Search

A crucial aspect of constructing a gridded model of anthropogenic fossil fuel CO2 (FFCO2) emissions involves careful consideration of uncertainty. Both the spatial resolution of the emissions estimates (grid scale) and the selection of proxy data to represent the spatial distribution of emissions, plus the quality of data on point sources of emissions, have important impacts on uncertainty. In earlier papers, we explored the uncertainties associated with grid selection and the available data on large point sources. In this work CO2 emissions data are spatially distributed using population density as the selected proxy, using three different treatments of large point sources, and with five levels of grid resolution (1o, 2o, 3o, 4o, and 5o). The methods of calculating uncertainty associated with grid size, proxy selection, and reported point-source emissions data are presented, with particular attention being drawn to grid size selection. We find that as the resolution becomes coarser, relative uncertainty (total uncertainty as a percentage of total emissions) at the grid cell level decreases. Relative uncertainty in most grid cells decreases as the portion of emissions attributed to specific point sources increases. Good data on large point sources is very important for spatially explicit emissions inventories. [ABSTRACT FROM AUTHOR]

Aim: To evaluate how pain, during and in-between dressing changes, is affected by the introduction of Mepilex® Border Lite, a wound dressing manufactured by Molnlycke Health Care using Safetac® soft silicone adhesive technology, to the treatment of different types of paediatric wounds/skin injuries.Methods: Wounds/skin injuries that met the criteria for inclusion in the study were dressed with Mepilex® Border Lite. Patients were followed for six weeks or until their wounds/skin injuries had healed, whichever occurred earlier. At each dressing change, pain severity before and during dressing removal was rated by the patient and the investigator on a scale from zero (no pain at all) to ten (worst pain ever). Other variables measured included: signs of trauma to wound/skin injury and surrounding skin, the proportion of viable/non-viable tissue, the quantity and appearance of exudate, odour, and clinical signs of infection. At the final dressing change, patients and investigators completed questionnaires to rate the dressing performance.Results: Mean pain severity scores were significantly lower (p < or = 0.003) at the first dressing change than at baseline. Over 99.5 per cent of the Mepilex® Border Lite dressing changes were reported to be atraumatic and more than half of the wounds healed within the study period. Conformability, ease of use, ease or removal, patient comfort, and overall experience with the dressing were rated as 'good' to 'very good' at the vast majority of final visit evaluations.Conclusions: This study provides further evidence of the ability of dressings with Safetac soft silicone adhesive technology to minimise trauma and pain and demonstrates the ability of Mepilex® Border Lite to overcome the clinical challenges associated with the use of dressings on the wounds/skin injuries of paediatric patients. [ABSTRACT FROM AUTHOR]

A total of 1080 individual patient samples (158 positive serology samples from confirmed, predominantly mildly symptomatic COVID-19 patients and 922 serology negative including 496 collected pre-COVID) from four states in Australia were analysed on four commercial SARS-CoV-2 serological assays targeting antibodies to different antigens (Roche Elecsys and Abbott Architect: nucleocapsid; Diasorin Liaison and Euroimmun: spike). A subset was compared to immunofluorescent antibody (IFA) and micro-neutralisation. Sensitivity and specificity of the Roche (n = 1033), Abbott (n = 806), Diasorin (n = 1034) and Euroimmun (n = 175) were 93.7 %/99.5 %, 90.2 %/99.4 %, 88.6 %/98.6 % and 91.3 %/98.8 %, respectively. ROC analysis with specificity held at 99 % increased the sensitivity for the Roche and Abbott assays from 93.7% to 98.7% (cut-off 0.21) and 90.2 % to 94.0 % (cut-off 0.91), respectively. Overall seropositivity of samples increased from a maximum of 23 % for samples 0-7 days-post-onset of symptoms (dpos), to 61 % from samples 8-14dpos and 93 % from those >14dpos. IFA and microneutralisation values correlated best with assays targeting antibodies to spike protein with values >80 AU/mL on the Diasorin assay associated with neutralising antibody. Detectable antibody was present in 22/23 (96 %), 20/23 (87 %), 15/23 (65 %) and 9/22 (41 %) patients with samples >180dpos on the Roche, Diasorin, Abbott and microneutralisation assays respectively. Given the low prevalence in this community, two-step algorithms on initial positive results saw an increase in the positive predictive value (PPV) of positive samples (39 %-65 % to ≥98 %) for all combinations. Similarly accuracy increased from a range of 98.5 %-99.4 % to ≥99.8 % assuming a 1 % seroprevalence. Negative predictive value (NPV) was high (≥99.8 %) regardless of which assay was used initially., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Background: Although there is broad agreement that negative carbon emissions may be required in order to meet the global climate change targets specified in the Paris Agreement and that carbon sequestration in the terrestrial biosphere can be an important contributor, there are important accounting issues that often discourage forest carbon sequestration projects. The legislation establishing the California forest offset program, for example, requires that offsets be "real, additional, quantifiable, permanent, verifiable, and enforceable". While these are all clearly desirable attributes, their implementation has been a great challenge in balancing complexity, expense, and risk. Most forest offset protocols carry similar accounting objectives, but often with different details, (e.g. Richards and Huebner in Carbon Manag 3(4):393-410, 2012 and Galik et al. in Mitig Adapt Strateg Glob Change 14:677-690, 2009). The result is that the complexity, expense, and risk of participation discourage participation and make it more difficult to achieve climate mitigation goals. We focus on the requirements for accounting and permanence to illustrate that current requirements disproportionately disadvantage small landowners., Results: The simplified 1040EZ filing system for U.S. income taxes may provide insight for a protocol model that balances reward, effort, and risk, while still achieving the overall objectives of standardized offset protocols. In this paper, we present initial ideas and lay the groundwork behind a "2050EZ" protocol for forest carbon sequestration as a complement to existing protocols., Conclusion: The Paris Agreement states that "Parties should take action to conserve and enhance, as appropriate, sinks and reservoirs of greenhouse gases." The Paris Agreement also refers to issues such as equity, sustainable development, and other non-carbon benefits. The challenge is to provide incentives for maintaining and increasing the amount of carbon sequestered in the biosphere. Monitoring and verification of carbon storage need to be sufficient to demonstrate sequestration from the atmosphere while providing clear incentives and simple accounting approaches that encourage participation by diverse participants, including small land holders.

