: Several antifungals have recently been evaluated, including repurposed and newer inhaled agents (opelconazole). Inhaled amphotericin B deoxycholate (in pulmonary mucormycosis) and liposomal formulation (in invasive aspergillosis) were safely used in two recent randomized trials. However, both these small trials failed to demonstrate added benefit with adjunctive inhaled amphotericin. In allergic bronchopulmonary aspergillosis, nebulized amphotericin B may prolong the time-to-exacerbation when used in patients experiencing frequent exacerbations. Summary: The last decade has witnessed considerable progress in inhaled antifungals. Ongoing trials will likely offer hope for managing allergic and invasive pulmonary mycoses in the future. [ABSTRACT FROM AUTHOR]
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- 2024
- Full Text
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19. Three-minute nebulization of gentamicin in healthy dogs results in therapeutic concentrations in bronchoalveolar lavage fluid while remaining below the toxic range values in blood.
- Author
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Lyssens, Aurélie, Biénès, Tom, Fastrès, Aline, Machiels, Hélène, Moreau, Camille, Tutunaru, Alexandru-Cosmin, Deville, Marine, Charlier, Corinne, Clercx, Cécile, and Billen, Frédéric
- Subjects
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GENTAMICIN , *BRONCHOALVEOLAR lavage , *DOGS , *GENERAL anesthesia , *FLUIDS - Abstract
OBJECTIVE: To determine and compare the concentration of gentamicin in the lower airways and serum of healthy spontaneously breathing dogs after nebulization with 5% undiluted gentamicin during 3 versus 10 minutes. ANIMALS: 10 healthy experimental Beagles. METHODS: This was a prospective crossover study. A standardized bronchoalveolar lavage (BAL) procedure was performed in each dog after 1 week of administration of each of 2 different gentamicin nebulization protocols separated by a 1-week washout period. The 2 protocols consisted of nebulization of 5% undiluted gentamicin (50 mg/mL) twice daily either during 10 minutes per session (± 95 mg; 10-minute protocol) or 3 minutes per session (± 28 mg; 3-minute protocol). BAL fluid (BALF) was obtained under general anesthesia using a bronchoscope within 15 minutes after administration of the last nebulization. Blood was collected within 5 minutes after BALF collection. BALF and serum gentamicin concentrations were determined by particle-enhanced turbidimetric inhibition immunoassay. Concentrations between protocols were compared using a paired t test. RESULTS: Both BALF and serum gentamicin concentrations were higher after the 10-minute protocol compared with the 3-minute protocol (mean ± SD: 2.41 ± 0.87 mg/L vs 1.25 ± 0.31 mg/L, P = .001; and 1.02 ± 0.59 mg/L vs 0.31 ± 0.24 mg/L, P < .0001 in BALF and serum, respectively), while the BALF-to-serum ratio did not differ between the protocols (3.75 [1.37 to 5.75] (median [IQR]) in the 3-minute protocol vs 2.48 [2.02 to 2.67] in the 10-minute protocol; P = .754). CLINICAL RELEVANCE: A 3-minute nebulization of gentamicin seems to achieve sufficient concentrations of gentamicin in the BALF to have good efficacy against aminoglycoside-sensitive bacteria while remaining below the toxic range values in blood. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
20. Impact of Ultrasonic Vibrations on the Stability of Bovhyaluronidase Azoximer in Solution.
- Author
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Krasheninnikov, A. E., Sepp, V. V., Bakulin, K. S., Arefieva, O. I., and Karsanov, A. A.
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ULTRASONICS , *NEBULIZERS & vaporizers , *HYALURONIDASES , *ULTRASONIC imaging , *RADIATION , *ULTRASONIC welding - Abstract
Changes in the quantitative content and specific activity of hyaluronidase enzyme after ultrasonic treatment of solutions of bovhyaluronidase azoximer were studied. Solutions of the finished dosage form of bovhyaluronidase azoximer (Longidase® lyophilizate for preparation of solution for injection, 3000 IE) and bovhyaluronidase azoximer drug substance (Longidase® substance-lyophilizate) were studied. The aim of the study was to determine the effect of ultrasound (US) on the stability of bovhyaluronidase azoximer determined from the measured quantitative content and enzymatic activity of the substance in solution. US at a radiation intensity of 61.4 W/L during treatment in an ultrasonic bath did not significantly affect the quantitative content of the bovhyaluronidase azoximer drug substance in solution, while the decrease in the enzymatic activity of the active substance in Longidase solution after 20 and 30 min of ultrasonic treatment was statistically significant. At the same time, the enzymatic activity of the active substance determined by a biochemical method differed significantly (residual activity from 99.70% to 59.27%) depending on the design features of the nebulizers and the nebulization time after nebulization of the bovhyaluronidase azoximer solution in mesh nebulizers from various manufacturers. Nebulization times of min were least associated with the risk of significant loss of stability of bovhyaluronidase azoximer. [ABSTRACT FROM AUTHOR]
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- 2024
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21. Treatment of COVID-19 by Nebulization of Inteferon Beta 1b Efficiency and Safety Study (COV-NI)
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- 2023
22. Novel inhalation therapy in pulmonary fibrosis: principles, applications and prospects
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Meiling Zheng, Wei Zhu, Fei Gao, Yu Zhuo, Mo Zheng, Guanghao Wu, and Cuiling Feng
- Subjects
Inhalation therapy ,Pulmonary fibrosis ,Nebulization ,Dry powder ,Nanomedicine ,Biotechnology ,TP248.13-248.65 ,Medical technology ,R855-855.5 - Abstract
Abstract Pulmonary fibrosis (PF) threatens millions of people worldwide with its irreversible progression. Although the underlying pathogenesis of PF is not fully understood, there is evidence to suggest that the disease can be blocked at various stages. Inhalation therapy has been applied for lung diseases such as asthma and chronic obstructive pulmonary disease, and its application for treating PF is currently under consideration. New techniques in inhalation therapy, such as the application of microparticles and nanoparticles, traditional Chinese medicine monomers, gene therapy, inhibitors, or agonists of signaling pathways, extracellular vesicle interventions, and other specific drugs, are effective in treating PF. However, the safety and effectiveness of these therapeutic techniques are influenced by the properties of inhaled particles, biological and pathological barriers, and the type of inhalation device used. This review provides a comprehensive overview of the pharmacological, pharmaceutical, technical, preclinical, and clinical experimental aspects of novel inhalation therapy for treating PF and focus on therapeutic methods that significantly improve existing technologies or expand the range of drugs that can be administered via inhalation. Although inhalation therapy for PF has some limitations, the advantages are significant, and further research and innovation about new inhalation techniques and drugs are encouraged. Graphical Abstract
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- 2024
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23. The effect of various inhaled asthma medications on the color stability of paediatric dental restorative materials
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Merve Candan and Murat Ünal
- Subjects
Asthma medication ,Color stability ,Dental restorative materials ,Nebulization ,Pediatric dentistry ,Dentistry ,RK1-715 - Abstract
Abstract Background The purpose of the study is to analyse the effects of different inhaled asthma medications (IAMs) on the color change of dental restorative materials (DRMs). Methods In total, 192 samples were taken from six different DRMs: [Filtek Z550 (nanohybrid composite), Fusio Liquid Dentin (Self-adhering flowable composite), Filtek Ultimate (nanofilled flowable composite), Dyract XP (compomer), Fuji II LC (resin-modified glass ionomer), Fuji IX Fast (self-cured-packable glass ionomer), (n = 32)]. After the initial color values (CIELab) of DRMs were measured by using a spectrophotometer, each sample was exposed to the same IAMs via nebulizer according to the four different inhaled therapies and measurements were repeated on the 7th & 21st days. Results In all IAM groups, DRM with the least amount of ΔE was nanohybrid composite, while the highest ΔE was found in Fuji II LC. Among all experimental groups, only Fuji II LC which was administered the combined medication, exceeded the clinically unacceptable threshold (ΔE = 3.3) on 7th & 21st days. Conclusions Consequently, important factors affecting the susceptibility to color stability are the type of IAMs, the administration time-dosage, and the type of DRMs.
