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From the rallying call of the USA's Black Lives Matter movement, three Australian university teacher educators present perspectives on First Nations dance in the school curriculum. The Australian education system has emerged from the devastating impact of European colonisation upon the continent's First Nations peoples resulting in trauma, resistance, and resilience. Theory/praxis approaches to matters of Indigenous marginalisation within the school system are presented in relation to the context of public interest in "truth telling" about past colonial injustices. We draw first upon genealogical research to track the prohibition of Aboriginal dance in schools from the early years of colonisation to the later "White Australia" policy until now. Next, the complexities of embedding a new Aboriginal and Torres Strait Islander Histories and Cultures' Cross-Curriculum Priority are considered. Third, we explain research into teachers' enactment of First Nations dance in schools. Finally, a summary suggests ways forward from past wrongs.

Abstract Disorders of gallbladder motility can lead to serious pathology. Bitter tastants acting upon bitter taste receptors (TAS2R family) have been proposed as a novel class of smooth muscle relaxants to combat excessive contraction in the airways and other organs. To explore whether this might also emerge as an option for gallbladder diseases, we here tested bitter tastants for relaxant properties and profiled Tas2r expression in the mouse gallbladder. In organ bath experiments, the bitter tastants denatonium, quinine, dextromethorphan, and noscapine, dose-dependently relaxed the pre-contracted gallbladder. Utilizing gene-deficient mouse strains, neither transient receptor potential family member 5 (TRPM5), nor the Tas2r143/Tas2r135/Tas2r126 gene cluster, nor tuft cells proved to be required for this relaxation, indicating direct action upon smooth muscle cells (SMC). Accordingly, denatonium, quinine and dextromethorphan increased intracellular calcium concentration preferentially in isolated gallbladder SMC and, again, this effect was independent of TRPM5. RT-PCR revealed transcripts of Tas2r108, Tas2r126, Tas2r135, Tas2r137, and Tas2r143, and analysis of gallbladders from mice lacking tuft cells revealed preferential expression of Tas2r108 and Tas2r137 in tuft cells. A TAS2R143-mCherry reporter mouse labeled tuft cells in the gallbladder epithelium. An in silico analysis of a scRNA sequencing data set revealed Tas2r expression in only few cells of different identity, and from in situ hybridization histochemistry, which did not label distinct cells. Our findings demonstrate profound tuft cell- and TRPM5-independent relaxing effects of bitter tastants on gallbladder smooth muscle, but do not support the concept that these effects are mediated by bitter receptors.

Abstract Here, we show that combined use of the EZH2 inhibitor GSK126 and the CDK4/6 inhibitor abemaciclib synergistically enhances antitumoral effects in preclinical GBM models. Dual blockade led to HIF1α upregulation and CalR translocation, accompanied by massive impairment of mitochondrial function. Basal oxygen consumption rate, ATP synthesis, and maximal mitochondrial respiration decreased, confirming disrupted endoplasmic reticulum-mitochondrial homeostasis. This was paralleled by mitochondrial depolarization and upregulation of the UPR sensors PERK, ATF6α, and IRE1α. Notably, dual EZH2/CDK4/6 blockade also reduced 3D-spheroid invasion, partially inhibited tumor growth in ovo, and led to impaired viability of patient-derived organoids. Mechanistically, this was due to transcriptional changes in genes involved in mitotic aberrations/spindle assembly (Rb, PLK1, RRM2, PRC1, CENPF, TPX2), histone modification (HIST1H1B, HIST1H3G), DNA damage/replication stress events (TOP2A, ATF4), immuno-oncology (DEPDC1), EMT-counterregulation (PCDH1) and a shift in the stemness profile towards a more differentiated state. We propose a dual EZH2/CDK4/6 blockade for further investigation.

Abstract The major risk factor for chronic disease is chronological age, and age-related chronic diseases account for the majority of deaths worldwide. Targeting senescent cells that accumulate in disease-related tissues presents a strategy to reduce disease burden and to increase healthspan. The senolytic combination of the tyrosine-kinase inhibitor dasatinib and the flavonol quercetin is frequently used in clinical trials aiming to eliminate senescent cells. Here, our goal was to computationally identify natural senotherapeutic repurposing candidates that may substitute dasatinib based on their similarity in gene expression effects. The natural senolytic piperlongumine (a compound found in long pepper), and the natural senomorphics parthenolide, phloretin and curcumin (found in various edible plants) were identified as potential substitutes of dasatinib. The gene expression changes underlying the repositioning highlight apoptosis-related genes and pathways. The four compounds, and in particular the top-runner piperlongumine, may be combined with quercetin to obtain natural formulas emulating the dasatinib + quercetin formula.

