95 results on '"Mohammad Javed Ali"'
Search Results
2. Pediatric dacryolith masquerading as congenital nasolacrimal duct obstruction
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Upasana Pokal, Ashish Ranjan, and Mohammad Javed Ali
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Dacryolith ,Lacrimal sac ,Lacrimal ,Congenital nasolacrimal duct obstruction ,Congenital ,Ophthalmology ,RE1-994 - Abstract
Purpose: To report a rare case of a pediatric dacryolith masquerading as congenital nasolacrimal duct obstruction (CNLDO). Observations: A two-year-old male child presented with history of intermittent epiphora and discharge since the age of six months. Clinical evaluation demonstrated raised tear meniscus height and delayed fluorescein dye disappearance test in the right eye. Lacrimal irrigation of the right eye under general anesthesia demonstrated 90 % regurgitation (subjectively) of mucoid fluid with a hard stop. Nasal endoscopy examination demonstrated a dacryolith obstructing the opening of the nasolacrimal duct (NLD) in the inferior meatus. The dacryolith was teased out of the NLD and following its removal the lacrimal irrigation was freely patent. At six-months post operative follow up, epiphora resolved and the child was asymptomatic. Conclusions and importance: While cases of canaliculitis is uncommon in pediatric age group, it is rare to find a NLD dacryolith in a toddler. To the best of the authors’ knowledge, there are few prior reports on pediatric NLD dacryolith masquerading as CNLDO in a toddler (1–3 years).
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- 2024
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3. Generation of two induced pluripotent stem cell lines (LVPEIi004-A and LVPEIi005-A) from probands with Leber Congenital Amaurosis 2 (LCA2) and harboring mutations in RPE65
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Savitri Maddileti, Sudipta Mahato, Trupti Agrawal, Vivek Pravin Dave, Milind Naik, Mohammad Javed Ali, Chitra Kannabiran, Subhadra Jalali, Giridhara R. Jayandharan, and Indumathi Mariappan
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Biology (General) ,QH301-705.5 - Abstract
Leber Congenital Amaurosis 2 is an early onset retinal dystrophy that occurs due to mutation in RPE65 gene. Here, we report the generation of two patient specific induced pluripotent stem cell lines harboring nonsense mutations in exon 7 (c.646A > T) and exon 9 (c.992G > A) of RPE65 gene, respectively, which leads to premature translational termination and formation of defective protein. These lines were generated by the reprogramming of human dermal fibroblast cells using integration-free, episomal constructs expressing stemness genes. The stable lines maintained a normal karyotype, expressed the key stemness factors, underwent trilineage differentiation, and maintained their genetic identity and genomic integrity.
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- 2024
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4. The role of CT-DCG in hardware – associated secondary acquired lacrimal duct obstruction: SALDO update study - (SUP) - Paper III
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Ayushi Agarwal, Milind Naik, Mohammad Javed Ali, and Nandini Bothra
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Secondary acquired lacrimal duct obstruction ,SALDO ,Lacrimal duct obstruction ,Traumatic lacrimal duct obstruction ,Lacrimal ,Ophthalmology ,RE1-994 - Abstract
Purpose: To study cases of hardware-associated secondary acquired lacrimal duct obstructions (SALDO) and the role of computed tomography dacryocystography (CT-DCG) scans in its diagnosis and management. Observations: Retrospective, interventional case review of four patients diagnosed as Hardware-associated SALDO, with primary maxillofacial repair performed elsewhere, were analyzed. The mean age was 36.5 years (range: 22–74 years), three of them being males. Left and right lacrimal systems were equally involved. Epiphora, swelling and discharge were the main presenting features. Three lacrimal sacs and nasolacrimal ducts on CT-DCG showed the screws of the orbital fracture plate directly piercing their walls, whereas a single case showed the sac displaced and pierced by the medial side of the orbital floor implant. Two cases underwent dacryocystorhinostomy with intubation, and the remaining two had to undergo dacryocystectomy due to extensive damage. Post-operatively all four cases were doing well at a mean follow-up of 2 months. Conclusions: The present series highlights the role of CT-DCG in delineating spatial relationship of lacrimal drainage system with the surrounding structures, facilitating planning as well as choice of surgery, and anticipating the intraoperative challenges. It also signifies importance of interdisciplinary coordination between oculoplastic and maxillofacial surgeons to avoid iatrogenic trauma to the lacrimal drainage system.
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- 2024
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5. Early trends of secondary acquired nasolacrimal duct obstruction in COVID-19 associated sino-orbital mucormycosis: SALDO update study (SUP) – Paper IV
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Nandini Bothra, Yamini Priya, D. Rincy, and Mohammad Javed Ali
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Secondary acquired lacrimal duct obstruction ,SALDO ,Rhino-orbito-cerebral mucormycosis ,ROCM ,Lacrimal ,Ophthalmology ,RE1-994 - Abstract
Purpose: To study the emerging trend of development of secondary acquired nasolacrimal duct obstruction (SALDO) in the cases treated for rhino-orbito-cerebral mucormycosis (ROCM). Observations: Prospective, interventional case series of five patients who developed SALDO post-surgical and medical management of ROCM. The mean age was 45.4 years (range: 40–62 years), all five being males. Right lacrimal drainage pathways was involved slightly more than the left (right:left = 3:2). Epiphora and discharge were the main presenting features. All five patients were treated with extensive debridement of the nose, sinuses, orbit and cerebral involvement as needed followed by medical management for ROCM. CT- Dacryocystography for all 5 patients showed obstruction at the lower end of the nasolacrimal duct. The patients underwent external dacryocystorhinostomy with mitomycin-C with or without intubation with good anatomical and functional outcomes at 6 months follow-up. Conclusions and importance: The present series highlights the possible etiology of development of SALDO in cases treated for ROCM, its management and discusses the role of Computed Tomography Dacryocystography (CT-DCG) in understanding the pathophysiology of the development of SALDO along with delineating the spatial relationship of the lacrimal drainage system with the surrounding structures.
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- 2024
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6. Nasolacrimal duct coronary stent recanalization as a possible alternative to dacryocystorhinostomy
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Nandini Bothra and Mohammad Javed Ali
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Ophthalmology ,RE1-994 - Published
- 2023
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7. Scanning electron microscopic features of lacrimal drainage silastic stents: Comparison of various Crawford and large-diameter stents.
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Emmanuel Lee Ong Boniao, Alexander Gerard Nino L Gungab, Blanche Xiao Hong Lim, Gangadhara Sundar, and Mohammad Javed Ali
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Medicine ,Science - Abstract
PurposeThis study aimed to examine the differences in the biofilms and physical deposits on Crawford stents compared to large-diameter stents.MethodsA prospective interventional study was performed on a series of patients undergoing external or endoscopic dacryocystorhinostomy (DCR) and endoluminal lacrimal duct recanalization (ELDR) with either Crawford or large-diameter stents. All the Crawford stents were retrieved at six weeks and the large-diameter ones at eight weeks following the surgical intervention. There was no evidence of post-operative infection in any of the patients. Following extubation, standard protocols of scanning electron microscopy were used to assess the biofilms and physical deposits on the stents.ResultsA total of 15 stents were studied. Of these, twelve were Crawford, and three were large-diameter stents. The Crawford stents were from two different manufacturers. All the stents demonstrated evidence of biofilm formation and physical deposits. The Crawford stents showed thin biofilms and sparse physical deposits, but there were no demonstrable differences amongst stents from different manufacturers. However, the deposits and biofilms were thicker and more extensive in the large-diameter stents than the Crawford ones. The biofilms from all stents showed the presence of polymicrobial communities within the exopolysaccharide matrix.ConclusionsThe present study found differences in biofilms and physical deposits between Crawford and large-diameter stents. These differences can be partly explained by stent duration, size, and their tissue interactions.
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- 2023
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8. Neutrophil Extracellular Traps Drive Dacryolithiasis
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Leticija Zlatar, Thomas Timm, Günter Lochnit, Rostyslav Bilyy, Tobias Bäuerle, Marco Munoz-Becerra, Georg Schett, Jasmin Knopf, Jens Heichel, Mohammad Javed Ali, Mirco Schapher, Friedrich Paulsen, and Martin Herrmann
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mucopeptide concretions ,dacryoliths ,dacryolithiasis ,lacrimal sac ,neutrophils ,neutrophil extracellular traps ,Cytology ,QH573-671 - Abstract
Mucopeptide concretions, previously called dacryoliths, are macroscopic stones that commonly obstruct the lacrimal sac. The mechanism behind dacryolithiasis remains unclear; however, the involvement of various immune cells, including neutrophils, has been confirmed. These findings remain limited, and no information on neutrophil extracellular traps (NETs), essentially involved in the pathogenesis of other lithiases, is available yet. Here, we employ microcomputed tomography, magnetic resonance tomography, histochemistry, mass spectrometry, and enzyme activity analyses to investigate the role of neutrophils and NETs in dacryolithiasis. We classify mucopeptide concretions into three types, with respect to the quantity of cellular and acellular material, polysaccharides, and mucosubstances. We propose the role of neutrophils and NETs within the existing model of gradual formation and growth of mucopeptide concretions, with neutrophils contributing to the initial stages of dacryolithiasis, as they localized on the inner (older) parts of the tissue. As NETs localized on the outer (newer) parts of the tissue, we link their role to the late stages of dacryolithiasis, presumably maintaining the proinflammatory environment and preventing efficient clearance. An abundance of IgG on the surface indicates the involvement of the adaptive immune system later as well. These findings bring new perspectives on dacryolithiasis, in which the innate and adaptive immune system are essentially involved.
