26 results on '"Jacquens, Lucile"'
Search Results
2. Physiological and developmental disturbances caused by Botryosphaeria dieback in the annual stems of grapevine.
- Author
-
Moret, Florian, Jacquens, Lucile, Larignon, Philippe, Clément, Gilles, Coppin, Cindy, Noirot, Elodie, Courty, Pierre-Emmanuel, Fontaine, Florence, Adrian, Marielle, and Trouvelot, Sophie
- Subjects
GRAPE diseases & pests ,DIEBACK ,GRAPES ,GENETIC overexpression ,GENE expression ,PLANT defenses - Abstract
Botryosphaeria dieback is a grapevine trunk disease caused by fungi of the Botryosphaeriaceae family, which attacks more specifically the woody tissues. The infection leads to different symptoms including a severe form with a leaf drop as well as premature plant death. Botryosphaeria dieback causes major economic losses, since no effective treatment is yet available. A better understanding is necessary to find solutions to fight this disease. In this study, our objective was to characterize the "leaf drop" form by (1) looking for the presence of pathogens in the basal internodes of stems, (2) quantifying blocked vessels by tylosis and/or gummosis, and (3) describing the impact of the disease on vine physiology (gene expression and metabolome) and development (establishment and functioning of the cambium and phellogen) at the level of xylem and phloem of basal stem internodes. Our study has shown that Botryosphaeriaceae were present in both phloem and xylem of the basal internodes of the annual stem, with xylem vessels obturated. We have also clearly demonstrated that gene expression and metabolite profiles were strongly modified in both xylem and phloem of diseased plants. Differences in stems between healthy (control, C) and diseased (D) plants were low at flowering (vines not yet symptomatic), higher at the onset of symptom expression and still present, although less marked, at full disease expression. qRT-PCR analysis showed in both phloem and xylem an overexpression of genes involved in plant defense, and a repression of genes related to meristematic activity (i.e. vascular cambium and phellogen). Metabolomic analysis showed specific fingerprints in stems of healthy and diseased plants from the onset of symptom expression, with an increase of the level of phytoalexins and mannitol, and a decrease of 1-kestose one. At the structural level, many alterations were observed in internodes, even before the onset of symptoms: a classical obstruction of xylem vessels and, for the first time, a disorganization of the secondary phloem with an obstruction of the sieve plates by callose. The disease modifies the development of both secondary phloem (liber) and phellogen. Altogether, this study combining different approaches allowed to highlight deep vine dysfunction in the internodes at the base of stems, that may explain vine decline due to Botryosphaeria dieback. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
3. Biostimulation can prime elicitor induced resistance of grapevine leaves to downy mildew
- Author
-
Jacquens, Lucile, Trouvelot, Sophie, Lemaitre-Guillier, Christelle, Krzyzaniak, Yuko, Clément, Gilles, Citerne, Sylvie, Mouille, Grégory, Moreau, Estelle, Héloir, Marie-Claire, Adrian, Marielle, Agroécologie [Dijon], Université de Bourgogne (UB)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Dijon, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), This work was supported by Region Bourgogne FrancheComté , European Union Program (Feder), BPI France andBureau Interprofessionnel des Vins de Bourgogne (FUI Iris+ and PARI Vigne Vin programs), Goëmar Laboratories., and European Project
- Subjects
phenotyping ,[SDV]Life Sciences [q-bio] ,defense elicitor ,plant immunity ,biostimulant ,grapevine - Abstract
International audience; Using plant defense elicitors to protect crops against diseases is an attractive strategy to reduce chemical pesticide use. However, development of elicitors remains limited because of variable effectiveness in the field. In contrast to fungicides that directly target pathogens, elicitors activate plant immunity, which depends on plant physiological status. Other products, the biostimulants, can improve certain functions of plants. In this study, the objective was to determine whether a biostimulant via effects on grapevine physiology could increase effectiveness of a defense elicitor. A new methodology was developed to study biostimulant activity under controlled conditions using in vitro plantlets. Both biostimulant and defense elicitor used in the study were plant extracts. When added to the culture medium, the biostimulant accelerated the beginning of plantlet growth and affected the shoot and root development. It also modified metabolomes and phytohormone contents of leaves, stems, and roots. When applied on shoots, the defense elicitor changed metabolite and phytohormone contents, but effects were different depending on whether plantlets were biostimulated or controls. Defense responses and protection against Plasmopara viticola (downy mildew agent) were induced only for plantlets previously treated with the biostimulant, Therefore, the biostimulant may act by priming the defense elicitor action. In this study, a new method to screen biostimulants active on grapevine vegetative growth was used to demonstrate that a biostimulant can optimize the efficiency of a plant defense elicitor.
