27 results on '"Louradour, J"'
Search Results
2. Transient Receptor Potential Vanilloid 4 channel participates in mouse ventricular electrical activity
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Chaigne, S., Cardouat, G., Louradour, J., Vaillant, F., Charron Guitoger, S., Sacher, F., Ducret, T., Guinamard, R., Vigmond, E., and Hof, T.
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- 2021
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3. Autonomous initiation of pacemaking by L-type Cav1.3 calcium channels in mouse sino-atrial myocytes
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Louradour, J., Bidaud, I., Le Guennec, J.Y., Mangoni, M., and Mesirca, P.
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- 2020
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4. Mitochondria and L-Type Ca2+ channels interplay in the regulation of Ca2+ dynamics in murine pacemaker cells
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Fossier, L., Torrente, A.G., Louradour, J., Bidaud, I., Mangoni, M., Mesirca, P., and Fauconnier, J.
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- 2019
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5. Transient Receptor Potential Vanilloid 4 channels participate in mouse ventricular action potentia
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Chaigne, S., Louradour, J., Charron, S., Brette, F., Ducret, T., and Hof, T.
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- 2019
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6. A COMPARISON BETWEEN SEQUENCE KERNELS FOR SVM SPEAKER VERIFICATION
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Daoudi, Kais, Louradour, J., Geometry and Statistics in acquisition data (GeoStat), Inria Bordeaux - Sud-Ouest, and Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)
- Subjects
[INFO.INFO-TS]Computer Science [cs]/Signal and Image Processing ,[SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
- Published
- 2009
7. Space Displacement Localization Neural Networks to locate origin points of handwritten text lines in historical documents.
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Moysset, Bastien, Adam, Pierre, Wolf, Christian, and Louradour, Jérôme
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- 2015
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8. Sample-Dependent Feature Selection for Faster Document Image Categorization.
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Louradour, J. and Kermorvant, C.
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- 2011
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9. Handwritten Mail Classification Experiments with the Rimes Database.
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Kermorvant, C. and Louradour, J.
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- 2010
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10. Curriculum learning.
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Bengio, Yoshua, Louradour, Jérôme, Collobert, Ronan, and Weston, Jason
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- 2009
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11. A comparison between sequence kernels for SVM speaker verification.
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Daoudi, K. and Louradour, J.
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- 2009
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12. State-of-the-art sequence kernels for SVM speaker verification.
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Louradour, J. and Daoudi, K.
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- 2008
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13. A novel strategy for speaker verification based on SVM classification of pairs of speech sequences.
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Daoudi, K. and Louradour, J.
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- 2007
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14. SVM Speaker Verification using an Incomplete Cholesky Decomposition Sequence Kernel.
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Louradour, J., Daoudi, K., and Bach, F.
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- 2006
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15. Audio Events Detection in Public Transport Vehicle.
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Rouas, J.-L., Louradour, J., and Ambellouis, S.
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- 2006
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16. Discriminative Power of Transient Frames in Speaker Recognition.
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Louradour, J., Daoudi, K., Andre-Obrecht, R., and Sabatier, P.
