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IntroductionAlzheimer’s disease (AD) is one of the most prevalent forms of dementia globally and remains an incurable condition that often leads to death. PANoptosis represents an emerging paradigm in programmed cell death, integrating three critical processes: pyroptosis, apoptosis, and necroptosis. Studies have shown that apoptosis, necroptosis, and pyroptosis play important roles in AD development. Therefore, targeting PANoptosis genes might lead to novel therapeutic targets and clinically relevant therapeutic approaches. This study aims to identify different molecular subtypes of AD and potential drugs for treating AD based on PANoptosis.MethodsDifferentially expressed PANoptosis genes associated with AD were identified via Gene Expression Omnibus (GEO) dataset GSE48350, GSE5281, and GSE122063. Least Absolute Shrinkage and Selection Operator (LASSO) regression was employed to construct a risk model linked to these PANoptosis genes. Consensus clustering analysis was conducted to define AD subtypes based on these genes. We further performed gene set variation analysis (GSVA), functional enrichment analysis, and immune cell infiltration analysis to investigate differences between the identified AD subtypes. Additionally, a protein-protein interaction (PPI) network was established to identify hub genes, and the DGIdb database was consulted to identify potential therapeutic compounds targeting these hub genes. Single-cell RNA sequencing analysis was utilized to assess differences in gene expression at the cellular level across subtypes.ResultsA total of 24 differentially expressed PANoptosis genes (APANRGs) were identified in AD, leading to the classification of two distinct AD subgroups. The results indicate that these subgroups exhibit varying disease progression states, with the early subtype primarily linked to dysfunctional synaptic signaling. Furthermore, we identified hub genes from the differentially expressed genes (DEGs) between the two clusters and predicted 38 candidate drugs and compounds for early AD treatment based on these hub genes. Single-cell RNA sequencing analysis revealed that key genes associated with the early subtype are predominantly expressed in neuronal cells, while the differential genes for the metabolic subtype are primarily found in endothelial cells and astrocytes.ConclusionIn summary, we identified two subtypes, including the AD early synaptic abnormality subtype as well as the immune-metabolic subtype. Additionally, ten hub genes, SLC17A7, SNAP25, GAD1, SLC17A6, SLC32A1, PVALB, SYP, GRIN2A, SLC12A5, and SYN2, were identified as marker genes for the early subtype. These findings may provide valuable insights for the early diagnosis of AD and contribute to the development of innovative therapeutic strategies.

Outlier detection is an important task in the field of data mining and a highly active area of research in machine learning. In industrial automation, datasets are often high-dimensional, meaning an effort to study all dimensions directly leads to data sparsity, thus causing outliers to be masked by noise effects in high-dimensional spaces. The “curse of dimensionality” phenomenon renders many conventional outlier detection methods ineffective. This paper proposes a new outlier detection algorithm called EOEH (Ensemble Outlier Detection Method Based on Information Entropy-Weighted Subspaces for High-Dimensional Data). First, random secondary subsampling is performed on the data, and detectors are run on various small-scale sub-samples to provide diverse detection results. Results are then aggregated to reduce the global variance and enhance the robustness of the algorithm. Subsequently, information entropy is utilized to construct a dimension-space weighting method that can discern the influential factors within different dimensional spaces. This method generates weighted subspaces and dimensions for data objects, reducing the impact of noise created by high-dimensional data and improving high-dimensional data detection performance. Finally, this study offers a design for a new high-precision local outlier factor (HPLOF) detector that amplifies the differentiation between normal and outlier data, thereby improving the detection performance of the algorithm. The feasibility of this algorithm is validated through experiments that used both simulated and UCI datasets. In comparison to popular outlier detection algorithms, our algorithm demonstrates a superior detection performance and runtime efficiency. Compared with the current popular, common algorithms, the EOEH algorithm improves the detection performance by 6% on average. In terms of running time for high-dimensional data, EOEH is 20% faster than the current popular algorithms.

The distribution of wireless network systems challenges the communication security of Internet of Things (IoT), and the IPv6 protocol is gradually becoming the main communication protocol under the IoT. The Neighbor Discovery Protocol (NDP), as the base protocol of IPv6, includes address resolution, DAD, route redirection and other functions. The NDP protocol faces many attacks, such as DDoS attacks, MITM attacks, etc. In this paper, we focus on the communication-addressing problem between nodes in the Internet of Things (IoT). We propose a Petri-Net-based NS flooding attack model for the flooding attack problem of address resolution protocols under the NDP protocol. Through a fine-grained analysis of the Petri Net model and attacking techniques, we propose another Petri-Net-based defense model under the SDN architecture, achieving security for communications. We further simulate the normal communication between nodes in the EVE-NG simulation environment. We implement a DDoS attack on the communication protocol by an attacker who obtains the attack data through the THC-IPv6 tool. In this paper, the SVM algorithm, random forest algorithm (RF) and Bayesian algorithm (NBC) are used to process the attack data. The NBC algorithm is proven to exhibit high accuracy in classifying and identifying data through experiments. Further, the abnormal data are discarded through the abnormal data processing rules issued by the controller in the SDN architecture, to ensure the security of communications between nodes.