- Published
- 2022
4. Sodium arsenite-induced changes in the wood of esca-diseased grapevine at cytological and metabolomic levels.
- Author
-
Trouvelot, Sophie, Lemaitre-Guillier, Christelle, Vallet, Julie, Jacquens, Lucile, Douillet, Antonin, Harir, Mourad, Larignon, Philippe, Roullier-Gall, Chloé, Schmitt-Kopplin, Philippe, Adrian, Marielle, and Fontaine, Florence
- Subjects
WOOD ,SODIUM arsenite ,METABOLOMICS ,METABOLOMIC fingerprinting ,LEAF physiology ,GRAPE diseases & pests ,GRAPES - Abstract
In the past, most grapevine trunk diseases (GTDs) have been controlled by treatments with sodium arsenite. For obvious reasons, sodium arsenite was banned in vineyards, and consequently, the management of GTDs is difficult due to the lack of methods with similar effectiveness. Sodium arsenite is known to have a fungicide effect and to affect the leaf physiology, but its effect on the woody tissues where the GTD pathogens are present is still poorly understood. This study thus focuses on the effect of sodium arsenite in woody tissues, particularly in the interaction area between asymptomatic wood and necrotic wood resulting from the GTD pathogens’ activities. Metabolomics was used to obtain a metabolite fingerprint of sodium arsenite treatment and microscopy to visualize its effects at the histo-cytological level. The main results are that sodium arsenite impacts both metabolome and structural barriers in plant wood. We reported a stimulator effect on plant secondary metabolites in the wood, which add to its fungicide effect. Moreover, the pattern of some phytotoxins is affected, suggesting the possible effect of sodium arsenite in the pathogen metabolism and/or plant detoxification process. This study brings new elements to understanding the mode of action of sodium arsenite, which is useful in developing sustainable and eco-friendly strategies to better manage GTDs. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
5. Influence of the FanLeaf Virus on the grapevine holobiont : a study from the soil to the leaves
- Author
-
Courty, Pierre-Emmanuel, Auclair, Anthony, Durney, Célien, Jacquens, Lucile, Wipf, Daniel, Mondy, Samuel, Trouvelot, Sophie, and EL Mjiyad, Noureddine
- Subjects
[SDV] Life Sciences [q-bio] - Abstract
The grapevine FanLeaf (FL) corresponds to an infectious decline (nepovirus) which appears in patches within the plots. This disease leads to a progressive degeneration of grapevine plants which ultimately leads to the death. At present, about 60% of the French vineyard would be affected, of which 30% in an important way. Sixteen nepoviruses are responsible for grapevine degeneration or decline. Nevertheless, the Grapevine FanLeaf Virus (GFLV) is the main responsible agent. It is specifically transmitted from plant to plant by a soil-borne ectoparasitic nematode, Xiphinema index. The nematodes, by successively biting two neighboring plants whose roots are close, inoculate the virus from the diseased grapevine plant to the healthy one. In spring ,new growth is languishing in infected plants. It is frequent to observe on branches a flattening (fasciation) or a shortening of the internodes, a "zigzag" growth, a division of the branch in "witches' broom". The repercussions on leaves are expressed by venation disorders, deformations and reticulated or diffuse variegations of the foliage.In a vineyard plot located in Champaign (France), rhizospheric soil, fine roots, rootstock, internodes and leaves were sampled on many symptomanic and asymptomatic grapevine plants. Meta-genomics and -transcriptomics approaches were developed to follow (i) the species distribution, (ii) the genes and (iii) the expressed genes of fungi, bacteria and virus in a continuum from the soil to the leaves. In this context, our results reveal major modifications of the grapevine holobiont during the decline Our data suggest different communities of microbes and virus among plant tissues and between symptomatic and asymptomatic plants. In addition, expressed genes of virus as well as microbes are different between symptomatic and asymtomatic plants. For the time, our results bring some informations about the influence of the microbiota and virome in the grapevine holobiont disease.
