Search

Feeding habits of Yellowfin Seabream (Acanthopagrus latus) was investigated in coastal waters of the Northern Persian Gulf. This investigation was conducted by monthly sampling of thirty fish from September 2011 through August 2012. Fish size ranged from 17.98 ± 2.07 to 32.31 ± 6.52 cm in total length and from 134.01 ± 45.62 to 720.46 ± 292.58 g in weight. The highest value of gastro-somatic index was obtained in September (5.22 ± 0.04) and the lowest in December (1.61 ± 0.03) with annual average of 2.50 ± 0.60. The result of gastro-somatic index revealed that the highest feeding activity of A. latus was during autumn. The highest level of vacuity index was observed in summer (34.95 ± 4.71) and the lowest in autumn (25.88 ± 2.71) indicating that the highest number of empty stomachs was in summer. Annual average of vacuity index was 30.14 ± 5.72 exhibiting that A. latus was comparatively gluttonous in the Northern Persian Gulf. Bivalves and shrimps were the major food items found in the stomach of A. latus showing food preference indices of 45.86% and 30.67%, respectively. Other food items included crabs (12.66%), aquatic plants (4.05%), animal derivatives (4.52%) and gastropods (2.23%). According to the results, animal derivatives, aquatic plants and gastropods were eaten accidentally and were not the food items of A. latus in coastal waters of Hormozgan. The average relative length of gut was 1.41 ± 0.15 showing that A. latus was omnivorous in this region.

The relationships between somatic growth and otolith dimensions, otolith size to estimated age and growth parameters of the tigertooth croaker ( Otolithes ruber) were investigated in 100 specimens (size range: 19.1-52.0 cm, total length) from the Oman Sea area, September 2014. All 100 otoliths were sectioned and determined by age. The oldest specimen was a 4.5-year-old female with a total length of 40.6 cm; the youngest specimen was also a female estimated at 1 year of age with a total length of 19.1 cm. The von Bertalanffy growth equation was estimated as Lt = 54.70 (1 − exp (−0.37 ( t + 0.21))). Concluded was that there is a significant relationship between body size, otolith dimensions and estimated age of Otolithes ruber. [ABSTRACT FROM AUTHOR]

In this study, the reproductive biology of female freshwater crab Sodhiana iranica , oogenesis and ovarian development were described. An H-shaped ovary consisting of a pair of long ovarian sacs connected by a narrow bridge tube was located in the cephalothorax on the dorsal side of the stomach. Females at different stages of ovarian development were anesthetized and their ovaries were removed, photographed, fixed, and processed for histological examination. Based on the light microscopic observations of cells’ sizes, chromatin patterns, and amount of lipid vesicles, the female germ cells could be classified into seven different stages: (1) oogonia (Oog), (2) primary oocytes (pOc), (3) early previtellogenic oocyte (Oc1), (4) late previtellogenic oocyte(Oc2), (5) early vitellogenic oocyte (Oc3), (6) late vitellogenic oocyte (Oc4), and (7) mature oocyte (mOc). Oog are small oval-shaped cells with irregular-shaped nuclei. Oog undergo first meiotic division to become primary oocytes. The primary oocytes are small oval-shaped cells with large nuclei. The secondary oocytes derived from 2nd meiosis and comprise five steps. Four ovarian development stages were found for females based on the number and types of oocytes present in each stage: spent I (Spent), II (Proliferative) and III (Premature) and stage IV (Mature). The ovaries, macroscopically, varied in size and color during each developmental stage and, microscopically, the ovarian stages differed in proportion oogonia, and the secondary oocytes. During ovarian stage I, ovary contains primarily oogonia, primary oocytes and Oc1. In stage II, contains mainly Oc1, Oc2, and Oc3, while in stage III the predominant cells are Oc4. Mature oocytes appear synchronously in stage IV. [ABSTRACT FROM AUTHOR]