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- 2024
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24. Novel inhalation therapy in pulmonary fibrosis: principles, applications and prospects.
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Zheng, Meiling, Zhu, Wei, Gao, Fei, Zhuo, Yu, Zheng, Mo, Wu, Guanghao, and Feng, Cuiling
- Subjects
- *
RESPIRATORY therapy , *PULMONARY fibrosis , *INHALERS , *CHRONIC obstructive pulmonary disease , *CHINESE medicine , *EXTRACELLULAR vesicles - Abstract
Pulmonary fibrosis (PF) threatens millions of people worldwide with its irreversible progression. Although the underlying pathogenesis of PF is not fully understood, there is evidence to suggest that the disease can be blocked at various stages. Inhalation therapy has been applied for lung diseases such as asthma and chronic obstructive pulmonary disease, and its application for treating PF is currently under consideration. New techniques in inhalation therapy, such as the application of microparticles and nanoparticles, traditional Chinese medicine monomers, gene therapy, inhibitors, or agonists of signaling pathways, extracellular vesicle interventions, and other specific drugs, are effective in treating PF. However, the safety and effectiveness of these therapeutic techniques are influenced by the properties of inhaled particles, biological and pathological barriers, and the type of inhalation device used. This review provides a comprehensive overview of the pharmacological, pharmaceutical, technical, preclinical, and clinical experimental aspects of novel inhalation therapy for treating PF and focus on therapeutic methods that significantly improve existing technologies or expand the range of drugs that can be administered via inhalation. Although inhalation therapy for PF has some limitations, the advantages are significant, and further research and innovation about new inhalation techniques and drugs are encouraged. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
25. Inhaled "Muco‐Trapping" Monoclonal Antibody Effectively Treats Established Respiratory Syncytial Virus (RSV) Infections.
- Author
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McSweeney, Morgan D., Alnajjar, Sarhad, Schaefer, Alison M., Richardson, Zach, Wolf, Whitney, Stewart, Ian, Sriboonyapirat, Pun, McCallen, Justin, Farmer, Ellen, Nzati, Bernadette, Lord, Sam, Farrer, Brian, Moench, Thomas R., Kumar, Priya A., Arora, Harendra, Pickles, Raymond J., Hickey, Anthony J., Ackermann, Mark, and Lai, Samuel K.
- Subjects
- *
RESPIRATORY syncytial virus , *LUNGS , *MONOCLONAL antibodies , *VIRAL load , *BRONCHOALVEOLAR lavage , *PALIVIZUMAB , *RESPIRATORY infections , *PHEROMONE traps - Abstract
Respiratory syncytial virus (RSV) causes substantial morbidity and mortality in infants, the immunocompromised, and the elderly. RSV infects the airway epithelium via the apical membrane and almost exclusively sheds progeny virions back into the airway mucus (AM), making RSV difficult to target by systemically administered therapies. An inhalable "muco‐trapping" variant of motavizumab (Mota‐MT), a potent neutralizing mAb against RSV F is engineered. Mota‐MT traps RSV in AM via polyvalent Fc‐mucin bonds, reducing the fraction of fast‐moving RSV particles in both fresh pediatric and adult AM by ≈20–30‐fold in a Fc‐glycan dependent manner, and facilitates clearance from the airways of mice within minutes. Intranasal dosing of Mota‐MT eliminated viral load in cotton rats within 2 days. Daily nebulized delivery of Mota‐MT to RSV‐infected neonatal lambs, beginning 3 days after infection when viral load is at its maximum, led to a 10 000‐fold and 100 000‐fold reduction in viral load in bronchoalveolar lavage and lung tissues relative to placebo control, respectively. Mota‐MT‐treated lambs exhibited reduced bronchiolitis, neutrophil infiltration, and airway remodeling than lambs receiving placebo or intramuscular palivizumab. The findings underscore inhaled delivery of muco‐trapping mAbs as a promising strategy for the treatment of RSV and other acute respiratory infections. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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26. Intrapulmonary and Systemic Pharmacokinetics of Colistin Following Nebulization of Low-Dose Colistimethate Sodium in Patients with Ventilator-Associated Pneumonia Caused by Carbapenem-Resistant Acinetobacter baumannii.
- Author
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Lee, Dong-Hwan, Kim, Shin-Young, Kim, Yong-Kyun, Jung, So-Young, Jang, Ji-Hoon, Jang, Hang-Jea, and Lee, Jae-Ha
- Subjects
CARBAPENEM-resistant bacteria ,VENTILATOR-associated pneumonia ,ACINETOBACTER baumannii ,COLISTIN ,PHARMACOKINETICS - Abstract
Colistimethate sodium (CMS) nebulization is associated with reduced systemic toxicity compared to intravenous injection, with potentially enhanced clinical efficacy. This study aimed to assess the pharmacokinetic (PK) properties of colistin during low-dose CMS nebulization in patients with ventilator-associated pneumonia (VAP) caused by carbapenem-resistant Acinetobacter baumannii. A nonlinear mixed-effects modeling approach was applied to develop population PK models for colistin in both epithelial lining fluid (ELF) and plasma. Twenty patients participated, and 80 ELF and 100 plasma samples were used for model development. Median colistin concentrations measured in ELF were 614-fold, 408-fold, and 250-fold higher than in plasma at 1, 3, and 5 h, respectively. Time courses in both ELF and plasma were best described by a one-compartment model with a Weibull absorption process. When the final model was simulated, the maximum free concentration and area under the free colistin concentration–time curve at steady state over 24 h in the plasma were approximately 1/90 and 1/50 of the corresponding values in ELF at steady state, respectively. For an A. baumannii MIC of 1 mg/L, inhaling 75 mg of CMS at 6 h intervals was deemed appropriate, with dose adjustments needed for MICs exceeding 2 mg/L. Using a nebulizer for CMS resulted in a notably higher exposure of colistin in the ELF than plasma, indicating the potential of nebulization to reduce systemic toxicity while effectively treating VAP. [ABSTRACT FROM AUTHOR]
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- 2024
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27. Measuring of cardiac electrical system function among salbutamol inhaled children -- A cohort study.
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Anebaracy V., S. S., Kumaran, and Rekha C.
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ARRHYTHMIA ,HEART ,ATRIOVENTRICULAR node ,ALBUTEROL ,BETA adrenoceptors ,ADRENERGIC beta agonists ,ADRENERGIC agonists - Abstract
This article examines the effects of inhaled salbutamol on the cardiac electrical system in children. The study found that salbutamol inhalation led to changes in heart rate and certain electrocardiogram parameters, indicating a potential increased risk of arrhythmias. The study emphasizes the need for cautious use of high doses of salbutamol in children with asthma. The researchers also found that salbutamol can affect the T wave axis in the heart, increasing the risk of cardiac re-entry and arrhythmias. It is important to note that these findings may not apply to all populations and further research is needed. [Extracted from the article]
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- 2024
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28. Role of preoperative dexamethasone nebulization versus ketamine nebulization in reducing complications of nasogastric tube after laparoscopic surgery.