- Published
- 2022
6. Low responsiveness of grapevine flowers and berries at fruit set to UV-C irradiation
- Author
-
Petit, Anne-Noëlle, Baillieul, Fabienne, Vaillant-Gaveau, Nathalie, Jacquens, Lucile, Conreux, Alexandra, Jeandet, Philippe, Clément, Christophe, and Fontaine, Florence
- Published
- 2009
- Full Text
- View/download PDF
7. A new tool for assessment of biostimulants effects on grapevine
- Author
-
Trouvelot, Sophie, Adrian, Marielle, Heloir, Marie-Claire, Jacquens, Lucile, Krzyzaniak, Yuko, Laybros, Anthony, Cointault, Frédéric, Simon, Jean-Claude, Moreau, Estelle, Consortium, And Other Members of The Fui Iris+, Fui Iris+ Consortium, ., Agroécologie [Dijon], Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Botanique et Modélisation de l'Architecture des Plantes et des Végétations (UMR AMAP), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD [France-Sud]), Arysta LifeScience, Université Bourgogne Franche-Comté [COMUE] (UBFC), and ProdInra, Migration
- Subjects
[SDV] Life Sciences [q-bio] ,[SDE] Environmental Sciences ,biostimulants ,phenotyping ,[SDV]Life Sciences [q-bio] ,[SDE]Environmental Sciences ,food and beverages ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,[SDV.BV] Life Sciences [q-bio]/Vegetal Biology ,grapevine - Abstract
National audience; Despite an increasing interest for the use of biostimulants (BS) in agriculture, methods allowing a precise description of their effects on plants remain rather limited. In the IRIS+ FUI project, two major and highly different worldwide crops, wheat (annual, monocotyledon) and grapevine (perennial, broadleaf) were chosen to deepen our knowledge of such compounds and explore their potential additional interest. One objective was to develop a tool adapted to the screening and study of the impact of a series of BS on the development and physiology of these crops in controlled conditions. We managed to develop such a tool adapted to grapevine herbaceous cuttings. It allows a quite rapid and non-destructive phenotyping of BS effects on the aerial and root parts of plantlets over time. At the end of the experiment, plant material can be sampled for biomass measurements (fresh and dry weight) and analysis of gene expression or metabolites. This tool was validated with some commercial BS prior being used to study new putative ones. Using this tool, we have identified a new BS provided by Arysta-Goëmar. The positive effects of this BS was dose dependent. We have observed that it transiently accelerates the shoot development and increases the number of roots, and also shoot and root biomass. Moreover, analysis of the root and shoot metabolomes highlighted clear fingerprints associated to BS effects. This tool will be useful to screen new BS potentially active in grapevine. Moreover, it will be useful to study and compare the effects of diverse BS on grapevine development, metabolism and physiology ; and to decipher their mode of action. In this context, work is still in progress to further develop this tool to make it suitable to the highlighting of diverse effects related to BS and other crops, such as wheat.
- Published
- 2018
8. Clone-dependent expression of grapevine esca-disease. International congress on grapevine and wine research
- Author
-
F, MORET, C, LEMAITRE-GUILLEC, G, CLMEENT, C, COELHO, J, NEGREL, R, GOUGEON, G, MORVAN, G, MOUILLE, M, ADRIAN, Moret, Florian, Lemaître-Guillier, Christelle, Grosjean, Claire, Clément, Gilles, Coelho, Christian, Negrel, Jonathan, Jacquens, Lucile, Morvan, Guillaume, MOUILLE, Grégory, Trouvelot, Sophie, Fontaine, Florence, Adrian, Marielle, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
[SDV]Life Sciences [q-bio] ,food and beverages - Abstract
International audience; The occurrence of grapevine trunk diseases (GTDs) increases gradually within vineyards, leading to important economic losses. The worldwide cost of dead grapevines replacement is estimated over 1.5 billion dollars per year (Hofstetter et al., 2012). In France, close to 13% of the vineyard is affected (Bruez et al., 2013). Several factors could affect grapevine susceptibility to GTDs, mainly climate, vine age, soil fertilization, and also rootstock and variety (Surico et al., 2006). Presently, no cultivar tolerant to GTDs could be identified and the level of disease expression for a cultivar can vary with region and from year to year (Surico et al., 2006). In this context, our objective was to assess if the expression of esca disease, one of the most widespread GTDs, was clone-dependent.
- Published
- 2018
9. Metabolomic and cytological approaches to better understand grapevine trunk diseases disorders
- Author
-
Trouvelot, Sophie, Guillier, Christelle, Jacquens, Lucile, Douillet, Antonin, Marchat, Samuel, Grosjean, Claire, Roullier--Gall, Chloe, Schmitt--Kopplin, Philippe, Larignon, Philippe, Fontaine, Florence, Adrian, Marielle, and ROBERRINI, ISABELLE
- Subjects
[SDV] Life Sciences [q-bio] - Abstract
Grapevine trunk diseases (GTDs) are devastating diseases involving xylem-inhabiting fungi. Among them, Esca and Botryosphaeria dieback affect vineyards in major worldwide grape-producing areas. The causal fungi alter the woody issues and could produce toxins that disturb the plant physiology, leading to chronic or apolectic leaf symptoms. The unique effective fungicide against these diseases, sodium arsenite, is now prohibited because of toxicity. As GTDs are complex and still partly unknown, the identification of protection strategies remain a deadlock. Global and targeted approaches were therefore conducted to get new information on the impact of the diseases and sodium arsenite treatment on grapevine metabolism, development and physiology.
