118 results on '"MEMOLI B"'
Search Results
2. Stop-flow technique for loco-regional delivery of high dose chemotherapy in the treatment of advanced pelvic cancers
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Strocchi, E, Iaffaioli, R.V, Facchini, G, Mantovani, G, Ricci, S, Cavallo, G, Tortoriello, A, D'Angelo, R, Formato, R, Rosato, G, Fiore, F, Iaccarino, V, Petrella, G, Memoli, B, Santangelo, M, and Camaggi, C.M
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- 2004
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3. HYPOXIC LOCOREGIONAL CHEMOTHERAPY (STOP-FLOW) IN ABDOMINAL AND PELVIC RELAPSES. PHASE I STUDY AND PHASE II PRELIMINARY DATA OF SITILO NATIONAL PROJECT.
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Facchini, G, Crovella, F, Tortoriello, A, Carbone, I, Turitto, G, Iaccarino, V, Memoli, B, Lombardi, R, Frattolillo, A, Mallarino, G, Coppola, Morelli G, Ghiani, M, Renda, A, Marzano, N, Memeo, V, Ionta, M T, Romano, G, Massidda, B, Mantovani, G, and Iaffaioli, R V
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- 2000
4. Inflammation may modulate interleukin 6 and C-reactive protein gene expression in the adipose tissue: the role of IL-6 cell membrane receptor
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MEMOLI B, PROCINO A, ESPOSITO P, GRANDALIANO G, PERTOSA G, DEL PRETE MA, ANDREUCCI M, DILILLO S, FERULANO G, CILLO C, SAVASTANO S, COLAO A, GUIDA B., CALABRO', Paolo, Memoli, B, Procino, A, Calabro', Paolo, Esposito, P, Grandaliano, G, Pertosa, G, DEL PRETE, Ma, Andreucci, M, Dilillo, S, Ferulano, G, Cillo, C, Savastano, S, Colao, A, and Guida, B.
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- 2007
5. Collaborative Study Group on SMC Membrane. Changes of serum albumin and C-reactive protein are related to changes of interleukin-6 release by peripheral blood mononuclear cells in hemodialysis patients treated with different membranes
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MEMOLI B, MINUTOLO R, BISESTI V, POSTIGLIONE, LOREDANA, CONTI A, MARZANO L, CAPUANO A, ANDREUCCI M, BALLETTA MM, TETTA C., GUIDA, BRUNA, Memoli, B, Minutolo, R, Bisesti, V, Postiglione, Loredana, Conti, A, Marzano, L, Capuano, A, Andreucci, M, Balletta, Mm, Guida, Bruna, and Tetta, C.
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- 2002
6. Angiotensin II antagonism restores impaired renal functional reserve in mild heart failure
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Rao MAE, Magri P, Cangianiello S, Russo R, Bellizzi V, Memoli B, Volpe M., DE NICOLA, Luca, Rao, Mae, Magri, P, Cangianiello, S, Russo, R, DE NICOLA, Luca, Bellizzi, V, Memoli, B, and Volpe, M.
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- 1998
7. Human plasma ultrafiltrate purification by membrane distillation: process optimisation and evaluation of its possible application on-line
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Criscuoli, A., Drioli, E., Capuano, A., Memoli, B., and Andreucci, V.E.
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- 2002
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8. Hepatocellular functions of human liver cells in oxygen-permeable membrane device
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De Bartolo L., Salerno S., Morelli S., Giorno L., Rende M., Memoli B., Andreucci, V.E., Bader, Drioli, Loredana De, Bartolo, Simona, Salerno, Sabrina, Morelli, Lidietta, Giorno, Maria, Rende, Memoli, Bruno, Vittorio Emanuele, Andreucci, Augustinus, Bader, and Enrico, Drioli
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Human liver ,Mechanical Engineering ,General Chemical Engineering ,chemistry.chemical_element ,General Chemistry ,Membrane bioreactor ,Oxygen ,hepatocellular functions ,chemistry ,Immunology ,Bioreactor ,Biophysics ,General Materials Science ,Semipermeable membrane ,oxygen-permeable membrane bioreactor ,Water Science and Technology - Abstract
In recent years numerous bioreactor hepatocytedevices using different materials and configura-tions have been developed and most attempts werefocused on adapting bioreactor technologies to theculture of primary hepatocytes. An oxygen perme-able membrane bioreactor based on the design byBader et al. [1–2] reconstructing the liver platearchitecture but with different configuration andutilizing human hepatocytes was assessed in thisstudy. In this device, human hepatocytes were cul-tured between flat-sheet gas-permeable polymericmembranes, which (1) ensure the diffusion of O
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- 2006
9. Evidence of Proximal Tubule Dysfunction in the Early Stages of Chronic Heart-Failure (HF)
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DE NICOLA, Luca, Rao MAE, Memoli B., DE NICOLA, Luca, Rao, Mae, and Memoli, B.
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- 1995
10. Membrane bioreactor using human hepatocytes adhered on Galactose-immobilized plasma processed membranes for the in vitro study of IL-6 effect
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De Bartolo L, Morelli S, Lopez LC, Giorno L, Salerno S, Rende M, Curcio E, Barbieri G, Procino A, Favia P, Memoli B, Andreucci VE, d'Agostino R, and Drioli E.
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- 2005
11. PES membranes modified by biomelecule immobilization for hepatocyte culture: effect of IL-6 on hepatocyte metabolism
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De Bartolo L, Morelli S, Lopez LC, Rende M, Salerno S, Procino A, Favia P, Memoli B, Andreucci VE, d'Agostino R, and Drioli
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- 2005
12. IL-6 production induced in monocytes by a serum factor from IgA nephropathy patients is inhibited in vitro by specific sugars
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RAMPINO T, LIBETTA C, MEMOLI B, DAL CANTON A., PALUMBO, GIUSEPPE, Rampino, T, Libetta, C, Palumbo, Giuseppe, Memoli, B, and DAL CANTON, A.
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- 1994
13. Effect of short-term synbiotic treatment on plasma p-cresol levels in patients with chronic renal failure: A randomized clinical trial.
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Guida, B, Germanò, R, Trio, R, Russo, D, Memoli, B, Grumetto, L, Barbato, F, and Cataldi, M
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- 2014
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14. Dietary phosphate restriction in dialysis patients: A new approach for the treatment of hyperphosphataemia.
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Guida, B., Piccoli, A., Trio, R., Laccetti, R., Nastasi, A., Paglione, A., Memoli, A., and Memoli, B.
- Abstract
Abstract: Background and Aim: Elevated serum phosphate and calcium–phosphate levels play an important role in the pathogenesis of vascular calcifications in uraemic patients and appear to be associated with increased cardiovascular mortality. We aimed to evaluate the effects of a partial replacement of food protein with a low-phosphorus and low-potassium whey protein concentrate on phosphate levels of dialysis patients with hyperphosphataemia. Methods and Results: Twenty-seven patients undergoing chronic haemodialysis were studied for a 3-month period. In the intervention group (n = 15), food protein were replaced by 30 or 40 g of low-phosphorus and low-potassium protein concentrate aimed at limiting the phosphate intake. In the control group (n = 12) no changes were made to their usual diet. Anthropometric measurements, biochemical markers and dietary interviews were registered at baseline and during the follow-up period. From baseline to the end of the study, in the intervention group, serum phosphate and circulating intact parathyroid hormone levels lessened significantly (8.3 ± 1.2 mg/dL vs 5.7 ± 1.4 mg/dL and 488 ± 205 pg/ml vs 177 ± 100 pg/ml respectively; p < 0.05) with decreasing of phosphate and potassium intake. No significant differences were found in the control group. No significant changes were observed in serum albumin, calcium, potassium, Kt/V, body weight and body composition in both the intervention and control groups. Conclusion: Dietary intake of phosphate mainly comes from protein sources, so dietary phosphorus restriction may lead to a protein/energy malnutrition in a dialysis patient. A phosphorus-controlled diet plan including a nutritional substitute resulted in serum phosphate and intact parathyroid hormone decrease without nutritional status modifications in dialysis patients. [Copyright &y& Elsevier]
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- 2011
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15. Serum fetuin a in hemodialysis: a link between derangement of calcium-phosphorus homeostasis and progression of atherosclerosis?
