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Scholars have argued that 21st century educators have the responsibility to incorporate global citizenship activities into academic curricula to meet the demands of an increasingly diverse world. Approaches to global citizenship education that have rendered positive results include service-learning, critical thinking activities, second language acquisition, and international exchanges. The current study explores a Collaborative Online International Learning (COIL) project carried out between university students enrolled in universities located in the Arizona-Sonora Megaregión. The study found that before students participated in COIL they demonstrated a limited understanding of their neighboring country. At the conclusion of the COIL project students demonstrated global citizenship skills such as the ability to analyze international relationships, critically consume media, and make identify points of global interconnectedness.

International non-profit organizations (INGOs) were traditionally established to facilitate dialogue and bridge cultures. In order for INGOs to successfully carry out their objectives, global activists must work together in a culturally sensitive and responsible manner. The current article examines a volunteer training model that was designed to promote meaningful interactions between binational volunteers. The training, based on a Spanish-immersion and homestay experience, was piloted among volunteers at an INGO on the Mexico-US border. Results suggest that the immersion training model allowed volunteers to reassess and ultimately strengthen their relationships with their international counterparts while developing intercultural citizenship skills.

In order to prepare students to successfully engage with native speakers and members of heritage language communities both at home and abroad, educators must develop course curricula that emphasize cultural practices, products, perspectives, and comparisons as well as provide students with opportunities to directly apply the skills that they have acquired in the classroom across a range of other settings. This article describes a Culture for the Professions course whose curriculum was based on the four steps of the cultural intelligence model (Earley & Ang, 2003): drive, knowledge, strategy, and action. The study focuses in particular on how students implemented the last two components of the model (strategy and action) by collaborating with local organizations to design and present culture workshops that were specifically tailored to each organization's needs and mission. In addition to benefiting the organizations themselves, data obtained from evaluation rubrics and postworkshop reflections suggest that community culture workshops allow students to become more aware of how culture influences the work of an organization and to develop strategies to bridge cultural differences in the workplace.

Objetivou-se avaliar diferentes fontes energéticas em suplementos múltiplos no período da seca, sobre os ganhos de peso total (GPT), ganho médio diário (GMD), peso vivo final (PVF), consumo de suplemento e custos de produção. Avaliaram-se suplementos à base de farelo de soja + casca de soja (SCS); farelo de soja + grão de milho moído (SM); farelo de soja + grão de sorgo moído (SS); farelo de soja + grão de milheto moído (SMI). Foram utilizados 20 novilhos, com peso médio inicial de 160 kg. Os suplementos foram fornecidos diariamente na quantidade de 1 kg/animal/dia às 10h da manhã. No primeiro dia de cada período experimental, foram feitas coletas de forragem para estimar a disponibilidade de matéria seca/ha e determinar a composição químico-bromatológica da forragem ingerida pelos animais. As disponibilidades médias de matéria seca total (MST), matéria seca potencialmente digestível (MSpD), matéria seca de folhas verdes (MSFV), matéria seca de folhas secas (MSFS), matéria seca de colmo verde (MSCV) e matéria seca de colmo seco (MSCS) de pastagens Brachiaria brizantnha cv. Marandu foram de 5.718; 3.692; 588; 1.966; 1.788 e 1.375 kg/ha. Os consumos de suplemento foram de 0,951; 0,930; 0,976 e 0,886 kg/animal/dia, respectivamente, para os tratamentos SCS, SM, SS e SMI. Não se verificou diferença (P>0,05) entre os tratamentos para PVF, GPT e GMD. Os GMD, para os tratamentos SS, SMI, SM e SCS, foram, respectivamente, de 0,751; 0,713; 0,752 e 0,786 kg/animal/dia. O tratamento SCS proporcionou ganhos de peso com menor custo.