- Published
- 2017
10. Sodium arsenite application on plants expressing Grapevine Trunk Diseases-foliar symptoms: impact on grapevine physiology
- Author
-
Vallet, J., Robert-Siegwald, Guillaume, Guillier, Christelle, Godard, M.L., Bruez, Emilie, Adrian, Marielle, Trouvelot, Sophie, Jacquens, Lucile, Songy, Aurélie, Clément, C., Rey, Patrice, Tarnus, C., Bertsch, Christophe, Larignon, Philippe, Lebrun, Marc-Henri, and Fontaine, F.
- Subjects
arsénite de sodium ,vitis vinifera ,vigne ,maladie du bois de la vigne - Published
- 2017
11. OMICS approaches for the characterization of elicitor-induced resistance of grapevine leaves against downy mildew
- Author
-
Guillier, C, Heloir, Marie-Claire, Marianna, L, Rouillier-Gall, C, Jacquens, Lucile, Gougeon, R-D, Trouvelot, Sophie, Poinssot, Benoit, Daire, Xavier, Schmitt-Kopplin, Philippe, Adrian, Marielle, and EL Mjiyad, Noureddine
- Subjects
[SDV] Life Sciences [q-bio] - Published
- 2016
12. Cyclic lipopeptides from Bacillus subtilis activate distinct patterns of defence responses in grapevine
- Author
-
Farace, Giovanni, Fernandez, Olivier, Jacquens, Lucile, Coutte, François, Krier, François, Jacques, Philippe, Clément, Christophe, Barka, Essaid Ait, Jacquard, Cédric, Dorey, Stéphan, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
[SDV]Life Sciences [q-bio] ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
- Published
- 2015
13. Surfactin and fengycin contribute to the protection of a Bacillus subtilis strain against grape downy mildew by both direct effect and defence stimulation.
- Author
-
Li, Yan, Héloir, Marie‐Claire, Zhang, Xun, Geissler, Mareen, Trouvelot, Sophie, Jacquens, Lucile, Henkel, Marius, Su, Xin, Fang, Xuewen, Wang, Qi, and Adrian, Marielle
- Subjects
DOWNY mildew diseases ,BACILLUS subtilis ,SURFACTIN ,GRAPES ,PHYTOPATHOGENIC microorganisms ,BIOLOGICAL pest control agents - Abstract
Summary: Bacillus subtilis GLB191 (hereafter GLB191) is an efficient biological control agent against the biotrophic oomycete Plasmopara viticola, the causal agent of grapevine downy mildew. In this study, we show that GLB191 supernatant is also highly active against downy mildew and that the activity results from both direct effect against the pathogen and stimulation of the plant defences (induction of defence gene expression and callose production). High‐performance thin‐layer chromatography analysis revealed the presence of the cyclic lipopeptides fengycin and surfactin in the supernatant. Mutants affected in the production of fengycin and/or surfactin were thus obtained and allowed us to show that both surfactin and fengycin contribute to the double activity of GLB191 supernatant against downy mildew. Altogether, this study suggests that GLB191 supernatant could be used as a new biocontrol product against grapevine downy mildew. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
14. Clone-Dependent Expression of Esca Disease Revealed by Leaf Metabolite Analysis.
- Author
-
Moret, Florian, Lemaître-Guillier, Christelle, Grosjean, Claire, Clément, Gilles, Coelho, Christian, Negrel, Jonathan, Jacquens, Lucile, Morvan, Guillaume, Mouille, Grégory, Trouvelot, Sophie, Fontaine, Florence, and Adrian, Marielle
- Subjects
ESCA (Grape disease) ,FUNGAL diseases of grapes - Abstract
Grapevine trutk diseases, especially Esca, are of major concern since they gradually alter vineyards worldwide and cause heavy economic losses. The expression of Esca disease symptoms depends on several factors, including the grapevine cultivar. In this context, a possible clone-dependent expression of the Esca disease was studied. Two clones of 'Chardonnay' grown in the same plot were compared according to their developmental and physiological traits, metabolome, and foliar symptom expression. Analysis of their leaf metabolome highlighted differences related to symptom expression. Interestingly, the content of a few specific metabolites exhibited opposite variations in leaves of symptomatic shoots of clones 76 and 95. Altogether this study showed a clone-dependent expression of Esca disease in 'Chardonnay' and the relevance of GC-MS and 3D fluorescence methods to analyze the impact of the disease on the leaf metabolome. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
15. Molecular evolution within and between populations of Arabidopsis thaliana: the FRIGIDA gene
- Author
-
Le Corre, Valérie, Jacquens, Lucile, Roux, Fabrice, and Jacquens, Lucile
- Subjects
[SDV.GEN.GPL] Life Sciences [q-bio]/Genetics/Plants genetics ,[SDV.GEN.GPO] Life Sciences [q-bio]/Genetics/Populations and Evolution [q-bio.PE] - Published