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Pertosa G, Simone S, Ciccone M, Porreca S, Zaza G, Dalfino G, Memoli B, Procino A, Bonomini M, Sirolli V, Castellano G, Gesualdo L, Ktena M, Schena FP, and Grandaliano G
- Abstract
BACKGROUND: Fetuin A, a circulating inhibitor of ectopic calcification, is downregulated in hemodialysis and has been shown to predict cardiovascular mortality in this setting. The association of altered calcium-phosphorus with serum fetuin A levels is still a matter of debate. Although carotid intima-media thickness (cIMT) is a strong predictor of major cardiovascular events, its association with serum fetuin A levels is poorly defined. STUDY DESIGN: Cohort study. PARTICIPANTS & SETTINGS: 174 uremic patients on long-term hemodialysis therapy enrolled in 4 university hospitals. PREDICTORS: Serum fetuin A levels at the beginning of the study (T0) and after 12 months (T12). OUTCOMES: Progression of atherosclerosis assessed by means of cIMT measurements at 24 months (T24); cardiovascular morbidity and mortality at 36 months. RESULTS: Serum fetuin A concentrations at T0 and T12 were 282.3 +/- 79.4 and 290.0 +/- 92.2 mug/mL, respectively. Mean T0 and T24 cIMT values were 1.02 +/- 0.2 and 1.06 +/- 0.2 mm, respectively (P < 0.001). Fatal and nonfatal cardiovascular disease occurred in 36 and 86 patients by 36 months, respectively. In multivariate logistic regression, higher calcium-phosphorus product was associated with lower serum fetuin A level (odds ratio, 0.96; 95% confidence interval [CI], 0.93 to 1.00; P = 0.02). Multiple regression analysis showed that T0 serum fetuin A level was associated with T24 cIMT (P = 0.01) after adjustments for age, cholesterol level, high-sensitivity C-reactive protein level, previous cardiovascular events, and T0 cIMT. In a multivariate Cox regression analysis, cardiovascular mortality was independently associated with a 1-tertile lower T0 serum fetuin A level, and a 1-tertile higher T0 cIMT value was independently associated with greater cardiovascular mortality (hazard ratio, 0.45; 95% CI, 0.15 to 0.65; P = 0.007 and hazard ratio, 10.00; 95% CI, 3.16 to 31.73; P < 0.001, respectively) after adjustment for age and previous cardiovascular events. LIMITATION: Length of follow-up. CONCLUSION: Calcium-phosphorus product in hemodialysis patients inversely correlated with serum fetuin A level, which, in turn, was associated inversely with progression of atherosclerotic lesions and cardiovascular mortality in this study population. Copyright © 2009 American Society for Nutrition [ABSTRACT FROM AUTHOR]
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- 2009
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16. Nutritional intervention in a hemodialysis pregnant woman: a case report
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GUIDA, B., POLLIO, F., NASTASI, A., TRIO, R., LACCETTI, R., DI LIETO, A., CITARELLA, F., and MEMOLI, B.
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- 2003
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17. Local activation of interleukin 6 signaling is associated with arteriovenous fistula stenosis in hemodialysis patients.
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Marrone D, Pertosa G, Simone S, Loverre A, Capobianco C, Cifarelli M, Memoli B, Schena FP, and Grandaliano G
- Abstract
BACKGROUND: Vascular access failure is the main cause of morbidity in hemodialysis patients. Stenosis of the arteriovenous fistula (AVF) is similar histologically to atherosclerosis. Recent studies showed that interleukin 6 (IL-6) has a key role in the pathogenesis of atherosclerosis by binding 2 specific receptors, gp80 and gp130. When activated, gp130 interacts with a tyrosine kinase, Janus kinase (JAK2), which then activates a transcription factor, signal transducers and activators of transcription (STAT3), directly turning on several proinflammatory genes. The aim of this study is to evaluate gp130 expression and JAK2/STAT3 activation within stenotic AVFs. METHODS: 44 patients undergoing surgery for AVF creation were enrolled; 10 of them had AVF failure with histologically proven AVF stenosis (wall-lumen ratio > 1). A venous fragment of the AVFs was collected during creation and revision of the vascular access. gp130 and gp80 expression, as well as JAK/STAT activation, were evaluated by means of confocal microscopy. Peripheral-blood mononuclear cells were isolated at the time of AVF creation and revision. RESULTS: gp130 protein expression, barely detectable in native AVFs, was strikingly increased within the venous branch of stenotic AVFs. The signaling subunit of the IL-6 receptor broadly colocalized with gp80, the IL-6-binding subunit. gp130-expressing cells were mainly CD34(+), suggesting that this receptor is expressed primarily by neovasculature endothelial cells. At the same time, a significant increase in phosphorylation of JAK2/STAT3 was observed in endothelial cells of stenotic AVFs. Interestingly, peripheral-blood mononuclear cells isolated at the time of AVF failure presented strikingly greater IL-6 expression compared with dialysis age-matched controls. CONCLUSION: IL-6 receptor activation may have a role in the pathogenesis of AVF failure in hemodialysis patients and may represent a potential therapeutic target in this setting.Copyright © 2007 by National Kidney Foundation, Inc. [ABSTRACT FROM AUTHOR]
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- 2007
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18. Cytokine Production in Standard Hemodialysis with a New Polyethersulfone Membrane: A Cross-Over Study.
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Memoli, B., Bisesti, V., Saravo, M.T., Esposito, P., D'Arcangelo, R., Nicosia, V., Clienti, C., Andreucci, M., Esposito, A., and Tetta, C.
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- 2003
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19. Atrial natriuretic factor in amniotic fluid and in maternal venous blood of pregnancies with fetal cardiac malformations and chromosomal abnormalities.
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di Lieto, A., Pollio, F., Catalano, D., Gallo, F., de Falco, M., Minutolo, R., and Memoli, B.
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- 2002
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20. Abnormalities of bioimpedance measures in overweight and obese hemodialyzed patients.
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Guida, B, De Nicola, L, Pecoraro, P, Trio, R, Di Paola, F, Iodice, C, Bellizzi, V, and Memoli, B
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HUMAN body composition ,OBESITY ,OVERWEIGHT persons - Abstract
BACKGROUND: The body composition in overweight and obese hemodialyzed patients (HD) remains ill-defined. This study evaluates in HD patients the influence of body size, as indicated by body mass index (BMI, kg/m²), on body composition by measuring bioimpedance analysis (BIA)-derived variables (phase angle (PA), fat-free mass (FFM) and body cell mass (BCM). METHODS: We studied 50 Caucasian patients (mean age 62.8±9.2 y) on standard bicarbonate hemodialysis for at least 12 months who regularly achieved dry weight in post-HD, received similar dialysis doses and were free from inflammation/infection. Thirty-eight gender- and age-matched healthy subjects were included as controls (CON). Both HD and CON were divided into three groups on the basis of their BMl(kg/m²) 18.5-24.9, normal-weight (NW); 25-29.9, overweight (OW); and ≥30, obese (OB). In HD patients, BIA was performed 30 min after the end of dialysis. RESULTS: Seven patients were obese (12%) while 16 were overweight (32%); in CON, 12 were obese (31%) and 12 overweight (31%). BIA-measured extracellular water was comparable in all groups. PA, which was similar in normal-weight HD and CON (6.2±0.9° and 6.3±0.8°), decreased in OW- and OB-HD patients (5.3±1.0° and 5.2±0.6°, respectively; P < 0.05 vs NW-HD) while it was unchanged in OW- and OB-CON (6.1±0.8° and 5.9±0.5°, P<0.05 vs respective HD groups). In OW and OB patients, the lower PA values were coupled with a major reduction of BIA-derived percentage BCM and FFM (P<0.05 vs NWHD, and vs OW- and OB-CON). In patients, PA and BCM correlated with anthropometry-measured FFM. Of note, serum albumin and protein catabolic rate were significantly reduced in OB patients. CONCLUSION: In overweight and obese HD patients, BIA-derived FFM, BCM and PA are significantly lower with respect to normal-weight patients and BMI-matched controls. These abnormalities of body composition are coupled... [ABSTRACT FROM AUTHOR]
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- 2001
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21. Comparison of vector and conventional bioelectrical impedance analysis in the optimal dry weight prescription in hemodialysis.
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Guida, Bruna, De Nicola, Luca, Trio, Rossella, Pecoraro, Pierluigi, Iodice, Carmela, Memoli, Bruno, Guida, B, De Nicola, L, Trio, R, Pecoraro, P, Iodice, C, and Memoli, B
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- 2000
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22. Cytokine Production in Haemodialysis.
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Memoli, B.
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- 1999
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23. Acute renal and immunological effects of low-dose cyclosporin in humans.
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de Nicola, L., Libetta, C., Memoli, B., Bellizzi, V., Colucci, G., Imperatore, P., Taccone, W., Oriente, L., Sepe, V., and Conte, G.
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- 1995
24. Interleukin-6 production induced in peripheral blood mononuclear cells by a serum factor from IgA nephropathy patients is inhibited in vitro by specific sugars.
