14 results on '"Horacio Esteban Hopp"'
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2. Morphological and genetic diversity of maize landraces along an altitudinal gradient in the Southern Andes.
- Author
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Juan Gabriel Rivas, Angela Veronica Gutierrez, Raquel Alicia Defacio, Jorge Schimpf, Ana Laura Vicario, Horacio Esteban Hopp, Norma Beatriz Paniego, and Veronica Viviana Lia
- Subjects
Medicine ,Science - Abstract
Maize (Zea mays ssp. mays) is a major cereal crop worldwide and is traditionally or commercially cultivated almost all over the Americas. The North-Western Argentina (NWA) region constitutes one of the main diversity hotspots of the Southern Andes, with contrasting landscapes and a large number of landraces. Despite the extensive collections performed by the "Banco Activo de Germoplasma INTA Pergamino, Argentina" (BAP), most of them have not been characterized yet. Here we report the morphological and molecular evaluation of 30 accessions collected from NWA, along an altitudinal gradient between 1120 and 2950 meters above sea level (masl). Assessment of morphological variation in a common garden allowed the discrimination of two groups, which differed mainly in endosperm type and overall plant size. Although the groups retrieved by the molecular analyses were not consistent with morphological clusters, they showed a clear pattern of altitudinal structuring. Affinities among accessions were not in accordance with racial assignments. Overall, our results revealed that there are two maize gene pools co-existing in NWA, probably resulting from various waves of maize introduction in pre-Columbian times as well as from the adoption of modern varieties by local farmers. In conclusion, the NWA maize landraces preserved at the BAP possess high morphological and molecular variability. Our results highlight their potential as a source of diversity for increasing the genetic basis of breeding programs and provide useful information to guide future sampling and conservation efforts.
- Published
- 2022
- Full Text
- View/download PDF
3. Citrus Genetic Transformation: An Overview of the Current Strategies and Insights on the New Emerging Technologies
- Author
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Gabriela Conti, Beatriz Xoconostle-Cázares, Gabriel Marcelino-Pérez, Horacio Esteban Hopp, and Carina A. Reyes
- Subjects
citrus transgenic plants ,in vitro regeneration ,transformation methods ,CRISPR in citrus ,reporter and selection markers ,cisgenesis and intragenesis ,Plant culture ,SB1-1110 - Abstract
Citrus are among the most prevailing fruit crops produced worldwide. The implementation of effective and reliable breeding programs is essential for coping with the increasing demands of satisfactory yield and quality of the fruit as well as to deal with the negative impact of fast-spreading diseases. Conventional methods are time-consuming and of difficult application because of inherent factors of citrus biology, such as their prolonged juvenile period and a complex reproductive stage, sometimes presenting infertility, self-incompatibility, parthenocarpy, or polyembryony. Moreover, certain desirable traits are absent from cultivated or wild citrus genotypes. All these features are challenging for the incorporation of the desirable traits. In this regard, genetic engineering technologies offer a series of alternative approaches that allow overcoming the difficulties of conventional breeding programs. This review gives a detailed overview of the currently used strategies for the development of genetically modified citrus. We describe different aspects regarding genotype varieties used, including elite cultivars or extensively used scions and rootstocks. Furthermore, we discuss technical aspects of citrus genetic transformation procedures via Agrobacterium, regular physical methods, and magnetofection. Finally, we describe the selection of explants considering young and mature tissues, protoplast isolation, etc. We also address current protocols and novel approaches for improving the in vitro regeneration process, which is an important bottleneck for citrus genetic transformation. This review also explores alternative emerging transformation strategies applied to citrus species such as transient and tissue localized transformation. New breeding technologies, including cisgenesis, intragenesis, and genome editing by clustered regularly interspaced short palindromic repeats (CRISPR), are also discussed. Other relevant aspects comprising new promoters and reporter genes, marker-free systems, and strategies for induction of early flowering, are also addressed. We provided a future perspective on the use of current and new technologies in citrus and its potential impact on regulatory processes.
