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2. Recommendations for recognizing, risk stratifying, treating, and managing children and adolescents with hypoglycemia

Author

Stefano Zucchini, Stefano Tumini, Andrea Enzo Scaramuzza, Riccardo Bonfanti, Maurizio Delvecchio, Roberto Franceschi, Dario Iafusco, Lorenzo Lenzi, Enza Mozzillo, Stefano Passanisi, Claudia Piona, Ivana Rabbone, Novella Rapini, Andrea Rigamonti, Carlo Ripoli, Giuseppina Salzano, Silvia Savastio, Riccardo Schiaffini, Angela Zanfardino, Valentino Cherubini, Diabetes Study Group of the Italian Society for Pediatric Endocrinology Diabetes, Albino Claudia Accursia, Aloe Monica, Anzelotti Maria Teresa, Arnaldi Claudia, Barbetti Fabrizio, Bassi Marta, Berioli Maria Giulia, Bernardini Luca, Bertelli Enrica, Biagioni Martina, Bobbio Adriana, Bombaci Bruno, Bonfanti Riccardo, Bonura Clara, Bracciolini Giulia Patrizia, Bruzzese Mariella, Bruzzi Patrizia, Buono Pietro, Buscarino Piera, Cadario Francesco, Calcaterra Valeria, Calzi Elena, Cappa Marco, Cardani Roberta, Cardella Francesca, Cardinale Giuliana Marcella, Casertano Alberto, Castorani Valeria, Cauvin Vittoria, Cenciarelli Valentina, Ceruti Franco, Cherubini Valentino, Chiarelli Francesco, Chiari Giovanni, Cianfarani Stefano, Cicchetti Mario, Cipriano Paola, Cirillo Dante, Citriniti Felice, Coccioli Maria Susanna, Confetto Santino, Contreas Giovanna, Coro Anna, Correddu Antonella, Corsini Elisa, Crino’ Antonino, d’Annunzio Giuseppe, De Berardinis Fiorella, De Donno Valeria, De Filippo Gianpaolo, De Marco Rosaria, De Sanctis Luisa, Del Duca Elisabetta, Delvecchio Maurizio, Deodati Annalisa, Di Bonito Procolo, Di Candia Francesca, Faleschini Elena, Fattorusso Valentina, Favia Anna, Federico Giovanni, Felappi Barbara, Ferrari Mara, Ferrito Lucia, Fichera Graziella, Fontana Franco, Fornari Elena, Franceschi Roberto, Franco Francesca, Franzese Adriana, Frongia Anna Paola, Frontino Giulio, Gaiero Alberto, Galassi Sabrina Maria, Gallo Francesco, Gargantini Luigi, Giani Elisa, Gortan Anna Jolanda, Graziani Vanna, Grosso Caterina, Gualtieri Antonella, Guasti Monica, Guerraggio Lucia Paola, Guzzetti Chiara, Iafusco Dario, Iannicelli Gennaro, Iezzi Maria Laura, Ignaccolo Maria Giovanna, Innaurato Stefania, Inzaghi Elena, Iovane Brunella, Iughetti Lorenzo, Kaufmann Peter, La Loggia Alfonso, Lambertini Anna Giulia, Lapolla Rosa, Lasagni Anna, Lazzaro Nicola, Lazzeroni Pietro, Lenzi Lorenzo, Lera Riccardo, Levantini Gabriella, Lezzi Marilea, Lia Rosanna, Liguori Alice, Lo Presti Donatella, Lombardo Fortunato, Lonero Antonella, Longhi Silvia, Lorubbio Antonella, Lucchesi Sonia, Maccioni Rosella, Macedoni Maddalena, Macellaro Patrizia Cristiana, Madeo Simona Filomena, Maffeis Claudio, Mainetti Benedetta, Maltoni Giulio, Mameli Chiara, Mammì Francesco, Manca Bitti Maria Luisa, Mancioppi Valentina, Manco Melania, Marigliano Marco, Marino Monica, Marsciani Alberto, Matteoli Maria Cristina, Mazzali Elena, Minute Marta, Minuto Nicola, Monti Sara, Morandi Anita,, Morganti Gianfranco, Morotti Elisa, Mozzillo Enza, Musolino Gianluca, Olivieri Francesca, Ortolani Federica, Pampanini Valentina, Pardi Daniela, Pascarella Filomena, Pasquino Bruno, Passanisi Stefano, Patera Ippolita Patrizia, Pedini Annalisa, Pennati Maria Cristina, Peruzzi Sonia, Peverelli Paola, Pezzino Giulia, Piccini Barbara, Piccinno Elvira Eugenia Rosaria, Piona Claudia, Piredda Gavina, Piscopo Alessia, Pistone Carmelo, Pozzi Erica, Prandi Elena, Predieri Barbara, Prudente Sabrina, Pulcina Anna, Rabbone Ivana, Randazzo Emioli, Rapini Novella, Reinstadler Petra, Riboni Sara, Ricciardi Maria Rossella, Rigamonti Andrea, Ripoli Carlo, Rossi Virginia, Rossi Paolo, Rutigliano Irene, Sabbion Alberto, Salvatoni Alessandro, Salvo Caterina, Salzano Giuseppina, Sanseviero Mariateresa, Savastio Silvia, Savini Rosanna, Scanu Mariapiera, Scaramuzza Andrea Enzo, Schiaffini Riccardo, Schiavone Maurizio, Schieven Eleonardo, Scipione Mirella, Secco Andrea, Silvestri Francesca, Siri Giulia, Sogno Valin Paola, Sordelli Silvia, Spiri Daniele, Stagi Stefano, Stamati Filomena Andreina, Suprani Tosca, Talarico Valentina, Tiberi Valentina, Timpanaro Tiziana Antonia Lucia, Tinti Davide, Tirendi Antonina, Tomaselli Letizia Grazia, Toni Sonia, Torelli Cataldo, Tornese Gianluca, Trada Michela,, Trettene Adolfo Andrea, Tumini Stefano, Tumminelli Marilena, Valerio Giuliana, Vandelli Sara, Ventrici Claudia, Zampolli Maria, Zanatta Manuela, Zanfardino Angela, Zecchino Clara, Zonca Silvia, and Zucchini Stefano

Subjects
adolescents, automated insulin delivery, children, hypoglycemia, glucagon, oral glucose, Diseases of the endocrine glands. Clinical endocrinology, RC648-665
Abstract

There has been continuous progress in diabetes management over the last few decades, not least due to the widespread dissemination of continuous glucose monitoring (CGM) and automated insulin delivery systems. These technological advances have radically changed the daily lives of people living with diabetes, improving the quality of life of both children and their families. Despite this, hypoglycemia remains the primary side-effect of insulin therapy. Based on a systematic review of the available scientific evidence, this paper aims to provide evidence-based recommendations for recognizing, risk stratifying, treating, and managing patients with hypoglycemia. The objective of these recommendations is to unify the behavior of pediatric diabetologists with respect to the timely recognition and prevention of hypoglycemic episodes and the correct treatment of hypoglycemia, especially in patients using CGM or advanced hybrid closed-loop systems. All authors have long experience in the specialty and are members of the Italian Society of Pediatric Endocrinology and Diabetology. The goal of treating hypoglycemia is to raise blood glucose above 70 mg/dL (3.9 mmol/L) and to prevent further decreases. Oral glucose at a dose of 0.3 g/kg (0.1 g/kg for children using “smart pumps” or hybrid closed loop systems in automated mode) is the preferred treatment for the conscious individual with blood glucose

Published
2024
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4. Isolated childhood growth hormone deficiency: a 30-year experience on final height and a new prediction model

Author

Lonero, Antonella, Giotta, Massimo, Guerrini, Giulia, Calcaterra, Valeria, Galazzi, Elena, Iughetti, Lorenzo, Cassio, Alessandra, Wasniewska, Gabriela Malgorzata, Mameli, Chiara, Tornese, Gianluca, Salerno, Mariacarolina, Cherubini, Valentino, Caruso Nicoletti, Manuela, Street, Maria Elisabeth, Grandone, Anna, Giacomozzi, Claudio, Faienza, Maria Felicia, Guzzetti, Chiara, Bellone, Simonetta, Parpagnoli, Maria, Musolino, Gianluca, Maggio, Maria Cristina, Bozzola, Mauro, Trerotoli, Paolo, and Delvecchio, Maurizio

Published
2022
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5. Entropy Analysis of the Bioelectrical Activity of Plants

Author

Muller, Hartmut, Baccara, Roberta, Hofmann, Rose Line, Lonero, Giuseppe, Muratori, Simona, Papa, Giuliana, Santoni, Francesca, Todesco, Loretta, Zanellati, Francesco, and Khosravi, Leili

Subjects
Plants -- Analysis, Electromagnetic fields -- Analysis, Electromagnetism -- Analysis, Neurophysiology -- Analysis, Physics
Abstract

The paper introduces entropy analysis of bioelectrical activity based on harmonic signal distortion. Over a period of one year, the ultradian dynamics of the electrical activity of higher plants and the response on low frequency electromagnetic fields and modulated light were recorded. Biological active frequencies increasing or decreasing the entropy of bioelectrical activity were identified., Introduction Bioelectricity are electric potentials and currents produced by or occurring within living organisms. The experiments of Luigi Galvani and Alessandro Volta in the 18th century proved the connection between [...]

Published
2021

6. Uniparental disomy and pretreatment IGF-1 may predict elevated IGF-1 levels in Prader-Willi patients on GH treatment

Author

Palmieri, Viviana Valeria, Lonero, Antonella, Bocchini, Sarah, Cassano, Gilda, Convertino, Alessio, Corica, Domenico, Crinò, Antonio, Fattorusso, Valentina, Ferraris, Silvio, Fintini, Danilo, Franzese, Adriana, Grugni, Graziano, Iughetti, Lorenzo, Lia, Rosanna, Macchi, Francesca, Madeo, Simona Filomena, Matarazzo, Patrizia, Nosetti, Luana, Osimani, Sara, Pajno, Roberta, Patti, Giuseppa, Pellegrin, Maria Chiara, Perri, Annamaria, Ragusa, Letizia, Rutigliano, Irene, Sacco, Michele, Salvatoni, Alessandro, Scarano, Emanuela, Stagi, Stefano, Tornese, Gianluca, Trifirò, Giuliana, Wasniewska, Malgorzata, Fischetto, Rita, Giordano, Paola, Licenziati, Maria Rosaria, and Delvecchio, Maurizio

Published
2019
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7. Forensic Analysis and Identification Processes in Mass Disasters: Explosion of Gun Powder in the Fireworks Factory

Author

Maricla Marrone, Francesca Tarantino, Alessandra Stellacci, Stefania Lonero Baldassarra, Gerardo Cazzato, Francesco Vinci, and Alessandro Dell’Erba

Subjects
forensic analyzes, DNA identification, mass disasters, reconstruction, Organic chemistry, QD241-441
Abstract

A mass disaster is a situation that involves criticality between the number of victims and resources, in terms of both men and means, present on the site of an event that is mostly unexpected and sudden. In the multidisciplinary teams that intervene, the role of forensic pathologists, who are responsible for the direction and coordination of post-mortem operations, is central, and must remain so. The authors report the case of an explosion of a pyrotechnic artifice factory, as a result of which numerous victims and injuries are recorded. So, the team completed the autopsies and created a protocol to obtain biological samples (bones, blood, teeth, muscles), while the forensic pathologists contacted the families of the alleged victims and each provided a blood sample that was collected for the DNA. The geneticist, using the method of gene extraction and amplification, obtained the DNA from each bone, tooth, and muscle of blood taken from the victims and then compared it with that extracted from the blood samples of the relatives; the electropherograms showed at least one allele for each genetic marker of the “Combined DNA Index System” in common between the victims and the families, thus allowing to establish the identity of all the subjects involved in the event. Having established the identity of all workers, it was possible to determine their whereabouts in the environment at the time of the location of fires and explosions. The results of the various forensic analyzes (autopsies, genetic investigations and even traumatological investigations) have allowed us to validate a scientific method useful in all mass disasters even when any type of anthropological or forensic dental research is difficult.