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Rampino, T., Libetta, C., Palumbo, G., Memoli, B., and Canton, A. Dal
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The basal production of IL-6 by cultured peripheral blood mononuclear cells (PBMC) from patients with IgAN, is markedly higher (A, 109 pg/ml) as compared to that of PBMC of either patients without clinical signs (I, 39 pg/ml) or appropriate controls (C, 44 pg/ml). When PBMC from healthy subjects were incubated in the presence of sera from patients A, the IL-6 production was strongly enhanced. No such an effect was observed by stimulating PBMC with sera from the other two groups of subjects (I, C). In another experiment we observed that the IL-6 production stimulated by serum from patients A could be inhibited by addition of specific monosaccharides. The inhibitory effect was rapidly abolished when the sugar-containing medium was substituted with the original one. Finally molecular components from serum of A were grossly separated by gel column chromatography. Individual fractions were incubated with PBMC of C: fractions with Mr > 30 000 highly stimulated the release of IL-6 (up to 1320 pg/ml); fractions with lower molecular weight were inactive. The data suggest the presence of an IL-6 releasing factor in the serum of IgAN patients. Although the chemical nature of such a factor is not yet established, the observations reported focus our attention to the lectins family. Since this factor seems potentially important in the understanding of the pathogenesis of IgAN, both its isolation and structural/functional characterization deserve further efforts. [ABSTRACT FROM PUBLISHER]
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- 1994
25. Effects of uremia, acetate and bicarbonate dialysis on beta-adrenergic responsiveness as assessed by epinephrine-stimulated adenylate cyclase.
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Fuiano, Giorgio, Dal Canton, Antonio, Maj, Mario, Testa, Angelo, Memoli, Bruno, Conte, Giuseppe, Ariano, Maria Grazia, Guarriello, Luigi, Russo, Domenico, Andreucci, Vittorio E., Fuiano, G, Dal Canton, A, Maj, M, Testa, A, Memoli, B, Conte, G, Ariano, M G, Guarriello, L, Russo, D, and Andreucci, V E
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- 1989
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26. Influence of Concentration Polarization in Post-Dilutional Hernofiltration of Human Plasma.
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Calderaro, V., Memoli, B., Andreucci, V.E., Drioli, E., and Albanese, O.
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- 1980
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27. A New Semiautomated Apparatus for Hemofiltration.
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Andreucci, V. E., Calderaro, V., Memoli, B., and Terracciano, V.
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- 1979
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28. Human galactosylated membrane bioreactor for the long-term maintenance of liver specific functions
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De Bartolo, L., Morelli, S., Lopez, L.C., Giorno, L., Barbieri, G., Salerno, S., Rende, M., Curcio, E., Procino, A., Favia, P., Memoli, B., Andreucci, V.E., d'Agostino, R., and Drioli, E.
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- 2006
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29. J011: Abnormalities of renal functional reserve in patients with mild heart failure are corrected by losartan.
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Rao, M.A.E., Magri, P., Cangianiello, S., Russo, R., DeNicola, L., Bellizzi, V., Memoli, B., and Volpe, M.
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- 1998
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30. Abdominal and pelvic stop-flow (hypoxic, chemotherapeutic loco-regional treatment): Preliminary report of a phase I early phase II study
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G. Facchini, B. Massida, A. Tortoriello, M. Santangelo, F. Crovella, I. Carbone, G. Romano, E. D. Salvo, A. Novi, DODARO, CONCETTA ANNA, C. M. Camaggi, E. Strocchi, M. T. Ionta, V. Iaccarino, G. Mallarini, M. Ghiani, A. Bianchi, N. Marzano, V. Memeo, B. Memoli, G. C. Morelli, G. Mantovani, R. V. Iaffaioli, RENDA, ANDREA, Facchini, G, Massida, B, Tortoriello, A, Santangelo, Michele, Crovella, F, Carbone, I, Romano, G, Renda, A, DI SALVO, Enrico, Novi, A, Dodaro, CONCETTA ANNA, Camaggi, Cm, Strocchi, E, Ionta, Mt, Iaccarino, V, Mallarini, G, Ghiani, M, Bianchi, A, Marzano, N, Memeo, V, Memoli, B, Morelli, Gc, Mantovani, G, Iaffaioli, Rv, Facchini, G., Massida, B., Tortoriello, A., Santangelo, M., Crovella, F., Carbone, I., Romano, G., Renda, Andrea, Salvo, E. D., Novi, A., Camaggi, C. M., Strocchi, E., Ionta, M. T., Iaccarino, V., Mallarini, G., Ghiani, M., Bianchi, A., Marzano, N., Memeo, V., Memoli, B., Morelli, G. C., Mantovani, G., and Iaffaioli, R. V.
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- 1998
31. Extra-natriuretic effects of atrial peptide in humans
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Massimo Sabbatini, Giorgio Fuiano, G. Romano, Luca De Nicola, Giuseppe Conte, Alfredo Caglioti, Bruno Memoli, Bruno Cianciaruso, Domenico Russo, Antonio Dal Canton, DE NICOLA, L., Romano, G., Memoli, B., Cianciaruso, B., Sabbatini, Massimo, Russo, Domenico, Caglioti, A., Fuiano, G., DAL CANTON, A., Conte, G., De Nicola, L, Romano, G, Memoli, Bruno, Cianciaruso, B, Sabbatini, M, Russo, D, Caglioti, A, Fuiano, G, Dal Canton, A, DE NICOLA, Luca, Memoli, B, and Conte, Giuseppe
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Adult ,Male ,medicine.medical_specialty ,Potassium ,Natriuresis ,Renal function ,chemistry.chemical_element ,Blood Pressure ,Renin-Angiotensin System ,Excretion ,chemistry.chemical_compound ,Atrial natriuretic peptide ,Internal medicine ,medicine ,Homeostasis ,Humans ,Urea ,Salt intake ,Chemistry ,Diet, Sodium-Restricted ,Diuresis ,Endocrinology ,Nephrology ,Kaliuresis ,Atrial Natriuretic Factor ,Glomerular Filtration Rate - Abstract
Extra-natriuretic effects of atrial peptide in humans. To evaluate extra-natriuretic effects of atrial natriuretic peptide (ANP), plasma ANP (pANP) levels were assessed in seven healthy men on low-sodium diet (80mEq NaCl/day), in basal conditions and during stepwise infusion of human ANP (2, 4, 8 and 16 ng/min/kg). To determine the individual physiological (PHY) pANP level, we measured pANP in the same subjects after a high-salt diet (400 mEq NaCl/day), that is, in a physiological stimulation of ANP. We then compared the effects of the PHY levels of ANP to the effects of pharmacological (PHA) pANP levels. Neither PHY nor PHA pANP levels modified creatinine clearance or blood pressure. The progressive rise in pANP levels was associated with increases in urinary excretion of Na + , K + and urea. ANP alone respectively accounted for 41%, 30% and 92% of the increase in natriuresis, kaliuresis and urea excretion that occurred after changing salt intake from 80 to 400 mEq/day. Pharmacological ANP levels raised C H 2 O and reduced U Osm . Interestingly, PHA levels were associated with significant decrease in serum K + (from 4.5 ± .1 to 4.0 ± .1 mEq/liter) and plasma urea (from 31.9 ± 5 to 24.2 ± 4 mg/dl). The mean cumulative urinary potassium and urea losses corresponded to the theoretical body losses of potassium and urea; moreover, the individual cumulative urinary losses of potassium and urea significantly correlated with the corresponding decrement in their plasma levels. In conclusion, ANP has both physiological and pharmacological significance in the control of potassium and urea metabolism by decreasing plasma levels of K + and urea through effects on the renal excretory function.
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32. Plasma p-Cresol Lowering Effect of Sevelamer in Peritoneal Dialysis Patients: Evidence from a Cross-Sectional Observational Study
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Lucia Grumetto, Giuliana Salerno, Andrea Memoli, Francesco Barbato, Bruna Guida, Andrea Pota, Eleonora Riccio, Silvio Borrelli, Bruno Memoli, Gennaro Argentino, Mauro Cataldi, Guida, Bruna, Cataldi, Mauro, Riccio, Eleonora, Grumetto, Lucia, Pota, Andrea, Borrelli, S, Memoli, A, Barbato, Francesco, Argentino, Gennaro, Salerno, Giuliana, Memoli, Bruno, Guida, B, Cataldi, M, Riccio, E, Grumetto, L, Pota, A, Barbato, F, Argentino, G, Salerno, G, and Memoli, B.
