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NoMix toilets separate urine and feces at the source and are a promising resource recovery technology. However, design issues hamper the transformation from unattractive to aspirational products. Little effort has been done to design toilets that account for physiological differences, leading to adverse effects on user-friendliness and urine separation efficiency. We used infrared recordings to assess gender-specific urination practices. Based on field data, we developed the Urinator, a simple device that allows simulating male and female urine streams. This supports engineers in developing more user- and gender-friendly and reuse-oriented sanitation technologies. [ABSTRACT FROM AUTHOR]

Not all the challenges of informal settlement upgrading programmes can be anticipated from the start. It calls for cumulative learning within the programme's timeline. This paper investigates the role of organizational learning in influencing programme outcomes. The analysis of the Kenya Informal Settlement Improvement Programme (KISIP) shows that a lack of organizational learning routines can lead to reduced programme success, and that programme learning can improve programme outcomes. Well-conceptualized processes that include participation, coordination, communication and the synthesis of information are essential, though insufficient alone. Additional barriers, including a sudden increase in the number and diversity of actors and projects, their deteriorating commitment, inequitable incentives and inadequate tools to support programme learning, can further exacerbate the absence of established programme learning routines. There is a need for explicit and transparent programme learning procedures across organizational levels in order to improve overall programme success. [ABSTRACT FROM AUTHOR]

The current initiative of the European Commission (EC) concerning plants produced using certain new genomic techniques, in particular, targeted mutagenesis and cisgenesis, underlines that a high level of protection for human and animal health and the environment needs to be maintained when using such applications. The current EU biosafety regulation framework ensures a high level of protection with a mandatory environmental risk assessment (ERA) of genetically modified (GM) products prior to the authorization of individual GMOs for environmental release or marketing. However, the guidance available from the European Food Safety Authority (EFSA) for conducting such an ERA is not specific enough regarding the techniques under discussion and needs to be further developed to support the policy goals towards ERA, i.e., a case-by-case assessment approach proportionate to the respective risks, currently put forward by the EC. This review identifies important elements for the case-by-case approach for the ERA that need to be taken into account in the framework for a risk-oriented regulatory approach. We also discuss that the comparison of genome-edited plants with plants developed using conventional breeding methods should be conducted at the level of a scientific case-by-case assessment of individual applications rather than at a general, technology-based level. Our considerations aim to support the development of further specific guidance for the ERA of genome-edited plants. [ABSTRACT FROM AUTHOR]

Citywide inclusive sanitation (CWIS) is becoming the dominant paradigm for achieving safe sanitation for all by 2030. Its technical benefits have been explored, but the bargaining over financial and organizational changes CWIS entails have not yet been adequately addressed. Our case study explains the stalled rollout of CWIS in Dhaka, Bangladesh. We analyse policy pathways over the past 30 years through a combined territorial political economy and power perspective to understand their effects on equality. We highlight how donors link the introduction of CWIS to the organization of sanitation through a market; how the utility uses CWIS as an opportunity to avoid costly responsibilities in non-sewered sanitation; and how service co-production through community-based solutions is neglected. CWIS has successfully overcome the dogmatic technological focus in the sanitation system, but for citywide sanitation to be scaled inclusively, the dogmatic focus in the organization and financing of the sanitation sector must also be overcome. [ABSTRACT FROM AUTHOR]

The water, sanitation, and solid waste sectors are closely related and have many interactions between their respective service chains in low- and middle-income countries. Currently, these interactions mostly lead to cross-contamination, and opportunities for co-benefits are seldom realized. This review presents the key advancements within each of these three development sectors in the past two decades. We identify numerous similarities such as decentralization, resource recovery, community involved planning, and digitalization. Despite the potential for synergies and the opportunities to maximize positive interactions, there have been few attempts to break the existing sectoral silos in order to integrate these three service chains. We argue that, with the right enabling environment, an integrated approachto holistically planning and implementing water supply, sanitation, and solid waste management can create positive interactions resulting in co-benefits among complementary development goals. [ABSTRACT FROM AUTHOR]