Published
2021
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8. Death Related to a Congenital Vascular Anomaly of Pulmonary Hamartoma Type: Malpractice or Tragic Fatality?

Author

Maricla Marrone, Laura Marrone, Gerardo Cazzato, Stefania Lonero Baldassarra, Giuseppe Ingravallo, and Alessandra Stellacci

Subjects
medical malpractice, defensive medicine, bronchoscopy, doctor-patient relationship, autopsy, COVID-19, Medicine (General), R5-920
Abstract

In forensic pathology, apparently straightforward cases can often hide rarities that, if not correctly interpreted, can alter the results of the entire investigation, leading to misinterpretations. This occurs when the investigation is conducted to assess medical malpractice. An unexpected death, with no known apparent cause, is often linked to an underlying disease process of unclear etiological origin whose nature can, unfortunately, be properly investigated only post-mortem. This presentation shows a case study, in which it was possible to reconduct the death of a patient to a natural pathology and not to medical treatment. Here, the authors illustrate a case with a hamartoma developed in chronic inflammatory conditions (bronchiectasis) that was difficult to differentiate from lung cancer due to the inability to perform specific instrumental examinations. The hamartoma, usually benign and identifiable by standard instrumental investigations, in this case, led to the patient’s death precisely during the execution of a bronchoscopy. However, in the absence of a certain cause of death, public opinion unanimously attributes a patient’s disease to medical error. Indeed, a routine practice such as bronchoscopy should not cause death and consequently, the doctor must have made a mistake. Fortunately, the autopsy not only demonstrated the origin of the bleeding but also unveiled the reason for this, as rare congenital lung disease. Fate, one might say.

Published
2021
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10. Efficacy and safety of growth hormone treatment in children with short stature: the Italian cohort of the GeNeSIS clinical study

Author

Cappa, M., Iughetti, L., Loche, S., Maghnie, M., Vottero, A., Antoniazzi, Franco, Beccaria, Luciano, Bernasconi, Sergio, Caggiano, Domenico, Caruso-Nicoletti, Manuela, Catucci, Alessandra, Chiarelli, Francesco, Cianfarani, Stefano, Colucci, Anna Rita, De Rienzo, Francesca, Di Pumpo, Raffaele, Di Stasio, Alessandra, Farello, Giovanni, Felici, Leonardo, Femiano, Pasquale, Garagantini, Luigi, Giavoli, Claudia, Greggio, Nella Augusta, Guazzarotti, Laura, Larizza, Daniela, Licenziati, Maria Rosaria, Lonero, Antonella, Maggio, Maria Cristina, Marsciani, Alberto, Matarazzo, Patrizia, Mazzanti, Laura, Messini, Beatrice, Napoli, Flavia, Pasquino, Anna Maria, Perrone, Laura, Pilia, Sabrina, Pilotta, Alba, Piran, Marzia, Pozzobon, Gabriella, Predieri, Barbara, Sacco, Michele, Salerno, Mariacarolina, Tirendi, Antonina, Ubertini, Graziamaria, Vannelli, Silvia, Wasniewska, Malgorzata, Zampolli, Maria, Zanotti, Martina, Zuccotti, Gianvincenzo, and GeNeSIS National Board on behalf of the GeNeSIS Italian Investigators

Published
2016
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13. Death Related to a Congenital Vascular Anomaly of Pulmonary Hamartoma Type: Malpractice or Tragic Fatality?

Author

Giuseppe Ingravallo, Gerardo Cazzato, Alessandra Stellacci, Maricla Marrone, Stefania Lonero Baldassarra, and Laura Marrone

Subjects
Medicine (General), medicine.medical_specialty, Forensic pathology, defensive medicine, bronchoscopy, business.industry, Medical malpractice, medical malpractice, COVID-19, Autopsy, Case Report, General Medicine, Disease, medicine.disease, doctor-patient relationship, Defensive medicine, R5-920, autopsy, Malpractice, medicine, Hamartoma, Intensive care medicine, business, Cause of death
Abstract

In forensic pathology, apparently straightforward cases can often hide rarities that, if not correctly interpreted, can alter the results of the entire investigation, leading to misinterpretations. This occurs when the investigation is conducted to assess medical malpractice. An unexpected death, with no known apparent cause, is often linked to an underlying disease process of unclear etiological origin whose nature can, unfortunately, be properly investigated only post-mortem. This presentation shows a case study, in which it was possible to reconduct the death of a patient to a natural pathology and not to medical treatment. Here, the authors illustrate a case with a hamartoma developed in chronic inflammatory conditions (bronchiectasis) that was difficult to differentiate from lung cancer due to the inability to perform specific instrumental examinations. The hamartoma, usually benign and identifiable by standard instrumental investigations, in this case, led to the patient’s death precisely during the execution of a bronchoscopy. However, in the absence of a certain cause of death, public opinion unanimously attributes a patient’s disease to medical error. Indeed, a routine practice such as bronchoscopy should not cause death and consequently, the doctor must have made a mistake. Fortunately, the autopsy not only demonstrated the origin of the bleeding but also unveiled the reason for this, as rare congenital lung disease. Fate, one might say.

Published
2021

15. Novice Driver Education Model Curriculum Outline.

Author

Lonero, Lawrence

Abstract

This document, which was developed after a comprehensive review of the current state of driver education across the United States and which included an extensive literature review and interviews with 40 individuals from various sectors, including education, law enforcement, and the insurance industry, identifies ways of revamping driver education. Discussed in the introduction are the current state of knowledge and the need to rethink the objectives and methods of driver education. The following aspects of developing a curriculum outline strategy are considered: driver education's missions, stakeholder needs, underlying strategic assumptions, and curriculum development goals. Presented next is an outline listing 40 performance objectives in the following categories: motivation, knowledge, attention, detection, perception, evaluation, decision, motor skills, safety margin, and responsibility. The following topics are examined in a section on methods: shaping methods to goals, building instructional media units, instructional delivery, refocusing driver education resources on motivation, educating motivation and responsibility, planning and evaluation, and curriculum integration. Included in a section on supporting noninstructional influences are suggestions for coordinating community influences and linking driver education with graduated licensing. Concluding the document are a summary, and 10 recommendations for revamping driver education. Appended are a methods outline and list of the 40 experts consulted. Contains 136 references. (MN)

Published
1995

16. Evaluation of Beginner Driver Education in Oregon

Author

Dan Mayhew, Ward Vanlaar, Larry Lonero, Robyn Robertson, Kyla Marcoux, Katherine Wood, Kathryn Clinton, and Herb Simpson

Subjects
driver education (DE), beginner drivers, teen drivers, evaluation, safety effects, crashes, road safety, Industrial safety. Industrial accident prevention, T55-55.3, Medicine (General), R5-920
Abstract

Although driver education (DE) is widely accepted as an effective teen driver safety measure and widely available in the United States, Canada and elsewhere, evaluations have generally failed to show that such formal programs actually produce safer drivers. To address the issue of safety effects as part of a larger investigation, two studies were conducted to examine whether the Oregon Department of Transportation (ODOT)-approved DE program was associated with reductions in collisions and convictions. In the first study, DE status among a relatively small sample of teens who completed an online survey was not found to have a significant effect on collisions and convictions. In the second study, of a much larger population of teen drivers, DE status was associated with a lower incidence of collisions and convictions. On balance, this suggests that the safety effects of DE are either neutral, based on the results of the first Oregon study, or cautiously optimistic based on the results of the second study. The implications of these findings are discussed in terms of making improvements in DE that are evidence-based, and the need for further evaluation to establish that improved and new programs meet their safety objectives.

Published
2017
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17. Identification of Skeletal Remains Using Genetic Profiling: A Case Linking Italy and Poland.

Author

Tarantino, Francesca, Buongiorno, Luigi, Luca, Benedetta Pia De, Stellacci, Alessandra, Landro, Michele Di, Sebastiani, Gabriele Vito, Cazzato, Gerardo, Baldassarra, Stefania Lonero, Nuzzolese, Emilio, and Marrone, Maricla

Subjects
GENETIC profile, ANTHROPOMETRY, FORENSIC genetics, GOVERNMENTAL investigations, GENETIC databases, GENETIC markers
Abstract

Forensic genetics is a rapidly evolving science thanks to the growing variety of genetic markers, the establishment of faster, less error-prone sequencing technologies, and the engineering of bioinformatics models, methods, and structures. In the early 2000s, the need emerged to create an international genetic database for forensic purposes. This paper describes a judicial investigation of skeletal remains to identify the subject using various methods. The anthropological examination of the remains allowed identification of the Caucasoid (European) ethnic group, a height of 156 ± 4 cm, and an age between 47 and 50 years. The genetic profiles obtained from typing several microsatellites made it possible to evaluate the compatibility between the skeletal remains and the suspected decedent. To identify the remains, the two extrapolated genetic profiles were compared. The case described highlights the central role of forensic genetics in identifying skeleton remains by means of comparison. [ABSTRACT FROM AUTHOR]

Published
2023
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20. Differences between Transient Neonatal Diabetes Mellitus Subtypes can Guide Diagnosis and Therapy