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- 2005
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17. Gene- and variant-specific efficacy of serum/glucocorticoid-regulated kinase 1 inhibition in long QT syndrome types 1 and 2
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Giannetti, Federica, Barbieri, Miriam, Shiti, Assad, Casini, Simona, Sager, Philip T, Das, Saumya, Pradhananga, Sabindra, Srinivasan, Dinesh, Nimani, Saranda, Alerni, Nicolò, Louradour, Julien, Mura, Manuela, Gnecchi, Massimiliano, Brink, Paul, Zehender, Manfred, Koren, Gideon, Zaza, Antonio, Crotti, Lia, Wilde, Arthur A M, Schwartz, Peter J, Remme, Carol Ann, Gepstein, Lior, Sala, Luca, Odening, Katja E, Giannetti, F, Barbieri, M, Shiti, A, Casini, S, Sager, P, Das, S, Pradhananga, S, Srinivasan, D, Nimani, S, Alerni, N, Louradour, J, Mura, M, Gnecchi, M, Brink, P, Zehender, M, Koren, G, Zaza, A, Crotti, L, Wilde, A, Schwartz, P, Remme, C, Gepstein, L, Sala, L, and Odening, K
- Subjects
Cellular electrophysiology ,Physiology (medical) ,LQTS ,Animal model ,610 Medicine & health ,610 Medizin und Gesundheit ,Cardiology and Cardiovascular Medicine ,Genotype-specific therapy ,hiPSC ,Mechanism-based therapy - Abstract
Aims Current long QT syndrome (LQTS) therapy, largely based on beta-blockade, does not prevent arrhythmias in all patients; therefore, novel therapies are warranted. Pharmacological inhibition of the serum/glucocorticoid-regulated kinase 1 (SGK1-Inh) has been shown to shorten action potential duration (APD) in LQTS type 3. We aimed to investigate whether SGK1-Inh could similarly shorten APD in LQTS types 1 and 2. Methods and results Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) and hiPSC-cardiac cell sheets (CCS) were obtained from LQT1 and LQT2 patients; CMs were isolated from transgenic LQT1, LQT2, and wild-type (WT) rabbits. Serum/glucocorticoid-regulated kinase 1 inhibition effects (300 nM–10 µM) on field potential durations (FPD) were investigated in hiPSC-CMs with multielectrode arrays; optical mapping was performed in LQT2 CCS. Whole-cell and perforated patch clamp recordings were performed in isolated LQT1, LQT2, and WT rabbit CMs to investigate SGK1-Inh (3 µM) effects on APD. In all LQT2 models across different species (hiPSC-CMs, hiPSC-CCS, and rabbit CMs) and independent of the disease-causing variant (KCNH2-p.A561V/p.A614V/p.G628S/IVS9-28A/G), SGK1-Inh dose-dependently shortened FPD/APD at 0.3–10 µM (by 20–32%/25–30%/44–45%). Importantly, in LQT2 rabbit CMs, 3 µM SGK1-Inh normalized APD to its WT value. A significant FPD shortening was observed in KCNQ1-p.R594Q hiPSC-CMs at 1/3/10 µM (by 19/26/35%) and in KCNQ1-p.A341V hiPSC-CMs at 10 µM (by 29%). No SGK1-Inh-induced FPD/APD shortening effect was observed in LQT1 KCNQ1-p.A341V hiPSC-CMs or KCNQ1-p.Y315S rabbit CMs at 0.3–3 µM. Conclusion A robust SGK1-Inh-induced APD shortening was observed across different LQT2 models, species, and genetic variants but less consistently in LQT1 models. This suggests a genotype- and variant-specific beneficial effect of this novel therapeutic approach in LQTS.