Scholars have argued that 21st century educators have the responsibility to incorporate global citizenship activities into academic curricula to meet the demands of an increasingly diverse world. Approaches to global citizenship education that have rendered positive results include service-learning, critical thinking activities, second language acquisition, and international exchanges. The current study explores a Collaborative Online International Learning (COIL) project carried out between university students enrolled in universities located in the Arizona-Sonora Megaregión. The study found that before students participated in COIL they demonstrated a limited understanding of their neighboring country. At the conclusion of the COIL project students demonstrated global citizenship skills such as the ability to analyze international relationships, critically consume media, and make identify points of global interconnectedness. [ABSTRACT FROM AUTHOR]

International non-profit organizations (INGOs) were traditionally established to facilitate dialogue and bridge cultures. In order for INGOs to successfully carry out their objectives, global activists must work together in a culturally sensitive and responsible manner. The current article examines a volunteer training model that was designed to promote meaningful interactions between binational volunteers. The training, based on a Spanish-immersion and homestay experience, was piloted among volunteers at an INGO on the Mexico-US border. Results suggest that the immersion training model allowed volunteers to reassess and ultimately strengthen their relationships with their international counterparts while developing intercultural citizenship skills. [ABSTRACT FROM AUTHOR]

This brief description of learning disabilities in Colombia, South America, notes the lack of government recognition of learning disability as distinct from mental retardation, the lack of appropriate educational services, the recent formation of the Colombian Foundation for Learning Disabilities, and the establishment of a special school. (DB)

In order to prepare students to successfully engage with native speakers and members of heritage language communities both at home and abroad, educators must develop course curricula that emphasize cultural practices, products, perspectives, and comparisons as well as provide students with opportunities to directly apply the skills that they have acquired in the classroom across a range of other settings. This article describes a Culture for the Professions course whose curriculum was based on the four steps of the cultural intelligence model (Earley & Ang, 2003): drive, knowledge, strategy, and action. The study focuses in particular on how students implemented the last two components of the model (strategy and action) by collaborating with local organizations to design and present culture workshops that were specifically tailored to each organization's needs and mission. In addition to benefiting the organizations themselves, data obtained from evaluation rubrics and postworkshop reflections suggest that community culture workshops allow students to become more aware of how culture influences the work of an organization and to develop strategies to bridge cultural differences in the workplace. [ABSTRACT FROM AUTHOR]

OBJECTIVE: Spiritual and religious factors may influence mental health in midlife women. The purpose of this study was to explore whether strength of religious beliefs or attendance at religious services helps to mitigate the stresses of life in mid-life women. METHODS: Data are from a sub-sample of 265 women, ages 40-70, who were participants in the REACH study, a longitudinal study investigating health parameters in a representative sample of households from rural communities in eastern North Carolina. Using t-tests and linear regression analyses, we analyzed the relationship between frequency of attendance at religious services and strength of religious beliefs in 1997 and subsequent mental health in 2003 as measured by the mental health component score (MCS) of the SF-12. RESULTS: The mean MCS in 2003 was significantly higher (better mental health) in women who reported attending religious services > or =1/week compared to those who reported attending <1/week (53.9 vs. 51.7; p < 0.05). In the linear regression model controlling for self-reported health status, baseline attendance at organized religious services remained a significant predictor of the MCS at six-year follow-up (standardized beta = -0.123, p < 0.05). CONCLUSIONS: Attendance at religious services is positively related to subsequent mental health in middle-aged women. The findings support the notion that religious commitment may help mitigate the stress of the midlife period. More research is needed to translate these findings into clinical interventions that can decrease the burden of anxiety and depression on midlife women. [ABSTRACT FROM AUTHOR]

When no organic cause for dyspepsia is found, the condition generally is considered to be functional, or idiopathic. Nonulcer dyspepsia can cause a variety of symptoms, including abdominal pain, bloating, nausea, and vomiting. Many patients with nonulcer dyspepsia have multiple somatic complaints, as well as symptoms of anxiety and depression. Extensive diagnostic testing is not recommended, except in patients with serious risk factors such as dysphagia, protracted vomiting, anorexia, melena, anemia, or a palpable mass. In these patients, endoscopy should be considered to exclude gastroesophageal reflux disease, peptic or duodenal ulcer, and gastric cancer. In patients without risk factors, consideration should be given to empiric therapy with a prokinetic agent (e.g., metoclopramide), an acid suppressant (histamine-H2 receptor antagonist), or an antimicrobial agent with activity against Helicobacter pylori. Treatment of patients with H. pylori infection and nonulcer dyspepsia (rather than peptic ulcer) is controversial and should be undertaken only when the pathogen has been identified. Psychotropic agents should be used in patients with comorbid anxiety or depression. Treatment of nonulcer dyspepsia can be challenging because of the need to balance medical management strategies with treatments for psychologic or functional disease. [ABSTRACT FROM AUTHOR]