Summary This study presents some traits of the Aphanius ginaonis life history in the Geno hot spring and explains the potential risks of its extinction. Sampling was from March 2009 to February 2010. A total of 61 males and 71 females were measured (total length) and weighed, with data on reproductive biometry also taken. Growth parameters were determined in addition to weight-length relationships: W ( t) = 0.012TL3.42, R2 = 0.96 for females and W ( t) = 0.0101TL3.38, R2 = 0.94 for males. A. ginaonis females showed an asymptotic total length (TL∞) of 53.03 mm; the growth coefficient, K (year−1) 0.15, t0 (year) 1.01; and natural mortality coefficient M (year−1) 0.62. In males the value for TL∞ was 48.83 mm; for K (year−1) 0.2, t0 (year) 0.44; and M (year−1) 0.49. The relationship between absolute fecundity and fish size (total length, body weight or age) showed a strong correlation to body weight. The A. ginaonis population is threatened with extinction - and a co-management in cooperation with the local population for strong protection measures is urgently needed. [ABSTRACT FROM AUTHOR]

Summary: In this study, length‐length relationships (LLRs) and weight‐length relationships (WLRs) were established for a Carangidae species: Uraspis helvola and tow Flatfishes: Pseudorhombus elevatus and Psettodes erumei that are substantial marine fish species to commercial fisheries in Persian Gulf that are indigenous marine fish species in the region. Samples were collected by non‐selective fish bottom trawler with stretch mesh size of 4 centimeters at the cod‐end area, which helped us to obtain such a broad range size that included small, medium and large size classes during June to August 2017. Hitherto, the length‐weight model presented in this study for Pseudorhombus elevatus and Uraspis helvola were not documented on Fishbase. Additionally, new records of maximum length are presented as 64.5 and 30 centimeters for P. erumei and P. elevatus respectively. A comprehensive size distribution and highly significant fitted model (r2>0.95) in this research provides useful insight for future studies on fish biology, fisheries assessment and ecological modeling. [ABSTRACT FROM AUTHOR]

The length-weight relationships ( LWRs) for four little-known shark species collected in the Persian Gulf and Oman Sea from March 2014 to September 2015 are presented, namely: Carcharhinus dussumieri, Carcharhinus macloti, Chiloscyllium arabicum, and Chaenogaleus macrostoma. [ABSTRACT FROM AUTHOR]

Length-weight relationships ( LWRs) for four little-known fish species collected in the northern Persian Gulf (south of Iran) are presented, namely, Leiognathus lineolatus, Grammoplites suppositus, Eupleurogrammus muticus and Acanthocepola abbreviate. Thus far, these are the first LWRs for these species in the international scientific literature. [ABSTRACT FROM AUTHOR]

Length–weight relationships, W = aL b, were calculated for three species of mudskippers: Scartelaos tenuis, Periophthalmus waltoni and Boleophthalmus dussumieri from the Iranian coast of the Persian Gulf. Values of b ranged from 2.10 to 2.86 for both sexes at seven sites for the three species. [ABSTRACT FROM AUTHOR]

Approximately 80% of luminous organisms live in the oceans, and considerable diversity of life dependence on bioluminescence has been observed in marine organisms. Among vertebrates, luminous fish species are the only group of vertebrates that have the ability to emit bioluminescent light. Meanwhile, the lantern fish family (Myctophidae), with 33 genera all of which have the ability to emit light, is considered the most prominent family among the luminous fish of the deep oceans and seas. Lantern fish Benthosema pterotum has bioluminescence properties due to the presence of photophores scattered in its ventral-lateral region. However, no research has been performed on its bioluminescence system and light emission mechanism. The present research aimed to assess the type of bioluminescence, pigment, photoprotein, or luciferin-luciferase system in B. pterotum. In order to determine the type of light-emitting system in B. pterotum species, several specific experiments were designed and performed. It was shown that the light emission system in B. pterotum species is categorized into the luciferin-luciferase type. Conducting this research was not only innovative, but it also could be the beginning of further research in the field of marine biochemistry and production of the recombinant active forms of enzymes for industrial, commercial, medical, and pharmaceutical purposes., (© 2023. The Author(s), under exclusive licence to Springer Nature B.V.)

