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Laser microstructuring has been studied extensively in the last decades due to its versatile, contactless processing and outstanding precision and structure quality on a wide range of materials. A limitation of the approach has been identified in the utilization of high average laser powers, with scanner movement fundamentally limited by laws of inertia. In this work, we apply a nanosecond UV laser working in an intrinsic pulse-on-demand mode, ensuring maximal utilization of the fastest commercially available galvanometric scanners at scanning speeds from 0 to 20 m/s. The effects of high-frequency pulse-on-demand operation were analyzed in terms of processing speeds, ablation efficiency, resulting surface quality, repeatability, and precision of the approach. Additionally, laser pulse duration was varied in single-digit nanosecond pulse durations and applied to high throughput microstructuring. We studied the effects of scanning speed on pulse-on-demand operation, single- and multipass laser percussion drilling performance, surface structuring of sensitive materials, and ablation efficiency for pulse durations in the range of 1-4 ns. We confirmed the pulse-on-demand operation suitability for microstructuring for a range of frequencies from below 1 kHz to 1.0 MHz with 5 ns timing precision and identified the scanners as the limiting factor even at full utilization. The ablation efficiency was improved with longer pulse durations, but structure quality degraded.

The improvement of mechanical properties of polymer-based nanocomposites is usually obtained through a strong polymer-silica interaction. Most often, precipitated silica nanoparticles are used as filler. In this work, we study the synergetic effect occurring between dual silica-based fillers in a styrene-butadiene rubber (SBR)/polybutadiene (PBD) rubber matrix. Precipitated Highly Dispersed Silica (HDS) nanoparticles (10 nm) have been associated with spherical Stöber silica nanoparticles (250 nm) and anisotropic nano-Sepiolite. By imaging filler at nano scale through Scanning Transmission Electron Microscopy, we have shown that anisotropic fillers align only in presence of a critical amount of HDS. The dynamic mechanical analysis of rubber compounds confirms that this alignment leads to a stiffer nanocomposite when compared to Sepiolite alone. On the contrary, spherical 250 nm nanoparticles inhibit percolation network and reduce the nanocomposite stiffness.

The deposition of polydopamine (PDA) films on surfaces, a versatile deposition method with respect to the nature of the used substrate, is unfortunately accompanied by deposition of insoluble precipitates in solution after a prolonged oxidation time of dopamine solutions. Therefore, there is evident interest to find methods able to stop the deposition of PDA on surfaces and to simultaneously control the self-assembly of PDA in solution to get stable colloidal aggregates. In addition to proposed methods relying on the use of polymers like poly(vinyl alcohol) and proteins like human serum albumin, we show herein that boric acid is an efficient adjuvant that is simultaneously able to stop the self-assembly of PDA in solution as well as on surfaces and to change the adhesive properties of the resulting PDA coatings.

A two-stage master-oscillator power-amplifier (MOPA) system based on Yb:YAG single-crystal-fiber (SCF) technology and designed for high peak power is studied to significantly increase the pulse energy of a low-power picosecond laser. The first SCF amplifier has been designed for high gain. Using a gain medium optimized in terms of doping concentration and length, an optical gain of 32 dB has been demonstrated. The second amplifier stage designed for high energy using the divided pulse technique allows us to generate a recombined output pulse energy of 2 mJ at 12.5 kHz with a pulse duration of 6 ps corresponding to a peak power of 320 MW. Average powers ranging from 25 to 55 W with repetition rates varying from 12.5 to 500 kHz have been demonstrated.

We demonstrate a three-stage diode-pumped Yb:YAG single-crystal-fiber amplifier to generate femtosecond pulses at high average powers with linear or cylindrical (i.e., radial or azimuthal) polarization. At a repetition rate of 20 MHz, 750-fs pulses were obtained at an average power of 85 W in cylindrical polarization and at 100 W in linear polarization. The report includes investigations on the use of Yb:YAG single-crystal fibers with different length/doping ratio and the zero-phonon pumping at a wavelength of 969 nm in order to optimize the performance.

