Search

Background: DiGeorge syndrome (DG) is a genetic disorder associated with 22q11 deletion. It involves various phenotypes, including craniofacial abnormalities, congenital heart disorders, endocrine dysfunction, cognitive deficits, and psychiatric disorders. Cases commonly involve multiple anomalies. However, little is known about the condition of the oral cavity in this disorder, although palate fissure, abnormal mandible, malocclusion, and tooth hypoplasia have been identified. We aimed to determine the odontological features of patients with 22q11.2 microdeletion, in relation to gingival health and oral hygiene. We report the systemic manifestations of nine patients and results of oral evaluation of two patients. In the oral examination, oral hygiene and gingivitis were evaluated. Case Presentation: In terms of the systemic manifestations, we found high frequencies of low weight and height at birth. In terms of the oral manifestations, both examined patients presented malocclusion, enamel hypoplasia, dental crowding, anodontia, and healthy periodontium. Conclusion: Although DG has been documented to involve periodontium disease, the patients in this study exhibited more dental manifestations such as enamel defects, misalignment between the teeth and the two dental arches, anodontia, and dental crowding. As such, a multidisciplinary approach combining dentistry and healthcare is recommended in this case. [ABSTRACT FROM AUTHOR]

Introduction: Porphyromonas gingivalis is associated with periodontitis and exhibit a wide array of virulence factors, including fimbriae which is encoded by the FimA gene representing six known genotypes. Objetive: To identify FimA genotypes of P. gingivalis in subjects from Cali-Colombia, including the co-infection with Aggregatibacter actinomycetemcomitans, Treponema denticola, and Tannerella forsythia. Methods: Subgingival samples were collected from 151 people exhibiting diverse periodontal condition. The occurrence of P. gingivalis, FimA genotypes and other bacteria was determined by PCR. Results: Porphyromonas gingivalis was positive in 85 patients. Genotype FimA II was more prevalent without reach significant differences among study groups (54.3%), FimA IV was also prevalent in gingivitis (13.0%). A high correlation (p= 0.000) was found among P. gingivalis, T. denticola, and T. forsythia co-infection. The FimA II genotype correlated with concomitant detection of T. denticola and T. forsythia. Conclusions: Porphyromonas gingivalis was high even in the healthy group at the study population. A trend toward a greater frequency of FimA II genotype in patients with moderate and severe periodontitis was determined. The FimA II genotype was also associated with increased pocket depth, greater loss of attachment level, and patients co-infected with T. denticola and T. forsythia. [ABSTRACT FROM AUTHOR]

Background: Periodontal disease has been associated with diabetes, but there is still controversy on the relationship between periodontal clinical parameters and glycemic control. The purpose of this study is to assess the relationship between blood glucose levels and clinical parameters of periodontal disease in individuals with diabetes. Methods: A total of 65 individuals with diabetes and 81 individuals without diabetes were included in the study. A full-mouth periodontal examination and preprandial fasting glycemia values were recorded for each individual. Glycosylated hemoglobin was only measured in patients with diabetes. A comparative analysis between groups (Mann-Whitney Utest) and a correlation analysis between glycemia and periodontal parameters were performed (Spearman test). Results: Patients without diabetes presented more teeth than individuals with diabetes (P<0.05). Patients with diabetes with periodontitis displayed loss of periodontal clinical attachment compared to patients without diabetes, but the highest value was observed in patients with periodontitis that reported a smoking habit. Furthermore, patients with diabetes with periodontitis presented higher glycemia and glycated hemoglobin values in contrast to patients with gingivitis. Patients with diabetes with hyperglycemia had a higher risk to develop periodontitis (odds ratio = 2.24; 95% confidence interval = 1.02 to 4.93). A positive correlation was observed between glycemia and clinical attachment loss (AL), whereas a negative correlation between glycemia and the number of teeth present was found (P<0.05). Conclusions: Tooth and periodontal AL were increased by hyperglycemia in individuals with diabetes. This study contributes additional evidence that diabetes could aggravate periodontal disease and affect the systemic health of individuals. [ABSTRACT FROM AUTHOR]