- 2003
16. A Plant Extract Acts Both as a Resistance Inducer and an Oomycide Against Grapevine Downy Mildew.
- Author
-
Krzyzaniak, Yuko, Trouvelot, Sophie, Negrel, Jonathan, Cluzet, Stéphanie, Valls, Josep, Richard, Tristan, Bougaud, Ambrine, Jacquens, Lucile, Klinguer, Agnès, Chiltz, Annick, Adrian, Marielle, and Héloir, Marie-Claire
- Subjects
PLANT extracts ,DISEASE resistance of plants ,GRAPE powdery mildew disease - Abstract
Protecting vineyards from cryptogamic diseases such as downy mildew, caused by Plasmopara viticola, generally requires a massive use of phytochemicals. However, the issues on unintentional secondary effects on environment and human health, and the occurrence of P. viticola resistant strains, are leading to the development of alternative strategies, such as the use of biocontrol products. In this paper, we evidenced the ability of a plant extract to protect grapevine from P. viticola. Further experiments carried out both on cell suspensions and on plants revealed that plant extract activates typical defense-related responses such as the production of H
2 O2 , the up-regulation of genes encoding pathogenesis-related proteins and stilbene synthase, as well as the accumulation of resveratrol or its derivative piceid. We also brought to light a strong direct effect of PE on the release and motility of P. viticola zoospores. Furthermore, we found out that PE application left dried residues on leaf surface, impairing zoospores to reach stomata. Altogether, our results highlight the different modes of action of a new biocontrol product able to protect grapevine against downy mildew. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
- View/download PDF
17. Distinct regulation in inflorescence carbohydrate metabolism according to grapevine cultivars during floral development.
- Author
-
Sawicki, Mélodie, Jacquens, Lucile, Baillieul, Fabienne, Clément, Christophe, Vaillant‐Gaveau, Nathalie, and Jacquard, Cédric
- Subjects
- *
CARBOHYDRATE metabolism , *PLANT reproduction , *PHOTOSYNTHESIS , *VITIS vinifera , *PINOT noir - Abstract
Carbohydrate metabolism is important in plant sexual reproduction because sugar contents are determining factors for both flower initiation and floral organ development. In woody plants, flowering represents the most energy-consuming step crucial to reproductive success. Nevertheless, in these species, the photosynthesis performed by flowers supplies the carbon required for reproduction. In grapevine (Vitis vinifera), the inflorescence has a specific status because this organ imports carbohydrates at the same time as it exports photoassimilates. In this study, fluctuations in carbohydrate metabolism were monitored by analyzing gas exchanges, photosynthetic electron transport capacity, carbohydrate contents and some activities of carbohydrate metabolism enzymes, in the inflorescences of Pinot noir and Gewurztraminer, two cultivars with a different sensitivity to coulure phenomenon. Our results showed that photosynthetic activity and carbohydrate metabolism are clearly different and differently regulated during the floral development in the two cultivars. Indeed, the regulation of the linear electron flow and the cyclic electron flow is not similar. Moreover, the regulation of PSII activity, with a higher Y(NPQ)/Y(NO) ratio in Gewurztraminer, can be correlated with the higher protection of the photosynthetic chain and consequently with the higher yield under optimal conditions of this cultivar. At least, our results showed a higher photosynthetic activity and a better protection of PSI in Pinot noir during the floral development. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
18. Inflorescences vs leaves: a distinct modulation of carbon metabolism process during Botrytis infection.
- Author
-
Vatsa‐Portugal, Parul, Walker, Anne‐Sophie, Jacquens, Lucile, Clément, Christophe, Barka, Essaid Ait, and Vaillant‐Gaveau, Nathalie
- Subjects
INFLORESCENCES ,LEAF physiology ,BOTRYTIS ,CARBON metabolism ,PHOTOSYSTEMS - Abstract
Plant growth and survival depends critically on photo assimilates. Pathogen infection leads to changes in carbohydrate metabolism of plants. In this study, we monitored changes in the carbohydrate metabolism in the grapevine inflorescence and leaves using Botrytis cinerea and Botrytis pseudo cinerea. Fluctuations in gas exchange were correlated with variations in chlorophyll a fluorescence. During infection, the inflorescences showed an increase in net photosynthesis (Pn) with a stomatal limitation. In leaves, photosynthesis decreased, with a non-stomatal limitation. A decrease in the effective photosystem II (PSII) quantum yield (ΦPSII) was accompanied by an increase in photochemical quenching (qP) and non-photochemical quenching (qN). The enhancement of qP and ΦPSII could explain the observed increase in Pn. In leaves, the significant decline in ΦPSII and qP, and increase in qN suggest that energy was mostly oriented toward heat dissipation instead of CO
2 fixation. The accumulation of glucose and sucrose in inflorescences and glucose and fructose in the leaves during infection indicate that the plant's carbon metabolism is differently regulated in these two organs. While a strong accumulation of starch was observed at 24 and 48 hours post-inoculation (hpi) with both species of Botrytis in the inflorescences, a significant decrease with B. cinerea at 24 hpi and a significant increase with B. pseudo cinerea at 48 hpi were observed in the leaves. On the basis of these results, it can be said that during pathogen attack, the metabolism of grapevine inflorescence and leaf is modified suggesting distinct mechanisms modifying gas exchange, PSII activity and sugar contents in these two organs. [ABSTRACT FROM AUTHOR]- Published