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- 2023
18. Computational modelling of mouse atrio ventricular node action potential and automaticity.
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Bartolucci C, Mesirca P, Ricci E, Sales-Bellés C, Torre E, Louradour J, Mangoni ME, and Severi S
- Abstract
The atrioventricular node (AVN) is a crucial component of the cardiac conduction system. Despite its pivotal role in regulating the transmission of electrical signals between atria and ventricles, a comprehensive understanding of the cellular electrophysiological mechanisms governing AVN function has remained elusive. This paper presents a detailed computational model of mouse AVN cell action potential (AP). Our model builds upon previous work and introduces several key refinements, including accurate representation of membrane currents and exchangers, calcium handling, cellular compartmentalization, dynamic update of intracellular ion concentrations, and calcium buffering. We recalibrated and validated the model against existing and unpublished experimental data. In control conditions, our model reproduces the AVN AP experimental features, (e.g. rate = 175 bpm, experimental range [121, 191] bpm). Notably, our study sheds light on the contribution of L-type calcium currents, through both Ca
v 1.2 and Cav 1.3 channels, in AVN cells. The model replicates several experimental observations, including the cessation of firing upon block of Cav 1.3 or INa,r current. If block induces a reduction in beating rate of 11%. In summary, this work presents a comprehensive computational model of mouse AVN cell AP, offering a valuable tool for investigating pacemaking mechanisms and simulating the impact of ionic current blockades. By integrating calcium handling and refining formulation of ionic currents, our model advances understanding of this critical component of the cardiac conduction system, providing a platform for future developments in cardiac electrophysiology. KEY POINTS: This paper introduces a comprehensive computational model of mouse atrioventricular node (AVN) cell action potentials (APs). Our model is based on the electrophysiological data from isolated mouse AVN cells and exhibits an action potential and calcium transient that closely match the experimental records. By simulating the effects of blocking specific ionic currents, the model effectively predicts the roles of L-type Cav 1.2 and Cav 1.3 channels, T-type calcium channels, sodium currents (TTX-sensitive and TTX-resistant), and the funny current (If ) in AVN pacemaking. The study also emphasizes the significance of other ionic currents, including IKr , Ito , IKur , in regulating AP characteristics and cycle length in AVN cells. The model faithfully reproduces the rate dependence of action potentials under pacing, opening the possibility of use in impulse propagation models. The population-of-models approach showed the robustness of this new AP model in simulating a wide spectrum of cellular pacemaking in AVN., (© 2024 The Author(s). The Journal of Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society.)- Published
- 2024
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19. KCNQ1 suppression-replacement gene therapy in transgenic rabbits with type 1 long QT syndrome.
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Bains S, Giammarino L, Nimani S, Alerni N, Tester DJ, Kim CSJ, Christoforou N, Louradour J, Horváth A, Beslac O, Barbieri M, Matas L, Hof TS, Lopez R, Perez-Feliz S, Parodi C, Garcia Casalta LG, Jurgensen J, Barry MA, Bego M, Keyes L, Owens J, Pinkstaff J, Koren G, Zehender M, Brunner M, Casoni D, Praz F, Haeberlin A, Brooks G, Ackerman MJ, and Odening KE
- Abstract
Background and Aims: Type 1 long QT syndrome (LQT1) is caused by pathogenic variants in the KCNQ1-encoded Kv7.1 potassium channels, which pathologically prolong ventricular action potential duration (APD). Herein, the pathologic phenotype in transgenic LQT1 rabbits is rescued using a novel KCNQ1 suppression-replacement (SupRep) gene therapy., Methods: KCNQ1-SupRep gene therapy was developed by combining into a single construct a KCNQ1 shRNA (suppression) and an shRNA-immune KCNQ1 cDNA (replacement), packaged into adeno-associated virus serotype 9, and delivered in vivo via an intra-aortic root injection (1E10 vg/kg). To ascertain the efficacy of SupRep, 12-lead electrocardiograms were assessed in adult LQT1 and wild-type (WT) rabbits and patch-clamp experiments were performed on isolated ventricular cardiomyocytes., Results: KCNQ1-SupRep treatment of LQT1 rabbits resulted in significant shortening of the pathologically prolonged QT index (QTi) towards WT levels. Ventricular cardiomyocytes isolated from treated LQT1 rabbits demonstrated pronounced shortening of APD compared to LQT1 controls, leading to levels similar to WT (LQT1-UT vs. LQT1-SupRep, P < .0001, LQT1-SupRep vs. WT, P = ns). Under β-adrenergic stimulation with isoproterenol, SupRep-treated rabbits demonstrated a WT-like physiological QTi and APD90 behaviour., Conclusions: This study provides the first animal-model, proof-of-concept gene therapy for correction of LQT1. In LQT1 rabbits, treatment with KCNQ1-SupRep gene therapy normalized the clinical QTi and cellular APD90 to near WT levels both at baseline and after isoproterenol. If similar QT/APD correction can be achieved with intravenous administration of KCNQ1-SupRep gene therapy in LQT1 rabbits, these encouraging data should compel continued development of this gene therapy for patients with LQT1., (© The Author(s) 2024. Published by Oxford University Press on behalf of the European Society of Cardiology.)
