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Your search keyword '"Iwata, Satoru"' showing total 242 results
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242 results on '"Iwata, Satoru"'

4. A Recql5 mutant facilitates complex CRISPR/Cas9-mediated chromosomal engineering in mouse zygotes.

Author

Iwata, Satoru, Nagahara, Miki, Ido, Risako, and Iwamoto, Takashi

Subjects
*HUMAN abnormality genetics, *BIOLOGICAL models, *RESEARCH funding, *TISSUE engineering, *DNA, *TRANSCRIPTION factors, *CHROMOSOME abnormalities, *GENES, *MICE, *ANIMAL experimentation, *GENETIC mutation, TUMOR genetics
Abstract

Complex chromosomal rearrangements (CCRs) are often observed in clinical samples from patients with cancer and congenital diseases but are difficult to induce experimentally. Here, we report the first success in establishing animal models for CCRs. Mutation in Recql5 , a crucial member of the DNA helicase RecQ family involved in DNA replication, transcription, and repair, enabled CRISPR/Cas9-mediated CCRs, establishing a mouse model containing triple fusion genes and megabase-sized inversions. Some of these structural features of individual chromosomal rearrangements use template switching and microhomology-mediated break-induced replication mechanisms and are reminiscent of the newly described phenomenon "chromoanasynthesis." These data show that Recql5 mutant mice could be a powerful tool to analyze the pathogenesis of CCRs (particularly chromoanasynthesis) whose underlying mechanisms are poorly understood. The Recql5 mutants generated in this study are to be deposited at key animal research facilities, thereby making them accessible for future research on CCRs. [ABSTRACT FROM AUTHOR]

Published
2024
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22. The small GTPase ARF-1.2 is a regulator of unicellular tube formation in Caenorhabditis elegans

Author

Kage-Nakadai, Eriko, Sun, Simo, Iwata, Satoru, Yoshina, Sawako, Nishikawa, Yoshikazu, and Mitani, Shohei

Subjects
Caenorhabditis elegans, Psychology and mental health
Abstract

The membrane trafficking events that regulate unicellular tube formation and maintenance are not well understood. Here, using an RNAi screen, we identified the small GTPase ARF1 homolog ARF-1.2 as a regulator of excretory tube formation in Caenorhabditis elegans. RNAi-mediated knockdown and knockout of the arf-1.2 gene resulted in the formation of large intracellular vacuoles at the growth sites (varicosities) of the excretory canals. arf-1.2 mutant animals were sensitive to hyperosmotic conditions. arf-1.2 RNAi affected the localization of the anion transporter SULP-8, which is expressed in the basal plasma membrane of the excretory canals, but did not affect the expression of SULP-4, which is expressed in the apical membrane. The phenotype of arf-1.2 mutants was suppressed by mutation of the small Rho GTPase CDC-42, a regulator of apical/basal traffic balance. These results suggest that ARF-1.2 plays an essential role in basal membrane traffic to regulate the formation of the unicellular excretory tube. Keywords: Caenorhabditis elegans, Unicellular tube formation, Small GTPase, ARF-1.2, CDC-42, Author(s): Eriko Kage-Nakadai[sup.1,2], Simo Sun[sup.2], Satoru Iwata[sup.1], Sawako Yoshina[sup.1], Yoshikazu Nishikawa[sup.2] and Shohei Mitani[sup.1] Introduction Epithelial tubes that enable nutrition uptake and fluid transport are essential in all metazoans. Tube [...]

Published
2018
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36. 3D FEM analysis of effect of current distribution on AC loss in shield layers of multi-layered HTS power cable

Author

Miyagi, Daisuke, Iwata, Satoru, Takahashi, Norio, and Torii, Shinji

Subjects
Shielding (Electricity) -- Analysis, Finite element method -- Usage, High temperature superconductors -- Electric properties, Business, Electronics, Electronics and electrical industries
Abstract

Three-dimensional (3-D) hexahedral finite element method (FEM) was used for examining the current distribution and AC loss in each shield layer of the co-axial multi-layered High Temperature Superconducting (HTS) power cable. Findings revealed that the current distribution in the shield layers can be controlled by adjusting the pitches of shield layers.

Published
2007

42. The shielding effect of HTS power cable based on E-J power law

Author

Miyagi, Daisuke, Iwata, Satoru, Wakatsuki, Tomohiro, Takahashi, Norio, Torii, Shinji, Ueda, Kiyotaka, and Yasuda, Kenji

Subjects
Electrical cables -- Properties, High temperature superconductivity -- Analysis, Business, Electronics, Electronics and electrical industries
Abstract

An experiment is conducted for analysing the current distribution in high T(sub c) superconducting (HTS) power cable based on E-J power law. The results show that the AC losses in shield layer with intervals are increased when the interval between wires becomes large.

Published
2004

50. Finding Maximum Edge-Disjoint Paths Between Multiple Terminals

Author

Iwata, Satoru and Yokoi, Yu

Subjects
FOS: Computer and information sciences, Discrete Mathematics (cs.DM), 90C27, 05C38, 05C21, 68Q25, Computer Science - Data Structures and Algorithms, Data Structures and Algorithms (cs.DS), MathematicsofComputing_DISCRETEMATHEMATICS, Computer Science - Discrete Mathematics
Abstract

Let $G=(V,E)$ be a multigraph with a set $T\subseteq V$ of terminals. A path in $G$ is called a $T$-path if its ends are distinct vertices in $T$ and no internal vertices belong to $T$. In 1978, Mader showed a characterization of the maximum number of edge-disjoint $T$-paths. In this paper, we provide a combinatorial, deterministic algorithm for finding the maximum number of edge-disjoint $T$-paths. The algorithm adopts an augmenting path approach. More specifically, we utilize a new concept of short augmenting walks in auxiliary labeled graphs to capture a possible augmentation of the number of edge-disjoint $T$-paths. To design a search procedure for a short augmenting walk, we introduce blossoms analogously to the matching algorithm of Edmonds (1965). When the search procedure terminates without finding a short augmenting walk, the algorithm provides a certificate for the optimality of the current edge-disjoint $T$-paths. From this certificate, one can obtain the Edmonds--Gallai type decomposition introduced by Seb\H{o} and Szeg\H{o} (2004). The algorithm runs in $O(|E|^2)$ time, which is much faster than the best known deterministic algorithm based on a reduction to linear matroid parity. We also present a strongly polynomial algorithm for the maximum integer free multiflow problem, which asks for a nonnegative integer combination of $T$-paths maximizing the sum of the coefficients subject to capacity constraints on the edges., Comment: 37 pages, 9 figures

Published
2019

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