Author

Riccardo Bonfanti, Dario Iafusco, Ivana Rabbone, Giacomo Diedenhofen, Carla Bizzarri, Patrizia Ippolita Patera, Petra Reinstadler, Francesco Costantino, Valeria Calcaterra, Lorenzo Iughetti, Silvia Savastio, Anna Favia, Francesca Cardella, Donatella Lo Presti, Ylenia Girtler, Sarah Rabbiosi, Giuseppe D’Annunzio, Angela Zanfardino, Alessia Piscopo, Francesca Casaburo, Letizia Pintomalli, Lucia Russo, Valeria Grasso, Nicola Minuto, Mafalda Mucciolo, Antonio Novelli, Antonella Marucci, Barbara Piccini, Sonia Toni, Francesca Silvestri, Paola Carrera, Andrea Rigamonti, Giulio Frontino, Michela Trada, Davide Tinti, Maurizio Delvecchio, Novella Rapini, Riccardo Schiaffini, Corrado Mammì, Fabrizio Barbetti, Monica Aloe, Simona Amadeo, Claudia Arnaldi, Marta Bassi, Luciano Beccaria, Marzia Benelli, Giulia Maria Berioloi, Enrica Bertelli, Martina Biagioni, Adriana Bobbio, Stefano Boccato, Oriana Bologna, Franco Bontempi, Clara Bonura, Giulia Bracciolini, Claudia Brufani, Patrizia Bruzzi, Pietro Buono, Roberta Cardani, Giuliana Cardinale, Alberto Casertano, Maria Cristina Castiglione, Vittoria Cauvin, Valentino Cherubini, Franco Chiarelli, Giovanni Chiari, Stefano Cianfarani, Dante Cirillo, Felice Citriniti, Susanna Coccioli, Anna Cogliardi, Santino Confetto, Giovanna Contreas, Anna Corò, Elisa Corsini, Nicoletta Cresta, Fiorella De Berardinis, Valeria De Donno, Giampaolo De Filippo, Rosaria De Marco, Annalisa Deodati, Elena Faleschini, Valentina Fattorusso, Valeria Favalli, Barbara Felappi, Lucia Ferrito, Graziella Fichera, Franco Fontana, Elena Fornari, Roberto Franceschi, Francesca Franco, Adriana Franzese, Anna Paola Frongia, Alberto Gaiero, Francesco Gallo, Luigi Gargantini, Elisa Giani, Chiara Giorgetti, Giulia Bianchi, Vanna Graziani, Antonella Gualtieri, Monica Guasti, Gennaro Iannicelli, Antonio Iannilli, Ignaccolo Giovanna, Dario Ingletto, Stefania Innaurato, Elena Inzaghi, Brunella Iovane, Peter Kaufmann, Alfonso La Loggia, Rosa Lapolla, Anna Lasagni, Nicola Lazzaro, Lorenzo Lenzi, Riccardo Lera, Gabriella Levantini, Fortunato Lombardo, Antonella Lonero, Silvia Longhi, Sonia Lucchesi, Lucia Paola Guerraggio, Sergio Lucieri, Patrizia Macellaro, Claudio Maffeis, Bendetta Mainetti, Giulio Maltoni, Chiara Mameli, Francesco Mammì, Maria Luisa Manca-Bitti, Melania Manco, Monica Marino, Matteo Mariano, Marco Marigliano, Alberto Marsciani, Costanzo Mastrangelo, Maria Cristina Matteoli, Elena Mazzali, Franco Meschi, Antonella MIgliaccio, Anita Morandi, Gianfranco Morganti, Enza Mozzillo, Gianluca Musolino, Rosa Nugnes, Federica Ortolani, Daniela Pardi, Filomena Pascarella, Stefano Passanisi, Annalisa Pedini, Cristina Pennati, Angelo Perrotta, Sonia Peruzzi, Paola Peverelli, Giulia Pezzino, Anita Claudia Piona, Gavina Piredda, Carmelo Pistone, Elena Prandi, Barbara Pedieri, Procolo Di Bonito, Anna Pulcina, Maria Quinci, Emioli Randazzo, Rossella Ricciardi, Carlo Ripoli, Rosanna Roppolo, Irene Rutigliano, Alberto Sabbio, Silvana salardi, Alessandro Salvatoni, Anna Saporiti, Rita Sardi, Mariapiera Scanu, Andrea Scaramuzza, Eleonardo Schiven, Andrea Secco, Linda Sessa, Paola Sogno Valin, Silvia Sordelli, Luisa Spallino, Stefano Stagi, Filomena Stamati, Tosca Suprani, Valentina Talarico, Tiziana Timapanaro, Antonella Tirendi, Letizia Tomaselli, Gianluca Tornese, Adolfo Andrea Trettene, Stefano Tumini, Giuliana Valerio, Claudia Ventrici, Matteo Viscardi, Silvana Zaffani, Maria Zampolli, Giorgio Zanette, Clara Zecchino, Maria Antonietta Zedda, Silvia Zonca, Stefano Zucchini, Bonfanti, R., Iafusco, D., Rabbone, I., Diedenhofen, G., Bizzarri, C., Patera, P. I., Reinstadler, P., Costantino, F., Calcaterra, V., Iughetti, L., Savastio, S., Favia, A., Cardella, F., Presti, D. L., Girtler, Y., Rabbiosi, S., D'Annunzio, G., Zanfardino, A., Piscopo, A., Casaburo, F., Pintomalli, L., Russo, L., Grasso, V., Minuto, N., Mucciolo, M., Novelli, A., Marucci, A., Piccini, B., Toni, S., Silvestri, F., Carrera, P., Rigamonti, A., Frontino, G., Trada, M., Tinti, D., Delvecchio, M., Rapini, N., Schiaffini, R., Mammi, C., and Barbetti, F.

Subjects
Proband, Male, Pediatrics, Potassium Channels, Endocrinology, Diabetes and Metabolism, Datasets as Topic, Diagnosis, Differential, Diagnostic Techniques, Endocrine, Female, Humans, Infant, Infant, Newborn, Italy, Mutation, Potassium Channels, Inwardly Rectifying, Remission Induction, Retrospective Studies, Sulfonylurea Receptors, Diabetes Mellitus, Infant, Newborn, Diseases, Diseases, Gastroenterology, Diabetes mellitus genetics, Endocrinology, Settore MED/13, Retrospective Studie, Diagnosis, Medicine, Endocrine pancreas, Transient Neonatal Diabetes Mellitus, 6q24 TNDM, KATP TNDM, Sulfonylureas, Sulfonylureas, Sulfonylurea Receptor, biology, Diabetes Mellitu, General Medicine, Metformin, Inwardly Rectifying, Settore MED/03, 6q24 TNDM, medicine.symptom, Endocrine, hormones, hormone substitutes, and hormone antagonists, medicine.drug, Human, endocrine system, medicine.medical_specialty, KATP TNDM, ABCC8, Transient Neonatal Diabetes Mellitus, Internal medicine, Diabetes mellitus, Macroglossia, Endocrine pancreas, business.industry, medicine.disease, Newborn, Diagnostic Techniques, Transient neonatal diabetes mellitus, Differential, biology.protein, Sulfonylurea receptor, business
Abstract

Objective Transient neonatal diabetes mellitus (TNDM) is caused by activating mutations in ABCC8 and KCNJ11 genes (KATP/TNDM) or by chromosome 6q24 abnormalities (6q24/TNDM). We wanted to assess whether these different genetic aetiologies result in distinct clinical features. Design Retrospective analysis of the Italian data set of patients with TNDM. Methods Clinical features and treatment of 22 KATP/TNDM patients and 12 6q24/TNDM patients were compared. Results Fourteen KATP/TNDM probands had a carrier parent with abnormal glucose values, four patients with 6q24 showed macroglossia and/or umbilical hernia. Median age at diabetes onset and birth weight were lower in patients with 6q24 (1 week; −2.27 SD) than those with KATP mutations (4.0 weeks; −1.04 SD) (P = 0.009 and P = 0.007, respectively). Median time to remission was longer in KATP/TNDM than 6q24/TNDM (21.5 weeks vs 12 weeks) (P = 0.002). Two KATP/TNDM patients entered diabetes remission without pharmacological therapy. A proband with the ABCC8/L225P variant previously associated with permanent neonatal diabetes entered 7-year long remission after 1 year of sulfonylurea therapy. Seven diabetic individuals with KATP mutations were successfully treated with sulfonylurea monotherapy; four cases with relapsing 6q24/TNDM were treated with insulin, metformin or combination therapy. Conclusions If TNDM is suspected, KATP genes should be analyzed first with the exception of patients with macroglossia and/or umbilical hernia. Remission of diabetes without pharmacological therapy should not preclude genetic analysis. Early treatment with sulfonylurea may induce long-lasting remission of diabetes in patients with KATP mutations associated with PNDM. Adult patients carrying KATP/TNDM mutations respond favourably to sulfonylurea monotherapy.

Published
2021

21. Slow and Steady Wins the Race: A Comparative Analysis of Standing Electric Scooters' European Regulations Integrated with the Aspect of Forensic Traumatology.

Author

Buongiorno, Luigi, Stellacci, Alessandra, Cazzato, Gerardo, Caricato, Pierluigi, Luca, Benedetta Pia De, Tarantino, Francesca, Baldassarra, Stefania Lonero, Ingravallo, Giuseppe, and Marrone, Maricla

Abstract

Fuel-driven cars are widely considered unsustainable and contrary to the new paradigm of smart growth planning. The need to reform transport behavior, policies, and infrastructure is among the priorities in urban policies around the world. Electric vehicles are an emerging technology that could advance sustainability programs. In the past year, there has been a rapid increase in the diffusion of electric scooters in several European cities, but various states have been unprepared for the rapid spread of green micro-mobility from a regulatory point of view. In addition, in parallel with the spread, there have been numerous road collisions involving standing electric scooters. The aim of this study was to obtain a detailed view of this phenomenon. We focused on the current legislation on electric micro-mobility at the European level to study and summarize the different attitudes adopted by various states whose regulations are present on the web. (It was not possible to evaluate the regulations of all European countries because they are not all available on online platforms.) The elements assessed in the various regulation were age limits, speed limits, compulsory use of helmets, administrative penalties, and the obligation to insure the new e-vehicle (standing scooter). In this study, we analyze the state of the art in electric micro-mobility, highlight the current situation's limits, and propose new strategies to adequately integrate this new smart vehicle into the urban transport network. [ABSTRACT FROM AUTHOR]

Published
2022
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23. Uniparental disomy and pretreatment IGF-1 may predict elevated IGF-1 levels in Prader-Willi patients on GH treatment

Author

Malgorzata Wasniewska, Maria Chiara Pellegrin, Rita Fischetto, Francesca Macchi, Antonella Lonero, Sara Osimani, A. Crinò, Adriana Franzese, Sarah Bocchini, Gilda Cassano, Luana Nosetti, Annamaria Perri, Giuseppa Patti, Maria Rosaria Licenziati, Michele Sacco, Stefano Stagi, Alessandro Salvatoni, G. Trifirò, Rosanna Lia, Simona Filomena Madeo, Irene Rutigliano, Lorenzo Iughetti, Paola Giordano, Danilo Fintini, Gianluca Tornese, Alessio Convertino, Patrizia Matarazzo, Graziano Grugni, S. Ferraris, Emanuela Scarano, Domenico Corica, Valentina Fattorusso, Viviana Valeria Palmieri, Roberta Pajno, L. Ragusa, Maurizio Delvecchio, Palmieri, V. V., Lonero, A., Bocchini, S., Cassano, G., Convertino, A., Corica, D., Crino, A., Fattorusso, V., Ferraris, S., Fintini, D., Franzese, A., Grugni, G., Iughetti, L., Lia, R., Macchi, F., Madeo, S. F., Matarazzo, P., Nosetti, L., Osimani, S., Pajno, R., Patti, G., Pellegrin, M. C., Perri, A., Ragusa, L., Rutigliano, I., Sacco, M., Salvatoni, A., Scarano, E., Stagi, S., Tornese, G., Trifiro, G., Wasniewska, M., Fischetto, R., Giordano, P., Licenziati, M. R., and Delvecchio, M.