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Zakaria, Mai Mohammed, Aboulella, Sahar Kamal Mohammed, Hassanein, Sahar Mohammed Kamal, El Hennawy, Ahmed Mohammed Elsayed, and El Din Shabana, Tarek Samir Salah
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- *
NASOENTERAL tubes , *LAPAROSCOPIC surgery , *KETAMINE , *DEXAMETHASONE , *ABDOMINAL surgery , *SURGERY - Abstract
Background & objective: Nasogastric (NG) tube insertion is a frequently performed medical procedure. Most of the time, the oropharyngeal discomfort is usually underestimated, and insertions are carried out with just regular lubricant jelly and no additional painkillers, which results in less-than-ideal pain management. We compared the effectiveness of preemptive use of nebulized ketamine and dexamethasone in preventing post-operative sore throat following NG tube insertion in laparoscopic surgery. Methods: This prospective randomized double-blind controlled study was carried on 108 patients of both sexes with ASA physical status I-II. aged 20-60 years, undergoing laparoscopic abdominal surgeries under general anesthesia. Patients were randomized into 3 equal groups. Group D received a dose of 8 mg nebulized dexamethasone. Group K received 50 mg nebulized ketamine. Group S received 5 ml of nebulized saline. NG tube was passed after induction of general anesthesia. Postoperative sore throat was enquired from the patients at full recovery (zero hour), then at 2, 8, 12 and 24 h. Computer software (SPSS 15.0.1. for Windows; SPSS Inc, Chicago, IL, 2001) was used for data analysis. Non-parametric quantitative data, median, range, percentages, and numbers are given for qualitative data. The appropriate analysis was conducted in line with the data obtained. P < 0.05 was regarded as significant. Results: The incidence of postoperative sore throat (POST) was lower with both nebulized dexamethasone and nebulized ketamine compared with nebulized saline; and significantly lower with dexamethasone compared with ketamine at 0 h (P < 0.001), 2 h (P < 0.001), 8 h (P < 0.001), 12 h (P < 0.001), and 24 h (P < 0.001). Conclusion: The current study revealed that preoperative nebulized dexamethasone was more effective than nebulized ketamine in reducing postoperative sore throat. [ABSTRACT FROM AUTHOR]
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- 2024
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29. Towards More Precise Targeting of Inhaled Aerosols to Different Areas of the Respiratory System.
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Sosnowski, Tomasz R.
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- *
RESPIRATORY organs , *AEROSOLS , *SMART devices , *MASS transfer , *PARTICLE interactions - Abstract
Pharmaceutical aerosols play a key role in the treatment of lung disorders, but also systemic diseases, due to their ability to target specific areas of the respiratory system (RS). This article focuses on identifying and clarifying the influence of various factors involved in the generation of aerosol micro- and nanoparticles on their regional distribution and deposition in the RS. Attention is given to the importance of process parameters during the aerosolization of liquids or powders and the role of aerosol flow dynamics in the RS. The interaction of deposited particles with the fluid environment of the lung is also pointed out as an important step in the mass transfer of the drug to the RS surface. The analysis presented highlights the technical aspects of preparing the precursors to ensure that the properties of the aerosol are suitable for a given therapeutic target. Through an analysis of existing technical limitations, selected strategies aimed at enhancing the effectiveness of targeted aerosol delivery to the RS have been identified and presented. These strategies also include the use of smart inhaling devices and systems with built-in AI algorithms. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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30. Dataset on exposure conditions to Fe2O3 and SiO2 colloidal suspension and airborne particulate matter (PM) suspensions: crude NIST1648a and with reduced content of organic matter, LAp120
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Monika Jankowska-Kieltyka, Adam Roman, and Irena Nalepa
- Subjects
Particulate matter ,Nanoparticles ,Nebulization ,Inhalation ,Suspensions ,Absorbance ,Computer applications to medicine. Medical informatics ,R858-859.7 ,Science (General) ,Q1-390 - Abstract
Particulate matter (PM) present in the air pollution increases morbidity and mortality due to several reasons. The dataset presents a comparative analysis of nebulization process of Fe2O3 and SiO2 nanoparticles or crude PM (NIST1648a) and that with reduced content of organic matter (LAp120). Nebulization tests were carried out to determine concentrations of nanoparticle and PM suspensions, in order to create an atmosphere with a concentration of PM particles about 1000 µg/m3 of air in the exposure chambers. It is important to properly recreate environmental conditions during further research on animals. The absorbance spectrum of the suspensions of the tested materials was measured in the range of 300–700 nm. The changes in the absorbance of these suspensions depending on the concentration after their passage through the nebulizers were examined. Based on the absorbance, it was determined to what extent the suspensions are passed out and dispersed by the nebulizers. The operating mode of the nebulizers and the concentration of suspensions were determined in order to establish the optimal exposure conditions and the microclimate of the chambers for further studies with mice. The dataset can help in optimization of nebulization process for all researchers exploring the further issue of the influence of the air pollution on the broadly understood animal functions, behavioral parameters and biochemical aspects.
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- 2024
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31. Comparison of the effects of ketamine via nebulization versus different pharmacological approaches in pediatric sedation: a systematic review and meta-analysis of randomized controlled trials
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Xiao Liu, Bingchen Lang, Linan Zeng, Liang Huang, Shouming Chen, Zhi-Jun Jia, Guo Cheng, Qin Yu, and Lingli Zhang
- Subjects
Nebulization ,Ketamine ,Sedation ,Pediatrics ,Meta-analysis ,Anesthesiology ,RD78.3-87.3 - Abstract
Abstract Background Nebulized drug delivery is commonly used in pediatric clinical practice. The growing number of literatures have reported the application of nebulized ketamine in pediatric sedation in recent years. This meta-analysis of randomized controlled trials comparing the efficacy and safety of nebulized ketamine versus different pharmacological approaches was conducted to estimate the effects of this technique in pediatric sedation. Methods We searched PubMed, Embase, and Cochrane Library from inception to Feb 2023. All randomized controlled trials used nebulized ketamine as presurgical and pre-procedural sedatives in children were included. Sedative effects and various adverse events were considered as the outcomes. Results Ten studies with 727 pediatric patients were enrolled. Compared to nebulized dexmedetomidine, using of ketamine via nebulization showed similar sedation satisfaction (54.79% vs. 60.69%, RR = 0.88, with 95%CI [0.61, 1.27]), success rate of parental separation (57.27% vs. 73.64%, RR = 0.81, with 95%CI [0.61, 1.08]), and mask acceptability (37.27% vs. 52.73%, RR = 0.71, with 95%CI [0.45, 1.10]). However, the using of combination of two medications (nebulized ketamine plus nebulized dexmedetomidine) was associated with better sedative satisfaction (33.82% vs. 68.11%, RR = 0.50, with 95%CI [0.27, 0.92]) and more satisfactory mask acceptance (45.59% vs. 71.01%, RR = 0.69, with 95%CI [0.56, 0.86]). Compared with nebulized ketamine, using of nebulized dexmedetomidine was associated with less incidence of emergence agitation (18.18% vs. 3.33%, RR = 4.98, with 95%CI [1.88, 13.16]). Conclusions Based on current evidences, compared to nebulized dexmedetomidine, nebulized ketamine provides inconspicuous advantages in pediatric sedation, and it has a relatively high incidence of emergence agitation. Combination of nebulized ketamine and dexmedetomidine might be considered as one preferred option in pediatric sedation as it can provide more satisfactory sedative effects. However, there is insufficient evidence regarding nebulized ketamine versus ketamine administered through other routes and nebulized ketamine versus other sedatives. The overall low or moderate quality of evidence evaluated by the GRADE system also calls for more high-quality studies with larger sample sizes in future. Research registration The protocol of present study was registered with PROSPERO (CRD42023403226).
