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Appropriate protection and guiding are crucial during peripheral nerves repair. New generation nerve guidance conduits (NGCs) should not only provide mechanical support for the damaged nerve but also support healing processes. One of the most promising tissue regeneration applications is fibrous biomaterials since they are characterized by high porosity, flexibility, and strength. Additionally, they enable cell adhesion and proliferation. In this study, novel fibrous nanocomposites were obtained by applying the electrospinning technique, using polylactic acid (PLA) as a polymeric matrix which was further modified with metallic nanoparticles coated with conductive polymers. Such an approach resulted in the obtainment of biomaterials with a potential ability to conduct nerve impulses. The chemical structure of the obtained composites, as well as the morphology of ready products and separate nanocomponents, were investigated using Fourier-transform infrared spectroscopy (FTIR), transmission electron microscope (TEM) and scanning electron microscope (SEM) techniques. Furthermore, conductive and swelling properties in various media were determined. Finally, biomaterials were confirmed to be non-cytotoxic to L929 mouse fibroblasts and 1321N1 human glial cells. Based on the presented results, it can be concluded that nanofibrous nerve guidance conduits have all the key properties in the process of peripheral nerve regeneration and may constitute an important step in novel NGCs development. [ABSTRACT FROM AUTHOR]

This prospective study aimed to assess the feasibility of chitosan biomaterial and subcutaneous gel implantation in an ovine model, with implications for women with genital prolapse. Twenty-four ewes were divided into four groups (n = 6 per group): chitosan type B, chitosan type C, chitosan unmodified injections, and polypropylene mesh. Ovine models were chosen due to their morphological resemblance to human reproductive organs. Animals were sacrificed after 90 days for macroscopic, pathomorphological, and immunohistochemical analysis. In the chitosan type B group, IL-6 and IL-10 levels decreased after 28 days, while chitosan type C and injection groups exhibited higher IL-6 than IL-10 levels. The polypropylene group displayed the highest IL-6 and lowest IL-10 levels. Histological examination of the polypropylene group revealed no degenerative changes or inflammation, whereas chitosan injection induced local inflammation. Other groups exhibited no degenerative changes. Ewes implanted with chitosan displayed reduced inflammation compared to polypropylene-implanted ewes. Chitosan implantation facilitated vaginal tissue healing, in contrast to polypropylene mesh, which led to extrusion. While chitosan holds promise as an alternative to polypropylene mesh, further research is imperative for comprehensive evaluation. This study suggests the potential of a chitosan biomaterial in pelvic organ prolapse treatment, warranting additional investigation. [ABSTRACT FROM AUTHOR]

Tissue engineering constitutes the most promising method of severe peripheral nerve injuries treatment and is considered as an alternative to autografts. To provide appropriate conditions during recovery special biomaterials called nerve guide conduits are required. An ideal candidate for this purpose should not only be biocompatible and protect newly forming tissue but also promote the recovery process. In this article a novel, multilayered biomaterial based on polyvinylpyrrolidone, collagen and chitosan of gradient structure modified with conductive nanoparticles is presented. Products were obtained by the combination of electrospinning and electrospraying techniques. Nerve guide conduits were subjected to FT-IR analysis, morphology and elemental composition study using SEM/EDS as well as biodegradation. Furthermore, their effect on 1321N1 human cell line was investigated by long-term cell culture. Lack of cytotoxicity was confirmed by XTT assay and morphology study. Obtained results confirmed a high potential of newly developed biomaterials in the field of nerve tissue regeneration with a special focus on injured nerves recovery. [ABSTRACT FROM AUTHOR]

Rare Earth elements (REE) such as NdFeB are commonly used to produce permanent magnets. Thanks to their superior properties, these materials are highly desirable for green energy applications such as wind power generators or electric cars. Currently, REEs are critical for the ongoing development of eco-friendly solutions in different industrial branches. The emerging issue of REE depletion has led to a need for new methods to enable the life cycle elongation, resistance to wear, and external factors improvement of NdFeB magnets. This can be achieved by advanced, nanostructured coating formation of magnet surfaces to increase their functionality and protect from humidity, pressure, temperature, and other factors. The aim of the following research was to develop a new, scalable strategy for the modification of NdFeB magnets using laser-assisted technique, also known as Laser cladding. For this purpose, four different micropowders were used to modify commercial NdFeB samples. The products were investigated for their morphology, structure, chemical composition, and crystallography. Moreover, magnetic flux density was evaluated. Our results showed that laser cladding constitutes a promising strategy for REE-based permanent magnets modification and regeneration and may help to improve durability and resistance of NdFeB components. [ABSTRACT FROM AUTHOR]