Subjects
Male, 0301 basic medicine, medicine.medical_specialty, Adolescent, Endocrinology, Diabetes and Metabolism, Growth hormone therapy, 030209 endocrinology & metabolism, Adverse effect, Gastroenterology, Growth velocity, 03 medical and health sciences, Adverse effects, IGF-1, Prader-Willi syndrome, Uniparental disomy, Child, Child, Preschool, Female, Human Growth Hormone, Humans, Infant, Insulin-Like Growth Factor I, Prader-Willi Syndrome, Prognosis, Uniparental Disomy, 0302 clinical medicine, Endocrinology, Internal medicine, medicine, Preschool, Normal range, business.industry, Human growth hormone, medicine.disease, Highly sensitive, 030104 developmental biology, Gh treatment, business
Abstract

Pediatric patients with Prader-Willi syndrome (PWS) can be treated with recombinant human GH (rhGH). These patients are highly sensitive to rhGH and the standard doses suggested by the international guidelines often result in IGF-1 above the normal range. We aimed to evaluate 1 the proper rhGH dose to optimize auxological outcomes and to avoid potential overtreatment, and 2 which patients are more sensitive to rhGH. In this multicenter real-life study, we recruited 215 patients with PWS older than 1 year, on rhGH at least for 6 months, from Italian Centers for PWS care. We collected auxological parameters, rhGH dose, IGF-1 at recruitment and (when available) at start of treatment. The rhGH dose was 4.3 (0.7/8.4) mg/m2/week. At recruitment, IGF-1 was normal in 72.1% and elevated in 27.9% of the patients. In the group of 115 patients with IGF-1 available at start of rhGH, normal pretreatment IGF-1 and uniparental disomy were associated with elevated IGF-1 during the therapy. No difference in height and growth velocity was found between patients treated with the highest and the lowest range dose. The rhGH dose prescribed in Italy seems lower than the recommended one. Normal pretreatment IGF-1 and uniparental disomy are risk factors for elevated IGF-1. The latter seems to be associated with higher sensitivity to GH. In case of these risk factors, we recommend a more accurate titration of the dose to avoid overtreatment and its potential side effects.

Published
2019

24. Final height in GH-deficient paediatric patients: a nationwide experience

Author

Zucchini, S, Lonero, A, Bellone, S, Bozzola, M, Cassio, A, Faienza, Mf, Giacomozzi, C, Grandone, A, Guzzetti, C, Iughetti, L, Parpagnoli, M, Salerno, M, Street, Me, Tornese, G, Wasniewska, M, Delvecchio, M., Zucchini, S, Lonero, A, Bellone, S, Bozzola, M, Cassio, A, Faienza, Mf, Giacomozzi, C, Grandone, A, Guzzetti, C, Iughetti, L, Parpagnoli, M, Salerno, M, Street, Me, Tornese, G, Wasniewska, M, and Delvecchio, M.

Subjects
growth hormone deficiency, GH, final height, GHD, growth hormone deficiency, final height
Abstract

N/A

Published
2019

27. Death Related to a Congenital Vascular Anomaly of Pulmonary Hamartoma Type: Malpractice or Tragic Fatality?

Author

Marrone, Maricla, Marrone, Laura, Cazzato, Gerardo, Baldassarra, Stefania Lonero, Ingravallo, Giuseppe, and Stellacci, Alessandra

Subjects
FORENSIC pathology, MEDICAL malpractice, BRONCHOSCOPY, HAMARTOMA, DEATH
Abstract

In forensic pathology, apparently straightforward cases can often hide rarities that, if not correctly interpreted, can alter the results of the entire investigation, leading to misinterpretations. This occurs when the investigation is conducted to assess medical malpractice. An unexpected death, with no known apparent cause, is often linked to an underlying disease process of unclear etiological origin whose nature can, unfortunately, be properly investigated only post-mortem. This presentation shows a case study, in which it was possible to reconduct the death of a patient to a natural pathology and not to medical treatment. Here, the authors illustrate a case with a hamartoma developed in chronic inflammatory conditions (bronchiectasis) that was difficult to differentiate from lung cancer due to the inability to perform specific instrumental examinations. The hamartoma, usually benign and identifiable by standard instrumental investigations, in this case, led to the patient’s death precisely during the execution of a bronchoscopy. However, in the absence of a certain cause of death, public opinion unanimously attributes a patient’s disease to medical error. Indeed, a routine practice such as bronchoscopy should not cause death and consequently, the doctor must have made a mistake. Fortunately, the autopsy not only demonstrated the origin of the bleeding but also unveiled the reason for this, as rare congenital lung disease. Fate, one might say. [ABSTRACT FROM AUTHOR]

Published
2021
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29. Insulin pump failures in Italian children with Type 1 diabetes: retrospective 1-year cohort study

Author

Rabbone, I., Minuto, N., Bonfanti, R., Marigliano, M., Cerutti, F., Cherubini, V., d(')Annunzio, G., Frongia, A. P., Iafusco, D., Ignaccolo, G., Lombardo, F., Schiaffini, R., Toni, S., Tumini, S., Zucchini, S., Pistorio, A., Scaramuzza, A. E., Scaramuuzza, A. E., Lera, R., Secco, A., Bobbio, A., Bechaz, M., Piccinno, E., Natale, M. P., Ortolani, F., Zecchino, C., Lonero, A., Maltoni, G., Pasquino, B., Gallo, F., Frongia, P., Ripoli, C., Lo Presti, D., Timpanaro, T., Citriniti, F., Suprani, T., Carinci, S., Cipriano, P., Lazzaro, N., De Donno, V., Gallarotti, F., Lenzi, L., Piccini, B., Vittorio, L., Russo, C., Borea, R., Mamm(`i), F., Bruzzese, M., Ventrici, C., Salzano, G., Frontino, G., Bonura, C., Favalli, V., Scaramuzza, A., Zuccotti, G. V., Ferrari, M., Iughetti, L., Predieri, B., Franzese, A., Mozzillo, E., Buono, P., Confetto, S., Zanfardino, A., Cadario, F., Savastio, S., Fiorito, C., Barbieri, P., Piredda, G., Cardella, F., Ropolo, R., Federico, G., Marchi, B., Benevento, D., Carducci, C., Mancabitti, M. L., Delvecchio, M., Lapolla, R., Gaiero, A., Fichera, G., Ignaccolo, M. G., Tinti, D., Cauvin, V., Franceschi, R., Biagioni, M., Salvatoni, A., Scolari, A., Maffeis, C., Sabbion, A., Arnaldi, C., Tosini, D., Rabbone, I, Minuto, N., Bonfanti, R., Marigliano, M., Cerutti, F., Cherubini, V., D'Annunzio, G., Frongia, A. P., Iafusco, Dario, Ignaccolo, G., Lombardo, F., Schiaffini, R., Toni, S., Tumini, S., Zucchini, S., Pistorio, A., Scaramuzza, A. E., Rabbone, I., Iafusco, D., Lera, R., Secco, A., Bobbio, A., Bechaz, M., Piccinno, E., Natale, M. P., Ortolani, F., Zecchino, C., Lonero, A., Maltoni, G., Pasquino, B., Gallo, F., Frongia, P., Ripoli, C., Lo Presti, D., Timpanaro, T., Citriniti, F., Suprani, T., Carinci, S., Cipriano, P., Lazzaro, N., De Donno, V., Gallarotti, F., Lenzi, L., Piccini, B., Vittorio, L., Russo, C., Borea, R., Mammi, F., Bruzzese, M., Ventrici, C., Salzano, G., Frontino, G., Bonura, C., Favalli, V., Scaramuzza, A., Zuccotti, G. V., Ferrari, M., Iughetti, L., Predieri, B., Franzese, A., Mozzillo, E., Buono, P., Confetto, S., Zanfardino, A., Cadario, F., Savastio, S., Fiorito, C., Barbieri, P., Piredda, G., Cardella, F., Ropolo, R., Federico, G., Marchi, B., Benevento, D., Carducci, C., Mancabitti, M. L., Del Vecchio, M., Lapolla, R., Gaiero, A., Fichera, G., Ignaccolo, M. G., Tinti, D., Cauvin, V., Franceschi, R., Biagioni, M., Salvatoni, A., Scolari, A., Maffeis, C., Sabbion, A., Arnaldi, C., Tosini, D., Rabbone, Minuto, Mammì, F., and Mozzillo, Enza.

Subjects
Blood Glucose, Male, Pediatrics, Adolescent, Blood Glucose Self-Monitoring, Child, Child, Preschool, Diabetes Mellitus, Type 1, Equipment Failure, Female, Humans, Infant, Insulin, Italy, Retrospective Studies, Insulin Infusion Systems, type 1 diabetes, medicine.medical_treatment, Endocrinology, Diabetes and Metabolism, Internal Medicine, Endocrinology, 0302 clinical medicine, Retrospective Studie, Medicine, 030212 general & internal medicine, Diabetes, Diabetology, failure, Diabetes and Metabolism, children and adolescents, insulin pump, Cohort study, Type 1, Human, Insulin pump, medicine.medical_specialty, Disease duration, 030209 endocrinology & metabolism, 03 medical and health sciences, Diabetes mellitus, Diabetes Mellitus, Preschool, Type 1 diabetes, business.industry, Diabetes, Type1, Pump, Insulin, Type1, Retrospective cohort study, Pump, medicine.disease, Surgery, Insulin Infusion System, business
Abstract

Aims Insulin pump failure and/or malfunction requiring replacement have not been thoroughly investigated. This study evaluated pump replacement in children and adolescents with Type 1 diabetes using insulin pump therapy. Methods Data were collected for all participants younger than 19 years, starting insulin pump therapy before 31 December 2013. For each child, age, disease duration, date of insulin pump therapy initiation, insulin pump model, failure/malfunction/replacement yes/no and reason were considered for the year 2013. Results Data were returned by 40 of 43 paediatric centres belonging to the Diabetes Study Group of the Italian Society of Paediatric Endocrinology and Diabetology. In total, 1574 of 11 311 (13.9%) children and adolescents with Type 1 diabetes were using an insulin pump: 29.2% Animas VIBE™, 9.4% Medtronic MiniMed 715/515™, 34.3% Medtronic MiniMed VEO™, 24.3% Accu-Check Spirit Combo™ and 2.8% other models. In 2013, 0.165 insulin pump replacements per patient-year (11.8% due to pump failure/malfunction and 4.7% due to accidental damage) were recorded. Animas VIBE™ (22.1%) and Medtronic MiniMed VEO™ (17.7%) were the most replaced. Conclusions In a large cohort of Italian children and adolescents with Type 1 diabetes, insulin pump failure/malfunction and consequent replacement are aligned with rates previously reported and higher in more sophisticated pump models. This article is protected by copyright. All rights reserved.

