42 results on '"Macierzanka, Adam"'
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2. The bile salt/phospholipid ratio determines the extent of in vitro intestinal lipolysis of triglycerides: Interfacial and emulsion studies
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Kłosowska, Katarzyna, del Castillo-Santaella, Teresa, Maldonado-Valderrama, Julia, and Macierzanka, Adam
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- 2024
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3. Effect of oxidation and in vitro intestinal hydrolysis on phospholipid toxicity towards HT29 cell line serving as a model of human intestinal epithelium
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Parchem, Karol, Baranowska, Monika, Kościelak, Anna, Kłosowska-Chomiczewska, Ilona, Domingues, M. Rosário, Macierzanka, Adam, and Bartoszek, Agnieszka
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- 2023
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4. Relative quantification of pork and beef in meat products using global and species-specific peptide markers for the authentication of meat composition
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Nalazek-Rudnicka, Katarzyna, Kłosowska-Chomiczewska, Ilona E., Brockmeyer, Jens, Wasik, Andrzej, and Macierzanka, Adam
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- 2022
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5. INFOGEST inter-laboratory recommendations for assaying gastric and pancreatic lipases activities prior to in vitro digestion studies
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Grundy, Myriam M.L., Abrahamse, Evan, Almgren, Annette, Alminger, Marie, Andres, Ana, Ariëns, Renata M.C., Bastiaan-Net, Shanna, Bourlieu-Lacanal, Claire, Brodkorb, André, Bronze, Maria R., Comi, Irene, Couëdelo, Leslie, Dupont, Didier, Durand, Annie, El, Sedef N., Grauwet, Tara, Heerup, Christine, Heredia, Ana, Infantes Garcia, Marcos R., Jungnickel, Christian, Kłosowska-Chomiczewska, Ilona E., Létisse, Marion, Macierzanka, Adam, Mackie, Alan R., McClements, David J., Menard, Olivia, Meynier, Anne, Michalski, Marie-Caroline, Mulet-Cabero, Ana-Isabel, Mullertz, Anette, Payeras Perelló, Francina M., Peinado, Irene, Robert, Mélina, Secouard, Sébastien, Serra, Ana T., Silva, Sandra D., Thomassen, Gabriel, Tullberg, Cecilia, Undeland, Ingrid, Vaysse, Carole, Vegarud, Gerd E., Verkempinck, Sarah H.E., Viau, Michelle, Zahir, Mostafa, Zhang, Ruojie, and Carrière, Frédéric
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- 2021
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6. Bile salts in digestion and transport of lipids
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Macierzanka, Adam, Torcello-Gómez, Amelia, Jungnickel, Christian, and Maldonado-Valderrama, Julia
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- 2019
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7. MRM–MS of marker peptides and their abundance as a tool for authentication of meat species and meat cuts in single-cut meat products
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Nalazek-Rudnicka, Katarzyna, Kłosowska-Chomiczewska, Ilona, Wasik, Andrzej, and Macierzanka, Adam
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- 2019
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8. INFOGEST static in vitro simulation of gastrointestinal food digestion
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Brodkorb, André, Egger, Lotti, Alminger, Marie, Alvito, Paula, Assunção, Ricardo, Ballance, Simon, Bohn, Torsten, Bourlieu-Lacanal, Claire, Boutrou, Rachel, Carrière, Frédéric, Clemente, Alfonso, Corredig, Milena, Dupont, Didier, Dufour, Claire, Edwards, Cathrina, Golding, Matt, Karakaya, Sibel, Kirkhus, Bente, Le Feunteun, Steven, Lesmes, Uri, Macierzanka, Adam, Mackie, Alan R., Martins, Carla, Marze, Sébastien, McClements, David Julian, Ménard, Olivia, Minekus, Mans, Portmann, Reto, Santos, Cláudia N., Souchon, Isabelle, Singh, R. Paul, Vegarud, Gerd E., Wickham, Martin S. J., Weitschies, Werner, and Recio, Isidra
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- 2019
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9. Comparing the permeability of human and porcine small intestinal mucus for particle transport studies
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Krupa, Lukasz, Bajka, Balazs, Staroń, Robert, Dupont, Didier, Singh, Harjinder, Gutkowski, Krzysztof, and Macierzanka, Adam
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- 2020
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10. Permeability of the small intestinal mucus for physiologically relevant studies: Impact of mucus location and ex vivo treatment
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Macierzanka, Adam, Mackie, Alan R., and Krupa, Lukasz
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- 2019
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11. Sodium alginate decreases the permeability of intestinal mucus
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Mackie, Alan R., Macierzanka, Adam, Aarak, Kristi, Rigby, Neil M., Parker, Roger, Channell, Guy A., Harding, Stephen E., and Bajka, Balazs H.
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- 2016
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12. The influence of small intestinal mucus structure on particle transport ex vivo
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Bajka, Balázs H., Rigby, Neil M., Cross, Kathryn L., Macierzanka, Adam, and Mackie, Alan R.
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- 2015
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13. The effect of gel structure on the kinetics of simulated gastrointestinal digestion of bovine β-lactoglobulin
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Macierzanka, Adam, Böttger, Franziska, Lansonneur, Laura, Groizard, Rozenn, Jean, Anne-Sophie, Rigby, Neil M., Cross, Kathryn, Wellner, Nikolaus, and Mackie, Alan R.
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- 2012
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14. Enzymatic cross-linking of β-lactoglobulin in solution and at air–water interface: Structural constraints
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Ercili-Cura, Dilek, Partanen, Riitta, Husband, Fiona, Ridout, Mike, Macierzanka, Adam, Lille, Martina, Boer, Harry, Lantto, Raija, Buchert, Johanna, and Mackie, Alan R.
