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6 results on '"Juei-Hsiung Tsai"'

1. Significance of Salivary Epidermal Growth Factor in Peptic Ulcer Diseasein Hemodialysis Patients.

Author

Jinn-Yuh Guh, Hung-Chun Chen, Lea-Yea Chuang, Chi-Yuan Yang, Rainer, Juei-Hsiung Tsai, and Yung-Hsiung Lai

Abstract

Background/Aims: Hemodialysis (HD) patients are prone to developing peptic ulcers. However, of all the risk factors associated with peptic ulcers, none have been shown to be more prevalent in HD patients than in the general population. However, salivary epidermal growth factor (EGF) may play a role in peptic ulcer diseases. Methods: Salivary EGF levels and bioactivities were assayed in 47 maintenance HD patients and 30 normal controls, and the molecular weights of EGF were assessed using high-performance liquid chromatography (HPLC). Results: Salivary EGF levels were not different between both groups of subjects (4.2 ± 0.34 vs. 5 ± 0.54 ng/mg protein, NS), and HPLC revealed that salivary EGF in both groups had similar molecular weights. However, salivary EGF bioactivity was significantly depressed in the HD patients as compared to the normal controls (0.59 ± 0.08 vs. 1.55 ± 0.15 ng/mg protein, p < 0.01). Stepwise multiple regression showed that the low salivary EGF levels were associated with female gender (p < 0.05), while low salivary EGF bioactivity was associated with HD per se (p < 0.05). In the 22 HD patients who underwent gastric endoscopy, salivary EGF bioactivity was significantly lower in those with peptic ulcers than in those without (0.38 ± 0.08 vs. 0.69 ± 0.08 ng/mg protein, p < 0.05). Conclusion: Decreased salivary EGF bioactivity may contribute to peptic ulcer disease among maintenance HD patients.Copyright © 2001 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]

Published
2001
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3. Increased renal ANP synthesis, but decreased or unchanged cardiac ANP synthesis in water-deprived and salt-restricted rats

Author

Juei-Hsiung Tsai, Ming-Chia Hsieh, Feng-Jie Lai, Shyi-Jang Shin, Tusty-Jiuan Hsieh, Jin-Der Wen, I I-Hsuan Chen, and Mian-Shin Tan

Subjects
Male, medicine.medical_specialty, hypertension, Diuresis, Peptide hormone, Kidney, Excretion, Atrial natriuretic peptide, Internal medicine, atrial natriuretic peptide, medicine, Animals, Northern blot, Rats, Wistar, vasorelaxation, Water Deprivation, Reverse Transcriptase Polymerase Chain Reaction, Chemistry, Myocardium, Sodium, Radioimmunoassay, Diet, Sodium-Restricted, volume depletion disorders, Angiotensin II, Actins, Rats, diuresis, medicine.anatomical_structure, Endocrinology, Nephrology, cardiovascular system, aldosterone inhibition, Atrial Natriuretic Factor, hormones, hormone substitutes, and hormone antagonists, circulatory and respiratory physiology
Abstract

Increased renal ANP synthesis, but decreased or unchanged cardiac ANP synthesis in water-deprived and salt-restricted rats. Background Experiments were performed to examine the effect of water deprivation and salt restriction on ANP synthesis in the kidneys and hearts of normal rats. Methods A 4-day water deprivation (WD) and 7-day salt restriction (SR; 0.01% NaCl) were performed in 12 and 14 rats, respectively. Atrial natriuretic peptide (ANP) mRNA expression in the kidney was assessed with reverse transcription-polymerase chain reaction coupled with Southern blot hybridization, while the ANP mRNA in the hearts was measured by Northern blot hybridization. ANP and angiotensin II concentrations in the extracted plasma were measured by radioimmunoassay. The molecular form of renal ANP-like protein was characterized by reverse phase—high-performance liquid chromatography (RP-HPLC). Results Renal outer and inner medullary ANP mRNA showed a respective 11-fold and ninefold increase in WD rats, and an eightfold and fivefold increase in SR rats as compared to corresponding control groups. Inversely, cardiac atrial ANP mRNA and plasma ANP were decreased in WD rats, whereas they did not change in the SR group. Plasma angiotensin II concentration increased in conjunction with the decrease of urine sodium excretion in both groups. RP-HPLC analysis revealed a 45% extraction of ANP in the WD rat kidneys, whereas only 3% ANP in the control kidneys migrated in a molecular form similar to cardiac atrial proANP. Conclusions Our results demonstrate that water deprivation and salt restriction markedly enhance renal ANP mRNA, whereas water deprivation suppresses cardiac atrial ANP mRNA and plasma ANP concentrations. The current study indicates that renal ANP and cardiac atrial ANP appear to be two distinct systems regulated by different mechanisms and possibly exhibiting different intra-renal paracrine and systemic endocrine functions.

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5. Insulin and heparin suppress superoxide production in diabetic rat glomeruli stimulated with low-density lipoprotein.

Author

Hung-Chun Chen, Jinn-Yuh Guh, Shyi-Jang Shin, Juei-Hsiung Tsai, and Yung-Hsiung Lai

Subjects
*INSULIN, *HEPARIN, *DIABETIC neuropathies, *RATS, *PHYSIOLOGY
Abstract

Evaluates the effects of insulin and heparin on superoxide production in diabetic rat glomeruli. Level of low-density lipoprotein in patients with diabetic neuropathy; Measurement of superoxide using spectrometer; Mediation of glomerular injury in diabetics with hyperlipidemia through oxygen-free radicals.

Published
2001

6. Increase renal ANP synthesis, but decreased or unchanged cardiac ANP synthesis in water-deprive and salt-restricted rats.

Author

Shyi-Jang Shin, Jin-Der Wen, I-Hsuan Chen, Feng-Jie Lai, Ming-Chia Hsieh, Tusty-Jiuan Hsieh, Mian-Shin Tan, and Juei-Hsiung Tsai

Subjects
*ATRIAL natriuretic peptides, *HEART atrium, *KIDNEYS
Abstract

Examines the effect of water deprivation and salt restriction on atrial natriuretic peptide (ANP) synthesis in the kidney and heart of rats. Increase of renal medullary ANP messenger RNA (mRNA); Reduction of cardiac atrial ANP mRNA; Findings on the plasma angiotensin II concentration.

Published
1998
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