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- 2023
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9. Holding back the tears: does marsupialisation of a remnant canaliculus after tumour resection help eliminate epiphora?
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Raman Malhotra, Geoffrey E Rose, Bhupendra C K Patel, and Mohammad Javed Ali
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Ophthalmology ,RE1-994 - Published
- 2022
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10. No room for ambiguity: The concepts of appropriate and inappropriate authorship in scientific publications
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Mohammad Javed Ali
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authorship ,committee on publication ethics ,council for science editors ,ethics ,international committee of medical journal editors ,publication ,Ophthalmology ,RE1-994 - Abstract
Authorship is the currency of an academic career. Scientific publications have significant academic and financial implications. Several standard authorship guidelines exist, and the International Committee of Medical Journal Editors (ICMJE) is the most popular amongst them. There are increasing concerns about the ethics of publications with the rise of inappropriate authorship. The most important reason appears to be a lack of knowledge and awareness of the authorship guidelines and what actions constitute unethical behaviors. There is a need to incorporate standard guidelines in medical curricula and conduct structured training and education programs for researchers across the board. The current perspective describes the significant concepts of appropriate and inappropriate authorship, and the possible measures being formulated to shape the future of authorship.
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- 2021
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11. Severe ocular and adnexal complications in dengue hemorrhagic fever: A report of 29 eyes
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V S Vijitha, Tarjani Vivek Dave, Somasheila I Murthy, Mohammad Javed Ali, Vivek Pravin Dave, Rajeev Reddy Pappuru, and Raja Narayanan
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dengue ,necrosis ,panophthalmitis ,phthisis bulbi ,scleral melt ,Ophthalmology ,RE1-994 - Abstract
Purpose: To describe a series of sight-threatening ocular and adnexal involvement associated with dengue hemorrhagic fever and their treatment options. Methods: Retrospective, interventional, non-comparative case series. Medical records of patients who presented with ocular symptoms attributed to dengue hemorrhagic fever were reviewed. Demography, presenting features, and treatment outcomes were recorded. Outcome measures assessed included globe salvage, vision salvage, and visual improvement. The outcome was deemed as favorable if vision salvage was possible and unfavorable if the vision was lost. Results: Twenty-nine eyes of 23 patients were included. Bilateral ocular involvement was seen in 6 (26%) patients. The mean age was 37.62 ± 18.68 years (range: 14 to 81 years). Coexistent diabetes mellitus and enteric fever were present in three patients. History of blood transfusion was present in nine (40%) and thrombocytopenia in eight (35%) patients at the time of presentation with ocular complaints. Presenting features included endophthalmitis in 11 (38%), panophthalmitis in 10 (35%), orbital cellulitis with panophthalmitis in four (14%), isolated corneal or scleral melt in three (10%), and orbital hemorrhage with panophthalmitis in one (3%) patient. Globe salvage was achieved in 21/29 eyes (72.4%), vision salvage in 6/29 eyes (20.68%), and improvement in visual acuity was noted in 5/29 eyes (17.24%). Logistic regression analysis revealed no significant effect of any clinical-microbiological factors on globe salvage, vision salvage, and visual improvement. However, visual improvement and globe salvage were possible in eyes that underwent early endoscopic vitrectomy. Conclusion: Dengue fever can present with sight-threatening ocular and adnexal inflammation resulting in endophthalmitis and panophthalmitis, orbital cellulitis, corneal and scleral melt, and orbital hemorrhage. Early vitrectomy may improve vision and globe salvage in cases with significant vitritis.
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- 2021
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12. Long-term outcomes of revision endoscopic dacryocystorhinostomy aided by 4-mm coronary balloon catheter dacryoplasty
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Mohammad Javed Ali and Nandini Bothra
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balloon dacryoplasty ,dacryocystorhinostomy ,endoscopic dcr ,failed dcr ,lacrimal ,Ophthalmology ,RE1-994 - Abstract
Our purpose was assess the long-term efficacy of 4-mm coronary balloon catheter dacryoplasty in revision endoscopic dacryocystorhinostomy (RevEnDCR). This retrospective interventional case-series was performed for patients who underwent RevEnDCR aided by a 4-mm coronary balloon catheter (CBC) dacryoplasty. The indications for the surgery were previously failed DCRs by external or endoscopic approach where the ostium showed near total cicatrization with or without the presence of organized granuloma threatening the internal common opening (ICO). The coronary balloon (4 × 10 mm, SPALNO, Cardiomac, Haryana, India) with the guidewire was used and a minimum of >12 months of follow-up was considered for analysis. Ten lacrimal systems of eight patients with mean age of 48.8 years underwent CBC-assisted revision endoscopic DCR. Of the 10 failed DCRs, 6 had a previous external approach DCR and 4 were endoscopic DCRs. Grossly stenosed ostium with near total cicatricial closure were noted in half of the patients (50%, 5/10) while the remaining half, in addition, showed organized granulomas threatening the ICO. The surgical technique using CBC was found to be minimally invasive, easy to perform with multiple advantages like uniform clearance of the area in front of ICO and more predictable lacrimal sac flaps. At a mean follow-up of 20 months, anatomical and functional success were achieved in 90% (9/10) of the eyes. We conclude that coronary balloon catheter-assisted revision endoscopic DCR is a minimally invasive and viable alternative in select group of patients of failed DCR with near total cicatrisation or organized granulomas threatening ICO.
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- 2021
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13. Impact of Microplastics on the Ocular Surface
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Duoduo Wu, Blanche X. H. Lim, Ivan Seah, Shay Xie, Julia E. Jaeger, Robert K. Symons, Amy L. Heffernan, Emily E. M. Curren, Sandric C. Y. Leong, Andri K. Riau, Dawn K. A. Lim, Fiona Stapleton, Mohammad Javed Ali, Swati Singh, Louis Tong, Jodhbir S. Mehta, Xinyi Su, and Chris H. L. Lim
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microplastics ,ocular surface ,dry eye disease ,polymers ,dysbiosis ,oxidative stress ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Plastics are synthetic materials made from organic polymers that are ubiquitous in daily living and are especially important in the healthcare setting. However, recent advances have revealed the pervasive nature of microplastics, which are formed by degradation of existing plastic products. Although the impact on human health has yet to be fully characterised, there is increasing evidence that microplastics can trigger inflammatory damage, microbial dysbiosis, and oxidative stress in humans. Although there are limited studies investigating their effect on the ocular surface, studies of microplastics on other organs provide some insights. The prevalence of plastic waste has also triggered public outcry, culminating in the development of legislation aimed at reducing microplastics in commercial products. We present a review outlining the possible sources of microplastics leading to ocular exposure, and analyse the possible mechanisms of ocular surface damage. Finally, we examine the utility and consequences of current legislation surrounding microplastic regulation.
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- 2023
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14. COVID-19 pandemic and lacrimal practice: Multipronged resumption strategies and getting back on our feet
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Mohammad Javed Ali
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2019-ncov ,covid-19 ,dcr ,endoscopy ,lacrimal ,nasolacrimal duct ,personal protective equipment ,sars-cov-2 ,Ophthalmology ,RE1-994 - Abstract
The aim of this review was to propose multi-pronged resumption strategies for lacrimal practice in an effort to plan a sustainable recommencement of elective surgeries after we emerge from the peak of COVID-19 pandemic. The strategies for lacrimal practice were classified into 7 subtypes, and each of the blueprints were reassessed based on existing information on resumption strategies of elective surgeries from other specialties in COVID-19 era. The specific needs of lacrimal practice were then added to construct algorithms summarizing the resumption strategies. The basic principle of 'primum non nocere' needs to be followed. The overall proposed plan advocates the transition to a more sustainable health care reality in a world where we would still co-exist with COVID-19. A comprehensive effort involving screening, laboratory testing, appropriate triage, effective personal protection and specific precautionary measures for lacrimal clinics and operating room are needed to be able to safely resume elective surgery when the pandemic peak declines. To predict the timing of the resumption of elective surgeries is quite complex and influenced by several geographic, political and economic factors. It is equally important to remember that COVID-19 crisis is a dynamic situation and constantly evolving, hence the strategies provided are subject to change. Strict adherence to standard COVID-19 guidelines combined with effective testing and personal protection strategies can ensure slow yet smooth and safe return to full lacrimal practice after the COVID-19 pandemic calms down. The local government directives, individual and institutional discretion are advised.