A neurocysticercosis-like lesion in an 11-year-old boy in the Netherlands was determined to be caused by the zoonotic Taenia martis tapeworm. Subsequent testing revealed that 15% of wild martens tested in that region were infected with T. martis tapeworms with 100% genetic similarity; thus, the infection source was most likely local.

Chokeberries, which belong to the rose family (Rosaceae), have received increasing research attention due to their high content of secondary metabolites, especially oligomeric proanthocyanidins (OPCs). OPC-rich extracts are attributed to various positive health effects, including antioxidant or anti-inflammatory properties, which is why they are sold as food supplements. However, knowledge about the antioxidant properties of single OPCs is quite limited. Several separation steps with different separation techniques were performed to isolate OPCs from a pre-produced extract. More than 90 analytes were detected in the enriched fractions, which include eight OPCs, four cinchonains and one hexoside, including their respective isomers. For the characterization of the OPCs, high-resolution mass spectrometry coupled with liquid chromatography (LC-HRMS) was used. Based on the fragment spectra of the MS2 experiments, conclusions about the fragmentation pathways and the structure of six new OPCs could be drawn. After isolating trimers, tetramers and pentamers, it was possible to test the antioxidant effect in relation to the individual degrees of polymerization (DP) or structures. The Trolox-equivalent antioxidant capacity (TEAC) test showed that all OPCs investigated exhibit antioxidant effects and a first correlation between the antioxidant effect and the DP could be postulated, which suggests new possibilities for the design of food supplements.

Manipulating the motion of individual trapped ions at the single quantum level has become standard practice in radio-frequency ion traps, enabling sweeping advances in quantum information processing and precision metrology. The key step for motional-state engineering is ground-state cooling. Full motional control also bears great potential to explore another regime of sensitivities for fundamental physics tests in Penning traps. Here we demonstrate the key enabling step by implementing resolved-sideband cooling on the axial mode of a single ^{9}Be^{+} ion in a 5 Tesla cryogenic Penning trap. The system has been developed for the implementation of high-precision antimatter experiments to test the fundamental symmetries of the standard model with the highest accuracy in the baryonic sector. We measure an axial phonon number of n[over ¯]_{z}=0.10(4) after cooling and demonstrate that the axial heating rate in our system is compatible with the implementation of quantum logic spectroscopy of (anti-)protons.

This qualitative study examined the mathematics self-confidence of eight junior high school students who were moved to an above-grade-level mathematics class through a nontraditional process. Teachers were concerned about how this transition may impact students' beliefs about their abilities to succeed in mathematics. Data were collected through interviews that included solving challenging mathematical tasks as a means to consider how students expressed their self-confidence in mathematics in general. Using a socio-constructivist lens with a focus on mediation, findings included themes about tensions given students' initial placement, changes in the role of self-confidence as a mediator, feelings of belonging as having multiple mediator roles, workarounds as mediators, and self-regulation strategies as internalized mediators that students brought with them to their transition. These findings point to solutions and supports for students who enroll in above-grade-level courses to view themselves as successful.

The Translational Science Working Group at the European Respiratory Society (ERS) aims to bridge the gap between basic and clinical science by providing a platform where scientists, clinicians and experts in the respiratory field can actively shape translational research. For the 2023 Congress, dedicated translational science sessions were created and sessions of interest to many assemblies from the clinical and the scientific point of view were tagged as translational sessions, attracting clinical and scientific experts to the same room to discuss relevant topics and strengthening translational efforts among all ERS assemblies.

User experience (UX) is a holistic concept. We conceptualize UX as a set of semantically distinct quality aspects. These quality aspects relate subjectively perceived properties of the user interaction with a product to the psychological needs of users. Not all possible UX quality aspects are equally important for all products. The main use case of a product can determine the relative importance of UX aspects for the overall impression of the UX. In this paper, the authors present several studies that investigate this dependency between the product category and the importance of several well-known UX aspects. A method to measure the importance of such UX aspects is presented. In addition, the authors show that the observed importance ratings are stable, i.e., reproducible, and hardly influenced by demographic factors or cultural background. Thus, the ratings reported in our studies can be reused by UX professionals to find out which aspects of UX they should concentrate on in product design and evaluation.