An intensely debated question is whether or how a mandatory environmental risk assessment (ERA) should be conducted for plants obtained through novel genomic techniques, including genome editing (GE). Some countries have already exempted certain types of GE applications from their regulations addressing genetically modified organisms (GMOs). In the European Union, the European Court of Justice confirmed in 2018 that plants developed by novel genomic techniques for directed mutagenesis are regulated as GMOs. Thus, they have to undergo an ERA prior to deliberate release or being placed on the market. Recently, the European Food Safety Authority (EFSA) published two opinions on the relevance of the current EU ERA framework for GM plants obtained through novel genomic techniques (NGTs). Regarding GE plants, the opinions confirmed that the existing ERA framework is suitable in general and that the current ERA requirements need to be applied in a case specific manner. Since EFSA did not provide further guidance, this review addresses a couple of issues relevant for the case-specific assessment of GE plants. We discuss the suitability of general denominators of risk/safety and address characteristics of GE plants which require particular assessment approaches. We suggest integrating the following two sets of considerations into the ERA: considerations related to the traits developed by GE and considerations addressing the assessment of method-related unintended effects, e.g., due to off-target modifications. In conclusion, we recommend that further specific guidance for the ERA and monitoring should be developed to facilitate a focused assessment approach for GE plants. [ABSTRACT FROM AUTHOR]

The water, sanitation and hygiene (WASH) sector is facing a shortfall of several million appropriately skilled professionals. Massive open online courses (MOOCs) can play a crucial role in addressing this. This paper presents the case study of the MOOC series "Sanitation, Water and Solid Waste for Development", which has reached over 120,000 learners within six years. It has attracted mainly well-educated, employed learners, under 34 years old, from Asia, Latin America and Africa. Underrepresentation of female learners remains a challenge. While MOOCs have proven excellent for delivering WASH education at scale, some alternative formats (e.g. blended learning, small private online courses) allow more collaborative, interactive learning environments. Three practical examples from Nigeria, Indonesia and Mozambique indicate the potential for synergies among MOOCs and further learning formats. With the global shift towards digital learning due to the COVID-19 pandemic, MOOCs have gained further traction. [ABSTRACT FROM AUTHOR]

Gene drive organisms (GDOs) have been suggested as approaches to combat some of the most pressing environmental and public health issues. No such organisms have so far been released into the environment, but it remains unclear whether the relevant regulatory provisions will be fit for purpose to cover their potential environmental, human and animal health risks if environmental releases of GDOs are envisaged. We evaluate the novel features of GDOs and outline the resulting challenges for the environmental risk assessment. These are related to the definition of the receiving environment, the use of the comparative approach, the definition of potential harm, the stepwise testing approach, the assessment of long-term and large-scale risks at population and ecosystem level and the post-release monitoring of adverse effects. Fundamental adaptations as well as the development of adequate risk assessment methodologies are needed in order to enable an operational risk assessment for globally spreading GDOs before these organisms are released into environments in the EU. [ABSTRACT FROM AUTHOR]

Health care facilities in low- and middle-income countries are high-risk settings, and face special challenges to achieving sustainable water, sanitation, and hygiene (WASH) services. Our applied interdisciplinary research conducted in India and Uganda analyzed six dimensions of WASH services in selected health care facilities, including menstrual hygiene management. To be effective, WASH monitoring strategies in health care facilities must include gender sensitive measures. We present a novel strategy, showing that applied gender sensitive multitool assessments are highly productive in assessments of WASH services and facilities from user and provider perspectives. We discuss its potential for applications at scale and as an area of future research. [ABSTRACT FROM AUTHOR]

BACKGROUND: Legumes have been genetically engineered to express α‐amylase inhibitor 1 (αAI‐1) from common bean in their seeds. Whereas the genetically modified (GM) seeds are immune to multiple bruchid pest species, the cosmopolitan bruchid Acanthoscelides obtectus is tolerant to αAI‐1 and their larvae develop normally inside the seeds. Hymenopteran bruchid parasitoids, the most important natural enemies of bruchids, might thus be exposed to αAI‐1 when attacking A. obtectus larvae developing inside GM seeds. Exposure might reduce parasitoid fitness, resulting in a decline in the natural control of A. obtectus, and thus promote the spread of this pest. We investigated the impact of the presence of αAI‐1 in legume seeds on parasitoid fitness in tritrophic experiments with αAI‐1 GM or non‐GM chickpea seeds, A. obtectus, and three parasitoid species. Additionally, we investigated the exposure of parasitoids to αAI‐1 using a fourth, highly sensitive parasitoid species. RESULTS: Parasitoid fitness was not affected when A. obtectus was used in GM chickpea seeds as hosts, and this lack of effects was probably attributable to the fact that exposure of the parasitoids to αAI‐1 was negligible. CONCLUSION: We conclude that the release of GM chickpeas containing αAI‐1 should not harm this important group of non‐target insects. © 2018 Society of Chemical Industry Bruchid parasitoids (in the picture: Heterospilus prosopidis) are not adversely affected by transgenic chickpeas producing the α‐amylase inhibitor αAI‐1 from the common bean for bruchid resistance. [ABSTRACT FROM AUTHOR]