Published
2017

31. Evaluation of Beginner Driver Education in Oregon

Author

Robyn D Robertson, Ward G M Vanlaar, Herb M. Simpson, Katherine Wood, Kyla Marcoux, Kathryn M Clinton, Larry Lonero, and Dan Mayhew

Subjects
Engineering, lcsh:Industrial safety. Industrial accident prevention, Applied psychology, Population, teen drivers, Poison control, Computer security, computer.software_genre, Suicide prevention, Occupational safety and health, 03 medical and health sciences, 0302 clinical medicine, 030225 pediatrics, SAFER, 0502 economics and business, Injury prevention, lcsh:T55-55.3, Safety, Risk, Reliability and Quality, education, crashes, 050210 logistics & transportation, education.field_of_study, lcsh:R5-920, evaluation, business.industry, 05 social sciences, beginner drivers, Public Health, Environmental and Occupational Health, Human factors and ergonomics, Small sample, safety effects, driver education (DE), road safety, business, lcsh:Medicine (General), Safety Research, computer
Abstract

Although driver education (DE) is widely accepted as an effective teen driver safety measure and widely available in the United States, Canada and elsewhere, evaluations have generally failed to show that such formal programs actually produce safer drivers. To address the issue of safety effects as part of a larger investigation, two studies were conducted to examine whether the Oregon Department of Transportation (ODOT)-approved DE program was associated with reductions in collisions and convictions. In the first study, DE status among a relatively small sample of teens who completed an online survey was not found to have a significant effect on collisions and convictions. In the second study, of a much larger population of teen drivers, DE status was associated with a lower incidence of collisions and convictions. On balance, this suggests that the safety effects of DE are either neutral, based on the results of the first Oregon study, or cautiously optimistic based on the results of the second study. The implications of these findings are discussed in terms of making improvements in DE that are evidence-based, and the need for further evaluation to establish that improved and new programs meet their safety objectives.

Published
2017

32. VALUE OF THE MEDICAL-LEGAL INVESTIGATION. CORRESPONDENCE OF THE FINDINGS WITH LEGALLY ESTABLISHED REALITY.

Author

Marrone, Maricla, Tarantino, Francesca, Baldassarra, Stefania Lonero, Cazzato, Gerardo, Ezio Santobuono, Vincenzo, Vinci, Francesco, and Stellacci, Alessandra

Subjects
SEXUAL abuse victims, SCIENTIFIC method, RURAL geography
Abstract

In recent years, violence against minors has been the subject of in-depth study in all its historical, social and cultural dimensions, being able to be identified and treated according to rigorous scientific methodologies. The A.A. report the case of an 8-year-old girl, who disappeared in a rural area, found dead a few hours later, on the recommendation of a young man who, despite having admitted to having gone away with the child, had not provided further details. The forensic inspection revealed that the discovery area included an area with burnt undergrowth about 4 meters in diameter. Examination of the external genitalia showed that the hymen was hemorrhagic in the right hemicycle, at 11 o'clock, with laceration of the most superficial layers of the anterior face. In the present case, therefore, despite having limited historical and circumstantial data, it was possible to reconstruct the following damaging dynamic: the little girl, after having gone away with her aggressor, was first the victim of an attempt of sexual violence by the latter and later immobilized by placing a foot on the slender abdomen. She had been covered with brushwood which the attacker had set fire to, thus killing her. This reconstruction of the criminal events, proposed to the investigating magistrate, was confirmed in each passage by the confessions made by the murderer, during the various degrees of trial, so much so that the final sentence reported in full the specific steps of the medico-legal relationship. [ABSTRACT FROM AUTHOR]

Published
2021
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33. A Cluster of Cryptic Plasmodium falciparum Malaria in African Migrants in Southern Italy, October 2017.

Author

Brindicci, Gaetano, Santoro, Carmen Rita, Loconsole, Daniela, Martinelli, Domenico, Prato, Rosa, Lonero, Gaetano, Loperfido, Pietro, Buccoliero, Giovanni Battista, and Chironna, Maria

Subjects
MALARIA, PLASMODIUM falciparum, AFRICANS, MIGRANT agricultural workers, PLASMODIUM vivax
Abstract

Background: Italy was declared malaria free by the World Health Organization in 1970. Despite this, nonimport malaria cases are on the increase in Italy and throughout the Mediterranean area. In Italy, in the period between 2011 and 2015, seven cases of locally acquired malaria have been reported, including one introduced case of Plasmodium vivax; moreover, the last certain case of introduced malaria (by P. vivax) has been reported in Tuscany in 1997. No case of introduced malaria from Plasmodium falciparum has been reported in Italy since 1970. Case Presentation: A cluster of four cryptic P. falciparum malaria cases were ascertained in migrant farm workers (three from Morocco and one from Sudan) in Apulia (southern Italy) with clinical onset between September 20 and 27, 2017. None of the patients reported a history of a recent trip to malaria-endemic areas or hospitalization or other risk factors. Typing of malaria was also confirmed using molecular biology methods in two different laboratories. There were no cases of severe malaria in our four patients, and only one in need of transfusion. All patients were discharged cured after being treated with mefloquine due to the unavailability of other antimalarials. Conclusions: In recent years, numerous reports of locally acquired malaria have been made in southern Europe. The cases described in this article represent the first cluster of malaria caused by P. falciparum in Europe. Today, clinical presentation in the diagnosis of malaria is more important than ever, since epidemiological criterion cannot be considered unfailing. The mode of transmission has not been proven and further biological and entomological studies are necessary to define our case as cryptic or confirm the presence of mosquitoes capable of transmitting P. falciparum and/or the capacity of Anopheles labranchiae, An. superpictus, or An. plumbeus to transmit it on Italian territory. [ABSTRACT FROM AUTHOR]

Published
2020
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35. Metabolic Outcomes, Bone Health, and Risk of Polycystic Ovary Syndrome in Girls with Idiopathic Central Precocious Puberty Treated with Gonadotropin-Releasing Hormone Analogues

Author

Maurizio Delvecchio, Luciano Cavallo, Domenico Corica, Alberto Gaeta, Antonella Lonero, Malgorzata Wasniewska, Maria Rosa Velletri, Filippo De Luca, A Acquafredda, Paola Suavo Bulzis, Maria Felicia Faienza, and Giacomina Brunetti

Subjects
medicine.medical_specialty, business.industry, Endocrinology, Diabetes and Metabolism, Central precocious puberty, 030209 endocrinology & metabolism, Gonadotropin-releasing hormone, medicine.disease, Polycystic ovary, Bone health, 03 medical and health sciences, 0302 clinical medicine, Endocrinology, Insulin resistance, Bone status, Gonadotropin-releasing hormone analogue treatment, Idiopathic central precocious puberty, Polycystic ovary syndrome, Pediatrics, Perinatology and Child Health, Internal medicine, medicine, 030212 general & internal medicine, business, Hormone
Abstract

Background/Aims: Gonadotropin-releasing hormone analogues (GnRHa) represent the gold standard treatment for central precocious puberty (CPP). We aimed to assess the effects of GnRHa treatment on metabolic outcomes, bone status, and polycystic ovary syndrome (PCOS) prevalence in young girls with idiopathic CPP (ICPP). Methods: We enrolled 94 ICPP girls who were at least 2 years after menarche and had already attained adult height at the time of the study: 56 previously treated with depot triptorelin (3.4 ± 0.6 years) and 38 untreated. Auxological parameters, lipid profile, homeostatic model assessment of insulin resistance (HOMA-IR), bone state, and prevalence of PCOS were assessed. Results: The 2 groups were similar for body mass index (BMI) and waist circumference. HOMA-IR, dehydroepi­androsterone sulfate, and Δ4-androstenedione were higher in the treated than in the untreated subjects (p < 0.001). Significant differences were found for amplitude-dependent speed of sound (p < 0.03) and bone transmission time z-scores (p < 0.01). The prevalence of PCOS was higher in the treated than in the untreated subjects (p < 0.04). Conclusion: GnRHa therapy is associated with hyperandrogenism and an increase in insulin resistance and PCOS prevalence, but not with increased BMI or lipid profile alterations. Long-term evaluations at the time of expected peak bone mass achievement are needed to understand the persistent or transient nature of subtle bone abnormalities.

Published
2017

36. The molecular characterization of a depurinated trial DNA sample can be a model to understand the reliability of the results in forensic genetics

Author

Gregorio Seidita, Peter M. Schneider, Paola Pitacco, Silvia Corato, Eugenia Carnevali, Carla Vecchiotti, Pierangela Grignani, Solange Sorçaburu-Cigliero, Milena Alù, Anna Barbaro, Stefania Turrina, Andrea Verzeletti, Francesco De Stefano, Francesca Scarnicci, Laura Plizza, Stefania Lonero Baldassarra, Matteo Fabbri, Angel Carracedo, Carlo Previderè, Ranieri Domenici, Nicoletta Resta, Paolo Vatta, L. Casarino, Chiara Turchi, Lara Consoloni, Lucia Trizzino, Carlo Robino, Ugo Ricci, Vanessa Nicolin, Paolo Fattorini, Marco Moratti, Giorgio Marrubini, Luca Salvaderi, Emiliano Giardina, Susi Pelotti, Andrea Piccinini, Fattorini, Paolo, Previderè, Carlo, Sorçaburu-Cigliero, Solange, Marrubini, Giorgio, Alù, Milena, Barbaro, Anna M., Carnevali, Eugenia, Carracedo, Angel, Casarino, Lucia, Consoloni, Lara, Corato, Silvia, Domenici, Ranieri, Fabbri, Matteo, Giardina, Emiliano, Grignani, Pierangela, Baldassarra, Stefania Lonero, Moratti, Marco, Nicolin, Vanessa, Pelotti, Susi, Piccinini, Andrea, Pitacco, Paola, Plizza, Laura, Resta, Nicoletta, Ricci, Ugo, Robino, Carlo, Salvaderi, Luca, Scarnicci, Francesca, Schneider, Peter M., Seidita, Gregorio, Trizzino, Lucia, Turchi, Chiara, Turrina, Stefania, Vatta, Paolo, Vecchiotti, Carla, Verzeletti, Andrea, De Stefano, Francesco, Previderè, C, Sorçaburu Cigliero, S, Marrubini, G, Alù, M, Barbaro, Am, Carnevali, E, Carracedo, A, Casarino, L, Consoloni, L, Corato, S, Domenici, R, Fabbri, M, Giardina, E, Grignani, P, Baldassarra, Sl, Moratti, M, Pelotti, S, Piccinini, A, Pitacco, P, Plizza, L, Resta, N, Ricci, U, Robino, C, Salvaderi, L, Scarnicci, F, Schneider, Pm, Seidita, G, Trizzino, L, Turchi, C, Turrina, S, Vatta, P, Vecchiotti, C, Verzeletti, A, De Stefano, F., Fattorini, P., Previderè, C., Sorçaburu-Cigliero, S., Marrubini, G., Alù, M., Barbaro, A., Carnevali, E., Carracedo, A., Casarino, L., Consoloni, L., Corato, S., Domenici, R., Fabbri, M., Giardina, E., Grignani, P., Baldassarra, S., Moratti, M., Nicolin, V., Pelotti, S., Piccinini, A., Pitacco, P., Plizza, L., Resta, N., Ricci, U., Robino, C., Salvaderi, L., Scarnicci, F., Schneider, P., Seidita, G., Trizzino, L., Turchi, C., Turrina, S., Vatta, P., Vecchiotti, C., and Verzeletti, A.