Conventional drug delivery systems are challenged by concerns related to systemic toxicity, repetitive doses, drug concentrations fluctuation, and adverse effects. Various drug delivery systems are developed to overcome these limitations. Nanomaterials are employed in a variety of biomedical applications such as therapeutics delivery, cancer therapy, and tissue engineering. Physiochemical nanoparticle assembly techniques involve the application of solvents and potentially harmful chemicals, commonly at high temperatures. Genetically engineered organisms have the potential to be used as promising candidates for greener, efficient, and more adaptable platforms for the synthesis and assembly of nanomaterials. Genetically engineered carriers are precisely designed and constructed in shape and size, enabling precise control over drug attachment sites. The high accuracy of these novel advanced materials, biocompatibility, and stimuli-responsiveness, elucidate their emerging application in controlled drug delivery. The current article represents the research progress in developing various genetically engineered carriers. Organic-based nanoparticles including cellulose, collagen, silk-like polymers, elastin-like protein, silk-elastin-like protein, and inorganic-based nanoparticles are discussed in detail. Afterward, viral-based carriers are classified, and their potential for targeted therapeutics delivery is highlighted. Finally, the challenges and prospects of these delivery systems are concluded., (© 2022 The Authors. Advanced Healthcare Materials published by Wiley-VCH GmbH.)

The present study focused on the cloning, expression, and characterization of L-asparaginase of marine Pseudomonas aeruginosa HR03 isolated from fish intestine. Thus, a gene fragment containing the L-asparaginase sequence of Pseudomonas aeruginosa HR03 isolated from the fish intestine was cloned in the pET21a vector and then expressed in Escherichia coli BL21 (DE3) cells. Thereafter, the recombinant L-asparaginase (HR03Asnase) was purified by nickel affinity chromatography, and the enzymatic properties of HR03Asnase, including the effects of pH and temperature on HR03Asnase activity and its kinetic parameters, were determined. The recombinant enzyme HR03Asnase showed the highest similarity to type I L-asparaginase from Pseudomonas aeruginosa. The three-dimensional (3D) modeling results indicate that HR03Asnase exists as a homotetramer. Its molecular weight was 35 kDa, and the maximum activity of the purified enzyme was observed at pH8 and at 40 °C. The k m and V max of the enzyme obtained with L-asparagine as substrate were 10.904 mM and 3.44 × 10 -2  mM/min, respectively. The maximum activity of HR03Asnase was reduced by 50% at 90 °C after 10-min incubation; however, the enzyme maintained more than 20% of its activity after 30-min incubation. This enzyme also maintained almost 50% of its activity at pH 12 after 40-min incubation. The evaluation of pH and temperature stability of HR03Asnase showed that the enzyme has a wide range of activity, which is a suitable characteristic for its application in different industries. Overall, the results of the present study indicate that marine sources are promising biological reservoirs for enzymes to be used for biotechnological purposes, and marine thermostable HR03Asnase is likely a potential candidate for its future usage in the pharmaceutical and food industries., (© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

Despite the increasing interest in mangroves as one of the most carbon-rich ecosystems, arid mangroves are still poorly investigated. We aimed to improve the knowledge of biomass and soil carbon sequestration for an arid mangrove forest located at the Azini creek, Sirik, Hormozgan Province (Iran). We investigated the biomass and organic carbon stored in the above and belowground biomass for three different regions selected based on the composition of the principal species: (1) Avicennia marina, (2) mixed forest of A. marina and Rhizophora mucronata, and (3) R. mucronata. Topsoil organic carbon storage to 30 cm depth was also estimated for each analyzed area. Biomass carbon storage, considering both aboveground (AGB) and belowground biomass (BGB), was significantly different between the cover areas. Overall, the mean forest biomass (MFB) was 283.1 ± 89 Mg C ha -1 with a mean C stored in the biomass of 128.9 ± 59 Mg C ha -1 . Although pure Rhizophora stand showed the lowest value of above and below tree carbon (AGC + BGC); 17.6 ± 1.9 Mg C ha -1 ), soil organic carbon stock in sites under Rhizophora spp. was significantly higher than in the site with pure stand of Avicennia spp. Overall, forest soil stored the highest proportion of Sirik mangrove ecosystem organic carbon (59%), with a mean value of 188.3 ± 27 Mg C ha -1 . These results will contribute to broaden the knowledge and the dataset available, reducing the uncertainties related to estimates and modeling of carbon pools in arid mangrove ecosystem, which also represent an important climatic threshold of mangrove worldwide distribution., (© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Early prediction of patient mortality risks during a pandemic can decrease mortality by assuring efficient resource allocation and treatment planning. This study aimed to develop and compare prognosis prediction machine learning models based on invasive laboratory and noninvasive clinical and demographic data from patients' day of admission. Three Support Vector Machine (SVM) models were developed and compared using invasive, non-invasive, and both groups. The results suggested that non-invasive features could provide mortality predictions that are similar to the invasive and roughly on par with the joint model. Feature inspection results from SVM-RFE and sparsity analysis displayed that, compared with the invasive model, the non-invasive model can provide better performances with a fewer number of features, pointing to the presence of high predictive information contents in several non-invasive features, including SPO2, age, and cardiovascular disorders. Furthermore, while the invasive model was able to provide better mortality predictions for the imminent future, non-invasive features displayed better performance for more distant expiration intervals. Early mortality prediction using non-invasive models can give us insights as to where and with whom to intervene. Combined with novel technologies, such as wireless wearable devices, these models can create powerful frameworks for various medical assignments and patient triage., Competing Interests: The authors have declared that no competing interests exist.