- Published
- 2021
- Full Text
- View/download PDF
4. Snakin-1 affects reactive oxygen species and ascorbic acid levels and hormone balance in potato.
- Author
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Vanesa Nahirñak, Máximo Rivarola, Natalia Inés Almasia, María Pilar Barrios Barón, Horacio Esteban Hopp, Denis Vile, Norma Paniego, and Cecilia Vazquez Rovere
- Subjects
Medicine ,Science - Abstract
Snakin-1 is a member of the Solanum tuberosum Snakin/GASA family. We previously demonstrated that Snakin-1 is involved in plant defense to pathogens as well as in plant growth and development, but its mechanism of action has not been completely elucidated yet. Here, we showed that leaves of Snakin-1 silenced potato transgenic plants exhibited increased levels of reactive oxygen species and significantly reduced content of ascorbic acid. Furthermore, Snakin-1 silencing enhanced salicylic acid content in accordance with an increased expression of SA-inducible PRs genes. Interestingly, gibberellic acid levels were also enhanced and transcriptome analysis revealed that a large number of genes related to sterol biosynthesis were downregulated in these silenced lines. Moreover, we demonstrated that Snakin-1 directly interacts with StDIM/DWF1, an enzyme involved in plant sterols biosynthesis. Additionally, the analysis of the expression pattern of PStSN1::GUS in potato showed that Snakin-1 is present mainly in young tissues associated with active growth and cell division zones. Our comprehensive analysis of Snakin-1 silenced lines demonstrated for the first time in potato that Snakin-1 plays a role in redox balance and participates in a complex crosstalk among different hormones.
- Published
- 2019
- Full Text
- View/download PDF
5. Biotechnological improvement of ornamental plants
- Author
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Flavia Soledad Darqui, Laura Mabel Radonic, Horacio Esteban Hopp, and Marisa Lopez Bilbao
- Subjects
GMO ,petunia ,rose ,chrysanthemum and carnation. ,Plant culture ,SB1-1110 - Abstract
The discovery of commercial transgenic varieties of orange petunias sold in Europe and the United States although they had never reached the approved status, and the consequent recommendation to destroy them, was the trigger to discuss about biotechnological improvement of ornamental plants. Inside the restricted world of 26 vegetal transgenic species, according to the ISAAA’s reports (http://www.isaaa.org), there are three ornamental species: carnation, rose and the Beijing University developed petunia; all of them with the same trait, a change in their colour. On the other hand, in 2014, the whole-genome sequence of carnation appeared which was the first and until now the only one among ornamental species. In this context, we review the publications from the last five years in petunia, rose, chrysanthemum and carnation. In these papers there are detailed descriptions of modification of the cascade of genes and transcription factors involved in stress situations, in different developmental stages and their regulation through different plant hormones. This knowledge will allow breeding for better and new varieties with changes in their abiotic or biotic stress tolerance, altered growth or yield and modified product quality as colour or fragrance.
- Published
- 2017
- Full Text
- View/download PDF
6. Optimizing ddRADseq in Non-Model Species: A Case Study in Eucalyptus dunnii Maiden
- Author
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Natalia Cristina Aguirre, Carla Valeria Filippi, Giusi Zaina, Juan Gabriel Rivas, Cintia Vanesa Acuña, Pamela Victoria Villalba, Martín Nahuel García, Sergio González, Máximo Rivarola, María Carolina Martínez, Andrea Fabiana Puebla, Michele Morgante, Horacio Esteban Hopp, Norma Beatriz Paniego, and Susana Noemí Marcucci Poltri
- Subjects
SNP ,SSR ,next generation sequencing ,genotyping by sequencing ,Agriculture - Abstract
Restriction site-associated DNA sequencing (RADseq) and its derived protocols, such as double digest RADseq (ddRADseq), offer a flexible and highly cost-effective strategy for efficient plant genome sampling. This has become one of the most popular genotyping approaches for breeding, conservation, and evolution studies in model and non-model plant species. However, universal protocols do not always adapt well to non-model species. Herein, this study reports the development of an optimized and detailed ddRADseq protocol in Eucalyptus dunnii, a non-model species, which combines different aspects of published methodologies. The initial protocol was established using only two samples by selecting the best combination of enzymes and through optimal size selection and simplifying lab procedures. Both single nucleotide polymorphisms (SNPs) and simple sequence repeats (SSRs) were determined with high accuracy after applying stringent bioinformatics settings and quality filters, with and without a reference genome. To scale it up to 24 samples, we added barcoded adapters. We also applied automatic size selection, and therefore obtained an optimal number of loci, the expected SNP locus density, and genome-wide distribution. Reliability and cross-sequencing platform compatibility were verified through dissimilarity coefficients of 0.05 between replicates. To our knowledge, this optimized ddRADseq protocol will allow users to go from the DNA sample to genotyping data in a highly accessible and reproducible way.