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- 2024
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20. Activation of hTREK-1 by polyunsaturated fatty acids involves direct interaction.
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Bechard E, Arel E, Bride J, Louradour J, Bussy X, Elloumi A, Vigor C, Soule P, Oger C, Galano JM, Durand T, Le Guennec JY, Moha-Ou-Maati H, and Demion M
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- Humans, HEK293 Cells, Patch-Clamp Techniques, Membrane Fluidity drug effects, Potassium Channels, Tandem Pore Domain metabolism, Fatty Acids, Unsaturated metabolism, Fatty Acids, Unsaturated pharmacology
- Abstract
TREK-1 is a mechanosensitive channel activated by polyunsaturated fatty acids (PUFAs). Its activation is supposed to be linked to changes in membrane tension following PUFAs insertion. Here, we compared the effect of 11 fatty acids and ML402 on TREK-1 channel activation using the whole cell and the inside-out configurations of the patch-clamp technique. Firstly, TREK-1 activation by PUFAs is variable and related to the variable constitutive activity of TREK-1. We observed no correlation between TREK-1 activation and acyl chain length or number of double bonds suggesting that the bilayer-couple hypothesis cannot explain by itself the activation of TREK-1 by PUFAs. The membrane fluidity measurement is not modified by PUFAs at 10 µM. The spectral shift analysis in TREK-1-enriched microsomes indicates a K
D,TREK1 at 44 µM of C22:6 n-3. PUFAs display the same activation and reversible kinetics than the direct activator ML402 and activate TREK-1 in both whole-cell and inside-out configurations of patch-clamp suggesting that the binding site of PUFAs is accessible from both sides of the membrane, as for ML402. Finally, we proposed a two steps mechanism: first, insertion into the membrane, with no fluidity or curvature modifications at 10 µM, and then interaction with TREK-1 channel to open it., (© 2024. The Author(s).)- Published
- 2024
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21. Simultaneous assessment of mechanical and electrical function in Langendorff-perfused ex-vivo mouse hearts.
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Louradour J, Ottersberg R, Segiser A, Olejnik A, Martínez-Salazar B, Siegrist M, Egle M, Barbieri M, Nimani S, Alerni N, Döring Y, Odening KE, and Longnus S
- Abstract
Background: The Langendorff-perfused ex-vivo isolated heart model has been extensively used to study cardiac function for many years. However, electrical and mechanical function are often studied separately-despite growing proof of a complex electro-mechanical interaction in cardiac physiology and pathology. Therefore, we developed an isolated mouse heart perfusion system that allows simultaneous recording of electrical and mechanical function., Methods: Isolated mouse hearts were mounted on a Langendorff setup and electrical function was assessed via a pseudo-ECG and an octapolar catheter inserted in the right atrium and ventricle. Mechanical function was simultaneously assessed via a balloon inserted into the left ventricle coupled with pressure determination. Hearts were then submitted to an ischemia-reperfusion protocol., Results: At baseline, heart rate, PR and QT intervals, intra-atrial and intra-ventricular conduction times, as well as ventricular effective refractory period, could be measured as parameters of cardiac electrical function. Left ventricular developed pressure (DP), left ventricular work (DP-heart rate product) and maximal velocities of contraction and relaxation were used to assess cardiac mechanical function. Cardiac arrhythmias were observed with episodes of bigeminy during which DP was significantly increased compared to that of sinus rhythm episodes. In addition, the extrasystole-triggered contraction was only 50% of that of sinus rhythm, recapitulating the "pulse deficit" phenomenon observed in bigeminy patients. After ischemia, the mechanical function significantly decreased and slowly recovered during reperfusion while most of the electrical parameters remained unchanged. Finally, the same electro-mechanical interaction during episodes of bigeminy at baseline was observed during reperfusion., Conclusion: Our modified Langendorff setup allows simultaneous recording of electrical and mechanical function on a beat-to-beat scale and can be used to study electro-mechanical interaction in isolated mouse hearts., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (© 2023 Louradour, Ottersberg, Segiser, Olejnik, Martínez-Salazar, Siegrist, Egle, Barbieri, Nimani, Alerni, Döring, Odening and Longnus.)