The present study demonstrates a strategy for preparing porous composite fibrous materials with superior biocompatibility and antibacterial performance. The findings reveal that the incorporation of PEG into the spinning solutions significantly influences the fiber diameters, morphology, and porous area fraction. The addition of a hydrophilic homopolymer, PEG, into the Ch/PLA spinning solution enhances the hydrophilicity of the resulting materials. The hybrid fibrous materials, comprising Ch modified with PLA and PEG as a co-solvent, along with post-treatment to improve water stability, exhibit a slower rate of degradation (stable, moderate weight loss over 16 weeks) and reduced hydrophobicity (lower contact angle, reaching 21.95 ± 2.17°), rendering them promising for biomedical applications. The antibacterial activity of the membranes is evaluated against Staphylococcus aureus and Escherichia coli, with PEG-containing samples showing a twofold increase in bacterial reduction rate. In vitro cell culture studies demonstrated that PEG-containing materials promote uniform cell attachment, comparable to PEG-free nanofibers. The comprehensive evaluation of these novel materials, which exhibit improved physical, chemical, and biological properties, highlights their potential for biomedical applications in tissue engineering and regenerative medicine. [ABSTRACT FROM AUTHOR]

An increasing number of tooth replacement procedures ending with implant failure generates a great need for the delivery of novel biomedical solutions with appropriate mechanical characteristics that would mimic natural tissue and undergo biodegradation. This phenomenon constitutes a significant difficulty for scientists, since currently applied biomaterials dedicated for this purpose are based on stainless steel, Ti, and Ti and CoCr alloys. One of the most promising raw materials is magnesium, which has been proven to promote bone regeneration and accelerate the tissue healing process. Nevertheless, its high reactivity with body fluid components is associated with fast and difficult-to-control biocorrosion, which strongly limits the application of Mg implants as medical devices. The achievement of appropriate functionality, both physiochemical and biological, to enable the commercial use of Mg biomaterials is possible only after their superficial modification. Therefore, the obtainment of uniform, reproducible coatings increasing resistance to the aqueous environment of the human body combined with a nanostructured surface that enhances implant–cell behaviors is an extremely important issue. Herein, we present a successful strategy for the modification of Mg implants via the PEO process, resulting in the obtainment of biomaterials with lower corrosion rates and superior biological properties, such as the promotion of extracellular matrix formation and a positive impact on the proliferation of MG-63 cells. The implants were investigated regarding their chemical composition using the FT-IR and XRD methods, which revealed that MgO layer formation, as well as the incorporation of electrolyte components such as fluorine and silica, were responsible for the increased microhardness of the samples. An extensive study of the biomaterials' morphology confirmed that successful surface modification led to a microporous structure suitable for the attachment and proliferation of cells. The three-layer nature of the newly-formed coatings, typical for PEO modification, was confirmed via cross-section analysis. A biocorrosion and biodegradation study proved that applied modification increased their resistance to body fluids. The cell culture study performed herein confirmed that the correct adjustment of modification parameters results in a lack of cytotoxicity of the magnesium implants, cell proliferation enhancement, and improvement in extracellular matrix formation. [ABSTRACT FROM AUTHOR]

Chitosan, a natural biopolymer, is an ideal candidate to prepare biomaterials capable of preventing microbial infections due to its antibacterial properties. Electrospinning is a versatile method ideally suited to process biopolymers with minimal impact on their physicochemical properties. However, fabrication parameters and post-processing routine can affect biological activity and, therefore, must be well adjusted. In this study, nanofibrous membranes were prepared using trifluoroacetic acid and dichloromethane and evaluated for physiochemical and antimicrobial properties. The use of such biomaterials as potential antibacterial agents was extensively studied in vitro using Staphylococcus aureus and Escherichia coli as test organisms. The antibacterial assay showed inhibition of bacterial growth and eradication of the planktonic cells of both E. coli and S. aureus in the liquid medium for up to 6 hrs. The quantitative assay showed a significant reduction in bacteria cell viability by nanofibers depending on the method of fabrication. The antibacterial properties of these biomaterials can be attributed to the structural modifications provided by co-solvent formulation and application of post-treatment procedure. Consequently, the proposed antimicrobial surface modification method is a promising technique to prepare biomaterials designed to induce antimicrobial resistance via antiadhesive capability and the biocide-releasing mechanism. [ABSTRACT FROM AUTHOR]