Histone post-translational modifications (PTMs) play a critical role in chromatin regulation. It has been proposed that these PTMs form localized ‘codes’ that are read by specialized regions (reader domains) in chromatin-associated proteins (CAPs) to regulate downstream function. Substantial effort has been made to define [CAP: histone PTM] specificities, and thus decipher the histone code and guide epigenetic therapies. However, this has largely been done using the reductive approach of isolated reader domains and histone peptides, which cannot account for any higher-order factors. Here, we show that the [BPTF PHD finger and bromodomain: histone PTM] interaction is dependent on nucleosome context. The tandem reader selectively associates with nucleosomal H3K4me3 and H3K14ac or H3K18ac, a combinatorial engagement that despite being in cis is not predicted by peptides. This in vitro specificity of the BPTF tandem reader for PTM-defined nucleosomes is recapitulated in a cellular context. We propose that regulatable histone tail accessibility and its impact on the binding potential of reader domains necessitates we refine the ‘histone code’ concept and interrogate it at the nucleosome level.

Background/Context: Although the uprisings in the summer of 2020 amplified existing abolitionist organizing, including abolitionist struggles for justice within K-12 schools, it is unclear if the field of teacher education has been informed by these movements. Purpose/Objective/Research Question/Focus of Study: Given this gap, as well as the ongoing urgency to dismantle the interconnected structures of White supremacy, settler colonialism, anti-Blackness, racial capitalism, and cis-heteropatriarchy within the field of teacher education, this article calls on teacher education programs and teacher educators to grow abolition within the field. Research Design: We situate abolition broadly and demonstrate the connections between abolition and struggles for justice in K-12 education. We draw on stories and lessons from our own work as educators and organizers to situate what must be dismantled, changed, and built to grow abolition within the field of teacher education. Conclusions/Recommendations: We call on teacher education programs and teacher educators to begin the reflexive, relational, embodied, imaginative, coalitional, urgent, and necessary work of growing abolition within the field of teacher education.

Fascinating 3D shapes arise when a thin planar sheet is folded without stretching, tearing or cutting. The elegance amplifies when the fold/crease is changed from a straight line to a curve, due to the association of plastic deformation via folding and elastic deformation via bending. This results in the curved crease working as a hinge support providing deployability to the surface which is of significant interest in industrial engineering and architectural design. Consequently, finding a stable form of curved crease becomes pivotal in the development of deployable structures. This work proposes a novel way to evaluate such curves by taking inspiration from biomimicry. For this purpose, growth mechanism in plants was observed and an analogous model was developed to create a discrete curve of fold. A parametric model was developed for digital construction of the folded models. Test cases were formulated to compare the behavior of different folded models under various loading conditions. A simplified way to visualize the obtained results is proposed using visual programming tools. The models were further translated into physical prototypes with the aid of 3D printing, hybrid and cured-composite systems, where different mechanisms were adopted to achieve the folds. The prototypes were further tested under constrained boundary and compressive loading conditions, with results validating the analytical model.

Contemporary schooling produces unequal educational outcomes in Australia and across the globe. While mandated high-stakes tests supposedly place all students on a common scale, they can limit pedagogic practices and often fail to recognize the "abilities" or embodied knowledge of many children. In addressing these challenges, particularly as they relate to the teaching of mathematics this article reports on a qualitative study that investigated an arts integrated professional learning model, Creative Body-based Learning (CBL), at two Australian primary schools. CBL uses active and creative strategies from a range of art forms to increase student engagement and expand pedagogic possibilities across the curriculum. In this pilot study, five teachers formed action research teams with four artists to integrate CBL into mathematics. Findings drawn from interviews with teachers include higher engagement and improvement of student dispositions in mathematics and, more significantly, a broadening of teachers' pedagogical practices to engage students and provide them with multiple opportunities to present their learning.

Contact formation and autohesion with respect to their role as the major mechanisms governing the tack between thermoset prepregs in automated fiber placement were explored. Therefore, a novel 90° peel test with strictly separated and individually controllable compaction and debonding phases was employed for experimental tack characterization in a rheometer. Variation of compaction pressure, dwell time and temperature enabled the experimental isolation of contact formation and autohesion influences. The experimentally determined tack, ply-ply contact area and resin viscoelastic characteristics were used to parametrize simplified semi-empirical bond strength sub-models that have originally been developed for thermoplastic composite manufacturing techniques. The model prediction was validated successfully within the experimentally reproducible parameter range. Eventually, manufacturing scenarios for thermoset automated fiber placement (AFP) respecting different lay-up velocities (up to 1 m s−1), compaction pressures (up to 10 N mm−2) and both lay-up and mold temperatures (20–60 °C) were assessed in terms of estimated prepreg tack. The implication of both mechanisms, contact formation and autohesion, in the evolution of prepreg tackiness was found to be able to replicate the bell-shaped tack curves proposed by the adhesion-cohesion balance.