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- 2020
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15. All India Ophthalmological Society - Oculoplastics Association of India consensus statement on preferred practices in oculoplasty and lacrimal surgery during the COVID-19 pandemic
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Mohammad Javed Ali, Raghuraj Hegde, Akshay Gopinathan Nair, Mandeep S Bajaj, Subhash M Betharia, Kasturi Bhattacharjee, Apjit K Chhabra, Jayanta K Das, Gagan Dudeja, Ashok K Grover, Santosh G Honavar, Usha Kim, Lakshmi Mahesh, Bipasha Mukherjee, Anita Sethi, Mukesh Sharma, and Usha Singh
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blepharoplasty ,botox ,corona ,coronavirus ,dacryocystorhinostomy ,dcr ,eyelid surgery ,guidelines ,lockdown ,ophthalmology ,orbital surgery ,precautions ,ptosis ,Ophthalmology ,RE1-994 - Abstract
Oculoplastic surgeries encompass both emergency surgeries for traumatic conditions and infectious disorders as well as elective aesthetic procedures. The COVID-19 pandemic has brought about a drastic change in this practice. Given the highly infectious nature of the disease as well as the global scarcity of medical resources; it is only prudent to treat only emergent conditions during the pandemic as we incorporate evidence-based screening and protective measures into our practices. This manuscript is a compilation of evidence-based guidelines for surgical procedures that oculoplastic surgeons can employ during the COVID-19 pandemic. These guidelines also serve as the basic framework upon which further recommendations may be based on in the future, as elective surgeries start being performed on a regular basis.
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- 2020
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16. Commentary: The electromyographic analysis of orbicularis oculi muscle in epiphora
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Mohammad Javed Ali
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Ophthalmology ,RE1-994 - Published
- 2022
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17. Microbiotyping the Sinonasal Microbiome
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Ahmed Bassiouni, Sathish Paramasivan, Arron Shiffer, Matthew R. Dillon, Emily K. Cope, Clare Cooksley, Mahnaz Ramezanpour, Sophia Moraitis, Mohammad Javed Ali, Benjamin S. Bleier, Claudio Callejas, Marjolein E. Cornet, Richard G. Douglas, Daniel Dutra, Christos Georgalas, Richard J. Harvey, Peter H. Hwang, Amber U. Luong, Rodney J. Schlosser, Pongsakorn Tantilipikorn, Marc A. Tewfik, Sarah Vreugde, Peter-John Wormald, J. Gregory Caporaso, and Alkis J. Psaltis
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microbiome ,sinus ,next-generation sequencing ,16S rRNA gene ,chronic rhinosinusitis ,microbiotype ,Microbiology ,QR1-502 - Abstract
This study offers a novel description of the sinonasal microbiome, through an unsupervised machine learning approach combining dimensionality reduction and clustering. We apply our method to the International Sinonasal Microbiome Study (ISMS) dataset of 410 sinus swab samples. We propose three main sinonasal “microbiotypes” or “states”: the first is Corynebacterium-dominated, the second is Staphylococcus-dominated, and the third dominated by the other core genera of the sinonasal microbiome (Streptococcus, Haemophilus, Moraxella, and Pseudomonas). The prevalence of the three microbiotypes studied did not differ between healthy and diseased sinuses, but differences in their distribution were evident based on geography. We also describe a potential reciprocal relationship between Corynebacterium species and Staphylococcus aureus, suggesting that a certain microbial equilibrium between various players is reached in the sinuses. We validate our approach by applying it to a separate 16S rRNA gene sequence dataset of 97 sinus swabs from a different patient cohort. Sinonasal microbiotyping may prove useful in reducing the complexity of describing sinonasal microbiota. It may drive future studies aimed at modeling microbial interactions in the sinuses and in doing so may facilitate the development of a tailored patient-specific approach to the treatment of sinus disease in the future.
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- 2020
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18. A Robust Model System for Retinal Hypoxia: Live Imaging of Calcium Dynamics and Gene Expression Studies in Primary Human Mixed Retinal Culture
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Shahna Shahulhameed, Sarpras Swain, Soumya Jana, Jay Chhablani, Mohammad Javed Ali, Rajeev R. Pappuru, Mudit Tyagi, Sushma Vishwakarma, Nanda Sailaja, Subhabrata Chakrabarti, Lopamudra Giri, and Inderjeet Kaur
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retina ,glia ,calcium spiking ,neurons ,hypoxia ,neurodegeneration ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
The detailed mechanisms underlying oxidative stress that leads to neuroinflammation and neurodegeneration in retinal vascular conditions, including diabetic retinopathy, retinopathy of prematurity etc., remain largely unexplored mainly due to a lack of suitable disease models that can simulate the inherent neuron–glia interactions in human retina. Specifically, establishment of a mixed retinal culture (MRC) containing both neuron and glial cell types remains a challenge due to different conditions required for their optimal growth and differentiation. Here, we establish a novel primary MRC model system containing neurons, astrocytes, Müller glia, and microglia from human donor retina that can be used to study the neuromodulatory effects of glial cells under the stress. The cell characterization based on immunostaining with individual cell type–specific markers and their presence in close vicinity to each other further underscores their utility for studying their cross talk. To the best of our knowledge, this is the first instance of an in vitro model obtained from human donor retina containing four major cell types. Next, we induce hypoxic stress to MRC to investigate if hypoxia activated neuroglia modulates altered gene expression for inflammatory, apoptotic, and angiogenic markers and Ca2+ transients by live cell imaging. Further, we performed k-means clustering of the Ca2+ responses to identify the modification of clustering pattern in stressed condition. Finally, we provide the evidence that the altered Ca2+ transient correlates to differential expression of genes shown to be involved in neuroinflammation, angiogenesis, and neurodegeneration under the hypoxic conditions as seen earlier in human cell lines and animal models of diabetic retinopathy. The major features of the hypoxic conditions in the proposed human MRC model included: increase in microglia activity, chemokine and cytokine expression, and percentage of cells having higher amplitude and frequency of Ca2+ transients. Thus, the proposed experimental system can potentially serve as an ideal in vitro model for studying the neuroinflammatory and neurodegenerative changes in the retina and identifying newer drug targets.
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- 2020
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19. Lysosomal enzymes and mannose 6-phosphate receptors in the lacrimal drainage system: Evidence and its potential implications
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Mohammad Javed Ali, Ashapogu Venugopal, Kavyashree Sakharayapatna Ranganath, and Nadimpalli Siva Kumar
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Enzymes ,etiopathogenesis ,lacrimal drainage ,lysosomes ,mannose 6-phosophate receptors ,primary acquired nasolacrimal duct obstruction ,Ophthalmology ,RE1-994 - Abstract
Purpose: To investigate the presence and patterns of lysosomal enzymes and mannose 6-phosophate receptor (MPRs) in human lacrimal drainage system. Methods: The study was performed on healthy lacrimal sacs and nasolacrimal ducts obtained from exenteration samples immediately after surgery and frozen at −80°C for subsequent analysis. Soluble proteins' extract was used for enzyme assays, sodium dodecyl sulfate–polyacrylamide gel electrophoresis (PAGE), native PAGE, activity staining, and western blot analysis. Membrane proteins were separately assessed for detection of mannose 6-phosphate receptors, MPR 46. Sepharose gels, 4-methylumbelliferyl substrates, and antibodies against common lysosomal enzymes and MPRs were used. Enzyme assays were carried out in triplicate to ascertain the results. Results: Differential lysosomal enzyme activities were documented, and among them acid phosphatase and β-hexosaminidase were found to be high. Western blot analysis using enzyme antibodies and subsequent activity staining confirmed strong signals for moderately expressed enzymes such as fucosidase, glucuronidase, and mannosidase. Membrane extracts demonstrated the presence of MPR 46, which indicates the possible roles of cation-dependent MPRs in lysosomal targeting in human lacrimal drainage system. Conclusion: This study provides a proof of principle for the presence of differential lysosomal activity and mannose 6-phosphate ligand transport receptors in human lacrimal drainage system and hypothesizes the potential implications of their dysfunctions.