The ear pinna is a complex tissue consisting of the dermis, cartilage, muscles, vessels, and nerves. Ear pinna healing is a model of regeneration in mammals. In some mammals, including rabbits, punch wounds in the ear pinna close spontaneously; in common-use laboratory mice, they remain for life. Agents inducing ear pinna healing are potential regenerative drugs. We tested the effects of selected bioactive agents on 2 mm ear pinna wound closure in BALB/c mice. Our previous research demonstrated that a DNA methyltransferase inhibitor, zebularine, remarkably induced ear pinna regeneration. Although experiments with two other demethylating agents, RG108 and hydralazine, were unsuccessful, a histone deacetylase inhibitor, valproic acid, was another epigenetic agent found to increase ear hole closure. In addition, we identified a pro-regenerative activity of 4-ketoretinoic acid, a retinoic acid metabolite. Attempts to counteract the regenerative effects of the demethylating agent zebularine, with folates as methyl donors, failed. Surprisingly, a high dose of methionine, another methyl donor, promoted ear hole closure. Moreover, we showed that the regenerated areas of ear pinna were supplied with nerve fibre networks and blood vessels. The ear punch model proved helpful in testing the pro-regenerative activities of small-molecule compounds and observations of peripheral nerve regeneration. [ABSTRACT FROM AUTHOR]

Skin is the first barrier against pathogens and harmful external factors. Each damage of this tissue may cause microbial infection and danger to internal organs. Burns which may be a result of the exposure to radiation, chemicals or high temperature leads to the significant disruption of skin functions. The most promising method for this tissue recovery is regenerative medicine which requires application of three-dimensional biocompatible scaffolds. The biomaterials enable skin cells proliferation and new tissue formation under in vitro conditions. They can be prepared from synthetic and natural polymers and their combination. The application of additional components such as nanoparticles may enhance their mechanical properties and have a positive impact on fibroblasts divisions and extra cellular formation. One of the most promising raw materials for scaffolds is chitosan -a chitin derivative. It may be obtained from waste biomass such as crabs, shrimps and lobsters exoskeletons. Chitosan is non-toxic, biodegradable and have antibacterial properties. The aim of the following study was to obtain novel chitosan derivatives doped with the gold nanoparticles using only natural components such as orange peels and fatty acid derivative. Proposed modification strategy resulted in the preparation of the novel, biodegradable and biocompatible material with interesting properties. The products were analysed by UV-Vis and FT-IR methods. The scaffolds were investigated over their susceptibility to enzymatic degradation. Finally, the biomaterials were verified over their cyto-compability with human dermal fibroblasts. The results showed that the proposed synthesis pathway resulted in the obtained of the chitosan biomaterials with high potential in medicine. [ABSTRACT FROM AUTHOR]

Tissue engineering is a branch of science that focuses on methods and techniques for the creation of new tissues and organs for the therapeutic reconstruction of the damaged organ by providing support structures, cells, molecular and mechanical signals for regeneration to the desired region. Conventional implants made of inert materials can eliminate only physical and mechanical defects of damaged tissues. The goal of tissue engineering is to restore biological functions, that is regeneration of tissues, and not only to replace it with a substitute made of synthetic material. The most important challenges of tissue engineering include the development of new biomaterials that will be used as three-dimensional scaffolds for cell cultures. Such scaffolding must be characterized by biocompatibility and biodegradability. The aim of the research was to obtain biomaterials based on acylated chitosan. The result of the work was to obtain three-dimensional scaffolding with bioactive properties based on raw materials of natural origin. The biomaterials were modified with ferrimagnetic nanoparticles which are capable of electromagnetic stimulation of proliferation. [ABSTRACT FROM AUTHOR]

Carbon Quantum Dots (CQDs) are objects with a size less than 10 nm that have the ability to emit radiation in the visible range from blue to red depending on the excitation radiation used. Quantum dots are used in in vitro bioimaging of cell structures or creation of biosensors. In contrast to classic nanodots, which are obtained from simple sulphides, selenides or metal tellurides, carbon quantum dots are constructed from a non-toxic, biocompatible carbon core, thanks to which it is possible to apply quantum carbon dots in bio-imaging in-vitro or in-vivo biological structures with minimal cytotoxic effect on cells. The aim of the research was to obtain carbon nanodots capable of emitting fluorescence using lignin from waste biomass. The CQDs were functionalized with amino-acids. The result of the work was to obtain a series of CQDs with advanced luminescence properties using hydrothermal and microwave assisted methods. Ready products were investigated over their cytotoxicity. [ABSTRACT FROM AUTHOR]

Tissue engineering provides alternative solutions to traditional transplantation. In this study a novel strategy of chitosan scaffolds obtainment based on selective O-crosslinking using Aspartic acid and the addition of TiO2 nanoparticles is presented. Prepared under microwave conditions biomaterials were of increased mechanical and thermal durability thanks to NPs presence comparing to pure chitosan. Moreover porous scaffolds maintained antimicrobial activity against S. Aureus and E. Coli. Biomaterials were susceptible to in vitro biodegradation and degradation. Hydrogels exhibited positive impact on proliferation activity of fibroblasts. Thus they may be applied as 3D scaffolds in tissue engineering focused on wound healing. [ABSTRACT FROM AUTHOR]