- Published
- 2019
- Full Text
- View/download PDF
7. Main and epistatic QTL analyses for Sclerotinia Head Rot resistance in sunflower.
- Author
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Jeremías Enrique Zubrzycki, Carla Andrea Maringolo, Carla Valeria Filippi, Facundo José Quiróz, Verónica Nishinakamasu, Andrea Fabiana Puebla, Julio A Di Rienzo, Alberto Escande, Verónica Viviana Lia, Ruth Amalia Heinz, Horacio Esteban Hopp, Gerardo D L Cervigni, and Norma Beatriz Paniego
- Subjects
Medicine ,Science - Abstract
Sclerotinia Head Rot (SHR), a disease caused by Sclerotinia sclerotiorum, is one of the most limiting factors in sunflower production. In this study, we identified genomic loci associated with resistance to SHR to support the development of assisted breeding strategies. We genotyped 114 Recombinant Inbred Lines (RILs) along with their parental lines (PAC2 -partially resistant-and RHA266 -susceptible-) by using a 384 single nucleotide polymorphism (SNP) Illumina Oligo Pool Assay to saturate a sunflower genetic map. Subsequently, we tested these lines for SHR resistance using assisted inoculations with S. sclerotiorum ascospores. We also conducted a randomized complete-block assays with three replicates to visually score disease incidence (DI), disease severity (DS), disease intensity (DInt) and incubation period (IP) through four field trials (2010-2014). We finally assessed main effect quantitative trait loci (M-QTLs) and epistatic QTLs (E-QTLs) by composite interval mapping (CIM) and mixed-model-based composite interval mapping (MCIM), respectively. As a result of this study, the improved map incorporates 61 new SNPs over candidate genes. We detected a broad range of narrow sense heritability (h2) values (1.86-59.9%) as well as 36 M-QTLs and 13 E-QTLs along 14 linkage groups (LGs). On LG1, LG10, and LG15, we repeatedly detected QTLs across field trials; which emphasizes their putative effectiveness against SHR. In all selected variables, most of the identified QTLs showed high determination coefficients, associated with moderate to high heritability values. Using markers shared with previous Sclerotinia resistance studies, we compared the QTL locations in LG1, LG2, LG8, LG10, LG11, LG15 and LG16. This study constitutes the largest report of QTLs for SHR resistance in sunflower. Further studies focusing on the regions in LG1, LG10, and LG15 harboring the detected QTLs are necessary to identify causal alleles and contribute to unraveling the complex genetic basis governing the resistance.
