1. Diversity of bat astroviruses in Lao PDR and Cambodia
- Author
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Philippe Dussart, Vibol Hul, Soubanh Silithammavong, Watthana Theppangna, Veasna Duong, Philippe Buchy, Kongsy Khammavong, Aneta Afelt, Roger Frutos, Zoe Greatorex, Sinpakone Singhalath, Sorn San, Keo Omaliss, Alexandre Hassanin, Audrey Lacroix, Lucy Keatts, Sokha Chea, Tracey Goldstein, Holl Davun, Damien O. Joly, Amanda E. Fine, Sarah H. Olson, Unité de Virologie / Virology Unit [Phnom Penh], Institut Pasteur du Cambodge, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Ministry of Agriculture, Forestry and Fisheries [Cambodia], Wildlife Conservation Society [Phnom Penh, Cambodia], Wildlife Conservation Society (WCS), Institut de Systématique, Evolution, Biodiversité (ISYEB ), Muséum national d'Histoire naturelle (MNHN)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Ministry of Agriculture and Forestry of Laos, Wildlife Conservation Society [Vientiane, Laos] (WCS), Metabiota Inc. [Vientiane, Laos], Metabiota Inc. [San Francisco], University of Warsaw (UW), University of California [Davis] (UC Davis), University of California, Metabiota Inc. [Nanaimo, British Columbia, Canada], Réseau International des Instituts Pasteur (RIIP), Interactions hôtes-vecteurs-parasites-environnement dans les maladies tropicales négligées dues aux trypanosomatides (UMR INTERTRYP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université de Bordeaux (UB), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), GlaxoSmithKline Vaccines [Singapore], GlaxoSmithKline [Headquarters, London, UK] (GSK), This study was made possible by the generous support of the American people through the United States Agency for International Development (USAID) Emerging Pandemic Threats PREDICT project (cooperative agreement number GHN-A-OO-09-00010-00)., Muséum national d'Histoire naturelle (MNHN)-Université Pierre et Marie Curie - Paris 6 (UPMC)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), and University of California (UC)
- Subjects
0301 basic medicine ,Phylogénie ,Range (biology) ,viruses ,L73 - Maladies des animaux ,Genetic diversity ,MESH: Mouth ,Hôte ,Feces ,fluids and secretions ,Génétique des populations ,MESH: Rectum ,MESH: Astroviridae Infections ,Astroviridae Infections ,Chiroptera ,Bats ,MESH: Animals ,MESH: Genetic Variation ,MESH: Phylogeny ,Lung ,Phylogeny ,Yinpterochiroptera ,biology ,Phylogenetic tree ,Astroviruses ,000 - Autres thèmes ,virus diseases ,MESH: Chiroptera ,MESH: Feces ,Infectious Diseases ,Yangochiroptera ,Laos ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,Biodiversité ,Cambodia ,MESH: Astroviridae ,Microbiology (medical) ,030106 microbiology ,Zoology ,Microbiology ,Article ,Astrovirus ,Virus des animaux ,03 medical and health sciences ,Variation génétique ,Lao PDR ,Phylogenetics ,Genetics ,Animals ,MESH: Lung ,Molecular Biology ,Ecology, Evolution, Behavior and Systematics ,Mouth ,MESH: Cambodia ,Rectum ,Genetic Variation ,biology.organism_classification ,Virology ,030104 developmental biology ,MESH: Laos ,Enquête pathologique ,Astroviridae ,Mammifère ,Rousettus - Abstract
Astroviruses are known to infect humans and a wide range of animal species, and can cause gastroenteritis in their hosts. Recent studies have reported astroviruses in bats in Europe and in several locations in China. We sampled 1876 bats from 17 genera at 45 sites from 14 and 13 provinces in Cambodia and Lao PDR respectively, and tested them for astroviruses. Our study revealed a high diversity of astroviruses among various Yangochiroptera and Yinpterochiroptera bats. Evidence for varying degrees of host restriction for astroviruses in bats was found. Furthermore, additional Pteropodid hosts were detected. The astroviruses formed distinct phylogenetic clusters within the genus Mamastrovirus, most closely related to other known bat astroviruses. The astrovirus sequences were found to be highly saturated indicating that phylogenetic relationships should be interpreted carefully. An astrovirus clustering in a group with other viruses from diverse hosts, including from ungulates and porcupines, was found in a Rousettus bat. These findings suggest that diverse astroviruses can be found in many species of mammals, including bats., Highlights • Diverse astroviruses detected in bats in Lao PDR and Cambodia • High polymorphism of astroviruses found in insectivorous and frugivorous bats • High level of genome saturation and hypermutation potential evidenced in astrovirus • Detection of additional astrovirus bat hosts, i.e. nectar bats from the genus Eonycteris • Discovery of a new species of astrovirus in fruit bats (Rousettus sp.), Graphical abstract. Image 1
- Published
- 2016
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