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- 2012
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15. Standardization of in vitro digestibility and DIAAS method based on the static INFOGEST protocol
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Egger, Lotti, Sousa, Raquel, Recio, Isidra, Abbühl, Lychou, Brügger, Cédric, Santos-Hernández, Marta, Macierzanka, Adam, Szumała, Patrycja, Rama, Addepalli, Mulet-Cabero, Ana-Isabel, Perez-Moral, Natalia, Brodkorb, Andre, Fitzpatrick, Conor, Rieder, Anne, Levi, Carmit Shani, Gonçales, Catarina, Rodriguez-Amado, Isabel, Nobre, Clarisse, Pinheiro Canton, Ana, Arranz, Elena, Bot, Francesca, A. O'Mahony, James, Grundy, Myriam M.-L., Pacheco-Cruz, Igor, Sepúlveda-González, Ailynne, Comi, Irene, Abrankó, László, Tormási, Judit, Gonzales, Felipe, Ruas-Madiedo, Patricia, Ruiz, Lorena, Faria, Miguel-Ângelo, Sobral, M.Madalena.C, Jiménez-Munoz, Luis Miguel, Corredig, Milena, Jimenez, Luis, Molly, Muleya, Rosa-Sibakov, Natalia, Ménard, Olivia, Orlien, Vibeke, Gousetti, Ourania, Lykkepetersen, Iben, Ferranti, Pasquale, Olias, Raquel, Delgado Andrade, Cristina, Assunção, Ricardo, López-Nicolás, Rubén, Simsek, Sebnem, Karakaya, Sibel, El, Sedef Nehir, Rezzi, Serge, Canarelli, Stéphane, Petit, Valérie, Emre Tuncil, Yunus, Hotrum, Nathalie, Broersen, Kerensa, Heimo, Dominique, Dubois, Sebastien, Portmann, Reto, and Giboulot, Anne
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[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition ,In vitro ,food martrice ,Digestion ,Ethic ,Amino acid assimilation - Abstract
The FAO recommends the digestible indispensable amino acid score (DIAAS) as the measure for protein quality, for which the true ileal digestibility needs to be assessed in humans or pigs. However, due to high costs and ethical concerns, the FAO strongly encourages as well the development of validated in vitro methods, which complement the in vivo experiments. Method: Recently, an in vitro workflow, based on the validated static INFOGEST protocol, was developed and compared towards in vivo data. In parallel to the validation with in vivo data, the repeatability and reproducibility of the in vitro protocol were tested in an international ring trial (RT) with the aim to establish an international ISO standard method within the International Dairy Federation (IDF). Five different dairy products (skim milk powder, whole milk powder, whey protein isolate, yoghurt, and cheese) were analyzed in 32 different laboratories from 18 different countries, across 4 continents. Results: in vitro protein digestibilities based on Nitrogen, free R-NH2, and total amino acids as well as DIAAS values were calculated and compared to in vivo data, where available. Conclusion: The in vitro method is suited for quantification of digestibility and will be further implemented to other food matrices.
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- 2022
16. The role of bile salts in digestion
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Maldonado-Valderrama, Julia, Wilde, Pete, Macierzanka, Adam, and Mackie, Alan
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- 2011
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17. Colloidal aspects of protein digestion
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Mackie, Alan and Macierzanka, Adam
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- 2010
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18. Effect of crystalline emulsifier composition on structural transformations of water-in-oil emulsions: Emulsification and quiescent conditions
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Macierzanka, Adam, Szeląg, Halina, Szumała, Patrycja, Pawłowicz, Roman, Mackie, Alan R., and Ridout, Michael J.
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- 2009
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19. Microstructural behavior of water-in-oil emulsions stabilized by fatty acid esters of propylene glycol and zinc fatty acid salts
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Macierzanka, Adam and Szeląg, Halina
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- 2006
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20. Which casein in sodium caseinate is most resistant to in vitro digestion? Effect of emulsification and enzymatic structuring
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Böttger, Franziska, Dupont, Didier, Marcinkowska, Dorota, Bajka, Balazs, Mackie, Alan, and Macierzanka, Adam
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- 2019
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21. Which casein in sodium caseinate is most resistant to in vitro digestion? Effect of 2 emulsification and enzymatic structuring
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Böttger, Franziska, Dupont, Didier, Marcinkowska, Dorota, Bajka, Balazs, Mackie, Alan R, and Macierzanka, Adam
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Sodium Caseinate ,Emulsion ,Casein ,Digestion ,ELISA ,Transglutaminase - Abstract
We investigated the resistance of individual constituent casein epitopes (αS1-, αS2-, β- and κ-CN) in food-grade milk protein sodium caseinate (NaCN) to simulated human gastro-duodenal digestion. The influence of NaCN adsorption to the surface of oil-in-water emulsion droplets and the effect of crosslinking of the protein with enzyme transglutaminase (TG) on the proteolysis were studied by indirect ELISA. TG crosslinking rendered fragments of casein molecules significantly resistant to digestion. However, it depended on the type of casein and whether NaCN was presented in solution or emulsion. The crosslinking was found to considerably hinder the digestion of several amino acid regions in one of the major caseins of NaCN, β-CN. For αS1- and αS2-CN, only limited resistance to digestive enzymes was observed after NaCN had been crosslinked in solution but not (or to a limited extent) in emulsion. κ-CN proved to be the least resistant to the enzymatic hydrolysis regardless of the TG treatment. Our work shows for the first time how the digestibility of individual components of important food-grade protein ingredients can differ in a complex, colloidal food system. It also shows an example of how the digestibility can be modulated by chemical and physical structuring.
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- 2018
22. Transport of food digesta and model particles in the small intestinal mucus
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MACIERZANKA, Adam, Mackie, A.R., Dupont, Didier, Nau, Francoise, Ménard, Olivia, Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institute of Food Research [Norwich], COST Action FA 1005 INFOGEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), and Institut National de Recherche Agronomique (INRA). UMR Science et Technologie du Lait et de l'Oeuf (1253).