BACKGROUND: The Association of American Medical Colleges has recommended addressing spirituality in the medical curriculum. DESCRIPTION: To evaluate the impact of a spiritual history-taking curriculum on the skills, knowledge, and attitudes of 1st year medical students. The study implemented a spiritual history-taking curriculum in the 1st year of medical school that included reading assignments, practice history taking, and standardized patient (SP) scenarios with spiritual content. It assessed students' performance in three ways: (a) using a videotaped SP interview, (b) a survey of students' attitudes regarding incorporating patients' religious and cultural views into medical decision making, and (c) a written test question on their first examination. EVALUATION: Students (146) took part in the medical school's spirituality curriculum, which included participation in videotaped interviews; 98% completed the initial survey, and 75% completed the follow-up survey. On the final videotaped SP interview, 65% of students were able to recognize the patient's spiritual concern according to trained faculty observers. On the attitude survey, there was an increased desire to accommodate patients' beliefs, although the magnitude of the increase was generally quite small. Ninety-four percent of students answered the test question correctly. CONCLUSION: Spiritual history taking can be integrated effectively into the existing history-taking curriculum in 1st year medical training. [ABSTRACT FROM AUTHOR]

OBJECTIVE: The purpose of this study was to determine whether a relationship exists between religious attendance and continuity of care. METHODS: We investigated this relationship in a large national database--the National Health and Nutrition Examination Survey III--which contains information on religious attendance, continuity of care, and potential confounders that might mediate the relationship such as health status and socioeconomic status. RESULTS: A total of 18,162 participants 17 years of age or older were included in the analysis. People who attended religious services were more likely to have continuity with a provider than people who did not attend such services (65% vs. 35%, p < .001). This association persisted after controlling for demographic, socioeconomic, and health status variables using regression analysis. CONCLUSION: This study documents a significant association between attendance at religious services and continuity with a usual provider. Continuity with a regular medical provider may influence the association of religious attendance and health outcomes. [ABSTRACT FROM AUTHOR]

PURPOSE: To examine the relationship between elevated inflammatory markers (CRP, fibrinogen, and white blood cell levels) and various forms of exercise for the adult U.S. population while controlling for factors that might influence the relationship. METHODS: An analysis of the adults age 17 and over who participated in the National Health and Nutrition Examination Survey (NHANES) III was conducted. The main goal of the analysis was to determine whether exercise type was associated with systemic markers of inflammation. Bivariate statistics using chi-square to evaluate different types of exercise according to the presence of elevated and nonelevated inflammatory markers was initially performed. In addition, multivariate models were constructed using each type of exercise activity as the predictor variable and each inflammatory marker as the dependent variable. RESULTS: A total of 4072 people were included in the analysis. In bivariate analyses, compared with nonexercisers in a specific exercise type, a significant lower likelihood of elevated inflammatory markers was found among regular participants in jogging, swimming, cycling, aerobic dancing, calisthenics, and weight lifting but not for gardening. After controlling for possible confounding factors including age, race, sex, body mass index, smoking, and health status in logistic regression analysis, only regular participants in jogging and aerobic dancing remained significantly less likely to have elevated cardiovascular markers. CONCLUSIONS: The results of this study indicate that some forms of physical activity are associated with a lower likelihood of elevation of inflammatory markers, although we cannot exclude the possibility that differences may be due to exercise intensity or duration. Future research should be directed toward further exploration of the effects of different types of exercise activity on inflammatory markers and the role of exercise in the prevention of cardiovascular disease. [ABSTRACT FROM AUTHOR]

Family physicians should be familiar with the acute management of atrial fibrillation and the initiation of chronic therapy for this common arrhythmia. Initial management should include hemodynamic stabilization, rate control, restoration of sinus rhythm, and initiation of antithrombotic therapy. Part II of this two-part article focuses on the prevention of thromboembolic complications using anticoagulation. Heparin is routinely administered before medical or electrical cardioversion. Warfarin is used in patients with persistent atrial fibrillation who are at higher risk for thromboembolic complications because of advanced age, history of coronary artery disease or stroke, or presence of left-sided heart failure. Aspirin is preferred in patients at low risk for thromboembolic complications and patients with a high risk for falls, a history of noncompliance, active bleeding, or poorly controlled hypertension. The recommendations provided in this article are consistent with guidelines published by the American Heart Association and the Agency for Healthcare Research and Quality. [ABSTRACT FROM AUTHOR]

