7 results on '"Leyva-Guerrero, Elisa"'
Search Results
2. Validation of a Method for Quantitation of Soybean Lectin in Commercial Varieties
- Author
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Breeze, Matthew L., Leyva-Guerrero, Elisa, Yeaman, Grant R., Dudin, Yelena, Akel, Ryan, Brune, Phil, Claussen, Fred, Dharmasri, Cecil, Golbach, Jenny, Guo, Rong, Maxwell, Carl, Privalle, Laura, Rogers, Hilary, Liu, Kai, Shan, Guomin, Yarnall, Michele, Thiede, Denise, and Gillikin, Nancy
- Published
- 2015
- Full Text
- View/download PDF
3. Extending Cassava Root Shelf Life via Reduction of Reactive Oxygen Species Production1[C][W][OA]
- Author
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Zidenga, Tawanda, Leyva-Guerrero, Elisa, Moon, Hangsik, Siritunga, Dimuth, and Sayre, Richard
- Subjects
Manihot ,Plant Stems ,Biochemical Processes and Macromolecular Structures ,Arabidopsis ,food and beverages ,Hydrogen Peroxide ,Plants, Genetically Modified ,Models, Biological ,Plant Roots ,Mitochondria ,Mitochondrial Proteins ,Plant Tubers ,Phenotype ,Nitriles ,Biomass ,Oxidoreductases ,Reactive Oxygen Species ,Plant Proteins ,Respiratory Burst - Abstract
One of the major constraints facing the large-scale production of cassava (Manihot esculenta) roots is the rapid postharvest physiological deterioration (PPD) that occurs within 72 h following harvest. One of the earliest recognized biochemical events during the initiation of PPD is a rapid burst of reactive oxygen species (ROS) accumulation. We have investigated the source of this oxidative burst to identify possible strategies to limit its extent and to extend cassava root shelf life. We provide evidence for a causal link between cyanogenesis and the onset of the oxidative burst that triggers PPD. By measuring ROS accumulation in transgenic low-cyanogen plants with and without cyanide complementation, we show that PPD is cyanide dependent, presumably resulting from a cyanide-dependent inhibition of respiration. To reduce cyanide-dependent ROS production in cassava root mitochondria, we generated transgenic plants expressing a codon-optimized Arabidopsis (Arabidopsis thaliana) mitochondrial alternative oxidase gene (AOX1A). Unlike cytochrome c oxidase, AOX is cyanide insensitive. Transgenic plants overexpressing AOX exhibited over a 10-fold reduction in ROS accumulation compared with wild-type plants. The reduction in ROS accumulation was associated with a delayed onset of PPD by 14 to 21 d after harvest of greenhouse-grown plants. The delay in PPD in transgenic plants was also observed under field conditions, but with a root biomass yield loss in the highest AOX-expressing lines. These data reveal a mechanism for PPD in cassava based on cyanide-induced oxidative stress as well as PPD control strategies involving inhibition of ROS production or its sequestration.
- Published
- 2012
4. Extending Cassava Root Shelf Life via Reduction of Reactive Oxygen Species Production1[C][W][OA].
- Author
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Zidenga, Tawanda, Leyva-Guerrero, Elisa, Moon, Hangsik, Siritunga, Dimuth, and Sayre, Richard
- Subjects
- *
CASSAVA research , *REACTIVE oxygen species , *POSTHARVEST diseases , *ARABIDOPSIS thaliana , *OXIDATIVE stress , *PHYSIOLOGY - Abstract
One of the major constraints facing the large-scale production of cassava (Manihot esculenta) roots is the rapid postharvest physiological deterioration (PPD) that occurs within 72 h following harvest. One of the earliest recognized biochemical events during the initiation of PPD is a rapid burst of reactive oxygen species (ROS) accumulation. We have investigated the source of this oxidative burst to identify possible strategies to limit its extent and to extend cassava root shelf life. We provide evidence for a causal link between cyanogenesis and the onset of the oxidative burst that triggers PPD. By measuring ROS accumulation in transgenic low-cyanogen plants with and without cyanide complementation, we show that PPD is cyanide dependent, presumably resulting from a cyanide-dependent inhibition of respiration. To reduce cyanide-dependent ROS production in cassava root mitochondria, we generated transgenic plants expressing a codon-optimized Arabidopsis (Arabidopsis thaliana) mitochondrial alternative oxidase gene (AOX1A). Unlike cytochrome c oxidase, AOX is cyanide insensitive. Transgenic plants overexpressing AOX exhibited over a 10-fold reduction in ROS accumulation compared with wild-type plants. The reduction in ROS accumulation was associated with a delayed onset of PPD by 14 to 21 d after harvest of greenhouse-grown plants. The delay in PPD in transgenic plants was also observed under field conditions, but with a root biomass yield loss in the highest AOX-expressing lines. These data reveal a mechanism for PPD in cassava based on cyanideinduced oxidative stress as well as PPD control strategies involving inhibition of ROS production or its sequestration. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