We demonstrate a deep-UV laser at 236.5 nm based on extracavity fourth-harmonic generation of a Q-switched Nd:YAG single-crystal fiber laser at 946 nm. We first compare two nonlinear crystals available for second-harmonic generation: LBO and BiBO. The best results at 473 nm are obtained with a BiBO crystal, with an average output power of 3.4 W at 20 kHz, corresponding to a second-harmonic generation efficiency of 38%. This blue laser is frequency-converted to 236.5 nm in a BBO crystal with an overall fourth-harmonic generation yield of 6.5%, corresponding to an average output power of 600 mW at 20 kHz. This represents an order of magnitude increase in average power and energy compared to previously reported pulsed lasers at 236.5 nm. This work opens the possibility of LIDAR detection of dangerous compounds for military or civilian applications.

A resonant grating mirror (RGM) that combines a single layer planar waveguide and a subwavelength grating is used to simultaneously control the beam quality, the spectral bandwidth, and the polarization state of an Er:YAG laser. This simple device is compared to classical methods using several intracavity components: an etalon for wavelength selection, a thin film polarizer for polarization selection, and an aperture for spatial filtering. It is demonstrated that the RGM provides the same polarization purity, an enhanced spectral filtering, and a significant improvement of the beam quality. In CW operation, the Er:YAG laser with a RGM emits an output power of 1.4 W at 1617 nm with a M2 of 1.4.

We present unique spatial-mode switching in a cw Yb:CALGO laser when pumped at a multihundred-watts power level. It permits us to automatically stabilize to a TEM(00) mode from a highly spatial multimode regime. This stabilization is achievable thanks to polarization-mode switching allowed by the particular spectroscopic and thermal properties of Yb:CALGO crystal. This atypical and unexpected behavior is studied in detail in this Letter and explained by analysis of the thermo-optical coefficients for CALGO.

We report the realization of a frequency doubled, actively Q-switched and polarized oscillator based on Nd:YAG single-crystal fiber. A laser output of 8 W, 10 kHz, and 30 ns at 946 nm is reported. The laser is extracavity frequency doubled in a BiBO crystal to obtain 3 W and 300 μJ of blue laser with a beam quality of M(2)y=1.12 and M(2)x=1.38. The obtained blue power is stable with a root-mean-square stability of less than 2% in 1 h. This is more than two times the previously reported average power and energy at 473 nm.

Yb:YAG single crystal fiber (SCF) amplifiers have recently drawn much attention in the field of amplification of ultra-short pulses. In this paper, we report on the use of SCF amplifiers for the amplification of cylindrically polarized laser beams, as such beams offer promising properties for numerous applications. While the amplification of cylindrically polarized beams is challenging with other amplifier designs due to thermally induced depolarization, we demonstrate the amplification of 32 W cylindrically polarized beams to an output power of 100 W. A measured degree of radial polarization after the SCF of about 95% indicates an excellent conservation of polarization.

We demonstrated laser operation of a passively Q-switched diode-pumped Er:YAG solid-state laser emitting at 1645 or 1617 nm depending on the initial transmission of the Cr:ZnSe saturable absorber. The crystal emitted up to 10 W at 1645 nm and up to 8 W at 1617 nm in CW mode while pumped with 65 W of incident pump power at 1533 nm. In passive Q-switched mode with 40 W of incident power, a Cr:ZnSe saturable absorber with initial transmission of 85% led to 330 μJ pulse energy, 61 ns pulse duration at a repetition rate of 1460 Hz at 1645 nm. An 80% initial transmission Cr:ZnSe sample led to 510 μJ energy pulses, 41 ns pulse duration at a repetition rate of 820 Hz with a central wavelength change from 1645 to 1617 nm. This is the first reported passively Q-switched diode-pumped Er:YAG laser operating on the (4)I(13/2)→(4)I(15/2) transition.