Atrial fibrillation is the arrhythmia most commonly encountered in family practice. Serious complications can include congestive heart failure, myocardial infarction, and thromboembolism. Initial treatment is directed at controlling the ventricular rate, most often with a calcium channel blocker, a beta blocker, or digoxin. Medical or electrical cardioversion to restore sinus rhythm is the next step in patients who remain in atrial fibrillation. Heparin should be administered to hospitalized patients undergoing medical or electrical cardioversion. Anticoagulation with warfarin should be used for three weeks before elective cardioversion and continued for four weeks after cardioversion. The recommendations provided in this two-part article are consistent with guidelines published by the American Heart Association and the Agency for Healthcare Research and Quality. [ABSTRACT FROM AUTHOR]

Acute exacerbations of chronic obstructive pulmonary disease (COPD) are treated with oxygen (in hypoxemic patients), inhaled beta2 agonists, inhaled anticholinergics, antibiotics and systemic corticosteroids. Methylxanthine therapy may be considered in patients who do not respond to other bronchodilators. Antibiotic therapy is directed at the most common pathogens, including Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis. Mild to moderate exacerbations of COPD are usually treated with older broad-spectrum antibiotics such as doxycycline, trimethoprim-sulfamethoxazole and amoxicillin-clavulanate potassium. Treatment with augmented penicillins, fluoroquinolones, third-generation cephalosporins or aminoglycosides may be considered in patients with more severe exacerbations. The management of chronic stable COPD always includes smoking cessation and oxygen therapy. Inhaled beta2 agonists, inhaled anticholinergics and systemic corticosteroids provide short-term benefits in patients with chronic stable disease. Inhaled corticosteroids decrease airway reactivity and reduce the use of health care services for management of respiratory symptoms. Preventing acute exacerbations helps to reduce long-term complications. Long-term oxygen therapy, regular monitoring of pulmonary function and referral for pulmonary rehabilitation are often indicated. Influenza and pneumococcal vaccines should be given. Patients who do not respond to standard therapies may benefit from surgery. [ABSTRACT FROM AUTHOR]

Controlling diabetes requires patients to participate in a number of self-management tasks, many of which impose demands and restrictions. For better success, learn how to implement feasible programs. [ABSTRACT FROM AUTHOR]

Community-acquired pneumonia is a common infection encountered in clinical practice and is the leading cause of death due to infectious disease in the United States. To choose initial antimicrobial therapy appropriately, physicians must keep informed of recent developments in the epidemiology and clinical manifestations of atypical pathogens, antibiotic resistance and new antibiotics. Differentiating between 'typical' and 'atypical' pneumonia based on the initial clinical presentation is difficult. Primary treatment for community-acquired pneumonia in young adults without comorbidities is erythromycin. Older adults or those with comorbidities should be treated with erythromycin plus trimethoprim-sulfamethoxazole, a second- or third-generation cephalosporin, or a new macrolide. Empiric therapy for moderately or severely ill hospitalized patients usually begins with erythromycin plus a second- or third-generation cephalosporin. Physicians should use initial antibiotic therapy for coverage of both typical and atypical pathogens. [ABSTRACT FROM AUTHOR]

Background: Sex differences in mitochondrial function have been reported in multiple tissue and cell types. Additionally, sex-variable responses to stressors including environmental pollutants and drugs that cause mitochondrial toxicity have been observed. The mechanisms that establish these differences are thought to include hormonal modulation, epigenetic regulation, double dosing of X-linked genes, and the maternal inheritance of mtDNA. Understanding the drivers of sex differences in mitochondrial function and being able to model them in vitro is important for identifying toxic compounds with sex-variable effects. Additionally, understanding how sex differences in mitochondrial function compare across species may permit insight into the drivers of these differences, which is important for basic biology research. This study explored whether Caenorhabditis elegans, a model organism commonly used to study stress biology and toxicology, exhibits sex differences in mitochondrial function and toxicant susceptibility. To assess sex differences in mitochondrial function, we utilized four male enriched populations (N2 wild-type male enriched, fog-2(q71), him-5(e1490), and him-8(e1498)). We performed whole worm respirometry and determined whole worm ATP levels and mtDNA copy number. To probe whether sex differences manifest only after stress and inform the growing use of C. elegans as a mitochondrial health and toxicologic model, we also assessed susceptibility to a classic mitochondrial toxicant, rotenone., Results: We detected few to no large differences in mitochondrial function between C. elegans sexes. Though we saw no sex differences in vulnerability to rotenone, we did observe sex differences in the uptake of this lipophilic compound, which may be of interest to those utilizing C. elegans as a model organism for toxicologic studies. Additionally, we observed altered non-mitochondrial respiration in two him strains, which may be of interest to other researchers utilizing these strains., Conclusions: Basal mitochondrial parameters in male and hermaphrodite C. elegans are similar, at least at the whole-organism level, as is toxicity associated with a mitochondrial Complex I inhibitor, rotenone. Our data highlights the limitation of using C. elegans as a model to study sex-variable mitochondrial function and toxicological responses., (© 2024. The Author(s).)