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- 2023
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22. Injectable contraceptive Depo-Provera induces erratic beating patterns in patient-specific induced pluripotent stem cell-derived cardiomyocytes with long QT syndrome type 2.
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Pinsky AM, Gao X, Bains S, Kim CJ, Louradour J, Odening KE, Tester DJ, Giudicessi JR, and Ackerman MJ
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- Humans, Female, Adult, Medroxyprogesterone Acetate pharmacology, Progestins, Myocytes, Cardiac, Contraceptives, Oral, Arrhythmias, Cardiac, Induced Pluripotent Stem Cells, Long QT Syndrome genetics
- Abstract
Background: Long QT syndrome type 2 (LQT2) is caused by pathogenic variants in KCNH2. LQT2 may manifest as QT prolongation on an electrocardiogram and present with arrhythmic syncope/seizures and sudden cardiac arrest/death. Progestin-based oral contraceptives may increase the risk of LQT2-triggered cardiac events in women. We previously reported on a woman with LQT2 and recurrent cardiac events temporally related and attributed to the progestin-based contraceptive medroxyprogesterone acetate ("Depo-Provera" [Depo] MilliporeSigma, Catalog# 1378001, St. Louis, MO)., Objective: The purpose of this study was to evaluate the arrhythmic risk of Depo in a patient-specific induced pluripotent stem cell-derived cardiomyocyte (iPSC-CM) model of LQT2., Methods: An iPSC-CM line was generated from a 40-year-old woman with p.G1006Afs∗49-KCNH2. A CRISPR/Cas9 gene-edited/variant-corrected isogenic control iPSC-CM line was generated. FluoVolt (Invitrogen, F10488, Waltham, MA) was used to measure the action potential duration after treatment with 10 μM Depo. Erratic beating patterns characterized as alternating spike amplitudes, alternans, or early afterdepolarization-like phenomena were assessed using multielectrode array (MEA) after 10 μM Depo, 1 μM isoproterenol (ISO), or combined Depo + ISO treatment., Results: Depo treatment shortened the action potential duration at 90% repolarization of G1006Afs∗49 iPSC-CMs from 394 ± 10 to 303 ± 10 ms (P < .0001). Combined Depo + ISO treatment increased the percentage of electrodes displaying erratic beating in G1006Afs∗49 iPSC-CMs (baseline: 18% ± 5% vs Depo + ISO: 54% ± 5%; P < .0001) but not in isogenic control iPSC-CMs (baseline: 0% ± 0% vs Depo + ISO: 10% ± 3%; P = .9659)., Conclusion: This cell study provides a potential mechanism for the patient's clinically documented Depo-associated episodes of recurrent ventricular fibrillation. This in vitro data should prompt a large-scale clinical assessment of Depo's potential proarrhythmic effect in women with LQT2., (Copyright © 2023 Heart Rhythm Society. Published by Elsevier Inc. All rights reserved.)
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- 2023
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23. Gene- and variant-specific efficacy of serum/glucocorticoid-regulated kinase 1 inhibition in long QT syndrome types 1 and 2.