We report on a high-power narrow-linewidth pulsed laser source emitting at a wavelength of 257 nm. The system is based on a master oscillator power amplifier architecture, with Yb-doped fiber preamplifiers, a Yb:YAG single crystal fiber power amplifier used to overcome the Brillouin limitation in glass fiber and nonlinear frequency conversion stages. This particularly versatile architecture allows the generation of Fourier transform-limited 15 ns pulses at 1030 nm with 22 W of average power and a diffraction-limited beam (M(2)<1.1). At a repetition rate of 30 kHz, 106 μJ UV pulses are generated corresponding to an average power of 3.2 W.

We demonstrate a versatile femtosecond power amplifier using a Yb:YAG single crystal fiber operating from 10 kHz to 10 MHz. For a total pump power of 75 W, up to 30 W is generated from the double-pass power amplifier. At a repetition rate of 10 kHz, an output energy of 1 mJ is obtained after recompression. In this configuration, the pulse duration is 380 fs, corresponding to a peak power of 2.2 GW. The M2 beam quality factor is better than 1.1 for investigated parameters.

We demonstrate an Yb:YAG single-crystal fiber laser with 251 W output power in continuous-wave and an optical efficiency of 44%. This performance can be explained by the high overlap between pump and signal beams brought by the pump guiding and by the good thermal management provided by the single-crystal fiber geometry. The oscillator performance with a reflectivity of the output coupler as low as 20% also shows high potential for power amplification.

We explore the potential of Nd:YAG single-crystal fibers for the amplification of passively Q-switched microlasers operating below 1 ns. Different regimes are tested in single or double pass configurations. For high gain and high power amplification this novel gain medium provided average powers up to 20 W at high repetition rate (over 40 kHz) for a pulse duration of 1 ns. As an energy amplifier, Nd:YAG single-crystal fiber delivered 2.7 mJ, 6 MW 450 ps pulses at 1 kHz. The extraction efficiencies vary from 8% to 32.7% following the configurations.

We demonstrated that Yb:YAG single crystal fibers have a strong potential for the amplification of femtosecond pulses. Seeded by 230 fs pulses with an average power of 400 mW at 30 MHz delivered by a passively mode-locked Yb:KYW oscillator, the system produced 330 fs pulses with an average power of 12 W. This is the shortest pulse duration ever produced by an Yb:YAG amplifier. The gain in the single crystal fiber reached a value as high as 30 in a simple double-pass configuration.

We report an in situ thermal study of Yb-doped fluorite crystals Yb:CaF(2) and Yb:SrF(2) under high power pumping, with or without laser operation. The experiment combines simultaneously thermography and measurement of the thermal aberrations. This setup allows us to measure temperature gradients, thermal lens, and absorption coefficients. From these measurements, we evaluate the thermal conductivity, fractional thermal load, and thermo-optic coefficient. Great differences are observed between the lasing and non lasing regimes. Our measured thermal lenses are greater than what are expected from the thermo-optic parameters found in previous work. Based on this thermal study, we design a laser cavity operating with large output power and TEM(00), leading to better performances for Yb:CaF(2) than Yb:SrF(2).

We present a thermal conductivity measurement method for laser crystals based on thermal mapping of the crystal face by an infrared camera. Those measurements are performed under end-pumping of the laser crystal and during laser operation. The calculation of the fraction of pump power converted into heat is therefore simplified, and it is possible to link easily the temperature in the crystal to the thermal conductivity. We demonstrate the efficiency of this measurement method with a Nd:YAG crystal, before using it to compare Nd:YVO(4) and Nd:GdVO(4) crystals.

We present optical characterization and laser results achieved with single-crystal fibers directly grown by the micro-pulling-down technique. We investigate the spectroscopic and optical quality of the fiber, and we present the first laser results. We achieved a cw laser power of 10 W at 1064 nm for an incident pump power of 60 W at 808 nm and 360 kW peak power for 12 ns pulses at 1 kHz in the Q-switched regime. It is, to the best of our knowledge, the highest laser power ever achieved with directly grown single-crystal fibers.