Background: Viruses from the Herpesviridae family may be implicated in the pathogenesis of periodontal disease. The aim of this investigation was to compare the subgingival frequency of human cytomegalovirus (HCMV) in subjects affected by periodontitis to periodontally healthy subjects and to assess the correlation of HCMV with periodontal clinical parameters and periodontopathic bacteria. Methods: Thirty subjects with periodontitis (20 with chronic periodontitis and 10 with aggressive periodontitis) were included in the study. A group of 22 periodontally healthy individuals served as controls. Clinical periodontal parameters of probing depth (PD) and clinical attachment level (CAL) were recorded using a computerized periodontal probe. Subgingival plaque samples were processed for viral identification by nested polymerase chain reaction and bacterial identification by culture. Clinical periodontal parameters, frequency of detection of HCMV, and microbial composition were compared between the groups using the two-tailed Student t, χ², and Mann-Whitney tests. Logistic and linear regression analyses were performed to measure the association between virus-bacterial coinfection and clinical parameters (P≤0.05). Results: HCMV detection was more prevalent (P≤0.05) in periodontally diseased subjects compared to healthy ones. Furthermore, in all groups, PD and CAL were increased in HCMV-positive sites. In the periodontitis groups, higher frequencies and levels of specific periodontopathic bacteria were detected in HCMV positive sites. Conclusions: HCMV detection in periodontal pockets was associated with higher levels of periodontopathic bacteria and increased PD and CAL at sampled sites. HCMV/bacteria coinfection may be an important factor in periodontal destruction. [ABSTRACT FROM AUTHOR]

Background: Differences in the composition of subgingival microbiota in periodontitis have been observed in different geographic locations. Some of these microbial differences could have clinical significance. This study describes clinical parameters and the composition of the subgingival microbiota in chronic periodontitis (CP) and aggressive periodontitis (AgP) patients in a Colombian population. Methods: Clinical parameters (probing depth, clinical attachment level, bleeding on probing, and plaque index) and plaque samples from 6B CP, 12 AgP, and 30 periodontally healthy subjects were analyzed. Subgingival samples were processed using culture, biochemical tests, and polymerase chain reaction for the detection of periodontal and superinfecting pathogens. The analysis of variance, χ², and Kruskal-Wallis tests were used to evaluate differences between groups for clinical parameters and microbiologic composition (P≤0.05). Results: Clinical parameters were significantly increased in CP and AgP patients compared to healthy subjects (P<0.001), but no differences were found between periodontitis groups. Porphyromonas gingivalis, Tannerella forsythensis, and Eikenella corrodens showed higher frequencies in AgP compared to CP and healthy subjects (P <0.05). Extension (localized and generalized) of the periodontal destruction had no effect on the composition of the subgingival microbiota. Gram-negative enteric rods were more frequent in AgP patients (P<0.01). Conclusions: This study demonstrated a high prevalence of P. gingivalis, T-forsythensis, and E. corrodens in AgP patients. Gram-negative enteric rods were frequent in AgP and CP patients. Differences in the composition of subgingival microbiota in periodontitis patients need to be taken into account when considering the best therapeutic approach for each individual, including the use of antibiotics. [ABSTRACT FROM AUTHOR]

Background: The microbial profile of periodontal disease varies among different human populations. This study evaluated the demographic, clinical, and microbiologic aspects of periodontitis in a multi-geographic sample in Colombia. Methods: Three hundred twenty-five patients with chronic periodontitis (CP), 158 patients with aggressive periodontitis (AgP), and 137 healthy-gingivitis controls from five regions of the country were studied. Clinical, microbial, and sociodemographic data were collected. Microbiologic identification was performed using polymerase chain reaction 16S rRNA gene on pooled subgingival samples, and the presence of Gram-negative enteric rods was evaluated by culture. Bivariate and multivariate logistic regression analyses were conducted. Results: Porphyromonas gingivalis occurred in 71.5% of individuals with periodontitis, Tannerella forsythensis occurred in 58.5%, Campylobacter rectus occurred in 57.5%, Actinobacillus actinomycetemcomitans occurred in 23.6%, and enteric rods occurred in 34.5%. P. gingivalis was more common in CP and AgP than controls. A. actinomycetemcomitans was increased in AgP compared to controls and patients with CP. T. forsythensis, C. rectus, and Eikenella corrodens had a low presence in the West Pacific and Central regions, and enteric rods were increased in the Central region (P<0.05). Other sociodemographic factors were not associated with these microorganisms. Conclusions: Geographic regions do not influence the microbiota, but the microbiota may vary by geographic region. P. gingivalis, T. forsythensis, and C. rectus are the most prevalent periodontophatic microorganisms in Colombia. A. actinomycetemcomitans was more common in AgP, and a large percentage of the population studied had enteric rods in the subgingival plaque. [ABSTRACT FROM AUTHOR]