- Published
- 2017
- Full Text
- View/download PDF
8. Unveiling the genetic basis of Sclerotinia head rot resistance in sunflower
- Author
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Ruth Amelia Heinz, J. A. Di Rienzo, Andrea F. Puebla, Carla Maringolo, Facundo José Quiroz, Norma Beatriz Paniego, Verónica Viviana Lia, Carla Valeria Filippi, Horacio Esteban Hopp, Alberto Escande, Daniel Alvarez, and J. E. Zubrzycki
- Subjects
Germplasm ,Candidate gene ,ARGENTINIAN GERMPLASM ,Genotype ,Population ,Plant Science ,SUNFLOWER ,Biology ,Plant disease resistance ,Polymorphism, Single Nucleotide ,ASSOCIATION MAPPING ,Ascomycota ,lcsh:Botany ,Enfermedades de las Plantas ,Genetics ,DISEASE RESISTANCE ,Girasol ,Association mapping ,education ,Rots ,Alleles ,Genetic Association Studies ,Sclerotinia ,Plant Diseases ,education.field_of_study ,Disease resistance ,Sclerotinia sclerotiorum ,Chromosome Mapping ,purl.org/becyt/ford/4.4 [https] ,Helianthus annuus ,biology.organism_classification ,Sunflower ,Genética ,Podredumbres ,lcsh:QK1-989 ,Plant Breeding ,Phenotype ,Resistencia a la Enfermedad ,Argentinian germplasm ,Helianthus ,purl.org/becyt/ford/4 [https] ,Microsatellite Repeats ,Research Article - Abstract
Background: Sclerotinia sclerotiorum is a necrotrophic fungus that causes Sclerotinia head rot (SHR) in sunflower, with epidemics leading to severe yield losses. In this work, we present an association mapping (AM) approach to investigate the genetic basis of natural resistance to SHR in cultivated sunflower, the fourth most widely grown oilseed crop in the world. Results: Our association mapping population (AMP), which comprises 135 inbred breeding lines (ILs), was genotyped using 27 candidate genes, a panel of 9 Simple Sequence Repeat (SSR) markers previously associated with SHR resistance via bi-parental mapping, and a set of 384 SNPs located in genes with molecular functions related to stress responses. Moreover, given the complexity of the trait, we evaluated four disease descriptors (i.e, disease incidence, disease severity, area under the disease progress curve for disease incidence, and incubation period). As a result, this work constitutes the most exhaustive AM study of disease resistance in sunflower performed to date. Mixed linear models accounting for population structure and kinship relatedness were used for the statistical analysis of phenotype-genotype associations, allowing the identification of 13 markers associated with disease reduction. The number of favourable alleles was negatively correlated to disease incidence, disease severity and area under the disease progress curve for disease incidence, whereas it was positevily correlated to the incubation period. Conclusions: Four of the markers identified here as associated with SHR resistance (HA1848, HaCOI_1, G33 and G34) validate previous research, while other four novel markers (SNP117, SNP136, SNP44, SNP128) were consistently associated with SHR resistance, emerging as promising candidates for marker-assisted breeding. From the germplasm point of view, the five ILs carrying the largest combination of resistance alleles provide a valuable resource for sunflower breeding programs worldwide. Fil: Filippi, Carla Valeria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Zubrzycki, Jeremías Enrique. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Di Rienzo, Julio Alejandro. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina Fil: Quiroz, Facundo José. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina Fil: Puebla, Andrea Fabiana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Alvarez, D.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Manfredi; Argentina Fil: Maringolo, C. A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina Fil: Escande, Alberto Raul. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Lia, Veronica Viviana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
- Published
- 2020
- Full Text
- View/download PDF
9. Citrus Genetic Transformation: An Overview of the Current Strategies and Insights on the New Emerging Technologies
- Author
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Beatriz Xoconostle-Cázares, Horacio Esteban Hopp, Gabriela Conti, Gabriel Marcelino-Pérez, and Carina Andrea Reyes
- Subjects
Citrus ,Biotecnología ,Cisgenesis ,Emerging technologies ,Process (engineering) ,Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Interespaciadas ,Cisgénesis ,Biología ,Transformation methods ,Plant Science ,Review ,Biology ,Regeneración in Vitro ,Plantas Transgénicas ,Citrus transgenic plants ,SB1-1110 ,Genome editing ,CRISPR ,Ciencias Agrarias ,Transgenic Plants ,Selection (genetic algorithm) ,Citrus promoters ,CRISPR in citrus ,business.industry ,Intragénesis ,Plant culture ,food and beverages ,Cisgenesis and intragenesis ,Biotechnology ,Genetically modified organism ,New Technology ,Transformation (genetics) ,Genes Indicadores ,In vitro regeneration ,Intragenesis ,Reporter and selection markers ,business ,Citrus biotechnology ,Tecnología Nueva ,Reporter Genes - Abstract