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- 2018
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20. Low-cost three-dimensional printed orbital template-assisted patient-specific implants for the correction of spherical orbital implant migration
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Tarjani Vivek Dave, Sweety Tiple, Sandeep Vempati, Mansha Palo, Mohammad Javed Ali, Swathi Kaliki, and Milind N Naik
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Anophthalmic socket ,complication ,implant migration ,orbital implant ,three-dimensional printing ,Ophthalmology ,RE1-994 - Abstract
Purpose: To describe the outcomes of a patient-specific implant (PSI), fabricated using a three-dimensional (3D) printed orbital template and placed in the basin of the inferior orbital fissure to correct inferotemporally migrated spherical orbital implant. Methods: This is a single-center, prospective, consecutive, interventional, case series of six patients, with non-porous, spherical, orbital implant migration that underwent implant recentration surgically with a novel technique. Migration was subclassified either as decentration that did not affect the prosthetic retention or as displacement that affected the prosthetic retention in the eye socket. Only implant displacements were treated. The primary outcome measure was centration of the implant clinically and radiologically, with ability to retain the prosthesis. Results: At a mean follow-up of 21 months, all six orbital spherical implants remained centered. There were no cases of extrusion, exposure, or migration of either implants. There were no cases of PSI displacement. Additional procedures to optimize the aesthetic outcome of the customized ocular prosthesis (COP) required were simultaneous fornix formation suture in three patients, subsequent fornix formation with mucus membrane graft in two patients, and levator resection and sulcus hyaluronic acid gel injection in one patient each. The mean PSI implant weight was 2.66 ± 0.53 g. The mean COP weight was 2.2 ± 0.88 g postoperatively. The median patient satisfaction with the procedure was 9 on 10. Conclusion: A 3D printing-assisted PSI placed in the basin of the inferior orbital fissure allows recentration of the migrated implant over a follow-up of 21 months without complications.
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- 2018
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21. The science and philosophy of manuscript rejection
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Mohammad Javed Ali
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Ophthalmology ,RE1-994 - Published
- 2021
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22. Commentary: Avoiding predatory publishing for early career ophthalmologists
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Mohammad Javed Ali
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Ophthalmology ,RE1-994 - Published
- 2021
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23. Predatory journals and conferences: Analysis of invitation emails from a single clinician-scientist's inbox
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Mohammad Javed Ali
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Ophthalmology ,RE1-994 - Published
- 2021
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24. Periorbital biometric measurements using ImageJ software: Standardisation of technique and assessment of intra- and interobserver variability
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R Rajyalakshmi, Winston D Prakash, Mohammad Javed Ali, and Milind N Naik
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Facial measurements ,ImageJ ,periorbital biometry ,Surgery ,RD1-811 - Abstract
Purpose: To assess the reliability and repeatability of periorbital biometric measurements using ImageJ software and to assess if the horizontal visible iris diameter (HVID) serves as a reliable scale for facial measurements. Methods: This study was a prospective, single-blind, comparative study. Two clinicians performed 12 periorbital measurements on 100 standardised face photographs. Each individual’s HVID was determined by Orbscan IIz and used as a scale for measurements using ImageJ software. All measurements were repeated using the ‘average’ HVID of the study population as a measurement scale. Intraclass correlation coefficient (ICC) and Pearson product-moment coefficient were used as statistical tests to analyse the data. Results: The range of ICC for intra- and interobserver variability was 0.79–0.99 and 0.86–0.99, respectively. Test-retest reliability ranged from 0.66–1.0 to 0.77–0.98, respectively. When average HVID of the study population was used as scale, ICC ranged from 0.83 to 0.99, and the test-retest reliability ranged from 0.83 to 0.96 and the measurements correlated well with recordings done with individual Orbscan HVID measurements. Conclusion: Periorbital biometric measurements using ImageJ software are reproducible and repeatable. Average HVID of the population as measured by Orbscan is a reliable scale for facial measurements.
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- 2017
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25. The SARS-CoV-2, tears, and ocular surface debate: What we know and what we need to know
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Mohammad Javed Ali
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Ophthalmology ,RE1-994 - Published
- 2020
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26. Lacrimal punctal and peripunctal involvement in calcinosis cutis
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Nandini Bothra, Mohammad Javed Ali, Nikolaos Trakos, and Milind N Naik
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Calcinosis cutis ,lacrimal ,punctum ,peripunctal ,Ophthalmology ,RE1-994 - Abstract
Calcinosis cutis is a rare metabolic disorder characterized by cutaneous and subcutaneous deposition of insoluble calcium salts. Ocular adnexal involvement is uncommon but mostly seen in males and in the upper eyelid. Solitary lesions are more common than multiple. The treatment of choice is excision biopsy. Histopathological examination provides a definitive diagnosis. Directed systemic investigations help to rule out underlying pathologies and aids further management. The present case reports the punctal and peripunctal involvement in a case of ocular adnexal calcinosis cutis.
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- 2018
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27. Assessing the outcomes of mini-Monoka stent dilatation for primary punctal stenosis using the lacrimal symptom questionnaire
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Shweta Gupta, Mohammad Javed Ali, Mohammad Hasnat Ali, and Milind N Naik
- Subjects
Lac-Q ,lacrimal ,Monoka stents ,punctal stenosis ,quality of life ,questionnaire ,Ophthalmology ,RE1-994 - Abstract
Purpose: The objective of this study was to assess the quality of outcomes of mini-Monoka stent dilatation for punctal stenosis using the lacrimal symptom (Lac-Q) questionnaire. Methods: Prospective interventional case series of 45 eyes of 25 consecutive patients who underwent mini-Monoka stent dilatation for primary punctal stenosis were included in the study. The stents were extubated at 4 weeks. The Lac-Q questionnaire was administered preoperatively and at 12 weeks and 24 weeks following the intervention. Outcomes assessed were anatomical success, functional success, and changes in the questionnaire scores including total, social impact, and lacrimal symptom scores. Statistical analysis was performed using the Wilcoxon signed-rank test, and the probability of obtaining a certain score was calculated using the multinomial log-linear model. P < 0.05 was considered as significant. Results: Monoka stents were performed for 45 eyes. A total of 75 questionnaire responses were analyzed. At the last follow-up of 24 weeks, the anatomical and functional success rates were 93.3% (42/45). The changes in the mean total score (7.68 preoperatively to 0.82 at 24-week follow-up) were statistically significant (P ≤ 0.001). The mean social impact scores showed significant improvement postoperatively from 4.06 to 0.53 (P ≤ 0.001). The mean lacrimal symptom score changed from a preoperative value of 3.62 to 0.28 (P ≤ 0.001) at the last follow-up. Postoperative scoring correlated well with the anatomical and functional success rates. The symptom scores corroborated with changes in the clinical outcomes. Conclusion: Mini-Monoka stent dilatation is a very effective intervention for the management of primary punctal stenosis, and Lac-Q questionnaire is a simple and useful tool to evaluate the quality of outcomes in such cases.
- Published
- 2018
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28. Commentary: Propensity of aerosol and droplet creation during oculoplastic procedures: A risk assessment with high-speed imaging amidst COVID-19 pandemic
- Author
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Mohammad Javed Ali
- Subjects
Ophthalmology ,RE1-994 - Published
- 2021
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29. Impact factor under attack! Are the criticisms justified?
- Author
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Mohammad Javed Ali
- Subjects
Ophthalmology ,RE1-994 - Published
- 2021
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30. Commentary: Subjective outcomes and quality of life following external dacryocystorhinostomy
- Author
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Mohammad Javed Ali
- Subjects
Ophthalmology ,RE1-994 - Published
- 2021
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- View/download PDF
31. Preferences of ophthalmic plastics patients and their caregivers toward the doctors′ attire and initial communications: A tertiary eye care study
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Sadiya Iram, Winston D Prakash, Mohammad Javed Ali, Tarjani Vivek Dave, and Milind N Naik
- Subjects
Communication ,doctor′s attire ,doctor′s dress ,patient′s expectations ,physician-patient relationship ,Ophthalmology ,RE1-994 - Abstract
Purpose: The aims of this study were to determine the acceptability levels of different styles of the doctors′ dress and the expectations from the initial phases of physician-patient encounter. Methods: The study design was a cross-sectional descriptive type using the survey methodology. A survey based on a five-point questionnaire was performed on all consecutive patients or their caregivers, aged ≥15, visiting the ophthalmic plastics outpatient clinics at a tertiary eye care institute. The participants were shown three sets of photographs and were required to answer a questionnaire which consisted of five questions. Data collected include participant demographics and their preferences with regards to the physician′s attire and initial communications. Results: A total of 300 consecutive responses were analyzed. The mean age of the participants was 37.2 years. Among the participants, 87.6% (263/300) and 90.3% (271/300) preferred a white coat for the male and female physicians, respectively (P < 0.001). The most common second preference was scrubs for both the males and female physicians. 92.3% (277/300) preferred the attire to have an identification display. The overwhelming majority of respondents (95.6%, 287/300) preferred the physicians to address them by their name and 98.6% (296/300) liked if their physicians smiled while addressing them. Conclusions: White coat was the main preferred attire among respondents. Increased awareness of the patient′s expectations plays a crucial role in enhancing their satisfaction.