Subjects
DNA depurination, Forensic genetics, PCR fidelity, STR typing, Biochemistry, Clinical Biochemistry, Genotyping Techniques, DNA damage, Sample (material), Reproducibility of Result, Biology, Polymerase Chain Reaction, NO, Analytical Chemistry, law.invention, forensic genetics, Settore MED/43 - Medicina Legale, law, Settore BIO/13 - Biologia Applicata, Genotype, Humans, Polymerase chain reaction, Protocol (science), Genetics, Medicine (all), Reproducibility of Results, Forensic genetic, DNA, Amplicon, DNA Fingerprinting, Settore BIO/18 - Genetica, DNA depurination, Forensic genetics, PCR fidelity, STR typing, DNA profiling, Settore MED/03 - Genetica Medica, Microsatellite Repeat, Genotyping Technique, Microsatellite Repeats, Human
Abstract

The role of DNA damage in PCR processivity/fidelity is a relevant topic in molecular investigation of aged/forensic samples. In order to reproduce one of the most common lesions occurring in postmortem tissues, a new protocol based on aqueous hydrolysis of the DNA was developed in vitro. Twenty-five forensic laboratories were then provided with 3.0 μg of a trial sample (TS) exhibiting, in mean, the loss of 1 base of 20, and a molecular weight below 300 bp. Each participating laboratory could freely choose any combination of methods, leading to the quantification and to the definition of the STR profile of the TS, through the documentation of each step of the analytical approaches selected. The results of the TS quantification by qPCR showed significant differences in the amount of DNA recorded by the participating laboratories using different commercial kits. These data show that only DNA quantification "relative" to the used kit (probe) is possible, being the "absolute" amount of DNA inversely related to the length of the target region (r(2) = 0.891). In addition, our results indicate that the absence of a shared stable and certified reference quantitative standard is also likely involved. STR profiling was carried out selecting five different commercial kits and amplifying the TS for a total number of 212 multiplex PCRs, thus representing an interesting overview of the different analytical protocols used by the participating laboratories. Nine laboratories decided to characterize the TS using a single kit, with a number of amplifications varying from 2 to 12, obtaining only partial STR profiles. Most of the participants determined partial or full profiles using a combination of two or more kits, and a number of amplifications varying from 2 to 27. The performance of each laboratory was described in terms of number of correctly characterized loci, dropped-out markers, unreliable genotypes, and incorrect results. The incidence of unreliable and incorrect genotypes was found to be higher for participants carrying out a limited number of amplifications, insufficient to define the correct genotypes from damaged DNA samples such as the TS. Finally, from a dataset containing about 4500 amplicons, the frequency of PCR artifacts (allele dropout, allele drop-in, and allelic imbalance) was calculated for each kit showing that the new chemistry of the kits is not able to overcome the concern of template-related factors. The results of this collaborative exercise emphasize the advantages of using a standardized degraded DNA sample in the definition of which analytical parameters are critical for the outcome of the STR profiles.

Published
2014

37. Analysis of Neolithic human remains discovered in southern Italy

Author

De Donno A, Santoro V, Di Fazio A, Corrado S, Urso D, Lonero Baldassarra S, Introna F., DI NUNNO, Nunzio, De Donno, A, Santoro, V, Di Fazio, A, Corrado, S, Urso, D, Lonero Baldassarra, S, DI NUNNO, Nunzio, and Introna, F.

Subjects
Ancient DNA, Forensic Sciences, Neolithic
Abstract

The authors describe the discovery of the remains of two unidentified skeletonised individuals in a small town located in southern Italy. The bodies were discovered while workers were preparing to lay an oil pipeline. The two individuals were found at a depth of 2 meters, and in very close proximity to one other. The recovery process of the skeletonised remains and their related findings, carried out by a team of forensic anthropologists and archaeologists, is described here. Archaeological examination determined that the remains date back to the 4th millennium B.C. Forensic anthropological and odontological examinations were performed to determine the biological profile of the skeletal remains by estimation of age and height, as well as the determination of sex. Age determination was performed by the Kerley and Ubelaker (1978, Revision in the microscopic method of estimating age at death in human cortical bone. Am. J. Phys. Anthropol., 49, 545–546) histological method using a 1 mm thick piece of bone tissue taken from the diaphyses of femur in both individuals. Dental age was estimated by examining root transparency. In order to determine the height of the individuals, various research methods based on the dimensional values of particular skeletal structures were applied. DNA analysis showed genotype differences of all the systems as compared to the haplotypes of present day subjects.