Citrus are among the most prevailing fruit crops produced worldwide. The implementation of effective and reliable breeding programs is essential for coping with the increasing demands of satisfactory yield and quality of the fruit as well as to deal with the negative impact of fast-spreading diseases. Conventional methods are time-consuming and of difficult application because of inherent factors of citrus biology, such as their prolonged juvenile period and a complex reproductive stage, sometimes presenting infertility, self-incompatibility, parthenocarpy, or polyembryony. Moreover, certain desirable traits are absent from cultivated or wild citrus genotypes. All these features are challenging for the incorporation of the desirable traits. In this regard, genetic engineering technologies offer a series of alternative approaches that allow overcoming the difficulties of conventional breeding programs. This review gives a detailed overview of the currently used strategies for the development of genetically modified citrus. We describe different aspects regarding genotype varieties used, including elite cultivars or extensively used scions and rootstocks. Furthermore, we discuss technical aspects of citrus genetic transformation procedures via Agrobacterium, regular physical methods, and magnetofection. Finally, we describe the selection of explants considering young and mature tissues, protoplast isolation, etc. We also address current protocols and novel approaches for improving the in vitro regeneration process, which is an important bottleneck for citrus genetic transformation. This review also explores alternative emerging transformation strategies applied to citrus species such as transient and tissue localized transformation. New breeding technologies, including cisgenesis, intragenesis, and genome editing by clustered regularly interspaced short palindromic repeats (CRISPR), are also discussed. Other relevant aspects comprising new promoters and reporter genes, marker-free systems, and strategies for induction of early flowering, are also addressed. We provided a future perspective on the use of current and new technologies in citrus and its potential impact on regulatory processes., Instituto de Biotecnología y Biología Molecular
- Published
- 2021
10. Optimizing DDRADseq in non-model species: A Case Study in Eucalyptus dunnii Maiden
- Author
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Juan Gabriel Rivas, Norma Paniego, Pamela V. Villalba, Maximo Rivarola, Maria Carolina Martinez, Susana N. Marcucci Poltri, Carla Valeria Filippi, Cintia V. Acuña, Andrea F. Puebla, Michele Morgante, Natalia Cristina Aguirre, Horacio Esteban Hopp, Martín N. Garcia, Sergio González, and Giusi Zaina
- Subjects
Genetic Markers ,0106 biological sciences ,Computer science ,Genotypes ,Genotipos ,SNP ,Locus (genetics) ,Genotyping by sequencing ,Next generation sequencing ,SSR ,Computational biology ,01 natural sciences ,Genome ,Secuencia de ADN ,DNA sequencing ,Eucalyptus dunnii ,lcsh:Agriculture ,03 medical and health sciences ,DNA Sequence ,Genotyping ,030304 developmental biology ,Eucalyptus ,0303 health sciences ,lcsh:S ,Polimorfismo de un Solo Nucleótido ,Marcadores Genéticos ,Genetic marker ,Single Nucleotide Polymorphism ,Microsatellite ,Agronomy and Crop Science ,010606 plant biology & botany ,Reference genome - Abstract
Restriction site-associated DNA sequencing (RADseq) and its derived protocols, such as double digest RADseq (ddRADseq), offer a flexible and highly cost-effective strategy for efficient plant genome sampling. This has become one of the most popular genotyping approaches for breeding, conservation, and evolution studies in model and non-model plant species. However, universal protocols do not always adapt well to non-model species. Herein, this study reports the development of an optimized and detailed ddRADseq protocol in Eucalyptus dunnii, a non-model species, which combines different aspects of published methodologies. The initial protocol was established using only two samples by selecting the best combination of enzymes and through optimal size selection and simplifying lab procedures. Both single nucleotide polymorphisms (SNPs) and simple sequence repeats (SSRs) were determined with high accuracy after applying stringent bioinformatics settings and quality filters, with and without a reference genome. To scale it up to 24 samples, we added barcoded adapters. We also applied automatic size selection, and therefore obtained an optimal number of loci, the expected SNP locus density, and genome-wide distribution. Reliability and cross-sequencing platform compatibility were verified through dissimilarity coefficients of 0.05 between replicates. To our knowledge, this optimized ddRADseq protocol will allow users to go from the