Focuses on the used of information technology in education in Turkey through the Computer Experimental School (CES) project. Details on CES; How Turkey increased its literacy rate; Information on the General Directorate of Computer Education and Services; In-depth look at appropriate information technology.

Introduction: To identify fimA genotypes for Porphyromonas gingivalis in diabetic and non-diabetic patients to determine the most aggressive strains and their prevalence and distribution. Objective: To identify fimA genotypes for Porphyromonas gingivalis isolates in diabetic and nondiabetic patients. Methods & materials: This was a cross-sectional descriptive study that determined the frequency of 6 genotypes of FimA for P. gingivalis in 100 patients with periodontitis and systemically healthy and 100 diabetic patients with periodontitis using conventional PCR. Results & discussion: Porphyromonas gingivalis was positive in 72 of the 100 systemically healthy patients, unlike in diabetic patients that were positive for 31 of the 100 patients. The most prevalent genotype was FimA II, with no statistically significant differences found between the groups. The distribution for healthy patients was: FimA II (57%), FimA I (20.8%), FimA III (5.5%), FimA Ib (23.6%) and FimA IV (8.3%). For patients with diabetes the frequency was: FimA II (18.75%), FimA I (15.6%), FimA III (15.6%), FimA Ib (12.5%) and FimA III (6.25%). There was a high correlation among positive samples for P. gingivalis and for A. actinomycetemcomitans, T. denticola and T. forsythia. Conclusions: The prevalence of P. gingivalis is high in healthy patients and low in diabetic patients, which shows the importance of the inflammatory response in diabetic patients. The distribution of the genotypes of FimA was heterogeneous. There were no statistically significant differences found. A co-infection relationship was observed between P. gingivalis, T forsythis. and T. denticola. [ABSTRACT FROM AUTHOR]

Descriptive observational cross-sectional study to determine the AOP of the second molars (55, 65, 75, 85) and the first permanent molars (16, 26, 36 and 46) in 459 study models corresponding to six ethnic groups in Colombia, Embera indigenous of Alto Baudó (Chocó), Caucasoid mestizos of Cali (Valle del Cauca), African descent of Cali (Valle del Cauca), Misak indigenous of Silvia (Cauca), Nasa indigenous of Morales (Cauca), and indigenous of Leticia (Amazonas). There were no significant differences of AOP among the six ethnic groups except when compared to the Amazon Indians with African descent of Cali, Embera indigenous and Nasa indigenous. There was no sexual dimorphism except tooth 65 for all ethnic groups. There was bilateral symmetry except between teeth 16 and 26. The distance matrix showed that Caucasoid mestizos of Cali were grouped with microdont populations, Amazon indigenous, Embera indigenous, Misak indigenous and Nasa indigenous, and African descendants of Cali were grouped with mesodont populations. The Embera and Amazon indigenous had the highest values of OAP associated with the relative isolation and less mestizaje. Overall, there was no sexual dimorphism or bilateral asymmetry. This study coincides with the different theories about reducing the size of the teeth as evolutionary characteristic of hominids. [ABSTRACT FROM AUTHOR]