- 2015
- Full Text
- View/download PDF
19. Expression Analysis in Grapevine by In Situ Hybridization and Immunohistochemistry.
- Author
-
Colas, Steven, Jacquens, Lucile, Manteau, Sébastien, Devy, Jérome, Conéjéro, Geneviève, Clément, Christophe, Baillieul, Fabienne, Mazeyrat-Gourbeyre, Florence, and Monti-Dedieu, Laurence
- Abstract
In situ hybridization (ISH) associated to immunohistochemistry has become a powerful tool for the examination of gene expression. Application of these techniques in grapevine tissues is limited mainly because of technical difficulties with this plant material. Here we present detailed protocols for ISH and immunohistochemistry, recommended controls and troubleshooting, along with examples of several applications to grapevine tissues. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
20. Expression and In Situ Localization of Two Major PR Proteins of Grapevine Berries during Development and after UV-C Exposition.
- Author
-
Colas, Steven, Afoufa-Bastien, Damien, Jacquens, Lucile, Clément, Christophe, Baillieul, Fabienne, Mazeyrat-Gourbeyre, Florence, Monti-Dedieu, Laurence, and Gasset, Maria
- Subjects
VITIS vinifera ,PLANT development ,PLANT defenses ,GENE expression in plants ,IN situ hybridization ,GENETIC regulation in plants - Abstract
In grapevine Vitis vinifera L. cv Pinot noir, the Pathogenesis-Related (PR) proteins CHI4D and TL3 are among the most abundant extractable PR proteins of ripe berries and accumulate during berry ripening from véraison until full maturation. Evidence was supplied in favor of the involvement of these two protein families in plant defense mechanisms and plant development. In order to better understand CHI4D and TL3 function in grapevine, we analyzed their temporal and spatial pattern of expression during maturation and after an abiotic stress (UV-C) by in situ hybridization (ISH) and immunohistolocalization. In ripening berries, CHI4D and TL3 genes were mainly expressed in the exocarp and around vascular bundles of the mesocarp. In UV-C exposed berries, CHI4D and TL3 gene expression was strongly induced before ve´raison. Corresponding proteins localized in the exocarp and, to a lesser extent, around vascular bundles of the mesocarp. The spatial and temporal accumulation of the two PR proteins during berry maturation and after an abiotic stress is discussed in relation to their putative roles in plant defense. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
21. Leaf stripe form of esca induces alteration of photosynthesis and defence reactions in presymptomatic leaves.
- Author
-
Magnin-Robert, Maryline, Letousey, Patricia, Spagnolo, Alessandro, Rabenoelina, Fanja, Jacquens, Lucile, Mercier, Laurence, Clément, Christophe, and Fontaine, Florence
- Subjects
PHOTOSYNTHESIS ,PLANT diseases ,GRAPES ,CHLOROPHYLL ,FLUORESCENCE - Abstract
Esca is a destructive disease in grapevines (Vitis vinifera L.) caused by at least three fungi and characterised by two different external symptoms, the apoplectic and leaf stripe form. This latter form can be discerned as soon as symptoms become visible, but the preceding discrete signs during incubation are poorly or not understood. To further understand the development of the leaf stripe form, the period preceding and following the appearance of symptoms was investigated by studying physiological and molecular markers associated with photosynthetic mechanisms and stress response. No perturbation of any targeted metabolism was observed in asymptomatic leaves of asymptomatic canes from vines showing the leaf stripe form of esca. Conversely, drastic alterations of photosynthesis functions were registered in presymptomatic leaves, as revealed by the decrease of gas exchange and chlorophyll fluorescence, and the repression of photosynthesis-related genes. These alterations were amplified during symptom development. Expression of defence-related genes was affected and detected early in presymptomatic leaves and amplified during symptom expression. Our results suggest that grapevines may react precociously by reducing photosynthesis and triggering defence mechanisms in response to the leaf stripe form of esca. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