- Author
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Giannetti F, Barbieri M, Shiti A, Casini S, Sager PT, Das S, Pradhananga S, Srinivasan D, Nimani S, Alerni N, Louradour J, Mura M, Gnecchi M, Brink P, Zehender M, Koren G, Zaza A, Crotti L, Wilde AAM, Schwartz PJ, Remme CA, Gepstein L, Sala L, and Odening KE
- Subjects
- Animals, Humans, Rabbits, Glucocorticoids, KCNQ1 Potassium Channel genetics, Arrhythmias, Cardiac genetics, Myocytes, Cardiac physiology, Action Potentials physiology, Long QT Syndrome drug therapy, Long QT Syndrome genetics, Induced Pluripotent Stem Cells
- Abstract
Aims: Current long QT syndrome (LQTS) therapy, largely based on beta-blockade, does not prevent arrhythmias in all patients; therefore, novel therapies are warranted. Pharmacological inhibition of the serum/glucocorticoid-regulated kinase 1 (SGK1-Inh) has been shown to shorten action potential duration (APD) in LQTS type 3. We aimed to investigate whether SGK1-Inh could similarly shorten APD in LQTS types 1 and 2., Methods and Results: Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) and hiPSC-cardiac cell sheets (CCS) were obtained from LQT1 and LQT2 patients; CMs were isolated from transgenic LQT1, LQT2, and wild-type (WT) rabbits. Serum/glucocorticoid-regulated kinase 1 inhibition effects (300 nM-10 µM) on field potential durations (FPD) were investigated in hiPSC-CMs with multielectrode arrays; optical mapping was performed in LQT2 CCS. Whole-cell and perforated patch clamp recordings were performed in isolated LQT1, LQT2, and WT rabbit CMs to investigate SGK1-Inh (3 µM) effects on APD. In all LQT2 models across different species (hiPSC-CMs, hiPSC-CCS, and rabbit CMs) and independent of the disease-causing variant (KCNH2-p.A561V/p.A614V/p.G628S/IVS9-28A/G), SGK1-Inh dose-dependently shortened FPD/APD at 0.3-10 µM (by 20-32%/25-30%/44-45%). Importantly, in LQT2 rabbit CMs, 3 µM SGK1-Inh normalized APD to its WT value. A significant FPD shortening was observed in KCNQ1-p.R594Q hiPSC-CMs at 1/3/10 µM (by 19/26/35%) and in KCNQ1-p.A341V hiPSC-CMs at 10 µM (by 29%). No SGK1-Inh-induced FPD/APD shortening effect was observed in LQT1 KCNQ1-p.A341V hiPSC-CMs or KCNQ1-p.Y315S rabbit CMs at 0.3-3 µM., Conclusion: A robust SGK1-Inh-induced APD shortening was observed across different LQT2 models, species, and genetic variants but less consistently in LQT1 models. This suggests a genotype- and variant-specific beneficial effect of this novel therapeutic approach in LQTS., Competing Interests: Conflict of interest: None declared., (© The Author(s) 2023. Published by Oxford University Press on behalf of the European Society of Cardiology.)
- Published
- 2023
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24. L-Type Ca v 1.3 Calcium Channels Are Required for Beta-Adrenergic Triggered Automaticity in Dormant Mouse Sinoatrial Pacemaker Cells.
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Louradour J, Bortolotti O, Torre E, Bidaud I, Lamb N, Fernandez A, Le Guennec JY, Mangoni ME, and Mesirca P
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- Animals, Calcium metabolism, Mice, Myocytes, Cardiac metabolism, Ryanodine Receptor Calcium Release Channel, Adrenergic Agents pharmacology, Sinoatrial Node metabolism
- Abstract
Background: Sinoatrial node cells (SANC) automaticity is generated by functional association between the activity of plasmalemmal ion channels and local diastolic intracellular Ca
2+ release (LCR) from ryanodine receptors. Strikingly, most isolated SANC exhibit a "dormant" state, whereas only a fraction shows regular firing as observed in intact SAN. Recent studies showed that β-adrenergic stimulation can initiate spontaneous firing in dormant SANC, though this mechanism is not entirely understood., Methods: To investigate the role of L-type Cav 1.3 Ca2+ channels in the adrenergic regulation of automaticity in dormant SANC, we used a knock-in mouse strain in which the sensitivity of L-type Cav 1.2 α1 subunits to dihydropyridines (DHPs) was inactivated ( Cav 1.2DHP-/- ), enabling the selective pharmacological inhibition of Cav 1.3 by DHPs., Results: In dormant SANC, β-adrenergic stimulation with isoproterenol (ISO) induced spontaneous action potentials (AP) and Ca2+ transients, which were completely arrested with concomitant perfusion of the DHP nifedipine. In spontaneously firing SANC at baseline, Cav 1.3 inhibition completely reversed the effect of β-adrenergic stimulation on AP and the frequency of Ca2+ transients. Confocal calcium imaging of SANC showed that the β-adrenergic-induced synchronization of LCRs is regulated by the activity of Cav 1.3 channels., Conclusions: Our study shows a novel role of Cav 1.3 channels in initiating and maintaining automaticity in dormant SANC upon β-adrenergic stimulation.- Published
- 2022
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25. Transient receptor potential vanilloid 4 channel participates in mouse ventricular electrical activity.