The inadequate provision of sanitation in informal urban settlements, also known as slums, continues to be an important issue. New technologies and services are being designed to solve this problem. However, the history of failed sanitation programmes and projects highlights a lack of understanding of how slum inhabitants decide on investing in such products and services. In this paper, we gather perspectives from potential clients and investigate how slum inhabitants (1) perceive the current situation and whether they desire improvements of sanitation, (2) how they evaluate a new toilet that is still in development, and how (3) social processes and (4) constraints affect decisions. Data were collected through interviewing 1538 people within a general household survey. People using shared and public latrines desire an improvement of their sanitation facilities. The lack of water for washing is perceived by residents as a the biggest problem when accessing current latrines. The new toilet was mostly evaluated positively: people like it, expect large health benefits from it and it complies with cultural norms. However, people also expect some problems with the functioning of the toilet and expect opposition to pay for the service, due to the high costs and a lack of space to set up new toilets. [ABSTRACT FROM AUTHOR]

The improvement of sanitation conditions in slums(1) is difficult in part because of economic and institutional environments that often prevent private or public investment in infrastructure. This analysis of sanitation conditions in informal settlements in Nairobi and Kampala compares user practices. It also identifies the main actors involved in the provision of overlapping sanitation systems, involving a multitude of small-scale providers, along with the challenges these present. The paper goes on to describe a new market-based approach in Nairobi, developed by a social enterprise, Sanergy, which is responsible for the construction of facilities and the collection and treatment of wastes. The system improves user satisfaction, community wellbeing and environmental quality, pointing to a clear opportunity for such market-based interventions where a customer base already pays for sub-standard services. There remain challenges, however, around the ability to scale up and finance these providers, especially in complex operating environments that increase transactional costs for companies. [ABSTRACT FROM AUTHOR]

Dry grain legume seeds possessing αAI-1, an α-amylase inhibitor from common bean (Phaseolus vulgaris), under the control of a cotyledon-specific promoter have been shown to be highly resistant to several important bruchid pest species. One transgenic chickpea and four cowpea lines expressing αAI-1, their respective controls, as well as nine conventional chickpea cultivars were assessed for their resistance to the bruchids Acanthoscelides obtectus (Say), Callosobruchus chinensis L. and Callosobruchus maculatus F. All transgenic lines were highly resistant to both Callosobruchus species. A. obtectus, known to be tolerant to αAI-1, was able to develop in all transgenic lines. While the cotyledons of all non-transgenic cultivars were highly susceptible to all bruchids, C. chinensis and C. maculatus larvae suffered from significantly increased mortality rates inside transgenic seeds. The main factor responsible for the partial resistance in the non-transgenic cultivars was deduced to reside in the seed coat. The αAI-1 present in seeds of transgenic chickpea and cowpea lines significantly increases their resistance to two important bruchid pest species (C. chinensis and C. maculatus) essentially to immunity. To control αAI-1 tolerant bruchid species such as A. obtectus and to avoid the development of resistance to αAI-1, varieties carrying this transgene should be protected with additional control measures. [ABSTRACT FROM AUTHOR]

Genetically modified (GM) cowpea seeds expressing αAI-1, an α-amylase inhibitor from the common bean, have been shown to be immune against several bruchid species. Effective control of such pests by growing GM cowpea could promote the spread of bruchid species that are αAI-1 tolerant. Consequently, the sustainability of bruchid pest control could be increased by combining GM seeds and hymenopteran parasitoids. However, there are concerns that αAI-1 could interfere with the biological control provided by parasitoids. Here, we assessed the impact of GM cowpea seeds expressing αAI-1 on the αAI-1-tolerant bruchid Zabrotes subfasciatus and its parasitoid Dinarmus basalis. αAI-1 in cowpea seeds did not increase resistance to Z. subfasciatus or affect the mortality rate of Z. subfasciatus larvae. Parasitism of Z. subfasciatus by D. basalis and fitness of D. basalis offspring were not affected by the presence of αAI-1. Thus, αAI-1-expressing cowpeas and parasitoids should be compatible for the control of bruchid pests. [ABSTRACT FROM AUTHOR]