DNA sample to genotyping data in a highly accessible and reproducible way. Instituto de Biotecnología Fil: Aguirre, Natalia Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Filippi, Carla Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Zaina, Giusi. University of Udine. Department of Agricultural, Food, Environmental and Animal Sciences; Italia Fil: Rivas, Juan Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Acuña, Cintia Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Villalba, Pamela Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Garcia, Martin Nahuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gonzalez, Sergio Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rivarola, Maximo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Martinez, Maria Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Puebla, Andrea Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Morgante, Michele. University of Udine. Department of Agricultural, Food, Environmental and Animal Sciences; Italia Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Marcucci Poltri, Susana Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
- Published
- 2019
11. Biotechnological improvement of ornamental plants
- Author
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Laura M. Radonic, Flavia Soledad Darqui, Marisa López Bilbao, and Horacio Esteban Hopp
- Subjects
GMO, petunia, rose, chrysanthemum and carnation ,0301 basic medicine ,Fitomejoramiento ,Biotecnología ,Ornamental Plants ,Plant Science ,Carnation ,Orange (colour) ,Plantas Ornamentales ,lcsh:Plant culture ,Horticulture ,Petunia ,03 medical and health sciences ,Ornamental plant ,lcsh:SB1-1110 ,Plant breeding ,Abiotic component ,biology ,Genetically Modified Organisms ,Biotic stress ,biology.organism_classification ,Genetically modified organism ,Plant Breeding ,030104 developmental biology ,Organismos Modificados Genéticamente ,Biotechnology - Abstract
The discovery of commercial transgenic varieties of orange petunias sold in Europe and the United States although they had never reached the approved status, and the consequent recommendation to destroy them, was the trigger to discuss about biotechnological improvement of ornamental plants. Inside the restricted world of 26 vegetal transgenic species, according to the ISAAA’s reports (http://www.isaaa.org), there are three ornamental species: carnation, rose and the Beijing University developed petunia; all of them with the same trait, a change in their colour. On the other hand, in 2014, the whole-genome sequence of carnation appeared which was the first and until now the only one among ornamental species. In this context, we review the publications from the last five years in petunia, rose, chrysanthemum and carnation. In these papers there are detailed descriptions of modification of the cascade of genes and transcription factors involved in stress situations, in different developmental stages and their regulation through different plant hormones. This knowledge will allow breeding for better and new varieties with changes in their abiotic or biotic stress tolerance, altered growth or yield and modified product quality as colour or fragrance. A descoberta de variedades transgênicas de petúnias laranja vendidas na Europa e nos Estados Unidos que nunca alcançaram o status aprovado e a consequente recomendação de destruí-las foi o fator desencadeador para a discussão sobre a melhoria biotecnológica de plantas ornamentais. Dentro do mundo estrito de 26 espécies transgênicas vegetais, de acordo com os relatórios do ISAAA (http://www.isaaa.org), existem três espécies ornamentais: cravo, rosa e as petúnias desenvolvidas pela Universidade de Pequim que tem como característica mudança na cor. Por outro lado, em 2014 foi realizado pela primeira vez o sequenciamento completo do genoma do cravo que é o único sequenciado entre as espécies ornamentais. Neste contexto, revisamos as publicações dos últimos cinco anos em petúnia, rosa, crisântemo e cravo. Nestes trabalhos, há descrições detalhadas da modificação da cascata de genes e fatores de transcrição envolvidos em situações de estresse, diferentes estágios do crescimento e sua regulação através de diferentes hormônios vegetais. Este conhecimento contribuirá diretamente no melhoramento vegetal, o qual permitirá o desenvolvimento de novas variedades que sejam resistentes a diferentes situações de estresse abiótico ou biótico, alterações nos fatores que contribuem para o crescimento ou produtividade e modificações nos parâmetros de qualidade (como cor ou fragrância). Instituto de Biotecnología Fil: Darqui, Flavia Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Radonic, Laura Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: López Bilbao, Marisa Gisela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
- Published
- 2017
12. Population structure and genetic diversity characterization of a sunflower association mapping population using SSR and SNP markers