The field of environmental epigenetics is uniquely suited to investigate biologic mechanisms that have the potential to link stressors to health disparities. However, it is common practice in basic epigenetic research to treat race as a covariable in large data analyses in a way that can perpetuate harmful biases without providing any biologic insight. In this article, we i) propose that epigenetic researchers open a dialogue about how and why race is employed in study designs and think critically about how this might perpetuate harmful biases; ii) call for interdisciplinary conversation and collaboration between epigeneticists and social scientists to promote the collection of more detailed social metrics, particularly institutional and structural metrics such as levels of discrimination that could improve our understanding of individual health outcomes; iii) encourage the development of standards and practices that promote full transparency about data collection methods, particularly with regard to race; and iv) encourage the field of epigenetics to continue to investigate how social structures contribute to biological health disparities, with a particular focus on the influence that structural racism may have in driving these health disparities., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 King, Lalwani, Mercado, Dolan, Frierson, Meyer and Murphy.)

Apoptosis and clearance of apoptotic cells via efferocytosis are evolutionarily conserved processes that drive tissue repair. However, the mechanisms by which recognition and clearance of apoptotic cells regulate repair are not fully understood. Here, we use single-cell RNA sequencing to provide a map of the cellular dynamics during early inflammation in mouse skin wounds. We find that apoptotic pathways and efferocytosis receptors are elevated in fibroblasts and immune cells, including resident Lyve1 + macrophages, during inflammation. Interestingly, human diabetic foot wounds upregulate mRNAs for efferocytosis pathway genes and display altered efferocytosis signaling via the receptor Axl and its ligand Gas6 . During early inflammation in mouse wounds, we detect upregulation of Axl in dendritic cells and fibroblasts via TLR3-independent mechanisms. Inhibition studies in vivo in mice reveal that Axl signaling is required for wound repair but is dispensable for efferocytosis. By contrast, inhibition of another efferocytosis receptor, Timd4, in mouse wounds decreases efferocytosis and abrogates wound repair. These data highlight the distinct mechanisms by which apoptotic cell detection coordinates tissue repair and provides potential therapeutic targets for chronic wounds in diabetic patients., Competing Interests: OJ, KB, WK, MF, QP, TS, KC, DK, HH, MG, SV, RD, KM No competing interests declared, VH Reviewing editor, eLife, (© 2023, Justynski et al.)

Lead (Pb 2+ ) is an important developmental toxicant. The mitochondrial calcium uniporter (MCU) imports calcium ions using the mitochondrial membrane potential (MMP), and also appears to mediate the influx of Pb 2+ into the mitochondria. Since our environment contains mixtures of toxic agents, it is important to consider multi-chemical exposures. To begin to develop generalizable, predictive models of interactive toxicity, we developed mechanism-based hypotheses about interactive effects of Pb 2+ with other chemicals. To test these hypotheses, we exposed HepG2 (human liver) cells to Pb 2+ alone and in mixtures with other mitochondria-damaging chemicals: carbonyl cyanide- p -trifluoromethoxyphenylhydrazone (FCCP), a mitochondrial uncoupler that reduces MMP, and Ruthenium Red (RuRed), a dye that inhibits the MCU. After 24 hours, Pb 2+ alone, the mixture of Pb 2+ and RuRed, and the mixture of Pb 2+ and FCCP caused no decrease in cell viability. However, the combination of all three exposures led to a significant decrease in cell viability at higher Pb 2+ concentrations. After 48 hours, the co-exposure to elevated Pb 2+ concentrations and FCCP caused a significant decrease in cell viability, and the mixture of all three showed a clear dose-response curve with significant decreases in cell viability across a range of Pb 2+ concentrations. We performed ICP-MS analyses on isolated mitochondrial and cytosolic fractions and found no differences in Pb 2+ uptake across exposure groups, ruling out altered cellular uptake as the mechanism for interactive toxicity. We assessed MMP following exposure and observed a decrease in membrane potential that corresponds to loss of cell viability but is likely not sufficient to be the causative mechanistic driver of cell death. This research provides a mechanistically-based framework for understanding Pb 2+ toxicity in mixtures with mitochondrial toxicants.