In this work we present a novel strategy for chitosan-based scaffolds. Chitosan is a versatile biopolymer obtained from waste biomass known of its favorable biological properties. Thus it can replace other polymers in the preparation of bioactive scaffolds. To increase its durability chitosan can be crosslinked into form of the hydrogel yet application of toxic crosslinkers may lead to loss of biocompability. Mesenchymal stem cells can be used in cell therapy for advanced wound treatment. However their culture requires special biomaterials application. In this article a novel microwave-assisted synthesis method for bioactive chitosan scaffolds is presented. [ABSTRACT FROM AUTHOR]

Nowadays, burn wounds are one of the most emerging problems of medicine. Tissue engineering seems to be a promising solution since it enables new tissue formation on 3 D scaffolds. In this article, a novel strategy for N-acylated chitosan 3 D scaffolds obtainment method is presented. Biomaterials were synthesized according to Green Chemistry principles under microwave-assisted conditions using unsaturated fatty acid chlorides. Ready products were characterized over their physicochemical properties, morphology, mechanical, and biological properties. Performed analyses confirmed obtainment of N-acylated biomaterial with a very porous structure and enhanced durability. Prepared scaffolds were biodegradable and had a positive impact on mouse fibroblasts. [ABSTRACT FROM AUTHOR]

Rapid regenerative medicine development requires new advanced biomaterials. Chitosan which is known of its unique properties like biocompability, biodegradability, bioactivity, antibacterial activity is a great candidate for scaffolds for cell culture. In this paper, a novel strategy for antioxidant aerogels obtainment method is presented. Biomaterials were synthesized under microwave-assisted conditions and modified with Tiliaplatyphyllos extract. Ready products were characterized over their physicochemical properties, morphology and biological properties including bioactivity and antimicrobial activity. Performed analyses confirmed obtainment of crosslinked biomaterial with advanced properties such as positive effect on cells proliferation and inhibition of Staphylococcus aureus growth. [ABSTRACT FROM AUTHOR]

A new type of promising chitosan beads with advanced properties were obtained under microwave radiation according to Green Chemistry principles. Biomaterials were prepared using chitosan as raw material and glutamic acid/1,5-pentanodiol mixture as crosslinking agents. Additionally beads were modified with Tilia platyphyllos extract to enhance their antioxidant properties. Beads were investigated over their chemical structure by FT-IR analysis. Also their morphology has been investigated by SEM method. Additionally swelling capacity of the obtained hydrogels was determined. Lack of cytotoxicity has been confirmed by MTT assay. Proliferation studies were carried out on L929 mouse fibroblasts. Advanced properties of the obtained beads were investigated by studying pH sensitivity and antioxidant properties by DPPH method. Also susceptibility to degradation and biodegradation by Sturm Test method was evaluated. Results shows that proposed chitosan beads and their eco-friendly synthesis method can be applied in cell therapy and tissue engineering. [ABSTRACT FROM AUTHOR]

Highlights: [•] All applied inducers triggered normoergic biochemical changes in potato leaves. [•] These changes might be a component of the stress imprint linked to the NO message. [•] GSNOR activity was modified in consequence of induced NO production. [•] Potato resistance was exhibited in hyperergic defense responses related to ROS generation. [•] BABA primed the most effective systemic protection against P. infestans. [Copyright &y& Elsevier]

Ischemic stroke is a disturbance in cerebral blood flow caused by brain tissue ischemia and hypoxia. We optimized a multifactorial in vitro model of acute ischemic stroke using rat primary neural cultures. This model was exploited to investigate the pro-viable activity of cell-penetrating peptides: arginine-rich Tat(49–57)-NH2 (R49KKRRQRRR57-amide) and its less basic analogue, PTD4 (Y47ARAAARQARA57-amide). Our model included glucose deprivation, oxidative stress, lactic acidosis, and excitotoxicity. Neurotoxicity of these peptides was excluded below a concentration of 50 μm, and PTD4-induced pro-survival was more pronounced. Circular dichroism spectroscopy and molecular dynamics (MD) calculations proved potential contribution of the peptide conformational properties to neuroprotection: in MD, Tat(49–57)-NH2 adopted a random coil and polyproline type II helical structure, whereas PTD4 adopted a helical structure. In an aqueous environment, the peptides mostly adopted a random coil conformation (PTD4) or a polyproline type II helical (Tat(49–57)-NH2) structure. In 30% TFE, PTD4 showed a tendency to adopt a helical structure. Overall, the pro-viable activity of PTD4 was not correlated with the arginine content but rather with the peptide's ability to adopt a helical structure in the membrane-mimicking environment, which enhances its cell membrane permeability. PTD4 may act as a leader sequence in novel drugs for the treatment of acute ischemic stroke. [ABSTRACT FROM AUTHOR]