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Mineral Metabolism and the Kidney ,Male ,medicine.medical_treatment ,lcsh:Medicine ,Toxicology ,Sevelamer ,Cresols ,Hyperphosphatemia ,Chronic Kidney Disease ,Blood plasma ,Toxin Binding ,Polyamines ,p-Cresol ,sevelamer ,lcsh:Science ,Chelating Agents ,Multidisciplinary ,end-stage renal disease ,Clinical Pharmacology ,uremic toxins ,Middle Aged ,peritoneal dialysi ,Nephrology ,Observational Studies ,Medicine ,Female ,Peritoneal Dialysis ,Research Article ,medicine.drug ,Adult ,Drugs and Devices ,medicine.medical_specialty ,lanthanum ,Clinical Research Design ,medicine.drug_class ,Urology ,Renal function ,Phosphates ,Peritoneal dialysis ,Internal medicine ,parasitic diseases ,medicine ,Humans ,Dialysis ,Aged ,Inflammation ,business.industry ,lcsh:R ,Immunity ,medicine.disease ,Phosphate binder ,Cross-Sectional Studies ,Endocrinology ,Kidney Failure, Chronic ,Clinical Immunology ,lcsh:Q ,business ,Kidney disease - Abstract
p-Cresol is a by-product of the metabolism of aromatic aminoacid operated by resident intestinal bacteria. In patients with chronic kidney disease, the accumulation of p-cresol and of its metabolite p-cresyl-sulphate, that represents more than 95% of circulating p-cresol, causes endothelial dysfunction and ultimately increases the cardiovascular risk of these patients. Therapeutic strategies able to reduce plasma p-cresol levels are highly demanded but unfortunately not available yet. Because it has been reported that the phosphate binder sevelamer also sequesters p-cresol in vitro we hypothesized that it could do so also in peritoneal dialysis patients. To explore this hypothesis we measured total cresol plasma concentrations in 57 patients with end-stage renal disease on peritoneal dialysis patients, 29 receiving sevelamer for the treatment of hyperphosphatemia and 28 patients not assuming this drug. Among the patients not assuming sevelamer, 16 were treated with lanthanum whereas the remaining 12 received no drug because they were not hyperphosphatemic. When we compared total p-cresol plasma concentrations in these different groups of patients, we, we found that plasma p-cresol levels were significantly lower in patients receiving sevelamer than in subjects receiving lanthanum or no drug. Patients assuming sevelamer had also lower high sensitivity C-reactive protein serum concentrations compared to patients not assuming this drug. Multiple linear regression analysis showed that Conversely, no difference either in residual glomerular filtration rate, total weekly dialysis dose or serum phosphate levels were observed among the different groups. These results suggest that sevelamer could be an effective strategy to lower p-cresol circulating levels in peritoneal dialysis patients in which it could also favorably affect the cardiovascular risk because of its anti-inflammatory effect.
- Published
- 2013
33. Downregulation of cell survival signalling pathways and increased cell damage in hydrogen peroxide-treated human renal proximal tubular cells by alpha-erythropoietin
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Bruno Memoli, Gaetano Lucisano, Pasquale Esposito, Michele Andreucci, Laura Fuiano, Pierangela Presta, Vincenzo Bisesti, F. Leone, Teresa Faga, Ashour Michael, Giorgio Fuiano, Domenico Russo, Andreucci, M., Fuiano, G., Presta, P., Lucisano, G., Leone, F., Fuiano, L., Bisesti, V., Esposito, P., Russo, Domenico, Memoli, B., Faga, T., Michael, A., Russo, D., and Memoli, Bruno
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medicine.medical_specialty ,Cell Survival ,Protein Kinase ,Biology ,Cell Line ,Kidney Tubules, Proximal ,Glycogen Synthase Kinase 3 ,Internal medicine ,medicine ,Humans ,Viability assay ,Phosphorylation ,Extracellular Signal-Regulated MAP Kinases ,Protein kinase B ,Cell damage ,Erythropoietin ,PI3K/AKT/mTOR pathway ,TOR Serine-Threonine Kinase ,Kinase ,Cell growth ,Extracellular Signal-Regulated MAP Kinase ,TOR Serine-Threonine Kinases ,Oxidative Stre ,Original Articles ,General Medicine ,Hydrogen Peroxide ,Cell Biology ,medicine.disease ,Oxidative Stress ,Endocrinology ,Cell culture ,Protein Kinases ,Proto-Oncogene Proteins c-akt ,medicine.drug ,Human ,Signal Transduction - Abstract
OBJECTIVE: Erythropoietin has been shown to have a protective effect in certain models of ischaemia-reperfusion, and in some cases the protection has been correlated with activation of signalling pathways known to play a role in cell survival and proliferation. We have studied whether erythropoietin would overcome direct toxic effects of hydrogen peroxide (H(2)O(2)) treatment to human renal proximal tubular (HK-2) cells. MATERIALS AND METHODS: HK-2 cells were incubated with H(2)O(2) (2 mm) for 2 h with or without erythropoietin at concentrations of 100 and 400 U/ml, and cell viability/proliferation was assessed by chemical reduction of MTT. Changes in phosphorylation state of the kinases Akt, glycogen synthase kinase-3beta (GSK-3beta), mammalian target of rapamycin (mTOR) and extracellular signal-regulated kinase 1 and 2 (ERK1/ERK2) were also analysed. RESULTS: Cells incubated with H(2)O(2) alone showed a significant decrease in viability, which did not significantly change by addition of erythropoietin at concentration of 100 U/ml, but was further reduced when concentration of erythropoietin was increased to 400 U/ml. Phosphorylation state of the kinases Akt, GSK-3beta, mTOR and ERK1/ERK2 of H(2)O(2)-treated HK-2 cells was slightly altered in the presence of erythropoietin at concentration of 100 U/ml, but was significantly less in the presence of erythropoietin at a concentration of 400 U/ml. Phosphorylation of forkhead transcription factor FKHRL1 was diminished in cells incubated with H(2)O(2) and erythropoietin at a concentration of 400 U/ml. CONCLUSIONS: Erythropoietin, at high concentrations, may significantly increase cellular damage in HK-2 cells subjected to oxidative stress, which may be due in part to decrease in activation of important signalling pathways involved in cell survival and/or cell proliferation.
- Published
- 2009
34. Stop-flow technique for loco-regional delivery of high dose chemotherapy in the treatment of advanced pelvic cancers
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B Memoli, F Fiore, G. Facchini, G Rosato, Michele Santangelo, A. Tortoriello, Rosario Vincenzo Iaffaioli, Giovanni Mantovani, R D'Angelo, G Petrella, E Strocchi, V. Iaccarino, R. Formato, S Ricci, G Cavallo, C.M. Camaggi, Strocchi E, Iaffaioli RV, Facchini G, Mantovani G, Ricci S, Cavallo G, Tortoriello A, D’Angelo R, Formato R, Rosato G, Fiore F, Iaccarino V, Petrella G, Memoli B, Santangelo M, Camaggi CM, Strocchi, E, Iaffaioli, Rv, Facchini, G, Mantovani, G, Ricci, S, Cavallo, G, Tortoriello, A, D'Angelo, R, Formato, R, Rosato, G, Fiore, F, Iaccarino, V, Petrella, G, Memoli, Bruno, Santangelo, M, Camaggi, Cm, E., Strocchi, Iaffaioli, R. V., G., Facchini, G., Mantovani, S., Ricci, G., Cavallo, A., Tortoriello, R., D'Angelo, R., Formato, G., Rosato, F., Fiore, V., Iaccarino, G., Petrella, B., Memoli, Santangelo, Michele, and C. M. C. a. m. a. g. g., I.
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Adult ,Male ,medicine.medical_specialty ,PHARMACOKINETICS ,Percutaneous ,DOXORUBICIN ,Adolescent ,Urology ,Hemodynamics ,Extracorporeal ,Bolus (medicine) ,Pharmacokinetics ,Humans ,Medicine ,Anthracyclines ,Aged ,Epirubicin ,Neoplasm Staging ,Pelvic Neoplasms ,REGIONAL PERFUSION ,COLORECTAL CANCER ,Antibiotics, Antineoplastic ,MITOMYCIN ,business.industry ,Mitomycin C ,General Medicine ,Middle Aged ,Treatment Outcome ,Oncology ,Chemotherapy, Cancer, Regional Perfusion ,Anesthesia ,Female ,Surgery ,Neoplasm Recurrence, Local ,Colorectal Neoplasms ,business ,Perfusion ,medicine.drug - Abstract
Aim. To verify the rationale of a pelvic stop-flow technique for the perfusion of high-doses of mitomycin C and anthacyclines in patients with inoperable, recurrent pelvic cancer. Methods. The stop-flow technique was realized by using percutaneous double-balloon arterial–venous catheters that selectively isolate the pelvic vascular section and a perfusion provided by an extracorporeal pump for 20 min. Ten patients (pts) with unresectable pelvic recurrence from colon-rectal cancer were treated with a combination of Mitomycin C (MMC, 20 mg/sqm) plus doxorubicin (DOXO, 75 mg/sqm; 8 pts) or epirubicin (EPI, 75 mg/sqm; 2 pts) infused into the isolated pelvic compartment. Blood samples were collected from the extracorporeal vascular flow and from peripheral plasma, and analysed for drug quantitation. Results. During the procedure, there were no technical or hemodynamic complications, and no deaths occurred during surgery or in the postoperative period. MMC and DOXO peak levels measured in the extracorporeal system which irrotates the tumor area, were on average 21.6 (range: 4.3–44.3, MMC) and 17.2 (range: 1.8–48.4, DOXO) times higher than those observed in the peripheral blood. Similarly; the area under concentration (AUC) versus time curves measured in the pelvic compartment during stop-flow perfusion were 19.9 (range: 3.8–45.0, MMC) and 13.4 (range: 1.2–26.6, DOXO) times higher than the corresponding value in peripheral circulation. The drug percentage eliminated in the ultra filtrate was only 7.7% (MMC) and 0.9% (DOXO), and the plasmatic AUC0–24 were similar to those observed with iv bolus of equivalent drug doses. Minimal systemic and local toxicities were observed. One complete pathological and 2 partial responses were observed; pain remission in 8/10 patients. Median survival was 12 months (8–31). Conclusion. The endo-arterial administration into the local vasculature produces high pelvic-systemic concentration gradients during the stop-flow perfusion with limited local and systemic toxicity. The encouraging clinical results suggest further evaluation.