Collagen mutations are commonly used in the creation of Caenorhabditis elegans transgenic strains, but their secondary effects are not fully characterized . We compared the mitochondrial function of N2, dpy-10, rol-6, and PE255 C. elegans . N2 worms exhibited ~2-fold greater volume, mitochondrial DNA copy number, and nuclear DNA copy number than collagen mutants (p<0.05). Whole-worm respirometry and ATP levels were higher in N2 worms, but differences in respirometry largely disappeared after normalization to mitochondrial DNA copy number. This data suggests that rol-6 and dpy-10 mutants are developmentally delayed but have comparable mitochondrial function to N2 worms once the data is normalized to developmental stage., Competing Interests: The authors declare that there are no conflicts of interest present., (Copyright: © 2023 by the authors.)

Sex-linked differences in mitochondrial ATP production, enzyme activities, and reactive oxygen species generation have been reported in multiple tissue and cell types. While the effects of reproductive hormones underlie many of these differences, regulation of sexually dimorphic mitochondrial function has not been fully characterized. We hypothesized that sex-specific DNA methylation contributes to sex-specific expression of nuclear genes that influence mitochondrial function. Herein, we analysed DNA methylation data specifically focused on nuclear-encoded mitochondrial genes in 191 males and 190 females. We found 596 differentially methylated sites (DMSs) (FDR p < 0.05), corresponding to 324 genes, with at least a 1% difference in methylation between sexes. To investigate the potential functional significance, we utilized gene expression microarray data. Of the 324 genes containing DMSs, 17 showed differences in gene expression by sex. Particularly striking was that ATP5G2 , encoding subunit C of ATP synthase, contains seven DMSs and exhibits a sex difference in expression (p = 0.04). Finally, we also found that alterations in DNA methylation associated with in utero tobacco smoke exposure were sex-specific in these nuclear-encoded mitochondrial genes. Interestingly, the level of sex differences in DNA methylation at nuclear-encoded mitochondrial genes and the level of methylation changes associated with smoke exposure were less prominent than that of other genes. This suggests more conservative regulation of DNA methylation at these nuclear-encoded mitochondrial genes as compared to others. Overall, our findings suggest that sex-specific DNA methylation may help establish sex differences in expression and function and that sex-specific alterations in DNA methylation in response to exposures could contribute to sex-variable toxicological responses.

Background: Newer intravenous lipid emulsions (ILEs), such as fish oil-based intravenous lipid emulsions (FO-ILEs) and soybean oil, medium-chain triglycerides, olive oil, and fish oil-based intravenous lipid emulsions (SMOF-ILEs), provide alternatives to soybean oil-based intravenous lipid emulsions (SO-ILEs). We explored current ILE practice patterns among intestinal rehabilitation and transplant centers., Methods: A survey was developed addressing ILE availability, ILE preference in clinical scenarios, and factors influencing ILE choice. This survey was reviewed locally and by the North American Society for Pediatric Gastroenterology, Hepatology, and Nutrition Intestinal Rehabilitation Special Interest Group, the Intestinal Rehabilitation and Transplant Association scientific committee, and the American Society of Parenteral and Enteral Nutrition pediatric intestinal failure section research committee. We recruited providers nationally and internationally from centers with and without intestinal transplant programs., Results: We included 34 complete responses, 29 from the United States. Sixteen centers performed intestinal transplants. All centers had access to SMOF-ILEs, 85% had access to FO-ILEs, and 91% had access to SO-ILEs. In new patients, 85% use SMOF-ILEs as the first choice ILE. In those with new intestinal failure-associated liver disease (IFALD), FO-ILE was preferred to SMOF-ILE (56% vs 38%). In those developing IFALD on SMOF-ILE, 65% switched to FO-ILE, whereas 24% remained on SMOF-ILE., Conclusions: Centers have routine access to alternative ILEs, and these are quickly replacing SO-ILEs in all circumstances. Future work should focus on how this shift in practice affects outcomes to provide decision support in specific clinical scenarios., (© 2022 American Society for Parenteral and Enteral Nutrition.)