Mangroves are ideal habitat for a variety of marine species especially brachyuran crabs as the dominant macrofauna. However, the global distribution, endemicity, and latitudinal gradients of species richness in mangrove crabs remains poorly understood. Here, we assessed whether species richness of mangrove crabs decreases towards the higher latitudes and tested the importance of environmental factors such as Sea Surface Temperature (SST) in creating the latitudinal gradients in species richness of mangrove crabs. A total of 8262 distribution records of 481 species belonging to six families of mangrove crabs including Camptandriidae, Dotillidae, Macrophthalmidae, Ocypodidae, Sesarmidae, and Oziidae were extracted from open-access databases or collected by the authors, quality controlled, cleaned, and analyzed. Species richness was plotted against 5° latitudinal bands in relation to environmental factors. The R software and ArcGIS 10.6.1 were used to analyze the species latitudinal range and richness as well as to map the distribution of mangrove forest, endemic species, species geographical distribution records, and biogeographic regions. The Indo-West Pacific showed the highest species richness of mangrove crabs where more than 65% of species were found in the Indian Ocean and along the western Pacific Ocean. Our results showed that there are 11 significantly different biogeographic regions of mangrove crabs. The highest endemicity rate was observed in the NW Pacific Ocean (29%). Latitudinal patterns of species richness in Macrophthalmidae, Ocypodidae, and Sesarmidae showed an increasing trend from the poles toward the intermediate latitudes including one dip near the equator. However, latitudinal gradients in Camptandriidae, Dotillidae, and Oziidae were unimodal increasing from the higher latitudes towards the equator. Species richness per 5° latitudinal bands significantly increased following mean SST mean (°C), calcite, euphotic depth (m), and mangrove area (km 2 ) across all latitudes, and tide average within each hemisphere. Species richness significantly decreased with dissolved O 2 (ml l -1 ) and nitrate (μmol l -1 ) over all latitudes and in the southern hemisphere. The climax of global latitudinal species richness for some mangrove was observed along latitudes 20° N and 15°-25° S, not at the equator. This can suggest that temperature is probably the key driver of latitudinal gradients of mangrove crabs' species richness. Species richness and mangrove area were also highly correlated., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

Cryopreservation is the most effective method for preserving semen for a long period of time. However, during the freeze-thaw process, production of reactive oxygen species (ROS) leads to a steep reduction in sperm fertility indices. In this study, we tested the effects of the extract of the coelomic cavity of five Holotheria parva, a marine organism rich in antioxidants, for its ROS-scavenging activity and cryoprotective effects on oxidative stress. Using a total of 50 semen samples, our results demonstrated that doses of 250 and 500 µg/ml of H. parva coelomic cavity extract significantly increased sperm vitality as compared to the control (p < .05). The addition of 250 µg/ml of the extract exerted a significant positive effect on sperm motility. Moreover, sperm DNA damage and ROS production were significantly reduced at extract concentrations of 250 and 500 µg/ml (p < .05). To the best of our knowledge, the results of this study represent the first demonstration of the possibility of improving sperm parameters and reducing ROS production and DNA damage by supplementing sperm freezing media with H. parva coelomic extract. Our results suggested that H. parva coelomic extract could be useful for improving the fertilising ability of frozen-thawed human semen., (© 2020 Blackwell Verlag GmbH.)