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- 2023
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32. Nebulized Glutathione as a Key Antioxidant for the Treatment of Oxidative Stress in Neurodegenerative Conditions
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João Vitor Lana, Alexandre Rios, Renata Takeyama, Napoliane Santos, Luyddy Pires, Gabriel Silva Santos, Izair Jefthé Rodrigues, Madhan Jeyaraman, Joseph Purita, and Jose Fábio Lana
- Subjects
glutathione ,nebulization ,oxidative stress ,neurological disorders ,aging ,regenerative medicine ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Glutathione (GSH), a tripeptide synthesized intracellularly, serves as a pivotal antioxidant, neutralizing reactive oxygen species (ROS) and reactive nitrogen species (RNS) while maintaining redox homeostasis and detoxifying xenobiotics. Its potent antioxidant properties, particularly attributed to the sulfhydryl group (-SH) in cysteine, are crucial for cellular health across various organelles. The glutathione-glutathione disulfide (GSH-GSSG) cycle is facilitated by enzymes like glutathione peroxidase (GPx) and glutathione reductase (GR), thus aiding in detoxification processes and mitigating oxidative damage and inflammation. Mitochondria, being primary sources of reactive oxygen species, benefit significantly from GSH, which regulates metal homeostasis and supports autophagy, apoptosis, and ferroptosis, playing a fundamental role in neuroprotection. The vulnerability of the brain to oxidative stress underscores the importance of GSH in neurological disorders and regenerative medicine. Nebulization of glutathione presents a novel and promising approach to delivering this antioxidant directly to the central nervous system (CNS), potentially enhancing its bioavailability and therapeutic efficacy. This method may offer significant advantages in mitigating neurodegeneration by enhancing nuclear factor erythroid 2-related factor 2 (NRF2) pathway signaling and mitochondrial function, thereby providing direct neuroprotection. By addressing oxidative stress and its detrimental effects on neuronal health, nebulized GSH could play a crucial role in managing and potentially ameliorating conditions such as Parkinson’s Disease (PD) and Alzheimer’s Disease (AD). Further clinical research is warranted to elucidate the therapeutic potential of nebulized GSH in preserving mitochondrial health, enhancing CNS function, and combating neurodegenerative conditions, aiming to improve outcomes for individuals affected by brain diseases characterized by oxidative stress and neuroinflammation.
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- 2024
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33. Effect of ketamine nebulization on post-operative sore throat after general anaesthesia.
- Author
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C., Shalini, N., Lingaraju, Rudrappa, Charulatha, and K., Tejanand
- Subjects
- *
MIDDLE ear surgery , *KETAMINE , *THROAT , *ANESTHESIA , *METHYL aspartate receptors - Abstract
General anaesthesia with endotracheal intubation results in postoperative sore throat. We studied the effectiveness of nebulization with ketamine which is a NMDA receptor antagonist, on the incidence and severity of sore throat in patients undergoing surgery under general anaesthesia. Institutional ethical committee approval obtained. 100 consenting patients scheduled for middle ear surgeries were randomly allocated to two groups of 50 each to receive 50mg of Ketamine or saline nebulization 15 minutes before the induction. Sore throat was less in 0, 2, 4, and 6hrs (p<0.02). Ketamine nebulization before intubation reduces incidence and severity of sore throat. [ABSTRACT FROM AUTHOR]
- Published
- 2023
34. Nebulization versus metered-dose inhaler and spacer in bronchodilator responsiveness testing: a retrospective study.
- Author
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Lu, Rongli, Li, Ying, Hu, Chengping, Pan, Pinhua, Zhao, Qiaohong, and He, Ruoxi
- Subjects
METERED-dose inhalers ,CHRONIC obstructive pulmonary disease ,FORCED expiratory volume ,PROPENSITY score matching - Abstract
Background: The recommended delivery mode for bronchodilators in bronchodilator responsiveness (BDR) testing remains controversial. Objective: To compare the efficacy of salbutamol administration using a nebulizer versus a metered-dose inhaler (MDI) with spacer in BDR testing. Design: A retrospective study. Methods: This study examined the data of patients with chronic obstructive pulmonary disease who completed BDR testing between 1 December 2021 and 30 June 2022, at Xiangya Hospital, Central South University. After administering 400 μg of salbutamol through an MDI with spacer or 2.5 mg using a nebulizer, the changes in forced expiratory volume in one second (FEV
1 ) and forced vital capacity (FVC) were analyzed in patients with moderate-to-very severe spirometric abnormalities [pre-bronchodilator FEV1 percentage predicted values (FEV1 %pred) ⩽59%]. Significant responsiveness was assessed as >12% and >200 mL improvement in FEV1 and/or FVC or >10% increase in FEV1 %pred or FVC percentage predicted values (FVC%pred) from pre- to post-bronchodilator administration. Results: Of the enrolled 894 patients, 83.2% were male (median age, 63 years). After propensity score matching, 240 pairs of patients were selected. The increment in FEV1 and increased FEV1 relative to the predicted value (ΔFEV1 %pred) were significantly higher in patients <65 years and those with severe spirometric abnormalities in the nebulization group than patients in the MDI group (all p < 0.05). Compared with MDI with spacer, patients who used nebulization had a 30 mL greater increase in ΔFEV1 (95% CI: 0.01–0.05, p = 0.004) and a 1.09% greater increase in ΔFEV1 %pred (95% CI: 0.303–1.896, p = 0.007) from baseline. According to the > 12% and >200 mL increase criterion, the significant BDR rate with nebulization was 1.67 times higher than that with an MDI with spacer (OR = 1.67, 95% CI: 1.13–2.47, p = 0.009). Conclusion: Salbutamol delivered using a nebulizer may be preferable to an MDI with spacer in certain circumstances. Nebulization has the potential to increase responsiveness to salbutamol in BDR testing. Plain language summary: Nebulization versus metered-dose inhaler and spacer in bronchodilator responsiveness testing Bronchodilator responsiveness testing is commonly undertaken as an important part of spirometry testing to determine the degree of volume and airflow improvement after bronchodilator administration. BDR testing results may affect patients' diagnosis and treatment. This study compared the effects of two delivery models (a metered dose inhaler (MDI) with spacer and nebulization) on responsiveness to bronchodilators and the results of bronchodilator responsiveness testing among patients with chronic obstructive pulmonary disease. We found that the increment in forced expiratory volume in one second were significantly higher in patients aged <65 years and in those with severe spirometric abnormalities in the nebulization group than in those in the MDI group. The study provides evidence that salbutamol delivered by a nebulizer is preferable to an MDI with spacer in patients <65 years and in those with severe spirometric abnormalities and could increase positive responsiveness to bronchodilators. The study will assist in clinical decision-making by selecting the appropriate dosing regimen for different patients. [ABSTRACT FROM AUTHOR]- Published
- 2023
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35. Nebulized dexmedetomidine for preventing postoperative sore throat after tracheal intubation: a randomized, double‐blind clinical trial.
- Author
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Pradian, Erwin, Kestriani S. S., Nurita Dian, and Ritonga, Diva Zuniar
- Subjects
- *
DEXMEDETOMIDINE , *TRACHEA intubation , *CLINICAL trials , *THROAT , *INTENSIVE care units , *ENDOTRACHEAL suctioning - Abstract
Background and Objective: Endotracheal intubation is part of general anesthesia, and probably the most commonly performed airway related procedure in operating rooms as well as intensive care units. It may be a causative factor in about 74% of patients who experience postoperative sore throat (POST) due to airway mucosal injury. This doubleblind randomized clinical trial assessed the effectiveness of nebulized dexmedetomidine in preventing POST by comparing with a control group. Methodology: In this randomized, double‐blind clinical trial, we enrolled 56 patients, who were to undergo general anesthesia with endotracheal intubation. Patients were randomly divided into two groups of 28 each. Group A received dexmedetomidine nebulization, and Group B had normal saline nebulization, both for 15 min before induction. POST was assessed at 1, 2, 4, 6, 12, and 24 h after extubation using a POST scale. Data were collected and statistically analyzed Results: The overall incidence of POST was 60.3%: Control group experienced POST by 28 (96.6%) patients, compared to 7 (24,1%) patients in dexmedetomidine group (P = 0.0001). Differences were significant at recording times postoperatively. The dexmedetomidine group had milder sore throats, less coughing, and lower heart rates/blood pressures compared to the control group. Conclusion: Patients who were nebulized with dexmedetomidine, experienced lower rates and intensity of postoperative sore throat compared to the control group. Administering dexmedetomidine via nebulization before intubation can be considered a safe and effective method for reducing postoperative sore throat, with less postintubation hemodynamic derangement. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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36. The Impact of Various High-Flow Nasal Cannula Devices on Transnasal Aerosol Delivery.