5. Extending Cassava Root Shelf Life via Reduction of Reactive Oxygen Species Production1[C][W][OA].
- Author
-
Zidenga, Tawanda, Leyva-Guerrero, Elisa, Moon, Hangsik, Siritunga, Dimuth, and Sayre, Richard
- Subjects
CASSAVA research ,REACTIVE oxygen species ,POSTHARVEST diseases ,ARABIDOPSIS thaliana ,OXIDATIVE stress ,PHYSIOLOGY - Abstract
One of the major constraints facing the large-scale production of cassava (Manihot esculenta) roots is the rapid postharvest physiological deterioration (PPD) that occurs within 72 h following harvest. One of the earliest recognized biochemical events during the initiation of PPD is a rapid burst of reactive oxygen species (ROS) accumulation. We have investigated the source of this oxidative burst to identify possible strategies to limit its extent and to extend cassava root shelf life. We provide evidence for a causal link between cyanogenesis and the onset of the oxidative burst that triggers PPD. By measuring ROS accumulation in transgenic low-cyanogen plants with and without cyanide complementation, we show that PPD is cyanide dependent, presumably resulting from a cyanide-dependent inhibition of respiration. To reduce cyanide-dependent ROS production in cassava root mitochondria, we generated transgenic plants expressing a codon-optimized Arabidopsis (Arabidopsis thaliana) mitochondrial alternative oxidase gene (AOX1A). Unlike cytochrome c oxidase, AOX is cyanide insensitive. Transgenic plants overexpressing AOX exhibited over a 10-fold reduction in ROS accumulation compared with wild-type plants. The reduction in ROS accumulation was associated with a delayed onset of PPD by 14 to 21 d after harvest of greenhouse-grown plants. The delay in PPD in transgenic plants was also observed under field conditions, but with a root biomass yield loss in the highest AOX-expressing lines. These data reveal a mechanism for PPD in cassava based on cyanideinduced oxidative stress as well as PPD control strategies involving inhibition of ROS production or its sequestration. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
6. Iron and protein biofortification of cassava: lessons learned
- Author
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Leyva-Guerrero, Elisa, Narayanan, Narayanan N, Ihemere, Uzoma, and Sayre, Richard T
- Subjects
- *
PROTEIN synthesis , *CASSAVA , *CALORIC content of foods , *BIOTECHNOLOGY , *GERMPLASM , *VITAMIN A , *PLANT roots - Abstract
Over two hundred and fifty million Africans rely on the starchy root crop cassava (Manihot esculenta) as their primary source of calories. Cassava roots, however, have the lowest protein:energy ratio of all the world''s major staple crops. Furthermore, a typical cassava-based diet provides less than 10–20% of the required amounts of iron, zinc, vitamin A and vitamin E. The BioCassava Plus program employed modern biotechnologies to improve the health of Africans through development and delivery of novel cassava germplasm with increased nutrient levels. Here we describe the development of molecular strategies and their outcomes to meet minimum daily allowances for protein and iron in cassava based diets. We demonstrate that cyanogens play a central role in cassava nitrogen metabolism and that strategies employed to increase root protein levels result in reduced cyanogen levels in roots. We also demonstrate that enhancing root iron uptake has an impact on the expression of genes that regulate iron homeostasis in multiple tissues. These observations demonstrate the complex metabolic interactions involved in enhancing targeted nutrient levels in plants and identify potential new strategies for further enhancing nutrient levels in cassava. [Copyright &y& Elsevier]
- Published
- 2012
- Full Text
- View/download PDF
7. Extending cassava root shelf life via reduction of reactive oxygen species production.
- Author
-
Zidenga T, Leyva-Guerrero E, Moon H, Siritunga D, and Sayre R
- Subjects
- Arabidopsis enzymology, Biomass, Hydrogen Peroxide metabolism, Manihot genetics, Manihot growth & development, Mitochondria metabolism, Mitochondrial Proteins metabolism, Models, Biological, Nitriles metabolism, Oxidoreductases metabolism, Phenotype, Plant Proteins metabolism, Plant Roots genetics, Plant Roots growth & development, Plant Roots metabolism, Plant Stems anatomy & histology, Plant Tubers growth & development, Plant Tubers metabolism, Plants, Genetically Modified, Respiratory Burst, Manihot physiology, Plant Roots physiology, Reactive Oxygen Species metabolism
- Abstract
One of the major constraints facing the large-scale production of cassava (Manihot esculenta) roots is the rapid postharvest physiological deterioration (PPD) that occurs within 72 h following harvest. One of the earliest recognized biochemical events during the initiation of PPD is a rapid burst of reactive oxygen species (ROS) accumulation. We have investigated the source of this oxidative burst to identify possible strategies to limit its extent and to extend cassava root shelf life. We provide evidence for a causal link between cyanogenesis and the onset of the oxidative burst that triggers PPD. By measuring ROS accumulation in transgenic low-cyanogen plants with and without cyanide complementation, we show that PPD is cyanide dependent, presumably resulting from a cyanide-dependent inhibition of respiration. To reduce cyanide-dependent ROS production in cassava root mitochondria, we generated transgenic plants expressing a codon-optimized Arabidopsis (Arabidopsis thaliana) mitochondrial alternative oxidase gene (AOX1A). Unlike cytochrome c oxidase, AOX is cyanide insensitive. Transgenic plants overexpressing AOX exhibited over a 10-fold reduction in ROS accumulation compared with wild-type plants. The reduction in ROS accumulation was associated with a delayed onset of PPD by 14 to 21 d after harvest of greenhouse-grown plants. The delay in PPD in transgenic plants was also observed under field conditions, but with a root biomass yield loss in the highest AOX-expressing lines. These data reveal a mechanism for PPD in cassava based on cyanide-induced oxidative stress as well as PPD control strategies involving inhibition of ROS production or its sequestration.
- Published
- 2012
- Full Text
- View/download PDF
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