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surface épithéliale ,obésité ,nutriment ,[SDV.IDA]Life Sciences [q-bio]/Food engineering ,digestion ,santé ,nanoparticule ,[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition ,mucus intestinal - Abstract
The last boundary between ingested food and the gastrointestinal (GI) tract mucosa is the mucus layer. This highly complex viscoelastic medium has evolved to provide a robust barrier that traps and immobilises potentially hazardous particulates such as bacteria but still allows the passage of nutrients to the epithelial surfaces. However, the rules governing this selective barrier function, particularly in relation to transport of particulates, remain unknown. Recent studies suggest that surface properties of model nanoparticles can impact on colloidal transport in intestinal mucus (1). In particular, adsorption of bile salts (BS) to particles proved to significantly enhance their diffusion in the small intestinal mucus in vitro (2). The concentration of BS in the human small intestinal lumen can, however, vary greatly between the fasted and the fed states. There is also a significant difference in the overall concentration of BS between different age groups (e.g. infant vs. adult humans). In this study we used quantitative confocal microscopy to look at the transport characteristics in the porcine ex vivo (detached from the tissue) intestinal mucus of post-digestion food particulates such as yogurt digesta as well as lipid droplets that derived from in vitro digestion of sodium caseinate stabilised emulsions. Moreover, we used multiple-particle tracking technique to investigate the extent to which the concentration of BS can impact on the permeability of the mucus to model, sub-micron size particles such as latex beads, and their transport in this secretion. Finally, we investigated how the permeability and microstructure of the intact small intestinal mucus (i.e. attached to the mucosal tissue) may vary between specific locations on the tissue. Such insights will help support efforts to both engineer fabricated foods with enhanced nutritional quality required to fight the obesity epidemic, and to overcome the failure of many pharmaceutical and nutraceutical preparations to provide effective oral delivery of active compounds.
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- 2015
23. Colloidal transport in the intestinal mucus
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Macierzanka, Adam, Mackie, Alan, Dupont, Didier, Nau, Francoise, Institute of Food Research [Norwich], Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut National de la Recherche Agronomique (INRA), and Cost Infogest
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surface épithéliale ,nutriment ,mucus ,intestin ,transport colloidal ,digestive, oral, and skin physiology ,[SDV.IDA]Life Sciences [q-bio]/Food engineering ,colloide intestinal transport mucus surface micronutriment barrière santé ,santé humaine ,digestion ,[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology - Abstract
The last boundary between ingested food and the gastrointestinal (GI) tract mucosa is the mucus barrier. This highly complex viscoelastic medium has evolved to provide a robust barrier that can trap and immobilise potentially hazardous particulates such as bacteria but still allow the passage of nutrients to the epithelial surfaces (1,2). These conflicting properties are particularly important in the small intestine where the mucus layer is thinnest and the majority of nutrients absorption takes place. However, the rules governing this selective barrier function, particularly in relation to transport of particulates, remain unknown. Recent studies show that surface properties of microparticles can largely impact on colloidal transport in the intestinal mucus (3). The physico-chemical characteristics of the absorption of nutrients depend on the structure of post-digestion food particles which, in turn, is determined by the original food structure and the kinetics and pattern of its enzymatic breakdown in the GI tract. Detailed characterisation of both, the colloidal aspects of digestion and the transport of post-digestion food particles in the intestinal mucus is required for rational design of foods with controlled behaviour in the GI tract, improving their nutritional quality and maintaining human health, thus overcoming diet-related health problems. Here, we summarise our recent studies on the effect the structure of intestinal mucus on the rate of colloidal transport in the mucus.
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- 2013
24. Approaches to Static Digestion Models.
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Mackie, Alan, Rigby, Neil, Macierzanka, Adam, and Bajka, Balazs
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- 2015
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25. Cross-linking of sodium caseinate-structured emulsion with transglutaminase alters postprandial metabolic and appetite responses in healthy young individuals.
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Juvonen, Kristiina R., Macierzanka, Adam, Lille, Martina E., Laaksonen, David E., Mykkänen, Hannu M., Niskanen, Leo K., Pihlajamäki, Jussi, Mäkelä, Kari A., Mills, Clare E. N., Mackie, Alan R., Malcolm, Paul, Herzig, Karl-Heinz, Poutanen, Kaisa S., and Karhunen, Leila J.
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APPETITE ,BLOOD sugar ,CASEINS ,CHOLECYSTOKININ ,CROSSOVER trials ,EMULSIONS ,FAT ,FATTY acids ,GASTROINTESTINAL motility ,INGESTION ,INSULIN ,LONGITUDINAL method ,METABOLISM ,PROBABILITY theory ,PSYCHOLOGICAL tests ,QUESTIONNAIRES ,RESEARCH funding ,STATISTICS ,TRANSFERASES ,TRIGLYCERIDES ,GLUCAGON-like peptide 1 ,DATA analysis ,VISUAL analog scale ,BLIND experiment ,DATA analysis software ,DESCRIPTIVE statistics - Abstract
The physico-chemical and interfacial properties of fat emulsions influence lipid digestion and may affect postprandial responses. The aim of the present study was to determine the effects of the modification of the interfacial layer of a fat emulsion by cross-linking on postprandial metabolic and appetite responses. A total of fifteen healthy individuals (26·5 (sem 6·9) years and BMI 21·9 (sem 2·0) kg/m2) participated in a cross-over design experiment in which they consumed two isoenergetic (1924 kJ (460 kcal)) and isovolumic (250 g) emulsions stabilised with either sodium caseinate (Cas) or transglutaminase-cross-linked sodium caseinate (Cas-TG) in a randomised order. Blood samples were collected from the individuals at baseline and for 6 h postprandially for the determination of serum TAG and plasma NEFA, cholecystokinin (CCK), glucagon-like peptide 1 (GLP-1), glucose and insulin responses. Appetite was assessed using visual analogue scales. Postprandial TAG and NEFA responses and gastric emptying (GE) rates were comparable between the emulsions. CCK increased more after the ingestion of Cas-TG than after the ingestion of Cas (P< 0·05), while GLP-1 responses did not differ between the two test emulsions. Glucose and insulin profiles were lower after consuming Cas-TG than after consuming Cas (P< 0·05). The overall insulin, glucose and CCK responses, expressed as areas above/under the curve, did not differ significantly between the Cas and Cas-TG meal conditions. Satiety ratings were reduced and hunger, desire to eat and thirst ratings increased more after the ingestion of Cas-TG than after the ingestion of Cas (P< 0·05). The present results suggest that even a subtle structural modification of the interfacial layer of a fat emulsion can alter the early postprandial profiles of glucose, insulin, CCK, appetite and satiety through decreased protein digestion without affecting significantly on GE or overall lipid digestion. [ABSTRACT FROM PUBLISHER]
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- 2015
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26. Specificity of Infant Digestive Conditions: Some Clues for Developing Relevant In Vitro Models.