Published
2010

38. Adherence issues related to sublingual immunotherapy as perceived by allergists

Author

Scurati, S., Frati, F., Passalacqua, G., Puccinelli, P., Hilaire, C., Incorvaia, C., D Avino, G., Comi, R., Lo Schiavo, M., Pezzuto, F., Montera, C., Pio, A., Teresa Ielpo, M., Cellini, F., Vicentini, L., Pecorari, R., Aresu, T., Capra, L., Benedictis, E., Bombi, C., Zauli, D., Vanzi, A., Alberto Paltrinieri, C., Bondioli, A., Paletta, I., Ventura, D., Mei, F., Paolini, F., Colangelo, C., Cavallucci, E., Cucinelli, F., Tinari, R., Ermini, G., Beltrami, V., Novembre, E., Begliomini, C., Marchese, E., Solito, E., Ammannati, V., Molino, G., Galli, E., Baldassini, M., Di Michele, L., Calvani, M., Gidaro, M., Venuti, A., Li Bianchi, E., Benassi, F., Pocobelli, D., Zangari, P., Rocco, M. G., Lo Vecchio, A., Pingitore, G., Grimaldi, O., Schiavino, D., Perrone, N., Antonietta Frieri, M., Di Rienzo, V., Tripodi, S., Scarpa, A., Tomsic, M., Bonaguro, R., Enrico Senna, G., Sirena, A., Turatello, F., Crescioli, S., Favero, E., Billeri, L., Chieco Bianchi, F., Gemignani, C., Zanforlin, M., Angiola Crivellaro, M., Hendrick, B., Maltauro, A., Masieri, S., Elisabetta Conte, M., Fama, M., Pozzan, M., Bonadonna, P., Casanova, S., Vallerani, E., Schiappoli, M., Borghesan, F., Giro, G., Casotto, S., Berardino, L., Zanoni, G., Ariano, R., Aquilina, R., Pellegrino, R., Marsico, P., Del Giudice, A., Narzisi, G., Tomaselli, V., Fornaca, G., Favro, M., Loperfido, B., Gallo, C., Buffoni, S., Gani, F., Raviolo, P., Faggionato, S., Truffelli, T., Vivalda, L., Albano, M., Enzo Rossi, R., Lattuada, G., Bona, F., Quaglio, L., Chiesa, A., Trapani, M., Seminara, R., Cucchi, B., Oderda, S., Borio, G., Galeasso, G., Garbaccio, P., Marco, A., Marengo, F., Cadario, G., Manzoni, S., Vinay, C., Curcio, A., Silvestri, A., Peduto, A., Riario-Sforza, G. G., Maria Forgnone, A., Barocelli, P., Tartaglia, N., Feyles, G., Giacone, A., Ricca, V., Guida, G., Nebiolo, F., Bommarito, L., Heffler, E., Vietti, F., Galimberti, M., Savi, E., Pappacoda, A., Bottero, P., Porcu, S., Felice, G., Berra, D., Francesca Spina, M., Pravettoni, V., Calamari, A. M., Varin, E., Iemoli, E., Lietti, D., Ghiglioni, D., Alessandro Fiocchi, Tosi, A., Poppa, M., Caviglia, A., Restuccia, M., Russello, M., Alciato, P., Manzotti, G., Ranghino, E., Luraschi, G., Rapetti, A., Rivolta, F., Allegri, F., Terracciano, L., Agostinis, F., Paolo Piras, P., Ronchi, G., Gaspardini, G., Caria, V., Tolu, F., Fantasia, D., Carta, P., Moraschini, A., Quilleri, R., Santelli, A., Prandini, P., Del Giudice, G., Apollonio, A., Bonazza, L., Teresa Franzini, M., Branchi, S., Zanca, M., Rinaldi, S., Catelli, L., Zanoletti, T., Cosentino, C., Della Torre, F., Cremonte, L., Musazzi, D., Suli, C., Rivolta, L., Ottolenghi, A., Marino, G., Sterza, G., Sambugaro, R., Orlandini, A., Minale, P., Voltolini, S., Bignardi, D., Omodeo, P., Tiri, A., Milani, S., Ronchi, B., Licardi, G., Bruni, P., Scibilia, J., Schroeder, J., Crosti, F., Maltagliati, A., Alesina, M. R., Mosca, M., Leone, G., Napolitano, G., Di Gruttola, G., Scala, G., Mascio, S., Valente, A., Marchetiello, I., Catello, R., Gazulli, A., Del Prete, A., Varricchio, A. M., Carbone, A., Forestieri, A., Stillitano, M., Leonetti, L., Tirroni, E., Castellano, F., Abbagnara, F., Romano, F., Levanti, C., Cilia, M., Longo, R., Ferrari, A., Merenda, R., Di Ponti, A., Guercio, E., Surace, L., Ammendola, G., Tansella, F., Peccarisi, L., Stragapede, L., Minenna, M., Granato, M., Fuiano, N., Pannofino, A., Ciuffreda, S., Giannotta, A., Morero, G., D Oronzio, L., Taddeo, G., Nettis, E., Cinquepalmi, G., Lamanna, C., Mastrandrea, F., Minelli, M., Salamino, F., Muratore, L., Latorre, F., Quarta, C., Ventura, M., D Ippolito, G., Giannoccaro, F., Dambra, P., Pinto, L., Triggiani, M., Munno, G., Manfredi, G., Lonero, G., Damiano, V., Errico, G., Di Leo, E., Manzari, F., Spagna, V., Arsieni, A., Matarrese, A., Mazzarella, G., Scarcia, G., Scarano, R., Ferrannini, A., Pastore, A., Maionchi, P., Filannino, L., Tria, M., Giuliano, G., Damiani, E., Scichilone, N., Marchese, M., Lucania, A., Marino, M., Strazzeri, L., Tumminello, S., Vitale, G. I., Gulotta, S., Gragotto, G., Zambito, M., Greco, D., Valenti, G., Licitra, G., Cannata, E., Filpi, R., Contraffatto, M., Sichili, S., Randazzo, S., Scarantino, G., Lo Porto, B., Pavone, F., Di Bartolo, C., Paternò, A., Rapisarda, F., Laudani, E., Leonardi, S., Padua, V., Cabibbo, G., Marino Guzzardi, G., Deluca, F., Agozzino, C., Pettinato, R., Ghini, M., Scurati S., Frati F., Passalacqua G., Puccinelli P., Hilaire C., Incorvaia C., D'Avino G., Comi R., Lo Schiavo M., Pezzuto F., Montera C., Pio A., Teresa Ielpo M., Cellini F., Vicentini L., Pecorari R., Aresu T., Capra L., De Benedictis E., Bombi C., Zauli D., Vanzi A., Alberto Paltrinieri C., Bondioli A., Paletta I., Ventura D., Mei F., Paolini F., Colangelo C., Cavallucci E., Cucinelli F., Tinari R., Ermini G., Beltrami V., Novembre E., Begliomini C., Marchese E., Solito E., Ammannati V., Molino G., Galli E., Baldassini M., Di Michele L., Calvani M., Gidaro M., Venuti A., Li Bianchi E., Benassi F., Pocobelli D., Zangari P., De Rocco M.G., Lo Vecchio A., Pingitore G., Grimaldi O., Schiavino D., Perrone N., Antonietta Frieri M., Di Rienzo V., Tripodi S., Scarpa A., Tomsic M., Bonaguro R., Enrico Senna G., Sirena A., Turatello F., Crescioli S., Favero E., Billeri L., Chieco Bianchi F., Gemignani C., Zanforlin M., Angiola Crivellaro M., Hendrick B., Maltauro A., Masieri S., Elisabetta Conte M., Fama M., Pozzan M., Bonadonna P., Casanova S., Vallerani E., Schiappoli M., Borghesan F., Giro G., Casotto S., Berardino L., Zanoni G., Ariano R., Aquilina R., Pellegrino R., Marsico P., Del Giudice A., Narzisi G., Tomaselli V., Fornaca G., Favro M., Loperfido B., Gallo C., Buffoni S., Gani F., Raviolo P., Faggionato S., Truffelli T., Vivalda L., Albano M., Enzo Rossi R., Lattuada G., Bona F., Quaglio L., Chiesa A., Trapani M., Seminara R., Cucchi B., Oderda S., Borio G., Galeasso G., Garbaccio P., De Marco A., Marengo F., Cadario G., Manzoni S., Vinay C., Curcio A., Silvestri A., Peduto A., Riario-Sforza G.G., Maria Forgnone A., Barocelli P., Tartaglia N., Feyles G., Giacone A., Ricca V., Guida G., Nebiolo F., Bommarito L., Heffler E., Vietti F., Galimberti M., Savi E., Pappacoda A., Bottero P., Porcu S., Felice G., Berra D., Francesca Spina M., Pravettoni V., Calamari A.M., Varin E., Iemoli E., Lietti D., Ghiglioni D., Fiocchi A., Tosi A., Poppa M., Caviglia A., Restuccia M., Russello M., Alciato P., Manzotti G., Ranghino E., Luraschi G., Rapetti A., Rivolta F., Allegri F., Terracciano L., Agostinis F., Paolo Piras P., Ronchi G., Gaspardini G., Caria V., Tolu F., Fantasia D., Carta P., Moraschini A., Quilleri R., Santelli A., Prandini P., Del Giudice G., Apollonio A., Bonazza L., Teresa Franzini M., Branchi S., Zanca M., Rinaldi S., Catelli L., Zanoletti T., Cosentino C., Della Torre F., Cremonte L., Musazzi D., Suli C., Rivolta L., Ottolenghi A., Marino G., Sterza G., Sambugaro R., Orlandini A., Minale P., Voltolini S., Bignardi D., Omodeo P., Tiri A., Milani S., Ronchi B., Licardi G., Bruni P., Scibilia J., Schroeder J., Crosti F., Maltagliati A., Alesina M.R., Mosca M., Leone G., Napolitano G., Di Gruttola G., Scala G., Mascio S., Valente A., Marchetiello I., Catello R., Gazulli A., Del Prete A., Varricchio A.M., Carbone A., Forestieri A., Stillitano M., Leonetti L., Tirroni E., Castellano F., Abbagnara F., Romano F., Levanti C., Cilia M., Longo R., Ferrari A., Merenda R., Di Ponti A., Guercio E., Surace L., Ammendola G., Tansella F., Peccarisi L., Stragapede L., Minenna M., Granato M., Fuiano N., Pannofino A., Ciuffreda S., Giannotta A., Morero G., D'Oronzio L., Taddeo G., Nettis E., Cinquepalmi G., Lamanna C., Mastrandrea F., Minelli M., Salamino F., Muratore L., Latorre F., Quarta C., Ventura M., D'Ippolito G., Giannoccaro F., Dambra P., Pinto L., Triggiani M., Munno G., Manfredi G., Lonero G., Damiano V., Errico G., Di Leo E., Manzari F., Spagna V., Arsieni A., Matarrese A., Mazzarella G., Scarcia G., Scarano R., Ferrannini A., Pastore A., Maionchi P., Filannino L., Tria M., Giuliano G., Damiani E., Scichilone N., Marchese M., Lucania A., Marino M., Strazzeri L., Tumminello S., Vitale G.I., Gulotta S., Gragotto G., Zambito M., Greco D., Valenti G., Licitra G., Cannata E., Filpi R., Contraffatto M., Sichili S., Randazzo S., Scarantino G., Lo Porto B., Pavone F., Di Bartolo C., Paterno A., Rapisarda F., Laudani E., Leonardi S., Padua V., Cabibbo G., Marino Guzzardi G., Deluca F., Agozzino C., Pettinato R., Ghini M., Scurati S, Frati F, Passalacqua G, Puccinelli P, Hilaire C, Incorvaia I, D'Avino G, Comi R, Lo Schiavio M, Pezzuto F, Montera C, Pio A, Ielpo MT, Cellini F, Vicentini L, Pecorari R, Aresu T, Capra L, De Benedictis E, Bombi C, Zauli D, and et al

Subjects
medicine.medical_specialty, Pathology, genetic structures, efficacy, Alternative medicine, Medicine (miscellaneous), Adherence, Cost, Efficacy, Side effects, Sublingual immunotherapy, Settore MED/10 - Malattie Dell'Apparato Respiratorio, sublingual immunotherapy, ALLERGEN, cost, medicine, Subcutaneous immunotherapy, Sublingual immunotherapy, adherence, Clinical efficacy, Intensive care medicine, Pharmacology, Toxicology and Pharmaceutics (miscellaneous), sublingual immunoterapy, Original Research, Asthma, AEROALLERGENS, side effects, business.industry, Health Policy, medicine.disease, Slit, eye diseases, Clinical trial, Patient Preference and Adherence, immunotherapy, sense organs, Allergists, ADHERENCE TO TREATMENT, business, Social Sciences (miscellaneous)
Abstract

Silvia Scurati1, Franco Frati1, Gianni Passalacqua2, Paola Puccinelli1, Cecile Hilaire1, Cristoforo Incorvaia3, Italian Study Group on SLIT Compliance 1Scientific and Medical Department, Stallergenes, Milan, Italy; 2Allergy and Respiratory Diseases, Department of Internal Medicine, Genoa; 3Allergy/Pulmonary Rehabilitation, ICP Hospital, Milan, ItalyObjectives: Sublingual immunotherapy (SLIT) is a viable alternative to subcutaneous immunotherapy to treat allergic rhinitis and asthma, and is widely used in clinical practice in many European countries. The clinical efficacy of SLIT has been established in a number of clinical trials and meta-analyses. However, because SLIT is self-administered by patients without medical supervision, the degree of patient adherence with treatment is still a concern. The objective of this study was to evaluate the perception by allergists of issues related to SLIT adherence.Methods: We performed a questionnaire-based survey of 296 Italian allergists, based on the adherence issues known from previous studies. The perception of importance of each item was assessed by a VAS scale ranging from 0 to 10.Results: Patient perception of clinical efficacy was considered the most important factor (ranked 1 by 54% of allergists), followed by the possibility of reimbursement (ranked 1 by 34%), and by the absence of side effects (ranked 1 by 21%). Patient education, regular follow-up, and ease of use of SLIT were ranked first by less than 20% of allergists.Conclusion: These findings indicate that clinical efficacy, cost, and side effects are perceived as the major issues influencing patient adherence to SLIT, and that further improvement of adherence is likely to be achieved by improving the patient information provided by prescribers.Keywords: adherence, sublingual immunotherapy, efficacy, cost, side effects

Published
2010

39. DNA extracration and Anthropological Aspects from 6th to 7th Century A.D. Bone Remains

Author

DI NUNNO, Nunzio, VITO SCATTARELLA, SANDRO SUBLIMI SAPONETTI, PATRIZIA EMANUEL, STEFANIA LONERO BALDASSARRA, COSIMO DI NUNNO, DI NUNNO, Nunzio, Vito, Scattarella, SANDRO SUBLIMI, Saponetti, Patrizia, Emanuel, STEFANIA LONERO, Baldassarra, and COSIMO DI, Nunno

Abstract

In the archeological site of the early Christian Episcopal complex of Saint Peter, in Canosa di Puglia (Bari, Italy), during the operations of archaeological excavations, tombs were discovered. They were dated between the sixth and seventh centuries AD with carbon 14 methodology. Five skeletons were found in the 5 tombs: 28A: male individual, 43 years old. The height was 170 cm; the biomass was 65.7 kg. The analysis of the bones indicated several noteworthy pathologies, such as a number of hypoplasia lines of the enamel, the presence of Schmorl hernias on the first 2 lumbar vertebrae, and the outcome of subacromial impingement syndrome. 28E was a male individual, with a biologic age of death of between 44 and 60 years. The height was 177 cm. He had a posttraumatic fracture callus of the medial third of the clavicle, with an oblique fracture rima. 29B was a female individual, 44–49 years old. The height was 158.8 cm; the biomass was 64.8 kg. There was Wells bursitis on the ischial tuberosity on both sides. 29E was a male individual, 45–50 years old. The height was 169.47 cm; the biomass was 70.8 kg. The third and the fourth vertebrae showed Baastrup syndrome (compression of the vertebral spine). There were radiologic signs of deformity on the higher edge of the acetabula and results of frequent sprains of the ankles. 31A was a male individual, 47–54 years old. The height was 178.65 cm; the biomass was 81 kg. The vertebral index showed a heavy overloading in the thoracic lumbar region. There were bony formations under the periosteum on both on the higher and medium facets of the first metatarsus and on the higher and lateral facets of the fifth metatarsus on both sides. As the topography indicates, these small ossifications coincided with the contact points between the back of the foot and parts of the upper shoe. From the osseous remains, in particular from the teeth (central incisors), the DNA was extracted and typed to identify potential family ties among all the subjects. The extraction technique used came from the DNA Promega technique, partially modified by the authors. Stay times of the sample in the extraction buffer were increased.