This paper examines the personal and professional experiences of the five arts leaders who co-wrote the foundation document for Australia's first national curriculum in the Arts. Their personal and professional backgrounds, which were explored during in depth interviews, drove the complex collaborative process that informed the first iteration of the "Australian Curriculum: The Arts." Though each couched their responses in the context of their background and arts discipline, they shared an awareness of the important role of the Arts in providing the analytical tools for children and young people to identify and subsequently challenge social injustice. The findings, which are presented as a group narrative using a Narrative Inquiry approach, reveal how the five arts leaders' individual lived experience, disciplinary experience and expertise, and commitment to collaborative leadership informed their approach. It was one driven by their shared belief that all Australian students, regardless of their background, are entitled to a quality arts education.

The paper begins by drawing upon research to understand the genealogical position of dance within the school curriculum as a new 'entitlement' for all young Australians. Whilst dance is included within the Australian curriculum it has been historically marginalised as a 'soft' subject within curriculum hierarchy. This low position in the curriculum order has been questioned, with assertions that dance requires a high level of embodied intelligence not usually recognised by education systems. Ball's (1993) analytic framework for policy-making has been utilised to explore the context of influence through a genealogy of the dance curriculum; the context of policy text production through the process of writing about Dance for the Arts Shape paper in the Australian Curriculum; and the context of policy practice through a case study of pre-service teachers' lived experiences of dance in school. The findings revealed a series of complex factors which have impacted upon the implementation and practice of dance in schools including the relationship between teacher control, disciplinary power and student agency; the impact of dance competitions and their influence on the perceptions of dance; the interests of diverse faiths and cultures as well as the potential of dance to support growing interest in Aboriginal and Torres Strait Islander histories and culture; and the twenty-first century proliferation of dance on screen via new technologies and access to highly sexualised performance are discussed. These factors prompt the need for critical approaches towards developing a more accessible and socially just dance curriculum that is culturally responsive and meaningful for diverse learners.

Introduction Type 2 diabetes mellitus (T2DM) is a powerful risk factor for cardiovascular disease (CVD), conferring a greater relative risk in women than men. We sought to examine sex differences in cardiometabolic risk factors and management in the contemporary cohort represented by the Glycemia Reduction Approaches in Diabetes: A Comparative Effectiveness Study (GRADE).Research design and methods GRADE enrolled 5047 participants (1837 women, 3210 men) with T2DM on metformin monotherapy at baseline. The current report is a cross-sectional analysis of baseline data collected July 2013 to August 2017.Results Compared with men, women had a higher mean body mass index (BMI), greater prevalence of severe obesity (BMI≥40 kg/m2), higher mean LDL cholesterol, greater prevalence of low HDL cholesterol, and were less likely to receive statin treatment and achieve target LDL, with a generally greater prevalence of these risk factors in younger women. Women with hypertension were equally likely to achieve blood pressure targets as men; however, women were less likely to receive ACE inhibitors or angiotensin receptor blockers. Women were more likely to be divorced, separated or widowed, and had fewer years of education and lower incomes.Conclusions This contemporary cohort demonstrates that women with T2DM continue to have a greater burden of cardiometabolic and socioeconomic risk factors than men, particularly younger women. Attention to these persisting disparities is needed to reduce the burden of CVD in women.Trial registration number ClinicalTrials.gov (NCT01794143)

We employ renewal processes to characterize the spatiotemporal dynamics of an active Brownian particle under stochastic orientational resetting. By computing the experimentally accessible intermediate scattering function (ISF) and reconstructing the full time-dependent distribution of the displacements, we study the interplay of rotational diffusion and resetting. The resetting process introduces a new spatiotemporal regime reflecting the directed motion of agents along the resetting direction at large length scales, which becomes apparent in an imaginary part of the ISF. We further derive analytical expressions for the low-order moments of the displacements and find that the variance displays an effective diffusive regime at long times, which decreases for increasing resetting rates. At intermediate times the dynamics are characterized by a negative skewness as well as a non-zero non-Gaussian parameter.