A smart contact lens can be used as an excellent interface between the human body and an electronic device for wearable healthcare applications. Despite wide investigations of smart contact lenses for diagnostic applications, there has been no report on electrically controlled drug delivery in combination with real-time biometric analysis. Here, we developed smart contact lenses for both continuous glucose monitoring and treatment of diabetic retinopathy. The smart contact lens device, built on a biocompatible polymer, contains ultrathin, flexible electrical circuits and a microcontroller chip for real-time electrochemical biosensing, on-demand controlled drug delivery, wireless power management, and data communication. In diabetic rabbit models, we could measure tear glucose levels to be validated by the conventional invasive blood glucose tests and trigger drugs to be released from reservoirs for treating diabetic retinopathy. Together, we successfully demonstrated the feasibility of smart contact lenses for noninvasive and continuous diabetic diagnosis and diabetic retinopathy therapy., (Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).)

The monooxygenase enzymes, cytochrome P450s (CYPs), are ubiquitous in their presence and versatile in their functions. They are indispensable for hormone synthesis, dietary metabolism, and detoxification among other roles. CYPs from marine organisms are interesting candidates for recruitment in drug biotransformation, synthesis of therapeutics, bioconversion of xenobiotics, degradation of chemical carcinogens, and for use as ecotoxicological tools. However, excess CYP production in marine organisms indicates a 'stressed condition', which has a negative impact on the ecological balance. This review discusses the CYPs in marine organisms of various phyla, their application potentials, as well as the need to restore normal CYP level in them to regulate the relationship between marine organisms and environment., (Copyright © 2019 Elsevier B.V. All rights reserved.)

Despite the fact that magnetic iron oxide nanoparticles (Fe 3 O 4 -MNPs) considered as the most promising nanoparticles (NPs) in biomedicine and environmental biotechnology, their safety and ecotoxicological impacts of biogenic and chemogenic routes of Fe 3 O 4 -MNPs in the marine aquatic system is scarcely studied. In this work, we report the optimized and suitable phyco-synthesis route for nano-Fe 3 O 4 based on the six selected species of the Persian Gulf seaweeds: Ulva prolifera, U. flexuosa, U. linza, U. intestinalis, U. clathrata, and Sargassum boveanum. Moreover, antibacterial activities and acute zooplanktonic responses in Artemia salina and acorn barnacle Amphibalanus amphitrite to chemogenic and biogenic Fe 3 O 4 -MNPs, were evaluated. Although all the seaweeds extract showed reducing potential for Fe 3 O 4 -MNPs green synthesis - mainly on the basis of characterization results- the algal route selectivity has been demonstrated to be important for the biosynthesis of magnetite NPs. Herein, the cubo-spherical and polydisperse U. prolifera-derived Fe 3 O 4 -MNPs with particles sizes of 9.59 nm were the best ones. The comparative zooplanktonic cytotoxicity of chemo- and bio-route of Fe 3 O 4 -MNPs exhibited no acute toxicity in nauplii and adults of A. salina (96-h EC 50  ≥ 1000 mg/L) and the potential of toxicity in A. amphitrite nauplii (48-h EC 50  = 466.5 and 842.3 mg/L for chemo- and bio-route of Fe 3 O 4 -MNPs, respectively). The in vitro antimicrobial activity of both chemo- and bio-route of magnetite NPs to selective human pathogenic bacteria and fungi (i.e. n = 11) showed strong antagonistic activity against Staphylococcus epidermidis, Bacillus subtilis, B. pumulis, and Saccharomyces cerevisiae. In conclusion, these findings demonstrate the optimized phyco-fabrication of Fe 3 O 4 -MNPs as promising nontoxic approach in ecobiotechnology, the new insight about the potential adverse effects of chemosynthesized Fe 3 O 4 -MNPs to crustacean zoo-organisms after their possible entrance into the marine environments, and bio/chemo-route Fe 3 O 4 -MNPs as pivotal agent for nanoantimicrobials., (Copyright © 2019. Published by Elsevier B.V.)