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Bourlieu, Claire, Ménard, Olivia, Bouzerzour, Karima, Mandalari, Giuseppina, Macierzanka, Adam, Mackie, Alan R., and Dupont, Didier
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INFANT physiology ,INFANT nutrition ,IN vitro studies ,PREMATURE infants ,POSTNATAL care ,DIFFERENCES - Abstract
Digestion of nutrients is an essential function of the newborn infant gut to allow growth and development and understanding infant digestive function is essential to optimize nutrition and oral drug delivery. Ethical considerations prohibit invasive in vivo trials and as a consequence in vitro assays are often conducted. However, the choice of in vitro model parameters are not supported by an exhaustive analysis of the literature and do not mimic precisely the digestive conditions of the infant. This review contains a compilation of the studies which characterized the gastroduodenal conditions in full-term or preterm infants of variable postnatal age from birth up to six months. Important data about healthy full-term infants are reported. The enzymatic (type of enzymes and level of activity) and nonenzymatic (milk-based diet, frequency of feeding, bile salt concentrations) conditions of digestion in infants are shown to differ significantly from those in adults. In addition, the interindividual and developmental variability of the digestive conditions in infants is also highlighted. [ABSTRACT FROM AUTHOR]
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- 2014
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27. Transport of Particles in Intestinal Mucus under Simulated Infant and Adult Physiological Conditions: Impact of Mucus Structure and Extracellular DNA.
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Macierzanka, Adam, Mackie, Alan R., Bajka, Balazs H., Rigby, Neil M., Nau, Françoise, and Dupont, Didier
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MUCUS , *EXTRACELLULAR fluid , *SMALL intestine physiology , *PEDIATRIC gastroenterology , *CONFOCAL microscopy , *EPITHELIAL cells - Abstract
The final boundary between digested food and the cells that take up nutrients in the small intestine is a protective layer of mucus. In this work, the microstructural organization and permeability of the intestinal mucus have been determined under conditions simulating those of infant and adult human small intestines. As a model, we used the mucus from the proximal (jejunal) small intestines of piglets and adult pigs. Confocal microscopy of both unfixed and fixed mucosal tissue showed mucus lining the entire jejunal epithelium. The mucus contained DNA from shed epithelial cells at different stages of degradation, with higher amounts of DNA found in the adult pig. The pig mucus comprised a coherent network of mucin and DNA with higher viscosity than the more heterogeneous piglet mucus, which resulted in increased permeability of the latter to 500-nm and 1-µm latex beads. Multiple-particle tracking experiments revealed that diffusion of the probe particles was considerably enhanced after treating mucus with DNase. The fraction of diffusive 500-nm probe particles increased in the pig mucus from 0.6% to 64% and in the piglet mucus from ca. 30% to 77% after the treatment. This suggests that extracellular DNA can significantly contribute to the microrheology and barrier properties of the intestinal mucus layer. To our knowledge, this is the first time that the structure and permeability of the small intestinal mucus have been compared between different age groups and the contribution of extracellular DNA highlighted. The results help to define rules governing colloidal transport in the developing small intestine. These are required for engineering orally administered pharmaceutical preparations with improved delivery, as well as for fabricating novel foods with enhanced nutritional quality or for controlled calorie uptake. [ABSTRACT FROM AUTHOR]
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- 2014
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28. Topical delivery of pharmaceutical and cosmetic macromolecules using microemulsion systems.
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Szumała, Patrycja and Macierzanka, Adam
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MICROEMULSIONS , *MACROMOLECULES , *COLLOIDS , *MOLECULAR weights , *PHARMACOKINETICS , *BIOLOGICAL transport - Abstract
[Display omitted] Microemulsions are transparent, thermodynamically stable colloidal systems. Over the recent years, they have been increasingly investigated due to their potential as skin delivery vehicles for a wide range of drug molecules. The nanoscale particle size and the specificity of microemulsion components are the main features determining the skin permeation process. However, in order to effectively cross the skin barrier, the active substance itself should also meet a number of requirements, such as relatively small molecular weight, high lipophilicity with certain polarity as well as a specific partition coefficient. This review focuses on recent advancements in topical microemulsion systems related to the transport of active ingredients into the skin, including those with high molecular weight and high polarity. Selected studies have shown that permeation of therapeutic macromolecules can be increased by the correct (i.e. tailored to a specific drug) design of the microemulsion. The degree of skin penetration as well as the kinetics and the site of drug release can be controlled by appropriate qualitative and quantitative selections of penetration promoters (microemulsion components), the structure of microemulsion and its viscosity. The drug-carrier interactions can also affect the effectiveness of microemulsion formulation. These relations have been described and evaluated in this review article. [ABSTRACT FROM AUTHOR]
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- 2022
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29. Analysis of the Factors Affecting Static In Vitro Pepsinolysis of Food Proteins.