Genetically modified (GM) legumes expressing the a-amylase inhibitor 1 (aAI-1) from Phaseolus vulgaris L. or cysteine protease inhibitors are resistant to several bruchid pests (Coleoptera: Chrysomelidae). In addition, the combination of plant resistance factors together with hymenopteran parasitoids can substantially increase the bruchid control provided by the resistance alone. If the strategy of combining a bruchid-resistant GM legume and biological control is to be effective, the insecticidal trait must not adversely affect bruchid antagonists. The environmental risk assessment of such GM legumes includes the characterization of the targeted enzymes in the beneficial species and the assessment of the in vitro susceptibility to the resistance factor. The digestive physiology of bruchid parasitoids remain relatively unknown, and their susceptibility to aAI-1 has never been investigated. We have detected a-amylase and serine protease activities in all five bruchid parasitoid species tested. Thus, the deployment of GM legumes expressing cysteine protease inhibitors to control bruchids should be compatible with the use of parasitoids. In vitro inhibition studies showed that sensitivity of a-amylase activity to aAI-1 in the parasitoids was comparable to that in the target species. Direct feeding assays revealed that harmful effects of a-amylase inhibitors on bruchid parasitoids cannot be discounted and need further evaluation. [ABSTRACT FROM AUTHOR]

Urban sanitation presents one of the most significant service delivery challenges related to poverty alleviation and sustainable development in the developing world. The past decade has witnessed innovations in service delivery approaches for unserved rural and urban settlements with a clear policy shift to community-based approaches that attempt to overcome the supply-led, over-engineered sanitation solutions of the past decades. This article presents two examples of new developments: the urban-focussed household-centred environmental sanitation (HCES) and the rural-focussed community-led total sanitation (CLTS) approaches. The internationally renowned CLTS approach has achieved considerable success since its introduction, by harnessing community and small private sector capacity to solve sanitation problems locally. Experience with validation of the HCES approach in a variety of urban sites in Africa, Asia and Latin America is presented in the second part of the article highlighting some of the lessons learned. The article closes by arguing that a combination of HCES and CLTS, two field-tested methodologies, has the potential to improve the sustainability of sanitation service interventions. [ABSTRACT FROM AUTHOR]

The front cover image is based on the Research Article The bean α‐amylase inhibitor αAI‐1 in genetically modified chickpea seeds does not harm parasitoid wasps by Christoph Lüthi et al., DOI: 10.1002/ps.4919. Source Image: © Dr. Christopher B. Jackson, IKELOS. The front cover image is based on the Research Article The bean α‐amylase inhibitor αAI‐1 in genetically modified chickpea seeds does not harm parasitoid wasps by Christoph Lüthi et al., DOI: 10.1002/ps.4919. Source Image: © Dr. Christopher B. Jackson, IKELOS. [ABSTRACT FROM AUTHOR]

The banana tree circle (BTC) is a low-cost system for local greywater management, using a natural treatment and disposal process, providing additional resource recovery benefits. However, there are no standard design criteria for BTC that would allow for quality control of its efficiency and sustainability, and little is currently known about the full-scale performance of BTC. Based on the scoping literature review of 31 documents in the scientific database and eight documents from grey literature, a standard design model was proposed for the BTC technology based on the concept of water balance, greywater flows, rain, infiltration, and evapotranspiration. The first two steps of the BTC design were determining the areas required for infiltration and evapotranspiration. A cylindrical form trench, the soil percolation rate, and the hydraulic loading rate were considered for the infiltration area. The banana trees' evapotranspiration rate was taken into consideration for the evapotranspiration area. The proposed model was applied in a case study where we used a trench with 0.8 m depth and 1.5 m diameter. This study proposes a standard design criterion for the BTC based on environmental factors, and the scoping of the literature provides the basis for future studies to evaluate its environmental sustainability., Competing Interests: The authors declare there is no conflict., (© 2024 The Authors This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC BY 4.0), which permits copying, adaptation and redistribution, provided the original work is properly cited (http://creativecommons.org/licenses/by/4.0/).)

A majority of the world's population use onsite sanitation systems, which store or treat excreta close to where it is generated. Sludge from these systems needs to be managed through a series of stages, known as the sanitation value chain. There is a huge diversity of service providers, not only within each part of the chain, but also along the chain bridging the different components. These service providers are linked not only by the flow of materials, but also by the transfer of money. Therefore for this system to be considered financially sustainable all services from the toilet to reuse or disposal need to be considered. A tool has been developed (eSOSView ™ ) to simulate, evaluate, and optimise the financial flows along and within the sanitation value chain. In this paper eSOSView™ was tested, validated (using existing data), and piloted (including data collection). This paper demonstrates how eSOSView ™ can be used to evaluate different financial flow models, to assess financial sustainability in different parts of the sanitation value chain and optimise the financial sustainability along the sanitation value chain.