Following the recent progress in magnetic nanotechnology, concern over the optimal benefits and potential risks of iron oxide nanoparticles (Fe NPs), has increased. Hence, to minimize the negative impacts of inorganic Fe NPs, we report the phyco-synthesis and characterization of superparamagnetic Fe 3 O 4 NPs via reduction of ferric/ferrous chloride solution (2:1 M ratio; 88 °C) with green macroalga, Ulva flexuosa (wulfen) J.Agardh aqueous extract. The biogenic process is clean, eco-friendly, rapid, and facile to handle. These green fabricated magnetite NPs are characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM), field emission scanning electron microscopy (FESEM), thermogravimetric analysis (TGA), particle size analysers (PSA), zeta potential (ZP) measurement, and vibrating sample magnetometry (VSM) techniques. The results confirmed that the cubo-spherical, polydisperse of biosynthesized Fe 3 O 4 NPs with an average diameter of 12.3 nm was formed. The antagonistic effects of algal extract, chemo-route and U. flexuosa-derived Fe 3 O 4 MgNPs on selective human pathogenic microbes (i.e. n = 11) resulted in strong antibacterial and moderate antifungal activity. The comparative toxic and reproductive effects of the chemo- and bio-routes of Fe 3 O 4 -MgNPs against rotifer B. rotundiformis exhibited low acute toxicity with a lower inducing effect of biogenic nano-magnetite on reduction rotifer reproductive rate than its chemogenic counterpart. In view of the nanoecotoxicity, though the current study covered a wide range of exposure concentrations (10-500 mg/L) of organic and inorganic nano sizes of Fe 3 O 4 in brackish water rotifer, a biotoxicity assay at higher dosage or a comprehensive risk assessment in different aqua-organisms is recommended., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

Superoxide dismutase (SOD) is the pivotal antioxidant enzyme that defends organisms against the oxidative stresses of superoxide radicals. In this experimental study, purification of SOD from the leaves of Avicennia marina (grey mangrove or white mangrove) from the family Acanthaceae, located in Sirik mangrove forest on the shore of the Gulf of Oman was performed, for the intended characterization of SOD. The Sirik AmSOD (A. marina SOD) expressed optimum activity in the pH range of 6-9 with the maximum activity at pH 8. The optimal temperature for Sirik AmSOD activity was 70°C. Comparison of the pH and temperature optima in two regions (the Persian Gulf and the Gulf of Oman) showed significant differences with P<0.05. The SOD from the Persian Gulf was more resistant against the environmental stressors, because of the biochemical adaption to this environment, which is harsher. The evidence from these results suggests that AmSOD has different characteristics in each place, and mangroves undergo different adaptations and require different protections. The results of the enzymatic research can be useful for ecological management of organisms., (Copyright © 2018 Elsevier Inc. All rights reserved.)

This study aimed to investigate the ecological risk assessment of Khamir, Tiyab, and Jagin estuaries and the impact of anthropogenic activities on these ecosystems during a one-year study period (April 2015 to March 2016) using trace metals as pollution indices. The sediment samples were collected from nine sampling stations, following a gradient of contamination from the industrial wastewater and shrimp farming effluents to the less impacted stations. Pollution indices (i.e. PERI and PLI) were applied to ascertain the sediment quality. Based on pollution indices, the overall pattern of environmental quality status demonstrated that industrial sewage and shrimp farming effluents are major sources of pollution in the Khamir and Tiyab estuaries, respectively. The sediments in the most stations in studied coastal ecosystems of Iran posed moderate or considerable ecological risk. Results from this study showed that Zn, Pb, Cu, and Cd were mostly derived from anthropogenic activities such as domestic sewage and industrial effluents. Also, the findings of this study revealed that the pollution indices are suitable for evaluating the environmental situation of coastal ecosystems and the separation of areas with less impacted by human activities from areas affected by these activities and could be used as a robust management tool for monitoring programs in coastal areas. Altogether, these findings could be useful in providing more effective and targeted strategies of development better management practices for coastal areas., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

A novel Cu/Zn-superoxide dismutase was extracted from Avicennia marina and purified. The sample was collected from Khamir port located in the north shore of Persian Gulf. The purification procedure comprised of (NH 4 ) 2 SO 4 precipitation followed by CM-Sephadex C-50 and DEAE-Sepharose chromatography, and gel filtration chromatography (Sephadex G-75). The enzyme with a characteristic molecular weight of 31kDa, measured by SDS-page, showed its highest catalytic efficiency at pH 8.0 and 50°C. Its activity was greatly inhibited by cyanide and hydrogen peroxide. The pH profile showed that the enzyme could maintain most of its activity at pH values ranging from 5 to 10. The temperature profile of this enzyme showed a broad range of activity compared with other superoxide dismutases. Catalytic hydrolysis rate followed Michaelis-Menten equation. The values of k cat and K m were obtained from Michaelis-Menten plot as 107000s -1 and 11.5μmol respectively. The evidences from kinetic and thermodynamic parameters suggest that Avicennia marina superoxide dismutase (AmSOD) can be used as a suitable enzyme for biotechnological and pharmacological applications., (Copyright © 2017 Elsevier B.V. All rights reserved.)

