1. Development of a Love-wave Immunosensor with Microfluidic Technology to Detect Phages in Dynamic Mode
- Author
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Matatagui, D., Fernandez, M., Fontecha, J., Santos, J., Horrillo, M., Moynet, D., Esquivel, J., Gracia, I., Cane, C., Dejous, C., Rebière, D., Institute of Applied Physics (IFA - CSIC), GRIDSEN, Parasitologie/Médicaments (PPF), Université Bordeaux Segalen - Bordeaux 2, Centro Nacional de Microelectronica [Spain] (CNM), Laboratoire de l'intégration, du matériau au système (IMS), and Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Université Sciences et Technologies - Bordeaux 1
- Subjects
viruses ,[SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics - Abstract
International audience; The goal has been to develop an immunosensor based on a Love-wave device and a PDMS microfluidic chip in order to detect potentially pathogenic microorganisms in real time. Therefore, it has been tested with the phage M13 detected through the mouse monoclonal antibody anti-M13 (AM13). The Double-layer Agar (DLA) technique has been used to quantify the number of phages, linked to the sensor surface, in plaques-forming unit (pfu); that has allowed us to compare the number of phages determined by DLA and the estimated by the frequency shift, and both were very similar. With the aim to obtain a short response time, the detection of the phage was carried out in dynamic mode. The range tested concentrations has varied between 2x10^10 pfu*ml^-1 and 5x10^8 pfu*ml^-1. A fast response and a good discrimination among concentrations have been achieved. Besides, the AM13 has been used as secondary antibody in order to measure lower concentrations, such as 5*10^7 pfu*ml^-1.
- Published
- 2012