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Maeda, Natsumi, Dulko, Dorota, Macierzanka, Adam, and Jungnickel, Christian
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PEPSIN ,FACTOR analysis ,PROTEOLYSIS ,CYTOSKELETAL proteins ,PROTEINS ,NUTRITIONAL requirements - Abstract
In this meta-analysis, we collected 58 publications spanning the last seven decades that reported static in vitro protein gastric digestion results. A number of descriptors of the pepsinolysis process were extracted, including protein type; pepsin activity and concentration; protein concentration; pH; additives; protein form (e.g., 'native', 'emulsion', 'gel', etc.); molecular weight of the protein; treatment; temperature; and half-times (HT) of protein digestion. After careful analysis and the application of statistical techniques and regression models, several general conclusions could be extracted from the data. The protein form to digest the fastest was 'emulsion'. The rate of pepsinolysis in the emulsion was largely independent of the protein type, whereas the gastric digestion of the native protein in the solution was strongly dependent on the protein type. The pepsinolysis was shown to be strongly dependent on the structural components of the proteins digested—specifically, β-sheet-inhibited and amino acid, leucine, methionine, and proline-promoted digestion. Interestingly, we found that additives included in the digestion mix to alter protein hydrolysis had, in general, a negligible effect in comparison to the clear importance of the protein form or additional treatment. Overall, the findings allowed for the targeted creation of foods for fast or slow protein digestion, depending on the nutritional needs. [ABSTRACT FROM AUTHOR]
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- 2022
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30. Lamellar Structures ofMUC2-Rich Mucin:A Potential Role in Governing the Barrier and Lubricating Functionsof Intestinal Mucus.
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Round, Andrew N., Rigby, Neil M., Garcia de la Torre, Angela, Macierzanka, Adam, Mills, E. N. Clare, and Mackie, Alan R.
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- 2012
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31. Transglutaminase cross-linking kinetics of sodium caseinate is changed after emulsification
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Macierzanka, Adam, Bordron, François, Rigby, Neil M., Mills, E.N. Clare, Lille, Martina, Poutanen, Kaisa, and Mackie, Alan R.
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TRANSGLUTAMINASES , *MILK proteins , *EMULSIONS , *CASEINS , *SODIUM , *TRIGLYCERIDES - Abstract
Abstract: Enzymatic cross-linking is an important method of modifying the structure of food products to control their texture and stability. In this paper we look at the effect that adsorption to the oil–water interface of triglyceride oil-in-water emulsion has on rates of cross-linking of sodium caseinate by microbial transglutaminase. The kinetics of cross-linking has also been assessed for the individual casein proteins within the caseinate. In solution the rates were αs2-casein>β-casein>αs1-casein>κ-casein. This order is not as expected given the rheomorphic nature of the proteins and the number of glutamine and lysine residues in each protein. In particular, the αs1-casein was cross-linked much more slowly than expected. When sodium caseinate was adsorbed to an emulsion the rates for all constituent caseins were decreased but the cross-linking rate for αs1-casein was markedly reduced, indicating the most significant change in accessibility following adsorption. This knowledge will facilitate optimal production of cross-linked emulsions for use in future studies aimed at engineering emulsions with improved nutritional quality. [Copyright &y& Elsevier]
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- 2011
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32. Emulsification alters simulated gastrointestinal proteolysis of β-casein and β-lactoglobulin.
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Macierzanka, Adam, Sancho, Ana I., Clare Mills, E. N., Rigby, Neil M., and Mackie, Alan R.
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- 2009
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33. Importance of Bile Composition for Diagnosis of Biliary Obstructions.
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Krupa, Łukasz, Staroń, Robert, Dulko, Dorota, Łozińska, Natalia, Mackie, Alan R., Rigby, Neil M., Macierzanka, Adam, Markiewicz, Aleksandra, and Jungnickel, Christian
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BILE ,BILE salts ,ENDOSCOPIC retrograde cholangiopancreatography ,RECEIVER operating characteristic curves ,SODIUM cholate ,BLOOD serum analysis - Abstract
Determination of the cause of a biliary obstruction is often inconclusive from serum analysis alone without further clinical tests. To this end, serum markers as well as the composition of bile of 74 patients with biliary obstructions were determined to improve the diagnoses. The samples were collected from the patients during an endoscopic retrograde cholangiopancreatography (ERCP). The concentration of eight bile salts, specifically sodium cholate, sodium glycocholate, sodium taurocholate, sodium glycodeoxycholate, sodium chenodeoxycholate, sodium glycochenodeoxycholate, sodium taurodeoxycholate, and sodium taurochenodeoxycholate as well as bile cholesterol were determined by HPLC-MS. Serum alanine aminotransferase (ALT), aspartate transaminase (AST), and bilirubin were measured before the ERCP. The aim was to determine a diagnostic factor and gain insights into the influence of serum bilirubin as well as bile salts on diseases. Ratios of conjugated/unconjugated, primary/secondary, and taurine/glycine conjugated bile salts were determined to facilitate the comparison to literature data. Receiver operating characteristic (ROC) curves were determined, and the cut-off values were calculated by determining the point closest to (0,1). It was found that serum bilirubin was a good indicator of the type of biliary obstruction; it was able to differentiate between benign obstructions such as choledocholithiasis (at the concentration of >11 µmol/L) and malignant changes such as pancreatic neoplasms or cholangiocarcinoma (at the concentration of >59 µmol/L). In addition, it was shown that conjugated/unconjugated bile salts confirm the presence of an obstruction. With lower levels of conjugated/unconjugated bile salts the possibility for inflammation and, thus, neoplasms increase. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