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- 2004
35. Renal hemodynamic response to maximal vasodilating stimulus in healthy older subjects
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Alfredo Caglioti, Margherita Rosa, Giuseppe Conte, Luca De Nicola, Giuseppa Natale, Giorgio Fuiano, Giancarlo Gallo, Stale Sund, Giuseppe Mazza, Bruno Memoli, Michele Andreucci, Fuiano, G, Sund, S, Mazza, G, Rosa, M, Caglioti, A, Gallo, G, Natale, G, Andreucci, M, Memoli, Bruno, De Nicola, L, Conte, G., Memoli, B, DE NICOLA, Luca, and Conte, Giuseppe
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Adult ,Male ,medicine.medical_specialty ,Urinary system ,medicine.medical_treatment ,Vasodilator Agents ,Renal function ,Hemodynamics ,Kidney ,urologic and male genital diseases ,Renal Circulation ,Reference Values ,Internal medicine ,renal functional reserve ,medicine ,Humans ,Amino Acids ,Aged ,glomerular filtration rate ,Renal circulation ,arteriosclerosis ,business.industry ,aging ,blood pressure ,Arteriosclerosis ,medicine.disease ,Fibrosis ,Nephrectomy ,Drug Combinations ,medicine.anatomical_structure ,Endocrinology ,Kidney Tubules ,Nephrology ,Renal blood flow ,Cardiology ,Vascular Resistance ,Atrophy ,dopamine ,business - Abstract
Renal hemodynamic response to maximal vasodilating stimulus in healthy older subjects.BackgroundIt is still unclear whether age per se is associated with preservation of renal functional reserve, that is, of the increase in glomerular filtration rate (GFR) induced by appropriate vasodilating stimulus.MethodsTo gain insights into this issue, we evaluated the renal response to a maximal vasodilating stimulus, represented by the combined infusion of mixed amino acid solution (AA) and dopamine at renal dose (D), in 10 young subjects (median age of 30 years, range of 19 to 32) and in 11 subjects of older age (median age of 67 years, range of 65 to 76). Two further age-matched groups of young (N = 15) and older (N = 11) living kidney donors underwent renal needle biopsy immediately before nephrectomy to perform semiquantitative scoring (0 to 3) of arteriosclerosis in intrarenal arteries. All of the study subjects were nonsmokers with healthy status proven by extensive diagnostic evaluation excluding any risk factor of renal dysfunction.ResultsBasal renal plasma flow (RPF) and GFR were proportionally lower in older subjects (RPF, 361 ± 29 vs. 618 ± 34mL/min/1.73m2, P < 0.001; GFR, 79 ± 4 vs. 127 ± 5.8mL/min/1.73m2, P < 0.001). After AA + D, a significant increase of RPF and GFR was observed in both groups, but the older subjects exhibited a smaller percentage increment (RPF, 25.5 ± 4.8 vs. 42.4 ± 5.8, P < 0.05; GFR, 19.6 ± 5.7 vs. + 33.8 ± 6.4, P < 0.05). Furthermore, the maximal vasodilating stimulus was not able to restore renal hemodynamics in older subjects to the level measured in young controls at baseline. Renal vascular resistances were higher (P < 0.05) in the older subjects both at baseline (0.19 ± 0.02 vs. 0.09 ± 0.004mm Hg/mL/min) and after AA + D (0.14 ± 0.01 vs. 0.06 ± 0.004). Light microscopy examination detected the presence of a greater degree of arteriosclerosis at the level of interlobular and arcuate arteries (0.89 ± 0.15 vs. 0.45 ± 0.08) and interstitial fibrosis/tubular atrophy (1.18 ± 0.13 vs. 0.53 ± 0.13) in older than in young subjects.ConclusionsTherefore, aging has adverse effects on renal function despite the absence of any risk factor for renal disease, including chronic smoking: (1) GFR and RPF are lower, and (2) the renal response to maximal vasodilating stimulus is impaired. These aging-related alterations of renal hemodynamics are possibly due to organic lesions in renal vasculature.
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- 2001
36. Early impairment of renal hemodynamic reserve in patients with asymptomatic heart failure is restored by angiotensin II antagonism
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Rosaria Russo, Alessandro F. Mele, Sara Cangianiello, Bruno Memoli, Massimo Volpe, Michele Andreucci, Luca De Nicola, P. Magri, Maria A.E. Rao, V. Bellizzi, Magri, P, Rao, Ma, Cangianiello, S, Bellizzi, V, Russo, R, Mele, Af, Andreucci, M, Memoli, B, DE NICOLA, Luca, Volpe, M., Rao, Mae, Cangianiello, S., Bellizzi, V., Russo, R., Mele, A. F., Andreucci, M., Memoli, Bruno, and DE NICOLA, L.
- Subjects
Adult ,Male ,medicine.medical_specialty ,angiotensin ,heart failure ,hemodynamics ,kidney ,Renal function ,Angiotensin-Converting Enzyme Inhibitors ,urologic and male genital diseases ,Kidney ,Losartan ,Enalapril ,Physiology (medical) ,Internal medicine ,medicine ,Humans ,Amino Acids ,Heart Failure ,Renal sodium reabsorption ,business.industry ,Hemodynamics ,Middle Aged ,Angiotensin II ,Vasodilation ,Endocrinology ,medicine.anatomical_structure ,Regional Blood Flow ,Renal blood flow ,ACE inhibitor ,Chronic Disease ,Cardiology ,Female ,Cardiology and Cardiovascular Medicine ,business ,medicine.drug ,Glomerular Filtration Rate - Abstract
Background —The early/asymptomatic stages of heart failure (HF) are characterized by sodium retention secondary to derangement of sodium reabsorption at the proximal nephron level. Because this phenomenon is reversed by ACE inhibition, abnormalities of renal sodium handling may depend on intrarenal changes of angiotensin II (AII)/nitric oxide (NO) levels. Renal hemodynamic reserve (ie, the glomerular vasodilatory response to amino acid infusion) has been proposed as a reliable test to assess in vivo AII/NO balance. Methods and Results —In this study, the effects of 6 weeks of treatment with 5 mg/d of enalapril or with 50 mg/d of losartan on systemic hemodynamics and renal function were assessed, at baseline and after amino acid infusion (AA), in patients with mild HF (NYHA class I) and in healthy volunteers. Untreated HF patients showed a basal renal function comparable to that of healthy subjects. After AA, glomerular filtration rate and renal plasma flow significantly increased in healthy subjects (+29.0% and +30.4%, respectively), whereas no vasodilatory response was observed in HF. Although they did not affect basal renal hemodynamics, both enalapril and losartan restored a normal response to AA in HF patients. Blood pressure and heart rate were comparable in HF subjects and healthy subjects at baseline and were not modified by either treatment. Left ventricular ejection fraction was depressed in HF but did not change after either drug. Urinary excretions of cGMP and nitrate (indexes of NO activity in the kidney), comparable in healthy subjects and in HF patients, were unchanged by either enalapril or losartan and did not correlate with renal reserve. Conclusions —(1) Renal functional reserve is absent in patients with early/asymptomatic HF and normal renal function and (2) both enalapril and losartan restore a normal vasodilatory response to AA in these patients without affecting basal systemic and renal hemodynamics. These data suggest a major role of AII in the development of early abnormalities in patients with HF.