Massive blood loss is still a great challenge for modern medicine. To stop the hemorrhage during the surgery or after injury apart from suturing or electrocoagulation, the most efficient method of hemostasis restoration is the use of hemostatic agents. Although there are numerous products on the market, there is still a need for biomaterials that are capable of fast and efficient bleeding management without affecting wound closure or embolism. Chitosan is known for its hemostatic activity; however, its quite poor mechanical properties and heterogenous chemical composition still needs some improvements to become superior compared to biological adhesives. The following study deals with the preparation and evaluation of chitosan-derived natural biomaterials containing Kalanchoe pinnata extract with the potential application as a blood-clotting agent. The materials were obtained under microwave-assisted conditions in two different forms (granules/dressing), whose chemical structure and morphology were studied. Their antioxidant properties have been proven. The chitosan-derived hemostatic agents exhibited superior blood sorption abilities and lack of cytotoxicity to L929 mouse fibroblasts. The study also showed the differences in biological properties depending on their preparation method. The potential mechanism of action was proposed as well as their potential in hemostasis revival. [ABSTRACT FROM AUTHOR]

One of the most common neurological diseases is epilepsy, which not only negatively affects the quality of people's life but also may lead to life-threatening situations when its symptoms such as seizures cannot be controlled medically. A very serious problem to be overcame is the untreatable form of this disease, which cannot be cured by any currently available medicines. Cannabidiol, which is a natural product obtained from Cannabis Sativa, brings a new hope to people suffering from drug-resistant epilepsy. However, the hydrophobic character of this compound significantly lowers its clinical efficiency. One of the promising methods of this substance bioactivity increase is delivery through the skin tissue. In this article, a new type of advanced transdermal systems based on chitosan and ZnO nanoparticles (NPs) has been developed according to Sustained Development principles. The chemical modification of the biopolymer confirmed by FT-IR method resulted in the preparation of the material with great swelling abilities and appropriate water vapor permeability. Obtained nanoparticles were investigated over their crystalline structure and morphology and their positive impact on drug loading capacity and cannabidiol controlled release was proved. The novel biomaterials were confirmed to have conductive properties and not be cytotoxic to L929 mouse fibroblasts. [ABSTRACT FROM AUTHOR]

Waste biomass such as lignin constitutes a great raw material for eco-friendly carbon quantum dots (CQDs) synthesis, which find numerous applications in various fields of industry and medicine. Carbon nanodots, due to their unique luminescent properties as well as water-solubility and biocompatibility, are superior to traditional organic dyes. Thus, obtainment of CQDs with advanced properties can contribute to modern diagnosis and cell visualization method development. In this article, a new type of coumarin-modified CQD was obtained via a hybrid, two-step pathway consisting of hydrothermal carbonization and microwave-assisted surface modification with coumarin-3-carboxylic acid and 7-(Diethylamino) coumarin-3-carboxylate. The ready products were characterized over their chemical structure and morphology. The nanomaterials were confirmed to have superior fluorescence characteristics and quantum yield up to 18.40%. They also possessed the ability of biomolecules and ion detection due to the fluorescence quenching phenomena. Their lack of cytotoxicity to L929 mouse fibroblasts was confirmed by XTT assay. Moreover, the CQDs were proven over their applicability in real-time bioimaging. Obtained results clearly demonstrated that proposed surface-modified carbon quantum dots may become a powerful tool applicable in nanomedicine and pharmacy. [ABSTRACT FROM AUTHOR]

• Chitosan hydrogels were obtained using AZA and ZnO nanorods under MW conditions. • Obtained nanocomposites were tested over their structure, morphology and durability. • Transdermal systems had excellent swelling abilities and water vapor permeability. • Matrixes were loaded with ASA which was released according to 1st order kinetics. • Transdermal delivery systems were non-toxic to L929 mouse fibroblasts. The growing number of people suffering from civilization diseases increases the amount of medication taken. Thus, novel methods for drug delivery must be developed which will constitute an alternative to oral administration. A new hope for patients bring transdermal drug delivery systems. To overcome skin barrier function, they must be prepared from materials which increase cell membrane permeability for the medication. Therefore, there is an increasing need for novel, advanced transdermal systems capable of controlled active substance release under specific stimuli. The aim of this research was to obtain novel hydrogel-based transdermal delivery systems through crosslinking process of chitosan using azelaic acid followed by doping with ZnO nanorods to enhance its drug sorption properties. Ready materials were investigated over their structure, morphology and durability. Drug loading capacity, controlled drug release ability and its kinetics were determined on medication used in treatment of cardiovascular system diseases - acetylsalicylic acid. Finally, lack of cytotoxicity was confirmed by XTT assay and cell morphology study carried out on L929 mouse fibroblasts. Obtained results show a great potential of the developed transdermal delivery systems in active substances administration through skin tissue and may help to protect digestive tract of the patients in the future. [ABSTRACT FROM AUTHOR]

