1. Reverse line blot hybridisation screening of Pseudallescheria/Scedosporium species in patients with cystic fibrosis
- Author
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Q, Lu, A H G Gerrits, van den Ende, G S, de Hoog, R, Li, I, Accoceberry, I, Durand-Joly, J-P, Bouchara, F, Hernandez, L, Delhaes, Univ Angers, Okina, Peking University [Beijing], CBS-KNAW Fungal Biodiversity Centre, Institute of the Royal Netherlands Academy of Arts and Sciences, University of Amsterdam [Amsterdam] (UvA), Hôpital Saint-André, Centre Hospitalier Dunkerque, Groupe d'Étude des Interactions Hôte-Pathogène (GEIHP), Université d'Angers (UA), Laboratoire de Parasitologie-Mycologie [CHU Angers], Centre Hospitalier Universitaire d'Angers (CHU Angers), PRES Université Nantes Angers Le Mans (UNAM)-PRES Université Nantes Angers Le Mans (UNAM), Service de Parasitologie-Mycologie [CHRU LIlle], Institut de Microbiologie [CHRU Lille], Pôle de Biologie Pathologie Génétique [CHU Lille], Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Pôle de Biologie Pathologie Génétique [CHU Lille], Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), This work was funded by Special Scientific Research Project and Public Welfare Project of Health Profession of China, 11th Five‐year key special subject for Sci & Tech Research of China and China Scholarship Council., Evolutionary Biology (IBED, FNWI), Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur de Lille, and Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)
- Subjects
MESH: Pseudallescheria / genetics ,MESH: Humans ,[SDV]Life Sciences [q-bio] ,MESH: Mycoses / complications ,Nucleic Acid Hybridization ,Reproducibility of Results ,MESH: Mycoses / diagnosis ,Sensitivity and Specificity ,Cystic fibrosis ,MESH: Sensitivity and Specificity ,Pseudallescheria ,[SDV] Life Sciences [q-bio] ,MESH: Reproducibility of Results ,MESH: Scedosporium / genetics ,Mycoses ,MESH: Nucleic Acid Hybridization ,Humans ,Scedosporium ,MESH: Pseudallescheria / isolation & purification ,MESH: Cystic Fibrosis / complications ,MESH: Scedosporium / isolation & purification ,reverse line blot hybridisation - Abstract
International audience; The PCR-RLB (reverse line blot hybridisation) was applied as a molecular technique for the detection of members of Pseudallescheria and Scedosporium from sputum of patients with cystic fibrosis (CF). Fifty-nine sputum samples were collected from 52 CF patients, which were analysed by culture and PCR-RLB. Conventional and semi-selective culture yielded five positive samples, but the PCR-RLB hybridisation assay permitted the detection of members of Pseudallescheria/Scedosporium in 32 out of 52 patients (61.5%). In total, PCR-RLB yielded 47 positives. Pseudallescheria apiosperma was detected in 20 samples, while Pseudallescheria boydii and Pseudallescheria aurantiacum were detected in 17 and eight samples, respectively. Six samples gave a positive reaction with two distinct species-specific probes and one sample with three probes. In conclusion, the PCR-RLB assay described in this study allows the detection of Scedosporium spp. in CF sputum samples and the identification of Pseudallescheria apiosperma, P. boydii, S. aurantiacum, Scedosporium prolificans and Pseudallescheria minutispora.
- Published
- 2011