Objective: untreated periodontal disease seems to cause low grade systemic inflammation and blood lipid alteration leading to increased cardiovascular disease risk. To start testing this hypothesis in colombian patients, a multicentre study was conducted including the three main state capitals: bogota, medellin and cali., Methods: in this study 192 (28.4%) advanced and 256 (37.8%) moderate periodontitis patients were investigated for socio-demographic variables, city of precedence, periodontal parameters, smoking, red complex periodontopathic bacteria, serum antibodies against porphyromonas gingivalis and aggregatibacter actinomycetemcomitans and blood lipids including total cholesterol, hdl, ldl and triglycerides (tg). Those parameters were compared to 229 (33.8%) controls having periodontal health or gingivitis., Results: advanced periodontitis had worst periodontal indexes, than moderate periodontitis and controls. Interestingly, higher hdl and tg levels were present in periodontitis. Bmi <30 and smoking were associated with increased hdl, hdl-35, ldl and tg, while glycemia >100 mg/dl associated with hdl, hdl-35 and tg. Tannerella forsythia showed a significant association with hdl-35 in bivariate analysis and serum igg1 against p. Gingivalis associated with hdl-35 and serum igg1 against t. Forsythia associated with tg and serum igg2 against a. Actinomycetemcomitans correlated with levels of hdl y hdl-35. In logistic regression the periodontitis patients from cali presented reduced hdl levels as compared to bogota and medellin patients. Presence of igg1 antibodies against p. Gingivalis and a. Actinomycetemcomitans correlated with reduced hdl levels., Conclusion: this study confirmed that untreated periodontitis generates alteration in serum lipid levels and systemic bacterial exposure against important periodontopathic bacteria could be the biological link.

Background: Periodontal disease has been associated with diabetes, but there is still controversy on the relationship between periodontal clinical parameters and glycemic control. The purpose of this study is to assess the relationship between blood glucose levels and clinical parameters of periodontal disease in individuals with diabetes., Methods: A total of 65 individuals with diabetes and 81 individuals without diabetes were included in the study. A full-mouth periodontal examination and preprandial fasting glycemia values were recorded for each individual. Glycosylated hemoglobin was only measured in patients with diabetes. A comparative analysis between groups (Mann-Whitney U test) and a correlation analysis between glycemia and periodontal parameters were performed (Spearman test)., Results: Patients without diabetes presented more teeth than individuals with diabetes (P <0.05). Patients with diabetes with periodontitis displayed loss of periodontal clinical attachment compared to patients without diabetes, but the highest value was observed in patients with periodontitis that reported a smoking habit. Furthermore, patients with diabetes with periodontitis presented higher glycemia and glycated hemoglobin values in contrast to patients with gingivitis. Patients with diabetes with hyperglycemia had a higher risk to develop periodontitis (odds ratio = 2.24; 95% confidence interval = 1.02 to 4.93). A positive correlation was observed between glycemia and clinical attachment loss (AL), whereas a negative correlation between glycemia and the number of teeth present was found (P <0.05)., Conclusions: Tooth and periodontal AL were increased by hyperglycemia in individuals with diabetes. This study contributes additional evidence that diabetes could aggravate periodontal disease and affect the systemic health of individuals.

Objective: The aim of this investigation was to establish the frequency of detection of periodontopathic bacteria and Gram-negative enteric rods in HIV-positive (HIV+) patients with periodontitis., Materials and Methods: Clinical parameters and microbiological samples were obtained from 31 HIV+ periodontitis (H+PG) patients, 32 HIV-negative (HIV-) periodontitis (H-PG) patients and 32 systemically and periodontally healthy (CG) patients. Microbial samples were analyzed using culturing techniques for periodontopathic and superinfecting microorganisms. The ANOVA test was used to assess differences in the clinical parameters, and the Chi square and two-tailed Mann Whitney tests were used to find differences in the composition of the microbiota (p < 0.05)., Results: The frequency of detection of periodontopathic bacteria was higher in HIV- periodontitis patients (p < 0.05) compared to HIV+ and healthy subjects. In contrast, HIV+ patients harbor higher levels of superinfecting microorganisms (p < 0.05). Pseudomonas aeruginosa, Enterobacter cloacae and Klebsiella pneumoniae were identified., Conclusions: The frequency of detection and levels of periodontopathic bacteria were higher in the HIV- periodontitis patients compared to HIV+ patients and healthy subjects. In contrast, levels of superinfecting bacteria were found to be elevated in HIV+ periodontitis patients. Special attention should be paid to the presence of periodontopathic and superinfecting bacteria in these patients in order to avoid further periodontal and systemic complications.