- Author
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Alanazi, Osama and Jie Li
- Subjects
DRUG delivery systems ,IN vitro studies ,BIOLOGICAL models ,EXPERIMENTAL design ,NASAL cannula ,AEROSOLS ,RESPIRATORY insufficiency ,ALBUTEROL ,HUMAN anatomical models ,SIMULATION methods in education ,NEBULIZERS & vaporizers ,TREATMENT effectiveness ,COMPARATIVE studies ,INTRANASAL administration ,DESCRIPTIVE statistics ,INHALATION administration ,DATA analysis software ,SPECTROPHOTOMETRY - Abstract
BACKGROUND: Aerosol delivery via high-flow nasal cannula (HFNC) has gained popularity due to the increased use of the modality for treating hypoxemic and hypercapnic respiratory failure. Various HFNC devices are available in the United States; however, the effectiveness of aerosol delivery via HFNC devices remains unclear. Thus, this study aimed to investigate the impact of various commercially available devices on transnasal aerosol delivery. METHODS: This was a bench study that used a 2-chamber lung model, in which one chamber was connected to an adult manikin with anatomically correct upper-airway proportions. The other chamber was connected to a critical care ventilator used to simulate spontaneous breathing. A size large nasal cannula was placed at the nasal opening of the manikin. Five different HFNC devices (Hamilton-C1, OptiFlow, Airvo2, V60 Plus, and Vapotherm) were compared. Four flow settings were used on each device, with a vibrating mesh nebulizer placed at the humidifier. Salbutamol (2.5 mg/3 mL) was used during the experiments to quantify inhaled drug doses. A collection filter was placed between the manikin's trachea and the lung model. The drug was eluted from the filter and assayed with ultraviolet spectrophotometry (276 nm). RESULTS: Among the 5 HFNC devices, OptiFlow had the highest inhaled dose at 10 L/min (mean ± SD 18.2% ± 1.2%). At 20 L/min, the Hamilton-C1 (mean ± SD 13.5% ± 0.4%) performed marginally better than the OptiFlow (mean ± SD 12.6% ± 1.9%) and Airvo2 (mean ± SD 12.8% ± 1%). At high flow settings (40--60 L/min), the inhaled dose of Hamilton-C1 was 2--3 times that of the Airvo2 and V60 Plus. When compared with the other devices, the mean inhaled dose with the Vapotherm was lower (0.9-2.5%). In all devices, the inhaled dose decreased as the flow increased. CONCLUSIONS: Transnasal aerosol delivery was significantly impacted by the types of HFNC devices and flow settings. Nominal doses might need to be adjusted if changing HFNC devices or flow is not an option. [ABSTRACT FROM AUTHOR]
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- 2023
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37. Comparison of the effects of ketamine via nebulization versus different pharmacological approaches in pediatric sedation: a systematic review and meta-analysis of randomized controlled trials.
- Author
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Liu, Xiao, Lang, Bingchen, Zeng, Linan, Huang, Liang, Chen, Shouming, Jia, Zhi-Jun, Cheng, Guo, Yu, Qin, and Zhang, Lingli
- Subjects
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ONLINE information services , *MEDICAL databases , *ANESTHESIA , *META-analysis , *MEDICAL information storage & retrieval systems , *CONFIDENCE intervals , *COMBINATION drug therapy , *SYSTEMATIC reviews , *PEDIATRICS , *AGITATION (Psychology) , *NEBULIZERS & vaporizers , *TREATMENT effectiveness , *RANDOMIZED controlled trials , *IMIDAZOLES , *KETAMINE , *DESCRIPTIVE statistics , *RESEARCH funding , *MEDLINE , *DRUG side effects , *PATIENT safety , *EVALUATION - Abstract
Background: Nebulized drug delivery is commonly used in pediatric clinical practice. The growing number of literatures have reported the application of nebulized ketamine in pediatric sedation in recent years. This meta-analysis of randomized controlled trials comparing the efficacy and safety of nebulized ketamine versus different pharmacological approaches was conducted to estimate the effects of this technique in pediatric sedation. Methods: We searched PubMed, Embase, and Cochrane Library from inception to Feb 2023. All randomized controlled trials used nebulized ketamine as presurgical and pre-procedural sedatives in children were included. Sedative effects and various adverse events were considered as the outcomes. Results: Ten studies with 727 pediatric patients were enrolled. Compared to nebulized dexmedetomidine, using of ketamine via nebulization showed similar sedation satisfaction (54.79% vs. 60.69%, RR = 0.88, with 95%CI [0.61, 1.27]), success rate of parental separation (57.27% vs. 73.64%, RR = 0.81, with 95%CI [0.61, 1.08]), and mask acceptability (37.27% vs. 52.73%, RR = 0.71, with 95%CI [0.45, 1.10]). However, the using of combination of two medications (nebulized ketamine plus nebulized dexmedetomidine) was associated with better sedative satisfaction (33.82% vs. 68.11%, RR = 0.50, with 95%CI [0.27, 0.92]) and more satisfactory mask acceptance (45.59% vs. 71.01%, RR = 0.69, with 95%CI [0.56, 0.86]). Compared with nebulized ketamine, using of nebulized dexmedetomidine was associated with less incidence of emergence agitation (18.18% vs. 3.33%, RR = 4.98, with 95%CI [1.88, 13.16]). Conclusions: Based on current evidences, compared to nebulized dexmedetomidine, nebulized ketamine provides inconspicuous advantages in pediatric sedation, and it has a relatively high incidence of emergence agitation. Combination of nebulized ketamine and dexmedetomidine might be considered as one preferred option in pediatric sedation as it can provide more satisfactory sedative effects. However, there is insufficient evidence regarding nebulized ketamine versus ketamine administered through other routes and nebulized ketamine versus other sedatives. The overall low or moderate quality of evidence evaluated by the GRADE system also calls for more high-quality studies with larger sample sizes in future. Research registration: The protocol of present study was registered with PROSPERO (CRD42023403226). [ABSTRACT FROM AUTHOR]
- Published
- 2023
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38. Engineered lipid liquid crystalline nanoparticles as an inhaled nanoplatform for mucus penetration enhancement.
- Author
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Wang, Wenhao, Zeng, Jingxuan, Luo, Peili, Fang, Jiayi, Pei, Qingao, Yan, Jinwu, Zhu, Chune, Chen, Wei, Liu, Yanyun, Huang, Zhengwei, Huang, Ying, Wu, Chuanbin, and Pan, Xin
- Abstract
Nanocarrier-assisted pulmonary drug delivery system has been widely employed for lung local disease treatment due to its enhanced drug lesion accumulation and reduced systematical side effects. However, the mucus barriers covered on the epithelia of trachea and bronchial tree construct a dense barrier for inhaled nanocarrier transport, which compromises the therapeutical effects. In this study, a lipid liquid crystalline nanoparticle NLP@Z with surface zwitterion material hexadecyl betaine (HB) modification and N-acetylcysteine (NAC) encapsulation was presented to exert the combination strategy of mucus-inert surface and mucus degradation. The HB modification endowed NLP@Z mucus-inert surface to inhibit the interaction between NLP@Z and mucins, and the encapsulated NAC could effectively degrade the mucins and further decrease the mucus viscosity. This combination strategy was proved to significantly promote the mucus penetration performance and enhance epithelial cell uptake. In addition, the proposed NLP@Z was equipped with desired nebulization property, which could be served as a potential pulmonary delivery nanoplatform. In summary, the proposed NLP@Z highlights the employment of the combination strategy for mucus penetration enhancement in pulmonary delivery, which may become a versatile platform for lung disease therapy. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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39. The effect of electrostatic high pressure nebulization on the stability, activity and ex vivo distribution of ionic self-assembled nanomedicines.