Abstract Reflecting the first wave COVID-19 pandemic in Central Europe (i.e. March 16th–April 15th, 2020) the neurosurgical community witnessed a general diminution in the incidence of emergency neurosurgical cases, which was impelled by a reduced number of traumatic brain injuries (TBI), spine conditions, and chronic subdural hematomas (CSDH). This appeared to be associated with restrictions imposed on mobility within countries but also to possible delayed patient introduction and interdisciplinary medical counseling. In response to one year of COVID-19 experience, also mapping the third wave of COVID-19 in 2021 (i.e. March 16 to April 15, 2021), we aimed to reevaluate the current prevalence and outcomes for emergency non-elective neurosurgical cases in COVID-19-negative patients across Austria and the Czech Republic. The primary analysis was focused on incidence and 30-day mortality in emergency neurosurgical cases compared to four preceding years (2017–2020). A total of 5077 neurosurgical emergency cases were reviewed. The year 2021 compared to the years 2017–2019 was not significantly related to any increased odds of 30 day mortality in Austria or in the Czech Republic. Recently, there was a significant propensity toward increased incidence rates of emergency non-elective neurosurgical cases during the third COVID-19 pandemic wave in Austria, driven by their lower incidence during the first COVID-19 wave in 2020. Selected neurosurgical conditions commonly associated with traumatic etiologies including TBI, and CSDH roughly reverted to similar incidence rates from the previous non-COVID-19 years. Further resisting the major deleterious effects of the continuing COVID-19 pandemic, it is edifying to notice that the neurosurgical community´s demeanor to the recent third pandemic culmination keeps the very high standards of non-elective neurosurgical care alongside with low periprocedural morbidity. This also reflects the current state of health care quality in the Czech Republic and Austria.

In nucleosomes, histone N-terminal tails exist in dynamic equilibrium between free/accessible and collapsed/DNA-bound states. The latter state is expected to impact histone N-termini availability to the epigenetic machinery. Notably, H3 tail acetylation (e.g. K9ac, K14ac, K18ac) is linked to increased H3K4me3 engagement by the BPTF PHD finger, but it is unknown if this mechanism has a broader extension. Here, we show that H3 tail acetylation promotes nucleosomal accessibility to other H3K4 methyl readers, and importantly, extends to H3K4 writers, notably methyltransferase MLL1. This regulation is not observed on peptide substrates yet occurs on the cis H3 tail, as determined with fully-defined heterotypic nucleosomes. In vivo, H3 tail acetylation is directly and dynamically coupled with cis H3K4 methylation levels. Together, these observations reveal an acetylation ‘chromatin switch’ on the H3 tail that modulates read-write accessibility in nucleosomes and resolves the long-standing question of why H3K4me3 levels are coupled with H3 acetylation.

The increased effectiveness of antiretroviral therapy (ART) in the last 30 years is a scientific landmark, and viral suppression is directly associated with treatment adherence. The aim of this study was to compare the results of ART adherence and viral load suppression with the evolution of the protocols and other associated factors, in people living with HIV. A panel analysis of three descriptive longitudinal studies investigating ART adherence and viral load suppression was conducted in people with HIV treated at a drug dispensing unit in the Federal District. The studies were carried out during periods of 2011, 2013, and 2017, coinciding with the three different recommended treatment schemes for the country. Adherence was assessed using drug dispensing records. Viral load data were obtained from the Ministry of Healthʼs Laboratory Examination Information System. Analysis of the data of 522 individuals in the three periods showed sociodemographic differences such as a decline in the percentage of women (from 33% in period 1 to 4% in period 3) and an increase in the percentage of young people. ART adherence was higher in period 2 (tenofovir/lamivudine/efavirenz scheme). Viral load suppression was greater in period 3 (tenofovir/lamivudine/dolutegravir scheme). The relative detectable viral load risk was nearly two-fold higher (RR 1.83) in people living with HIV with less than 80% adherence when compared to those above 80%. With respect to the different schemes recommended in Brazil during the periods studied, ART containing dolutegravir was the most effective in achieving viral load suppression. By contrast, there was better ART adherence in the daily combined fixed dose consisting of tenofovir/lamivudine/efavirenz in tablet form. Adherence to ART above 80% seemed to be enough to promote an effective treatment in therapeutic schemes including efavirenz or dolutegravir.