22. VOCs Are Relevant Biomarkers of Elicitor-Induced Defences in Grapevine.
- Author
-
Lemaitre-Guillier, Christelle, Dufresne, Christelle, Chartier, Agnès, Cluzet, Stéphanie, Valls, Josep, Jacquens, Lucile, Douillet, Antonin, Aveline, Nicolas, Adrian, Marielle, and Daire, Xavier
- Subjects
GRAPES ,VOLATILE organic compounds ,DOWNY mildew diseases ,BIOMARKERS ,MYCOSES - Abstract
Grapevine is susceptible to fungal diseases generally controlled by numerous chemical fungicides. Elicitors of plant defence are a way of reducing the use of these chemicals, but still provide inconsistent efficiency. Easy-to-analyse markers of grapevine responses to elicitors are needed to determine the best conditions for their efficiency and position them in protection strategies. We previously reported that the elicitor sulphated laminarin induced the emission of volatile organic compounds (VOCs) by grapevine leaves. The present study was conducted to characterise and compare VOC emissions in response to other elicitors. Bastid
® was first used to test the conditions of VOC collection and analysis. Using SBSE-GC-MS, we detected several VOCs, including the sesquiterpene α-farnesene, in a time-dependent manner. This was correlated with the induction of farnesene synthase gene expression, in parallel with stilbene synthesis (another defence response), and associated to resistance against downy mildew. The other elicitors (Redeli® , Romeo® , Bion® , chitosan, and an oligogalacturonide) induced VOC emission, but with qualitative and quantitative differences. VOC emission thus constitutes a response of grapevine to elicitors of various chemical structures. Therefore, VOC analysis is relevant for studying the impact of environmental factors on grapevine defence responses and optimising the performance of elicitors in vineyards. [ABSTRACT FROM AUTHOR]- Published
- 2021
- Full Text
- View/download PDF
23. Assessment of the impact of PS3-induced resistance to downy mildew on grapevine physiology.
- Author
-
Héloir, Marie-Claire, Kim Khiook, Ian Li, Lemaître-Guillier, Christelle, Clément, Gilles, Jacquens, Lucile, Bernaud, Eric, Trouvelot, Sophie, and Adrian, Marielle
- Subjects
- *
DOWNY mildew diseases , *DISEASE resistance of plants , *GRAPEVINE irrigation , *FUNGICIDE analysis , *METABOLITES - Abstract
Abstract Elicitor-induced resistance against diseases is an attractive strategy that could contribute to reduce the use of fungicides for plant protection. However, activation of defenses has an energetic cost that plants have to fuel by a mobilization of their primary metabolism with possible adverse effect on their physiology. In this context, this study was performed to determine whether elicitor-induced resistance of grapevine leaves against downy mildew impacted its development and metabolism. The elicitor PS3 (sulfated β-glucan laminarin) was sprayed on grapevine herbaceous cuttings grown in greenhouses once or three times, and its impact was studied on young and older grapevine leaves, prior to, and after Plasmopara viticola inoculation. PS3 did not affect grapevine development during the time course of the experiment. A metabolomic analysis, mainly focused on primary metabolites, highlighted a leaf age dependent effect of PS3 treatment. Nitrogen compounds, and sugars to a lesser extent, were impacted. The results obtained complete the current knowledge of the impact of elicitor-induced resistance on plant physiology. They will be helpful to guide further experiments required to better determine the costs and benefits of elicitor-induced resistance in plants. Graphical abstract Image 1 Highlights • PS3-induced resistance of grapevine leaves against downy mildew does not affect the plant development. • PS3-induced resistance of grapevine leaves against downy mildew affect the leaf primary metabolism. • PS3-induced resistance of grapevine leaves against downy mildew impacts nitrogen compounds, and sugars to a lesser extent. • Effect of PS3 on the leaf primary metabolism is leaf age-dependent. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
24. Alterations in Grapevine Leaf Metabolism Occur Prior to Esca Apoplexy Appearance.
- Author
-
Magnin-Robert M, Adrian M, Trouvelot S, Spagnolo A, Jacquens L, Letousey P, Rabenoelina F, Harir M, Roullier-Gall C, Clément C, Schmitt-Kopplin P, Vallat A, Abou-Mansour E, and Fontaine F
- Subjects
- Cyclotrons, Fourier Analysis, Fungi physiology, Gene Expression Profiling, Gene Expression Regulation, Plant, Genetic Association Studies, Glutathione metabolism, In Situ Hybridization, Mass Spectrometry, Metabolic Networks and Pathways, Metabolome genetics, Plant Leaves genetics, Plant Proteins genetics, Plant Proteins metabolism, RNA, Messenger genetics, RNA, Messenger metabolism, Reproducibility of Results, Vitis genetics, Plant Diseases microbiology, Plant Leaves metabolism, Plant Leaves microbiology, Vitis metabolism, Vitis microbiology
- Abstract
Esca disease is one of the major grapevine trunk diseases in Europe and the etiology is complex, since several inhabiting fungi are identified to be associated with this disease. Among the foliar symptom expressions, the apoplectic form may be distinguished and characterized by sudden dieback of shoots, leaf drop, and shriveling of grape clusters in a few days that can ultimately induce the plant death. To further understand this drastic event, we conducted transcriptomic and metabolomic analyses to characterize responses of leaves during the period preceding symptom appearance (20 and 7 days before foliar symptom expression) and at the day of apoplexy expression. Transcriptomic and metabolomic analyses provide signatures for the apoplectic leaves and most changes concerning the metabolism of carbohydrates, amino acids, and phenylpropanoids. In deciphering glutathione-S-transferase (GST), its preferential location in phloem, correlated with the upregulation of GST genes and a decrease of the glutathione level, offers further support to the putative role of glutathione during apoplexy expression.