34. Properties of W/O Emulsions Stabilized with Acylglycerol Emulsifiers Modified with Zinc Carboxylates.
- Author
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Szela¸g, Halina, Macierzanka, Adam, and Pawłowicz, Roman
- Subjects
- *
ZINC , *DISPERSION (Chemistry) , *PARAFFIN wax , *VEGETABLE oils , *EMULSIONS , *ESTERIFICATION , *FATTY acids , *CONDUCTOMETRIC analysis , *CALORIMETRY - Abstract
The stability of water/paraffin oil and some water/vegetable oil dispersions stabilized by acylglycerol emulsifiers modified with zinc carboxylates have been evaluated. The acylglycerol emulsifiers were prepared by esterification of glycerol with fatty acid in the presence of zinc fatty acid carboxylate. The modification of surface activity of the emulsifiers was obtained by using defined hydrocarbon chain lengths of monoacylglycerols and carboxylates. Using conductometric, microscopic and calorimetric (differential scanning calorimetry, DSC) methods, the influence of the following parameters on dispersion stability was studied: emulsifier composition and concentration, phase volume ratio, emulsion droplet diameter, emulsion stability index, and index of coalescence. [ABSTRACT FROM AUTHOR]
- Published
- 2004
- Full Text
- View/download PDF
35. Towards Rational Biosurfactant Design—Predicting Solubilization in Rhamnolipid Solutions.
- Author
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Kłosowska-Chomiczewska, Ilona E., Kotewicz-Siudowska, Adrianna, Artichowicz, Wojciech, Macierzanka, Adam, Głowacz-Różyńska, Agnieszka, Szumała, Patrycja, Mędrzycka, Krystyna, Hallmann, Elżbieta, Karpenko, Elena, Jungnickel, Christian, and Chrzanowski, Łukasz
- Subjects
BIOSURFACTANTS ,MOLECULAR volume ,SOLUBILIZATION ,SUPPORT vector machines ,EVOLUTIONARY algorithms - Abstract
The efficiency of micellar solubilization is dictated inter alia by the properties of the solubilizate, the type of surfactant, and environmental conditions of the process. We, therefore, hypothesized that using the descriptors of the aforementioned features we can predict the solubilization efficiency, expressed as molar solubilization ratio (MSR). In other words, we aimed at creating a model to find the optimal surfactant and environmental conditions in order to solubilize the substance of interest (oil, drug, etc.). We focused specifically on the solubilization in biosurfactant solutions. We collected data from literature covering the last 38 years and supplemented them with our experimental data for different biosurfactant preparations. Evolutionary algorithm (EA) and kernel support vector machines (KSVM) were used to create predictive relationships. The descriptors of biosurfactant (logP
BS , measure of purity), solubilizate (logPsol , molecular volume), and descriptors of conditions of the measurement (T and pH) were used for modelling. We have shown that the MSR can be successfully predicted using EAs, with a mean R2 val of 0.773 ± 0.052. The parameters influencing the solubilization efficiency were ranked upon their significance. This represents the first attempt in literature to predict the MSR with the MSR calculator delivered as a result of our research. [ABSTRACT FROM AUTHOR]- Published
- 2021
- Full Text
- View/download PDF
36. Colloidal transport of lipid digesta in human and porcine small intestinal mucus.
- Author
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Macierzanka, Adam, Ménard, Olivia, Dupont, Didier, Gutkowski, Krzysztof, Staroń, Robert, and Krupa, Lukasz
- Subjects
- *
MUCUS , *CONFOCAL microscopy , *LIPIDS , *PIGLETS , *LIPOLYSIS - Abstract
• Small intestinal mucus showed a sieve-like behaviour when exposed to lipid digesta. • Digesta penetration profiles of mucus are similar for adult human and adult pig. • Adult pig mucus is a human-relevant substitute for mucus transport studies. • Neonatal, piglet mucus is a less effective barrier to passively diffusing digesta. Small intestinal mucus transport of food-derived particulates has not been extensively studied, despite mucus being a barrier nutrients need to cross before absorption. We used complex dispersions of digesta obtained from simulated, dynamic gastrointestinal digestion of yogurt to examine the penetrability of human and porcine mucus to the particles formed of lipolysis products. Quantitative, time-lapse confocal microscopy revealed a sieve-like behaviour of the pig jejunal and ileal mucus. The digesta diffusivity decreased significantly over the first 30 min of mucus penetration, and then remained constant at ca. 5 × 10-12 m2 s−1 (approx. 70% decrease from initial values). A non-significantly different penetrability was recorded for the ileal mucus of adult humans. The digesta diffusion rates in neonatal, jejunal mucus of 2 week old piglets were 5–8 times higher than in the three different types of adult mucus. This is the first report that validates the mucus of fully-grown pigs as a human-relevant substitute for mucus permeation studies of nutrients/bio-actives and/or complex colloidal dispersions (e.g., post-digestion food particulates, orally-administrated delivery systems). [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