Freshwater crab, Sodhiana iranica, is an endemic gecarcinucid crab that has been recently reported from Southern Iran. This research examined some reproductive aspects of S. iranica from Eelood freshwater spring, Southern Iran. Crabs were haphazardly sampled from April 2012 to April 2013, on a bimonthly basis. Measurements of Gonado-Somatic Index (GSI), Hepato-Somatic Index (HSI), oocyte diameter, and other aspects such as carapace width (CW) and total body weight (TW) were made in the captured specimens. Results showed a single seasonal reproductive cycle. Maturation and spawning occurred from December 2012 to April 2013 during the study period. The peaks of HSI were observed in April 2012 and February 2013. The oocyte diameter showed its most significant increase between August 2012 and February 2013. Considering the single seasonal breeding of S. iranica, a correct management, during the reproductive cycle, is necessary to support a healthy stock of this crab.

A study was carried out to investigate the daily rhythms of locomotor and feeding activity of Khajoo, Schizothorax pelzami, a candidate species for freshwater aquaculture. Using self-feeder juvenile Khajoo were exposed to a 12/12 LD cycle to determine the rhythms of locomotor and feeding activity. The effects of feeding on locomotor and feeding activity of fish were also examined. Finally, the endogenous rhythmicity under different lighting condition tested. Fish displayed a strictly diurnal feeding and locomotor activities with 98% and 84% of the total activity occurred in the photophase, respectively. In scheduled feeding, both the L-group (fed in light) and the D-group (fed in the dark) showed a diurnal locomotor activity pattern. However, the L-group had a peak of locomotor activity near the feeding time, but the D-group had a scarce locomotor activity in the scatophase with no significant change at the mealtime. Most of the individuals display free-running rhythms when exposed to different lighting condition including, constant darkness, ultradian 45:45 min LD cycle and reversed DL photo cycle. Taken together the results of this study showed that both locomotor and feeding activity have diurnal rhythms in Khajoo S. pelzami, even fish feeding had taken place at night. Additionally, the free-running locomotor activity of the fish in the absence of external light stimuli, suggests the existence of an endogenous timing mechanism in this fish species.

Background: The widespread use of chemical insecticides, resistance in vectors and environmental problems, all have led to an increased interest in the use of biological agents in malaria control programs. The most important functional elements are the native fish. The aim of this study was to identify the native species of lavivorous fish in Rudan County, southern Iran, to introduce an effective species and to propose its' implementation in the national malaria control program., Methods: This ecologically descriptive study was conducted during 2011-2012 using random sampling from different fish habitats of Rudan County. The shoals of fish were caught using fishing net. Fish samples were then identified in the Ichthyology lab, Department of Fisheries and the Environment, Hormozgan University., Results: Three species of larvivorous fish were identified as follows: Gambusia holbrooki, Aphaniusdispar dispar and Aphanius sp. The latter species has the most distribution in the study area and needs more morphological and molecular studies for identification at the species level., Conclusion: Two species of native fish, i.e., A. dispar and A. sp. with larvivorous potential live in the area. Further studies on their predatory property are recommended in order to apply this local potential against malaria vectors in the area.

The ability of marine organism to cope with oxidative stress is one of the main factors that influence its survival in the marine environment, when senescence conditions prevail. The antioxidative defense system includes enzymatic and non-enzymatic components. Among the enzymatic system, superoxide dismutases are the first and most important of the antioxidant metalloenzymes. Four different types of metal centers have been detected in SODs, dividing this family into Cu/Zn, Ni, Mn and Fe-SODs. Its use was limited to non-drug applications in humans (include: cosmetic, food, agriculture, and chemical industries) and drug applications in animals. This paper is a review of the recent literatures on sources of marine SODs, the need for SOD and different applications in industry, covering the last decades. The most recent paper, patents and reviews on characterization and application are reviewed., (Copyright © 2015 Elsevier B.V. All rights reserved.)

