1. The reaction of hydrogen atoms with methionine residues: A model of reductive radical stress causing tandem protein-lipid damage
- Author
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Sonia Manara, Maurizio Tamba, Immacolata Manco, Chryssostomos Chatgilialoglu, Armida Torreggiani, Carla Ferreri, M. Rosaria Faraone-Mennella, Ferreri, C, Manco, Immacolata, FARAONE MENNELLA, MARIA ROSARIA, Torreggiani, A, Tamba, M, Manara, S, and Chatgilialoglu, C.
- Subjects
liposomes ,Free Radicals ,RNase P ,Stereochemistry ,Radical ,Spectrum Analysis, Raman ,Solvated electron ,Models, Biological ,Biochemistry ,lipids ,chemistry.chemical_compound ,Isomerism ,Thioether ,Biomimetics ,Organic chemistry ,RNase A ,Molecular Biology ,chemistry.chemical_classification ,methionine ,Methionine ,Organic Chemistry ,Fatty acid ,reductive stress ,Ribonuclease, Pancreatic ,radicals ,chemistry ,Molecular Medicine ,Oxidation-Reduction ,Isomerization ,Cis–trans isomerism ,Hydrogen - Abstract
The occurrence of tandem damage, due to reductive radical stress involving proteins and lipids, is shown by using a biomimetic model. It is made of unsaturated lipid vesicle suspensions in phosphate buffer in the presence of methionine, either as a single amino acid or as part of a protein such as RNase A, which contains four methionine residues. The radical process starts with the formation of H(.) atoms by reaction of solvated electrons with dihydrogen phosphate anions, which selectively attack the thioether function of methionine. The modification of methionine to alpha-aminobutyric acid is accompanied by the formation of thiyl radicals, which in turn cause the isomerization of the cis fatty acid residues to the trans isomers. The relationship between methionine modification and lipid damage and some details of the reductive radical stress obtained by proteomic analysis of irradiated RNase A are presented.
- Published
- 2006