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Objective: To study the wound degree and ovarian function of ultrasound interventional and laparoscopic surgeries for ovarian cyst pediculotorsion. Methods: Patients with ovarian cyst pediculotorsion admitted to Affiliated Hospital of Hebei University from May 2012 to August 2015 were selected. Patients underwent emergency ultrasound interventional operation were allocated into ultrasound intervention group and patients underwent laparoscopic surgery were allocated into laparoscopic group. The patients' conditions of two groups in perioperative period were compared. Before and after operation, the serum contents of total bilirubin, albumin, creatinine, blood urea nitrogen, tumor necrosis factor-alpha (TNF-a), interleukin-1b (IL-1b) and IL-6 were measured respectively. Serum contents of follicle-stimulating hormone, luteinizing hormone and estradiol were measured at 3 months, 6 months and 12 months after operation. Results: The operation time of ultrasound intervention group was shorter (P < 0.05) and the medical fees of it was lower than laparoscopic group (P < 0.05), while the serum contents of total bilirubin, albumin, creatinine and blood urea nitrogen during the perioperative period of two groups had no obvious changes and differences (P > 0.05). There was no significant difference in the recurrence rate after operation (P > 0.05). There was no significant difference in the serum contents of TNF-a, IL-1b, and IL-6 before and after operation in ultrasound intervention group (P > 0.05). While in laparoscopic group, serum contents of TNF-a, IL-1b and IL-6 after operation were significantly higher than those before operation (P < 0.05). And the serum contents of TNF-a, IL-1b and IL-6 after operation in ultrasound intervention group were significantly lower than those in laparoscopic group before operation (P < 0.05). The serum contents of follicle-stimulating hormone, luteinizing hormone and estradiol between two groups were not significantly different at 3 months, 6 months and 12 months after operation (P > 0.05). Conclusions: Ultrasound interventional surgery has quite the same curative effect as laparoscopic surgery in ovarian cyst pedicle pediculotorsion, which also can reduce the surgical trauma, shorten the operation time and lower the operation cost.

Purpose The purpose of this study is to identify a prospective association between CA125 and tumorigenic ovarian cancer cells, using the new method of orthotopic transplantation (1). Method After making the surgical ovarian cancer specimen into cell suspension, we separated the tumorigenic cells from the nontumorigenic cancer cells based on cell surface marker (cancer antigen CA125 and lineage markers) expression. We developed a SCID mice model in which the CA125+/ lineage- and CA125-/ lineage- cells were injected into ovarian parenchyma by use of a microinjector. As a measure of effectiveness of tumor-forming, tumor weight, abdominal distension, ascites volume and activity, subcutaneous fat were determined or observed. Immunohistochemistry was done to determine tumor cell markers. Results We found that the cells of CA125+/ lineage- were able to form new tumors; whereas, an equal quantity of CA125-/lineage- cells failed to form any tumors. The new generated tumor contained additional CA125-/lineage- tumorigenic cells as well as the phenotypically diverse population of nontumorigenic cells. Quantities were judged to be significantly different P

A new technique has successfully established the non-obese diabetic/severely combined immunodeficiency (NOD/SCID) mouse model of ovarian cancer. Under 4% chloral hydrate (0.1 mL/g dose) anesthesia, female mice were inoculated with tumor-cell suspension. The expression rate of OVCAR3 to CA125 was assessed using flow cytometry. The inoculated site was hand palpated and the signs and symptoms related to tumor growth were observed with the naked eye. The allophycocyanin (APC) indirectly labeled mouse-antihuman CA125 and fluorescein isothiocyanate (FITC)-labeled anti-mouse MHC Class I molecule (H-2K(d)/H-2D(d)) were observed using a confocal laser scanning microscope. The animal model of ovarian cancer constructed using this method can more directly reflect the characteristics of cancer cells. It provides reliable experimental results and presents a technical platform for the research of ovarian cancer stem cells.