- Published
- 2016
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32. Outcomes of endoscopic dacryocystorhinostomy: Experience of a fellowship trainee at a tertiary care center
- Author
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Saurabh Kamal, Mohammad Javed Ali, and Akshay Gopinathan Nair
- Subjects
Endoscopic dacryocystorhinostomy ,lacrimal duct ,nasolacrimal duct obstruction ,oculoplasty fellowship ,trainee ,Ophthalmology ,RE1-994 - Abstract
Aim: The study aims to report a single trainee's experience of learning and performing endoscopic endonasal dacryocystorhinostomy (En-DCR). Settings and Design: This study was a retrospective, interventional case series. Subjects and Methods: Fifty-four eyes of fifty patients presenting at a tertiary eye care center over 1 year were included in the study. All cases underwent endoscopic DCR with mitomycin-C and silicone intubation. The parameters studied included demographics, clinical features, intraoperative details, and postoperative ostium evaluation. Stent removal and nasal endoscopy were performed at 6 weeks and a further ostium evaluation at 3 and 6 months following surgery. Anatomical success rate was defined as patent irrigation, and functional success rate was defined as positive functional endoscopic dye test and absence of epiphora. Results: Fifty-four eyes of fifty patients were operated, and three cases were lost to follow-up after surgery. The mean age at presentation was 34 (4–75) years. Clinical diagnosis included primary acquired nasolacrimal duct (NLD) obstruction in 72% (39/54), acute dacryocystitis in 15% (8/54), failed DCR in 7% (4/54), and persistent congenital NLD obstruction in 5% (3/54). The first five cases needed intervention by the mentor for superior osteotomy. Common variations in anatomical landmarks were posterior location of sac, large ethmoidal bulla, high internal common opening, and thick maxillary bone. Surgical time taken in the last 27 eyes was significantly lesser compared to the surgical duration taken in the initial 27 cases (P < 0.05). Anatomical and functional success rate was 94% (48/51) at 6 months follow-up period. Conclusions: Endoscopic En-DCR has a good success rate when performed by oculoplastic surgery trainees. Nasal anatomical variations, instrument handling, and adaptation to monocular view of endoscope are few of the challenges for beginners. Structured skill transfer can help trainees to learn and perform En-DCR with acceptable success rates.
- Published
- 2016
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33. Mitomycin-C in dacryocystorhinostomy: From experimentation to implementation and the road ahead: A review
- Author
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Akshay Gopinathan Nair and Mohammad Javed Ali
- Subjects
Advanced glaucoma ,optical coherence tomography ,reproducibility ,retinal nerve fiber layer thickness ,spectral domain ,Ophthalmology ,residents ,surgical training ,survey ,Cytokine ,diabetic macular edema ,Macular GHPC ,pulse cyclophosphamide ,serpiginous choroidopathy ,Contact lens ,Corvis ST ,intraocular pressure ,Confocal microscopy ,meibomian gland ,meibomian gland microenvironment ,papillary formation ,vernal keratoconjunctivitis ,Eye ,intra-arterial chemotherapy ,retinoblastoma ,tumor ,Dacryocystorhinostomy ,intubation ,lacrimal surgery ,mitomycin-C ,nasolacrimal duct obstruction ,primary acquired nasolacrimal duct obstruction ,RE1-994 - Abstract
Dacryocystorhinostomy (DCR) is the procedure of choice in patients with epiphora due to primary acquired nasolacrimal duct obstruction. The evolution of surgical tools, fiber-optic endoscopes, effective anesthesia techniques, and the adjunct use of antimetabolites intraoperatively; namely mitomycin-C (MMC) have significantly contributed to the advancement of DCR surgery. MMC is a systemic chemotherapeutic agent derived from Streptomyces caespitosus that inhibits the synthesis of DNA, cellular RNA, and protein by inhibiting the synthesis of collagen by fibroblasts. Even the cellular changes in the human nasal mucosal fibroblasts induced by MMC at an ultrastructural level have been documented. There, however, seems to be a lack of consensus regarding MMC: The dosage, the route of delivery/application, the time of exposure and subsequently what role each of these variables plays in the final outcome of the surgery. In this review, an attempt is made to objectively examine all the evidence regarding the role of MMC in DCR. MMC appears to improve the success rate of DCR.
- Published
- 2015
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34. External dacryocystorhinostomy: Tips and tricks
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Mohammad Javed Ali, Milind N Naik, and Santosh G Honavar
- Subjects
Congenital nasolacrimal duct obstruction ,dacryocystorhinostomy ,primary acquired nasolacrimal duct obstruction ,secondary acquired lacrimal duct obstruction ,Ophthalmology ,RE1-994 - Abstract
Dacryocystorhinostomy or DCR is one of the most common oculoplastics surgery performed. It is a bypass procedure that creates an anastomosis between the lacrimal sac and the nasal mucosa via a bony ostium. It may be performed through an external skin incision or intranasally with or without endoscopic visualization. This article will discuss the indications, goals, and simple techniques for a successful outcome of an external DCR.
- Published
- 2012
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35. Pediatric dacryocystorhinostomy
- Author
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Mohammad Javed Ali
- Subjects
Ophthalmology ,RE1-994 - Published
- 2017
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36. Cardiobacterium hominis-induced acute dacryocystitis and lacrimal abscess
- Author
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Guru Prasad Manderwad, Manjulatha Kodiganti, and Mohammad Javed Ali
- Subjects
Mean deviation ,non-proliferative diabetic retinopathy ,short fluctuations ,short wave automated perimetry ,standard automated perimetry ,Antimicrobial effect ,conventional silicone oil ,endophthalmitis agents ,heavy silicone oil ,Keratoconus ,modified deep anterior lamellar keratoplasty ,steep corneal curvature ,Bevacizumab ,branch retinal vein occlusion ,intravitreal ,macular edema ,triamcinolone ,Blindness ,disability evaluation ,quality of life ,visual acuity ,Mitomycin C ,pterygium recurrence ,pterygium surgery ,subconjunctival bevacizumab injection ,Imaging in glaucoma ,optical coherence tomography ,pediatric ,retinal nerve fiber layer ,Autogenous tissue grafting ,lacrimal drainage system ,lacrimal fossa ,bypass surgery ,Cornea ,excimer laser ,growth factors ,myopia ,photorefractive keratectomy ,Triamcinolone acetonide ,human trabecular meshwork cells ,in vitro ,Hyperopic implantable collamer lens ,myopic implantable collamer lens ,optical quality ,wavefront aberrations ,Dacryocystorhinostomy ,nasolacrimal duct obstruction ,silicone tube ,Age ,anterior chamber depth ,body height ,primary angle closure glaucoma ,sex ,Children ,eye injury vitrectomy study ,eye injury ,trauma ,vitrectomy ,Corneal biomechanics ,corneal hysteresis ,corneal resistance factor ,intraocular pressure ,keratoplasty ,ocular response analyser ,Aspherical intraocular lens ,quality of vision ,spherical aberrations ,Benign ,excision ,lids and caruncle ,no recurrence ,ocular FH ,inflammation ,sterile endophthalmitis ,Carotid artery disease ,ocular ischemic syndrome ,retinal emboli ,cataract ,India ,low vision ,population ,Awareness ,glaucoma ,knowledge ,North India ,Aphakia ,capsular support ,inadequate ,intraocular lens implantation ,iris fixation ,Extra macular BRVO ,hyperhomocysteinemia ,multiple BRVO ,Medial canthus ,recurrent ,retiform hemangioendothelioma ,Optic atrophy ,osteopetrosis ,Lacrimal abscess ,dacryocystitis ,cardiobacterium hominis ,Ophthalmology ,RE1-994 - Abstract
Cardiobacterium hominis is a member of the HACEK (Haemophilus sp., Actinobacillus actinomycetemcomitans, C. hominis, Eikenella corrodens, and Kingella kingae) group commonly associated with endocarditits and is normally present in the respiratory tract. We describe the first case of acute dacryocystitis with lacrimal abscess caused by C. hominis along with a brief review of the literature. The patient responded to oral and topical ciprofloxacin after incision and drainage and awaits dacryocystorhinostomy.
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- 2014
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37. Distant metastatic retinoblastoma without central nervous system involvement
- Author
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Mohammad Javed Ali, Santosh G Honavar, and Vijay Anand Reddy
- Subjects
Anxiety ,cataract ,intraocular pressure ,melatonin ,pain ,topical anesthesia ,Binocular vision ,near point of convergence ,visual symptoms ,Electron microscopy ,intravitreal injection ,light microscopy ,methotrexate ,rabbit ,retina ,Contact lens ,healthcare workers ,health professionals ,Collagen implant ,mitomycin C ,ologen ,trabeculectomy ,Air ,cataract surgery ,inflammation ,Carotid-cavernous fistula ,endovascular treatment ,Graves′ ophthalmopathy ,Macular edema ,optical coherence tomography ,pituitary adenoma ,Choroidal neovascular membrane ,idiopathic macular telangiectasia ,photodynamic therapy ,ranibizumab ,Ahmed Glaucoma Valve ,conjunctival erosion ,Doxycycline ,Autologous stem cell rescue ,bone marrow ,metastasis ,retinoblastoma ,Ophthalmology ,RE1-994 - Abstract
Retinoblastoma is the most common intraocular malignancy in children, with a reported incidence ranging from 1 in 15,000 to 1 in 18,000 live births. Metastatic retinoblastoma is rare in developed countries, with a reported range from 4.8% in the United States to 5.8% in the United Kingdom. However, the frequency reported from developing countries varies from 9 to 11% at presentation. The mortality is very high owing to late presentations, delayed diagnosis compounded by socio-economic factors. The management of metastatic retinoblastoma is evolving, but it is still a challenge in pediatric oncology. We present a case of an extensive skeletal metastasis that initially presented as a massive orbital retinoblastoma.