The growing number of female reproductive system disorders creates a need for novel treatment methods. Tissue engineering brings hope for patients, which enables damaged tissue reconstruction. For this purpose, epithelial cells are cultured on three-dimensional scaffolds. One of the most promising materials is chitosan, which is known for its biocompatibility and biodegradability. The aim of the following study was to verify the potential of chitosan-based biomaterials for pelvic organ prolapse regeneration. The scaffolds were obtained under microwave-assisted conditions in crosslinking reactions, using dicarboxylic acids and aminoacid as crosslinkers, including l-glutamic acid, adipic acid, malonic acid, and levulinic acid. The products were characterized over their physicochemical and biological properties. FT–IR analysis confirmed formation of amide bonds. The scaffolds had a highly porous structure, which was confirmed by SEM analysis. Their porosity was above 90%. The biomaterials had excellent swelling abilities and very good antioxidant properties. The cytotoxicity study was performed on vaginal epithelial VK2/E6E7 and human colon cancer HCT116 cell lines. The results showed that after certain modifications, the proposed scaffolds could be used in pelvic organ prolapse (POP) treatment. [ABSTRACT FROM AUTHOR]

Recently, fluorescent probes became one of the most efficient tools for biosensing and bioimaging. Special attention is focused on carbon quantum dots (CQDs), which are characterized by the water solubility and lack of cytotoxicity. Moreover, they exhibit higher photostability comparing to traditional organic dyes. Currently, there is a great need for the novel, luminescent nanomaterials with tunable properties enabling fast and effective analysis of the biological samples. In this article, we propose a new, ecofriendly bottom-up synthesis approach for intelligent, surface-modified nanodots preparation using bioproducts as a raw material. Obtained nanomaterials were characterized over their morphology, chemical structure and switchable luminescence. Their possible use as a nanodevice for medicine was investigated. Finally, the products were confirmed to be non-toxic to fibroblasts and capable of cell imaging. [ABSTRACT FROM AUTHOR]

Bone tissue is the second tissue to be replaced. Annually, over four million surgical treatments are performed. Tissue engineering constitutes an alternative to autologous grafts. Its application requires three-dimensional scaffolds, which mimic human body environment. Bone tissue has a highly organized structure and contains mostly inorganic components. The scaffolds of the latest generation should not only be biocompatible but also promote osteoconduction. Poly (lactic acid) nanofibers are commonly used for this purpose; however, they lack bioactivity and do not provide good cell adhesion. Chitosan is a commonly used biopolymer which positively affects osteoblasts' behavior. The aim of this article was to prepare novel hybrid 3D scaffolds containing nanohydroxyapatite capable of cell-response stimulation. The matrixes were successfully obtained by PLA electrospinning and microwave-assisted chitosan crosslinking, followed by doping with three types of metallic nanoparticles (Au, Pt, and TiO2). The products and semi-components were characterized over their physicochemical properties, such as chemical structure, crystallinity, and swelling degree. Nanoparticles' and ready biomaterials' morphologies were investigated by SEM and TEM methods. Finally, the scaffolds were studied over bioactivity on MG-63 and effect on current-stimulated biomineralization. Obtained results confirmed preparation of tunable biomimicking matrixes which may be used as a promising tool for bone-tissue engineering. [ABSTRACT FROM AUTHOR]

Carbon quantum dots (CQDs) are nanoobjects of a size below 10 nm. Due to their favorable features, such as tunable luminescence, unique optical properties, water solubility, and lack of cytotoxicity, they are willingly applied in biomedicine. They can be obtained via bottom-up and top-down methods. However, to increase their quantum yield they must undergo post-processing. The aim of the following research was to obtain a new type of CQDs modified with a rhodamine b derivative to enhance their fluorescence performance without biocompability deterioration. For their preparation glucose was used as a precursor and four different carbonizing agents which affected semi- and final products luminescence properties. The ready nanomaterials were investigated over their chemical structure by FTIR and NMR, whereas morphology was investigated by the TEM method. Their optical properties were determined by UV–VIS spectroscopy. Fluorescence behavior, photo- and pH-stability, as well as solvatochromism showed their applicability in various biomedical applications due to the controlled properties. The samples exhibited excellent antioxidant activity and lack of cytotoxicity on L929 mouse fibroblasts. The results showed that proposed strategy enables preparation of the superior nanomaterials with outstanding luminescence properties such as quantum yield up to 17% which can be successfully applied in cell labelling, bioimaging, and theranostics. [ABSTRACT FROM AUTHOR]