- Published
- 2017
- Full Text
- View/download PDF
25. Cyclic lipopeptides from Bacillus subtilis activate distinct patterns of defence responses in grapevine.
- Author
-
Farace G, Fernandez O, Jacquens L, Coutte F, Krier F, Jacques P, Clément C, Barka EA, Jacquard C, and Dorey S
- Subjects
- Botrytis metabolism, Fatty Acids pharmacology, Lipopeptides pharmacology, Peptides, Cyclic pharmacology, Bacillus subtilis metabolism, Oligopeptides pharmacology, Vitis drug effects, Vitis metabolism
- Abstract
Non-self-recognition of microorganisms partly relies on the perception of microbe-associated molecular patterns (MAMPs) and leads to the activation of an innate immune response. Bacillus subtilis produces three main families of cyclic lipopeptides (LPs), namely surfactins, iturins and fengycins. Although LPs are involved in induced systemic resistance (ISR) activation, little is known about defence responses induced by these molecules and their involvement in local resistance to fungi. Here, we showed that purified surfactin, mycosubtilin (iturin family) and plipastatin (fengycin family) are perceived by grapevine plant cells. Although surfactin and mycosubtilin stimulated grapevine innate immune responses, they differentially activated early signalling pathways and defence gene expression. By contrast, plipastatin perception by grapevine cells only resulted in early signalling activation. Gene expression analysis suggested that mycosubtilin activated salicylic acid (SA) and jasmonic acid (JA) signalling pathways, whereas surfactin mainly induced an SA-regulated response. Although mycosubtilin and plipastatin displayed direct antifungal activity, only surfactin and mycosubtilin treatments resulted in a local long-lasting enhanced tolerance to the necrotrophic fungus Botrytis cinerea in grapevine leaves. Moreover, challenge with specific strains overproducing surfactin and mycosubtilin led to a slightly enhanced stimulation of the defence response compared with the LP-non-producing strain of B. subtilis. Altogether, our results provide the first comprehensive view of the involvement of LPs from B. subtilis in grapevine plant defence and local resistance against the necrotrophic pathogen Bo. cinerea. Moreover, this work is the first to highlight the ability of mycosubtilin to trigger an immune response in plants., (© 2014 BSPP AND JOHN WILEY & SONS LTD.)
- Published
- 2015
- Full Text
- View/download PDF
26. Burkholderia phytofirmans PsJN acclimates grapevine to cold by modulating carbohydrate metabolism.
- Author
-
Fernandez O, Theocharis A, Bordiec S, Feil R, Jacquens L, Clément C, Fontaine F, and Barka EA
- Subjects
- Bacterial Proteins genetics, Bacterial Proteins metabolism, Burkholderia genetics, Carbohydrates physiology, Gene Expression Regulation, Bacterial physiology, Glycolysis physiology, Photosynthesis physiology, Pigments, Biological metabolism, Starch metabolism, Adaptation, Physiological physiology, Burkholderia metabolism, Cold Temperature, Vitex microbiology, Vitex physiology
- Abstract
Low temperatures damage many temperate crops, including grapevine, which, when exposed to chilling, can be affected by symptoms ranging from reduced yield up to complete infertility. We have previously demonstrated that Burkholderia phytofirmans PsJN, a plant growth-promoting rhizobacteria (PGPR) that colonizes grapevine, is able to reduce chilling-induced damage. We hypothesized that the induced tolerance may be explained at least partly by the impact of bacteria on grapevine photosynthesis or carbohydrate metabolism during cold acclimation. To investigate this hypothesis, we monitored herein the fluctuations of photosynthesis parameters (net photosynthesis [P(n)], intercellular CO(2) concentration, stomatal conductances, ΦPSII, and total chlorophyll concentration), starch, soluble sugars (glucose, fructose, saccharose, mannose, raffinose, and maltose), and their precursors during 5 days of chilling exposure (4°C) on grapevine plantlets. Bacterization affects photosynthesis in a non-stomatal dependent pattern and reduced long-term impact of chilling on P(n). Furthermore, all studied carbohydrates known to be involved in cold stress tolerance accumulate in non-chilled bacterized plantlets, although some of them remained more concentrated in the latter after chilling exposure. Overall, our results suggest that modification of carbohydrate metabolism in bacterized grapevine plantlets may be one of the major effects by which this PGPR reduces chilling-induced damage.
- Published
- 2012
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.