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1. High throughput and rapid isolation of extracellular vesicles and exosomes with purity using size exclusion liquid chromatography

Author

Kshipra S. Kapoor, Kristen Harris, Kent A. Arian, Lihua Ma, Beatriz Schueng Zancanela, Kaira A. Church, Kathleen M. McAndrews, and Raghu Kalluri

Subjects
Extracellular vesicles, Size exclusion - fast performance liquid chromatography, Isolation methods, Materials of engineering and construction. Mechanics of materials, TA401-492, Biology (General), QH301-705.5
Abstract

Extracellular vesicles (EVs) have emerged as potential biomarkers for diagnosing a range of diseases without invasive procedures. Extracellular vesicles also offer advantages compared to synthetic vesicles for delivery of various drugs; however, limitations in segregating EVs from other particles and soluble proteins have led to inconsistent EV retrieval rates with low levels of purity. Here, we report a new high-yield (88.47 %) and rapid (

Published
2024
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