Burns affect almost half a million of Americans annually. In the case of full-thickness skin injuries, treatment requires a transplant. The development of bioactive materials that promote damaged tissue regeneration constitutes a great alternative to autografts. For this reason, special attention is focused on three-dimensional scaffolds that are non-toxic to skin cells and can mimic the extracellular matrix, which is mainly composed of nanofibrous proteins. Electrospinning, which enables the preparation of nanofibers, is a powerful tool in the field of biomaterials. In this work, novel hybrid poly (lactic acid)/chitosan biomaterials functionalized with three types of nanoparticles (NPs) were successfully developed. ZnO, Fe3O4, and Au NPs were investigated over their morphology by TEM method. The top layer was obtained from PLA nanofibers, while the bottom layer was prepared from acylated chitosan. The layers were studied over their morphology by the SEM method and their chemical structure by FT-IR. To verify their potential in burn wound treatment, the scaffolds' susceptibility to biodegradation as well as moisture permeability were calculated. Also, biomaterials conductivity was determined in terms of electrostimulation. Finally, cytotoxicity tests were carried out by XTT assay and morphology analysis using both fibroblasts cell line and primary cells. The hybrid nanofibrous scaffolds displayed a great potential in tissue engineering. [ABSTRACT FROM AUTHOR]

Carbon nanomaterials in the form of quantum dots have a high potential due to their luminescent properties and low cytotoxicity which allows their use in optical probes for use in bioimaging and biodetection. In this article, we present a novel type of nanomaterials and their obtainment method under microwave-assisted conditions using poly(L-lysine) as a raw material. The ready products were characterized over their chemical structure, pH-dependent fluorescence properties and cytotoxicity on human dermal fibroblasts. Moreover, their antioxidant activity as well as ability to biologically active molecules (vitamins) and heavy metal ions detection was evaluated. The results confirmed the obtainment of biocompatible nanomaterials with advanced properties and good water solubility according to sustained development principles. [ABSTRACT FROM AUTHOR]

Massive blood loss is responsible for numerous causes of death. Hemorrhage may occur on the battlefield, at home or during surgery. Commercially available biomaterials may be insufficient to deal with excessive bleeding. Therefore novel, highly efficient hemostatic agents must be developed. The aim of the following research was to obtain a new type of biocompatible chitosan-based hemostatic agents with increased hemostatic properties. The biomaterials were obtained in a quick and efficient manner under microwave radiation using l-aspartic and l-glutamic acid as crosslinking agents with no use of acetic acid. Ready products were investigated over their chemical structure by FT-IR method which confirmed a crosslinking process through the formation of amide bonds. Their high porosity above 90% and low density (below 0.08 g/cm3) were confirmed. The aerogels were also studied over their water vapor permeability and antioxidant activity. Prepared biomaterials were biodegradable in the presence of human lysozyme. All of the samples had excellent hemostatic properties in contact with human blood due to the platelet activation confirmed by blood clotting tests. The SEM microphotographs showed the adherence of blood cells to the biomaterials' surface. Moreover, they were biocompatible with human dermal fibroblasts (HDFs). The biomaterials also had superior antibacterial properties against both Staphylococcus aureus and Escherichia coli. The obtained results showed that proposed chitosan-based hemostatic agents have great potential as a hemostatic product and may be applied under sterile, as well as contaminated conditions, by both medicals and individuals. [ABSTRACT FROM AUTHOR]

Rapid development in medicine and pharmacy has created a need for novel biomaterials with advanced properties such as photoluminescence, biocompability and long-term stability. The following research deals with the preparation of novel types of N-doped chitosan-based carbon quantum dots. Nanomaterials were obtained with simultaneous nitrogen-doping using biocompatible amino acids according to Green Chemistry principles. For the carbon quantum dots synthesis chitosan was used as a raw material known for its biocompability. The nanomaterials obtained in the form of lyophilic colloids were characterized by spectroscopic and spectrofluorimetric methods. Their quantum yields were determined. Additionally the cytotoxicity of the prepared bionanomaterials was evaluated by XTT (2,3-Bis-(2-methoxy-4-nitro5-sulfophenyl)-2H-tetrazolium-5-carboxanilide salt) method. Our results confirmed the formation of biocompatible quantum dots with carbon cores exhibiting luminescence in visible range. Performed studies showed that modification with lysine (11.5%) and glutamic acid (7.4%) had a high impact on quantum yield, whereas functionalization with amino acids rich in S and N atoms did not significantly increase in fluorescence properties. XTT assays as well as morphological studies on human dermal fibroblasts confirmed the lack of cytotoxicity of the prepared bionanomaterials. The study shows chitosan-based quantum dots to be promising for biomedical applications such as cell labelling, diagnostics or controlled drug delivery and release systems. [ABSTRACT FROM AUTHOR]