Metal ions are essential for DNA polymerase and RNase H activities of HIV-1 reverse transcriptase (RT). RT studies are routinely performed at 6-8 mM Mg2+, despite the fact that the in vivo concentration might be as low as 0.2 mM. We studied the influence of MgCl2 and ATP, which likely binds a significant fraction of the magnesium pool in vivo, on the DNA polymerase and RNase H activities of HIV-1 RT, its inhibition by nucleoside RT inhibitors (NRTIs) and primer unblocking by AZT-resistant RT. At low Mg2+ concentration, reverse transcription of a natural template strongly increased despite a dramatically reduced intrinsic polymerase activity under such conditions. Low Mg2+ concentrations affected the RNA stability and indirectly decreased its degradation by the RNase H activity. The reduced RNA degradation prevented premature dissociation of the template and primer strands that otherwise generated dead-end DNA products. In addition, low Mg2+ dramatically decreased the incorporation of NRTIs into DNA and increased nucleotide excision by AZT-resistant RT. The latter effect is also most likely owing to the diminished cleavage of the RNA template. Thus, differences in the free Mg2+ concentration between different cell types or during the cell cycle might strongly affect HIV-1 replication and its inhibition.

HIV-1 reverse transcriptase (RT) is one of the main targets for antiviral therapy. Two classes of RT inhibitors can be distinguished: those that are nucleoside or nucleotide analogues (sharing the common NRTIs abbreviation) and those that are not. This review focuses on the NRTIs, which are highly efficient in slowing down viral replication and are used in combination regimens. Unfortunately, the current inhibitors do not completely suppress viral replication and due to the high capacity of adaptation of HIV, allow the selection of drug-resistant viruses. Resistance mechanisms to NRTIs have been extensively investigated and can be divided into two types: improved discrimination of a nucleotide analogue relative to the natural substrate or increased phosphorolytic cleavage of an analogue-blocked primer. This knowledge is important both for the development of new drugs designed to target resistant strains and for the development of new antiviral strategies. The NRTIs currently in clinical trials and new developments in this area are also reviewed.

The experimental demonstration of a diode-pumped passively mode-locked femtosecond laser based on an Yb3+:CaGdAlO4 single crystal is reported. The oscillator is directly diode pumped by a high-brightness 5 W fiber coupled laser diode, and pulses are produced by use of a semiconductor saturable-absorber mirror. It permits the production of pulses as short as 47 fs at 1050 nm, which are to our knowledge the shortest laser pulses obtained from an oscillator based on Yb3+-doped bulk materials. The average power is 38 mW, and the repetition rate is 109 MHz.

Human immunodeficiency virus type I reverse transcriptase (RT) possesses distinct DNA polymerase and RNase H sites, whereas integrase (IN) uses the same active site to perform 3'-end processing and strand transfer of the proviral DNA. These four enzymatic activities are essential for viral replication and require metal ions. Two Mg2+ ions are present in the RT polymerase site, and one or two Mg2+ ions are required for the catalytic activities of RNase H and IN. We tested the possibility of inhibition of the RT polymerase and RNase H as well as the IN 3'-end processing and transfer activities of purified enzymes by a series of 3,7-dihydroxytropolones designed to target two Mg2+ ions separated by approximately 3.7 angstroms. The RT polymerase and IN 3' processing and strand transfer activities were inhibited at submicromolar concentrations, while the RNase H activity was inhibited in the low micromolar range. In all cases, the lack of inhibition by tropolones and O-methylated 3,7-dihydroxytropolones was consistent with the active molecules binding the metal ions in the active site. In addition, inhibition of the DNA polymerase activity was shown to depend on the Mg2+ concentration. Furthermore, selective inhibitors were identified for several of the activities tested, leaving some potential for design of improved inhibitors. However, all tested compounds exhibited cellular toxicity that presently limits their applications.