Published
2005

40. The molecular characterisation of depurinated trial DNA sample can be a model to understand the reliability of the results in forensic genetics

Author

Fattorini, P., Previdere`, C., Sorçaburu Cigliero, S., Marrubini, G., Alù, M., Barbaro, A., Carnevali, E., Carracedo, A., Casarino, L., Consoloni, L., Corato, S., Domenici, R., Fabbri, M., Giardina, E., Grignani, P., Lonero Baldassarra, S., Moratti, M., Pelotti, S., Piccinini, A., Pitacco, P., Plizza, L., Resta, N., Ricci, U., Robino, C., Salvaderi, L., Scarnicci, F., Schneider, P. M., Seidita, G., Trizzino, L., Turchi, C., Turrina, Stefania, Vatta, P., Vecchiotti, C., Verzeletti, A., and De Stefano, F.

Subjects
DNA depurination, Forensic genetics, PCR fidelity, STR typing
Published
2014

41. Allele frequencies of the new European Standard Set (ESS) loci in a population of Apulia (Southern Italy)

Author

Alessandro Dell'Erba, Marilidia Piglionica, S. Lonero Baldassarra, Emiliano Giardina, Nicoletta Resta, and L. Tonino Marsella

Subjects
miniSTRs, Concordance, Population, Locus (genetics), Biology, Genetic analysis, Polymerase Chain Reaction, Pathology and Forensic Medicine, law.invention, New European Standard Set (ESS), Gene Frequency, Settore MED/43 - Medicina Legale, law, Genotype, Apulian population, Genetics, European standard, Humans, education, Allele frequency, Polymerase chain reaction, education.field_of_study, Europe, Italy, Settore MED/03 - Genetica Medica
Abstract

Allele frequencies of five miniSTRs loci (D1S1656, D2S441, D12S391, D10S1248 and D22S1045) included in the new European Standard Set (ESS) were calculated from a sample of 150 unrelated individuals from Apulia, a Region of Southern Italy. Two different PCR Amplification Kits were used, in order to evaluate the concordance of the genotypes. The results obtained with the two kits showed no differences in all genotype profiles. No deviation from Hardy–Weinberg expectations was detected at either locus. Moreover genetic analysis using Fst estimation showed no evidence for differentiation at the five new loci between Apulia and Italian populations. The high levels of polymorphisms of the analyzed markers in the Apulian population allow to confirm that these markers are useful tools in paternity and forensic analysis from degraded DNA samples.

Published
2013

42. A narrative review of the technical standards for extracorporeal life support devices (pumps and oxygenators) in Europe.

Author

Di Nardo, Matteo, Vercaemst, Leen, Swol, Justyna, Barret, Nicholas, Taccone, Fabio S., Malfertheiner, Maximilian V., Broman, Lars M., Pappalardo, Federico, Belohlavek, Jan, Mueller, Thomas, Lorusso, Roberto, Lonero, Margherita, and Belliato, Mirko

Subjects
EXTRACORPOREAL membrane oxygenation, DRUG infusion pumps, LIFE support systems in critical care, OXYGENATORS, STANDARDS, EQUIPMENT & supplies
Abstract

This review summarizes the European rules to control the market when introducing new products. In particular, it shows all the steps to achieve the European Conformity (CE Mark), a certification that all new medical products must achieve before being used in Europe. Extracorporeal membrane oxygenation (ECMO) devices are exposed to the same procedures. Hereby, we present some regulatory issues regarding pumps and oxygenators, providing technical details as released by the manufacturers on their websites and information charts. [ABSTRACT FROM AUTHOR]

Published
2018
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43. The first five years of neonatal and pediatric transports on extracorporeal membrane oxygenation in the center and south of Italy: The pediatric branch of the Italian “Rete Respira” network.

Author

Di Nardo, Matteo, Lonero, Margherita, Pasotti, Elisabetta, Cancani, Federica, Perrotta, Daniela, Cecchetti, Corrado, Stoppa, Francesca, Pirozzi, Nicola, La Salvia, Ondina, Nicolini, Antonella, Amodeo, Antonio, Patroniti, Nicolo’, and Pesenti, Antonio

Subjects
*CHI-squared test, *EXTRACORPOREAL membrane oxygenation, *HOSPITAL admission & discharge, *PATIENT safety, *PROBABILITY theory, *SURVIVAL analysis (Biometry), *TREATMENT effectiveness, *TRANSPORTATION of patients, *DATA analysis software, *DESCRIPTIVE statistics
Abstract

Introduction: Neonatal and pediatric ECMO is a high-risk procedure that should be performed only in expert centers. Children who are eligible for ECMO and are managed in hospitals without ECMO capabilities should be referred to the closest ECMO center before the severity of illness precludes safe conventional transport. When the clinical situation precludes safe conventional transport, ECMO should be provided on site with the patient transported on ECMO. Methods: We retrospectively reviewed our institutional database of all ECMO transports for neonatal and pediatric respiratory failure from February 2013 to February 2018. Results: Over the last 5 years, we provided 24 transports covering all requests from the center and south of Italy except for the islands. Of these transports, 20 were performed on ECMO and 4 without ECMO. No patient died during transportation. Five complications were reported only during the ECMO transports, and all of these were managed without compromising the patient’s safety. The preferred modes of transport were by ambulance (70%) and ambulance transported into the fixed wing aircraft (30%) for longer national distances. The survival to hospital discharge of the patients transported with ECMO was 75% among the neonatal transports and 83.3% among the pediatric transports. The survival to hospital discharge of the four patients transported without ECMO was 100% for both neonates and children. Conclusions: Neonatal and pediatric ECMO transports can be safely performed with a dedicated team that maintains stringent adherence to well-designed management protocols. [ABSTRACT FROM AUTHOR]

Published
2018
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44. Population data for 17 Y-chromosome STRs in a sample from Apulia (Southern Italy)

Author

Emiliano Giardina, S. Lonero Baldassarra, Alessandro Stella, Paola Frati, Francesco Domenico D'Ovidio, Nicoletta Resta, Alessandro Dell'Erba, Gennaro Maria Lenato, and Marilidia Piglionica

Subjects
Veterinary medicine, Population data, Population, Locus (genetics), Biology, Y chromosome, Analysis of molecular variance, y-strs, population data, italy, Pathology and Forensic Medicine, Gene Frequency, Genetics, Humans, Allele, education, Allele frequency, education.field_of_study, Chromosomes, Human, Y, Haplotype, Y-STRs, Genetics, Population, Genetic distance, Haplotypes, Italy, Settore MED/03 - Genetica Medica, Microsatellite Repeats
Abstract

The 17 Y-STR loci included in the AmpFLSTR Yfiler PCR Amplification Kit were analyzed in 98 unrelated healthy males from Apulia (Southern Italy). A total of 97 different haplotypes were identified, of which 96 haplotypes were unique and 1 occurred twice. Allele frequencies for each Y-STR locus in pooled sample and estimated value of gene diversity (GD) were evaluated. The lowest value of GD was observed for DYS392 (0.126) and the highest one (0.936) for DYS385. The HD (haplotype diversity) for the studied Y-STR set showed a value of 0.9994, with an HMP (haplotype match probability) value of 0.0006, while the overall DC was 98.98%. Microvariant alleles were found for the DYS458 and DYS385 markers and sequenced. Furthermore, Φst-based genetic distance computation and pair-wise analysis of molecular variance (AMOVA) test were carried out. When comparing our population with the Apulia sample previously investigated, the AMOVA analysis detected no evidence for significant differentiation. The comparison with all Italian populations submitted to the YHRD website showed no relevant differences with all Southern Italian populations (San Giorgio La Molara, Belvedere, Trapani and Catania) and significant genetic deviation with all Northern Italian populations (Udine, Biella, La Spezia, Modena, Ravenna, Marche and North Sardinia). Moreover, the other populations and meta-populations belonging to the whole Mediterranean area (Croatia, Macedonia, Albania, Greece, Turkey, Israel, Libya, Tunisia, Algeria, Morocco and Spain) were different from our Apulia sample. The data were submitted to YHRD.

Published
2013

46. SMARTER CARS NEED SMARTER DRIVERS

Author

SHARFMAN, WILLIAM L. and LONERO, LAWRENCE P.

Subjects
Automobile industry, Business
Abstract

One of our judges for the Automotive News PACE Awards recently expressed frustration that drivers are ill-prepared to use sophisticated, innovative vehicle technology. For example, they defeat the smart airbag [...]

Published
1999

47. Shocking scenario

Author

Lonero, Larry

Subjects
Sports and fitness
Abstract

In his article on shore power ('Power Play,' January) Tom Hale recommends putting dielectric grease and spray on electrical connections--this seems to me a poor idea, since they are themselves [...]

Published
2016

49. Metabolic Outcomes, Bone Health, and Risk of Polycystic Ovary Syndrome in Girls with Idiopathic Central Precocious Puberty Treated with Gonadotropin-Releasing Hormone Analogues.

Author

Faienza, Maria Felicia, Brunetti, Giacomina, Acquafredda, Angelo, Delvecchio, Maurizio, Lonero, Antonella, Gaeta, Alberto, Suavo Bulzis, Paola, Corica, Domenico, Velletri, Maria Rosa, De Luca, Filippo, Cavallo, Luciano, and Wasniewska, Malgorzata

Subjects
GONADOTROPIN-inhibitory hormone, PRECOCIOUS puberty, THERAPEUTICS
Abstract

Background/Aims: Gonadotropin-releasing hormone analogues (GnRHa) represent the gold standard treatment for central precocious puberty (CPP). We aimed to assess the effects of GnRHa treatment on metabolic outcomes, bone status, and polycystic ovary syndrome (PCOS) prevalence in young girls with idiopathic CPP (ICPP). Methods: We enrolled 94 ICPP girls who were at least 2 years after menarche and had already attained adult height at the time of the study: 56 previously treated with depot triptorelin (3.4 ± 0.6 years) and 38 untreated. Auxological parameters, lipid profile, homeostatic model assessment of insulin resistance (HOMA-IR), bone state, and prevalence of PCOS were assessed. Results: The 2 groups were similar for body mass index (BMI) and waist circumference. HOMA-IR, dehydroepiandrosterone sulfate, and ▵ 4-androstenedione were higher in the treated than in the untreated subjects (p < 0.001). Significant differences were found for amplitude-dependent speed of sound (p < 0.03) and bone transmission time zscores (p < 0.01). The prevalence of PCOS was higher in the treated than in the untreated subjects (p < 0.04). Conclusion: GnRHa therapy is associated with hyperandrogenism and an increase in insulin resistance and PCOS prevalence, but not with increased BMI or lipid profile alterations. Long-term evaluations at the time of expected peak bone mass achievement are needed to understand the persistent or transient nature of subtle bone abnormalities. [ABSTRACT FROM AUTHOR]

Published
2017
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