37. The bile salt content of human bile impacts on simulated intestinal proteolysis of β-lactoglobulin.
- Author
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Dulko, Dorota, Staroń, Robert, Krupa, Lukasz, Rigby, Neil M., Mackie, Alan R., Gutkowski, Krzysztof, Wasik, Andrzej, and Macierzanka, Adam
- Subjects
- *
LACTOGLOBULINS , *BILE salts , *PROTEOLYSIS , *INTESTINES , *SCIENTIFIC literature , *PROTEOLYTIC enzymes - Abstract
[Display omitted] • Bile salts (BS) accelerate gastrointestinal proteolysis of bovine milk βLg. • Composition of human bile (HB) impacts on the extent of βLg digestion. • BS content of HB is more important than its BS profile in facilitating proteolysis. • Impact of HB can be replicated by the use of individual BS with phosphatidylcholine. The gastrointestinal hydrolysis of food proteins has been portrayed in scientific literature to predominantly depend on the activity and specificity of proteolytic enzymes. Human bile has not been considered to facilitate proteolysis in the small intestine, but rather to assist in intestinal lipolysis. However, human bile can potentially influence proteins that are largely resistant to gastric digestion, and which are mainly hydrolysed after they have been transferred to the small intestine. We used purified and food-grade bovine milk β-lactoglobulin (βLg) to assess the impact of bile salts (BS) on the in vitro gastrointestinal digestion of this protein. Quantitative analysis showed that the proteolysis rate increased significantly with increasing BS concentration. The effect was consistent regardless of whether individual BS or real human bile samples, varying in BS concentrations, were used. The total BS content of bile was more important than its BS composition in facilitating the proteolysis of βlg. We also show that the impact of human bile observed during the digestion of purified βLg and βLg-rich whey protein isolate can be closely replicated by the use of individual BS mixed with phosphatidylcholine. This could validate simple BS/phosphatidylcholine mixtures as human-relevant substitutes of difficult-to-obtain human bile for in vitro proteolysis studies. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
38. Transport of Particles in Intestinal Mucus under Simulated Infant and Adult Physiological Conditions: Impact of Mucus Structure and Extracellular DNA
- Author
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Adam Macierzanka, Didier Dupont, Françoise Nau, Balazs Bajka, Alan R. Mackie, Neil M. Rigby, Macierzanka, Adam, Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institute of Food Research [Norwich], European Commission through the Universite Europeenne de Bretagne, France, and BBSRC through an Institute Strategic Programme [BB/J004545/1]
- Subjects
Aging ,modèle animal ,Physiology ,Polymers ,Digestive Physiology ,Sus scrofa ,aliment ,Ingénierie des aliments ,lcsh:Medicine ,digestion ,Pediatrics ,Physical Chemistry ,Biopolymers ,fluids and secretions ,Intestinal mucosa ,[SDV.IDA]Life Sciences [q-bio]/Food engineering ,Intestine, Small ,Medicine and Health Sciences ,Gastrointestinal Infections ,microscopie confocale ,mucus ,adn extracellulaire ,santé ,Intestinal Mucosa ,lcsh:Science ,Latex beads ,Multidisciplinary ,Viscosity ,Gastrointestinal Motility Disorders ,diffusion ,santé humaine ,Cell biology ,Chemistry ,medicine.anatomical_structure ,Infectious Diseases ,Physical Sciences ,Alimentation et Nutrition ,Small Intestine ,Pediatric Gastroenterology ,Anatomy ,Digestion ,Rheology ,Research Article ,Materials by Structure ,Materials Science ,Static Electricity ,Gastroenterology and Hepatology ,Biology ,mucus intestinal ,Microbiology ,transport intestinal ,Extracellular ,medicine ,Animals ,Food engineering ,Food and Nutrition ,Colloids ,Digestive Functions ,Mucin ,lcsh:R ,Biology and Life Sciences ,Biological Transport ,DNA ,Mucus ,Small intestine ,Gastrointestinal Tract ,Permeability (electromagnetism) ,Mixtures ,lcsh:Q ,Extracellular Space ,[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition ,Digestive System - Abstract
The final boundary between digested food and the cells that take up nutrients in the small intestine is a protective layer of mucus. In this work, the microstructural organization and permeability of the intestinal mucus have been determined under conditions simulating those of infant and adult human small intestines. As a model, we used the mucus from the proximal (jejunal) small intestines of piglets and adult pigs. Confocal microscopy of both unfixed and fixed mucosal tissue showed mucus lining the entire jejunal epithelium. The mucus contained DNA from shed epithelial cells at different stages of degradation, with higher amounts of DNA found in the adult pig. The pig mucus comprised a coherent network of mucin and DNA with higher viscosity than the more heterogeneous piglet mucus, which resulted in increased permeability of the latter to 500-nm and 1-µm latex beads. Multiple-particle tracking experiments revealed that diffusion of the probe particles was considerably enhanced after treating mucus with DNase. The fraction of diffusive 500-nm probe particles increased in the pig mucus from 0.6% to 64% and in the piglet mucus from ca. 30% to 77% after the treatment. This suggests that extracellular DNA can significantly contribute to the microrheology and barrier properties of the intestinal mucus layer. To our knowledge, this is the first time that the structure and permeability of the small intestinal mucus have been compared between different age groups and the contribution of extracellular DNA highlighted. The results help to define rules governing colloidal transport in the developing small intestine. These are required for engineering orally administered pharmaceutical preparations with improved delivery, as well as for fabricating novel foods with enhanced nutritional quality or for controlled calorie uptake.
- Published
- 2014
39. Unresectable malignant obstructive jaundice: a 2-year experience of EUS-guided biliary drainage.
- Author
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Staroń R, Rzucidło M, Macierzanka A, Krawczyk M, Gutkowski K, and Krupa L
- Abstract
Objectives: Endoscopic biliary drainage is a first-line treatment in patients with unresectable malignant biliary obstruction. In most cases the drainage is conducted using endoscopic retrograde cholangiopancreatography (ERCP). Percutaneous transhepatic biliary drainage or endosonography-guided biliary drainage (EUS-BD) represents therapeutic options after unsuccessful ERCP. Here we report on 2 years experience in the management of patients diagnosed with malignant biliary obstruction using EUS-BD., Methods: Retrospective data were collected on patients who underwent EUS-BD due to malignant biliary obstruction at our centre between April 2016 and April 2018. Only patients who had two unsuccessful attempts of ERCP prior to EUS-BD were included. We analysed the technical success (ie, creation of anastomosis and successful placement of a stent) and complication rate of EUS-BD, and monitored changes in serum bilirubin and liver function tests after 2 days, and at least 2 weeks, following the procedure., Results: Screening of 1781 ERCP procedures performed in our department during the inclusion period led to the identification of 31 patients (18 women, age range 51-92 years, 58% with pancreatic cancer) who fulfilled the inclusion criteria. Hepaticogastrostomy and choledochoduodenostomy were performed in 12 and 19 patients, respectively. The technical success rate was 97% and the complication rate was 12.9%. EUS-BD resulted in a significant decrease in serum bilirubin (p<0.01)., Conclusions: EUS-BD represents a reasonable therapeutic option after unsuccessful ERCP in patients with malignant biliary obstruction. Possible complications have to be kept in mind and this procedure should be performed at centres experienced in ERCP and EUS., Competing Interests: Competing interests: None declared., (© Author(s) (or their employer(s)) 2021. No commercial re-use. See rights and permissions. Published by BMJ.)