- Author
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Natalia Cristina Aguirre, Ruth A. Heinz, Verónica Viviana Lia, Daniel Alvarez, Jeremías Enrique Zubrzycki, Norma Paniego, Diego Cordes, Maria Valeria Moreno, Carla Valeria Filippi, Juan Gabriel Rivas, Corina M. Fusari, Andrea F. Puebla, and Horacio Esteban Hopp
- Subjects
Genetic Markers ,Germplasm ,Otras Biotecnología Agropecuaria ,Biotecnología Agropecuaria ,Population ,Argentina ,Marcadores Moleculares ,Population genetics ,SNP ,Plant Science ,Biology ,Polymorphism, Single Nucleotide ,Variación Genética ,Genetic variation ,Genetics ,Genetic variability ,Girasol ,education ,Association mapping ,Genetic resources ,Expressed Sequence Tags ,education.field_of_study ,Genetic diversity ,Polymorphism, Genetic ,Germoplasma ,Genetic Variation ,food and beverages ,purl.org/becyt/ford/4.4 [https] ,Bayes Theorem ,Genética ,SSR ,Helianthus Annuus ,Plant Breeding ,Marcadores Genéticos ,Genetics, Population ,CIENCIAS AGRÍCOLAS ,Multivariate Analysis ,Sunflower breeding ,Helianthus ,Microsatellite ,purl.org/becyt/ford/4 [https] ,Microsatellite Repeats ,Research Article - Abstract
Background: Argentina has a long tradition of sunflower breeding, and its germplasm is a valuable genetic resource worldwide. However, knowledge of the genetic constitution and variability levels of the Argentinean germplasm is still scarce, rendering the global map of cultivated sunflower diversity incomplete. In this study, 42 microsatellite loci and 384 single nucleotide polymorphisms (SNPs) were used to characterize the first association mapping population used for quantitative trait loci mapping in sunflower, along with a selection of allied open-pollinated and composite populations from the germplasm bank of the National Institute of Agricultural Technology of Argentina. The ability of different kinds of markers to assess genetic diversity and population structure was also evaluated. Results: The analysis of polymorphism in the set of sunflower accessions studied here showed that both the microsatellites and SNP markers were informative for germplasm characterization, although to different extents. In general, the estimates of genetic variability were moderate. The average genetic diversity, as quantified by the expected heterozygosity, was 0.52 for SSR loci and 0.29 for SNPs. Within SSR markers, those derived from non-coding regions were able to capture higher levels of diversity than EST-SSR. A significant correlation was found between SSR and SNP- based genetic distances among accessions. Bayesian and multivariate methods were used to infer population structure. Evidence for the existence of three different genetic groups was found consistently across data sets (i.e., SSR, SNP and SSR + SNP), with the maintainer/restorer status being the most prevalent characteristic associated with group delimitation. Conclusion: The present study constitutes the first report comparing the performance of SSR and SNP markers for population genetics analysis in cultivated sunflower. We show that the SSR and SNP panels examined here, either used separately or in conjunction, allowed consistent estimations of genetic diversity and population structure in sunflower breeding materials. The generated knowledge about the levels of diversity and population structure of sunflower germplasm is an important contribution to this crop breeding and conservation. Instituto de Biotecnología Fil: Filippi, Carla Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Aguirre, Natalia Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Rivas, Juan Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Zubrzycki, Jeremias Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Puebla, Andrea Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Cordes, Diego Darío. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina Fil: Moreno, Maria Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina Fil: Fusari, Corina Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Max Planck Institute of Molecular Plant Physiology; Alemania Fil: Alvarez, Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina Fil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lia, Veronica Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
- Published
- 2014
- Full Text
- View/download PDF
13. Genetic characterization of sunflower breeding resources from Argentina: assessing diversity in key open-pollinated and composite populations
- Author
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M. A. Loray, Verónica Viviana Lia, Horacio Esteban Hopp, Verónica Nishinakamasu, A. Vicario, Jorge O. Gieco, Daniel Alvarez, Maria Valeria Moreno, Norma Paniego, and Ruth A. Heinz
- Subjects