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- 1998
37. Inflammatory effects of peritoneal dialysis: evidence of systemic monocyte activation
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Luca De Nicola, Bruno Memoli, Teresa Rampino, Walter De Simone, Carmelo Libetta, Libetta, C, DE NICOLA, Luca, Rampino, T, DE SIMONE, W, Memoli, B., Libetta, C., DE NICOLA, L., Rampino, T., DE SIMONE, W., and Memoli, Bruno
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Adult ,Male ,medicine.medical_specialty ,medicine.medical_treatment ,Peritonitis ,Peripheral blood mononuclear cell ,Monocytes ,Peritoneal dialysis ,Peritoneal Dialysis, Continuous Ambulatory ,Renal Dialysis ,Internal medicine ,Humans ,Medicine ,Serum amyloid A ,Cells, Cultured ,Aged ,Uremia ,Serum Amyloid A Protein ,Interleukin-6 ,business.industry ,Beta-2 microglobulin ,Monocyte ,Continuous ambulatory peritoneal dialysis ,Acute-phase protein ,Middle Aged ,Endocrinology ,medicine.anatomical_structure ,Nephrology ,Immunology ,Kidney Failure, Chronic ,Regression Analysis ,Female ,Hemodialysis ,beta 2-Microglobulin ,business ,Biomarkers ,Acute-Phase Proteins - Abstract
Inflammatory effects of peritoneal dialysis: Evidence of systemic monocyte activation. We evaluated in peritonitis-free patients undergoing continuous ambulatory peritoneal dialysis (CAPD) the release of both interleukin-6 (IL-6) and β-2-microglobulin (β2m) by cultured peripheral blood mononuclear cells (PBMC), as well as the levels of serum amyloid A (SAA), that is, the main hepatic acute phase protein during inflammation. The same measurements were obtained in hemodialysis (HD) patients, uremic non-dialyzed patients (ESRD) and healthy controls (CON). In CAPD, IL-6 production from PBMC was markedly increased in comparison to the control value (600.7 ± 104.3 vs. 14.2 ± 3.6 pg/3 × 106 PBMC/24 hr, P < 0.005). Similarly, a striking enhancement of the PBMC release of β2m was detected in CAPD with respect to CON (10.1 ± 2.6 vs. 0.063 ± 0.013 µg/3 × 106 PBMC/24 hr, P < 0.001). Also, the SAA levels were significantly greater in CAPD patients (21.3 ± 8.7 µg/dl) than in controls (3.14 ± 0.17 µg/dl, P < 0.05). Analogous increases of both IL-6 and β2m cell releases, as well as of SAA levels, were observed in HD patients. No difference concerning the three parameters was detected between CON and ESRD. In conclusion, CAPD induces per se PBMC activation with an enhanced release of both IL-6 and β2m; this is associated to higher levels of SAA. These systemic inflammatory effects are comparable to those observed in HD patients indicating that CAPD is similar to HD in terms of biocompatibility of the treatment.
- Published
- 1996
38. Interleukin-2-induced renal dysfunction in cancer patients is reversed by low-dose dopamine infusion
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Bruno Memoli, Giovanna Palmieri, Paolo Romano, Giuseppe Conte, Giulia Pacchiano, Alessandro Morabito, Luca De Nicola, Antonella Scialò, Vittorio E. Andreucci, Carmelo Libetta, Rossella Lauria, Memoli, B, DE NICOLA, Luca, Libetta, C, Scialò, A, Pacchiano, G, Romano, P, Palmieri, G, Morabito, A, Lauria, R, Conte, Giuseppe, DE NICOLA, L, Scialo\', A, Conte, G, Andreucci, VITTORIO EMANUELE, Memoli, Bruno, DE NICOLA, L., Libetta, C., Scialo', A., Pacchiano, G., Romano, P., Palmieri, G., Morabito, A., Conte, G., and Andreucci, V. E.
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Oncotic pressure ,Male ,medicine.medical_specialty ,medicine.medical_treatment ,Dopamine ,Urology ,Renal function ,Dopamine agonist ,Internal medicine ,medicine ,Humans ,Infusions, Intravenous ,Aged ,Chemotherapy ,Analysis of Variance ,business.industry ,Sodium ,Cancer ,Blood Proteins ,Acute Kidney Injury ,Middle Aged ,medicine.disease ,Kidney Neoplasms ,Recombinant Proteins ,Discontinuation ,Endocrinology ,Nephrology ,Creatinine ,Interleukin-2 ,Female ,business ,Perfusion ,medicine.drug ,Glomerular Filtration Rate - Abstract
Recombinant interleukin-2 (rIL-2) is widely used in patients with advanced cancer to enhance killer cell functions. However, the main drawback of rIL-2 therapy is the frequent development of oliguric acute renal failure (ARF), presumably due to a vascular leak syndrome. The aim of this study was to evaluate the effect of low-dose dopamine infusion on this form of ARF. Nine patients with metastatic renal cancer and previous unilateral nephrectomy were treated with a continuous intravenous infusion of rIL-2 (3 × 10 6 Cetus units/m 2 /d) for 5 days (study A). After 1 week, all the patients repeated the same cycle, but with the addition of a continuous intravenous infusion of dopamine (2 μg/min/kg body weight) that was started at the third day of treatment (study B). During study A, all patients showed a progressive (up to 34%) decrease of creatinine clearance. After rIL-2 withdrawal, these alterations persisted and were associated with a reduction in urinary output, sodium urinary excretion, and plasma protein. In study B, dopamine administration after renal function impairment (‡glomerular filtration rate=−44%) led to a prompt improvement of creatinine clearance. Creatinine clearance showed a further significant enhancement after the withdrawal of both drugs, reaching a value within the baseline range on the third day of follow-up. Similarly, the decline in urinary output and sodium excretion during rIL-2 was promptly counteracted by dopamine; in addition, after withdrawal of rIL-2 and dopamine, plasma protein levels were normalized. In conclusion, our data suggest that rIL-2-induced ARF in cancer patients is due to renal hypoperfusion mainly caused by a reduction in oncotic pressure. Importantly, intravenous infusion of dopamine at low dosage is effective in reversing this form of renal impairment; such a therapeutic approach, while reducing recovery time, avoids dose reduction or discontinuation of rIL-2 treatment.
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- 1995
39. Loculated fluid collections evidenced by peritoneal scintigraphy.
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Argentino G, Riccio E, Celentano L, and Memoli B
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- Aged, Female, Glucans, Humans, Organotechnetium Compounds, Predictive Value of Tests, Radionuclide Imaging, Radiopharmaceuticals, Treatment Failure, Ascitic Fluid diagnostic imaging, Peritoneal Cavity diagnostic imaging, Peritoneal Dialysis adverse effects
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- 2014
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40. Phosphate levels in patients treated with low-flux haemodialysis, pre-dilution haemofiltration and haemodiafiltration: post hoc analysis of a multicentre, randomized and controlled trial.
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Locatelli F, Altieri P, Andrulli S, Sau G, Bolasco P, Pedrini LA, Basile C, David S, Gazzanelli L, Tampieri G, Isola E, Marzolla O, Memoli B, Ganadu M, Reina E, Bertoli S, Ferrara R, Casu D, Logias F, Tarchini R, Mattana G, Passaghe M, Fundoni G, Villa G, Di Iorio BR, Pontoriero G, and Zoccali C
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- Aged, Bicarbonates blood, Calcium blood, Female, Hemodiafiltration adverse effects, Hemofiltration, Humans, Kidney Failure, Chronic therapy, Male, Middle Aged, Parathyroid Hormone blood, Renal Dialysis, Kidney Failure, Chronic blood, Phosphates blood, Renal Replacement Therapy
- Abstract
Background: Whether convective therapies allow better control of serum phosphate (P) is still undefined, and no data are available concerning on-line haemofiltration (HF). The objectives of the study are to evaluate the effect of convective treatments (CTs) on P levels in comparison with low-flux haemodialysis (HD) and to evaluate the correlates of serum phosphate in a post hoc analysis of a randomized clinical trial., Methods: This analysis was performed in the database of a multicentre, open label and randomized controlled study in which 146 chronic HD patients from 27 Italian centres were randomly assigned to HD (70 patients) or CTs: on-line pre-dilution HF (36 patients) or on-line pre-dilution haemodiafiltration (40 patients)., Results: CTs did not affect P (P = 0.526), calcium (Ca) (P = 0.849) and parathyroid hormone levels (P = 0.622). P levels were associated with the use of phosphate binders including aluminium-based phosphate binders (P < 0.001) and sevelamer (P < 0.001), pre-dialysis bicarbonate levels (P < 0.001) and pre-dialysis blood K levels (P < 0.001). On multivariate analysis (generalized linear model), serum P was again largely unassociated with CTs (P = 0.631). Notably, participating centres were by far the strongest independent correlate of serum P, explaining 45.3% of the variance of serum P over the trial and this association was confirmed at multivariate analysis. Bicarbonate (P < 0.001) and, to a weaker extent, serum K (P = 0.032) were independently related to serum P., Conclusions: In comparison with low-flux HD, CTs did not significantly affect serum P levels. Participating centres were the main source of P variability during the trial followed by treatment with phosphate binders, serum bicarbonate and, to a weak extent, serum potassium levels (ClinicalTrials.gov Identifier: NCT011583309)., (© The Author 2014. Published by Oxford University Press on behalf of ERA-EDTA. All rights reserved.)
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- 2014
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41. Evidence that p-cresol and IL-6 are adsorbed by the HFR cartridge: towards a new strategy to decrease systemic inflammation in dialyzed patients?
