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1. Low-viscosity matrix suspension culture enables scalable analysis of patient-derived organoids and tumoroids from the large intestine

Author

Yumiko Hirokawa, Jordan Clarke, Michelle Palmieri, Tao Tan, Dmitri Mouradov, Shan Li, Cong Lin, Fuqiang Li, Huijuan Luo, Kui Wu, Maree Faux, Chin Wee Tan, Margaret Lee, Grace Gard, Peter Gibbs, Antony W. Burgess, and Oliver M. Sieber

Subjects
Biology (General), QH301-705.5
Abstract

Given the practical limitations of solid matrix-based protocols in organoid culture, Yumiko Hirokawa et al. assess the ability of low-concentration Matrigel conditions to promote intestinal organoid growth. Their results suggest that a low-viscosity culture system can improve live cell yield compared to the existing dome method, while maintaining similar morphology, and represents a useful approach for high-throughput applications of organoids.

Published
2021
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2. Low-viscosity Matrix Suspension Culture for Human Colorectal Epithelial Organoids and Tumoroids

Author

Tao Tan, Yumiko Hirokawa, Jordan Clarke, Anuratha Sakthianandeswaren, and Oliver Sieber

Subjects
Biology (General), QH301-705.5
Abstract

Three-dimensional culture of human normal colorectal epithelium and cancer tissue as organoids and tumoroids has transformed the study of diseases of the large intestine. A widely used strategy for generating patient-derived colorectal organoids and tumoroids involves embedding cells in domes of extracellular matrix (ECM). Despite its success, dome culture is not ideal for scalable expansion, experimentation, and high-throughput screening applications. Our group has developed a protocol for growing patient-derived colorectal organoids and tumoroids in low-viscosity matrix (LVM) suspension culture. Instead of embedding colonic crypts or tumor fragments in solid ECM, these are grown suspended in medium containing only a low percentage of ECM. Compared with dome cultures, LVM suspension culture reduces the labor and cost of establishing and passaging organoids and tumoroids, enables rapid expansion, and is readily adaptable for high-throughput screening.Graphical abstract: Generation of organoids and tumoroids from human large intestine using LVM suspension culture (Created with BioRender.com).

Published
2022
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4. Scaling dynamics of the ultracold Bose gas

Author

Ashton S. Bradley, Jordan Clarke, Tyler W. Neely, and Brian P. Anderson

Subjects
Condensed Matter::Quantum Gases, Quantum Physics, Quantum Gases (cond-mat.quant-gas), Condensed Matter::Other, FOS: Physical sciences, Computational Physics (physics.comp-ph), Quantum Physics (quant-ph), Condensed Matter - Quantum Gases, Physics - Computational Physics
Abstract

The large-scale expansion dynamics of quantum gases is a central tool for ultracold gas experiments and poses a significant challenge for theory. In this work we provide an exact reformulation of the Gross-Pitaevskii equation for the ultracold Bose gas in a coordinate frame that adaptively scales with the system size during evolution, enabling simulations of long evolution times during expansion or similar large-scale manipulation. Our approach makes no hydrodynamic approximations, is not restricted to a scaling ansatz, harmonic potentials, or energy eigenstates, and can be generalized readily to non-contact interactions via the appropriate stress tensor of the quantum fluid. As applications, we simulate the expansion of the ideal gas, a cigar-shaped condensate in the Thomas-Fermi regime, and a linear superposition of counter propagating Gaussian wavepackets. We recover known scaling for the ideal gas and Thomas-Fermi regimes, and identify a linear regime of aspect-ratio preserving free expansion; analysis of the scaling dynamics equations shows that an exact, aspect-ratio invariant, free expansion does not exist for nonlinear evolution. Our treatment enables exploration of nonlinear effects in matter-wave dynamics over large scale-changing evolution., 12 pages, 3 figures, 2 appendices

Published
2021

5. Upon the Walls of the UN Camp: Situated Intersectionality, Trajectories of Belonging, and Built Environment Among Syrian Refugees in the Kurdistan Region of Iraq.

Author

Hayes, Jordan Clarke

Subjects
BUILT environment, SYRIAN refugees, INTERSECTIONALITY, PRISONER abuse, REFUGEE children, FACADES, WALLS
Abstract

Upon the walls of homes in the Arbat 'camp' community in the Kurdistan Region of Iraq (KRI), the ubiquitous UN logo lays claim to a built environment disrupted by ornamental facades, muralism, and radical iconography. Engaging photographs and qualitative interviews gathered in the Kurdish Region of Iraq in 2017 and 2018, this essay traces expressions of "belonging" within a situated intersectional framework to surface and assess the categories of social division evident in Arbat's non-Western context. [ABSTRACT FROM AUTHOR]

Published
2020
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6. Mixed Race 3.0 : Risk and Reward in the Digital Age

Author

Ulli K. Ryder, Marcia Alesan Dawkins, Herman S. Gray, Gary B. Nash, Peggy Pascoe, Jordan Clarke, Ken Tanabe, Lori L. Tharps, Andrew K. Jolivette, Rainier Spencer, Velina Hasu Houston, Lindsay A. Dawkins, Amanda Mardon, Shoshana Sarah, Mary Beltràn, Lisa Rueckert, Ulli K. Ryder, Marcia Alesan Dawkins, Herman S. Gray, Gary B. Nash, Peggy Pascoe, Jordan Clarke, Ken Tanabe, Lori L. Tharps, Andrew K. Jolivette, Rainier Spencer, Velina Hasu Houston, Lindsay A. Dawkins, Amanda Mardon, Shoshana Sarah, Mary Beltràn, and Lisa Rueckert

Abstract

Have you been asked,'what nationality are you'or'what country are you from'?Have you been puzzled when forms tell you to'select only one ethnicity'?Have you been disturbed to hear that you're the'face of a colorblind future'?If you answered ‘yes'to any of these questions, this book is for you.Mixed Race 3.0: Risk and Reward in the Digital Age is an e-book that contains 17 contributions (many with exclusive photos) from award-winning writers, researchers and artists who embody a'mixed mindset.” Audacious and razor-sharp, Mixed Race 3.0 exposes the many monochromatic portrayals of multiracial people's richness, variety and struggles in history, politics, mass-media and technology. Fans of Loving Day, Race Remixed, The Mixed Experience Podcast, Mixed Girl Problems and Critical Mixed Race Studies will be captivated, incensed and inspired by the powerful discussions of risks and rewards of being multiracial today.Beyond memoir or case study, this book offers three versions of what it means to be mixed from a variety of voices. Version 1 is'Mixed Race 1.0: A Monologue.'Or, how did multiracial identities emerge in the U.S. and what challenges did they face? Version 2 is'Mixed Race 2.0: A Dialogue.'Or, what are some core differences between how multiracials think and talk about themselves and how U.S. and global cultures think and talk about them? Version 3 is'Mixed Race 3.0: A Megalogue.'Or, where in the world is this entire thing going as technology plays more of a role? With honest storytelling and up-to-date critical inquiry, Mixed Race 3.0 plots a path not just to being mixed in the 21st century, but one open to anyone interested in simply'how to be.'The result is a poignant, intelligent, and daring journey that dissects the controversial label—multiracial—and challenges any politician, pundit or provocateur that purports to speak for or about all multiracial people. Foreword by Herman S. Gray, Professor and Chair, American Studies, UC Santa Cruz.

Published
2015

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