Assuming a dialectical approach to technology and pedagogy, this study explores sensemaking processes for instructors teaching in a technologically enhanced college classroom environment. Through a series of semi-structured individual and group interviews, seven instructors provided narrative accounts of the problems encountered with progressive instructional technology and their emergent strategies to make sense of and manage it. Three primary dialectical tensions were described: freedom vs. confinement, connectedness vs. fragmentation, and change vs. stability. Two related modes of sensemaking in response to these tensions were also uncovered: adaptation, involving day-to-day adjustments to non-routine failures, and reframing, entailing gradual reflection upon the instructors' roles in the classroom. Implications for the current findings are discussed.

We demonstrate optical sideband spectroscopy of a single ^{9}Be^{+} ion in a cryogenic 5 tesla Penning trap using two-photon stimulated-Raman transitions between the two Zeeman sublevels of the 1s^{2}2s ground state manifold. By applying two complementary coupling schemes, we accurately measure Raman resonances with and without contributions from motional sidebands. From the latter we obtain an axial sideband spectrum with an effective mode temperature of (3.1±0.4) mK. These results are a key step for quantum logic operations in Penning traps, applicable to high-precision matter-antimatter comparison tests in the baryonic sector of the standard model.

What can white people do to resist racism and white supremacy in this political moment? Using the conceptual frameworks of transformative justice and community accountability drawn from antiviolence and antiprison industrial complex movements, this essay explores this question and aims to contribute to the proliferation of collective action. Building from and with examples from organizers, activists and theorists who are working collectively, often within the curricular and cocurricular spaces of education and schools, to challenge white supremacy and other forms of oppression, this essay offers partial suggestions and engagements to build the capacity of white people to dismantle white supremacy.

Aerospace-grade prepreg resin films based on multifunctional tetraglycidyl-4,4′-methylenedianiline (TGMDA), triglycidyl p-aminophenol (TGAP), Bisphenol A diglycidyl ether (DGEBA) and curing agent 4,4'diaminodiphenyl sulfone (DDS) are investigated in terms of tackiness by probe testing. The model epoxy systems are modified regarding the thermoplastic toughener content (polyethersulfone, PES) and the B-stage level, which is adjusted by cure prediction based on a model-free isoconversional method (Flynn-Wall-Ozawa). Additional DSC and rheological analysis are performed to study the thermal and viscoelastic material behavior in conjunction to its impact on temperature-dependent tack. Maximum achievable tack is found to decrease as a function of both degree of conversion and toughener content. Meanwhile, both influencing factors shift the tack maximum towards higher temperatures corresponding to increased flow characteristics attributed to evolving network formation and the incorporation of high molecular weight PES. In terms of absolute tack level and corresponding temperature, probe tack values similar to commercial prepreg systems (∼100 μJ mm−2) are recorded for TGMDA-based formulations containing 10 wt% PES at 20% pre-cure. Model formulations, which have neither been exposed to B-staging nor toughened, show exceptionally high tack below room temperature for all investigated epoxy prepolymers and are therefore not considered processable by automated fiber placement.

The majority of peptides presented by MHC class I result from proteasomal protein turnover. The specialized immunoproteasome, which is induced during inflammation, plays a major role in antigenic peptide generation. However, other cellular proteases can, either alone or together with the proteasome, contribute peptides to MHC class I loading non-canonically. We used an immunopeptidomics workflow combined with prediction software for proteasomal cleavage probabilities to analyze how inflammatory conditions affect the proteasomal processing of immune epitopes presented by A549 cells. The treatment of A549 cells with IFNγ enhanced the proteasomal cleavage probability of MHC class I ligands for both the constitutive proteasome and the immunoproteasome. Furthermore, IFNγ alters the contribution of the different HLA allotypes to the immunopeptidome. When we calculated the HLA allotype-specific proteasomal cleavage probabilities for MHC class I ligands, the peptides presented by HLA-A*30:01 showed characteristics hinting at a reduced C-terminal proteasomal cleavage probability independently of the type of proteasome. This was confirmed by HLA-A*30:01 ligands from the immune epitope database, which also showed this effect. Furthermore, two additional HLA allotypes, namely, HLA-A*03:01 and HLA-A*11:01, presented peptides with a markedly reduced C-terminal proteasomal cleavage probability. The peptides eluted from all three HLA allotypes shared a peptide binding motif with a C-terminal lysine residue, suggesting that this lysine residue impairs proteasome-dependent HLA ligand production and might, in turn, favor peptide generation by other cellular proteases.