1. Monoclonal Antibodies Applied in an Immunoperoxidase Method for Detection of Parvovirus in Specimens of Small Intestine from Dog and Mink
- Author
-
Lars Jönsson, Cajsa Magnusson, MajLis Book, and Nils Juntti
- Subjects
Hemagglutination ,medicine.drug_class ,viruses ,Monoclonal antibody ,Article ,Parvoviridae ,Immunoenzyme Techniques ,Parvoviridae Infections ,Dogs ,biology.animal ,Intestine, Small ,medicine ,Animals ,Dog Diseases ,Mink ,General Veterinary ,biology ,Immunoperoxidase ,Parvovirus ,Canine parvovirus ,virus diseases ,Antibodies, Monoclonal ,General Medicine ,biology.organism_classification ,Virology ,Molecular biology ,Small intestine ,medicine.anatomical_structure ,Polyclonal antibodies ,biology.protein - Abstract
Virus isolation and the haemagglutination test are traditionally used for detection of parvovirus in faeces and intestinal contents (Weber 1983). In 1980 Osterhaus et al demonstrated parvovirus-like particles in faeces of dogs by means of negative contrast electron microscopy. Lately, ELISA tests have been developed for the detection of canine parvovirus (Mildbrand 1984, Fiscus 1984, Juntti et al, in manuscript). Further, the presence of parvovirus in histological specimens, fixed either in formalin-sublimate (Macartney & Macartney 1986) or in 10% buffered formalin (Ducatelle et al. 1981), has been demonstrated using polyclonal antibodies and the immunoperoxidase technique.
- Published
- 1988