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Riccio E, Cataldi M, Minco M, Argentino G, Russo R, Brancaccio S, Memoli A, Grumetto L, Postiglione L, Guida B, and Memoli B
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- Adsorption, Aged, Cresols blood, Female, Hemodialysis Solutions chemistry, Humans, Inflammation etiology, Inflammation prevention & control, Interleukin-6 blood, Kidney Failure, Chronic blood, Kidney Failure, Chronic complications, Kidney Failure, Chronic therapy, Male, Middle Aged, Serum Albumin chemistry, Cresols chemistry, Hemodiafiltration instrumentation, Hemodiafiltration methods, Interleukin-6 chemistry
- Abstract
Introduction: Hemodialysis (HD) and hemodiafiltration clear only with a low efficiency the plasma from interleukin-6 and p-cresol, two protein-bound uremic toxins associated with high cardiovascular risk in end stage renal disease. HFR Supra is a double-chamber hemodiafiltration system in which the ultrafiltrate returns to the patient after its regeneration through a resin cartridge that binds hydrophobic and protein-bound solutes. In the present study, we evaluated whether the HFR cartridge can also bind total p-cresol and IL-6 and remove them from the ultrafiltrate., Methods: We compared the levels of IL-6 and p-cresol in ultrafiltrate samples collected at the inlet (UFin) and at the outlet (UFout) of the cartridge at the start or at the end of a 240 min HFR session in 12 inflamed chronic HD patients. The pro-inflammatory activity of the ultrafiltrate samples was also determined by evaluating the changes that they induced in IL-6 mRNA expression and protein release in peripheral blood mononuclear cells from 12 healthy volunteers. IL-6 and p-cresol circulating levels were also assessed in peripheral plasma blood samples collected before and after HFR and, for comparison, a control HD., Results: p-Cresol and IL-6 were lower in UFout than in UFin both at the start and at the end of the HFR session, suggesting that they were retained by the cartridge. IL-6 mRNA expression and release were lower in PBMC incubated with UFout collected at the end than with UFin collected at the start of HFR, suggesting that passage through the cartridge reduced UF pro-inflammatory activity. Plasma total p-cresol decreased by about 53% after HFR, and 37% after HD. IL-6 circulating values were unmodified by either these dialysis procedures., Conclusions: This study shows that the HFR-Supra cartridge retains total p-cresol and IL-6 in the ultrafiltrate and lowers plasma total p cresol but not IL-6 levels., Trial Registration: ClinicalTrials.gov NCT01865773.
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- 2014
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42. Imaging of the peritoneum evaluated by 99mTc-icodextrin scintigraphy in peritoneal dialysis patients: preliminary data.
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Argentino G, Russo R, Maresca ID, Strazzullo T, Memoli A, Sodo M, Celentano L, and Memoli B
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- Adult, Aged, Animals, Extravasation of Diagnostic and Therapeutic Materials diagnostic imaging, Female, Hernia, Inguinal diagnostic imaging, Humans, Icodextrin, Kidney Failure, Chronic therapy, Male, Middle Aged, Peritoneum pathology, Radiography, Rats, Retroperitoneal Space diagnostic imaging, Thorax diagnostic imaging, Tissue Adhesions diagnostic imaging, Tissue Distribution, Dialysis Solutions pharmacokinetics, Glucans pharmacokinetics, Glucans toxicity, Glucose pharmacokinetics, Glucose toxicity, Organotechnetium Compounds pharmacokinetics, Organotechnetium Compounds toxicity, Peritoneal Cavity diagnostic imaging, Peritoneal Dialysis adverse effects, Peritoneum diagnostic imaging, Radiopharmaceuticals pharmacokinetics, Radiopharmaceuticals toxicity, Tomography, Emission-Computed, Single-Photon methods
- Abstract
Aim: In this study, we proposed a peritoneal scintigraphy with a different marker, the 99mTechnetium-Icodextrin, to evaluate the distribution of the dialysate within the peritoneal cavity in peritoneal dialysis (PD) patients., Methods: 99mTc-Icodextrin scintigraphy was performed in 16 PD patients. 0.5 ml of 7.5% Icodextrin solution was labeled with 74 megabecquerel (MBq) of 99mTc and then added to 2,000 ml of dialysate solution (2.5% dextrose). The peritoneum scintigraphy was performed by a SPECT gamma camera with the peritoneal cavity filled and after the complete drainage of the radio compound-dialysate mixture. The images were reviewed for evidence of peritoneal leaks, hernias, loculated fluid collections, and peritoneal membrane adhesions., Results: Abnormal findings were detected by 99mTc-Icodextrin scintigraphy in 4 (25%) out of 16 patients and included retroperitoneal (n = 1), diaphragmatic (n = 1) and inguinal (n = 1) leakages and peritoneal membrane adhesions (n = 1)., Conclusions: Peritoneum scintigraphy with 99mTc-Icodextrin is a useful method to detect some complications occurring during peritoneal dialysis; it offers excellent imaging to assess these complications., (© 2014 S. Karger AG, Basel.)
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- 2014
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43. Pleural effusion in peritoneal dialysis: overload or leakage?
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Riccio E, Argentino G, Pisani A, and Memoli B
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- Glucans adverse effects, Glucose adverse effects, Humans, Icodextrin, Organotechnetium Compounds, Pleural Effusion etiology, Radionuclide Imaging, Peritoneal Dialysis adverse effects, Pleural Effusion diagnostic imaging
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- 2013
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44. Hemodialysis does not affect ventricular-arterial coupling beyond the reduction of blood pressure and preload.
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Sasso L, Capuano A, Minco M, Paglia A, Pirozzi F, Memoli B, Carlomagno G, Petretta M, and Bonaduce D
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- Aged, Female, Humans, Kidney Failure, Chronic physiopathology, Kidney Failure, Chronic therapy, Male, Middle Aged, Atrial Function physiology, Blood Pressure physiology, Heart Rate physiology, Renal Dialysis adverse effects, Renal Dialysis trends, Ventricular Function physiology
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- 2013
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45. Plasma p-cresol lowering effect of sevelamer in peritoneal dialysis patients: evidence from a Cross-Sectional Observational Study.
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Guida B, Cataldi M, Riccio E, Grumetto L, Pota A, Borrelli S, Memoli A, Barbato F, Argentino G, Salerno G, and Memoli B
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- Adult, Aged, Cross-Sectional Studies, Female, Humans, Hyperphosphatemia blood, Hyperphosphatemia therapy, Male, Middle Aged, Phosphates blood, Sevelamer, Chelating Agents administration & dosage, Cresols blood, Kidney Failure, Chronic blood, Kidney Failure, Chronic therapy, Peritoneal Dialysis, Polyamines administration & dosage
- Abstract
p-Cresol is a by-product of the metabolism of aromatic aminoacid operated by resident intestinal bacteria. In patients with chronic kidney disease, the accumulation of p-cresol and of its metabolite p-cresyl-sulphate causes endothelial dysfunction and ultimately increases the cardiovascular risk of these patients. Therapeutic strategies to reduce plasma p-cresol levels are highly demanded but not available yet. Because it has been reported that the phosphate binder sevelamer sequesters p-cresol in vitro we hypothesized that it could do so also in peritoneal dialysis patients. To explore this hypothesis we measured total cresol plasma concentrations in 57 patients with end-stage renal disease on peritoneal dialysis, 29 receiving sevelamer for the treatment of hyperphosphatemia and 28 patients not assuming this drug. Among the patients not assuming sevelamer, 16 were treated with lanthanum whereas the remaining 12 received no drug because they were not hyperphosphatemic. Patients receiving sevelamer had plasma p-cresol and serum high sensitivity C-reactive protein concentrations significantly lower than those receiving lanthanum or no drug. Conversely, no difference was observed among the different groups either in residual glomerular filtration rate, total weekly dialysis dose, total clearance, urine volume, protein catabolic rate, serum albumin or serum phosphate levels. Multiple linear regression analysis showed that none of these variables predicted plasma p-cresol concentrations that, instead, negatively correlated with the use of sevelamer. These results suggest that sevelamer could be an effective strategy to lower p-cresol circulating levels in peritoneal dialysis patients in which it could also favorably affect cardiovascular risk because of its anti-inflammatory effect.
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- 2013
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46. Evidence on the prevalence and geographic distribution of major cardiovascular risk factors in Italy.
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Laccetti R, Pota A, Stranges S, Falconi C, Memoli B, Bardaro L, and Guida B
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- Adolescent, Adult, Aged, Aged, 80 and over, Cross-Sectional Studies, Female, Humans, Italy epidemiology, Male, Middle Aged, Prevalence, Residence Characteristics, Risk Factors, Smoking adverse effects, Young Adult, Cardiovascular Diseases etiology, Dyslipidemias epidemiology, Hypertension epidemiology, Overweight epidemiology, Smoking epidemiology
- Abstract
Objective: To assess the prevalence and geographic distribution of major cardiovascular risk factors in a large community-wide sample of the Italian population., Design: A cross-sectional survey. Standardized methods were used to collect and measure cardiovascular risk factors. Data were adjusted for survey weightings. Qualitative and quantitative variables were compared with parametric and non-parametric tests, as appropriate., Setting: Towns (n 193) across different Italian regions., Subjects: Unselected adults (n 24 213; 12 626 men; 11 587 women) aged 18-98 years (mean age 56·9 (sd 15·3) years), who volunteered to participate in a community-wide screening programme over a 2 d period in 2007., Results: Overall, the prevalence of major cardiovascular risk factors was: obesity, 22·7 % (women 18·9 %, men 26·1 %); overweight, 44·7 % (women 31·6 %, men 56·7 %); hypertension, 59·6 % (women 48·3 %, men 70·0 %); dyslipidaemia, 59·1 % (women 57·7 %, men 60·3 %); diabetes, 15·3 % (women 11·2 %, men 19·0 %) and smoking, 19·8 % (women 14·0 %, men 25·2 %). We found a high prevalence of unhealthy eating habits; fruit and vegetable consumption was below the recommended range in 60 % of the study population. Ninety per cent of the study population had more than one cardiovascular risk factor and 84 % had between two and five cardiovascular risk factors. There were differences among Italian macro-areas mainly for obesity, hypertension, dyslipidaemia and diabetes., Conclusions: The study provides alarming evidence on current prevalence data for major cardiovascular risk factors in a large sample of the Italian population. Particularly, obesity and hypertension represent a relevant public health problem. There is a pressing need for effective preventive health measures which must also take into account the differences among Italian macro-areas.