- Author
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Braet, Helena, Andretto, Valentina, Mariën, Remco, Yücesan, Beyza, van der Vegte, Stefan, Haegebaert, Ragna, Lollo, Giovanna, De Smedt, Stefaan C., and Remaut, Katrien
- Subjects
SPRAY nozzles ,NANOMEDICINE ,PERITONEAL cancer ,ELECTROSTATIC precipitation ,DRUG carriers ,PERITONEUM ,ELECTROSTATIC interaction - Abstract
Pressurized intraperitoneal aerosol chemotherapy (PIPAC) is applied to treat unresectable peritoneal metastasis (PM), an advanced, end-stage disease with a poor prognosis. Electrostatic precipitation of the aerosol (ePIPAC) is aimed at improving the intraperitoneal (IP) drug distribution and tumor penetration. Also, the combination of nanoparticles (NPs) as drug delivery vehicles and IP aerosolization as administration method has been proposed as a promising tool to treat PM. There is currently limited knowledge on how electrostatic precipitation (ePIPAC) and high pressure nebulization (PIPAC) affects the performance of electrostatically formed complexes. Therefore, the stability, in vitro activity and ex vivo distribution and tissue penetration of negatively charged cisPt-pArg-HA NPs and positively charged siRNA-RNAiMAX NPs was evaluated following PIPAC and ePIPAC. Additionally, a multidirectional Medspray® nozzle was developed and compared with the currently used Capnopen® nozzle. For both NP types, PIPAC and ePIPAC did not negatively influence the in vitro activity, although limited aggregation of siRNA-RNAiMAX NPs was observed following nebulization with the Capnopen®. Importantly, ePIPAC was linked to a more uniform distribution and higher tissue penetration of the NPs aerosolized by both nozzles, independent on the NPs charge. Finally, compared to the Capnopen®, an increased NP deposition was observed at the top of the ex vivo model following aerosolization with the Medspray® nozzle, which indicates that this device possesses great potential for IP drug delivery purposes. Aerosolized drug delivery in the peritoneal cavity holds great promise to treat peritoneal cancer. In addition, electrostatic precipitation of the aerosol to the peritoneal tissue is aimed at improving the drug distribution and tumor penetration. The combination of nanoparticles (NPs), which are nano-sized drug delivery vehicles, and aerosolization has been proposed as a promising tool to treat peritoneal cancer. However, there is currently limited knowledge on how electrostatic precipitation and aerosolization affect the performance of electrostatically formed NPs. Therefore, the stability, activity, distribution and penetration of negatively and positively charged NPs was evaluated after aerosolization and electrostatic precipitation. Additionally, to further optimize the local drug distribution, a multidirectional spray nozzle was developed and compared with the currently used nozzle. [Display omitted] [ABSTRACT FROM AUTHOR]
- Published
- 2023
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40. There Is a Risk of Spread During a Nebulization Session in a Patient with COVID-19.
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Thibon, Christophe, Vecellio, Laurent, Belkhir, Leila, Dubus, Jean-Christophe, Robert, Annie, Kabamba, Benoît, and Reychler, Gregory
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- *
COVID-19 , *AIRBORNE infection , *COVID-19 pandemic , *MICROBIOLOGICAL aerosols , *SARS-CoV-2 - Abstract
Introduction: A hypothetical risk of SARS-CoV-2 airborne transmission through nebulization was suggested based on a potential environmental contamination by the fugitive aerosol emitted in the environment during the procedure. The aim of this study was to verify this risk from the fugitive aerosol emitted by COVID-19 patients during one nebulization session. Methods: In this cohort study, COVID-19 patients treated with nebulization were recruited at their admission to the hospital. Patients had to perform a nebulization session while a BioSampler® and a pump were used to vacuum the fugitive aerosol and collect it for SARS-CoV-2 RNA detection. Results: Ten consecutive patients hospitalized with COVID-19 were recruited. The median viral load was 6.5 × 106 copies/mL. Two out of the 10 samples from the fugitive aerosol collected were positive to SARS-CoV-2. Conclusion: The risk of fugitive aerosol contamination with SARS-CoV-2 during nebulization has now been verified. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
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41. Aerosol Delivery of a Novel Recombinant Modified Superoxide Dismutase Protein Reduces Oxidant Injury and Attenuates Escherichia coli Induced Lung Injury in Rats.
- Author
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McCarthy, Sean D., Tilbury, Maura A., Masterson, Claire H., MacLoughlin, Ronan, González, Héctor E., Laffey, John G., Wall, J. Gerard, and O'Toole, Daniel
- Subjects
- *
SUPEROXIDE dismutase , *LUNG injuries , *PULMONARY alveolar proteinosis , *ADULT respiratory distress syndrome , *ESCHERICHIA coli , *CHIMERIC proteins - Abstract
Background: Acute respiratory distress syndrome (ARDS) is a life-threatening respiratory failure syndrome with diverse etiologies characterized by increased permeability of alveolar-capillary membranes, pulmonary edema, and acute onset hypoxemia. During the ARDS acute phase, neutrophil infiltration into the alveolar space results in uncontrolled release of reactive oxygen species (ROS) and proteases, overwhelming antioxidant defenses and causing alveolar epithelial and lung endothelial injury. Objectives: To investigate the therapeutic potential of a novel recombinant human Cu-Zn-superoxide dismutase (SOD) fusion protein in protecting against ROS injury and for aerosolized SOD delivery to treat Escherichia coli induced ARDS. Methods: Fusion proteins incorporating human Cu-Zn-SOD (hSOD1), with (pep1-hSOD1-his) and without (hSOD1-his) a fused hyaluronic acid-binding peptide, were expressed in E. coli. Purified proteins were evaluated in in vitro assays with human bronchial epithelial cells and through aerosolized delivery to the lung of an E. coli-induced ARDS rat model. Results: SOD proteins exhibited high SOD activity in vitro and protected bronchial epithelial cells from oxidative damage. hSOD1-his and pep1-hSOD1-his retained SOD activity postnebulization and exhibited no adverse effects in the rat. Pep1-hSOD1-his administered through instillation or nebulization to the lung of an E. coli-induced pneumonia rat improved arterial oxygenation and lactate levels compared to vehicle after 48 hours. Static lung compliance was improved when the pep1-hSOD1-his protein was delivered by instillation. White cell infiltration to the lung was significantly reduced by aerosolized delivery of protein, and reduction of cytokine-induced neutrophil chemoattractant-1, interferon-gamma, and interleukin 6 pro-inflammatory cytokine concentrations in bronchoalveolar lavage was observed. Conclusions: Aerosol delivery of a novel recombinant modified SOD protein reduces oxidant injury and attenuates E. coli induced lung injury in rats. The results provide a strong basis for further investigation of the therapeutic potential of hSOD1 in the treatment of ARDS. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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42. Application of instantaneous nebulization dispersive liquid‐phase microextraction combined with HPLC for the determination of chalcone and isoflavone in traditional Chinese medicines.