- Author
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Chaigne S, Cardouat G, Louradour J, Vaillant F, Charron S, Sacher F, Ducret T, Guinamard R, Vigmond E, and Hof T
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- Animals, Calcium Channels, L-Type metabolism, Computer Simulation, HEK293 Cells, Humans, Leucine analogs & derivatives, Leucine pharmacology, Male, Mice, Inbred C57BL, Mice, Knockout, Models, Cardiovascular, Myocytes, Cardiac drug effects, Piperidines pharmacology, Quinolines pharmacology, Sulfonamides pharmacology, TRPV Cation Channels deficiency, TRPV Cation Channels genetics, Time Factors, Mice, Action Potentials drug effects, Calcium Signaling drug effects, Heart Rate drug effects, Myocytes, Cardiac metabolism, TRPV Cation Channels metabolism, Ventricular Function, Left drug effects
- Abstract
The TRPV4 channel is a calcium-permeable channel ( P
Ca / PNa ∼ 10). Its expression has been reported in ventricular myocytes, where it is involved in several cardiac pathological mechanisms. In this study, we investigated the implication of TRPV4 in ventricular electrical activity. Left ventricular myocytes were isolated from trpv4+/+ and trpv4-/- mice. TRPV4 membrane expression and its colocalization with L-type calcium channels (Cav 1.2) was confirmed using Western blot biotinylation, immunoprecipitation, and immunostaining experiments. Then, electrocardiograms (ECGs) and patch-clamp recordings showed shortened QTc and action potential (AP) duration in trpv4-/- compared with trpv4+/+ mice. Thus, TRPV4 activator GSK1016790A produced a transient and dose-dependent increase in AP duration at 90% of repolarization (APD90 ) in trpv4+/+ but not in trpv4-/- myocytes or when combined with TRPV4 inhibitor GSK2193874 (100 nM). Hence, GSK1016790A increased calcium transient (CaT) amplitude in trpv4+/+ but not in trpv4-/- myocytes, suggesting that TRPV4 carries an inward Ca2+ current in myocytes. Conversely, TRPV4 inhibitor GSK2193874 (100 nM) alone reduced APD90 in trpv4+/+ but not in trpv4-/- myocytes, suggesting that TRPV4 prolongs AP duration in basal condition. Finally, introducing TRPV4 parameters in a mathematical model predicted the development of an inward TRPV4 current during repolarization that increases AP duration and CaT amplitude, in accord with what was found experimentally. This study shows for the first time that TRPV4 modulates AP and QTc durations. It would be interesting to evaluate whether TRPV4 could be involved in long QT-mediated ventricular arrhythmias. NEW & NOTEWORTHY Transient receptor potential vanilloid 4 (TRPV4) is expressed at the membrane of mouse ventricular myocytes and colocalizes with non-T-tubular L-type calcium channels. Deletion of trpv4 gene in mice results in shortened QT interval on electrocardiogram and reduced action potential duration of ventricular myocytes. Pharmacological activation of TRPV4 channel leads to increased action potential duration and increased calcium transient amplitude in trpv4-/- but not in trpv4-/- ventricular myocytes. To the contrary, TRPV4 channel pharmacological inhibition reduces action potential duration in trpv4+/+ but not in trpv4-/- myocytes. Integration of TRPV4 channel in a computational model of mouse action potential shows that the channel carries an inward current contributing to slowing down action potential repolarization and to increase calcium transient amplitude, similarly to what is observed experimentally. This study highlights for the first time the involvement of TRPV4 channel in ventricular electrical activity.- Published
- 2021
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26. Concomitant genetic ablation of L-type Ca v 1.3 (α 1D ) and T-type Ca v 3.1 (α 1G ) Ca 2+ channels disrupts heart automaticity.