- Published
- 2013
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38. Orbital solitary fibrous tumor: A rare clinicopathologic correlation and review of literature
- Author
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Mohammad Javed Ali, Santosh G Honavar, Milind N Naik, and Geeta K Vemuganti
- Subjects
BCL-2 ,CD34 ,hemangiopericytoma ,solitary fibrous tumor ,orbit ,Medicine - Abstract
Orbital solitary fibrous tumor (SFT) is a rare tumor and orbits are a very uncommon site. It is mostly noted to arise from mesenchymal structures like pleura and peritoneum. The diagnosis of orbital SFT cannot be made with certainty on clinical or radiological evaluation alone and requires immunohistochemical studies for confirmation. Orbital SFT′s usually show an indolent clinical course and a complete cure can usually be achieved with complete resection. We describe clinical presentations, radiological and operative findings, and pathological features of a patient with orbital SFT along with a brief review of literature.
- Published
- 2013
39. Comparison of immersion ultrasonography, ultrasound biomicroscopy and anterior segment optical coherence tomography in the evaluation of traumatic phacoceles
- Author
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Harikrishna Vodapalli, Somasheila I Murthy, Subhadra Jalali, Mohammad Javed Ali, and Padmaja Kumari Rani
- Subjects
Band-shaped keratopathy ,corneal dystrophy ,phototherapeutic keratectomy ,phototherapeutic keratectomy with amniotic membrane grafts ,scar ,and Salzmann nodular degeneration ,Kaplan Meier survival analysis ,multivariate Cox Proportional Hazard Regression analysis ,optical penetrating keratoplasties ,Myopia ,phakic refractive intraocular lens ,refractive surgery ,23-Gauge vitrectomy ,rhegmatogenous retinal detachment ,sutureless vitrectomy ,Deep anterior lamellar keratoplasty ,endothelial cell density ,keratoconus ,penetrating keratoplasty ,Anterior chamber contamination ,cataract surgery ,conjunctival flora ,diabetics ,Cardiovascular disease ,diabetes ,diabetic retinopathy ,framingham risk score ,Central macular thickness ,multifocal electroretinogram ,normative database ,optical coherence tomography ,Macular microhole ,microperimetry ,spectral domain optical coherence tomography ,Age-related macular degeneration ,choroidal neovascularization ,photodynamic therapy ,vitreous traction ,Descemet membrane endothelial keratoplasty with a stromal rim ,endothelial keratoplasty ,posterior lamellar keratoplasty ,posterior polymorphous corneal dystrophy ,Indocyanine green angiogram ,intravitreal bevacizumab ,laser photocoagulation ,peripheral hemorrhagic exudative chorioretinopathy ,polypoidal choroidal vasculopathy ,Anterior segment optical coherence tomography ,B-scan ultrasonography ,phacocele ,ultrasound biomicroscopy ,Ophthalmology ,RE1-994 - Abstract
Blunt ocular trauma in the elderly can result in anterior dislocation of the crystalline lens into the subconjunctival space (phacocele). Although rare, this presentation can be missed, especially if the patient presents several days after the injury and if the lid is not everted on examination. While a careful clinical examination is adequate in the diagnosis, imaging techniques can be put to use for the accurate location of the associated sclera rupture. We report three cases of post-traumatic phacocele wherein ultrasound biomicroscopy (UBM) was compared to the anterior segment optical coherence tomography (AS-OCT) and B-scan ultrasonography (B-scan), in order to establish the best imaging tool for this condition. We concluded, based on image quality, that UBM could be the imaging modality of choice to aid in the diagnosis of phacocele.
- Published
- 2012
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40. Orbital solitary fibrous tumor: A clinicopathologic correlation and review of literature
- Author
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Mohammad Javed Ali, Santosh G Honavar, Milind N Naik, and Geeta K Vemuganti
- Subjects
BCL-2 ,CD-34 ,hemangiopericytoma ,orbit ,proptosis ,solitary fibrous tumor ,Ophthalmology ,RE1-994 - Abstract
Orbital solitary fibrous tumor (SFT) is a rare tumor originating from the mesenchyme. Initially described in the pleura and subsequently in other mesenchymal structures, orbit continues to be one of the uncommon extrapleural sites. The diagnosis of orbital SFT cannot be made with certainty on clinical or radiological evaluation and requires histologic studies with immunohistochemical confirmation for which CD 34 is the most specific diagnostic test. We describe clinical presentations, radiological and operative findings, and pathological features of a patient with orbital SFT along with a review of literature.
- Published
- 2011
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41. Rhinosporidiosis of the tarsal conjunctiva
- Author
-
Akshay Gopinathan Nair, Mohammad Javed Ali, Swathi Kaliki, and Milind N Naik
- Subjects
Community ,digital imaging ,Karnataka Internet-assisted Diagnosis of Retinopathy of Prematurity ,retinopathy of prematurity ,telemedicine ,universal screening ,Age-related macular degeneration ,en-face optical coherence tomography ,polypoidal choroidal vasculopathy ,Choroid ,enhanced depth imaging technique ,swept source optical coherence tomography ,Choroidal imaging ,choroidal thickness ,retinal dystrophies ,Choroidal neovascularization ,idiopathic juxtafoveal telangiectasis ,juxtafoveal retinal telangiectasia ,lutein ,macular edema ,macular pigment ,macular telangiectasia ,Müller cells ,parafoveal telangiectasis ,perifoveal telangiectasis ,retinal angiomatous proliferation ,retinal telangiectasis ,subretinal neovascularization ,zeaxanthin ,Microscope-integrated optical coherence tomography ,RESCAN ,spectral domain optical coherence tomography ,vitreoretinal surgery ,Central reading center ,randomized controlled trial ,standard operating procedure ,Fundus autofluorescence ,lipofuscin ,retina ,retinal imaging ,Cystoid macular edema ,dexamethasone implant ,external limiting membrane ,optical coherence tomography ,Ozurdex ,serous retinal detachment ,uveitis ,Adaptive optics ,axial length ,cone density ,myopia ,Artifact ,foveal diameter ,foveal slope ,ocular magnification ,Emmetropization ,hand-held ,spectral-domain optical coherence tomography ,visual acuity ,Accommodating intraocular lenses ,Crystalens HD ,effective lens position ,intraocular lenses power ,keratometry ,Choroidal thickness ,dynamic exercise ,exercise ,Breast carcinoma ,eye ,metastasis ,optic disk ,optic nerve ,tumor ,Choroidal osteoma ,enhanced depth imaging ,morphology ,Fundus autofluorescence imaging ,retinal phototoxicity ,solar radiation ,Biopsy ,conjunctiva ,lung ,mucoepidermoid carcinoma ,Advanced glaucoma ,ExPress shunt ,trabeculectomy and ExPress shunt ,Conjunctiva ,conjunctivosporidiosis ,rhinosporidiosis ,tarsus ,Ophthalmology ,RE1-994 - Abstract
Rhinosporidiosis is a rare infection caused by Rhinosporidium seeberi, an organism classified in its own class, mesomycetozoea. It commonly affects mucus membranes namely the nasal mucosa, pharynx and the conjunctiva. We present the case of an 8-year-old female who presented with a flat, red, vascular, fleshy, pedunculated mass arising from the tarsal conjunctiva of the right upper eyelid. The mass was completely excised. On histopathological examination, multiple sporangia were seen in various stages of degeneration, consistent with rhinosporidiosis. The diagnosis of rhinosporidiosis is based solely on its microscopic features, and the treatment is surgical excision. This condition is endemic in the temperate regions of the Indian subcontinent, but it has been known to occur even in the colder regions of North America and Eastern Europe. Although a rare clinical entity, the possibility of rhinosporidiosis must be borne in mind when evaluating any polypoidal conjunctival mass.
- Published
- 2015
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42. Establishing human lacrimal gland cultures with secretory function.