Abstract: The primed mobilization for more potent defense responses to subsequent stress has been shown for many plant species, but there is a growing need to identify reliable molecular markers for this unique phenomenon. In the present study a proteomic approach was used to screen similarities in protein abundance in leaves of primed potato (Solanum tuberosum L.) treated with four well-known inducers of plant resistance, i.e. β-aminobutyric acid (BABA), γ-aminobutyric acid (GABA), Laminarin and 2,6-dichloroisonicotinic acid (INA), respectively. Moreover, to gain insight into the importance of nitric oxide (NO) in primed protein accumulation the potato leaves were supplied by S-nitrosoglutathione (GSNO), as an NO donor. The comparative analysis, using two-dimensional electrophoresis and mass spectrometry, revealed that among 25 proteins accumulated specifically after BABA, GABA, INA and Laminarin treatments, 13 proteins were accumulated also in response to GSNO. Additionally, overlapping proteomic changes between BABA-primed and GSNO-treated leaves showed 5 protein spots absent in the proteome maps obtained in response to the other priming agents. The identified 18 proteins belonged, in most cases, to functional categories of primary metabolism. The selected proteins including three redox-regulated enzymes, i.e. glyceraldehyde 3-phosphate dehydrogenase, carbonic anhydrase, and fructose-bisphosphate aldolase, were discussed in relation to the plant defence responses. Taken together, the overlapping effects in the protein profiles obtained between priming agents, GSNO and cPTIO treatments provide insight indicating that the primed potato exhibits unique changes in the primary metabolism, associated with selective protein modification via NO. [Copyright &y& Elsevier]

Regeneration and wound healing are vital to tissue homeostasis and organism survival. One of the biggest challenges of today's science and medicine is finding methods and factors to stimulate these processes in the human body. Effective solutions to promote regenerative responses will accelerate advances in tissue engineering, regenerative medicine, transplantology, and a number of other clinical specialties. In this study, we assessed the potential efficacy of a synthetic hexapeptide, RDKVYR, for the stimulation of tissue repair and wound healing. The hexapeptide is marketed under the name "Imunofan" (IM) as an immunostimulant. IM displayed stability in aqueous solutions, while in plasma it was rapidly bound by albumins. Structural analyses demonstrated the conformational flexibility of the peptide. Tests in human fibroblast and keratinocyte cell lines showed that IM exerted a statistically significant ( p < 0.05) pro-proliferative activity (30-40% and 20-50% increase in proliferation of fibroblast and keratinocytes, respectively), revealed no cytotoxicity over a vast range of concentrations ( p < 0.05), and had no allergic properties. IM was found to induce significant transcriptional responses, such as enhanced activity of genes involved in active DNA demethylation ( p < 0.05) in fibroblasts and activation of genes involved in immune responses, migration, and chemotaxis in adipose-derived stem cells derived from surgery donors. Experiments in a model of ear pinna injury in mice indicated that IM moderately promoted tissue repair (8% in BALB/c and 36% in C57BL/6 in comparison to control).

Applications of bioactive peptides and polypeptides are emerging in areas such as drug development and drug delivery systems. These compounds are bioactive, biocompatible and represent a wide range of chemical properties, enabling further adjustments of obtained biomaterials. However, delivering large quantities of peptide derivatives is still challenging. Several methods have been developed for the production of concatemers - multiple copies of the desired protein segments. We have presented an efficient method for the production of peptides of desired length, expressed from concatemeric Open Reading Frame. The method employs specific amplification-expression DNA vectors. The main methodological approaches are described by Skowron et al., 2020 [1]. As an illustration of the demonstrated method's utility, an epitope from the S protein of Hepatitis B virus (HBV) was amplified. Additionally, peptides, showing potentially pro-regenerative properties, derived from the angiopoietin-related growth factor (AGF) were designed and amplified. Here we present a dataset including: ( i ) detailed protocols for the purification of HBV and AGF - derived polyepitopic protein concatemers, ( ii ) sequences of the designed primers, vectors and recombinant constructs, ( iii ) data on cytotoxicity, immunogenicity and stability of AGF-derived polypeptides., (© 2019 The Author(s).)