Germplasm ,Genetic diversity ,Otras Biotecnología Agropecuaria ,business.industry ,Biotecnología Agropecuaria ,Locus (genetics) ,MOLECULAR MARKERS ,Plant Science ,Biology ,Sunflower ,SSR ,CULTIVATED SUNFLOWER GERMPLASM ,Biotechnology ,Open pollination ,CIENCIAS AGRÍCOLAS ,Genetic variation ,Genetics ,Microsatellite ,GENETIC DIVERSITY ,Allele ,business ,Agronomy and Crop Science - Abstract
Open-pollinated (OPs) and composite populations (CPs) represent a valuable resource for sunflower breeding programmes. However, little is known about the levels and distribution of genetic variation within each of these populations. In this study, quantitative and qualitative traits along with molecular markers were used to evaluate 14 populations from the Instituto Nacional de Tecnología Agropecuaria (INTA) sunflower germplasm collection. These populations were chosen to represent historically important accessions that still play a central role within the INTA sunflower breeding programme due to their extensive variation in diverse agronomically important traits. Nine quantitative and eight qualitative agro-morphological descriptors were recorded and compared with those of a larger set of accessions representative of the phenotypic diversity of the sunflower collection. Molecular characterization was conducted on a total of 311 individuals using 16 microsatellite markers. Overall, the average gene diversity was 0.56 and the average number of alleles per locus was 6.25. No statistically significant differences in genetic diversity were detected between the OPs and CPs. Global estimates of F ST revealed very high levels of differentiation among accessions (F ST= 0.413, P< 0.05). Population structure analyses were consistent with the observed levels of differentiation and identified two major groups. The results of this work show that high global diversity is preserved within the accessions analysed here. Additionally, this study provides a set of reliable and discriminant markers for the cost-effective molecular characterization of sunflower accessions, along with the guidelines for the delineation of sampling strategies for OPs and CPs, thus aiding the efficient management and exploitation of sunflower germplasm collections. Fil: Moreno, M. V.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Manfredi; Argentina Fil: Nishinakamasu, V.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina Fil: Loray, M. A.. Instituto Nacional de Semillas; Argentina Fil: Alvarez, D.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Manfredi; Argentina Fil: Gieco, J.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Manfredi; Argentina Fil: Vicario, A.. Instituto Nacional de Semillas; Argentina Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina Fil: Heinz, Ruth Amelia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina Fil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lia, Verónica Viviana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
- Published
- 2013
14. Snakin/GASA proteins : involvement in hormone crosstalk and redox homeostasis
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Natalia Ines Almasia, Horacio Esteban Hopp, Cecilia Vazquez-Rovere, and Vanesa Nahirñak
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Mini Review ,Mutant ,Plant Development ,Gibberellic Acid ,Plant Science ,División Celular ,Redox Potential ,Biology ,Snakin-1 ,Protein structure ,Plant Growth Regulators ,Gene Expression Regulation, Plant ,Homeostasis ,Mode of action ,Plant Proteins ,Potencial Redox ,Regulation of gene expression ,Abiotic stress ,food and beverages ,Péptidos ,Abiotic Stress ,Subcellular localization ,Phenotype ,Cell biology ,Crosstalk (biology) ,Ácido Giberelico ,Estrés Abiótico ,Peptides ,Oxidation-Reduction ,Cell Division - Abstract
Snakin/GASA proteins are widely distributed among plant species. They are expressed in different plant organs with high tissue and temporal specificity, and their subcellular localization varies among the different members. Interestingly, all of them maintain 12 cysteines of the C-terminus in highly conserved positions of the aminoacid sequences that are essential for their biochemical activity and probably responsible for their protein structure. Despite their common features, their functions are not completely elucidated and little is known about their mode of action. This review focuses on the current knowledge about this intriguing family of peptides and advances comprising gene regulation analyses, expression pattern studies and phenotypic characterization of mutants and transgenic plants. Furthermore, we discuss the roles of Snakin/GASA proteins in several aspects of plant development, plant responses to biotic or abiotic stress and their participation in hormone crosstalk and redox homeostasis. Instituto de Biotecnología Fil: Nahirñak, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Almasia, Natalia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Vazquez Rovere, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
- Published
- 2012
- Full Text
- View/download PDF
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