- Author
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Baudot M, Torre E, Bidaud I, Louradour J, Torrente AG, Fossier L, Talssi L, Nargeot J, Barrère-Lemaire S, Mesirca P, and Mangoni ME
- Subjects
- Animals, Bradycardia genetics, Bradycardia physiopathology, Calcium metabolism, Disease Models, Animal, Electrocardiography, Heart Rate, Mice, Mice, Knockout, Sarcoplasmic Reticulum metabolism, Atrioventricular Node physiopathology, Bradycardia diagnosis, Calcium Channels, L-Type genetics, Calcium Channels, T-Type genetics, Sinoatrial Node physiopathology
- Abstract
Cardiac automaticity is set by pacemaker activity of the sinus node (SAN). In addition to the ubiquitously expressed cardiac voltage-gated L-type Ca
v 1.2 Ca2+ channel isoform, pacemaker cells within the SAN and the atrioventricular node co-express voltage-gated L-type Cav 1.3 and T-type Cav 3.1 Ca2+ channels (SAN-VGCCs). The role of SAN-VGCCs in automaticity is incompletely understood. We used knockout mice carrying individual genetic ablation of Cav 1.3 (Cav 1.3-/- ) or Cav 3.1 (Cav 3.1-/- ) channels and double mutant Cav 1.3-/- /Cav 3.1-/- mice expressing only Cav 1.2 channels. We show that concomitant loss of SAN-VGCCs prevents physiological SAN automaticity, blocks impulse conduction and compromises ventricular rhythmicity. Coexpression of SAN-VGCCs is necessary for impulse formation in the central SAN. In mice lacking SAN-VGCCs, residual pacemaker activity is predominantly generated in peripheral nodal and extranodal sites by f-channels and TTX-sensitive Na+ channels. In beating SAN cells, ablation of SAN-VGCCs disrupted late diastolic local intracellular Ca2+ release, which demonstrates an important role for these channels in supporting the sarcoplasmic reticulum based "Ca2+ clock" mechanism during normal pacemaking. These data implicate an underappreciated role for co-expression of SAN-VGCCs in heart automaticity and define an integral role for these channels in mechanisms that control the heartbeat.- Published
- 2020
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27. Suitability of V1 energy models for object classification.
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Bergstra J, Bengio Y, and Louradour J
- Subjects
- Artificial Intelligence, Computer Simulation, Humans, Nonlinear Dynamics, Discrimination, Psychological physiology, Models, Neurological, Neurons physiology, Pattern Recognition, Visual physiology, Visual Cortex physiology
- Abstract
Simulations of cortical computation have often focused on networks built from simplified neuron models similar to rate models hypothesized for V1 simple cells. However, physiological research has revealed that even V1 simple cells have surprising complexity. Our computational simulations explore the effect of this complexity on the visual system's ability to solve simple tasks, such as the categorization of shapes and digits, after learning from a limited number of examples. We use recently proposed high-throughput methodology to explore what axes of modeling complexity are useful in these categorization tasks. We find that complex cell rate models learn to categorize objects better than simple cell models, and without incurring extra computational expense. We find that the squaring of linear filter responses leads to better performance. We find that several other components of physiologically derived models do not yield better performance.
- Published
- 2011
- Full Text
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