- Published
- 2021
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40. Enzymatically structured emulsions in simulated gastrointestinal environment: impact on interfacial proteolysis and diffusion in intestinal mucus.
- Author
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Macierzanka A, Böttger F, Rigby NM, Lille M, Poutanen K, Mills EN, and Mackie AR
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- Animals, Caseins pharmacology, Chelating Agents pharmacology, Duodenum metabolism, Emulsions, Gastric Mucosa metabolism, Humans, Models, Biological, Swine, Transglutaminases chemistry, Caseins chemistry, Caseins pharmacokinetics, Chelating Agents chemistry, Chelating Agents pharmacokinetics, Drug Delivery Systems, Intestinal Mucosa metabolism, Proteolysis
- Abstract
Fundamental knowledge of physicochemical interactions in the gastrointestinal environment is required in order to support rational designing of protein-stabilized colloidal food and pharmaceutical delivery systems with controlled behavior. In this paper, we report on the colloidal behavior of emulsions stabilized with the milk protein sodium caseinate (Na-Cas), and exposed to conditions simulating the human upper gastrointestinal tract. In particular, we looked at how the kinetics of proteolysis was affected by adsorption to an oil-water interface in emulsion and whether the proteolysis and the emulsion stability could be manipulated by enzymatic structuring of the interface. After cross-linking with the enzyme transglutaminase, the protein was digested with use of an in vitro model of gastro-duodenal proteolysis in the presence or absence of physiologically relevant surfactants (phosphatidylcholine, PC; bile salts, BS). Significant differences were found between the rates of digestion of Na-Cas cross-linked in emulsion (adsorbed protein) and in solution. In emulsion, the digestion of a population of polypeptides of M(r) ca. 50-100 kDa was significantly retarded through the gastric digestion. The persistent interfacial polypeptides maintained the original emulsion droplet size and prevented the system from phase separating. Rapid pepsinolysis of adsorbed, non-cross-linked Na-Cas and its displacement by PC led to emulsion destabilization. These results suggest that structuring of emulsions by enzymatic cross-linking of the interfacial protein may affect the phase behavior of emulsion in the stomach and the gastric digestion rate in vivo. Measurements of ζ-potential revealed that BS displaced the remaining protein from the oil droplets during the simulated duodenal phase of digestion. Diffusion of the postdigestion emulsion droplets through ex vivo porcine intestinal mucus was only significant in the presence of BS due to the high negative charge these biosurfactants imparted to the droplets. This implies that the electrostatic repulsion produced can prevent the droplets from being trapped by the mucus matrix and facilitate their transport across the small intestine mucosal barrier.
- Published
- 2012
- Full Text
- View/download PDF
41. Lamellar structures of MUC2-rich mucin: a potential role in governing the barrier and lubricating functions of intestinal mucus.
- Author
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Round AN, Rigby NM, Garcia de la Torre A, Macierzanka A, Mills EN, and Mackie AR
- Subjects
- Animals, Cell Line, Tumor, Humans, Jejunum chemistry, Microscopy, Atomic Force, Models, Molecular, Molecular Structure, Mucin-2 isolation & purification, Swine, Intestinal Mucosa chemistry, Intestinal Mucosa metabolism, Mucin-2 chemistry
- Abstract
Mucus is a ubiquitous feature of mammalian wet epithelial surfaces, where it lubricates and forms a selective barrier that excludes a range of particulates, including pathogens, while hosting a diverse commensal microflora. The major polymeric component of mucus is mucin, a large glycoprotein formed by several MUC gene products, with MUC2 expression dominating intestinal mucus. A satisfactory answer to the question of how these molecules build a dynamic structure capable of playing such a complex role has yet to be found, as recent reports of distinct layers of chemically identical mucin in the colon and anomalously rapid transport of nanoparticles through mucus have emphasized. Here we use atomic force microscopy (AFM) to image a MUC2-rich mucus fraction isolated from pig jejunum. In the freshly isolated mucin fraction, we find direct evidence for trigonally linked structures, and their assembly into lamellar networks with a distribution of pore sizes from 20 to 200 nm. The networks are two-dimensional, with little interaction between lamellae. The existence of persistent cross-links between individual mucin polypeptides is consistent with a non-self-interacting lamellar model for intestinal mucus structure, rather than a physically entangled polymer network. We only observe collapsed entangled structures in purified mucin that has been stored in nonphysiological conditions.
- Published
- 2012
- Full Text
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42. Phase transitions and microstructure of emulsion systems prepared with acylglycerols/zinc stearate emulsifier.
- Author
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Macierzanka A, Szelag H, Moschakis T, and Murray BS
- Abstract
The emulsification processes, during which acylglycerols/zinc stearate emulsifier, water, and oil phase formed ternary systems, such as water-in-oil (W/O) emulsions, oil-in-water (O/W) dispersions, and unstable oil-water mixtures, were investigated in order to characterize the progressive transformations of the dispersed systems. The type, structure, and phase transitions of the systems were found to be determined by temperature and water phase content. Crystallization of the emulsifier caused the destabilization and subsequent phase inversion of the emulsions studied, at a temperature of 60-61 degrees C. The observed destabilization was temporary and led, at lower temperature, to W/O emulsions, "O/W + O" systems, or O/W dispersions, depending on the water content. Simultaneous emulsification and cooling of 20-50 wt % water systems resulted in the formation of stable W/O emulsions that contained a number of large water droplets with dispersed oil globules inside them ("W/O + O/W/O"). In water-rich systems (60-80 wt % of water), crystallization of the emulsifier was found to influence the formation of crystalline vesicle structures that coexisted, in the external water phase, with globules of crystallized oil phase. Results of calorimetric, rheological, and light scattering experiments, for the O/W dispersions obtained, indicate the possible transition of a monostearoylglycerol-based alpha-crystalline gel phase to a coagel state, in these multicomponent systems.
- Published
- 2006
- Full Text
- View/download PDF
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