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Wang, Ling‐Li, Liu, Na, Hu, Shuang, Xing, Rong‐Rong, Wang, Run‐Qin, Yang, Li, and Chen, Xuan
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- *
CHINESE medicine , *HIGH performance liquid chromatography , *ORGANIC solvents , *ISOFLAVONES , *CHALCONE , *STANDARD deviations - Abstract
A simple and rapid instantaneous nebulization dispersive liquid‐phase microextraction method was developed, and combined with high‐performance liquid chromatography for determination of the contents of seven analytes in traditional Chinese medicines. In this study, using the sprinkler device to achieve instantaneous synchronous dispersion and extraction, only one spray can rapidly achieve the concentration and enrichment of seven kinds of chalcone and isoflavones. The key factors affecting the extraction efficiency were optimized including the type and volume of extractant, the pH and salt concentration of the sample phase, and the number of dispersion. Under the optimal conditions, the enrichment factor of the target analytes ranged from 103.1 to 180.9, with good linearity and correlation coefficients above 0.9970. The limits of detection ranged from 0.02 to 0.15 ng/mL, with good accuracy (recoveries 91.1 to 108.9%) and precision (relative standard deviations 1.5–7.1%). This method has short extraction time (2 s), low organic solvent consumption and high enrichment effect, so it has a wide application prospects. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
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43. Smart hydrogels delivered by high pressure aerosolization can prevent peritoneal adhesions.
- Author
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Braet, Helena, Fransen, Peter-Paul, Chen, Yong, Van Herck, Simon, Mariën, Remco, Vanhoorne, Valérie, Ceelen, Wim, Madder, Annemieke, Ballet, Steven, Hoogenboom, Richard, De Geest, Bruno, Hoorens, Anne, Dankers, Patricia Y.W., De Smedt, Stefaan C., and Remaut, Katrien
- Subjects
- *
TISSUE adhesions , *THERMORESPONSIVE polymers , *POLYMER solutions , *PERITONEUM , *ADHESION , *GELATION , *HYDROGELS , *BIOMATERIALS - Abstract
Postoperative peritoneal adhesions occur in the majority of patients undergoing intra-abdominal surgery and are one of the leading causes of hospital re-admission. There is an unmet clinical need for effective anti-adhesive biomaterials, which can be applied evenly across the damaged tissues. We examined three different responsive hydrogel types, i.e. a thermosensitive PLGA-PEG-PLGA, a pH responsive UPy-PEG and a shear-thinning hexapeptide for this purpose. More specifically, their potential to be homogeneously distributed in the peritoneal cavity by high pressure nebulization and prevent peritoneal adhesions was evaluated. Solutions of each polymer type could be successfully nebulized while retaining their responsive gelation behavior in vitro and in vivo. Furthermore, none of the polymers caused in vitro toxicity on SKOV3-IP2 cells. Following intraperitoneal administration, both the PLGA-PEG-PLGA and the hexapeptide hydrogels resulted in local inflammation and fibrosis and failed in preventing peritoneal adhesions 7 days after adhesion induction. In contrast, the pH sensitive UPy-PEG formulation was well tolerated and could significantly reduce the formation of peritoneal adhesions, even outperforming the commercially available Hyalobarrier® as positive control. To conclude, local nebulization of the bioresponsive UPy-PEG hydrogel can be considered as a promising approach to prevent postsurgical peritoneal adhesions. [Display omitted] [ABSTRACT FROM AUTHOR]
- Published
- 2023
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44. Inhalation with Vitamin D3 Metabolites—A Novel Strategy to Restore Vitamin D3 Deficiencies in Lung Tissue.
- Author
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Chojnacki, Michał, Anisiewicz, Jakub, Leśniowska, Ilona, and Lemieszek, Marta Kinga
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CHOLECALCIFEROL ,VITAMIN deficiency ,VITAMIN D ,DIETARY supplements ,VITAMIN D deficiency ,LUNGS - Abstract
Vitamin D3 deficiency has been recognized as a pandemic with serious health consequences including chronic respiratory diseases. Unfortunately, improvement in this situation by using vitamin D supplementation has failed. The direct delivery of 1,25(OH)
2 -vitamin D3 and its precursor into the respiratory tract, by nebulization, seemed to be a better option, as verified in the presented study. To induce vitamin D deficiency, mice received a diet with 0.05 IU/g cholecalciferol, while control animals were given feed with 0.5 IU/g cholecalciferol. Vitamin-D-deficient mice were exposed to different doses of calcidiol or calcitriol via nebulization for at least 7 days. At the end of the experiment, whole-body plethysmography was conducted. Pulmonary and serum levels of calcitriol were examined using ELISA. The calcitriol concentrations in mice on standard vs. deficient diet were 30.31/18.20 pg/mg (lungs) and 132.24/98.61 pg/mL (serum), respectively. Restoration of the physiological level of calcitriol in vitamin-D-deficient mice required 1-week exposure to 100 pg/g of calcidiol or 5 pg/g of calcitriol. The inhalations did not cause any side changes in murine respiratory function. The presented study revealed the usefulness and safety of chronic inhalation with a bioactive form of vitamin D3 or its precursor for the restoration of physiological calcitriol levels in animals with vitamin D deficiencies. [ABSTRACT FROM AUTHOR]- Published
- 2023
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45. Inhaled 'Muco‐Trapping' Monoclonal Antibody Effectively Treats Established Respiratory Syncytial Virus (RSV) Infections
- Author
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Morgan D. McSweeney, Sarhad Alnajjar, Alison M. Schaefer, Zach Richardson, Whitney Wolf, Ian Stewart, Pun Sriboonyapirat, Justin McCallen, Ellen Farmer, Bernadette Nzati, Sam Lord, Brian Farrer, Thomas R. Moench, Priya A. Kumar, Harendra Arora, Raymond J. Pickles, Anthony J. Hickey, Mark Ackermann, and Samuel K. Lai
- Subjects
mAb nebulization ,monoclonal antibody ,nebulization ,respiratory syncytial virus ,RSV ,Science - Abstract
Abstract Respiratory syncytial virus (RSV) causes substantial morbidity and mortality in infants, the immunocompromised, and the elderly. RSV infects the airway epithelium via the apical membrane and almost exclusively sheds progeny virions back into the airway mucus (AM), making RSV difficult to target by systemically administered therapies. An inhalable “muco‐trapping” variant of motavizumab (Mota‐MT), a potent neutralizing mAb against RSV F is engineered. Mota‐MT traps RSV in AM via polyvalent Fc‐mucin bonds, reducing the fraction of fast‐moving RSV particles in both fresh pediatric and adult AM by ≈20–30‐fold in a Fc‐glycan dependent manner, and facilitates clearance from the airways of mice within minutes. Intranasal dosing of Mota‐MT eliminated viral load in cotton rats within 2 days. Daily nebulized delivery of Mota‐MT to RSV‐infected neonatal lambs, beginning 3 days after infection when viral load is at its maximum, led to a 10 000‐fold and 100 000‐fold reduction in viral load in bronchoalveolar lavage and lung tissues relative to placebo control, respectively. Mota‐MT‐treated lambs exhibited reduced bronchiolitis, neutrophil infiltration, and airway remodeling than lambs receiving placebo or intramuscular palivizumab. The findings underscore inhaled delivery of muco‐trapping mAbs as a promising strategy for the treatment of RSV and other acute respiratory infections.