We report a precise measurement of the parity-violating (PV) asymmetry A&#95;{PV} in the elastic scattering of longitudinally polarized electrons from ^{48}Ca. We measure A&#95;{PV}=2668±106(stat)±40(syst) parts per billion, leading to an extraction of the neutral weak form factor F&#95;{W}(q=0.8733  fm^{-1})=0.1304±0.0052(stat)±0.0020(syst) and the charge minus the weak form factor F&#95;{ch}-F&#95;{W}=0.0277±0.0055. The resulting neutron skin thickness R&#95;{n}-R&#95;{p}=0.121±0.026(exp)±0.024(model)  fm is relatively thin yet consistent with many model calculations. The combined CREX and PREX results will have implications for future energy density functional calculations and on the density dependence of the symmetry energy of nuclear matter.

Competing Interests: The author declares that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

We report precision determinations of the beam-normal single spin asymmetries (A&#95;{n}) in the elastic scattering of 0.95 and 2.18 GeV electrons off ^{12}C, ^{40}Ca, ^{48}Ca, and ^{208}Pb at very forward angles where the most detailed theoretical calculations have been performed. The first measurements of A&#95;{n} for ^{40}Ca and ^{48}Ca are found to be similar to that of ^{12}C, consistent with expectations and thus demonstrating the validity of theoretical calculations for nuclei with Z≤20. We also report A&#95;{n} for ^{208}Pb at two new momentum transfers (Q^{2}) extending the previous measurement. Our new data confirm the surprising result previously reported, with all three data points showing significant disagreement with the results from the Z≤20 nuclei. These data confirm our basic understanding of the underlying dynamics that govern A&#95;{n} for nuclei containing ≲50 nucleons, but point to the need for further investigation to understand the unusual A&#95;{n} behavior discovered for scattering off ^{208}Pb.

Cannabis use alters sperm DNA methylation, but the potential reversibility of these changes is unknown. Semen samples from cannabis users and non-user controls were collected at baseline and again following a 77-day period of cannabis abstinence (one spermatogenic cycle). Users and controls did not significantly differ by demographics or semen analyses. Whole-genome bisulfite sequencing identified 163 CpG sites with significantly different DNA methylation in sperm between groups ( P  < 2.94 × 10 -9 ). Genes associated with altered CpG sites were enriched with those involved in development, including cardiogenesis and neurodevelopment. Many of the differences in sperm DNA methylation between groups were diminished after cannabis abstinence. These results indicate that sustained cannabis abstinence significantly reduces the number of sperm showing cannabis-associated alterations at genes important for early development., (© The Author(s) 2021. Published by Oxford University Press.)

Thin elastic films can spontaneously attach to liquid interfaces, offering a platform for tailoring their physical, chemical, and optical properties. Current understanding of the elastocapillarity of thin films is based primarily on studies of planar sheets. We show that curved shells can be used to manipulate interfaces in qualitatively different ways. We elucidate a regime where an ultrathin shell with vanishing bending rigidity imposes its own rest shape on a liquid surface, using experiment and theory. Conceptually, the pressure across the interface "inflates" the shell into its original shape. The setup is amenable to optical applications as the shell is transparent, free of wrinkles, and may be manufactured over a range of curvatures.

DNA methylation is an important epigenetic mechanism involved in proper genome function. Bisulfite pyrosequencing (PSQ) is a commonly used technique to quantify DNA methylation. Although very accurate, bisulfite pyrosequencing can be expensive and time consuming for large-scale quantitative DNA methylation analysis at the single nucleotide level. High throughput DNA methylation sequencing has the potential to address these limitations, but its comparability to other methylation detection methods has not been well studied. We compared QIAseq Targeted Methyl Panel technologies (QMS) and PSQ by analyzing four CpG sites within four genes involved in neurodevelopment . QMS and PSQ had an average 5.6% difference in the detected level of DNA methylation for the same four CpG sites. However, we observed a strong correlation in the levels of methylation across all four CpG sites between the two technologies. These findings demonstrate the comparability of QMS relative to PSQ in the ability to accurately quantify DNA methylation at specific CpG sites., (Copyright: © 2021 by the authors.)