- Published
- 2013
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47. Immunomodulatory effect of continuous venovenous hemofiltration during sepsis: preliminary data.
- Author
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Servillo G, Vargas M, Pastore A, Procino A, Iannuzzi M, Capuano A, Memoli A, Riccio E, and Memoli B
- Subjects
- Adult, Aged, Aged, 80 and over, Female, Humans, Male, Middle Aged, Pilot Projects, Treatment Outcome, Acute Kidney Injury immunology, Acute Kidney Injury therapy, Hemofiltration methods, Immunomodulation immunology, Interleukin-6 immunology, Sepsis immunology, Sepsis therapy
- Abstract
Introduction: Severe sepsis and septic shock are the primary causes of multiple organ dysfunction syndrome (MODS), which is the most frequent cause of death in intensive care unit patients. Many pro- and anti-inflammatory mediators, such as interleukin-6 (IL-6), play a strategic role in septic syndrome. Continuous renal replacement therapy (CRRT) removes in a nonselective way pro- and anti-inflammatory mediators., Objective: To investigate the effects of continuous venovenous hemofiltration (CVVH) as an immunomodulatory treatment of sepsis in a prospective clinical study., Methods: High flux hemofiltration (Qf = 60 ml/Kg/hr) was performed for 72 hr in thirteen critically ill patients suffering from severe sepsis or septic shock with acute renal failure (ARF). IL-6 gene expression was measured by real-time PCR analysis on RNA extracted from peripheral blood mononuclear cell before beginning of treatment (T0) and after 12, 24, 48, and 72 hours (T1-4)., Results: Real-time PCR analysis demonstrated in twelve patients IL-6 mRNA reduction after 12 hours of treatment and a progressive increase after 24, 48, and 72 hours., Conclusions: We suggest that an immunomodulatory effect might exist during CVVH performed in critically ill patients with severe sepsis and septic shock. Our data show that the transcriptional activity of IL-6 increases during CVVH.
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- 2013
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48. Effects of a diet rich in N-3 polyunsaturated fatty acids on systemic inflammation in renal transplant recipients.
- Author
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Sabbatini M, Apicella L, Cataldi M, Maresca I, Nastasi A, Vitale S, Memoli B, Postiglione L, Riccio E, Gallo R, Federico S, and Guida B
- Subjects
- Adult, Biomarkers blood, C-Reactive Protein metabolism, Female, Humans, Immunosuppressive Agents therapeutic use, Inflammation drug therapy, Interleukin-6 blood, Lipids blood, Male, Middle Aged, Prognosis, Treatment Outcome, Diet, Fatty Acids, Unsaturated administration & dosage, Inflammation diet therapy, Kidney Transplantation
- Abstract
Objective: n-3 Polyunsaturated fatty acids (PUFAs) supplementation reduces systemic inflammation and improves renal and cardiovascular prognosis in kidney transplant recipients. However, patient compliance is poor because bad-tasting fish oils are used as an n-3 PUFA source. Therefore, we explored whether the beneficial effects of n-3 can be obtained by administering a diet based on n-3-rich foods., Methods: Sixty kidney transplant recipients were assigned to 2 different groups: the CON group (n = 28), which continued with their usual diet, and the DIET group (n = 32), which followed an n-3-rich diet for 6 months. Twenty-six patients in the DIET group and 24 in the CON group completed the study. End points of the study were changes in n-3 PUFAs intake, n-6:n-3 PUFAs ratio, systemic inflammation markers, and renal function during the 6 months of the dietary treatment., Results: Three and 6 months after the beginning of the study, n-3 PUFA intake was significantly higher and the n-6:n-3 PUFA ratio was markedly lower than baseline in the DIET group. Plasma total cholesterol, triglycerides, C-reactive protein, and interleukin (IL)-6 decreased as well. IL-6 mRNA levels in peripheral blood mononuclear cells were also lower than at the beginning of the study. Proteinuria and microalbuminuria were reduced by 50% with respect to the baseline, whereas glomerular filtration rate (GFR) was unchanged. No change in the aforementioned parameters was observed in the CON group throughout the study., Conclusion: In long-term kidney transplant recipients a naturally n-3 PUFA-rich dietary plan causes an increase in n-3 PUFA intake, decreases systemic inflammation and proteinuria, and improves plasma lipid pattern.
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- 2013
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49. Dietary intake as a link between obesity, systemic inflammation, and the assumption of multiple cardiovascular and antidiabetic drugs in renal transplant recipients.
- Author
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Guida B, Cataldi M, Maresca ID, Germanò R, Trio R, Nastasi AM, Federico S, Memoli A, Apicella L, Memoli B, and Sabbatini M
- Subjects
- Anthropometry, Body Composition, C-Reactive Protein metabolism, Cardiovascular Diseases blood, Demography, Energy Intake, Female, Humans, Inflammation blood, Interleukin-6 blood, Italy epidemiology, Male, Middle Aged, Cardiovascular Diseases epidemiology, Feeding Behavior, Hypoglycemic Agents therapeutic use, Inflammation epidemiology, Kidney Transplantation statistics & numerical data, Obesity epidemiology
- Abstract
We evaluated dietary intake and nutritional-inflammation status in ninety-six renal transplant recipients, 7.2 ± 5.0 years after transplantation. Patients were classified as normoweight (NW), overweight (OW), and obese (OB), if their body mass index was between 18.5 and 24.9, 25.0 and 29.9, and ≥ 30 kg/m(2), respectively. Food composition tables were used to estimate nutrient intakes. The values obtained were compared with those recommended in current nutritional guidelines. 52% of the patients were NW, 29% were OW, and 19% were OB. Total energy, fat, and dietary n-6 PUFAs intake was higher in OB than in NW. IL-6 and hs-CRP were higher in OB than in NW. The prevalence of multidrug regimen was higher in OB. In all patients, total energy, protein, saturated fatty acids, and sodium intake were higher than guideline recommendations. On the contrary, the intake of unsaturated and n-6 and n-3 polyunsaturated fatty acids and fiber was lower than recommended. In conclusion, the prevalence of obesity was high in our patients, and it was associated with inflammation and the assumption of multiple cardiovascular and antidiabetic drugs. Dietary intake did not meet nutritional recommendations in all patients, especially in obese ones, highlighting the need of a long-term nutritional support in renal transplant recipients.
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- 2013
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50. Human mature adipocytes express albumin and this expression is not regulated by inflammation.
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Sirico ML, Guida B, Procino A, Pota A, Sodo M, Grandaliano G, Simone S, Pertosa G, Riccio E, and Memoli B
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- Adult, Albumins genetics, Blotting, Western, C-Reactive Protein metabolism, Female, Humans, In Vitro Techniques, Inflammation physiopathology, Interleukin-6 metabolism, Male, Middle Aged, Nephelometry and Turbidimetry, Reverse Transcriptase Polymerase Chain Reaction, Adipocytes immunology, Adipocytes metabolism, Albumins metabolism, Inflammation metabolism
- Abstract
Aims: Our group investigated albumin gene expression in human adipocytes, its regulation by inflammation and the possible contribution of adipose tissue to albumin circulating levels., Methods: Both inflamed and healthy subjects provided adipose tissue samples. RT-PCR, Real-Time PCR, and Western Blot analysis on homogenates of adipocytes and pre-adipocytes were performed. In sixty-three healthy subjects and fifty-four micro-inflamed end stage renal disease (ESRD) patients circulating levels of albumin were measured by nephelometry; all subjects were also evaluated for body composition, calculated from bioelectrical measurements and an thropometric data., Results: A clear gene expression of albumin was showed in pre-adipocytes and, for the first time, in mature adipocytes. Albumin gene expression resulted significantly higher in pre-adipocytes than in adipocytes. No significant difference in albumin gene expression was showed between healthy controls and inflamed patients. A significant negative correlation was observed between albumin levels and fat mass in both healthy subjects and inflamed ESRD patients., Conclusions: In the present study we found first time evidence that human adipocytes express albumin. Our results also showed that systemic inflammation does not modulate albumin gene expression. The negative correlation between albumin and fat mass seems to exclude a significant contributing role of adipocyte in plasma albumin.
- Published
- 2012
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