- Published
- 2024
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46. Histological Assessment of Respiratory Tract and Liver of BALB/c Mice Nebulized with Tocilizumab
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Paloma Jimena de Andres, Sergio Ferreiro, Angela Flores, Almudena Garcia, and Cesar Henriquez-Camacho
- Subjects
nebulization ,animal model ,monoclonal antibody ,tocilizumab ,BALB/c ,Pharmacy and materia medica ,RS1-441 - Abstract
Pulmonary drug delivery offers a minimally invasive and efficient method for treating lung conditions, leveraging the lungs’ extensive surface area and blood flow for rapid drug absorption. Nebulized therapies aim to deliver drugs directly to the lung tissue. This study investigates the histological impact of nebulized tocilizumab—a monoclonal antibody targeting IL-6, traditionally administered intravenously for rheumatoid arthritis and severe COVID-19—on a murine model. Thirty BALB/c mice were nebulized with tocilizumab (10 mg, 5 mg, and 2.5 mg) and six controls were nebulized with saline solution. They were euthanized 48 h later, and their organs (lungs, nasal mucosa, and liver) were analyzed by a microscopic histological evaluation. The results indicate that all the mice survived the 48 h post-nebulization period without systemic compromise. The macroscopic examination showed no abnormalities, and the histopathological analysis revealed greater lung vascular changes in the control group than in the nebulized animals, which is attributable to the euthanasia with carbon dioxide. Additionally, increased alveolar macrophages were observed in the nebulized groups compared to controls. No significant histological changes were observed in the liver, indicating the safety of nebulized tocilizumab. In conclusion, these findings suggest the potential of nebulized tocilizumab for treating pulmonary inflammation, warranting further research to establish its efficacy and safety in clinical settings.
- Published
- 2024
- Full Text
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47. Solid Lipid Nanoparticles Loaded with Dexamethasone Palmitate for Pulmonary Inflammation Treatment by Nebulization Approach
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Hsin-Hung Chen, Chen-Hsiang Sang, Chang-Wei Chou, Yi-Ting Lin, Yi-Shou Chang, and Hsin-Cheng Chiu
- Subjects
anti-inflammation ,dexamethasone palmitate ,nebulization ,pneumonia ,solid lipid nanoparticles ,alveolar macrophages ,Pharmacy and materia medica ,RS1-441 - Abstract
Pneumonia stands as the leading infectious cause of childhood mortality annually, underscoring its significant impact on pediatric health. Although dexamethasone (DXMS) is effective for treating pulmonary inflammation, its therapeutic potential is compromised by systemic side effects and suboptimal carrier systems. To address this issue, the current study introduces solid lipid nanoparticles encapsulating hydrophobic dexamethasone palmitate (DXMS-Pal-SLNs) as an anti-inflammatory nanoplatform to treat pneumonia. The specialized nanoparticle formulation is characterized by high drug loading efficiency, low drug leakage and excellent colloidal stability in particular during nebulization and is proficiently designed to target alveolar macrophages in deep lung regions via local delivery with the nebulization administration. In vitro analyses revealed substantial reductions in the secretions of tumor necrosis factor-α and interleukin-6 from alveolar macrophages, highlighting the potential efficacy of DXMS-Pal-SLNs in alleviating pneumonia-related inflammation. Similarly, in vivo experiments showed a significant reduction in the levels of these cytokines in the lungs of mice experiencing lipopolysaccharide-induced pulmonary inflammation after the administration of DXMS-Pal-SLNs via nebulization. Furthermore, the study demonstrated that DXMS-Pal-SLNs effectively control acute infections without causing pulmonary infiltration or excessive recruitment of immunocytes in lung tissues. These findings highlight the potential of nebulized DXMS-Pal-SLNs as a promising therapeutic strategy for mitigating pneumonia-related inflammations.
- Published
- 2024
- Full Text
- View/download PDF
48. The effect of various inhaled asthma medications on the color stability of paediatric dental restorative materials
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Candan, Merve and Ünal, Murat
- Published
- 2024
- Full Text
- View/download PDF
49. Nebulized Recombinant Tissue Plasminogen Activator (rt-PA) for Acute COVID-19-Induced Respiratory Failure: An Exploratory Proof-of-Concept Trial.
- Author
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Chowdary, Pratima, Agarwal, Banwari, Peralta, Maria Rita, Bhagani, Sanjay, Lee, Simon, Goldring, James, Lipman, Marc, Waqif, Emal, Phillips, Mark, Philippou, Helen, Foley, Jonathan H., Mutch, Nicola J., Ariëns, Robert A. S., Stringer, Kathleen A., Ricciardi, Federico, Watissée, Marie, Hughes, Derralynn, Nathwani, Amit, Riddell, Anne, and Patch, David
- Subjects
- *
TISSUE plasminogen activator , *PROOF of concept , *CLINICAL trials , *NEAR infrared spectroscopy - Abstract
Acute lung injury in COVID-19 results in diffuse alveolar damage with disruption of the alveolar-capillary barrier, coagulation activation, alveolar fibrin deposition and pulmonary capillary thrombi. Nebulized recombinant tissue plasminogen activator (rt-PA) has the potential to facilitate localized thrombolysis in the alveolar compartment and improve oxygenation. In this proof-of-concept safety study, adults with COVID-19-induced respiratory failure and a <300 mmHg PaO2/FiO2 (P/F) ratio requiring invasive mechanical ventilation (IMV) or non-invasive respiratory support (NIRS) received nebulized rt-PA in two cohorts (C1 and C2), alongside standard of care, between 23 April–30 July 2020 and 21 January–19 February 2021, respectively. Matched historical controls (MHC; n = 18) were used in C1 to explore efficacy. Safety co-primary endpoints were treatment-related bleeds and <1.0–1.5 g/L fibrinogen reduction. A variable dosing strategy with clinical efficacy endpoint and minimal safety concerns was determined in C1 for use in C2; patients were stratified by ventilation type to receive 40–60 mg rt-PA daily for ≤14 days. Nine patients in C1 (IMV, 6/9; NIRS, 3/9) and 26 in C2 (IMV, 12/26; NIRS, 14/26) received nebulized rt-PA for a mean (SD) of 6.7 (4.6) and 9.1(4.6) days, respectively. Four bleeds (one severe, three mild) in three patients were considered treatment related. There were no significant fibrinogen reductions. Greater improvements in mean P/F ratio from baseline to study end were observed in C1 compared with MHC (C1; 154 to 299 vs. MHC; 154 to 212). In C2, there was no difference in the baseline P/F ratio of NIRS and IMV patients. However, a larger improvement in the P/F ratio occurred in NIRS patients (NIRS; 126 to 240 vs. IMV; 120 to 188) and fewer treatment days were required (NIRS; 7.86 vs. IMV; 10.5). Nebulized rt-PA appears to be well-tolerated, with a trend towards improved oxygenation, particularly in the NIRS group. Randomized clinical trials are required to demonstrate the clinical effect significance and magnitude. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
50. Stability Considerations for Bacteriophages in Liquid Formulations Designed for Nebulization.
- Author
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Flint, Rohan, Laucirica, Daniel R., Chan, Hak-Kim, Chang, Barbara J., Stick, Stephen M., and Kicic, Anthony
- Subjects
- *
CHRONIC obstructive pulmonary disease , *BACTERIOPHAGES , *CYSTIC fibrosis , *LUNG infections , *RESPIRATORY infections , *DRUG resistance in microorganisms - Abstract
Pulmonary bacterial infections present a significant health risk to those with chronic respiratory diseases (CRDs) including cystic fibrosis (CF) and chronic-obstructive pulmonary disease (COPD). With the emergence of antimicrobial resistance (AMR), novel therapeutics are desperately needed to combat the emergence of resistant superbugs. Phage therapy is one possible alternative or adjunct to current antibiotics with activity against antimicrobial-resistant pathogens. How phages are administered will depend on the site of infection. For respiratory infections, a number of factors must be considered to deliver active phages to sites deep within the lung. The inhalation of phages via nebulization is a promising method of delivery to distal lung sites; however, it has been shown to result in a loss of phage viability. Although preliminary studies have assessed the use of nebulization for phage therapy both in vitro and in vivo, the factors that determine phage stability during nebulized delivery have yet to be characterized. This review summarizes current findings on the formulation and stability of liquid phage formulations designed for nebulization, providing insights to maximize phage stability and bactericidal activity via this delivery method. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
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