Larvae of Chalcophora japonica chinensis Schaufuss, 1879 were collected from within dead trunks in Hubei Province, China, in February 2019. These specimens created an opportunity to provide the first description of the larval stage of this subspecies; The larva is described and illustrated based on morphological characters and DNA barcoding.

Obtaining the liquid storage state of oil wells in real time is very important for oilfield production. In this paper, under the premise of fully considering the transformation factors of full-pumping and nonfull-pumping states of oil wells, submergence depth models suitable for full- and nonfull-pumping wells are constructed. To reduce the application complexity of the models, parameter-reduction processing is performed to enhance the usability of the models. By analyzing the change trend of the submergence depth during the rising, maintaining, and falling of the oil well in the full-pumping state and nonfull-pumping state models, the judgment criteria for the transition of the oil well state are provided. On this basis, the application methods of nonlinear interpolation and least squares curve-fitting numerical solutions of submergence depth models are studied, and the unique existence of the solution of the corresponding one-variable nonlinear characteristic equation in the (0, 1) open interval is proven. Finally, the error estimation of the numerical solution is carried out, the calculation formula of the number of iterations for the numerical solution of the dichotomy is provided, and the error of the relevant numerical solution is verified.

Recent studies demonstrate that algorithmic music attracted global attention not only because of its amusement but also its considerable potential in the industry. Thus, the yield increased academic numbers spinning around on topics of algorithm music generation. The balance between mathematical logic and aesthetic value is important in music generation. To maintain this balance, we propose a research method based on a three-dimensional temporal convolutional attention neural network. This method uses a self-collected traditional Chinese pentatonic symbolic music dataset. It combines clustering algorithms and deep learning-related algorithms to construct a three-dimensional sequential convolutional generation model 3D-SCN, a three-dimensional temporal convolutional attention model BoYaTCN. We trained both of them to generate traditional Chinese pentatonic scale music that considers both overall temporal creativity and local musical semantics. Then, we conducted quantitative and qualitative evaluations of the generated music. The experiment demonstrates that BoYaTCN achieves the best results, with a prediction accuracy of 99.12%, followed by 3D-SCN with a prediction accuracy of 99.04%. We have proven that the proposed model can generate folk music with a beautiful melody, harmonious coherence, and distinctive traditional Chinese pentatonic features, and it also conforms to certain musical grammatical characteristics.

The detection and defense of malicious attacks are critical to the proper functioning of network security. Due to the diversity and rapid updates of the attack methods used by attackers, traditional defense mechanisms have been challenged. In this context, a more effective method to predict vulnerabilities in network systems is considered an urgent need to protect network security. In this paper, we propose a formal modeling and analysis approach based on Petri net vulnerability exploitation. We used the Common Vulnerabilities and Exposures (CVE)-2021-3711 vulnerability source code to build a model. A patch model was built to address the problems of this model. Finally, the time injected by the actual attacker and the time simulated by the software were calculated separately. The results showed that the simulation time was shorter than the actual attack time, and ultra-real-time simulation could be achieved. By modeling the network system with this method, the model can be found to arrive at an illegitimate state according to the structure of Petri nets themselves and thus discover unknown vulnerabilities. This method provides a reference method for exploring unknown vulnerabilities.

In this study, an analytical hierarchy process based model is proposed to perform the decision-making for virtual machine migration towards greeii cloud computing. The virtual machine migration evaluation index system is established based on the process of constructing hierarchies for evaluation of virtual machine migration, and selection of task usage. A comparative judgment of two hierarchies has been conducted. In the experimental study, five-point rating scale lias been adopted to map the raw data to the scaled rating score; this rating method is used to analyze the performance of each virtual machine and its task usage data. The results show a significant improvement in the decision-making process for the virtual machine migration. The deduced results are useful for the system administrators to migrate the exact virtual machine, and then switch on the power of physical machine that the migrated virtual machine resides on. Thus the proposed method contributes to the green cloud computing environment. [ABSTRACT FROM AUTHOR]