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Diethyl aminoethyl hexanoate (DA-6) serving as a non-toxic and low-cost plant growth regulator is used for improving plant growth and stress tolerance, but the DA-6-mediated organic metabolites remodeling in relation to drought tolerance is not well documented in crops. The aims of the present study were to evaluate impacts of DA-6 on physiological functions including osmotic adjustment, photochemical efficiency, oxidative damage, and cell membrane stability as well as organic metabolites remodeling in white clover (Trifolium repens) leaves based on the analysis of metabolomics. Plants were foliarly treated with or without DA-6 and subsequently exposed to drought stress for 8 days. Results demonstrated that foliar application of DA-6 (1.5 mM) could significantly ameliorate drought tolerance, which was linked with better leaf water status, photosynthetic performance, and cell membrane stability as well as lower oxidative injury in leaves. Metabolic profiling of organic metabolites identified a total of 59 metabolites including 17 organic acids, 20 sugars, 12 alcohols, and 10 other metabolites. In response to drought stress, the DA-6 induced accumulations of many sugars and sugar alcohols (erythrulose, arabinose, xylose, inosose, galactose, talopyranose, fucose, erythritol, and ribitol), organic acids (propanoic acid, 2,3-dihydroxybutanoic acid, palmitic acid, linolenic acid, and galacturonic acid), and other metabolites (2-oxazoline, silane, and glycine) in white clover. These altered metabolites induced by the DA-6 could perform critical functions in maintenances of osmo-protection, osmotic adjustment, redox homeostasis, cell wall structure and membrane stability when white clover suffered from water deficit. In addition, the campesterol and stigmasterol significantly accumulated in all plants in spite of the DA-6 pretreatment under drought stress, which could be an important adaptive response to water deficit due to beneficial roles of those two metabolites in regulating cell membrane stability and antioxidant defense. Present findings provide new evidence of DA-6-regulated metabolic homeostasis contributing to drought tolerance in leguminous plants. [ABSTRACT FROM AUTHOR]

This research paper investigates the challenges encountered by Bachelor of Science in English (BS English) students in analyzing literature. It focuses on difficulties such as recognizing symbolism, understanding historical context, identifying literary devices, grasping narrative voice, interpreting stage directions, discerning intended meanings, and analyzing plot structure. A mixed-methods approach was employed, involving a comprehensive literature review and qualitative data collection through questionnaire conducted among BS English students. The literature review provided a theoretical framework for understanding the complexities of literary analysis, while the qualitative data collection offered insights into the practical experiences and perceptions of students. The findings reveal several noteworthy trends. Students often struggle to recognize symbolism, frequently misinterpreting or overlooking its significance in literary texts. Understanding the historical context of works proves challenging, with many students failing to connect the socio-political climate of the author's time with the themes of the text. Based on these findings, recommendations are proposed to investigate how cultural background influence the challenges encountered by BS English students in literature analysis, considering factors such as language proficiency and exposal to diverse literary traditions. [ABSTRACT FROM AUTHOR]