Background: The fiddler crab Ucasindensis (Alcock, 1900) (Crustacea: Brachyura: Ocypodidae) is distributed in the northern coasts of the Arabian Sea (Pakistan, Iran, Iraq, and Kuwait). Its typical habitat is on high intertidal areas with higher salinity, which might restrict its distribution, especially within the Persian Gulf. The purpose of the present phylogeographicstudy is to understand whether the Strait of Hormuz acts as a barrier to the gene flow of this species., Results: The genetic analyses of the mitochondrial 16S rRNA, cytochrome oxidase subunit I (COI), and control region (CR) of specimens from various localities showed that there was no genetic differentiation between the populations inside and outside of the Persian Gulf., Conclusions: We conclude that the narrow Strait of Hormuz does not form a barrier for the larval dispersal in this species. Its restricted distribution in the northern Arabian Sea may instead be associated with its preference for higher salinity sediments present in the coasts of thisregion.

High-tech businesses are the driving force behind global knowledge-based economies. Academic institutions have positioned themselves to serve the high-tech industry through consulting, licensing, and university spinoffs. The awareness of commercialization strategies and building an entrepreneurial culture can help academics to efficiently transfer their inventions to the market to achieve the maximum value. Here, the concept of high-tech entrepreneurship is discussed from lab to market in technology-intensive sectors such as nanotechnology, photonics, and biotechnology, specifically in the context of lab-on-a-chip devices. This article provides strategies for choosing a commercialization approach, financing a startup, marketing a product, and planning an exit. Common reasons for startup company failures are discussed and guidelines to overcome these challenges are suggested. The discussion is supplemented with case studies of successful and failed companies. Identifying a market need, assembling a motivated management team, managing resources, and obtaining experienced mentors lead to a successful exit.

A new species of freshwater crab, Sodhiana iranica n. sp. (Decapoda: Brachyura: Gecarcinucidae), is described from Bastak, Iran. This is fourth species of Sodhiana Yeo & Ng, 2012, described, and is the second one known from Iran. It can be distinguished from congeners by the form of its carapace and the structure of the male first gonopod.

Metallothionein (MT) concentrations in gill and liver tissues of Oreochromis mossambicus were determined to assess biological response of fish to levels of some metals. Metal concentrations in gill and liver tissues of O. mossambicus ranged from 0.6 to 2.6 for Cd, 16 to 52 for Zn, 0.5 to 17 for Cu and 2 to 67 for T-Hg (all in μg/g wet weight, except for T-Hg in ng/g wet weight). Accumulation of Cd, Zn, Cu and Hg (μg/g wet weight) in the liver and gills of O. mossambicus were in the order of liver > gills. The concentrations of Cd, Zn, Cu and Hg in fish tissues were correlated with MT content. The increases in MT levels from the reference area Puchong Tengah compared to the polluted area Kampung Seri Kenangan were 3.4- and 3.8-fold for gills and livers, respectively. The results indicate that MT concentrations were tissue-specific, with the highest levels in the liver. Therefore, the liver can act as a tissue indicator in O. mossambicus in the study area.

A novel luciferase from Benthosema pterotum, collected from Port of Jask, close to Persian Gulf, was purified for the first time, using Q-Sepharose anion exchange chromatography. The molecular mass of the novel enzyme, measured by SDS-PAGE technique, was about 27 kDa and its Km value is 0.4 μM; both values are similar to those of other coelenterazine luciferases. B. pterotum (BP) luciferase showed maximum intensity of emitted light at 40°C, in 20mM Tris buffer, pH 9 and 20 mM magnesium concentration. Experimental measurements indicated that BP luciferase is a relatively thermostable enzyme; furthermore it shows a high residual activity at extreme pH values. Its biological activity is strongly inhibited by 1 mM Cu(2+), Zn(2+) and Ni(2+), while calcium and mainly magnesium ions strongly increase BP luciferase activity. The B. pterotum luciferase generated blue light with a maximum emission wavelength at 475 nm and showed some similarity with other luciferases, while other parameters appeared quite different, in this way, confirming that a novel protein has been purified., (Copyright © 2013 Elsevier B.V. All rights reserved.)