Background: Recent studies suggest that intermittent fasting or skipping breakfast may be good strategies for weight loss and better health. The objective of this study was to determine whether regular breakfast is associated with overall or cardiovascular mortality., Methods: Cohort study with follow-up mortality data from the NHANES 1999-2002. National weighted sample. Outcomes were overall and cardiovascular mortality; secondary was fiber intake., Results: Out of 5761 participants, there were 4778 (82.9%) identified as breakfast eaters and 2027 deaths (35.2%); 469 (23.1%) deaths were due to cardiovascular diseases. The average daily intake of calories was 2015, and fiber was 16.3 g/day. A total of 17.7%, 66.0%, and 11.4% of participants had diabetes, hypertension, and cardiovascular diseases, respectively. Analysis showed breakfast eaters were older, had lower body mass index, and ate more calories and fiber daily than non-breakfast eaters. Cox proportional hazard regression analyses showed that compared to non-breakfast eaters, the breakfast eaters were less likely to experience mortality after multivariable adjustments (overall mortality: hazard ratio [HR], 0.69; 95% confidence interval [CI], 0.57-0.84 and cardiovascular mortality: HR, 0.45; 95% CI, 0.32-0.63). For the breakfast eaters, fiber intake >25 g/day was associated with 21% (HR, 0.79; 95% CI, 0.66-0.96) reduction in all-cause mortality after multivariable adjustments., Conclusions: Regular daily intake of breakfast appears to be associated with lower overall and cardiovascular mortality, particularly when consuming fiber >25 g/day. Further studies examining specific breakfast foods and the timing of foods would be helpful., Competing Interests: Conflict of interest: There are no conflicts of interest associated with this study or manuscript., (© Copyright 2021 by the American Board of Family Medicine.)

We report a precision measurement of the parity-violating asymmetry A&#95;{PV} in the elastic scattering of longitudinally polarized electrons from ^{208}Pb. We measure A&#95;{PV}=550±16(stat)±8(syst) parts per billion, leading to an extraction of the neutral weak form factor F&#95;{W}(Q^{2}=0.00616  GeV^{2})=0.368±0.013. Combined with our previous measurement, the extracted neutron skin thickness is R&#95;{n}-R&#95;{p}=0.283±0.071  fm. The result also yields the first significant direct measurement of the interior weak density of ^{208}Pb: ρ&#95;{W}^{0}=-0.0796±0.0036(exp)±0.0013(theo)  fm^{-3} leading to the interior baryon density ρ&#95;{b}^{0}=0.1480±0.0036(exp)±0.0013(theo)  fm^{-3}. The measurement accurately constrains the density dependence of the symmetry energy of nuclear matter near saturation density, with implications for the size and composition of neutron stars.

Objectives: Describe clinical characteristics, management, and outcome in a cohort of megacystis microcolon intestinal hypoperistalsis syndrome (MMIHS) patients., Methods: We conducted a retrospective chart review of MMIHS patients followed at a large transplant and intestinal rehabilitation center over a period of 17 years., Results: We identified 25 patients with MMIHS (68% girls, 13 transplanted). One transplanted and 1 nontransplanted patient were lost to follow-up. We estimated 100, 100, and 86% for 5-, 10-, and 20-year survival, respectively, with only 1 death. Of the 22 patients alive at the time of study (11 transplanted, 11 nontransplanted), median age was 9.2 years (range 2.7-22.9 years). Longest posttransplant follow-up was 16 years. Seventeen patients had available prenatal imaging reports; all showed distended bladder. Eight had genetic testing (5, ACTG2; 2, MYH11; 1, MYL9). Almost all patients had normal growth with median weight z-score -0.77 (interquartile range -1.39 to 0.26), height z score -1.2 (-2.04 to -0.48) and body mass index z-score 0.23 (-0.37 to 0.93) with no statistical difference between transplanted and nontransplanted patients. All nontransplanted patients were on parenteral nutrition with minimal/no feeds, and all except 1 of the transplanted patients were on full enteral feeds. Recent average bilirubin, INR, albumin, and creatinine fell within the reference ranges., Conclusions: This is the largest single-center case series with the longest duration of follow-up for MMIHS patients. In the current era of improved intestinal rehabilitation and transplantation, MMIHS patients have excellent outcomes in survival, growth, and liver function. This observation contradicts previous reports and should alter counselling and management decisions in these patients at diagnosis., Competing Interests: The authors report no conflicts of interest., (Copyright © 2020 by European Society for Pediatric Gastroenterology, Hepatology, and Nutrition and North American Society for Pediatric Gastroenterology, Hepatology, and Nutrition.)