- Author
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Shubha Tiwari, Mohammad Javed Ali, Murali M S Balla, Milind N Naik, Santosh G Honavar, Vijay Anand P Reddy, and Geeta K Vemuganti
- Subjects
Medicine ,Science - Abstract
PURPOSE: Dry eye syndrome is a multifactorial chronic disabling disease mainly caused by the functional disruptions in the lacrimal gland. The treatment involves palliation like ocular surface lubrication and rehydration. Cell therapy involving replacement of the gland is a promising alternative for providing long-term relief to patients. This study aimed to establish functionally competent lacrimal gland cultures in-vitro and explore the presence of stem cells in the native gland and the established in-vitro cultures. METHODS: Fresh human lacrimal gland from patients undergoing exenteration was harvested for cultures after IRB approval. The freshly isolated cells were evaluated by flow cytometry for expression of stem cell markers ABCG2, high ALDH1 levels and c-kit. Cultures were established on Matrigel, collagen and HAM and the cultured cells evaluated for the presence of stem cell markers and differentiating markers of epithelial (E-cadherin, EpCAM), mesenchymal (Vimentin, CD90) and myofibroblastic (α-SMA, S-100) origin by flow cytometry and immunocytochemistry. The conditioned media was tested for secretory proteins (scIgA, lactoferrin, lysozyme) post carbachol (100 µM) stimulation by ELISA. RESULTS: Native human lacrimal gland expressed ABCG2 (mean±SEM: 3.1±0.61%), high ALDH1 (3.8±1.26%) and c-kit (6.7±2.0%). Lacrimal gland cultures formed a monolayer, in order of preference on Matrigel, collagen and HAM within 15-20 days, containing a heterogeneous population of stem-like and differentiated cells. The epithelial cells formed 'spherules' with duct like connections, suggestive of ductal origin. The levels of scIgA (47.43 to 61.56 ng/ml), lysozyme (24.36 to 144.74 ng/ml) and lactoferrin (32.45 to 40.31 ng/ml) in the conditioned media were significantly higher than the negative controls (p
- Published
- 2012
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43. Retinal astrocytic hamartoma and Bourneville′s disease
- Author
-
Mohammad Javed Ali, Santosh G Honavar, and Milind N Naik
- Subjects
Ophthalmology ,RE1-994 - Published
- 2012
- Full Text
- View/download PDF
44. Optic nerve infiltration in relapse of acute lymphoblastic leukemia
- Author
-
Mohammad Javed Ali and Santosh G Honavar
- Subjects
Ophthalmology ,RE1-994 - Published
- 2011
- Full Text
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45. Long-term outcomes of revision endoscopic dacryocystorhinostomy aided by 4-mm coronary balloon catheter dacryoplasty
- Author
-
Nandini Bothra and Mohammad Javed Ali
- Subjects
medicine.medical_specialty ,Catheters ,medicine.medical_treatment ,Dacryocystorhinostomy ,India ,failed DCR ,Balloon ,Endoscopic dacryocystorhinostomy ,lcsh:Ophthalmology ,medicine ,Long term outcomes ,Humans ,Surgical Technique ,Retrospective Studies ,business.industry ,Balloon catheter ,lacrimal ,Endoscopy ,computer.file_format ,Middle Aged ,Lacrimal sac ,Surgery ,Ophthalmology ,Ostium ,medicine.anatomical_structure ,Treatment Outcome ,lcsh:RE1-994 ,endoscopic DCR ,ICO ,Balloon dacryoplasty ,business ,computer ,Nasolacrimal Duct - Abstract
Our purpose was assess the long-term efficacy of 4-mm coronary balloon catheter dacryoplasty in revision endoscopic dacryocystorhinostomy (RevEnDCR). This retrospective interventional case-series was performed for patients who underwent RevEnDCR aided by a 4-mm coronary balloon catheter (CBC) dacryoplasty. The indications for the surgery were previously failed DCRs by external or endoscopic approach where the ostium showed near total cicatrization with or without the presence of organized granuloma threatening the internal common opening (ICO). The coronary balloon (4 × 10 mm, SPALNO, Cardiomac, Haryana, India) with the guidewire was used and a minimum of >12 months of follow-up was considered for analysis. Ten lacrimal systems of eight patients with mean age of 48.8 years underwent CBC-assisted revision endoscopic DCR. Of the 10 failed DCRs, 6 had a previous external approach DCR and 4 were endoscopic DCRs. Grossly stenosed ostium with near total cicatricial closure were noted in half of the patients (50%, 5/10) while the remaining half, in addition, showed organized granulomas threatening the ICO. The surgical technique using CBC was found to be minimally invasive, easy to perform with multiple advantages like uniform clearance of the area in front of ICO and more predictable lacrimal sac flaps. At a mean follow-up of 20 months, anatomical and functional success were achieved in 90% (9/10) of the eyes. We conclude that coronary balloon catheter-assisted revision endoscopic DCR is a minimally invasive and viable alternative in select group of patients of failed DCR with near total cicatrisation or organized granulomas threatening ICO.
- Published
- 2021
46. Severe ocular and adnexal complications in dengue hemorrhagic fever: A report of 29 eyes
- Author
-
Rajeev R Pappuru, Mohammad Javed Ali, Raja Narayanan, Somasheila I Murthy, Tarjani Vivek Dave, VS Vijitha, and Vivek Pravin Dave
- Subjects
Adult ,medicine.medical_specialty ,Visual acuity ,Blood transfusion ,Adolescent ,genetic structures ,medicine.medical_treatment ,scleral melt ,Vitrectomy ,Dengue fever ,necrosis ,Young Adult ,Endophthalmitis ,lcsh:Ophthalmology ,panophthalmitis ,medicine ,Humans ,Special Focus on Ocular Inflammation, Original Article ,Panophthalmitis ,Severe Dengue ,Retrospective Studies ,business.industry ,Middle Aged ,medicine.disease ,dengue ,eye diseases ,Surgery ,Ophthalmology ,phthisis bulbi ,lcsh:RE1-994 ,sense organs ,Phthisis bulbi ,medicine.symptom ,Orbital cellulitis ,business - Abstract
Purpose: To describe a series of sight-threatening ocular and adnexal involvement associated with dengue hemorrhagic fever and their treatment options. Methods: Retrospective, interventional, non-comparative case series. Medical records of patients who presented with ocular symptoms attributed to dengue hemorrhagic fever were reviewed. Demography, presenting features, and treatment outcomes were recorded. Outcome measures assessed included globe salvage, vision salvage, and visual improvement. The outcome was deemed as favorable if vision salvage was possible and unfavorable if the vision was lost. Results: Twenty-nine eyes of 23 patients were included. Bilateral ocular involvement was seen in 6 (26%) patients. The mean age was 37.62 ± 18.68 years (range: 14 to 81 years). Coexistent diabetes mellitus and enteric fever were present in three patients. History of blood transfusion was present in nine (40%) and thrombocytopenia in eight (35%) patients at the time of presentation with ocular complaints. Presenting features included endophthalmitis in 11 (38%), panophthalmitis in 10 (35%), orbital cellulitis with panophthalmitis in four (14%), isolated corneal or scleral melt in three (10%), and orbital hemorrhage with panophthalmitis in one (3%) patient. Globe salvage was achieved in 21/29 eyes (72.4%), vision salvage in 6/29 eyes (20.68%), and improvement in visual acuity was noted in 5/29 eyes (17.24%). Logistic regression analysis revealed no significant effect of any clinical-microbiological factors on globe salvage, vision salvage, and visual improvement. However, visual improvement and globe salvage were possible in eyes that underwent early endoscopic vitrectomy. Conclusion: Dengue fever can present with sight-threatening ocular and adnexal inflammation resulting in endophthalmitis and panophthalmitis, orbital cellulitis, corneal and scleral melt, and orbital hemorrhage. Early vitrectomy may improve vision and globe salvage in cases with significant vitritis.
- Published
- 2021
47. All India Ophthalmological Society - Oculoplastics Association of India consensus statement on preferred practices in oculoplasty and lacrimal surgery during the COVID-19 pandemic
- Author
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Apjit Kaur Chhabra, SM Betharia, Mandeep S Bajaj, Bipasha Mukherjee, Mukesh Sharma, Raghuraj Hegde, Anita Sethi, Ashok K. Grover, Jayanta Kr Das, Mohammad Javed Ali, Kasturi Bhattacharjee, Gagan Dudeja, Usha Singh, Akshay Gopinathan Nair, Lakshmi Mahesh, Santosh G Honavar, and Usha Kim
- Subjects
botox ,2019-20 coronavirus outbreak ,orbital surgery ,Consensus ,Coronavirus disease 2019 (COVID-19) ,media_common.quotation_subject ,Pneumonia, Viral ,coronavirus ,India ,eyelid surgery ,Disease ,Risk Assessment ,Lacrimal surgery ,dcr ,Scarcity ,lockdown ,03 medical and health sciences ,Betacoronavirus ,0302 clinical medicine ,lcsh:Ophthalmology ,Pandemic ,ptosis ,Medicine ,Humans ,Oculoplastics ,guidelines ,Practice Patterns, Physicians' ,Surgery, Plastic ,Pandemics ,Societies, Medical ,media_common ,Lacrimal Apparatus Diseases ,business.industry ,SARS-CoV-2 ,precautions ,blepharoplasty ,COVID-19 ,Surgical procedures ,medicine.disease ,ophthalmology ,lcsh:RE1-994 ,030221 ophthalmology & optometry ,Medical emergency ,business ,Coronavirus Infections ,corona ,030217 neurology & neurosurgery ,dacryocystorhinostomy - Abstract
Oculoplastic surgeries encompass both emergency surgeries for traumatic conditions and infectious disorders as well as elective aesthetic procedures. The COVID-19 pandemic has brought about a drastic change in this practice. Given the highly infectious nature of the disease as well as the global scarcity of medical resources; it is only prudent to treat only emergent conditions during the pandemic as we incorporate evidence-based screening and protective measures into our practices. This manuscript is a compilation of evidence-based guidelines for surgical procedures that oculoplastic surgeons can employ during the COVID-19 pandemic. These guidelines also serve as the basic framework upon which further recommendations may be based on in the future, as elective surgeries start being performed on a regular basis.