The aim of this article is to explore the concept of medical futility and the withdrawal of care for children in intensive care units. There have been several recent cases where medical staff have considered that there was no possibility of recovery for a child, yet their clinical judgments were challenged by the parents. The private anguish of these families became public, social media heightened emotions and this was followed by political and religious intrusion. Innovations in medical treatment and technological advances raise issues for all those involved in the care of children and young people especially when decisions need to be made about end of life care. Healthcare professionals have a moral and legal obligation to determine when treatment should cease in cases where it is determined to be futile. The aim should be to work collaboratively with parents but all decisions must be made in the best interests of the child. However, medical staff and parents may have differing opinions about care decisions. In part, this may be as a result of their unique relationships with the child and different understanding of the extent to which the child is in discomfort or can endure pain., Competing Interests: None declared, (©2019 RCN Publishing Company Ltd. All rights reserved. Not to be copied, transmitted or recorded in any way, in whole or part, without prior permission of the publishers.)

Carbon dioxide (CO 2 ) emissions from U.S. power plants are independently reported by the U.S. Energy Information Administration (EIA) and the Clean Air Markets Division (CAMD) within the U.S. Environmental Protection Agency (EPA). Differences between the CAMD and EIA emission tallies show that the amount of CO 2 produced by an individual power plant is less certain than might be imagined or desired. These differences are attributed to systematic error and random measurement error. Random error cannot be retroactively corrected, whereas systematic error can be corrected where relevant data are available. Accordingly, this study identified and, where possible, corrected systematic error affecting the CAMD and EIA CO 2 emission tallies for 1065 power plants that emitted more than 25,000 tons of CO 2 during 2013. The EIA tallies were corrected by accounting for emission factor error, acid-gas sorbent consumption, and combustion of biogenic fuel. The CAMD tallies were likewise corrected by accounting for unreported unit emissions. It was not possible to objectively correct systematic error affecting about 11% of the power plants, and subjective corrections were not attempted. At these plants, the CAMD and EIA emission tallies sometimes differed by more than 20% due to missing unit error, plant identification error, temporal measurement error, or inferred reporting error. Comparisons of the CAMD and EIA emission tallies before and after correction for systematic error show the effectiveness of these corrections. The comparisons also show the persistence of random measurement error. Implications: Understanding the uncertainty of CO2 emission tallies for USA power plants might inform emission inventories, atmospheric flow models or inversions, and emission reduction policies. Knowing the cause and size of measurement errors that contribute to this uncertainty might also help to identify ways to improve the measurement methods and reporting protocols that these CO2 emission tallies are based on.

Although the evolutionary significance of gene duplication has long been appreciated, it remains unclear what factors determine gene duplicability. In this study we investigated whether metabolism is an important determinant of gene duplicability because cellular metabolism is crucial for the survival and reproduction of an organism. Using genomic data and metabolic pathway data from the yeast (Saccharomyces cerevisiae) and Escherichia coli, we found that metabolic proteins indeed tend to have higher gene duplicability than nonmetabolic proteins. Moreover, a detailed analysis of metabolic pathways in these two organisms revealed that genes in the central metabolic pathways and the catabolic pathways have, on average, higher gene duplicability than do other genes and that most genes in anabolic pathways are single-copy genes.

Comparative genomic analysis at its most fundamental level involves alignment and analysis of linear strings of DNA. Many useful and powerful tools, such as BlastN and ClustalW are able to respectively, search for, and align similar strings of DNA from a variety of species. However, interesting genomic patterns cannot be immediately visualized within the information contact embedded in long genomic strings without extensive a priori knowledge. More problematic is the question of whether we will be able to crystallize long genomic sequences and analyze their true secondary and tertiary structures. It is, of course, these putative motifs that are binding to the three-dimensional structures of proteins and inducing replication and transcription events. The W-curve is a numerical mapping algorithm that allows one to geometrically visualize the information content of genomic motifs. Patterns of ALU, LINES, SINEs, and duplication sequences may be easily visualized with the W-curve. It is our hope that this pattern recognition algorithm will lead to visualization tools to track the evolutionary history of motif patterns. The combinatorics of DNA motif crossover-recombination events will be more easily followed as we continue to sequence more and more genomes. In our laboratory we are currently collaborating with mathematicians and computer scientists to develop and test tools, such as the W-curve, for analyzing patterns of long genomic sequences. In this paper, we examine the limitations of using the W-curve to infer the phylogenetic history of species.