- Published
- 2020
48. Comparative study of stenting and ostium packing in Endoscopic Dacryocystorhinostomy for Primary Acquired Nasolacrimal Duct Obstruction
- Author
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Joyce K.Y. Chin, Luke Yeung, Chi-Lai Li, Kelvin K.L. Chong, Antony Law, Vincent Lam, Mohammad Javed Ali, Alvin L. Young, Regine Y.C. Chan, and Hunter K.L. Yuen
- Subjects
Male ,medicine.medical_specialty ,medicine.medical_treatment ,Lacrimal apparatus diseases ,lcsh:Medicine ,Osteotomy ,Endoscopic dacryocystorhinostomy ,Article ,03 medical and health sciences ,0302 clinical medicine ,Lacrimal Duct Obstruction ,Medicine ,Intubation ,Humans ,030223 otorhinolaryngology ,lcsh:Science ,Aged ,Retrospective Studies ,Absorbable gelatin sponge ,Multidisciplinary ,Adult patients ,business.industry ,Primary acquired nasolacrimal duct obstruction ,lcsh:R ,Retrospective cohort study ,Endoscopy ,Middle Aged ,Bandages ,Surgery ,Ostium ,Treatment Outcome ,030221 ophthalmology & optometry ,Female ,Stents ,lcsh:Q ,business ,Dacryocystorhinostomy ,Nasolacrimal Duct ,Follow-Up Studies - Abstract
In this retrospective study, we compared the efficacy and safety of mechanical adjuvants in mucosal-sparing, mechanical endoscopic dacryocystorhinostomy (MMED) for primary acquired nasolacrimal duct obstruction (PANDO). 116 adult patients (90 female) aged 61 ± 11 received one of the following after MMED without topical mitomycin: no stenting or packing (group 1, n = 25), 1-week ostium packing by ribbon gauze (group 2, n = 29) or non-medicated absorbable gelatin sponge (group 3, n = 25), 8-week bicanalicular stenting (group 4, n = 28). 104 patients(92%) provided 12-month outcomes. Number of patients, age, gender, surgeon, and osteotomy size were comparable among groups (p = 0.4–0.9). Marginal significance was found in anatomical (group 1:80%, group 2:96.6%, group 3:96%, group 4:96.4%, p = 0.05) but not functional success (group 1:85%, group 2:85.7%, group 3:83.3%, group 4:88.9%, p = 0.75) at postoperative 12-month. Patients receiving any packing or stenting achieved better anatomical (96% versus 80%, p = 0.015) but not functional success (85% versus 86%, p = 0.90) compared to those receiving none. More patients receiving stenting developed postoperative granuloma than those who did not (87% versus 63%, p = 0.04). 1-week ostium packing was found to be as effective as 8-week bicanalicular intubation in improving anatomical outcome after MMED for PANDO. Functional outcome, however, did not differ among patients receiving mechanical adjuvant or not.
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- 2020
49. Microbiotyping the Sinonasal Microbiome
- Author
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Rodney J. Schlosser, J. Gregory Caporaso, Richard G. Douglas, Clare Cooksley, Emily K. Cope, Sophia Moraitis, Amber U Luong, Mohammad Javed Ali, Claudio Callejas, Marc A. Tewfik, Christos Georgalas, Arron Shiffer, Marjolein E. Cornet, Matthew R. Dillon, Peter-John Wormald, Richard J. Harvey, Mahnaz Ramezanpour, Sarah Vreugde, Alkis J. Psaltis, Peter H. Hwang, Sathish Paramasivan, Pongsakorn Tantilipikorn, Daniel Dutra, Ahmed Bassiouni, and Benjamin S. Bleier
- Subjects
0301 basic medicine ,Microbiology (medical) ,Future studies ,Chronic rhinosinusitis ,Staphylococcus ,030106 microbiology ,Immunology ,lcsh:QR1-502 ,microbiome ,Computational biology ,Corynebacterium species ,Microbiology ,lcsh:Microbiology ,03 medical and health sciences ,Cellular and Infection Microbiology ,paranasal sinuses ,RNA, Ribosomal, 16S ,Sinus disease ,medicine ,Humans ,Microbiome ,Sinusitis ,Sinus (anatomy) ,Original Research ,Microbiota ,chronic rhinosinusitis ,microbiotype ,030104 developmental biology ,Infectious Diseases ,medicine.anatomical_structure ,Paranasal sinuses ,Chronic Disease ,sinus ,next-generation sequencing ,16S rRNA gene ,Gene sequence - Abstract
This study offers a novel description of the sinonasal microbiome, through an unsupervised machine learning approach combining dimensionality reduction and clustering. We apply our method to the International Sinonasal Microbiome Study (ISMS) dataset of 410 sinus swab samples. We propose three main sinonasal “microbiotypes” or “states”: the first is Corynebacterium-dominated, the second is Staphylococcus-dominated, and the third dominated by the other core genera of the sinonasal microbiome (Streptococcus, Haemophilus, Moraxella, and Pseudomonas). The prevalence of the three microbiotypes studied did not differ between healthy and diseased sinuses, but differences in their distribution were evident based on geography. We also describe a potential reciprocal relationship between Corynebacterium species and Staphylococcus aureus, suggesting that a certain microbial equilibrium between various players is reached in the sinuses. We validate our approach by applying it to a separate 16S rRNA gene sequence dataset of 97 sinus swabs from a different patient cohort. Sinonasal microbiotyping may prove useful in reducing the complexity of describing sinonasal microbiota. It may drive future studies aimed at modeling microbial interactions in the sinuses and in doing so may facilitate the development of a tailored patient-specific approach to the treatment of sinus disease in the future.
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- 2020
50. A Robust Model System for Retinal Hypoxia: Live Imaging of Calcium Dynamics and Gene Expression Studies in Primary Human Mixed Retinal Culture
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Subhabrata Chakrabarti, Inderjeet Kaur, Lopamudra Giri, Sushma Vishwakarma, Mohammad Javed Ali, Jay Chhablani, Rajeev R Pappuru, Sarpras Swain, Nanda Sailaja, Shahna Shahulhameed, Soumya Jana, and Mudit Tyagi
- Subjects
0301 basic medicine ,Cell type ,retina ,glia ,neurons ,Biology ,lcsh:RC321-571 ,03 medical and health sciences ,0302 clinical medicine ,Calcium flux ,medicine ,lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry ,Neuroinflammation ,Original Research ,Retina ,Microglia ,hypoxia ,General Neuroscience ,Neurodegeneration ,neurodegeneration ,medicine.disease ,Cell biology ,030104 developmental biology ,medicine.anatomical_structure ,inflammation ,Neuroglia ,Muller glia ,030217 neurology & neurosurgery ,Neuroscience ,calcium spiking - Abstract
The detailed mechanisms underlying oxidative stress that leads to neuroinflammation and neurodegeneration in retinal vascular conditions, including diabetic retinopathy, retinopathy of prematurity etc., remain largely unexplored mainly due to a lack of suitable disease models that can simulate the inherent neuron–glia interactions in human retina. Specifically, establishment of a mixed retinal culture (MRC) containing both neuron and glial cell types remains a challenge due to different conditions required for their optimal growth and differentiation. Here, we establish a novel primary MRC model system containing neurons, astrocytes, Müller glia, and microglia from human donor retina that can be used to study the neuromodulatory effects of glial cells under the stress. The cell characterization based on immunostaining with individual cell type–specific markers and their presence in close vicinity to each other further underscores their utility for studying their cross talk. To the best of our knowledge, this is the first instance of an in vitro model obtained from human donor retina containing four major cell types. Next, we induce hypoxic stress to MRC to investigate if hypoxia activated neuroglia modulates altered gene expression for inflammatory, apoptotic, and angiogenic markers and Ca2+ transients by live cell imaging. Further, we performed k-means clustering of the Ca2+ responses to identify the modification of clustering pattern in stressed condition. Finally, we provide the evidence that the altered Ca2+ transient correlates to differential expression of genes shown to be involved in neuroinflammation, angiogenesis, and neurodegeneration under the hypoxic conditions as seen earlier in human cell lines and animal models of diabetic retinopathy. The major features of the hypoxic conditions in the proposed human MRC model included: increase in microglia activity, chemokine and cytokine expression, and percentage of cells having higher amplitude and frequency of Ca2+ transients. Thus, the proposed experimental system can potentially serve as an ideal in vitro model for studying the neuroinflammatory and neurodegenerative changes in the retina and identifying newer drug targets.
- Published
- 2020
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